637results about How to "Improve fertilization rate" patented technology

The invention provides a technique for preparing hermaphrodite scallop interspecific hybrid fingerlings, which relates to a marine shellfish breeding technique. Purple scallops and bay scallops are fattened and induced to be mature respectively so as to reach sexual maturity at the same time, and then are synchronously induced to lay eggs and discharge sperms respectively, so that sperms and eggs of two types of scallops are obtained respectively to perform interspecific cross insemination. The purple scallops and the bay scallops are hermaphrodite, both belong to an Argopecten category, have the same number of chromosomes, and have complementary superiority and traits. Hybrid groups are high in both fertilization rate and hatching rate and normal in larvae development. Two types of scallop parents adopted have complementary superiority and traits, thereby having significant heterosis in size, growth, adaptability and other aspects. As two types of scallops are both hermaphrodite, parent scallops are easy to purifym and the offspring with greater heterosis can be obtained by hybridizing the purified parent scallops.

The invention discloses a loach fish fry artificial propagation method. The loach fish fry artificial propagation method comprises the following steps: parent fish rearing and selecting; artificial induction; fertilization process; and fertilized egg hatching process. The loach fish fry artificial propagation method can excellently improve induction rate, fertilization rate and hatching rate, and especially improves post-natal survival rate of the parent fish. The loach fish fry artificial propagation method can achieve loach fish fry industrialized production, provides offspring seeds for large-scale farming, and acquires excellent economic efficiency, by using the loach fish fry artificial propagation method the post-natal survival rate of the parent fish is 90.87 percents, increasing by 40.87 percents; the induction rate is 91 percents, increasing by 11 percents; the fertilization rate is 96.67 percents, increasing by 1.67 percents; and the hatching rate is 91.07 percents, increasing by 11.07 percents.

The invention discloses a vespa mandarinia breeding technology and belongs to the breeding technology for special insects. The vespa mandarinia breeding technology includes building sheds, preparing boxes, preparing materials, taking larvae, coupling, overwintering, swarming and training the adaptability to the field environment. Male and female bee larvae taken from the field couple, overwinter and nest in different sizes of bee sheds and bee boxes in a manual feeding mode, and the bee larvae are moved to the field after being trained. The vespa mandarinia breeding technology saves labor force and bee force; the vespa mandarinia breeding technology is low in cost, high in output and especially suitable for artificially breeding scoliid populations.

The invention discloses a tortoise breeding method, which includes the following procedures: 1 choosing of an adult tortoise pool; 2 construction of a spawning ground, feed tables and terrances; 3 the adult tortoise pool cleaning and sterilization; 4 reserved adult tortoises screening; 5 choosing of an adult tortoise; 6 sterilization of the adult tortoise; 7 free breeding of the adult tortoise; 8 spawning of the adult tortoise; and 9 incubation of tortoise eggs. The spawning ground is built on the east side of the adult tortoise pool, a simple rain shelter is constructed above the adult tortoise pool, the feed tables are built on two sides of the middle portion of the adult tortoise pool and 10cm to 15cm above the water surface, and two terrances made of moso bamboos are constructed in the adult tortoise pool. In spawning of the adult tortoise, firstly the spawning ground needs to be cleaned and sterilized, and the tortoise eggs need to be gathered after the spawning of the adult tortoise. The tortoise breeding method remarkably improves egg laying amount, fertilization rate, hatchability rate and proportion of female tortoise seeds of adult reeve's turtles.

The invention relates to an artificial reproduction method for schizothorax wangchiachii. The method mainly comprises the following seven steps: bait preparation, parent fish sourcing, parent fish domestication, parent fish selection, artificial insemination, fish egg incubation and fish fry feeding. The artificial reproduction method for schizothorax wangchiachii has the beneficial effects that oxytocin is not used, the schizothorax wangchiachii can lay eggs and produce sperms, and relatively high fertility rate and hatchability can be obtained; the problem that female and male wild fish do not develop or develop asynchronously in a pond culture environment is solved fundamentally; the damage on the parent fish of the schizothorax wangchiachii is reduced, and the cultivation cost is also reduced; the method is reasonable and efficient, the hatchability is improved, and the waste of fish egg resources is reduced; and the aberration rate of the fish fry is reduced while the survival rate of the fish fry is improved.

