19 results about "Arthrobacter" patented technology

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The invention relates to a preparation method of prednisone acetate. The preparation method comprises the following steps: carrying out secondary culture on arthrobacter simplex; adding a crude product of cortisone acetate into the arthrobacter simplex subjected to secondary culture, performing fermentation conversion, and performing conversion by adopting a mode of gradient temperature control and batch addition of an organic solvent to obtain a crude product of prednisone acetate; refining the prednisone acetate crude product, pulping with ethyl acetate, heating and refluxing, and repeatedly refining for multiple times to obtain a prednisone acetate refined product; and heating and dissolving the prednisone acetate refined product with ethyl acetate, adding activated carbon and water, heating and refluxing, filtering and concentrating to obtain the prednisone acetate refined product. According to the method disclosed by the invention, a mode of gradient temperature control and batch addition of the organic solvent is adopted during conversion, so that the strain conversion efficiency is improved, and the conversion rate of prednisone acetate reaches 94% or above; during refining, a pulping mode is adopted, so that the batch feeding amount is increased, a repeated refining and concentrating process is removed, the solvent consumption is reduced, and the process time is greatly shortened.

The invention provides an arthrobotrys oligospora genetic engineering strain with nematode attracting activity, and belongs to the technical field of microbial genetic engineering, the genetic engineering strain takes arthrobotrys oligospora as a starting strain, and the genetic engineering strain is a strain obtained by knocking out an AoETF beta gene in an arthrobotrys oligospora genome. The arthrobotrys oligospora genetic engineering strain has nematode attraction activity, and can attract nematodes in soil to gather together so as to achieve the effect of efficiently capturing and killing the nematodes, thereby providing a basis for biological control of the nematodes. The invention further provides a construction method and application of the arthrobotrys oligospora genetic engineering strain with nematode attracting activity.

The application belongs to the field of microorganisms and the field of environmental protection, and provides at least one urea-producing Paenarthrobacter ureafaciens for degrading atrazine, wherein the urea-producing Paenarthrobacter ureafaciens is preserved in the China General Microbiological Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, located at No. 1, Xiliujia, Beijing Chaoyang District, and has the preservation numbers of CGMCC No. 37944 or CGMCC No. 37943, respectively. The application obtains the urea-producing Paenarthrobacter ureafaciens with high efficiency, wide spectrum and strong environmental adaptability through screening, and finally realizes rapid and complete repair of atrazine contaminated soil by matching an optimized microbial agent formula and a preparation process, so as to reduce repair cost, promote the large-scale application of the microbial repair technology in farmland and industrial pollution sites, and solve many technical bottlenecks in the field of atrazine pollution repair.

This invention relates to Nicotine-like Arthrobacter or contain Nicotine Arthroblasts The use of bacterial combinations for the degradation of one or more alkaloids in processed tobacco.

The invention discloses a degrading bacterium system for repairing pesticide-polluted soil and application of the degrading bacterium system. The degrading bacterial system is composed of Pseudomonas plecoglossa JY-5, Klebsiella plecoglossa J2-1, Arthrobacter silvisoli JY-2 and Enterobacter ludwigii JY-1, and the degrading bacterial system is obtained by carrying out enrichment culture, screening, sequencing analysis and bacterial strain identification and purification on the soil polluted by the pesticide, and the degrading bacterial system is used for degrading the soil polluted by the pesticide, and the degrading bacterial system is used for degrading the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide and the soil polluted by the pesticide. According to the degrading bacterium system, the biochar is used as a carrier to prepare an immobilized bacterium agent, various common pesticides such as carbendazim and the like can be efficiently degraded, the strains have a synergistic effect, the stability in a complex soil environment is high, the anti-interference capability is outstanding, soil pesticide residues can be effectively reduced, an efficient and feasible technical scheme is provided for soil remediation in a pesticide high-pollution area, and the degrading bacterium system has wide application prospects. The method has important practical application value.

This invention provides an acid- and alkali-producing bacterial combination, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a bacterial combination including Bacillus subtilis with accession number CGMCC No. 29201. Bacillus sp S1 and Arthrobacterium with accession number CGMCC No. 29202 ( Arthrobacter sp S2. In this invention, both Bacillus S1 and Arthrobacter S2 are acid- and alkali-producing strains with strong environmental adaptability. Through synergistic effects via multiple pathways, including ammonia production, bicarbonate secretion, and activation of soil enzymes, the bacterial combination can significantly increase soil pH, reduce exchangeable aluminum and hydrolyzable acids, and simultaneously increase soil bicarbonate content, thus rapidly and comprehensively improving soil acidity from its root cause. This bacterial combination is of great significance for achieving green, efficient, and sustainable remediation of acidified soils.

The invention belongs to the technical field of gene engineering, and particularly relates to a 3-ketosteroid-delta1-dehydrogenase mutant with improved heat resistance and an application of the 3-ketosteroid-delta1-dehydrogenase mutant. The 3-ketosterone-delta1-dehydrogenase AuKsdD gene derived from arthrobacter urate oxydans is subjected to structural analysis and site-directed mutagenesis design, and a mutant with higher catalytic activity and remarkably enhanced heat resistance compared with a wild type AuKsdD is obtained through directed mutation of key sites E244, K267, T301 and A335. The mutant can keep high catalytic efficiency for a long time under a high-temperature condition, and is suitable for industrial synthesis of steroid drug raw materials.

