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Method for measuring curcumin content in fish feed

A breeding method and seedling technology, applied in the field of analysis and testing, can solve the problems of no bis-demethoxy curcumin, etc., and achieve the effects of short time, less consumption of reagents, and good reconstitution effect

Inactive Publication Date: 2016-06-15
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports or patent applications for the determination methods of bis-demethoxycurcumin, demethoxycurcumin and curcumin in fish feed at home and abroad

Method used

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  • Method for measuring curcumin content in fish feed
  • Method for measuring curcumin content in fish feed
  • Method for measuring curcumin content in fish feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The present invention will be further described below in conjunction with the accompanying drawings.

[0039] A method for measuring curcumin in fish feed, its step is

[0040] A. Take 5.0g of fish feed into a 50mL plastic centrifuge tube, add 15-30mL of reagent A, shake for 30s, ultrasonicate for 5min, put the 50mL plastic centrifuge tube on a centrifuge at 7000r / min, and centrifuge for 5min;

[0041]B. Transfer the upper extract to a 125mL rotary concentrator bottle, then add 15-30mL of reagent A to the 50mL test tube residue to repeat the extraction once, place the rotary concentrator bottle on a rotary evaporator at 40°C to concentrate until dry, and add to the rotary concentrator bottle Reagent B1~10mL Vortex for 30s to redissolve the extract residue;

[0042] C. Transfer the reconstituted solution obtained in step B to a 10mL plastic centrifuge tube, blow nitrogen on a 40°C nitrogen blower until dry, then add 1mL volume-constant solution C to the 10mL plastic cen...

Embodiment 2

[0062] A method for measuring curcumin content in fish feed, the steps are:

[0063] A. Take 5.0g of fish feed into a 50mL plastic centrifuge tube, add 15-30mL of reagent A, shake for 30s, and sonicate for 5min;

[0064] The reagent A can be any liquid reagent in ethyl acetate, acetone, acetonitrile, methanol, ethanol, dichloromethane, and n-hexane.

[0065] B. Put the centrifuge tube on a centrifuge at 7000r / min, centrifuge for 5min, transfer the upper layer extract to a 125mL rotary concentrator bottle, then add 15-30mL of reagent A to the 50mL test tube residue and repeat the extraction once; the reagent A can be acetic acid Any liquid reagent in ethyl ester, acetone, acetonitrile, methanol, ethanol, dichloromethane, n-hexane.

[0066] C. Put the rotary concentration bottle on a 40°C rotary evaporator to concentrate to dryness, add reagent B1-10mL to the rotary concentration bottle, vortex and shake for 30s to redissolve the residue in the rotary concentration bottle; the ...

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Abstract

The invention discloses a cultivation method of brachymystax lenok fry.The cultivation method includes the steps of A, performing artificial incubation; B, performing mouth-opening feeding on the fry; C, moving out of an incubation tank for cultivation, and stocking at reasonable density; D, domesticating in different ponds, to be more specific, selecting palatable baits, controlling feeding habits, and performing scientific daily management and disease prevention.The cultivation method has the advantages that completely artificial regulation is performed in the aspects of water temperature, light, dissolved oxygen and the like according to the biological features of brachymystax lenok, food intake regulation and standard management are used to allow the fry to be robust, even, high in environment tolerance, high in disease resistance, and the like, high bait utilization rate is achieved, the fertile egg hatchability and filial-generation fish growth rate and survival rate of the brachymystax lenok are increased greatly; filial-generation fish is robust, even, and high in environment tolerance and disease resistance, and the occurrence of malformation is reduced greatly; the method is simple, high in operability, good in practical application performance, completely suitable for the large-scale production of the fry, and applicable to species resource protection and natural population restoration of the brachymystax lenok.

Description

technical field [0001] The invention belongs to the field of analysis and testing, and more specifically relates to a UPLC-TUV method for determining the content of bisdemethoxycurcumin, demethoxycurcumin and curcumin in fish feed, which is suitable for determining the content of bisdemethoxycurcumin in fish feed The content of oxycurcumin, demethoxycurcumin and curcumin. Background technique [0002] Curcumin, also known as curcumin, includes bis-demethoxy curcumin, demethoxy curcumin and curcumin. It has the pharmacological properties of protecting intestinal mucosa, anti-oxidation, anti-inflammation, antibacterial, lowering blood fat, protecting liver, and choleretic Activity, the physiological activities of the three components are also different, the antioxidant activity of curcumin is greater than that of demethoxycurcumin and bisdemethoxycurcumin, and the activity of demethoxycurcumin for lowering blood lipids is significantly higher than that of curcumin. Bis-demeth...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/12A01K61/00
CPCA01K61/00G01N30/02G01N30/06G01N30/12G01N2030/126Y02A40/81
Inventor 刘永涛艾晓辉杨红董靖胥宁杨移斌杨秋红
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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