48 results about "Quechers" patented technology

The invention relates to a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) detection method for simultaneously determining 150 stimulant substances in cosmetics, and belongs to the technical field of stimulant detection. According to the detection method, a solid-phase extraction and QuECHERS combined pretreatment technology is adopted, firstly, an acetonitrile ultrasonic extraction mode in a QuECHERS method is adopted, secondly, a matrix is purified in a solid-phase extraction mode, an extraction tube adopts a filter type solid-phase extraction column, namely an Agilent Captiva EMR-Lipid filter column, different from a traditional HLB solid-phase extraction column, the filter column does not need to be activated and balanced, and the detection method is simple and convenient to operate. Gravity self-elution can be achieved in the sample purification process, and only 20% of pure water needs to be added into an extracting solution to achieve the purpose of activating a filtering column; and water can also be used as a dispersing agent and an impurity removing agent at the same time, so that impurities dissolved in water can be adsorbed while the sample is dispersed. The detection method provided by the invention can realize LC-MS / MS determination of 150 stimulant substances in cosmetics under the condition of simultaneously satisfying a pretreatment method for extracting 150 stimulant substances in cosmetics.

The invention discloses a multi-dimensional high-precision detection method and system for biotoxins in agricultural products, and the method comprises the steps: carrying out sterile collection and grading pretreatment on an agricultural product sample to obtain a homogenized sample; extracting the homogenized sample by adopting a mixed solvent system, adding an internal standard substance, carrying out salting-out layering, and collecting supernate; sequentially carrying out QuEChERS purification, multi-mechanism adsorption column purification and molecularly imprinted polymer specific enrichment on the supernate; carrying out dual-mode detection on the target toxin enrichment liquid, respectively carrying out quantitative detection by adopting a liquid chromatography-tandem mass spectrometry method, and carrying out screening detection by adopting an intelligent electrochemical sensing method; performing optimization processing on the dual-mode detection signal, and comprehensively judging a sample detection result; through integrated application of a triple purification mechanism, a dual-mode detection system, machine learning signal analysis and a full-chain quality control technology, efficient enrichment and deep interference removal of target toxins in a complex matrix can be realized, and both precision and applicability are considered.

The invention discloses an attapulgite-multi-walled carbon nanotube purification material as well as a preparation method and application thereof. The attapulgite-multi-walled carbon nanotube is used as a purifying agent in tea pesticide detection, matrix interferents can be effectively removed, the surface area of the attapulgite-multi-walled carbon nanotube is large, and pigments can be adsorbed from a complex matrix through the electron interaction and hydrophobic interaction of the multi-walled carbon nanotube; the porous structure of the attapulgite can physically adsorb and capture macromolecular impurities (such as proteins and polysaccharides), and amido bonds on the interface of the attapulgite-multi-walled carbon nanotubes can also adsorb polar molecules (such as organic acids). The attapulgite-multi-walled carbon nanotube interface is connected through a chemical bond, so that the phase separation problem of a physical mixed adsorbent is avoided, the material is uniformly dispersed in a solvent and can be in rapid contact with a matrix, and the purification efficiency is improved. Compared with a traditional QuEChERS method, the method has the advantages that a single material attapulgite-multiwalled carbon nanotube is adopted as a purifying agent, matrix impurities can be efficiently removed without multi-material combination, and the method is small in dosage, high in adsorption capacity, simple in experimental operation and low in raw material cost.

The invention discloses a sampling device for the content of a growth regulator in fruits and a synchronous sampling detection method, and aims to establish a rapid, simple, safe, reliable and high-specificity sampling detection method for multi-component PGRs in plant-derived food, improve the detection efficiency and reduce the detection cost, and by establishing a QuEChERS sample pretreatment method and combining an HPLC-MS detection technology, the content of a growth regulator in the fruits can be detected, and the content of the growth regulator in the fruits can be detected. According to the present invention, the combination of MgSO4, PSA, C18 and other purification materials is optimized, the PGRs residue rapid detection is performed in the fruit agricultural product matrix, the reference basis is provided for the related food quality safety supervision work such as multi-component PGRs residue detection, risk evaluation, standard formulation and the like, the sampling device can achieve the multi-point rapid equivalent sampling when the fruit is sampled, and the sampling efficiency is improved. Errors caused by manual sampling can be avoided, so that the detection result is more objective and accurate, and the operation is simple and convenient.