The invention discloses a human ovocyte cryoprotectant. The human ovocyte cryoprotectant contains an ovum refrigerating fluid and an ovum thawing fluid, wherein the ovum refrigerating fluid contains a balance fluid and a refrigerating fluid; the ovum thawing fluid contains a resuscitation fluid, a diluent-1, a diluent-2 and a cleaning solution. The human ovocyte cryoprotectant is developed by combined application of the propanediol and glycol permeable cryoprotectants, and taking the trehalose (trehalose, alpha-D-glucopyranosyl alpha-D-glucopyranoside, C12H22O11) as a non-permeating agent. The non-permeating cryoprotectant suitable for freezing the human ovocyte is developed so that the formation of the ice crystal in the cell can be inhibited, the function of the frozen ovum can be protected and the developmental potentiality of the frozen ovocyte can be greatly improved. The clinical result proves that the fertility rate, the cleavage rate and the high quality blastocyst rate of the frozen ovocyte can be greatly improved.

The invention discloses a chicken feed probiotic agent containing enterococcus faecalis and a preparation method of the chicken feed probiotic agent. The chicken feed probiotic agent is prepared by virtue of the steps of evenly mixing enterococcus faecalis HEW-A131 active bacterial sludge, bacillus subtilis active bacterial sludge, saccharomyces cerevisiae active bacterial sludge and a stabilization protective agent together in the mass ratio of (0.1-0.8): (1-2.5): (0.1-0.5): 1, and then pelletizing, drying, coating and the like; the probiotic agent is capable of improving the utilization efficiency of an animal feed and saving the cost, and also capable of greatly improving the steady state of the internal environment of the digestive tract in an animal body, promoting the absorption and utilization of nutrition and promoting the growth of the animal; as a result, the productivity, the immune performance and the reproductive performance of the animal are obviously improved, the production cost is reduced and the economic benefit is increased.

The invention introduces a method for improving the reproduction rate of giant salamanders, which includes the following steps: (1) building up an artificial natural ecological function culture system, namely, at least one artificial natural ecological function culture pond group, wherein each pond group comprises 2-3 rearing ponds; (2) feeding qualitatively and quantitatively at fixed time, wherein live loaches or crucians are taken as the main feed, fresh shrimps and beef liver or pork liver are added as auxiliaries, furthermore as for male salamanders, testes of cocks or ducks are added, and as for parent salamanders, vitamin C or vitamin E are added before and after the parturition; and (3) regulating and controlling the water temperature, wherein at the temperate range of 17-19 DEG C, the female salamanders and the male salamanders can be enabled to grow up at the same time by rising or lowering the water temperature by 1-2 DEG C. The method can greatly improve the artificial reproduction rate of giant salamanders, with the induced spawning rate of the female salamanders reaching 75% and the male salamanders exceeding 80%. Meanwhile, the method can also improve the fertility rate and the hatching rate by 8-12%.

The invention provides a manual schizothorax griseus pellegrin breeding method and belongs to the technical field of wild fish domestication and breeding, freshwater aquaculture and fish genetics-breeding. The manual schizothorax griseus pellegrin breeding method includes the following steps that 1, parent fish are caught and domesticated; 2, cultivation and spawning induction are conducted on the parent fish; 3, artificial insemination and egg hatching are conducted; 4, fry rearing is conducted. The manual schizothorax griseus pellegrin breeding method has the advantages that no report on the manual schizothorax griseus pellegrin breeding method exists in documents disclosed both home and abroad; the breeding method is scientific and reasonable, the rate of fertilization and the hatching rate of fries are higher and reach 98.6% and 91.2% respectively; the problem that no fry and fingerling exist for wild schizothorax griseus pellegrin resources is solved through manual recovery and supplement; the problem that no fry is supplied in the manual schizothorax griseus pellegrin breeding process is solved.

