33 results about "Brachymystax lenok" patented technology

The invention relates to the field of fry rearing, in particular to a brachymystax lenok fry high-density rearing method. The brachymystax lenok fry high-density rearing method comprises the steps of controlling the fry rearing density, controlling the water flow, controlling types of baits, controlling the feeding quantity and controlling the illumination intensity. According to the rearing method disclosed by the invention, high-density rearing can be carried out on the premise of controlling the survival rate of fries, rearing cost of brachymystax lenok is reduced, the output is improved, manual rearing of brachymystax lenok is implemented, and the current situation of shortage of resources of brachymystax lenok in China can be relieved.

An artificial inducement method for spawning of brachymystax lenok, relates to an artificial inducement method for spawning of fish, which solves the problems of unfocused spawning time, long spawning period and low hatching rate, comprising the steps of: 1. respectively delivering the parent fish into an induced spawning pond in spring; 2. performing induced spawning on the parent fish when water temperature reaches 7-12 DEG C; 3. injecting oxytocic in the pectoral fin basis free of squamae after the male and the female fish are hocused, adopting the secondary injection, then mixing the parent fish, after seven days, discharging seminal fluid by the male fish and ovulating by the female fish, namely manual induced spawning of brachymystax lenok. The invention causes spawning time to centralize, the spawning time is shortened by 10-15 days, and the hatching rate reaches 60%-70%; manual induced spawning is performed to breed the parent fish, the induced spawning success rate of the male fish reaches 100%, and the induced spawning success rate of the female fish reaches 40-50%; manual induced spawning is performed on wild parent fish, the induced spawning success rate of the male fish reaches 100%, and the induced spawning success rate of the female fish reaches 50-80%.

A method for rearing hybrid salmon seeds is used for solving the problems that presently, death rate of hybrid salmon seeds obtained by mating Brachymystax lenok with taimen is high, and techniques of rearing seeds of other salmon species is highly complex and difficult to industrialize and is unsuitable for rearing hybrid seeds of Brachymystax lenok and taimen. The method includes: placing fertilized eggs of hybrid salmon into sterilized parallel tanks, incubating the fertilized eggs in the shade till fry begin hatching, and removing egg skin, dead eggs and dead fry during hatching; and using water fleas as biological bait for feeding when hybrid salmon fry open mouths, using Tubificidae to feed the fry on the seventh day to the tenth day after mouth opening, mixing Tubificidae with complete feed into muddy feed for feeding, and using the complete feed to feed the fry. Hybrid salmon seeds reared by the method are high in quality, and the survival rate can be increased to more than 80%.

The invention discloses a method for artificial reproduction of brachymystax lenok tsinlingensis. The method for artificial reproduction of the brachymystax lenok tsinlingensis comprises the steps that (A) mature and highly active brachymystax lenok tsinlingensis is selected to serve as a parent; (B) reinforced cultivation of the parent is conducted; (C) artificial spawning is conducted, wherein two dosages of artificial spawning medicine is injected into the parent for artificial spawning from the end of March to the middle of April; (D) semen collection is conducted manually, wherein the parent is bred in an artificial spawning pond after the artificial spawning medicine is injected into the parent, a water inlet pipe is erected at one end of the artificial spawning pond, and the abdomen of the brachymystax lenok tsinlingensis parent is pressed regularly so that semen collection can be achieved from the alive brachymystax lenok tsinlingensis parent; (E) artificial insemination is conducted, wherein after an ovum is rinsed with normal saline for fish is collected, seminal fluid of a milter is squeezed on the ovum, then mixing is conducted with a feather, andartificial insemination is conducted; (F) artificial incubation is conducted, wherein a fertilized ovum is washed with purified water and then is poured in an incubation groove for incubation, a porous dandruff comb is arranged on the bottom of the incubation groove, and when fry which can swim appears, the fry is moved out of an incubation device to be bred in a fish tank. The method for artificial reproduction of the brachymystax lenok tsinlingensis is easy to implement, operation is easy and convenient, the fertilization rate and the hatching rate are high, obtained fish offspring are healthy, the body sizes of the obtained fish offspring are even, the rate of survival is high, the utilization rate of fish bait is high, and the growing speed is high.

