31 results about "Quechers" patented technology

The invention discloses a multi-dimensional high-precision detection method and system for biotoxins in agricultural products, and the method comprises the steps: carrying out sterile collection and grading pretreatment on an agricultural product sample to obtain a homogenized sample; extracting the homogenized sample by adopting a mixed solvent system, adding an internal standard substance, carrying out salting-out layering, and collecting supernate; sequentially carrying out QuEChERS purification, multi-mechanism adsorption column purification and molecularly imprinted polymer specific enrichment on the supernate; carrying out dual-mode detection on the target toxin enrichment liquid, respectively carrying out quantitative detection by adopting a liquid chromatography-tandem mass spectrometry method, and carrying out screening detection by adopting an intelligent electrochemical sensing method; performing optimization processing on the dual-mode detection signal, and comprehensively judging a sample detection result; through integrated application of a triple purification mechanism, a dual-mode detection system, machine learning signal analysis and a full-chain quality control technology, efficient enrichment and deep interference removal of target toxins in a complex matrix can be realized, and both precision and applicability are considered.

This invention discloses an integrated pretreatment tube and its working method for detecting mycotoxins in camellia milk. The pretreatment tube includes an outer tube with an outer tube cap at its top, a middle tube inside the outer tube with its top protruding from the outer tube cap and a middle tube cap at its top, and an inner tube inside the middle tube with its top protruding from the middle tube cap and its top cap. An ultrafiltration tube is located inside the inner tube, with its outer sidewall conforming to the inner sidewall of the inner tube, and its bottom forming an ultrafiltration element. At least one set of micropores is provided on the sidewall of the middle tube, and a first microporous filter membrane covers these micropores. At least one set of micropores is also provided on the sidewall of the inner tube, and a second microporous filter membrane covers these micropores. Through holes are provided on the sidewall of the ultrafiltration tube, and these through holes are directly opposite the positions of the second microporous filter membrane. A QuEChERS reagent pack is located at the bottom of the middle tube, and a salt pack is located at the bottom of the outer tube.

The invention discloses a QuEChERS-high performance liquid chromatography determination method for diarrheogenic illegal additives in food, and the method comprises the following steps: step 1, taking 2.0 g of a food sample, adding 10 mL of an extraction solvent, carrying out vortex oscillation-ultrasonic treatment, then adding anhydrous magnesium sulfate and sodium chloride, carrying out vortex mixing, carrying out centrifugal treatment, and extracting a supernatant; 2, taking 5mL of the supernate, adding the supernate into a purification tube containing purification salt, carrying out vortex oscillation-centrifugal treatment, taking the supernate, and enabling the supernate to pass through a 0.45 mu m polytetrafluoroethylene microfiltration membrane, so as to obtain a solution to be detected; and 3, injecting the solution to be detected into a high performance liquid chromatograph, carrying out gradient elution by adopting a C18 chromatographic column and taking acetonitrile-water as a mobile phase at a flow rate of 1.0 mL / min, a column temperature of 30 DEG C, a detection wavelength of 210 nm and a sample size of 10 [mu] L, and quantitatively analyzing a target compound through an external standard method. The invention aims to obtain a method for detecting illegal additives in food, which is low in cost and high in detection efficiency.

The invention discloses a non-targeted screening method for pesticide and veterinary drug residues in raw milk based on LC-TOFMS (liquid chromatography-time of flight mass spectrometry) and application of the non-targeted screening method, and belongs to the technical field of food safety detection. According to the method, a QuEChERS technology is adopted to carry out pretreatment on a raw milk sample, a liquid chromatography-time-of-flight mass spectrometry technology is utilized to carry out detection, and a self-built mass spectrometry database is combined to realize non-targeted qualitative screening. A self-established mass spectrum database comprises precise mass numbers, retention time, parent ion loading modes and secondary mass spectrum fragment information of 46 kinds of pesticides and 54 kinds of veterinary drugs. The method is simple in pretreatment, high in detection speed, wide in coverage range and suitable for rapid screening and quality monitoring of pesticide and veterinary drug residues in raw milk, and has good practicability and popularization value.

