17results about How to "Improve fertilization rate" patented technology

The invention provides an ecology-imitating artificial propagation method for Dongquasichthys nobilis. The ecology-imitating artificial propagation method comprises the following steps: S1, selecting male and female parent fishes and putting the male and female parent fishes into an artificial ecology-imitating breeding pond for breeding; s2, in a non-breeding season, feeding female parent fish feed A for 2-3 times every day, and feeding male parent fish feed B for 2-3 times every day; s3, 2-3 months before breeding, female parent fish fortified feed I is fed 2-3 times every day, and male parent fish fortified feed II is fed 2-3 times every day; s4, after the gonad of the parent fish is mature, artificial spawning induction is conducted, and fertilized eggs are collected and hatched. According to the method, the ecology-imitating breeding environment is manufactured, so that the east astrostroma parent fish adapts to the artificial environment, the irritability is reduced, the east astrostroma parent fish is fed with different feeds in the non-breeding season and the breeding season, the ecological factor and nutritional requirements for gonad development of the east astrostroma can be met, the immunity of the east astrostroma parent fish is improved, gonad maturation is promoted, and the survival rate of the east astrostroma parent fish is increased. The quality of sperms and ova is improved, the fertilization probability is improved, the malformation probability of hatched larvae is reduced, and the breeding efficiency is improved.

The invention provides a breeding method of golden and tiger hybrid spotted fish eggs. The breeding method comprises the following steps: S1, parent screening; s2, parent cultivation; s3, artificial insemination: (1) spawning induction: when the belly of the female parent fish expands, the ovary develops to the IV stage and the male parent fish presses the belly to cause white seminal fluid to flow out, performing oxygenation flowing water stimulation on the female parent fish, and performing irradiation with a TDP radiation lamp for spawning induction; (2) fertilization; s4, artificial incubation: (1) arranging an incubator, injecting seawater, and performing plasma treatment to obtain a treated incubator; and (2) adding the fertilized eggs into the treated incubator, carrying out magnetization treatment, adding chitosan, and hatching to obtain the larva fish. According to the breeding method, the sperm and egg quality can be improved, the fertilization rate and the hatching rate are remarkably increased, and according to verification of the embodiment, the fertilization rate ranges from 97.4% to 99.3%, and the hatching rate ranges from 95.6% to 97.1%. The hatched larvae are not deformed, the survival rate is 96%-99%, the quality of the larvae is high, and a good foundation is established for obtaining high-quality hybrid Jinhuasu.

The invention provides an artificial breeding method of spinibarbus capito, which is suitable for Dongting Lake in Hunan Province and four water basins of Hunan, Zi, active yuan and halite, and comprises the steps of parent fish rearing, artificial spawning induction and artificial incubation, and spawning can be carried out for 1-2 times in one year. In the spinibarbus capito parent fish rearing process, puffed floating fish feed is used as a main material and properly matched with green feed, animal bait is added for feeding 2 months before winter, accumulation of fish body fat can be guaranteed so that the fish can smoothly pass the winter, vitamin E, vitamin C and flowing water stimulation are supplemented in the feed before antenatal, and ovary development and ovum maturation can be effectively promoted; the artificial spawning induction adopts a two-needle injection method, the spawning induction efficiency is high, and the fertilization rate is high; through comparison of different hatching devices, the hatching effect of the hatching frame is best, the hatching rate reaches 80% or above, the hatching barrel is inferior, and the small net cage is worst. By means of the artificial propagation method, large-scale production of spinibarbus capito can be achieved, and the method is of great significance in improving the economic benefits of farmers and popularizing the spinibarbus capito artificial propagation technology.

The present invention provides an antifreeze polypeptide derived from bovine collagen, which can reduce the difference in solute concentration inside and outside the cell during the cooling process, thereby inhibiting the formation of ice crystals inside and outside the sperm cell, and further reducing ice crystal damage to cell membranes and organelles during deep cryopreservation and recovery, thereby improving the survival rate of sperm cells after deep cryopreservation and recovery. The frozen sperm has a higher in vitro fertilization cell development rate and fertility rate, and can reproduce rapidly. In addition, the semen freezing diluent provided by the present application does not contain any exogenous protein, which can effectively reduce exogenous protein interference and immunosuppression during in vitro fertilization, and further improve the fertilization rate and fertility rate of frozen semen.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

