41 results about "Arthrobacter" patented technology

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The invention provides arthrobacter HJ1 for preventing and treating fungal root rot of polygonatum sibiricum and application of the arthrobacter HJ1, and belongs to the technical field of disease prevention and treatment. The preservation number of the arthrobacterium HJ1 is CCTCC (China Center For Type Culture Collection) NO: M20251843 The arthrobacter HJ1 has a remarkable inhibition effect on the pathogenic bacterium fusarium oxysporum of the rhizoma polygonati root rot. The arthrobacter HJ1 has a remarkable inhibition effect on the rhizoma polygonati root rot under an in-vitro condition, and can remarkably reduce scab expansion of the rhizoma polygonati root rot caused by fusarium oxysporum. In a field test, by using the arthrobacter HJ1, the morbidity of the rhizoma polygonati root rot can be remarkably reduced, and the control effect on the rhizoma polygonati root rot is improved. A safety test shows that the arthrobacter HJ1 has no adverse effect on the growth of the polygonatum sibiricum and can be safely used for preventing and controlling root rot fungal diseases of the polygonatum sibiricum. The arthrobacter HJ1, as a beneficial biocontrol bacterium for polygonatum sibiricum root rot fungal diseases, is of great significance to healthy and green development of the polygonatum sibiricum industry.

The invention relates to a preparation method of prednisone acetate. The preparation method comprises the following steps: carrying out secondary culture on arthrobacter simplex; adding a crude product of cortisone acetate into the arthrobacter simplex subjected to secondary culture, performing fermentation conversion, and performing conversion by adopting a mode of gradient temperature control and batch addition of an organic solvent to obtain a crude product of prednisone acetate; refining the prednisone acetate crude product, pulping with ethyl acetate, heating and refluxing, and repeatedly refining for multiple times to obtain a prednisone acetate refined product; and heating and dissolving the prednisone acetate refined product with ethyl acetate, adding activated carbon and water, heating and refluxing, filtering and concentrating to obtain the prednisone acetate refined product. According to the method disclosed by the invention, a mode of gradient temperature control and batch addition of the organic solvent is adopted during conversion, so that the strain conversion efficiency is improved, and the conversion rate of prednisone acetate reaches 94% or above; during refining, a pulping mode is adopted, so that the batch feeding amount is increased, a repeated refining and concentrating process is removed, the solvent consumption is reduced, and the process time is greatly shortened.

The invention provides an arthrobotrys oligospora genetic engineering strain with nematode attracting activity, and belongs to the technical field of microbial genetic engineering, the genetic engineering strain takes arthrobotrys oligospora as a starting strain, and the genetic engineering strain is a strain obtained by knocking out an AoETF beta gene in an arthrobotrys oligospora genome. The arthrobotrys oligospora genetic engineering strain has nematode attraction activity, and can attract nematodes in soil to gather together so as to achieve the effect of efficiently capturing and killing the nematodes, thereby providing a basis for biological control of the nematodes. The invention further provides a construction method and application of the arthrobotrys oligospora genetic engineering strain with nematode attracting activity.

The application belongs to the field of microorganisms and the field of environmental protection, and provides at least one urea-producing Paenarthrobacter ureafaciens for degrading atrazine, wherein the urea-producing Paenarthrobacter ureafaciens is preserved in the China General Microbiological Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, located at No. 1, Xiliujia, Beijing Chaoyang District, and has the preservation numbers of CGMCC No. 37944 or CGMCC No. 37943, respectively. The application obtains the urea-producing Paenarthrobacter ureafaciens with high efficiency, wide spectrum and strong environmental adaptability through screening, and finally realizes rapid and complete repair of atrazine contaminated soil by matching an optimized microbial agent formula and a preparation process, so as to reduce repair cost, promote the large-scale application of the microbial repair technology in farmland and industrial pollution sites, and solve many technical bottlenecks in the field of atrazine pollution repair.