The invention discloses a high strength selecting and cultivating method of excellent stichopus japonicus seeds. The high strength selecting and cultivating method comprises the steps of carrying out cultivation, spawning or spermiation promotion, and hybridization on parent stichopus japonicuses from at least four different geographical groups, and carrying out selective breeding at different degrees in different periods according to a quantitative genetics method. By combining two traditional and effective methods of hybridization breeding and selective breeding, the invention preserves excellent properties of the stichopus japonicus and also avoids inbreeding; because the cultivated stichopus japonicuses have excellent properties of different groups, the cultivated stichopus japonicuses have the advantages of fast growth speed, strong stress resistance and the like; and the invention not only realizes seedling pre-culturing of the stichopus japonicus to meet market demand in advance, but also prolongs an effective growth period of the stichopus japonicus within a year, shortens cultivation time, and improves economic benefit; therefore, the inveniton is suitable for large-scaled factory production.

The present invention provides an animal and poultry compound premix, which is suitable for being used for producing perfect compound feed for chickens and pigs in different physiology phases. The components of the animal and poultry compound premix of the present invention comprises VitA, VitD3, VitE, VitK3, VitB1, VitB2, VitB6, VitB12, pantothenic acid, nicotinic acid, folic acid, biotin, choline chloride, iron salt, zinc salt, cupric salt, manganese salt, iodine salt, selenote and carriers, and also comprises cobalt salt and lysine. The present invention provided the animal and poultry compound premix comprising various vitamin and microelement with the advantages of high nutritive material content, high feed transforming rate, healthy and high survival rate of the animal and the poultry after eating the premix, the dejection viscosity reducing, which is beneficial for cleaning and reducing the environment pollution; the egg laying rate of the breeding hens is high and the fertility rate and the hatching rate of the breeding hens is improved after being eaten by the breeding hens and the weights of hens are even.

The invention discloses a medicinal composition, which comprises Chinese medicinal materials 30-99.9% of the total weight of the composition, and pharmaceutically acceptable carries in balancing amount. The Chinese medicinal materials include the following Chinese traditional medicines by weight: (a) 15-90 parts of Chinese medicinal materials for providing sperms motion energy, (b) 15-60 parts of Chinese medicinal materials for strengthening adenosine triphosphate, (c) 70-330 parts of Chinese medicinal materials for providing antioxidation effect, (d) 10-50 parts of Chinese medicinal materials for promoting sperms generation, (e) 10-40 parts of Chinese medicinal materials for regulating internal secretion, and (f) 20-80 parts of Chinese medicinal materials for invigorating qi and strengthening spleen. The invention also discloses a preparation method and an application thereof.

The invention relates to a novel poultry micro-ecological preparation and a preparation method of the novel poultry micro-ecological preparation. The preparation comprises following components in part by weight: 0.8-3.5 parts of vitamin A, 0.2-0.8 part of vitamin D, 2-8 parts of vitamin E, 0.2-1.2 parts of vitamin K, 0.1-0.9 part of vitamin B1, 0.2-0.8 part of vitamin B2, 0.1-0.7 part of vitamin B6, 0.08-0.5 part of vitamin B12, 2-5 parts of nicotinamide, 0.8-3.5 parts of calcium pantothenate, 0.02-0.4 part of folic acid, 0.2-1.2 parts of biotin, 0.02-2 parts of vitamin C, 1.3-32 parts of enterococcus faecium, 27-155 parts of bacillus subtilis, 75-210 parts of oligosaccharide, 150-400 parts of pine needle meal and 400-600 parts of bentonite. The novel poultry micro-ecological preparation can improve daily gain, feed intake, feed conversation efficiency of young poultry, reduces death and culling rate of the young poultry, and improves reproductive performance of breeding poultry, laying rate, fertilization rate of hatching eggs, hatching rate, and healthy rate of the young poultry. According to the invention, as double-layer shallow coating is performed to enterococcus faecium, the stress resistance of enterococcus faecium is improved.

