192 results about "Artificial reproduction" patented technology

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

An artificial reproduction method for schizothorax includes choosing parents fish, culturing, fertilization, incubation and culturing fry.

The invention discloses a grouper large scale total-artificial breeding method. The method comprises the steps of parent cultivation, artificial reproduction, offspring seed production, leaving pond of offspring seeds, and temporary rearing in a net cage. The grouper large scale total-artificial breeding method is suitable for seedling cultivation of orange-spotted groupers, or saddletail groupers, or giant groupers, or Hong Kong grouper, or East Star Groupers. The grouper large scale total-artificial breeding method solves the problem that the death rate of groupers in a current seedling cultivation technology is high, and broad prospect of scale development of grouper cultivation is achieved.

The invention relates to a simulated ecological breeding method for Chinese soft-shelled turtles, which belongs to the technical field of soft-shelled turtle breeding, the steps are as follows: (1) environmental condition; (2) parent turtle breeding; (3) artificial reproduction and incubation; (4) breeding of turtles; the breeding method meets the growing physiological needs of the turtles to the largest extent in a loose and stable environment, the turtles bred outside cages fall ill less, drug abuse is avoided effectively, the turtles have higher value of commodity, the taste is like that of a wild turtle, and the simulated ecological breeding method has a shorter breeding period than ecological breeding.

The invention relates to an artificial reproduction method for schizothorax wangchiachii. The method mainly comprises the following seven steps: bait preparation, parent fish sourcing, parent fish domestication, parent fish selection, artificial insemination, fish egg incubation and fish fry feeding. The artificial reproduction method for schizothorax wangchiachii has the beneficial effects that oxytocin is not used, the schizothorax wangchiachii can lay eggs and produce sperms, and relatively high fertility rate and hatchability can be obtained; the problem that female and male wild fish do not develop or develop asynchronously in a pond culture environment is solved fundamentally; the damage on the parent fish of the schizothorax wangchiachii is reduced, and the cultivation cost is also reduced; the method is reasonable and efficient, the hatchability is improved, and the waste of fish egg resources is reduced; and the aberration rate of the fish fry is reduced while the survival rate of the fish fry is improved.

The invention discloses an artificial reproduction technological process and a method of application of aphidoletes aphidimyza. An aphidoletes aphidimyza mass reproduction technology and an application technology are characterized in that pea aphids, pea repaired tail aphids and aphis craccivora koch are reproduced massively by adopting broad beans, and the aphidoletes aphidimyza is reproduced by utilizing the pea aphids, the pea repaired tail aphids and the aphis craccivora koch. The aphidoletes aphidimyza mass reproduction technology and the application technology comprise the following processes: net seedlings are planted, aphids are raised, the aphidoletes aphidimyza is raised, old larvae are collected, and the larvae are saved in the pupal period. Adults are applied to field application and expanding reproduction after eclosion. An efficient and superior method technological process for reproducing aphidoletes aphidimyza is developed so as to solve the to-be-solved problems, and the method that the aphidoletes aphidimyza is used to prevent and control aphids can play a proper role in safe production of vegetables.

The invention discloses an artificial breeding method for Epinephelus tukula. The method comprises the following steps: (1) selecting parents; (2) culturing the parents in a pond; (3) culturing parent fishes in an anestrous season; (4) performing strengthening cultivation before reproduction; (5) performing artificial reproduction; (6) collecting and treating oosperm; (7) hatching the oosperm. Through parent culture of the Epinephelus tukula, parent fishes are made to lay eggs, and the oosperms are used to hatch fries, and the fries are cultured into fingerlings for breeding. The method provides a reasonable solution which is high in hatching rate for developing cultivation of Epinephelus tukula in a large-scale manner.