The invention belongs to the technical field of gene engineering, and particularly relates to an arthrobacter carbodii gene knockout vector and method. The vector comprises a target gene upstream and downstream homologous arm amplification sequence and a target gene double PAM integration fragment; wherein the target gene is a gene segment to be knocked out; the lengths of upstream and downstream arms of the target gene are respectively 1000bp, and the lengths of amplified homologous arm genes are respectively 2000bp; a basic group at the tail of the sequence of the double PAM integrated fragment is adenine or guanine, and the GC content is 40-60%. According to the knockout vector and the corresponding knockout method, the stable knockout of the gene in the Arthrobacter carbodii can be realized, the knockout efficiency is high, and the knockout vector and the corresponding knockout method have important significance for researching the gene function of the Arthrobacter carbodii and also have a certain reference value for other strains of the Arthrobacter.

This invention relates to Nicotine-like Arthrobacter or contain Nicotine Arthroblasts The use of bacterial combinations for the degradation of one or more alkaloids in processed tobacco.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.

The invention discloses a degrading bacterium system for repairing pesticide-polluted soil and application of the degrading bacterium system. The degrading bacterial system is composed of Pseudomonas plecoglossa JY-5, Klebsiella plecoglossa J2-1, Arthrobacter silvisoli JY-2 and Enterobacter ludwigii JY-1, and the degrading bacterial system is obtained by carrying out enrichment culture, screening, sequencing analysis and bacterial strain identification and purification on the soil polluted by the pesticide, and the degrading bacterial system is used for degrading the soil polluted by the pesticide, and the degrading bacterial system is used for degrading the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide. According to the degrading bacterium system, the biochar is used as a carrier to prepare an immobilized bacterium agent, various common pesticides such as carbendazim and the like can be efficiently degraded, the strains have a synergistic effect, the stability in a complex soil environment is high, the anti-interference capability is outstanding, soil pesticide residues can be effectively reduced, an efficient and feasible technical scheme is provided for soil remediation in a pesticide high-pollution area, and the degrading bacterium system has wide application prospects. The method has important practical application value.

This invention provides an acid- and alkali-producing bacterial combination, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a bacterial combination including Bacillus subtilis with accession number CGMCC No. 29201. Bacillus sp S1 and Arthrobacterium with accession number CGMCC No. 29202 ( Arthrobacter sp S2. In this invention, both Bacillus S1 and Arthrobacter S2 are acid- and alkali-producing strains with strong environmental adaptability. Through synergistic effects via multiple pathways, including ammonia production, bicarbonate secretion, and activation of soil enzymes, the bacterial combination can significantly increase soil pH, reduce exchangeable aluminum and hydrolyzable acids, and simultaneously increase soil bicarbonate content, thus rapidly and comprehensively improving soil acidity from its root cause. This bacterial combination is of great significance for achieving green, efficient, and sustainable remediation of acidified soils.

The invention provides arthrobacter ZJUHUYJH1 and application thereof in preventing and treating gramineous meloidogyne, and belongs to the technical field of prevention and treatment of plant meloidogyne. The arthrobacter ZJUHUYJH1 provided by the invention has the preservation number of CCTCC NO: M 2025922, is separated from rice root soil infected by the gramineous meloidogyne, and has the advantages that the preservation number is CCTCC NO: M 2025922; the composition has a good prevention and treatment effect on the gramineous meloidogyne; the correction fatality rate of second-stage larvae of the meloidogyne reaches 91.4%, and the root knot number and the ratio of the root knot number to the dry weight of rice infected by the pseudogramineous meloidogyne can be reduced; the method has potential and practical application value in research of the Meloidogyne pseudoaceae.

