42 results about "Perinereis aibuhitensis" patented technology

The invention discloses a method for the three-dimensional and artificial seedlings cultivation of the perinereis aibuhitensis, which mainly comprises the steps of: (1) fishing and disinfecting the heteronereis; (2) laying eggs, hatching and cultivating the planktonic larva; (3) three-dimensionally cultivating the benthic larvae; and (4) seedling emerging. The key measures comprise the steps of: putting four setiger intermediate-later stage nectochaete into a polyethylene water tank for the three-dimensional cultivation, cultivating by flowing water, and properly mixing the single-cell algae, the EM (effective microorganisms) bacterium with various artificial baits. After the measures are used, the density of the larvae is reduced, the mutual massacre among the individuals is reduced, the nutritional requirement of each-stage larva and the good and stable water quality environment are guaranteed, the survival rate of the larva is greatly improved, the breeding rate from the fertilized ovum to the sand silkworm seedling with the length of 1cm reaches at 42.3%, and the seedling yield per unit area is improved at 11.4*104 numbers/m<2>, so that the survival rate, the breeding rate and the seedling yield per unit area are greatly improved compared with the existing seedlings cultivation mode. The method is convenient to operate, can prevent the seedlings from being damaged caused by sand flushing and seedling driving, and is good for the long-distance transporting and the planting of the seedlings.

The present invention is one kind of protein extracted from Perinereis aibuhiteris and its preparation process and use. The protein is alkali protein with molecular weight 65000 Da and isoelectric point of 8.3. The protein preparing process includes raising Perinereis aibuhiteris in artificial sea water for 24-48 hr to make it become clean, electrically striking Perinereis aibuhiteris with 5-10 V DC to obtain body fluid, centrifugally filtering body fluid, reverse concentration, gel filtering, and chromatographic elution in anionic exchange column to collect active protein. The protein is used in preparing antibiotic medicine resisting escherichia coli, verdigris pseudomonad, staphylococcus aureus and arthrobacter and anticancer medicine for suppressing human liver cancer cell strain proliferation and producing alpha-fetoglobulin, and has high antibiotic activity and obvious anticancer effect.

Disclosed is a perinereis aibuhitensis totally-artificial breeding technology. The technical processes of the perinereis aibuhitensis totally-artificial breeding technology include the steps of parent perinereis aibuhitensis selection, indoor cement pit culturing, daily management, sexual maturity, artificial regulation for breeding, centralized hatching, young perinereis aibuhitensis breeding, soil pool breeding, culturing management and harvesting. Through research on perinereis aibuhitensis domestication breeding, artificial parent perinereis aibuhitensis cultivation, the artificial insemination method, young perinereis aibuhitensis production and large-scale cultivation, the perinereis aibuhitensis artificial cultivation and artificial breeding technologies and the perinereis aibuhitensis production processes are formed, the problems that young perinereis aibuhitensis supply is not enough and limited by seasons are solved, perinereis aibuhitensis fertilization rates, hatchability which is about 60% and young perinereis aibuhitensis survival rates are improved, and guarantees in quality and quantity are provided for artificial breeding in the future; furthermore, the perinereis aibuhitensis totally-artificial breeding technology has significance in conducting large-scale perinereis aibuhitensis cultivation, improving cultivation structure adjustment, increasing income of fishermen and guaranteeing the sustainable development of the marine aquaculture industry.

A preparing method of a perinereis aibuhitensis anticoagulation peptide is disclosed. The method includes steps of (1) sample pretreatment, (2) enzymatic hydrolysis, (3) ultrafiltration, (4) anion exchange chromatography, (5) dextran gel G-25 chromatography, and (6) reversed-phase high-performance liquid chromatography separation and purification to finally obtain the anticoagulation peptide. The method has characteristics of high safety, mild reaction conditions and easy process control. The prepared anticoagulation peptide has high purity, high anticoagulation activity, an antithrombotic function and good medical application value.

The invention discloses an indoor intensive production method of perinereis aibuhitensis seeds, belonging to the field of aquaculture. The method comprises the steps of preparing a cement pond, preparing mud for culturing, preparing perinereis aibuhitensis culture boxes, distributing larva seeds, breeding the seeds, daily managing and enabling the seeds to emerge. In the cement pond, the multiple layers of small perinereis aibuhitensis culture boxes are arranged in a staggered stacking way, the attachment surface area of larvae in the cement pond can be increased, indoor intensive high-density production of the perinereis aibuhitensis seeds is realized, the seed output of the cement pond of a unit water body is increased, outdoor earth pond culture is not needed, the seeds with the large size of 20 to 30mm are directly obtained, and large-scale intensive production of the seeds is realized; if the perinereis aibuhitensis seeds are sold or broadcast remotely, the perinereis aibuhitensis seeds can be conveyed with the boxes and soil, convenience and quickness are realized, and damage caused in the collection, transfer and conveying processes of perinereis aibuhitensis larvae is avoided.