The invention discloses an artificial hatch feed for brachymystax lenok fry, and relates to an artificial hatch feed for fry. The feed solves the problems that the conventional artificial hatch feed for the brachymystax lenok fry is high in labor intensity of workers, high in raising cost and difficult to breed large-batch fry. The artificial hatch feed for the brachymystax lenok fry consists of Peruvian fish meal, Norwegian fish meal, fish oil, soybean oil, soybean lecithin, whole egg powder, milk powder, chilled water earthworm powder, algae powder, betaine, cooked starch, multiple vitamins, mixed mineral salt, carboxymethylcellulose and vitamin E. By using the artificial hatch feed for the brachymystax lenok fry, the breeding survival rate and the weight of the fry are increased in a breeding period of 30 days and do not have remarkable difference compared with live bait for transition hatch breeding, the cost of the feed is reduced by over 60 percent, 50 percent of persons are saved, the cost of the feed is low, the labor intensity of the persons is reduced, hatch and domestication of the brachymystax lenok fry can be performed in batch, and a firm foundation is laid for breeding commercial fish in a large scale.

The invention discloses a method of promoting synchronous sexual maturation in male and female parents of Brachymystax lenok. The method includes 1, controlling the water temperature of a culture system by stages during parent fish rearing; 2, adjusting feed according to different growth stages of parent fishes. According to biology and ecological habits of the Brachymystax lenok, the method fully conforms to the environmental conditions of wild state in terms of water environment and feed structure by applying bionic technology during the period of parent fish domestication and cultivation; different feeds are used for strengthening cultivation during the different developmental stages, the water temperature and water flow are controlled, and accordingly ecological cultivation under artificial domestication is achieved; finally, the male and female parent fishes synchronously develop gonadally, and artificial spawning and fertilization can be performed smoothly.

The invention relates to a special feed for juvenile fishes of brachymystax lenok, and relates to a special feed for juvenile fishes. The invention solves the problem of low cultivation survival rate caused by no special specific feed in an existing process of cultivating the juvenile fishes of the brachymystax lenok. The special feed for the juvenile fishes of the brachymystax lenok is prepared by mixing fish meal, casein, bean pulp, beer yeast, corn gluten meal, wheat flour, fish oil, vitamin premix, mineral matter premix, choline chloride, soyabean lecithin, sodium carboxymethylcellulose and Ca(H2PO4)2. After the juvenile fishes of the brachymystax lenok are fed by using the feed provided by the invention, the cultivation survival rate is increased by 15-30 percent, and the average weight gain rate is increased by 140-220 percent; and the special feed for the juvenile fishes of the brachymystax lenok has the effects of complete nutrition, promoting the juvenile fishes of the brachymystax lenok to grow, increasing the immunity and the like, and is suitable for commercialized production. The special feed for the juvenile fishes of the brachymystax lenok can be used for feeding the juvenile fishes of the brachymystax lenok.

The invention relates to a brachymystax lenok parent fish nutrient enrichment dry-fresh composite feed and a preparation method thereof. The brachymystax lenok parent fish nutrient enrichment dry-fresh composite feed comprises the following components in percentage by weight: 46% of expanded feeds, 51% of fresh and alive baits, 2.1% of micronutrients and the balance being adhesives (0.9%). According to the feed and the method, the dry-fresh composite feed prepared by adding the fresh and alive baits to the expanded feeds is still a floatable feed, so that the requirement for life habits of a brachymystax lenok parent fish on that the brachymystax lenok parent fish only ingests the floatable feed is met; the contents of linolenic acid, linoleic acid, EPA (Eicosapentaenoic Acid) and DHA (Docosahexenoic Acid) which are contained in the dry-fresh composite feed can be increased by adding linseed oil and fish oil to the dry-fresh composite feed, so that the requirement for nutrition of the gonad development of the brachymystax lenok parent fish, the smoothness of spawning is facilitated, and the recovery of the postpartum parent fish is facilitated.

The invention relates to a Brachymystax lenok Cathelicidin antimicrobial peptide CATH_BARLE, and a gene, a preparation and an application thereof, and belongs to the technical field of biomedicine. The gene of the CATH_BARLE is composed of 909 nucleotides, and a part coding the mature peptide is nucleotides from the 439th bit to the 597th bit. The mature peptide CATH_BARLE is rich in alkaline amino acids, and has a strong bactericidal effect on common pathogens in aquatic product culture. The CATH_BARLE has a simple structure, does not contain a disulfide bond or an annular structure, and is convenient for chemical synthesis and gene engineering preparation; and the CATH_BARLE also has a good killing effect on many clinical drug-resistant bacteria, especially fungi.