This invention discloses an integrated pretreatment tube and its working method for detecting mycotoxins in camellia milk. The pretreatment tube includes an outer tube with an outer tube cap at its top, a middle tube inside the outer tube with its top protruding from the outer tube cap and a middle tube cap at its top, and an inner tube inside the middle tube with its top protruding from the middle tube cap and its top cap. An ultrafiltration tube is located inside the inner tube, with its outer sidewall conforming to the inner sidewall of the inner tube, and its bottom forming an ultrafiltration element. At least one set of micropores is provided on the sidewall of the middle tube, and a first microporous filter membrane covers these micropores. At least one set of micropores is also provided on the sidewall of the inner tube, and a second microporous filter membrane covers these micropores. Through holes are provided on the sidewall of the ultrafiltration tube, and these through holes are directly opposite the positions of the second microporous filter membrane. A QuEChERS reagent pack is located at the bottom of the middle tube, and a salt pack is located at the bottom of the outer tube.

The invention discloses a QuEChERS-high performance liquid chromatography determination method for diarrheogenic illegal additives in food, and the method comprises the following steps: step 1, taking 2.0 g of a food sample, adding 10 mL of an extraction solvent, carrying out vortex oscillation-ultrasonic treatment, then adding anhydrous magnesium sulfate and sodium chloride, carrying out vortex mixing, carrying out centrifugal treatment, and extracting a supernatant; 2, taking 5mL of the supernate, adding the supernate into a purification tube containing purification salt, carrying out vortex oscillation-centrifugal treatment, taking the supernate, and enabling the supernate to pass through a 0.45 mu m polytetrafluoroethylene microfiltration membrane, so as to obtain a solution to be detected; and 3, injecting the solution to be detected into a high performance liquid chromatograph, carrying out gradient elution by adopting a C18 chromatographic column and taking acetonitrile-water as a mobile phase at a flow rate of 1.0 mL / min, a column temperature of 30 DEG C, a detection wavelength of 210 nm and a sample size of 10 [mu] L, and quantitatively analyzing a target compound through an external standard method. The invention aims to obtain a method for detecting illegal additives in food, which is low in cost and high in detection efficiency.

The invention discloses a non-targeted screening method for pesticide and veterinary drug residues in raw milk based on LC-TOFMS (liquid chromatography-time of flight mass spectrometry) and application of the non-targeted screening method, and belongs to the technical field of food safety detection. According to the method, a QuEChERS technology is adopted to carry out pretreatment on a raw milk sample, a liquid chromatography-time-of-flight mass spectrometry technology is utilized to carry out detection, and a self-built mass spectrometry database is combined to realize non-targeted qualitative screening. A self-established mass spectrum database comprises precise mass numbers, retention time, parent ion loading modes and secondary mass spectrum fragment information of 46 kinds of pesticides and 54 kinds of veterinary drugs. The method is simple in pretreatment, high in detection speed, wide in coverage range and suitable for rapid screening and quality monitoring of pesticide and veterinary drug residues in raw milk, and has good practicability and popularization value.

The invention belongs to the technical field of pesticide residue detection, and relates to a method for detecting residues of four herbicides in farmland soil through QuEChERS pretreatment-high performance liquid chromatography-tandem mass spectrometry. The method comprises the following specific steps: (1) drawing a standard curve: preparing a mixed standard solution of four pesticides including topramezone, bicyclopyrone, isoxaflutole and dicarfentrazone, carrying out high performance liquid chromatography-tandem mass spectrometry analysis, and drawing the standard curve according to the concentrations of the four pesticide standard solutions and quantitative ion pair peak areas; (2) QuEChERS pretreatment: performing solvent extraction, salt extraction and purifying agent treatment on to-be-detected soil to obtain a to-be-detected sample solution; and (3) analyzing the concentration of the pesticide residues: performing high performance liquid chromatography-tandem mass spectrometry analysis on the sample solution to be detected, and calculating to obtain the concentration of the four pesticide residues in the soil to be detected. The method is simple in pretreatment, high in detection recovery rate, good in detection result repeatability and high in detection sensitivity.