The invention relates to gene sequence and amino acid sequence of coding perinereis aibuhitensis grube cysteine protease inhibitor and insect-resistant activity of recombinant protein produced by the gene. The gene sequence and the amino acid sequence of the code protein are respectively shown like sequence 1 and sequence 2 in the chart, wherein the length of nucleotide sequence of the gene of coding perinereis aibuhitensis grube cysteine protease inhibitor is 333bp, the total length of the amino acid sequence of coding perinereis aibuhitensis grube cysteine protease inhibitor includes 110 amino acid residues, and recombinant cysteine protease inhibitor is clearly characterized by insect-resistant activity, which belongs to the field of agricultural biotechnology. Based on the gene of clamworm cysteine protease inhibitor of the invention, the coding gene of the cysteine protease inhibitor can be cloned from the cDNA library of the perinereis aibuhitensis grube. The gene of the cysteine protease inhibitor can be introduced into the plant to cultivate anti-insect plants by gene recombination technique. The gene production has recombinant clamworm cysteine protease inhibitor with insect-resistant activity, which can also be showed in other cells. The recombinant protein can be considered as novel biological pesticide to protect the agriculture from pest hazard.

The present invention discloses Perinereis aibuhitensis plasmin, which contains amino acid sequences represented by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4, has the molecular weight of 33.03 kDa, has the isoelectric point of 5.28, and has the fibrinolytic activity of 10000+/-1000 U/mg. The invention further provides a preparation method of the Perinereis aibuhitensis plasmin, wherein the preparation method comprises: pre-treating Perinereis aibuhitensis, carrying out gel column chromatography on crude Perinereis aibuhitensis enzyme, and then sequentially carrying out gel filtration, ultra-filtration desalination, ion exchange column chromatography and electrophoresis preparation so as to obtain the Perinereis aibuhitensis plasmin having strong fibrinolytic activity, wherein the obtained Perinereis aibuhitensis plasmin can be used as the thrombotic disease treatment bulk drug. According to the present invention, the method has the simple operation and is controllable, and the Perinereis aibuhitensis plasmin with characteristics of high fibrinolytic activity and high purity can be prepared with the method of the present invention.

A control method for indoor early propagation of Perinereis aibuhitensis is characterized by including the sequential steps of 1, preparing a breeding cement pool; 2, selecting parents of Perinereis aibuhitensis; thirdly, managing ways of breeding outside cages and breeding density; 4, performing daily winter management; and 5, after temperature control, restoring natural water temperature, namely continuing temperature control 30-40 days after normal low-temperature period, and keeping the indoor temperature at about 25 DEG C so that mature heteronereis can be found in about one week. The method has the advantages the method is performed in indoor cement pools and insusceptible to seasons and tides; simultaneous propagation of a great amount of parents can be extended to mid-March, and juvenile cultivation can be arranged in the first half year designedly; through the control way of artificial heating, cultivation is 3 months ahead, and fertility rate and hatching rate are the same as those of Perinereis aibuhitensis calculated in normal seasons; and the method is suitable for industrial production of juvenile of Perinereis aibuhitensis.

The invention relates to a method for perinereis aibuhitensis heteronereis cultivation and synchronous collection and belongs to the technical field of aquaculture offspring seed breeding. The method is characterized by mainly comprising the steps of cultivation pool design, homo-nereis selection, breeding methods and management and heteronereis synchronous collection. Heteronereis cultivated by the method can all reach over 2.0g, not only is the brood amount improved, but also gametal quality is improved to a certain extent; simultaneously synchronous collection of the perinereis aibuhitensis heteronereis is achieved, the problem that part of heteronereis discharge sperm and lay eggs is solved, collection at night is replaced with collection in the daytie, and not only is heteronereis collection operation convenient, but also offspring seed breeding after the heteronereis collection operation is also greatly facilitated.

The invention discloses a method for breeding perinereis aibuhitensis grube and shellfishes by a tidal flat. The method comprises the following steps: (1) site selection: selecting a medium and high wattenschlick or silt tidal flat which has flat terrain and is rich in organic matters; (2) juvenile breeding: breeding and culturing to 13-15 setiger juveniles in a pond, transferring the setiger juveniles to the tidal flat for stocking, and throwing clam juveniles of which the lengths of shells are 1-2 cm; (3) breeding management: carrying out patrol inspection every day, entering a beach face toexamine growth conditions of clam worms when the beach is exposed, feeding the clam worms with special baits, neatening the beach surface, dredging a drainage ditch, removing crabs, snails, fishes and enemy organisms, completely draining accumulated water in hot days in summers, and frequently observing growth development conditions of the clam worm juveniles during management at ordinary times,wherein the special baits are prepared from the following components by mass: 20.0-30.0 parts of dehulled soybean meal, 10.0-20.0 parts of domestic fish meal, 5.0-10.0 parts of passion fruit residues,0.5-1.0 part of 2,4-D, 0.5-1.0 part of multivitamins and 0.5-1.0 part of composite mineral salt; and (4) fishing and catching adults.