The invention belongs to the technical field of pesticide residue detection, and relates to a method for detecting residues of four herbicides in farmland soil through QuEChERS pretreatment-high performance liquid chromatography-tandem mass spectrometry. The method comprises the following specific steps: (1) drawing a standard curve: preparing a mixed standard solution of four pesticides including topramezone, bicyclopyrone, isoxaflutole and dicarfentrazone, carrying out high performance liquid chromatography-tandem mass spectrometry analysis, and drawing the standard curve according to the concentrations of the four pesticide standard solutions and quantitative ion pair peak areas; (2) QuEChERS pretreatment: performing solvent extraction, salt extraction and purifying agent treatment on to-be-detected soil to obtain a to-be-detected sample solution; and (3) analyzing the concentration of the pesticide residues: performing high performance liquid chromatography-tandem mass spectrometry analysis on the sample solution to be detected, and calculating to obtain the concentration of the four pesticide residues in the soil to be detected. The method is simple in pretreatment, high in detection recovery rate, good in detection result repeatability and high in detection sensitivity.

The application discloses a pesticide detection method for rice based on QuEChERS and ultraviolet-visible spectrophotometry, and takes three pesticides, i.e., bensulfuron-methyl, propanil and cypermethrin, as examples to carry out research on a rapid screening method for multiple pesticide residues in rice. The improved QuEChERS method is used to process rice to obtain a matrix liquid, matrix standard solution and solvent standard solution are prepared, the quantitative linear range of each pesticide and the matrix effect are obtained. In the quantitative concentration range, mixed solutions of the three pesticides in four combinations are prepared, the types of the pesticides in the rice are determined through support vector machine qualitative analysis, and the concentrations of the pesticides are predicted through partial least squares regression analysis, so that the pesticide residues in the rice can be effectively and rapidly detected.

The invention discloses an FM550 in-vivo enrichment detection method and a risk monitoring method for tilapia, and relates to the technical field of ecological monitoring. The method not only can reflect the actual risk of a flame retardant in the environment, but also can be conveniently applied to aquaculture and ecological risk assessment. The detection method comprises the following steps: obtaining a liver sample and a muscle sample of a target tilapia mossambica, freeze-drying the liver sample and the muscle sample for 72 hours, and crushing and homogenizing 1 g of the liver sample and the muscle sample for later use; the target tilapia mossambica refers to tilapia mossambica which is exposed in an FM550 environment within 56 days; and carrying out solvent extraction, QuEChERS purification treatment and solvent conversion treatment on the crushed and homogenized sample, and then carrying out gas chromatography-mass spectrometry detection to obtain a detection result of FM550 in-vivo enrichment.

The invention discloses a method for determining N-nitrosamine compounds in animal-derived food, and belongs to the field of food detection. The method comprises the following steps: freezing and grinding animal-derived food, mixing the animal-derived food with an internal standard solution, deionized water and acetonitrile, and freezing and preserving to obtain a pretreated sample; the method comprises the following steps: treating a pretreated sample through a QuEChERS salt bag, centrifuging, carrying out nitrogen blowing concentration, and mixing with deionized water to prepare an enriched solution; purifying the enriched solution by adopting a purification bag or specific functional resin, and centrifugally filtering to obtain a sample solution; and calculating the content of the target compound through a peak area ratio by using a liquid chromatograph-high resolution mass spectrometer provided with an APCI source and taking N-nitrosodimethylamine-D6 as an internal standard. The functional resin is formed by cross-linking specific functional monomers and is large in specific surface area and high in adsorption selectivity; the method has strong anti-interference capability, and can efficiently detect the N-nitrosamine compounds in dried aquatic products and other animal-derived foods.

The invention relates to a method for determining halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl in food. The method is simple, convenient and rapid to operate, good in precision and recovery rate, and suitable for confirmed quantitative determination of halauxifen-methyl, prothioconazole and metabolite thereof in food.

The utility model relates to an efficient purification device based on a QuEChERS method, and belongs to the technical field of sample pretreatment, the efficient purification device comprises a purification pipe filled with an adsorbent, the bottom of the purification pipe is provided with a microfiltration membrane, and the purification pipe is detachably provided with a piston rod. The method has the advantage of improving the sample purification efficiency.

The invention belongs to the technical field of analytical chemistry and food / traditional Chinese medicine safety detection, and particularly relates to a magnetic dispersive solid-phase extraction material and a preparation method and application thereof. The preparation method of the magnetic dispersion solid-phase extraction material comprises the following step: preparing the magnetic dispersion solid-phase extraction material Fe3O4 (at) MWCNT (at) Uio-67 from a Fe3O4 (at) MWCNT magnetic composite material by adopting an in-situ growth method. The material has ultrahigh specific surface area, rich adsorption sites and good magnetic separation characteristics, can be used in a QuEChERS pretreatment method, realizes rapid, sensitive and accurate detection of mycotoxin based on an ultra-high performance liquid chromatography-tandem mass spectrometry analysis method, and provides reliable technical support for quality safety control of related products.

