59 results about "Arthrobacter" patented technology

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The invention relates to the technical field of environmental microorganisms, in particular to arthrobacterium Arth.R8 and application thereof, and the preservation number of the arthrobacterium Arth.R8 is GDMCC No.63567. The arthrobacter Arth.R8 has the effects of fixing nitrogen, dissolving organic phosphorus, promoting growth and promoting nodulation. The arthrobacter Arth.R8 disclosed by the invention can be used for promoting absorption and utilization of nutrient substances such as nitrogen and phosphorus in a plant growth process, inhibiting growth of fusarium graminearum and promoting plant growth, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention discloses a decay-promoting and carbon-retaining agent and a preparation method, application and a verification method thereof. The decay-promoting and carbon-retaining agent comprises a complex microbial inoculant and a nutrient preparation, the complex microbial inoculant comprises the following substances in parts by weight: 2 parts of aspergillus oryzae, 4 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 2 parts of lactic acid bacteria, 4 parts of Koxime saccharomycetes, 3 parts of acetobacter aceti, 4 parts of aspergillus candidus, 1 part of deuteromycetes and 1 part of arthrobacter; the nutrient preparation comprises the following substances in parts by weight: 5 parts of urea, 2 parts of diammonium phosphate and 1.3 parts of potassium sulfate. According to the method, the method for verifying the effect of mixed application of the straw and the decay-promoting and carbon-retaining agent to the soil is constructed, and the 13C isotope tracing technology is combined to verify the SOC immobilization effect of the decay-promoting and carbon-retaining agent on the soil with different textures, so that the situation that the decay-promoting and carbon-retaining agent with different components can achieve more effective SOC immobilization on the soil with the different textures is obtained, and straw resources are fully utilized.

The invention discloses arthrobacter gandavensis N-15 with high selenium resistance and application of the arthrobacter gandavensis N-15, the arthrobacter gandavensis N-15 with high selenium resistance is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242146, and the preservation date is October 9, 2024. The arthrobacter gandavensis N-15 has the advantages that the arthrobacter gandavensis N-15 can be used for preparing the selenium-resistant arthrobacter gandavensis N-15; the selenium resistance of the arthrobacter dabarangensis N-15 reaches 128 mmol / L, and the arthrobacter dabarangensis N-15 has stable adaptability to high selenium concentration and is convenient for biological preparation of nano-selenium. The arthrobacter dawlini N-15 can be used for reducing sodium selenite to generate nano-selenium, and the generated nano-selenium has certain ABTS free radical scavenging capacity and DPPH free radical scavenging capacity, that is, the nano-selenium has relatively good antioxidant capacity. The arthrobacter dawlini N-15 and the nano-selenium produced by the arthrobacter dawlini N-15 can be widely applied to the fields of medicine, food and agriculture.

The invention belongs to the technical field of biology, and relates to zymoprotein, in particular to recombinant arthrobacter globiforme uricase and application thereof. The recombinant arthrobacter globiforme uricase has single-site amino acid mutation, amino acids are located in an active region and an inactive region of the uricase, and the amino acid sequence of the recombinant arthrobacter globiforme uricase is shown as any one of SEQ ID NO: 1-8. Compared with wild uricase, the mutant uricase formed after amino acid is introduced into uricase at a fixed point has higher activity, and the use amount of uricase can be more effectively reduced under the condition that the content of uric acid is effectively controlled.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

The invention discloses arthrobacter AD8 capable of efficiently degrading atrazine and application of the arthrobacter AD8, the strain can efficiently degrade atrazine and is effectively used for repairing pollution caused by atrazine to soil and water, the classification name is Paenarthrobacter sp., and the arthrobacter AD8 is preserved in China General Microbiological Culture Collection Center on March 18, 2025, and has the preservation number of CGMCC NO. The address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.33865. The triazine herbicide has the advantages of high efficiency in degradation of atrazine in soil and water and remediation of atrazine-polluted water and soil, good effect and high degradation efficiency, is a great innovation in remediation of triazine herbicide-polluted water and soil, and has significant economic and social benefits.