The invention discloses a cloning method of a perinereis aibuhitensis metal binding protein gene. According to the invention, a specific segment amplification method and a RACE amplification method are designed, and a perinereis aibuhitensis metal binding protein gene is cloned from perinereis aibuhitensis body wall muscle total RNA. With the method, the MP II gene expression regularity of perinereis aibuhitensis suffered from stresses of pollutants such as heavy metals can be detected with a real-time fluorescence quantitative PCR technology. Therefore, the method provides a basis for the study of the mechanism of heavy metal regulation on perinereis aibuhitensis MP II gene. Also, the method assists in establishing a basis for screening molecular biomarkers for marine sedimentary environment pollution early-stage ecological risk forecasting. With the method, perinereis aibuhitensis with a low cost and an abundant source can be widely applied in environment protection.

The invention relates to perinereis aibuhitensis grube fermented feed and a preparation method thereof and belongs to the field of feed. The feed is prepared from, 30-35 parts of bean pulp, 10-15 parts of cottonseed meal, 5-10 parts of rapeseed meal, 5-10 parts of peanut meal, 25-35 parts of wheat, 0.3-0.5 part of mineral substance premix, 0.3-0.5 part of vitamin premix and 5-9 parts of cottonseed oil. After all the raw materials except for the cottonseed oil are mixed, the raw materials are fermented through bacillus subtilis, lactobacillus plantarum and clostridium butyricum, the cottonseed oil is added, and then the fermented feed is prepared. No animal protein raw material is used in the feed at all, so that production cost is reduced; the food calling effect and growth promoting effect of the fermented feed on perinereis aibuhitensis grube are good, digestive absorption is easy, the nutritional level and the conversion utilization rate of the feed are increased, the bait coefficient is reduced, and pollution to the breeding environment and the surrounding environment is reduced.

The invention discloses a coding cDNA sequence and amino acid sequence of coding perinereis aibuhitensis grube protease, as sequence 1 and sequence 2 of the sequence table show respectively, wherein, the full length of cDNA is 839bp, the length of coding nucleotide sequence of perinereis aibuhitensis grube protease is 765 bp, the full length of the coding amino acid sequence of perinereis aibuhitensis grube protease is 254 amino acid residues and the invention comprises 22 signal peptide composed by amino acid residues, which belongs to biomedicine field. According to the coding cDNA sequence of nereis protease of the invention, the encoding gene of protease can be cloned from cDNA library of the perinereis aibuhitensis grube, the encoding gene can be expressed in eukaryotic expression system and prokaryotic expression system by gene recombination technique. The recombined nereis protease is provided with original activated activity of fibrinferment, which can be used as thrombolytic drug to prevent or cure myocardial infarction, cerebral arterial thrombosis and the like.

The invention discloses a perinereis aibuhitensis anticoagulation peptide and use thereof. The amino acid sequence of the perinereis aibuhitensis anticoagulation peptide is Pro-Val-Glu-Arg-Lys. The perinereis aibuhitensis anticoagulation peptide is high in anticoagulation activity, has an antithrombus effect, and has a preferable medical application value.

The present invention provides human body sexual hormone improving health preserving food. The health preserving food is prepared from nereis succinea as a main material and prepared by compounding with sea cucumbers and fish oil, wherein the nereis succinea, sea cucumbers and fish oil have effects of mutual supports and synergistic interactions in functions. The health preserving food can safely and effectively promote human body secretion of sexual hormones, improves levels of the sexual hormones in the human body, enables eaters to restore sexual vitality and maintains reproductive health, youth and vitality. Perinereis aibuhitensis, especially obtained perinereis aibuhitensis in waters of Dongshan Island, Zhangzhou, Fujian, is preferred, and the health preserving food is prepared. The nereis succinea is rich in biosterins, amino acids, trace elements, active peptides, taurine, lumbrokinase, plasmin and other active substances, rich in nutrition, high in contents of active ingredients and free heavy metal pollution, so that the nereis succinea prepared health preserving food has advantages of being good in quality and more prominent in health preserving effects.