The application discloses a kind of detection methods of nitrofurans metabolites in macrobrachium rosenbergii based on UPLC-MS / MS and application thereof, belong to aquatic product safety evaluation detection technical field.The application utilizes QuECHERS method to the pretreatment of macrobrachium rosenbergii sample, extracts nitrofurans metabolites, including adding acidified acetonitrile extraction, adding ammonium acetate, water removal and adding dispersed solid extraction column to reduce matrix effect and other steps.After the QuECHERS method of the application is handled, the obtained nitrofurans metabolites do not need to be subjected to derivatization procedure, can directly use HILIC hydrophilic chromatographic column, in positive ion mode liquid chromatography-mass spectrometry analysis, detect aminourea and other nitrofurans metabolites.The detection method provided by the application has the advantages of high sensitivity, strong accuracy, wide applicability, compared with traditional detection method, can avoid reagent waste, save a lot of time.

The invention discloses a method for detecting malachite green based on a fluorescence resonance energy transfer principle, a biosensor and a kit, and belongs to the technical field of aquatic product quality safety detection. According to the method, near-infrared light excited lanthanide doped up-conversion nanoparticles (UCNPs) are used as an energy donor, and a nucleic acid aptamer for specifically recognizing malachite green is covalently linked to the surface of the UCNPs to form the nano probe. When the malachite green does not exist in the sample, the aptamer is in a flexible conformation, and the fluorescence of the UCNPs is quenched due to the FRET effect. When malachite green exists in a sample, the aptamer is specifically combined with the malachite green and is subjected to conformational change, so that the spatial distance between the nanoprobe and a quenching agent is increased, the FRET process is blocked, and UCNPs fluorescence is recovered. The qualitative and quantitative analysis of malachite green can be realized by detecting the change of a fluorescence signal. The method has the advantages of high sensitivity, strong specificity, simple operation and rapid response, and is suitable for on-site rapid detection of malachite green residues in aquatic products and aquatic water.

The invention discloses a preparation method of a porous carbon nitride material and application of the porous carbon nitride material as a QuEChERS purification adsorbent, and belongs to the technical field of pesticide residue detection.The preparation method comprises the following steps that 1, a nitrogen-containing precursor and nano calcium carbonate are mixed and stirred in absolute ethyl alcohol and then transferred into a crucible, drying is conducted, and a solvent is volatilized; (2) calcining the obtained solid in a tubular furnace at high temperature to prepare a porous carbon nitride frame structure; (3) stirring the frame structure of the porous carbon nitride in hydrochloric acid, removing the calcium carbonate template, washing and drying to obtain a porous carbon nitride precursor; and (4) calcining the porous carbon nitride precursor in a tubular furnace at high temperature, washing and drying to prepare the porous carbon nitride material. When the porous carbon nitride material is used for detecting pesticide residues of agricultural products such as vegetables and fruits, operation is easy and convenient, the purification effect is better than that of classical purification materials such as PSA, C18 and GCB, and the detection accuracy can be effectively improved.

The invention relates to a method for determining five ultra-short chain perfluorinated compounds in agricultural products, which comprises the following steps: S1, weighing 1.00-5.00 g of homogeneous sample in a first centrifuge tube, adding a certain amount of internal standard and 1-5 mL of ultrapure water, and carrying out vortex mixing for 1 min; s2, continuing to add 10 mL of 2% formic acid acetonitrile, homogenizing at a high speed for 1 min, adding a QuEChERS pretreatment bag, performing rapid vortex mixing uniformly, and performing high-speed centrifugation for 5 min; s3, transferring the acetonitrile on the upper layer into a second centrifugal tube filled with a purifying agent, carrying out full vortex, and carrying out high-speed centrifugation for 5 minutes; s4, 5 mL of supernate is taken and put into a third centrifugal tube, nitrogen blowing is performed at the temperature of 45 DEG C until the supernate is dry, 1 mL of methanol water with the volume ratio being 1: 1 is added for constant volume, centrifugation is performed at the high speed of 12000 r / min for 10 min, and supernate is sucked and passes through a 0.22-micron filter membrane; and S5, detecting the supernate passing through the filtering membrane in the step S4 by liquid chromatography-tandem mass spectrometry. Due to the adoption of the technical scheme, the method for determining the five ultra-short-chain perfluorinated compounds in the agricultural product can be used for simultaneously determining the five ultra-short-chain perfluorinated compounds in the agricultural product.