The application provides a strain of Arthrobacter sp. ZJUHUYJH1 and application thereof in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of prevention and treatment of plant root-knot nematodes. The Arthrobacter sp. ZJUHUYJH1 provided by the application has a preservation number of CCTCC NO: M 2025922, is isolated from rice rhizosphere soil infected by paratyphaceae root-knot nematodes, has a good prevention and treatment effect on paratyphaceae root-knot nematodes, a rectification mortality of 91.4% on second instar larvae of root-knot nematodes, can reduce the number of root-knots and the ratio of the number of root-knots to dry weight of rice infected by paratyphaceae root-knot nematodes, and has potential and practical application value in the research of paratyphaceae root-knot nematodes.

The invention discloses ammonia assimilation arthrobacter arylalani and application of the ammonia assimilation arthrobacter arylalani in aquaculture water treatment, and relates to the technical field of wastewater treatment, the ammonia assimilation arthrobacter arylalani is arthrobacter arylalani Aa01 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36829, and the preservation date is December 01, 2025. The invention also discloses application of the ammonia assimilation arthrobacter ashlii in aquaculture water treatment. The ammonia assimilation arthrobacter arabilis Aa01 provided by the invention is a microbial strain with remarkable characteristics and application value, the strain has the most remarkable characteristic of a unique nitrogen conversion mechanism, NH4 < + >-N, NO2 <->-N and NO3 <->-N can be efficiently used as electron acceptors for respiration under an aerobic condition, and the dependence of a traditional denitrification process on an anaerobic environment is broken through; compared with a conventional biological denitrification strain, the Aa01 strain shows excellent performance advantages.

This invention provides a group of microbial flora for the degradation of short-chain phthalates and the repair of plant damage, belonging to the field of microbial technology. The aforementioned microbial flora includes *Arthrobacter* (…). Paenarthrobacter sp.) PAEs3-4, Corynebacterium glutamicum (sp.) Glutamicibacter sp.) A4 and Pseudomonas aeruginosa (sp.) A4 and Pseudomonas aeruginosa ( Pseudomonas Paenarthrobacter sp. 60231 and Paenarthrobacter sp. PAEs3-4 were deposited at the China Center for Type Culture Collection (CCTCC) on January 16, 2026, with accession number CCTCC M 2026142. These bacterial groups can achieve plant damage repair during growth while ensuring the degradation of short-chain PAEs.

This invention relates to the field of ecological agriculture technology, specifically disclosing a segmented erosion control method for sloping farmland based on plant hedges. The method includes the following steps: dividing the sloping farmland into upper, middle, and lower planting areas according to the terrain from high to low; and then correspondingly installing upper, middle, and lower planting hedges, and constructing a plant community in the lower area. The installation of upper planting hedges includes inoculating the rhizosphere soil with nematodes, or with a combination of nematodes and microbial agents. The microbial agents include at least one of Bacillus, Arthrobacter, Neurospora, or Penicillium. This invention integrates "source control, reservoir enhancement, and potential tapping," effectively intercepting surface runoff and increasing soil moisture infiltration by altering the slope's micro-topography. This is of great significance for improving soil fertility and water and fertilizer resource utilization in dryland farmland, reducing fertilizer use and nitrogen and phosphorus loss, balancing nutrient distribution on the slope, increasing nutrient utilization, and ensuring ecological security.

This invention discloses a plant rhizosphere growth-promoting bacterium, *Aristolochia nitroguaiacos* (…). Paenarthrobacter nitroguajacolicus YD-2-4, deposited at the China Microbial Culture Collection Center (CGMCC) under accession number CGMCC No. 36649, possesses various growth-promoting properties, including nitrogen fixation, phosphorus solubilization, IAA production, and ACC deaminase production. It significantly promotes the growth of forage grasses such as alfalfa, wheatgrass, crested wheatgrass, red fescue, Artemisia arenaria, and Stipa purpurea. The strain promoted shoot growth by 25.98% and 14.85% in Artemisia arenaria and red fescue, respectively, and root growth by 27.98% and 19.85% in Artemisia arenaria and Stipa purpurea, respectively. It also increased biomass accumulation in Artemisia arenaria and red fescue by 36.60% and 22.91%, respectively. Regarding plant antioxidant capacity, YD-2-4 significantly enhanced peroxidase activity in Artemisia arenaria and alfalfa, and increased the total antioxidant capacity of red fescue and Stipa purpurea. Strain YD-2-4 can serve as an excellent plant rhizosphere growth promoter, and has broad application prospects in the microbial remediation of degraded grasslands in alpine regions and the development of biofertilizers.

