Method for artificial reproduction of brachymystax lenok tsinlingensis

Abstract

The invention discloses a method for artificial reproduction of brachymystax lenok tsinlingensis. The method for artificial reproduction of the brachymystax lenok tsinlingensis comprises the steps that (A) mature and highly active brachymystax lenok tsinlingensis is selected to serve as a parent; (B) reinforced cultivation of the parent is conducted; (C) artificial spawning is conducted, wherein two dosages of artificial spawning medicine is injected into the parent for artificial spawning from the end of March to the middle of April; (D) semen collection is conducted manually, wherein the parent is bred in an artificial spawning pond after the artificial spawning medicine is injected into the parent, a water inlet pipe is erected at one end of the artificial spawning pond, and the abdomen of the brachymystax lenok tsinlingensis parent is pressed regularly so that semen collection can be achieved from the alive brachymystax lenok tsinlingensis parent; (E) artificial insemination is conducted, wherein after an ovum is rinsed with normal saline for fish is collected, seminal fluid of a milter is squeezed on the ovum, then mixing is conducted with a feather, andartificial insemination is conducted; (F) artificial incubation is conducted, wherein a fertilized ovum is washed with purified water and then is poured in an incubation groove for incubation, a porous dandruff comb is arranged on the bottom of the incubation groove, and when fry which can swim appears, the fry is moved out of an incubation device to be bred in a fish tank. The method for artificial reproduction of the brachymystax lenok tsinlingensis is easy to implement, operation is easy and convenient, the fertilization rate and the hatching rate are high, obtained fish offspring are healthy, the body sizes of the obtained fish offspring are even, the rate of survival is high, the utilization rate of fish bait is high, and the growing speed is high.

Description

technical field [0001] The invention belongs to the technical field of protection of germplasm resources of endangered wild animals, and specifically relates to an artificial breeding method for Qinling lentil salmon. The method is applicable to the natural population restoration and artificial conservation of Qinling salmon. Background technique [0002] Qinling fine scale salmon ( Brachymystax lenok tsinlingensis ), commonly known as flower fish, five-color fish, plum blossom fish, belonging to Saloniformes (Saloniformes), Salmonidae (Salmonidae), is a land-sealed cold-water fish endemic to my country and locally distributed. The distribution range of Qinling lentinus is extremely narrow, the habitat water conditions are strict, and the natural population is scarce. It is only distributed in the Heihe River at the east foot of the Taibai Mountain Peak in the Qinling Mountains, the Shitou River at the north foot, the Yushui River and Taibai River at the south foot, and the...

AI Technical Summary

Problems solved by technology

However, due to the serious miniaturization of Qinling leptospirosis, the sharp reduction of sexually mature resources, and harsh habitat conditions, Qinling leptospirosis is impatient, likes to jump and swims well, has less mucus on the body surface, thin scales on the body, and relatively small fish bones in the body. , strong stress and other characteristics, and its parents are prone to bruises and death (Shi Deliang et al., 2012)

In addition, Qinling salmon is a carnivorous cold-water fish, and the absolute number of fecundity of female fish is small; in the natural environment, the fertilization rate, hatching rate and survival rate are low, and the growth rate is slow, the growth cycle is long, and artificial domestication is difficult; It usually takes 3 to 4 years to mature, and the growth cycle of 500 g fish is generally 4 to 5 years, which increases the difficulty of restoring the germplasm resources of Qinling salmon.

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