This invention discloses the application of isohypericin in the preparation of a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*, thereby reducing the toxicity of pathogens without inhibiting their growth. This invention provides the application of isohypericin in the preparation of a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*. It offers a novel solution for developing highly efficient and safe food preservatives.

The invention discloses an electrochemical luminescence (ECL) sensor based on a luminous hydrogel and graphene oxide (GO)-aptamer interface and an acetamiprid detection method thereof. The luminescent hydrogel is formed by loading manganese dioxide nanosheets (MnO2NSs), N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and gold nanoparticles (AuNPs) grown in situ in a three-dimensional network of polyethyleneimine-glutaraldehyde (PEI-GA) together, and is used as an ECL luminescent layer with high density and stable microenvironment; a GO-aptamer composite layer is constructed on the surface of the GO-aptamer composite layer, and signal transduction of GO short-range quenching-target induced desorption-signal recovery (signal-on) is achieved. An ECL signal is collected in a buffer system containing a hydrogen peroxide co-reactant, and a quantitative relation is established by delta IECL or I / I0, so that sensitive detection of acetamiprid is realized; good linearity is realized in a range of 1.0 * 10 <-3 > to 1.0 * 10 < 3 > nM, and the detection limit is about 0.3 pM. The method is good in repeatability and stability, and can be combined with QuEChERS pretreatment to be used for sample detection of vegetables such as spinach, celery and leek.

The application discloses a kind of 3-nitropropionic acid residues in sugar cane and sugar cane juice Gas Chromatography-Tandem Mass Spectrometry detection method.The application uses acetyl chloride-methanol method, acetyl chloride and methanol are reacted to obtain hydrochloric acid-methanol, which makes 3-nitropropionic acid in sugar cane and sugar cane juice carry out methyl esterification derivatization reaction, and 3-nitropropionic acid methyl ester is generated.After extraction with toluene and centrifugation, the upper liquid is added to the QuEChERS extraction bag, vortexed for 1 min, centrifuged at 10000 rpm for 5 min, and then the upper liquid is filtered through a 0.22 μm filter membrane.The sample is detected by Gas Chromatography-Tandem Mass Spectrometry in multiple reaction monitoring mode, and quantified by external standard method.The method has high extraction efficiency and sensitivity, good accuracy and repeatability, and can be used to determine the residue amount of 3-nitropropionic acid in sugar cane and sugar cane juice.

The invention relates to the technical field of detection, and particularly discloses a high-throughput screening and quantifying method for anesthetics in aquatic products based on high-resolution mass spectrometry, which comprises the following steps: constructing a mass spectrometry database containing information of at least 72 anesthetic compounds; the method comprises the following steps: carrying out pretreatment on an aquatic product sample by adopting an improved QuEChERS method; carrying out analysis and acquisition under specific chromatography and mass spectrometry conditions by utilizing an ultra-high performance liquid chromatography-electrostatic field orbitrap high-resolution mass spectrometer; the method comprises the following steps: acquiring data by adopting a Full MS-ddMS2 scanning mode; and finally, analyzing the data through a'non-targeted discovery-targeted confirmation 'strategy, namely, firstly performing non-targeted screening by using Compressed Discovery software, and then performing targeted confirmation and quantification on the basis of the mass spectrum database through TraceFinder software, the method is high in flux, good in sensitivity and strong in anti-interference capability, and the method is suitable for large-scale popularization and application. Various types of anesthetic residues in aquatic products can be rapidly and accurately screened, identified and quantified, and the defects of an existing standard detection method are overcome.

The invention relates to the technical field of cosmetic detection, and discloses a liquorice plant-derived cosmetic quality evaluation method. According to the method, through four core dimensions of liquorice source authenticity evaluation, 37 kinds of pesticide residue detection, 37 kinds of element detection and 6 kinds of anti-infection illegal additive substance detection, an optimized pretreatment process and an accurate detection technology are combined, and comprehensive and efficient evaluation on the quality of the liquorice plant-derived cosmetics is achieved. According to authenticity evaluation of the liquorice source, nine characteristic components are detected by LC-MS, a QuEChERS combined GC-MS / MS method is adopted for pesticide residue detection, a super microwave digestion-ICP-MS method is adopted for element detection, and a Raman spectrum combined nanogold sol enhancement technology is adopted for illegal addition detection; and through four-dimensional weighted scoring (total score is 100), if the score is greater than or equal to 80, the product is qualified. According to the method, the defects that an existing detection method is insufficient in pertinence, large in interference and narrow in coverage range are overcome, and systematic and reliable technical support is provided for quality control, efficacy verification and safety guarantee of licorice plant-derived cosmetics.