The invention provides arthrobacter HJ1 for preventing and treating fungal root rot of polygonatum sibiricum and application of the arthrobacter HJ1, and belongs to the technical field of disease prevention and treatment. The preservation number of the arthrobacterium HJ1 is CCTCC (China Center For Type Culture Collection) NO: M20251843 The arthrobacter HJ1 has a remarkable inhibition effect on the pathogenic bacterium fusarium oxysporum of the rhizoma polygonati root rot. The arthrobacter HJ1 has a remarkable inhibition effect on the rhizoma polygonati root rot under an in-vitro condition, and can remarkably reduce scab expansion of the rhizoma polygonati root rot caused by fusarium oxysporum. In a field test, by using the arthrobacter HJ1, the morbidity of the rhizoma polygonati root rot can be remarkably reduced, and the control effect on the rhizoma polygonati root rot is improved. A safety test shows that the arthrobacter HJ1 has no adverse effect on the growth of the polygonatum sibiricum and can be safely used for preventing and controlling root rot fungal diseases of the polygonatum sibiricum. The arthrobacter HJ1, as a beneficial biocontrol bacterium for polygonatum sibiricum root rot fungal diseases, is of great significance to healthy and green development of the polygonatum sibiricum industry.

The invention discloses an application of a complex microbial inoculant in improving the effectiveness of nitrogen in peanut rhizosphere soil, and belongs to the technical field of microorganisms. According to the invention, the composite microbial agent composed of active bacteria, namely arthrobacter plantarum ARZ1 and sphingomonas melonis SPZ1, is applied to the field of peanut planting for the first time, and the two bacteria in the composite microbial agent have an obvious promoting effect on increasing the content of total nitrogen, nitrate nitrogen and ammoniacal nitrogen in rhizosphere soil of peanuts through a synergistic effect, so that the content of the total nitrogen, nitrate nitrogen and ammoniacal nitrogen in the rhizosphere soil of the peanuts is increased, and the content of the total nitrogen, nitrate nitrogen and ammoniacal nitrogen in the rhizosphere soil of the peanuts is increased; particularly, the fruit number, the fruit weight and the kernel weight of a single peanut plant can be increased, and the peanut yield can be greatly increased in the peanut planting process.

The invention relates to a preparation method of prednisone acetate. The preparation method comprises the following steps: carrying out secondary culture on arthrobacter simplex; adding a crude product of cortisone acetate into the arthrobacter simplex subjected to secondary culture, performing fermentation conversion, and performing conversion by adopting a mode of gradient temperature control and batch addition of an organic solvent to obtain a crude product of prednisone acetate; refining the prednisone acetate crude product, pulping with ethyl acetate, heating and refluxing, and repeatedly refining for multiple times to obtain a prednisone acetate refined product; and heating and dissolving the prednisone acetate refined product with ethyl acetate, adding activated carbon and water, heating and refluxing, filtering and concentrating to obtain the prednisone acetate refined product. According to the method disclosed by the invention, a mode of gradient temperature control and batch addition of the organic solvent is adopted during conversion, so that the strain conversion efficiency is improved, and the conversion rate of prednisone acetate reaches 94% or above; during refining, a pulping mode is adopted, so that the batch feeding amount is increased, a repeated refining and concentrating process is removed, the solvent consumption is reduced, and the process time is greatly shortened.

The invention provides an arthrobotrys oligospora genetic engineering strain with nematode attracting activity, and belongs to the technical field of microbial genetic engineering, the genetic engineering strain takes arthrobotrys oligospora as a starting strain, and the genetic engineering strain is a strain obtained by knocking out an AoETF beta gene in an arthrobotrys oligospora genome. The arthrobotrys oligospora genetic engineering strain has nematode attraction activity, and can attract nematodes in soil to gather together so as to achieve the effect of efficiently capturing and killing the nematodes, thereby providing a basis for biological control of the nematodes. The invention further provides a construction method and application of the arthrobotrys oligospora genetic engineering strain with nematode attracting activity.

The application belongs to the field of microorganisms and the field of environmental protection, and provides at least one urea-producing Paenarthrobacter ureafaciens for degrading atrazine, wherein the urea-producing Paenarthrobacter ureafaciens is preserved in the China General Microbiological Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, located at No. 1, Xiliujia, Beijing Chaoyang District, and has the preservation numbers of CGMCC No. 37944 or CGMCC No. 37943, respectively. The application obtains the urea-producing Paenarthrobacter ureafaciens with high efficiency, wide spectrum and strong environmental adaptability through screening, and finally realizes rapid and complete repair of atrazine contaminated soil by matching an optimized microbial agent formula and a preparation process, so as to reduce repair cost, promote the large-scale application of the microbial repair technology in farmland and industrial pollution sites, and solve many technical bottlenecks in the field of atrazine pollution repair.