Disclosed is a method for breeding mandarin fish by utilizing microorganic feed. The method includes that: a pond is built; the pond requires a sandy bottom, about 15cm of sludge in depth, deeper than 1.5m of water, convenient irrigation and drainage, good water quality and no sewage inflow; environment requires: parent fish are selected at the most suitable growing water temperature which is 25-29 DEG C in artificial breeding application; in winter and spring, the parent fish healthy in physique and woundless in body surfaces are captured from a breeding pond and put into the pond in a greenhouse for artificial reproduction; the parent fish are put into the pond in the greenhouse for cultivation, and during daily breeding management, in the breeding period, the feed is put for 4 times every day, the amount of the feed is calculated according to 25-50% of the weight of the fish, and the feeding degree is determined according to the fact that no fish competes for food and is flexibly mastered according to weather conditions. Due to the addition of the compound microorganic feed, quick growth of the mandarin fish can be facilitated, EPA (eicosapentaenoic acid) content is increased, illness of the mandarin fish is decreased, the mandarin fish died of disease are decreased, beneficial microorganisms in water are increased, and quality and mass are increased finally.

The invention relates to an artificial propagation method of fry of paracobitis variegatus. The artificial propagation method comprises the steps of parent fish rearing, parent fish selection and mating, artificial spawning, fertilization, oosperm hatching, larva fish temporary rearing and fry rearing. The artificial reproduction technology of the paracobitis variegates is successfully summarized in the artificial propagation method, and the artificial propagation method fills the blank of the technology. By adopting and popularizing the artificial propagation method having been successfully studied out, the fry of the paracobitis variegatus can be obtained in batches in a centralized mode, the spawning rate, the fertilization rate and the hatching rate are high, and large-scale production of the paracobitis variegatus can be easily achieved. Thus, the artificial propagation method has great significance on protection and large-scale rearing of the paracobitis variegatus wild resources.

Disclosed is an indoor artificial reproduction method of monoparasitism apanteles ruficrus haliday. The method includes the steps of host breeding and bee breeding; host breeding refers to taking prodenia litura larvae of two to three years old as reproduction hosts, transferring part of the larvae into an environment of 6 DEG C for breeding to be old larvae for pupation, and forming generation overlapping of host larvae; bee breeding includes bee inoculating, cocoon management, adult bee management the like, bee inoculating refers to placing leaves with traces formed by gnawing of the host larvae and five healthy prodenia litura larvae of two to three years old in a flat-bottom test tube of 2x10cm for inoculating for 2 hours, and cocoon and adult bee management needs to be performed under relatively proper conditions and needs to be matched with corresponding management technology. The indoor artificial reproduction method is easy to get in materials, high in reproduction rate and high in stability.

An artificial reproduction method for drynaria includes asexual production and spore reproduction. Said asexual reproduction includes such steps as taking its rhizome segment with 3-5 bud points and 3-5 sporophyllary leaves, fixing it to tree or mountain rock, covering it by bryophyte, spraying water and management. Said spore reproduction includes such steps as sterilizing the burnt earth, immersing in cold boiled water, loading the sporanges in bag, drying in air, preparing the suspension of sporanges, spraying it onto said burnt earth, optical culture to obtain gametophytes, transplanting, culturing to obtain sporophytes, and transplanting them on tree or mountain rock.

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

The invention relates to a semi-artificial bionic breeding method of a finless eel. The method is characterized by adopting the following five processes: obtaining and selecting a parent finless eel; ripening the parent finless eel and carrying out hasten parturition on the parent finless eel; preparing a bionic breeding pool (mating and spawning pool) condition; managing mating, spawning and incubating, catching and cultivating eel seeding, and transferring the captured eel seeding into a special pool to cultivate. According to the semi-artificial simulated ecological breeding method, the breeding yield and the efficiency of the finless eel can be effectively improved; the brood amount, the spawning rate, the fertility rate and the seeding survival rate of the parent can be effectively improved by simulating natural simulated ecological breeding, and organically combining artificial propagation with the natural breeding habit of the finless eel through a proven technique of artificial ripening and hasten parturition commonly used on aquatic products, and the survival rate of the parent is also obviously increased in comparison with a whole artificial reproduction technology. Thus, the breeding yield and the efficiency of the finless eel are improved, and the enough seed matching is provided for large-scale cultivation of the finless eel.