This invention provides a method for detecting brassinolide in microbial inoculants based on nanomaterials QuEChERS-UPLC-MSMS, using specific amounts of nano-ZrO2, PSA, and C. 18 Using MWCNTs as a purification agent combination for sample pretreatment, and with 0.1% formic acid aqueous solution and methanol as the mobile phase, under specific gradient elution conditions, the content of brassinolide in microbial inoculant samples can be accurately analyzed. The method of this invention has advantages such as high sensitivity, strong versatility, high accuracy, good stability, and simple operation, providing technical support for the detection of plant-derived pesticides from microbial inoculants.

This application relates to the field of analytical testing technology, specifically to a method for detecting pentachlorophenol (PCP) in animal-derived foods. The method includes providing 2g-10g of a sample from an animal-derived food source; alkalizing and extracting the sample using ammonia and acetonitrile to obtain a first mixture; adding a salting-out agent to the first mixture, shaking to disperse, then vertically oscillating for 2-5 minutes, followed by centrifugation at 3000-4000 rpm for 2-5 minutes; taking 2mL of the supernatant, adding it to a purification tube filled with packing material, mixing thoroughly, and filtering using a 0.22µm organic filter membrane to obtain the test solution; and performing LC-MS / MS detection using the test solution. This method optimizes the pretreatment process of the QuEChERS method while ensuring PCP extraction rate, thereby improving the efficiency and reducing the cost of PCP detection in animal-derived foods.

The invention discloses a method for measuring the residual quantity of prometryn in laver, which comprises the following steps: accurately weighing a sample, adding an acetonitrile chromatographic pure solution, vortex for 2 minutes, then adding a QuEChERS salt bag, oscillating for 5 minutes, centrifuging for 5 minutes at the rotating speed of 4000 rpm, and collecting supernate; 5 mL of supernate is taken and transferred into a purification pipe, vortex is carried out for 2 min, and centrifugation is carried out for 5 min at the same rotating speed; sucking 2mL of centrifuged purified liquid by using a pipette, and blowing the purified liquid by nitrogen until the purified liquid is nearly dry; fixing the volume to 2mL by using ethyl acetate chromatographic purity, extracting supernate by using an injector, passing through an organic filter membrane of 0.22 mu m to obtain sample liquid to be detected, and analyzing by using gas chromatography-triple quadrupole tandem mass spectrometry; the method has the advantages that the method is convenient to operate and high in sensitivity, and a new effective solution is provided for detection of prometryn in laver.

The invention relates to the field of food detection materials, in particular to a QuEChERS pretreatment composite adsorbent and a preparation method and application thereof.The QuEChERS pretreatment composite adsorbent comprises a) a magnetic core-dielectric layer composite structure which comprises a magnetic core composed of iron oxide nanoparticles and a dielectric layer wrapping the magnetic core; b) an ordered mesoporous oxide layer coated outside the magnetic core-dielectric layer composite structure; and c) the inner surface of the mesoporous oxide layer has hydrophobic groups and acidic functional groups through step-by-step modification. Through cooperative protection of the magnetic core and the functional layer, graded capture and accurate removal of interferents such as fat and protein are achieved, and meanwhile the rapid recovery capacity of the material in a complex matrix is enhanced.

The application discloses a method for synchronous detection of multiple mycotoxins. After crushing the sample to be detected, the sample is pretreated by using a hierarchical QuEChERS extraction method and / or a solid-phase extraction method according to the characteristics of the matrix to prepare a sample extract. A mixed control standard working solution containing aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, citrinin, ochratoxin A and zearalenone is prepared, and the seven mycotoxins are detected by using ultra-high performance liquid chromatography-tandem mass spectrometry. Qualitative and quantitative analysis is realized by alternately scanning positive and negative ions and multi-reaction monitoring. The method can be adapted to the need for mycotoxin control of traditional Chinese medicine decoction pieces, does not need an immunoaffinity column, has high pretreatment efficiency, low detection cost and high throughput, can realize synchronous and accurate detection of multiple toxins, and meets the demand for rapid screening of large batches of traditional Chinese medicine decoction pieces.