The invention discloses an artificial breeding technology of brachymystax lenok and relates to the technical field of breeding of brachymystax lenok. The artificial breeding technology of the brachymystax lenok comprises steps as follows: 1, parent fish breeding; 2, artificial breeding; 3, postpartum treatment of parent fish; the minimum sexual maturity age of male wild brachymystax lenok is 4 years, the minimum sexual maturity age of female wild brachymystax lenok is 5 years, the brachymystax lenok at the age of 5 years old or younger has no egg laying behavior under the artificial breeding condition, the captured wild parent fish can perform breeding after completely adapting to man-made environment when the captured wild parent fish is domesticated and bred for at least one year, a flowing water cement pond is adopted as the parent fish breeding pond, and 4-5 pairs of brachymystax lenok are bred in every 10 m<2> of the flowing water cement pond. The delivery and breeding environmentof the brachymystax lenok is controlled through artificial breeding, so that female and male fish grow basically synchronously, smooth artificial breeding is guaranteed, the insemination success rateof the brachymystax lenok is increased, the growth and survival rate of the bred fish is increased, the death rate is greatly reduced, and thus, the artificial breeding effect of the brachymystax lenok is improved.

The invention discloses a traditional Chinese herbal medicine feed additive for preventing bacillary enteritis of brachymystax lenok and application of the traditional Chinese herbal medicine feed additive. The traditional Chinese herbal medicine feed additive comprises the following components in parts by weight: 16-20 parts of shepherd's purse, 15-18 parts of isatis roots, 13-18 parts of prepared rehmannia roots, 15-20 parts of Chinese violet, 10-13 parts of vervain, 8-12 parts of wild chrysanthemum flos, 10-13 parts of licorice roots, 8-13 parts of senecio scandens, 3-6 parts of equisetum hyemale and 5-8 parts of flower of operculate cleistocalyx. The traditional Chinese herbal medicine feed additive has the beneficial effects that the additive is a good 'green' medicinal additive, can be completely degraded, is capable of killing pathogene of bacillary enteritis, achieving the purpose of preventing bacillary enteritis of the brachymystax lenok, meanwhile stimulating the immunity system of the brachymystax lenok and improving the immunity of the brachymystax lenok; feed with the additive needs no other antibiotic medicine, so that accumulation of antibiotics in bodies of the brachymystax lenok is reduced, and thus requirements of modern people on foods are met.

The invention discloses a cultivation method of brachymystax lenok fry.The cultivation method includes the steps of A, performing artificial incubation; B, performing mouth-opening feeding on the fry; C, moving out of an incubation tank for cultivation, and stocking at reasonable density; D, domesticating in different ponds, to be more specific, selecting palatable baits, controlling feeding habits, and performing scientific daily management and disease prevention.The cultivation method has the advantages that completely artificial regulation is performed in the aspects of water temperature, light, dissolved oxygen and the like according to the biological features of brachymystax lenok, food intake regulation and standard management are used to allow the fry to be robust, even, high in environment tolerance, high in disease resistance, and the like, high bait utilization rate is achieved, the fertile egg hatchability and filial-generation fish growth rate and survival rate of the brachymystax lenok are increased greatly; filial-generation fish is robust, even, and high in environment tolerance and disease resistance, and the occurrence of malformation is reduced greatly; the method is simple, high in operability, good in practical application performance, completely suitable for the large-scale production of the fry, and applicable to species resource protection and natural population restoration of the brachymystax lenok.

The invention discloses a primer pair, reagent kit and method for distinguishing brachymystax lenok from Hucho taimen. The primer pair comprises a primer pair 1 and a primer pair 2, wherein the primerpair 1 comprises an upstream primer ITS-F and a downstream primer ITS-R, and the primer pair 2 comprises an upstream primer mtDNA-F and a downstream primer mtDNA-R; the sequence of the ITS-F is shownas SEQID NO.1, the sequence of the ITS-R is shown as SEQID NO.2, the sequence of the mtDNA-F is shown as SEQID NO.3, and the sequence of the mtDNA-R is shown as SEQID NO.4. A new method for distinguishing brachymystax lenok from Hucho taimen based on a molecular biology technique principle is adopted for the first time at home and abroad, 1 pair of specific primers is designed by using ITS genesand mitochondrial genome of the brachymystax lenok and the Hucho taimen, and a new method for identifying the brachymystax lenok and the Hucho taimen can be realized by a PCR reaction. The primer pair, the reagent kit and the method can be used for distinguishing the brachymystax lenok from the Hucho taimen complete individual and processed products thereof, and have the characteristics of being accurate, quick, objective, fair, economical and practical.