The invention relates to a grouper crossbreeding method. The method comprises the four steps of parent breeding, temperature-sensitive domestication, artificial spawning and insemination, incubation and fry breeding, specifically, cold water-based clear water stone spots and hot water-based brown spot stone spots are selected as parents, the clear water stone spots are used as female parents, the brown spot stone spots are used as male parents, and the middle water temperature is adapted through gradient temperature-sensitive domestication; stress-resistant feed containing hilsa herring egg source peptide and haematococcus pluvialis astaxanthin ester and a temperature-sensitive blender are matched, a reproductive care agent is supplemented to strengthen sperm and egg performance, and normative spawning induction, insemination and graded cultivation processes are adopted. According to the method, the problems of poor water temperature adaptation and low hatching rate of grouper hybrid sperms and eggs in different temperature areas are solved, the wide-temperature property of filial generations is improved, the method adapts to the water temperature of 18-28 DEG C, the hatching rate, the fry survival rate, the adult fish survival rate and the average weight of single fish are remarkably improved, and technical support is provided for industrial application.

The invention provides a Bagarius yarrelli artificial propagation method and Bagarius yarrelli sperm storage liquid without emasculation and sperm collection, and belongs to the technical field of aquaculture. The Bagarius yarrelli sperm storage liquid comprises glucose, sodium chloride, potassium chloride, calcium chloride and water. After the Bagarius yarrelli sperm storage liquid is mixed with Bagarius yarrelli sperms, the Bagarius yarrelli sperms can be temporarily stored for one week, and the problem of low fertilization rate caused by asynchronous development of male and female Bagarius yarrelli parents is solved. According to the method, the male Bagarius yarrelli is subjected to induced spawning by adopting a two-needle method, firstly, luteinizing hormone releasing hormone A < 2 > + domperidone maleate is injected, after 9-11 h, the luteinizing hormone releasing hormone A < 2 > + domperidone maleate + chorionic gonadotropin is injected, and the injection dosage is strictly controlled. By means of the method, artificial breeding, large-scale production and enhancement and release of the Bagarius yarrelli can be achieved under the condition that male fish testis are not cut, and the method has important significance on protection of the Bagarius yarrelli.

The invention belongs to a freshwater fish breeding technology, and particularly relates to a phoxinus lagowskii large-scale breeding method which sequentially comprises the following steps of parent selection, parent breeding, artificial spawning induction, artificial insemination and juvenile fish hatching. Injecting a spawning induction agent, which is composed of 500-1000 IU of chorionic gonadotropin (HCG) per kilogram of fish body weight, 5-10 micrograms of luteinizing hormone releasing hormone analogue (LRH-A2) per kilogram of fish body weight and 1-2 mg / kg of domperidone (DOM) per kilogram of fish body weight, into the bred parent from pelvic fins; the injection dosage of the parent female fish is two times that of the parent male fish. The method solves the problems that phoxinus lagowskii parents are asynchronous in gonad development, do not lay eggs and are not concentrated in egg laying, the induced spawning rate is increased to 98% or above, and meanwhile the production time is shortened. By means of manufacturing and using the artificial fish nest, the natural hatching state of phoxinus lagowskii roes is simulated, damage to the roes in the debonding process is reduced, and the fertilization rate and hatching rate of the roes are increased to 96.8% and 92.1% respectively.

The invention provides a method for artificial insemination and fertilized egg incubation of wild schizothorax parvus, which comprises the following steps: on the basis of ascertaining the biological characteristics of the wild schizothorax parvus through tracking and monitoring for many years, capturing sexually mature male and female parent fishes, carrying out artificial insemination after sperm and egg treatment, obtaining fertilized eggs, and carrying out micro-running water incubation, so that the fertilization rate reaches 85%, and the incubation rate reaches 70%. The artificial insemination of the rare and endangered indigenous fish Schizothorax parvum in the Luagu Lake and the trial culture of the first filial generation of fish fries are successfully carried out for the first time in China, the blank in the field is filled, the artificial domestication, breeding and culture of the Schizothorax parvum are completely feasible and are preliminarily successful, and the specific fish population of the Luagu Lake is recovered, developed and cultured, so that the economic benefits of the Luagu Lake and the Schizothorax parvum are improved. High-quality offspring seeds are provided, and the method has important significance on lake enhancement and release and improvement of fishery economic benefits.