The invention belongs to the technical field of gene engineering, and particularly relates to a 3-ketosteroid-delta1-dehydrogenase mutant with improved heat resistance and an application of the 3-ketosteroid-delta1-dehydrogenase mutant. The 3-ketosterone-delta1-dehydrogenase AuKsdD gene derived from arthrobacter urate oxydans is subjected to structural analysis and site-directed mutagenesis design, and a mutant with higher catalytic activity and remarkably enhanced heat resistance compared with a wild type AuKsdD is obtained through directed mutation of key sites E244, K267, T301 and A335. The mutant can keep high catalytic efficiency for a long time under a high-temperature condition, and is suitable for industrial synthesis of steroid drug raw materials.

The application provides a strain of Arthrobacter sp. ZJUHUYJH1 and application thereof in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of prevention and treatment of plant root-knot nematodes. The Arthrobacter sp. ZJUHUYJH1 provided by the application has a preservation number of CCTCC NO: M 2025922, is isolated from rice rhizosphere soil infected by paratyphaceae root-knot nematodes, has a good prevention and treatment effect on paratyphaceae root-knot nematodes, a rectification mortality of 91.4% on second instar larvae of root-knot nematodes, can reduce the number of root-knots and the ratio of the number of root-knots to dry weight of rice infected by paratyphaceae root-knot nematodes, and has potential and practical application value in the research of paratyphaceae root-knot nematodes.

The application discloses a kind of based on whole solid waste carbon reduction utilization's goaf stable filling system and filling method, the system includes biological preparation culture system, solid waste prefabrication system, gas injection system and goaf, the biological preparation culture system includes two incubators, and solid waste prefabrication system includes two prefabricators.Acid bacillus, arthrobacter are respectively added into two incubators and are respectively entered into two prefabricators after cultivation, steel slag is added into the prefabricator of acid bacillus bacterial solution, calcium carbide slag, granulated blast furnace slag are added into the prefabricator of arthrobacter bacterial solution, obtain two kinds of solid waste-biological bacterial solution mixture injection mineral resources goaf, pass in carbon dioxide-air mixed gas reaction, complete the stable filling of goaf.The present application is glued into the filling body with certain strength by microorganism carbon fixation mineralization reaction and solid waste pozzolanic reaction, not only effectively utilizes greenhouse gas carbon dioxide and industrial waste, but also realizes the safe treatment of mineral resources goaf.

The invention discloses ammonia assimilation arthrobacter arylalani and application of the ammonia assimilation arthrobacter arylalani in aquaculture water treatment, and relates to the technical field of wastewater treatment, the ammonia assimilation arthrobacter arylalani is arthrobacter arylalani Aa01 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36829, and the preservation date is December 01, 2025. The invention also discloses application of the ammonia assimilation arthrobacter ashlii in aquaculture water treatment. The ammonia assimilation arthrobacter arabilis Aa01 provided by the invention is a microbial strain with remarkable characteristics and application value, the strain has the most remarkable characteristic of a unique nitrogen conversion mechanism, NH4 < + >-N, NO2 <->-N and NO3 <->-N can be efficiently used as electron acceptors for respiration under an aerobic condition, and the dependence of a traditional denitrification process on an anaerobic environment is broken through; compared with a conventional biological denitrification strain, the Aa01 strain shows excellent performance advantages.

The application discloses a microbial agent for improving cotton yield and quality and a preparation method thereof, and belongs to the technical field of organic fertilizer and microbial fertilizer manufacturing. The microbial agent for improving cotton yield and quality comprises the following raw materials in parts by weight: 50-60 parts of a compound microbial liquid, 8-10 parts of alginate oligosaccharide, 6-8 parts of humic acid chelated boron zinc, 4-5 parts of polyglutamic acid, 12-15 parts of modified attapulgite, and 2-3 parts of glycerol; the compound microbial liquid comprises Candida valensis, Arthrobacter mysorens, and Pseudomonas psychrophilus. The three strains are colonized in the rhizosphere of cotton together, synergistically promote the growth of cotton, make cotton seeds full, increase the yield of cotton, effectively improve the quality of lint, realize the dual targets of yield increase and quality improvement, reduce the use of chemical fertilizers and pesticides, and are a very promising technical scheme for promoting green, high-yield, high-quality and efficient production of cotton.