The invention relates to the technical field of cynoglossus semilaevis gunther breeding, in particular to a method for nutritionally regulating cynoglossus semilaevis gunther parent fish in the YellowRiver mouth to breed high-feminisation fry. The method comprise the steps of parental source and selection, parent fish breeding, artificial oxytocin insemination and hatching and fry culture. Accordingly, by optimizing the regulation mode, strengthening nutrition through perinereis aibuhitensis grube and regulating temperature for spawning and fertilization, the fertilization rate is 83.5%, thehatching rate is 74.6%, and the ratio of female to male is 5:2. The female rate problem of farmers is solved, the breeding cost of the farmers is greatly reduced, direct huge economic benefits are brought to the majority of the farmers, and development of the factory aquaculture industry of cynoglossus semilaevis gunther is further promoted.

The invention discloses a breeding method of perinereis aibuhitensis. The method includes the steps of (1) field selection; (2) larva breeding; (3) breeding management, wherein when perinereis aibuhitensis grows to 3-5 cm in length, perinereis aibuhitensis is fed with a special bait, seawater is put into a pool before feeding, after perinereis aibuhitensis is soaked in the pool for half an hour, the seawater is drained out to simulate a rising tide and a falling tide, then feeding is conducted, the simulation of the rising tide and the falling tide is carried out twice a day, and the special bait is prepared from, by mass, 20.0-30.0 parts of bean pulp without peels, 10.0-20.0 parts of domestic fish meal, 5.0-10.0 parts of siraitia grosvenorii extract dreg, 0.5-1 part of 2,4-dichlorophenoxyacetic acid, 0.5-1 part of compound vitamin and 0.5-1 part of compound mineral salt; (4) capture of adult perinereis aibuhitensis.

The invention discloses a method for breeding perinereis aibuhitensis grub and shellfish on ecological remediation mud flat. The method includes the steps: 1) site selection: selecting middle-high tidal mud or sediment mud flat with flat terrain and rich organic materials; 2) seedling breeding: culturing setiger juvenile with 13-15 joints in a pond, transplant and releasing the setiger juvenile onthe mud flat, and releasing clam seedlings with the shell length of 1-2cm; 3) aquaculture management: performing patrol inspection every day, entering a beach face to inspect the growth state of theperinereis aibuhitensis grub when a beach is exposed, feeding special baits, arranging the beach face, dredging drainage ditches, removing crabs, snails, fishes and harmful organisms, completely draining accumulated water under hot weather in summer, and frequently observing growth and development states of young perinereis aibuhitensis grub in management at ordinary times; 4) adult catching. Thespecial baits are prepared from, by weight, 20.0-30.0 parts of corn gluten meal, 10.0-20.0 parts of steam fish meal, 5.0-10.0 parts of fructus momordicae extract residues, 0.5-1.0 part of 2, 4-D, 0.5-1.0 part of multi-vitamins and 0.5-1.0 part of complex mineral salt.

The invention provides application of perinereis aibuhitensis grube in controlling petroleum hydrocarbon pollution and a control method, and relates to the technical field of petroleum hydrocarbon pollution remediation. The application comprises adding perinereis aibuhitensis grube into sediment of petroleum hydrocarbon pollution, wherein the addition density of the perinereis aibuhitensis grube is 300 to 400/m<2>. According to the application, when concentration of petroleum hydrocarbon in the sediment does not exceed 30 mg/g dry weight, in a process that the perinereis aibuhitensis grube isadded into the sediment of petroleum hydrocarbon pollution for 30d, the perinereis aibuhitensis grube performs normal burrowing and ingestion activies in the sediment, oxygen content of the sediment is increased, the adding of the perinereis aibuhitensis grube can effectively promote removal of the petroleum hydrocarbon in the sediment with a removal rate of 22% to 37%, and therefore, the application has characteristics of safety, efficiency, low cost and wide range of application.

The invention discloses a method for cultivating Perinereis aibuhitensis on a desolate beach and capable of reducing Spartina alterniflora. The method includes the steps of firstly, selecting a flat and medium or high tidal-mud or silt beach rich in organic matter to serve as the cultivation site; secondly, transferring young Perinereis aibuhitensis which is cultivated in a pond and has 16-18 setigers to the beach for stocking, and adding young clams with the shell length being 1-2cm; thirdly, performing cultivation management, to be more specific, performing patrol inspection every day, entering the beach to inspect the growth condition of the Perinereis aibuhitensis when the beach is exposed, feeding special bait, arranging the surface of the beach, dredging draining ditches, removing enemies such as crabs, snails and fishes, draining accumulated water during the hot days in summer, and often observing the growth and development conditions of the young Perinereis aibuhitensis duringthe management at ordinary times, wherein the special bait is prepared by, by mass, 20.0-30.0 parts of peeled soybean meal, 10.0-20.0 parts of domestic fish meal, 5.0-10.0 parts of Siraitia grosvenorii extraction residue, 0.5-1.0 part of 4-D, 0.5-1.0 part of multivitamin and 0.5-1.0 part of composite mineral salt; fourthly, harvesting the adult Perinereis aibuhitensis.