The invention relates to a crossbreeding method for babylonia lutosa and babylonia taiwan. The cross breeding method comprises the following steps: putting Babylonia taiwanensis and Babylonia lutosa into independent units in a dark environment for breeding, hybridizing, collecting oocysts, disinfecting the oocysts, and hatching to obtain Babylonia lutosa planktonic larvae; a fertilizer water agent and a nutritional agent are applied to culture water of babylonia planktonic larvae, unicellular algae are mixed for culture, and the babylonia planktonic larvae are metamorphosed and attached to form juvenile snails. Compared with the existing Babylonia taiwanensis and Babylonia lutosa, the Babylonia lutosa hybrid fry bred by the crossbreeding method not only has more excellent metamorphosis rate, but also has significantly improved fertilization rate, egg laying amount, hatching rate and larva survival rate.

A method for promoting ovarian development of pelteobagrus vachelli belongs to the technical field of fish culture, and is characterized in that in the pelteobagrus vachelli culture process, a functional additive is fed at the ovarian development preparation stage and the ovarian development strengthening stage, and the functional additive is prepared by mixing epigallocatechin gallate and compound unsaturated fatty acid, adding the compound unsaturated fatty acid into the mixture, and stirring to obtain the functional additive for promoting the ovarian development of the pelteobagrus vachelli. Beta-cyclodextrin is firstly adopted for inner layer embedding, then chitosan is adopted for outer layer coating, the microcapsule is prepared, and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid according to the volume ratio of (0.8-1): (1-1.2). EGCG and compound unsaturated fatty acid are compounded and embedded to prepare microcapsules, the microcapsules are added into feed for feeding, ovarian development of pelteobagrus vachelli is effectively promoted, the number of oocytes of pelteobagrus vachelli is increased, meanwhile, the cell proportion of III-stage oocytes is increased, the reproductive capacity of pelteobagrus vachelli is effectively improved, the survival rate of pelteobagrus vachelli is increased, and the survival rate of pelteobagrus vachelli is increased. The egg laying amount, the fertilization rate and the hatching rate are improved.

The application provides alligator feed, an alligator feeding method and application, and relates to the technical field of alligator breeding. The alligator feed with high meat yield rate comprises fish, fish meal, vitamins, calcium powder, medicinal materials and probiotics; the fish meal comprises fish, shrimps and land animal meat; and the medicinal materials comprise at least one of malt, houttuynia cordata and hawthorn. Through the alligator feeding method, the alligator feed is fed, the meat yield rate, the egg production rate of alligators, and the fertilization rate and the hatching rate of alligator eggs are improved, the alligator feeding system has high economic benefits, and the preparation method of the alligator feed is simple and efficient.

The invention discloses an artificial propagation method for culter oxyculter. In the breeding peak period of each year, namely from late May to mid-late June, parent fishes with good sexual maturity are selected from artificially domesticated culter oxyculta for artificial breeding, and the female-male ratio is 1: 2. Injecting artificial spawning induction medicine into the parent fish, and after effect time is reached, respectively taking seminal fluid and ova of the male fish and the female fish for artificial insemination. Fertilized eggs are incubated in the incubation pond for about 5 days and then transferred into the pond for cultivation after swimming flatly, and rotifers cultivated in the pond are mainly used as initial feed. And starting to gradually feed compound feed for domestication when the rotifers grow to about 3cm after being fed for one month. The artificial propagation method disclosed by the invention has the characteristics of high induced spawning rate, high fertilization rate, high hatching rate, high survival rate and the like, and also has the characteristic of low parent death rate. The method is suitable for large-scale culter oxyculter fry breeding as required, and has the advantages of high efficiency, simplicity, convenience and facilitation of popularization. The method provided by the invention provides solid theoretical and technical support for resource protection and aquaculture variety cultivation of culter oxyculter.