This invention provides a group of microbial flora for the degradation of short-chain phthalates and the repair of plant damage, belonging to the field of microbial technology. The aforementioned microbial flora includes *Arthrobacter* (…). Paenarthrobacter sp.) PAEs3-4, Corynebacterium glutamicum (sp.) Glutamicibacter sp.) A4 and Pseudomonas aeruginosa (sp.) A4 and Pseudomonas aeruginosa ( Pseudomonas Paenarthrobacter sp. 60231 and Paenarthrobacter sp. PAEs3-4 were deposited at the China Center for Type Culture Collection (CCTCC) on January 16, 2026, with accession number CCTCC M 2026142. These bacterial groups can achieve plant damage repair during growth while ensuring the degradation of short-chain PAEs.

This invention belongs to the field of microbial technology and relates to a salt-tolerant Arthrobacter strain, its microecological preparations, and applications. The invention uses a salt-tolerant Arthrobacter strain derived from the rhizosphere of aquatic plants as the starting strain. Through ultraviolet mutagenesis, a new strain producing high levels of indoleacetic acid was obtained. This strain has been deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number: CGMCC No. 30670. This bacterium possesses the ability to produce high levels of indoleacetic acid and can promote the growth of aquatic plants.

The invention relates to a complex microbial inoculant for preventing and treating tobacco bacterial wilt and application of the complex microbial inoculant. Strain in the complex microbial inoculant comprises a mulberry enterobacter 65D6 strain, a Ralstonia 56D2 strain, an agrobacterium 18A6 strain, an arthrobacter uretogenes 05-507 strain and a bacillus safensis 05-2101 strain. The five specific strains are compounded and combined for use, an excellent tobacco bacterial wilt prevention and treatment effect is achieved, and experimental research shows that the disease occurrence of tobacco plants treated by the complex microbial inoculant is obviously slowed down, and the disease index is obviously lower than that of a clear water control group and that of a thiediazole copper and chloriure.copper sulfate chemical agent control group. Meanwhile, the five strains can be mutually matched and promoted, a synergistic effect is achieved in the aspect of preventing and treating tobacco bacterial wilt, the WY11 strain is further added for combined use, the effect of preventing and treating the tobacco bacterial wilt is improved, and the better effect of promoting the growth of tobacco plants can be achieved.

This invention relates to the field of ecological agriculture technology, specifically disclosing a segmented erosion control method for sloping farmland based on plant hedges. The method includes the following steps: dividing the sloping farmland into upper, middle, and lower planting areas according to the terrain from high to low; and then correspondingly installing upper, middle, and lower planting hedges, and constructing a plant community in the lower area. The installation of upper planting hedges includes inoculating the rhizosphere soil with nematodes, or with a combination of nematodes and microbial agents. The microbial agents include at least one of Bacillus, Arthrobacter, Neurospora, or Penicillium. This invention integrates "source control, reservoir enhancement, and potential tapping," effectively intercepting surface runoff and increasing soil moisture infiltration by altering the slope's micro-topography. This is of great significance for improving soil fertility and water and fertilizer resource utilization in dryland farmland, reducing fertilizer use and nitrogen and phosphorus loss, balancing nutrient distribution on the slope, increasing nutrient utilization, and ensuring ecological security.