The invention belongs to the technical field of gene engineering, and particularly relates to an arthrobacter carbodii gene knockout vector and method. The vector comprises a target gene upstream and downstream homologous arm amplification sequence and a target gene double PAM integration fragment; wherein the target gene is a gene segment to be knocked out; the lengths of upstream and downstream arms of the target gene are respectively 1000bp, and the lengths of amplified homologous arm genes are respectively 2000bp; a basic group at the tail of the sequence of the double PAM integrated fragment is adenine or guanine, and the GC content is 40-60%. According to the knockout vector and the corresponding knockout method, the stable knockout of the gene in the Arthrobacter carbodii can be realized, the knockout efficiency is high, and the knockout vector and the corresponding knockout method have important significance for researching the gene function of the Arthrobacter carbodii and also have a certain reference value for other strains of the Arthrobacter.

The invention belongs to the technical field of soil remediation, and particularly relates to an alpine mining area root-promoting bacterium solution and a preparation method and application thereof. The strains in the root-promoting bacterium liquid disclosed by the invention comprise sphingomonas, arthrobacter, bacillus, motility coccus and red bacillus. Results of the embodiment of the invention show that the root system and plant height of elymus dahuricus hydroponically cultured by using the root-promoting bacterium solution are obviously increased. Furthermore, the combination of the root-promoting bacterial liquid and elymus dahuricus can significantly improve the soil components of the high and cold mining area, thereby promoting the restoration of the barren soil of the high and cold mining area.

This invention relates to Nicotine-like Arthrobacter or contain Nicotine Arthroblasts The use of bacterial combinations for the degradation of one or more alkaloids in processed tobacco.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.

The invention discloses a degrading bacterium system for repairing pesticide-polluted soil and application of the degrading bacterium system. The degrading bacterial system is composed of Pseudomonas plecoglossa JY-5, Klebsiella plecoglossa J2-1, Arthrobacter silvisoli JY-2 and Enterobacter ludwigii JY-1, and the degrading bacterial system is obtained by carrying out enrichment culture, screening, sequencing analysis and bacterial strain identification and purification on the soil polluted by the pesticide, and the degrading bacterial system is used for degrading the soil polluted by the pesticide, and the degrading bacterial system is used for degrading the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide. According to the degrading bacterium system, the biochar is used as a carrier to prepare an immobilized bacterium agent, various common pesticides such as carbendazim and the like can be efficiently degraded, the strains have a synergistic effect, the stability in a complex soil environment is high, the anti-interference capability is outstanding, soil pesticide residues can be effectively reduced, an efficient and feasible technical scheme is provided for soil remediation in a pesticide high-pollution area, and the degrading bacterium system has wide application prospects. The method has important practical application value.

This invention provides an acid- and alkali-producing bacterial combination, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a bacterial combination including Bacillus subtilis with accession number CGMCC No. 29201. Bacillus sp S1 and Arthrobacterium with accession number CGMCC No. 29202 ( Arthrobacter sp S2. In this invention, both Bacillus S1 and Arthrobacter S2 are acid- and alkali-producing strains with strong environmental adaptability. Through synergistic effects via multiple pathways, including ammonia production, bicarbonate secretion, and activation of soil enzymes, the bacterial combination can significantly increase soil pH, reduce exchangeable aluminum and hydrolyzable acids, and simultaneously increase soil bicarbonate content, thus rapidly and comprehensively improving soil acidity from its root cause. This bacterial combination is of great significance for achieving green, efficient, and sustainable remediation of acidified soils.

The invention provides arthrobacter ZJUHUYJH1 and application thereof in preventing and treating gramineous meloidogyne, and belongs to the technical field of prevention and treatment of plant meloidogyne. The arthrobacter ZJUHUYJH1 provided by the invention has the preservation number of CCTCC NO: M 2025922, is separated from rice root soil infected by the gramineous meloidogyne, and has the advantages that the preservation number is CCTCC NO: M 2025922; the composition has a good prevention and treatment effect on the gramineous meloidogyne; the correction fatality rate of second-stage larvae of the meloidogyne reaches 91.4%, and the root knot number and the ratio of the root knot number to the dry weight of rice infected by the pseudogramineous meloidogyne can be reduced; the method has potential and practical application value in research of the Meloidogyne pseudoaceae.