The invention discloses an artificial propagation-hybridization combined breeding method for meat goats, and relates to the technical field of animal breeding, and the method comprises the following specific steps: selecting a goat variety with good meat performance as a female parent, and selecting a goat variety with large physique as a male parent; the method comprises the following steps: performing in-vitro culture on female parent oocytes to obtain mature oocytes containing first polar bodies, screening high-activity sperms by adopting a temperature gradient screening device, performing in-vitro fertilization on the mature oocytes and the high-activity sperms, culturing the mature oocytes and the high-activity sperms to a hybrid blastocyst form, transplanting the mature oocytes and the high-activity sperms into a female body, and developing the mature oocytes and the high-activity sperms until the mature oocytes and the high-activity sperms are naturally delivered to obtain a hybrid F1 generation; backcrossing the F1 generation with the female parent, backcrossing the backcrossed offspring with the female parent for multiple times until a breeding target is met, and carrying out cross-crossing fixation to obtain a new variety / line with excellent characters; the sperm screening efficiency is improved by utilizing the screening device, and the quality of the screened sperms is ensured, so that the fertilization efficiency and the embryo survival rate are remarkably improved.

The invention belongs to the technical field of livestock artificial insemination, and relates to a livestock intrauterine continuous insemination device which comprises a device body, an injection channel is arranged in the device body in a penetrating mode, an injection head is arranged at one end of the injection channel, a filling hole is formed in one side of the device body and communicated with the injection channel, and a sealing cover is detachably arranged in the filling hole. The device further comprises a protection mechanism and a reset mechanism. The first protective shell is matched with the second protective shell, the first protective shell and the second protective shell are made to rotate inwards through the reset mechanism, and the first protective shell and the second protective shell are oppositely buckled to form the cavity for containing the injection head, so that the injection head is protected, and the injection head is protected when the device is idle and carried; by arranging the filling holes, semen samples do not need to be repeatedly supplemented when different female livestock individuals are injected, the flexibility of continuous insemination is improved, meanwhile, the infection risk during female livestock insemination is further reduced, and the operation difficulty is also reduced.

The invention provides a method for promoting ovarian development and healthy breeding of Chinese softshell turtles in a hibernation period, and relates to the technical field of Chinese softshell turtle breeding, the method comprises the following steps: S1, fishing female sexually mature Chinese softshell turtles after the Chinese softshell turtles enter the hibernation period, and cleaning the surfaces of the Chinese softshell turtles; s2, the healthy Chinese softshell turtles are subjected to single wrapping feeding in a low-temperature water environment, and then the Chinese softshell turtles are induced to enter a hibernation state under the low-temperature condition; s3, after the pond of the Chinese softshell turtles is cleaned, water in the pond is drained, and bottom mud is disinfected and improved in the hibernation season; s4, performing creatine intraperitoneal injection on the Chinese softshell turtles; s5, performing water storage and fertilizer and water management on the disinfected pond; s6, putting the Chinese softshell turtles subjected to creatine treatment in proportion when the air temperature is appropriate; compared with the prior art, the method has the following advantages that 1, ovarian development of the Chinese softshell turtles in the hibernation period is stimulated through a non-hormone method, and food safety is guaranteed; hibernation is carried out without the bottom mud environment of the culture pond, and the hibernation environment of the Chinese softshell turtles is improved; 3, single feeding and intensive management are realized, so that the survival rate of hibernation is increased; 4, when the sleeping environment is met in the second year and the temperature change is mild, pond entering treatment is carried out, the female-male proportion is reasonably matched, and disease outbreak caused by dead individuals is avoided; 5, while the parents are independently bred in an intensive manner, the bottom mud of the outer pond can be disinfected, exposed to the sun and the like, and an excellent environment is created for cultivation in the second year; and 6, through creatine injection, early development of the ovary of the Chinese softshell turtle can be effectively stimulated under the condition that the natural process of hibernation is not influenced, so that the sexual maturity period can be shortened, and the breeding efficiency can be improved.

The present invention provides a method and application for optimizing the fertility control of female fish. The method uses gene editing technology to knock out the key coding gene for the synthesis of gonadal progesterone in fish, block the synthesis of fish progesterone, and hinder the maturation and ovulation of the oocytes of the obtained homozygous female fish, so that it is unable to mate and lay eggs with wild-type male fish, thereby obtaining infertile female fish. After the obtained infertile female fish is treated with gonadotropin and corresponding preparations, it can restore the ability to mate and lay eggs naturally with wild-type male fish, and achieve a higher fertilization rate and membrane emergence rate, and the surviving offspring individuals have no obvious difference in morphology from wild-type fish of the same period. In the above manner, the method provided by the present invention can be widely used in various types of aquaculture fish to achieve effective control of the fertility of female fish in aquaculture fish, and protect ecological safety, and has broad application prospects in the field of genetic breeding and ecological safety of aquaculture fish.