The invention discloses a method for artificial breeding of reef-dwelling young holothuria leucospilota in a small water body. According to the method, through bait and water quality management in keystages such as the pelagic stage, the metamorphosis settlement stage and the young holothuria leucospilota stage, artificial breeding of young holothuria leucospilota is achieved, artificial reproduction and releasing of holothuria leucospilota are made possible, the restoration of the marine benthic ecological environment of the South China Sea is facilitated, and basic conditions are provided for further development and utilization of biological resources of holothuria leucospilota.

An artificial reproduction method for lateen mussel or butterfly mussel includes such steps as choosing more than 3 years old parents, temporarily culturing in adult mussel pool, injecting the chorionic gonadotropin in female parent, culturing the female parents in individual pool, taking the seminal fluid from male parent, diluting by sodium chloride solution, injecting it in female parent twice, culturing in temporary pool until glochidium is mature, parasitizing and culturing young mussel.

An artificial reproduction technique for craspedacusta includes such steps as capturing the parents with high activity, complete body and visual sexual gland, choosing and pairing, culturing in the water with pH=6.4-8.6 and dissolved oxygen (3.0-21.7 mg / L) under illuminance of 800-1200 Lx, feeding small crustacena plankton and seaweed, culturing hydroids, culturing larvae, and culturing adults.

An artificial reproduction method for half-smooth tongue-sole features that the hydrological environment factors for culturing parent fish are controlled for naturalizing them, the growth environment conditions of parent fish are controlled for surviving the winter, and the illuminance and water temp are controlled for ensuring the normal development of the gonad of parent fish.

The invention relates to the field of artificial reproduction of rare trees and in particular relates to an artificial seedling reproducing technique for huanghua pear trees. The artificial seedling reproducing technique for the huanghua pear trees is characterized by specifically comprising seedling growing, soil preparation, fertilizer application and backfilling, planting and nursing management. According to the artificial seedling reproducing technique for the huanghua pear trees, high survival rate of huanghua pear cuttings is realized by use of the scientific and reasonable methods such as seedling growing, soil preparation, fertilizer application and backfilling, planting and nursing management, artificial seedling reproduction of the huanghua pear trees is realized by virtue of seedling management, and reasonable prevention and treatment and nursing management, and finally, extensive management is realized on the huanghua pear trees to allow the huanghua pear trees to grow naturally.

The invention relates to a giant salamander breeding method considering imitative ecological reproduction and artificial reproduction; existing artificial reproduction technologies mostly employ steroid injection spawning, thus causing large damages on giant salamander; the novel method builds a giant salamander natural habitat environment on one hand, and adds infrared sensors in artificial caves of the giant salamander; the infrared sensors are connected with a monitoring computer through a data line; when the monitoring computer discoveries that the infrared rays sent by infrared ray emitters of certain artificial cave are blocked for more than 2 minutes, the giant salamander in the artificial cave is determined to have crawled to a showering pipe for showering once; if the giant salamander of certain artificial cave has 5 or above times of showering within 12 hours, the giant salamander is determined to be natural reproduced giant salamander, steroid injection spawning is not needed, and imitative ecological reproduction is carried out; steroid injection spawning is carried out for the resting giant salamander. The giant salamander breeding method considering imitative ecological reproduction and artificial reproduction is high in breeding rate, good in population biology, and suitable for culturing and breeding giant salamander.

The invention discloses a method for nursing wild coreius guichenoti. Nursing injection liquid is injected into fish according to the fish size, then water is packaged in a plastic bag and oxygenated, and the fish is transported to a culture place. Before the fish is placed into a pond, a 3% NaCl solution is used for soaking the fish for 10 minutes, erythromycin ointment is used for coating wounded portions, after the fish is placed into the pond, nursing injection liquid is injected to the fish one by one every other day twice, and culture management is conducted. The nursing injection liquid is mainly composed of antibiotics, anti-stress medicine and energy mixture. By means of corresponding medicine treatment, stress reaction of the coreius guichenoti is reduced, simultaneously inflammation of the fish body is diminished, exogenous nutrient substances are supplemented appropriately, and wild coreius guichenoti damage caused by fishing and transportation is recovered as soon as possible. Culture survival rate of the collected coreius guichenoti under the artificial condition is increased, enough population for researching domestication and artificial reproduction technology of the coreius guichenoti is provided, mass production of the coreius guichenoti and artificial breeding and releasing become possible, and a purpose of protecting the coreius guichenoti resource is achieved.