The invention belongs to the technical field of microorganism application, and particularly relates to an alpine mining area plant stress-resistant microbial agent as well as a preparation method and application thereof. The plant stress-resistant microbial agent is prepared by mixing freeze-dried bacterial powder prepared from sphingomonas, arthrobacter, bacillus, red bacillus and marinobacter, pretreated kitchen garbage and an adhesive according to a proportion. The freeze-dried bacterial powder is prepared from sphingomonas, arthrobacter, bacillus, red bacillus and marinobacter. Results of the embodiment of the invention show that the plant stress-resistant microbial agent can significantly promote plant growth, and the viable bacteria retention rate is 50% or above. Furthermore, the plant stress-resistant microbial agent and elymus dahuricus are combined for use, so that the remediation capability of the barren soil in the high and cold mining area can be improved.

The invention relates to the technical field of microorganisms and biological fertilizers, in particular to a vicia sativa growth-promoting and stress-resisting complex microbial inoculant and a preparation method thereof.The complex microbial inoculant is formed by mixing arthrobacter, bacillus pumilus, bacillus flexus and brevundimonas intermedia according to the proportion of the arthrobacter, the bacillus pumilus, the bacillus flexus and the brevundimonas intermedia, and the effective viable count in the complex microbial inoculant is 1 * 10 < 9 >-10 < 10 > CFU / g; the synthetic bacterial agent produced by the invention can be applied to the planting and production process of vicia sativa as bacterial fertilizer, and is convenient to apply and remarkable in growth promoting effect. The microbial agent not only can accelerate the accumulation of vicia sativa biomass and improve the production performance, but also can help vicia sativa to resist various environmental stresses, so that the viability of vicia sativa in a severe environment is guaranteed. The method has important significance for improving the production capacity of the common vetch and promoting the development of animal husbandry.

The invention belongs to the technical field of gene engineering, and particularly relates to a 3-ketosteroid-delta1-dehydrogenase mutant with improved heat resistance and an application of the 3-ketosteroid-delta1-dehydrogenase mutant. The 3-ketosterone-delta1-dehydrogenase AuKsdD gene derived from arthrobacter urate oxydans is subjected to structural analysis and site-directed mutagenesis design, and a mutant with higher catalytic activity and remarkably enhanced heat resistance compared with a wild type AuKsdD is obtained through directed mutation of key sites E244, K267, T301 and A335. The mutant can keep high catalytic efficiency for a long time under a high-temperature condition, and is suitable for industrial synthesis of steroid drug raw materials.

The application provides a strain of Arthrobacter sp. ZJUHUYJH1 and application thereof in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of prevention and treatment of plant root-knot nematodes. The Arthrobacter sp. ZJUHUYJH1 provided by the application has a preservation number of CCTCC NO: M 2025922, is isolated from rice rhizosphere soil infected by paratyphaceae root-knot nematodes, has a good prevention and treatment effect on paratyphaceae root-knot nematodes, a rectification mortality of 91.4% on second instar larvae of root-knot nematodes, can reduce the number of root-knots and the ratio of the number of root-knots to dry weight of rice infected by paratyphaceae root-knot nematodes, and has potential and practical application value in the research of paratyphaceae root-knot nematodes.

The application discloses a kind of based on whole solid waste carbon reduction utilization's goaf stable filling system and filling method, the system includes biological preparation culture system, solid waste prefabrication system, gas injection system and goaf, the biological preparation culture system includes two incubators, and solid waste prefabrication system includes two prefabricators.Acid bacillus, arthrobacter are respectively added into two incubators and are respectively entered into two prefabricators after cultivation, steel slag is added into the prefabricator of acid bacillus bacterial solution, calcium carbide slag, granulated blast furnace slag are added into the prefabricator of arthrobacter bacterial solution, obtain two kinds of solid waste-biological bacterial solution mixture injection mineral resources goaf, pass in carbon dioxide-air mixed gas reaction, complete the stable filling of goaf.The present application is glued into the filling body with certain strength by microorganism carbon fixation mineralization reaction and solid waste pozzolanic reaction, not only effectively utilizes greenhouse gas carbon dioxide and industrial waste, but also realizes the safe treatment of mineral resources goaf.

The invention discloses ammonia assimilation arthrobacter arylalani and application of the ammonia assimilation arthrobacter arylalani in aquaculture water treatment, and relates to the technical field of wastewater treatment, the ammonia assimilation arthrobacter arylalani is arthrobacter arylalani Aa01 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36829, and the preservation date is December 01, 2025. The invention also discloses application of the ammonia assimilation arthrobacter ashlii in aquaculture water treatment. The ammonia assimilation arthrobacter arabilis Aa01 provided by the invention is a microbial strain with remarkable characteristics and application value, the strain has the most remarkable characteristic of a unique nitrogen conversion mechanism, NH4 < + >-N, NO2 <->-N and NO3 <->-N can be efficiently used as electron acceptors for respiration under an aerobic condition, and the dependence of a traditional denitrification process on an anaerobic environment is broken through; compared with a conventional biological denitrification strain, the Aa01 strain shows excellent performance advantages.