The invention belongs to the technical field of feed, particularly relates to egg production stage mixed feed for increasing the rate of healthy chicks of egg breeding chickens and relates to a preparation method of the feed. The mixed feed is mainly prepared from, by weight, 50-65 parts of grains, 15-25 parts of soymeal, 1-5 parts of corn protein flour, 1-6 parts of rice bran meal, 2-6 parts of alfalfa meal, 1-3 parts of vegetable oil, 0.35-0.7 parts of amino acid, 0.05-1 part of compound enzyme, 0.05-2 parts of probiotics, 0.01-0.03 parts of 25-hydroxycholecalciferol, 0.01-0.3 part of beta-carotene, 0.03-0.08 part of compound organic trace elements and 1 part of compound premix. By means of sufficient and efficient energy, amino acid, mineral substance nutrients and probiotics, the requirement for the highest egg production potential of the egg breeding chickens can be guaranteed, the egg production rate is high, egg weight is appropriate, and the rate of the healthy chicks of the egg breeding chickens is high.

The invention discloses an intensified artificial breeding method for Australian crayfishes. The method comprises the following steps of: putting pubescent parent crayfishes which pull through a wintering period into a spawning pond according to a proportion of 1-3: 1 of female and male, wherein circulating water is adopted in the spawning pond to automatically control and increase oxygen; sterilizing fertilized ovigerous female crayfishes, putting into small hatching lattices in a hatching pond, wherein one crayfish is hatched in each small hatching lattice, and carrying out water-flow hatching; and enabling hatched young crayfish seeds to leave away from mother bodies after 30-50 days of incubation period, enabling the young crayfish seeds to escape into water through a filter screen of the hatching pond, after all the crayfish seeds at the abdomen of the ovigerous female crayfishes leave away from the mother bodies, immediately separating the parent crayfishes, and remaining the young crayfish seeds to be continuously cultivated for 5-10 days, so that the cultivation can be carried out. According to the method, the artificial intensified breeding incubation can be carried out according to the required offspring seed quantity and the required offspring seed time at any time, the water temperature, the dissolved oxygen, the water quality and the like can be controlled, the method can not be influenced by environment, and the method is high in fertilizing rate, spawning rate and hatching rate.

The invention relates to a cultivation method of economic seaweed, in particular to a full-artificial seed cultivation method for sargassum horneri. Concretely, the method comprises the following steps: selecting male and female sargassum horneri parent strains with good pyrenium growth to be cultivated for 10 to 20 days under the conditions that the illumination intensity is between 2500 and 10000 luxes and the temperature is between 10 and 16 DEG C for realizing the synchronous sexual development and sexual maturation of the sargassum horneri so that ova and sperms are discharged synchronously for impregnation; completing the collection and the seedling distribution of germ cells in 10 to 24 hours after the impregnation; carrying out static cultivation for 2 to 4 days after the seedling distribution; and maintaining the water flowing under the condition of the illumination intensity between 400 and 8000 luxes for completing the cultivation. The seed cultivation process of the invention is carried out in an artificial cultivation system, so the impregnation rate can be further and greatly improved, the spatfall efficiency and the full-artificial seed cultivation efficiency can be improved.

The invention belongs to the technical field of insect feed additives, and particularly discloses an insect additive for poultry and livestock feeds and application of the insect additive. The additive comprises the following components in part by weight: 20 to 50 parts of insect albumen powder, and 1 part of chitin and antimicrobial peptide. The chitin and the antimicrobial peptide which are extracted from insects are used for compounding the additive for the feeds, and the quality of the feed is improved, so that the quality of raised animals is effectively improved, and safe and healthy meat and fish or egg products are produced.