An artificial reproduction method for Beiliuli clam includes such steps as choosing excellent parent clams, culturing them in pool in hanging mode, taking ova, putting the host fish in the water in a tub, attaching the glochidiums onto host fish, culturing the host fish in culture pool until the young clams is separated from the host fish, culturing the young clams in water surface in hanging mode, and culturing in square crate in shallow water.

The invention provides an artificial reproduction method for wild kelps. The artificial reproduction method particularly includes the steps: (1) selecting a proper wild kelp reproduction site; (2) preparing parent kelps; (3) preparing spore fluid; (4) preparing a seedling collection box and a transplantation network; (5) collecting seedlings; (6) temporarily rearing the seedlings in a workshop; (7) temporarily rearing the seedlings on the sea; (8) separating the seedlings; (9) cultivating artificial seedlings; (10) transplanting the seedlings on a seabed. The seedlings are collected and intermediately cultured by the aid of the seedling collection box and the transplantation network in special design, rapid reproduction of artificial reproduction seedlings is realized, the influence of variable seabed terrains is overcome, and large-area seabed reproduction is realized.

An artificial inducement method for spawning and breeding erythroculter ilishaeformis relates to an artificial inducement method for spawning and breeding fish. The method solves the problems of a low hatching rate and a low post-natal survival rate in the existing artificial reproduction method of the erythroculter ilishaeformis. The breeding method comprises the following steps: 1. culturing parent fish in a culture pond; 2. injecting an aphrodisiac agent into the parent fish twice so that the parent fish autogenously spawns; 3. transferring the fish eggs into a hatching barrel for hatching; and 4. transferring fish fry into an outdoor pond after opening an inflatable intestinal canal of a fish maw so as to culture young fishes. The artificial inducement method for spawning and breeding of the erythroculter ilishaeformis can help shorten a breeding period, greatly improve the survival rate and the hatching rate of the parent fish, and obviously improve the economic benefit.

The invention discloses a method for artificial reproduction of brachymystax lenok tsinlingensis. The method for artificial reproduction of the brachymystax lenok tsinlingensis comprises the steps that (A) mature and highly active brachymystax lenok tsinlingensis is selected to serve as a parent; (B) reinforced cultivation of the parent is conducted; (C) artificial spawning is conducted, wherein two dosages of artificial spawning medicine is injected into the parent for artificial spawning from the end of March to the middle of April; (D) semen collection is conducted manually, wherein the parent is bred in an artificial spawning pond after the artificial spawning medicine is injected into the parent, a water inlet pipe is erected at one end of the artificial spawning pond, and the abdomen of the brachymystax lenok tsinlingensis parent is pressed regularly so that semen collection can be achieved from the alive brachymystax lenok tsinlingensis parent; (E) artificial insemination is conducted, wherein after an ovum is rinsed with normal saline for fish is collected, seminal fluid of a milter is squeezed on the ovum, then mixing is conducted with a feather, andartificial insemination is conducted; (F) artificial incubation is conducted, wherein a fertilized ovum is washed with purified water and then is poured in an incubation groove for incubation, a porous dandruff comb is arranged on the bottom of the incubation groove, and when fry which can swim appears, the fry is moved out of an incubation device to be bred in a fish tank. The method for artificial reproduction of the brachymystax lenok tsinlingensis is easy to implement, operation is easy and convenient, the fertilization rate and the hatching rate are high, obtained fish offspring are healthy, the body sizes of the obtained fish offspring are even, the rate of survival is high, the utilization rate of fish bait is high, and the growing speed is high.