The application discloses a microbial agent for improving cotton yield and quality and a preparation method thereof, and belongs to the technical field of organic fertilizer and microbial fertilizer manufacturing. The microbial agent for improving cotton yield and quality comprises the following raw materials in parts by weight: 50-60 parts of a compound microbial liquid, 8-10 parts of alginate oligosaccharide, 6-8 parts of humic acid chelated boron zinc, 4-5 parts of polyglutamic acid, 12-15 parts of modified attapulgite, and 2-3 parts of glycerol; the compound microbial liquid comprises Candida valensis, Arthrobacter mysorens, and Pseudomonas psychrophilus. The three strains are colonized in the rhizosphere of cotton together, synergistically promote the growth of cotton, make cotton seeds full, increase the yield of cotton, effectively improve the quality of lint, realize the dual targets of yield increase and quality improvement, reduce the use of chemical fertilizers and pesticides, and are a very promising technical scheme for promoting green, high-yield, high-quality and efficient production of cotton.

This invention provides a group of microbial flora for the degradation of short-chain phthalates and the repair of plant damage, belonging to the field of microbial technology. The aforementioned microbial flora includes *Arthrobacter* (…). Paenarthrobacter sp.) PAEs3-4, Corynebacterium glutamicum (sp.) Glutamicibacter sp.) A4 and Pseudomonas aeruginosa (sp.) A4 and Pseudomonas aeruginosa ( Pseudomonas Paenarthrobacter sp. 60231 and Paenarthrobacter sp. PAEs3-4 were deposited at the China Center for Type Culture Collection (CCTCC) on January 16, 2026, with accession number CCTCC M 2026142. These bacterial groups can achieve plant damage repair during growth while ensuring the degradation of short-chain PAEs.

This invention belongs to the field of microbial technology and relates to a salt-tolerant Arthrobacter strain, its microecological preparations, and applications. The invention uses a salt-tolerant Arthrobacter strain derived from the rhizosphere of aquatic plants as the starting strain. Through ultraviolet mutagenesis, a new strain producing high levels of indoleacetic acid was obtained. This strain has been deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number: CGMCC No. 30670. This bacterium possesses the ability to produce high levels of indoleacetic acid and can promote the growth of aquatic plants.

The present invention discloses a method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation, belonging to the field of environmental microbiological technology. In the present invention, pre-cultured Arthrobacter and Bacillus megaterium are prepared into a composite bacterial agent and inoculated into erucamide wastewater, and a biological enhancement treatment is implemented by adopting a staged aeration regulation strategy: in stage I, high aeration conditions are adopted to activate the rapid proliferation of bacteria and induce the expression of amidase, and in stage II, the aeration conditions are adjusted to low aeration conditions to optimize the anoxic environment for deamidation reaction. The present invention realizes the spatio-temporal synergistic effect of two functional bacteria by regulating the dissolved oxygen concentration, that is, Bacillus megaterium preferentially degrades ammonia nitrogen pollutants, and Arthrobacter specifically catalyzes the cleavage of the amide bond of erucamide, and their metabolic pathways form a complementarity; this process conforms to the principle of green chemistry and no secondary pollutants are generated, providing a new efficient and sustainable biological treatment approach for refractory organic wastewater containing erucamide.

The invention relates to a complex microbial inoculant for preventing and treating tobacco bacterial wilt and application of the complex microbial inoculant. Strain in the complex microbial inoculant comprises a mulberry enterobacter 65D6 strain, a Ralstonia 56D2 strain, an agrobacterium 18A6 strain, an arthrobacter uretogenes 05-507 strain and a bacillus safensis 05-2101 strain. The five specific strains are compounded and combined for use, an excellent tobacco bacterial wilt prevention and treatment effect is achieved, and experimental research shows that the disease occurrence of tobacco plants treated by the complex microbial inoculant is obviously slowed down, and the disease index is obviously lower than that of a clear water control group and that of a thiediazole copper and chloriure.copper sulfate chemical agent control group. Meanwhile, the five strains can be mutually matched and promoted, a synergistic effect is achieved in the aspect of preventing and treating tobacco bacterial wilt, the WY11 strain is further added for combined use, the effect of preventing and treating the tobacco bacterial wilt is improved, and the better effect of promoting the growth of tobacco plants can be achieved.