1results about How to "Improve fertilization rate" patented technology

The invention relates to a grouper crossbreeding method. The method comprises the four steps of parent breeding, temperature-sensitive domestication, artificial spawning and insemination, incubation and fry breeding, specifically, cold water-based clear water stone spots and hot water-based brown spot stone spots are selected as parents, the clear water stone spots are used as female parents, the brown spot stone spots are used as male parents, and the middle water temperature is adapted through gradient temperature-sensitive domestication; stress-resistant feed containing hilsa herring egg source peptide and haematococcus pluvialis astaxanthin ester and a temperature-sensitive blender are matched, a reproductive care agent is supplemented to strengthen sperm and egg performance, and normative spawning induction, insemination and graded cultivation processes are adopted. According to the method, the problems of poor water temperature adaptation and low hatching rate of grouper hybrid sperms and eggs in different temperature areas are solved, the wide-temperature property of filial generations is improved, the method adapts to the water temperature of 18-28 DEG C, the hatching rate, the fry survival rate, the adult fish survival rate and the average weight of single fish are remarkably improved, and technical support is provided for industrial application.