The invention discloses an artificial reproduction method of chalcalburnus chalcoides aralensis. The artificial reproduction method includes steps of rearing and selecting parent fishes, artificially spawning, inseminating and incubating. The fully-artificial reproduction method which is reasonable, high in operability and massive in production of fry of chalcalburnus chalcoides aralensis is provided, and by optimizing the parent-fish rearing technology and utilizing three different spawning medicines for artificial spawning and inseminating, spawning rate, roe fertility rate and fry incubation rate in parent fish reproduction are increased to 88.2%, 97.0% and 83.64% respectively. The artificial reproduction method is scientific, reasonable and high in operability, industrialized production can be realized by the artificial reproduction method, fry can be provided for large-scale breeding, and high economic benefits are increased.

The invention discloses a paramisgurnus dabryanus selective breeding method. Concretely, the method comprises the following steps that (1) 12 microsatellite molecular markers relevant to the growth properties are provided; (2) a parent group 1 is selected for electronic marking and DNA (deoxyribonucleic acid) extraction; (3) the molecular markers are adopted for carrying out genotype screening on the parent group 1, and in addition, a parent group 2 is selected; (4) the molecular markers are adopted for carrying out genotype screening on the parent group 2, and parent groups 3 and 4 with different homozygous genotypes are respectively built; (5) the parent groups 3 and 4 are used as breeding groups to be respectively subjected to artificial reproduction to obtain parent groups 5 and 6; (6) hybridization is carried out between the parent groups 5 and 6, and in addition, scaled reproduction is carried out. Compared with a conventional breeding method, the method has the characteristics of high speed, stability and reliability, the practicability is high, the cost is low, the accuracy is high, the influence due to factors such as environment is avoided, the relying on the phenotypic character authentication can be avoided, the breeding years are shortened, the breeding efficiency is improved, and great application values are realized.

The invention relates to an artificial maturing method for exopalaemon carinicauda in anestrous seasons, in particular to an artificial reproduction method for exopalaemon carinicauda in autumns and winters by means of regulating and controlling the temperature and the illumination cycle and enriching nutrients for the exopalaemon carinicauda. The artificial maturing method mainly includes controlling the temperature of water; regulating and controlling the illumination cycle; and enriching nutrients for the exopalaemon carinicauda. Temperature control equipment simulates a procedure that the temperature of water in springs rises gradually so as to control the temperature of the water; fluorescent lamps and a timer simulate a procedure that the illumination cycle is gradually prolonged in the springs so as to regulate and control the illumination cycle; and fresh baits such as nereid are fed to the exopalaemon carinicauda to enrich nutrient supplied for the exopalaemon carinicauda in a gonad development procedure. The exopalaemon carinicauda can smoothly reproduce by laying eggs after being bred for 2-3 weeks under the condition. The artificial maturing method has the advantages that characteristics that the temperature of the water gradually rises in the springs and the illumination cycle is gradually prolonged under natural conditions are simulated, so that gonads of the exopalaemon carinicauda are developed in the anestrous seasons, the exopalaemon carinicauda reproduces by laying the eggs in the anestrous seasons, and the purpose of controlling reproduction of the exopalaemon carinicauda throughout the year is achieved.

An artificial reproduction method for Triplophysa yarkandensis comprises the following steps of (1) preparation before production, (2) artificial production hastening and (3) artificial incubation. Pubescent Triplophysa yarkandensis individuals are selected, production hastening medicine and dosage are selected, the maturity of Triplophysa yarkandensis parent fishes can be centralized and synchronized through a series of technologies and methods of artificial production hastening and insemination, temporary rearing in a flowing water pool, stimulation of water flow and artificial incubation, the Triplophysa yarkandensis parent fishes can spawn in a centralized mode within a short time, the effect time is shortened, the fertilization rate and the incubation rate are improved, the egg laying amount is increased, breed conservation and proliferation of the Triplophysa yarkandensis in the Tarim River drainage basin are achieved, a solid foundation is laid for Tarim River fishery resource protection, proliferation and releasing of indigenous fishes and development and protection of the Triplophysa yarkandensis, and meanwhile the blank of artificial reproduction of Triplophysa yarkandensis fishes at home and abroad is filled up. Experiments prove that the fertilization rate of the artificial reproduction method for the Triplophysa yarkandensis reaches up to 95 percent, the incubation rate reaches up to 80 percent, the rate of emergence is above 75 percent, and the effect time is 6h to 12h.