The application discloses a kind of for livestock and poultry manure quick rotting complex microbial inoculant and its preparation method and application, belong to microbial technical field.The complex microbial inoculant of the application includes complex microbial fermentation liquor 30-40 parts, organic carrier and conditioner 40-50 parts, nutrient adjuvant 5-8 parts, mineral element containing adjuvant 3-5 parts, accelerator 2-4 parts, wherein complex microbial fermentation liquor includes thermophilic Bacillus, high-temperature-resistant Bacillus heterosporus, nourishing arthrobacter.The three strains of the complex microbial inoculant of the application are selected, and the synergistic effect is remarkable, thermophilic Bacillus and high-temperature-resistant Bacillus heterosporus dominate high-temperature stage, rapidly decompose various organic matters and kill pathogenic bacteria and insect eggs;Nourishing arthrobacter dominates medium-low temperature stage, thoroughly deodorizes and completes humification.Three synergies realize the whole cycle coverage of sheep manure rotting process, and the rotting effect is good, and the rotting material is loose, odorless, and the seed germination index is high.

The invention discloses arthrobacter for improving acidic white pulp soil and a microbial agent and application of the arthrobacter. The Arthrobacter sp. W36-1 is preserved in CGMCC (China General Microbiological Culture Collection Center), and the preservation number of the Arthrobacter sp. W36-1 is No. 31903. The bacterial strain is separated from white pulp soil, and is characterized by having efficient biological acid reduction (alkali production) capability, and a culture medium with the pH value of 5.0 can be increased to 7.66 within 72 hours. Under the acid stress condition that the pH value is less than or equal to 5.5, the strain can synchronously play multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing IAA and EPS and the like. Pot experiments prove that the microbial inoculum can effectively improve the pH value of acidic white slurry soil, remarkably relieve damage of acid stress to soybeans, promote root growth of the soybeans, increase the number of root nodules and biomass and have a good growth promoting effect on rice germination under acid stress.

The application belongs to the technical field of biology and relates to an enzyme protein, in particular to a recombinant Arthrobacter globiformis uricase and application thereof. The recombinant Arthrobacter globiformis uricase has a single-point amino acid mutation, and the amino acid is located in an active region and a non-active region of the uricase, and the amino acid sequence of the recombinant Arthrobacter globiformis uricase is shown in any one of SEQ ID NO: 1-8. The mutant uricase formed after the amino acid is introduced into the uricase has higher activity than the wild-type uricase, and in the case of effectively controlling the uric acid content, the use amount of the uricase can be more effectively reduced.

The application discloses Arthrobacter NJAU-OF121, which is classified and named as Arthrobacter nitrophenolicus and has a preservation number of CGMCC NO.34968. The application also discloses an acid-resistant aluminum growth-promoting biochar-based microbial agent, and a preparation method and application thereof. The Arthrobacter NJAU-OF121 can grow under acidic conditions (pH 4.5) and has the performances of IAA production, phosphorus dissolution and aluminum toxicity resistance under acidic conditions (pH 4.5), thereby providing a new strain resource for the production capacity improvement of acidic soil. In the acid-resistant aluminum growth-promoting biochar-based microbial agent, the survival number of functional bacteria is significantly improved, and the agent has more excellent effects of promoting the growth of rape (including relieving the aluminum toxicity and phosphorus deficiency of rape) and increasing yield (rape seed yield), thereby providing an effective guarantee for the high-yield cultivation of rape on acidic soil.

The invention relates to the field of soil remediation, and discloses an in-situ synergistic remediation method for atrazine-contaminated soil, arthrobacter uretogenus ATZ-1 and application of the arthrobacter uretogenus ATZ-1. According to the method, animal waste and straw are used as substrates for composting, wine grape skin residues are added in the composting process to serve as humification promoters to improve the humification degree, and reinforced compost is obtained; the reinforced compost and the arthrobacter uretogenus ATZ-1 are jointly applied to the atrazine-polluted soil, and the atrazine-polluted soil is maintained under preset conditions. The enhanced compost as an ecological carrier can improve the survival / colonization and metabolic activity of ATZ-1 in soil, so that atrazine is efficiently removed in a short period, and the improvement of soil biochemical activity (urease and catalase) and the enhancement of the robustness of a microorganism interaction network are considered.

This invention provides a Burkholderia spp. bacterium, a synthetic bacterial community, and their application for the control of white rot disease in Coptis chinensis, belonging to the field of microbial technology. The Burkholderia spp. bacterium HJ23 has the preservation number CCTCC NO: M 20253076. This invention provides a synthetic bacterial community including Burkholderia spp. bacterium HJ23, Pseudomonas fluorescens, and Arthrobacter spp. This synthetic bacterial community can more effectively inhibit the growth of the pathogen causing white rot disease, controlling white rot in Coptis chinensis and peanuts. This synthetic bacterial community provides a novel and highly effective antagonistic strain resource for the green biological control of this soil-borne disease, and can effectively replace or reduce the use of chemical pesticides, reducing the risk of pesticide residues. This aligns with the development needs of green cultivation of Chinese medicinal herbs, and has significant practical value for ensuring the quality of Coptis chinensis, improving the economic benefits of Coptis chinensis cultivation, and promoting the sustainable development of the Coptis chinensis cultivation industry.