The invention relates to an artificial domestication method for Brachymystax lenok tsinlingensis. The method includes the steps of fishing, temporary rearing, domestication, and culture. By means of the method, the domestication success rate of Brachymystax lenok tsinlingensis can be increased to 90% or above, the domestication time can be shortened by 6 months, and the growth speed of wild Brachymystax lenok tsinlingensis in the place of origin is increased by 3-5 times after successful domestication.

The invention discloses a microbial water purifier for improving water quality for Brachymystax lenok and purpose thereof. A preparation method of the water purifier specifically comprises the following steps: putting potatoes in boiling water to cook, filtering, adding glucose and agar into a filtrate, and sterilizing to obtain a PAD culture medium; taking various ingredients according to formularatio of the culture medium, adding water and uniformly mixing, carrying out pressure maintaining treatment, and cooling to obtain a culture medium; inoculating an activated strain into the PAD culture medium and culturing to obtain a seed solution; and inoculating the seed solution and culturing to obtain the microbial water purifier. By adding 15-25 ML of the water purifier into each cubic meter of aquaculture water, effects of nitrification, denitrification, nitrogen reduction, phosphorus removal, removal of organic matter in water and the like can be realized; eutrophication materials such as nitrogen, phosphorus, etc. in water can be greatly removed; other harmful bacteria in a water body can be effectively inhibited; the effects of preventing diseases and raising survival rate are achieved; and the purpose of purifying a water body is achieved.

The invention relates to a fry breeding method of tetraploid fry of brachymystax lenok. The method comprises the following steps of firstly, performing in-vitro fertilization on eggs and sperms of parent brachymystax lenok; then, treating the fertilized eggs by means of a heat shock method; then, carrying out temperature change treatment on the fertilized eggs; finally, incubating the fertilized eggs in water for breeding, and performing culturing to obtain the tetraploid fry of brachymystax lenok. According to the method, the preparation of the tetraploid fry of brachymystax lenok is realizedby adopting an in-vitro fertilization technology for female and male brachymystax lenok and the heat shock method, the eyed rate, fry hatchability rate and tetraploid rate of the fertilized eggs areeffectively increased, and a stable triploid brachymystax lenok population can be obtained through hybridization with normal diploid brachymystax lenok, so that the problems of sexual prematurity, individual miniaturization and the like of bred individuals, which are caused by long-term inbreeding and lack of systematic breeding in a brachymystax lenok breeding process, are solved. The method increases the specification and growth speed of commercial fishes obtained through brachymystax lenok breeding and production and has good economic benefits and a high market value.

The invention discloses a mixed feed additive containing vitamin C and Chinese herbal medicines and capable of improving brachymystax lenok immunity, and applications thereof. The additive comprises the following components in parts by weight: 19-24 parts of honeysuckle, 13-17 parts of rheum officinale, 18-25 parts of milk-vetch roots, 5-7 parts of radix angelicae sinensis, 16-20 parts of Chinese yams, 9-11 parts of Chinese thorowax roots, 6-10 parts of manyprickle acanthopanax roots, 13-17 parts of common andrographis herb, 2-4 parts of garlic, 3-5 parts of dried orange peel, 3-4 parts of codonopsis roots, 3-5 parts of poria cocos, and 1-2 parts of vitamin C. The beneficial effects of the invention are that the components of the additive are very convenient to obtain and low in price; the additive has an effect of adjusting immune organs and immune cells so as to prevent microbes out of the cells and toxins of the microbes from harming organisms, and to improve immunity and disease resistance of the brachymystax lenok; and a preparation method of the additive is simple, safe, energy-saving, pollution-free, simple in production equipment, and investment-saving.

The invention discloses an identifying primer and identifying method for germplasm of hucho taimen and brachymystax lenok and a reagent kit. The primer comprises an upstream primer HtaC1041F and a downstream primer HtaC1041R, wherein the sequence of the upstream primer HtaC1041F is as shown in SEQID NO.1, and the sequence of the downstream primer HtaC1041R is as shown in SEQID NO.2. The identifying method disclosed by the invention a new method for identifying the germplasm of the hucho taimen and the brachymystax lenok, established based on a molecular biology technique principle for the first time at home and abroad, a pair of SSR primers are designed through an EST sequence of the hucho taimen, and through a PCR reaction, identification of the hucho taimen and the brachymystax lenok canbe realized. Through the adoption of the identifying primer and the identifying method disclosed by the invention, complete individuals and processed products of the hucho taimen and the brachymystaxlenok can be distinguished, and besides, the identifying primer and the identifying method can be used for identification of roes and offspring which is justly incubated, and have the characteristicsof being accurate, quick, objective, fair, economical and practical.