This invention discloses a plant rhizosphere growth-promoting bacterium, *Aristolochia nitroguaiacos* (…). Paenarthrobacter nitroguajacolicus YD-2-4, deposited at the China Microbial Culture Collection Center (CGMCC) under accession number CGMCC No. 36649, possesses various growth-promoting properties, including nitrogen fixation, phosphorus solubilization, IAA production, and ACC deaminase production. It significantly promotes the growth of forage grasses such as alfalfa, wheatgrass, crested wheatgrass, red fescue, Artemisia arenaria, and Stipa purpurea. The strain promoted shoot growth by 25.98% and 14.85% in Artemisia arenaria and red fescue, respectively, and root growth by 27.98% and 19.85% in Artemisia arenaria and Stipa purpurea, respectively. It also increased biomass accumulation in Artemisia arenaria and red fescue by 36.60% and 22.91%, respectively. Regarding plant antioxidant capacity, YD-2-4 significantly enhanced peroxidase activity in Artemisia arenaria and alfalfa, and increased the total antioxidant capacity of red fescue and Stipa purpurea. Strain YD-2-4 can serve as an excellent plant rhizosphere growth promoter, and has broad application prospects in the microbial remediation of degraded grasslands in alpine regions and the development of biofertilizers.

The present application relates to the technical field of bioengineering, and particularly relates to a kind of omega-transaminase mutant and its application.The present application discloses a kind of omega-transaminase mutant and its application in the synthesis of silodosin intermediate, the omega-transaminase mutant is by from Arthrobacter, through homologous modeling, reaction intermediate molecular docking, active site amino acid analysis, homologous sequence analysis, virtual mutation analysis combined with site-directed mutation verification, the single-point mutation of 62th and 223th obtains H62A, S223A mutant.The omega-transaminase mutant of the present application can one-pot catalysis 60mM silodosin intermediate ketone is converted into silodosin intermediate, conversion rate is greater than 92%, compared with wild type, the specific activity of H62A mutant to silodosin intermediate ketone is 1.4 times of wild type, S223A is 1.24 times of wild type, and the optimum reaction temperature is 30 DEG C, and the ee value of product is greater than 99.29%.

The application discloses a kind of for livestock and poultry manure quick rotting complex microbial inoculant and its preparation method and application, belong to microbial technical field.The complex microbial inoculant of the application includes complex microbial fermentation liquor 30-40 parts, organic carrier and conditioner 40-50 parts, nutrient adjuvant 5-8 parts, mineral element containing adjuvant 3-5 parts, accelerator 2-4 parts, wherein complex microbial fermentation liquor includes thermophilic Bacillus, high-temperature-resistant Bacillus heterosporus, nourishing arthrobacter.The three strains of the complex microbial inoculant of the application are selected, and the synergistic effect is remarkable, thermophilic Bacillus and high-temperature-resistant Bacillus heterosporus dominate high-temperature stage, rapidly decompose various organic matters and kill pathogenic bacteria and insect eggs;Nourishing arthrobacter dominates medium-low temperature stage, thoroughly deodorizes and completes humification.Three synergies realize the whole cycle coverage of sheep manure rotting process, and the rotting effect is good, and the rotting material is loose, odorless, and the seed germination index is high.

The invention discloses a multi-effect synthetic flora microbial agent as well as a preparation method and application thereof. The multi-effect synthetic flora microbial agent is prepared from the following main components: enterobacter sphenodendron G-N-2, bacillus subtilis RZ-N-1, bacillus subtilis RZ-IP-4, acinetobacter calcoaceticus RZ-K-1, arthrobacter crystalliforme RZ-CAS-4, simple pure bacillus Y-CAS-3, bacillus J-IAA-18 and bacillus tequilensis G-IAA-1, and has a promoting effect on the growth of plants on the degenerated sand land; wherein the plant biomass can be increased by 24.2% when the multi-effect synthetic flora microbial agent is applied to the seriously degraded sand land.

The invention discloses arthrobacter for improving acidic white pulp soil and a microbial agent and application of the arthrobacter. The Arthrobacter sp. W36-1 is preserved in CGMCC (China General Microbiological Culture Collection Center), and the preservation number of the Arthrobacter sp. W36-1 is No. 31903. The bacterial strain is separated from white pulp soil, and is characterized by having efficient biological acid reduction (alkali production) capability, and a culture medium with the pH value of 5.0 can be increased to 7.66 within 72 hours. Under the acid stress condition that the pH value is less than or equal to 5.5, the strain can synchronously play multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing IAA and EPS and the like. Pot experiments prove that the microbial inoculum can effectively improve the pH value of acidic white slurry soil, remarkably relieve damage of acid stress to soybeans, promote root growth of the soybeans, increase the number of root nodules and biomass and have a good growth promoting effect on rice germination under acid stress.

