The invention discloses a closed automatic detection device for blood rheology measurement, which comprises a test tube stand splint, a bar code scanner, a test tube stand moving device, an automatic blood sample transfer device of a test tube stand position monitor, a sampling measurement system comprising a sampling/measuring needle assembly, a needle insertion driving device, a quantitative sampling controller, an upper sphere, a heating module, a washing device and a work station conversion driving device, a blending device comprising an elastic clamping device, an elastic clamping device autorotation driving motor, an up-down moving device and a horizontal moving device, an air pump, a singlechip and the like. The device can ensure that the blood rheology measurement realizes the automatic transfer of a blood sample, automatic reversal of the blending device of the blood sample, automatic quantitative sampling, automatic measurement of the blood sample, automatic washing of a measurement device, and automatic recognition and recording under the condition of not opening a cover, realize the automatic measurement of blood viscosity under the full-closed state, can avoid the cross contamination, can also alleviate the labor intensity of the staff, improve measurement effect, and can also shorten measurement time.