The application relates to the field of food detection materials, in particular to a QuEChERS pretreatment composite adsorbent as well as a preparation method and application thereof. The QuEChERS pretreatment composite adsorbent comprises: a) a magnetic core-dielectric layer composite structure which comprises a magnetic core composed of iron oxide nanoparticles and a dielectric layer coated outside the magnetic core; b) an ordered mesoporous oxide layer coated outside the magnetic core-dielectric layer composite structure; and c) the inner surface of the mesoporous oxide layer is provided with hydrophobic groups and acidic functional groups through step-by-step modification. Through the synergistic protection of the magnetic core and the functional layer, the application realizes the hierarchical capture and accurate removal of interfering substances such as fat and protein, and meanwhile, the rapid recovery capacity of the material in a complex matrix is strengthened.

The application provides a magnetic QuEChERS purifier and application thereof in pollutant detection, and the purifier is a MIL-88A derived carbon material which is prepared by high-temperature carbonization of a MIL-88A precursor, has a large specific surface area, rich pores, various surface functional groups and superparamagnetism, is used in the purification step of QuEChERS pretreatment, can efficiently adsorb and remove humic acid and other matrix interference substances in soil / sediment extract, has weak adsorption to target pollutants BPs, and can realize rapid separation in combination with an external magnetic field. The detection method established based on this has the advantages of low matrix effect, high recovery rate, simple and fast operation, and improves the accuracy and reliability of pollutant detection in soil / sediment.

The invention provides a method for separating and detecting phthalic acid ester substances, which comprises the following steps: S1, putting a proper amount of sample into a heat-resistant glass tube, adding enough QuEChERS salt bag without plasticizer authentication, manually oscillating the glass tube until the mixture is fully mixed, standing until the mixture is obviously layered, carefully sucking supernate and transferring the supernate into a clean sample injection bottle, according to the present invention, the traditional SPE step is replaced by the online purification, the pretreatment process is optimized, the manual operation and the vessel use are reduced, the activation, the concentration and other time-consuming links are omitted, the plastic-free reagent and the liner tube adsorption design are combined, the background pollution is significantly reduced, and the efficient and convenient sample pretreatment is achieved; compared with a traditional method, the method has the advantages that the detection reliability is greatly improved.

The present application relates to a kind of UPLC-MS / MS detection method for simultaneously determining 150 kinds of stimulant substances in cosmetics, belong to the technical field of stimulant detection.The detection method of the present application uses the pretreatment technology of solid phase extraction and QuECHERS, first, using the method of QuECHERS acetonitrile ultrasonic extraction, second, using the form of solid phase extraction to purify matrix, extraction tube selects filter type solid phase extraction column: Agilent Captiva EMR-Lipid filter column, unlike traditional HLB solid phase extraction column, the filter column does not need to activate and balance, can be self-eluted in the process of sample purification by gravity, and only need to add 20% pure water in the extraction liquid to achieve the purpose of activating filter column;While water can also be used as dispersing agent and impurity remover, the sample is dispersed while the impurities dissolved in water are also adsorbed.The detection method of the present application can meet the conditions of the pretreatment method of 150 kinds of stimulant substances in cosmetics under the condition of simultaneously extracting 150 kinds of stimulant substances in cosmetics, and realize the LC-MS / MS determination of 150 kinds of stimulant substances in cosmetics.

The invention discloses a method for simultaneously determining telolenol and telerate in plant-derived food based on liquid chromatography-tandem mass spectrometry, and belongs to the technical field of pesticide residue analysis. The method comprises the following steps: (1) extracting a sample by using a 1% acetic acid-acetonitrile solution; (2) a QuEChERS method with an optimized formula is adopted for purification, a purifying agent comprises anhydrous magnesium sulfate, a C18 adsorbent and graphitized carbon black, and PSA is not used; (3) carrying out liquid chromatography-tandem mass spectrometry analysis on the purified liquid, wherein a phenylhexyl column or a biphenyl column is adopted for chromatographic separation; and (4) quantifying by using a matrix matching standard curve according to the peak area. According to the method, the simultaneous accurate quantification of the telonol and the telonol ester is realized for the first time, the problem that mass spectrum behaviors of the telonol and the telonol ester are the same and are difficult to distinguish is solved, and the method is accurate, sensitive, efficient and suitable for detection of various plant-derived foods.