The invention relates to the technical field of microorganisms, and particularly discloses arthrobacter Y37 as well as a fungicide and application thereof, the arthrobacter Y37 is preserved in China General Microbiological Culture Collection Center on April 15, 2025, the preservation number is CGMCC No.34220, and the arthrobacter Y37 is classified and named as arthrobacter sp. The arthrobacter Y37 provided by the invention is used for promoting the growth of cherry plants and the germination of apple seeds.

The invention discloses a microbial agent for improving the yield and quality of cotton and a preparation method thereof, and belongs to the technical field of organic fertilizer and microbial fertilizer manufacturing. The microbial agent for improving the cotton yield and quality is prepared from the following raw materials in parts by weight: 50 to 60 parts of compound microbial liquid, 8 to 10 parts of alginate oligosaccharide, 6 to 8 parts of humic acid chelated boron zinc, 4 to 5 parts of polyglutamic acid, 12 to 15 parts of modified attapulgite and 2 to 3 parts of glycerol, the compound microorganism bacterial liquid is prepared from candida albicans, arthrobacter michaeli and cold-resistant pseudomonas. The three strains are colonized at the rhizosphere of the cotton together, have synergistic interaction, can promote the growth of the cotton in all directions, enable the cotton seeds to be full, improve the yield, effectively improve the quality of the ginned cotton, achieve the dual goals of increasing the yield and improving the quality, reduce the use of chemical fertilizers and pesticides, and reduce the production cost. The method is a promising technical scheme for promoting green, high-yield, high-quality and high-efficiency production of cotton.

The application belongs to the technical field of biology and relates to an enzyme protein, in particular to a recombinant Arthrobacter globiformis uricase and application thereof. The recombinant Arthrobacter globiformis uricase has a single-point amino acid mutation, and the amino acid is located in an active region and a non-active region of the uricase, and the amino acid sequence of the recombinant Arthrobacter globiformis uricase is shown in any one of SEQ ID NO: 1-8. The mutant uricase formed after the amino acid is introduced into the uricase has higher activity than the wild-type uricase, and in the case of effectively controlling the uric acid content, the use amount of the uricase can be more effectively reduced.

The application discloses Arthrobacter NJAU-OF121, which is classified and named as Arthrobacter nitrophenolicus and has a preservation number of CGMCC NO.34968. The application also discloses an acid-resistant aluminum growth-promoting biochar-based microbial agent, and a preparation method and application thereof. The Arthrobacter NJAU-OF121 can grow under acidic conditions (pH 4.5) and has the performances of IAA production, phosphorus dissolution and aluminum toxicity resistance under acidic conditions (pH 4.5), thereby providing a new strain resource for the production capacity improvement of acidic soil. In the acid-resistant aluminum growth-promoting biochar-based microbial agent, the survival number of functional bacteria is significantly improved, and the agent has more excellent effects of promoting the growth of rape (including relieving the aluminum toxicity and phosphorus deficiency of rape) and increasing yield (rape seed yield), thereby providing an effective guarantee for the high-yield cultivation of rape on acidic soil.

This invention discloses a strain of *Arthrobacter* (…). Pseudarthrobacter The strain F13, with accession number CCTCC NO:M 20241866 at the China Center for Type Culture Collection, was used in this invention to degrade procymidone. The results showed that the *Pseudomonas* F13 strain of this invention can efficiently degrade procymidone. This invention enriches the germplasm resources and diversity of microbial degrading strains and provides a new approach for the control of pesticide residue pollution.