The application discloses a pesticide detection method for rice based on QuEChERS and ultraviolet-visible spectrophotometry, and takes three pesticides, i.e., bensulfuron-methyl, propanil and cypermethrin, as examples to carry out research on a rapid screening method for multiple pesticide residues in rice. The improved QuEChERS method is used to process rice to obtain a matrix liquid, matrix standard solution and solvent standard solution are prepared, the quantitative linear range of each pesticide and the matrix effect are obtained. In the quantitative concentration range, mixed solutions of the three pesticides in four combinations are prepared, the types of the pesticides in the rice are determined through support vector machine qualitative analysis, and the concentrations of the pesticides are predicted through partial least squares regression analysis, so that the pesticide residues in the rice can be effectively and rapidly detected.

The utility model discloses a QuECHERs sample purification column, and relates to the technical field of QuECHERs sample purification, the QuECHERs sample purification column comprises a main column body and a supporting assembly, the upper end of the main column body is connected with a sealing column cover, the supporting assembly is arranged outside the main column body, and the supporting assembly comprises a vertical sliding groove, a transverse sliding groove, a limiting sliding block, a mounting ring and a supporting column rod, transverse sliding grooves are formed in the two ends of the exterior of each vertical sliding groove, limiting sliding blocks are connected into the vertical sliding grooves, mounting rings are arranged outside the limiting sliding blocks, supporting column rods are additionally arranged on the two sides of the exterior of each mounting ring, and a purification assembly is arranged in the main column body and comprises a copper catalyst layer, a molecular sieve layer, an activated carbon layer and a microfiltration membrane layer. A molecular sieve layer is arranged below the copper catalyst layer, and an activated carbon layer is arranged below the molecular sieve layer. According to the QuECHERs sample purification column, the purification column can be stably and vertically placed on a table top, and the flexibility and motility of the purification column in the using process are improved.

The present invention discloses a method for detecting the content of phenidone in aquatic products, comprising the following steps: (1) dissolving phenidone in methanol to prepare standard solutions of different concentrations; (2) mixing the sample with acetonitrile, grinding and centrifuging to obtain a first supernatant; (3) placing the first supernatant in a flask containing PSA, C 18 and MgSO4 in a QuECherS purification tube, oscillated and centrifuged to obtain a second supernatant; (4) filtering the second supernatant through an organic filter membrane to obtain a sample solution; (5) respectively sending the standard solution and the sample solution to a high performance liquid chromatography tandem mass spectrometer for detection, and then comparing the obtained detection results to perform qualitative and quantitative detection. The present invention has simple pretreatment, accurate quantitative determination, and high sensitivity. The detection limit of phenidone is 5.0 μg / kg, and it can quickly and highly sensitively perform qualitative and quantitative detection of the phenidone content in aquatic products.

The invention discloses an FM550 in-vivo enrichment detection method and a risk monitoring method for tilapia, and relates to the technical field of ecological monitoring. The method not only can reflect the actual risk of a flame retardant in the environment, but also can be conveniently applied to aquaculture and ecological risk assessment. The detection method comprises the following steps: obtaining a liver sample and a muscle sample of a target tilapia mossambica, freeze-drying the liver sample and the muscle sample for 72 hours, and crushing and homogenizing 1 g of the liver sample and the muscle sample for later use; the target tilapia mossambica refers to tilapia mossambica which is exposed in an FM550 environment within 56 days; and carrying out solvent extraction, QuEChERS purification treatment and solvent conversion treatment on the crushed and homogenized sample, and then carrying out gas chromatography-mass spectrometry detection to obtain a detection result of FM550 in-vivo enrichment.

The invention provides a method for rapidly detecting bifenthrin in drug soil barrier soil based on a QuEChERS method in combination with ultra-high performance liquid chromatography. The method comprises the following steps: step 1, preparing a soil sample to be detected; step 2, extraction; step 3, purifying; step 4, detecting; 5, preparing a standard solution; 6, drawing a correction curve; step 7, combining calculation, adopting an external standard method for quantification, substituting the corresponding peak area into a correction curve formula for calculation, and obtaining the corresponding bifenthrin concentration; the method is easy and convenient to operate, the requirement for the operation proficiency degree is low, and operation can be completed without long-time training and high operation skills; a chlorine-containing solvent is not used, so that the environmental pollution is small, the problem that a great deal of time is consumed for cleaning experimental vessels for multiple times in an oscillation extraction method is solved in the aspects of experimental preparation, vessel cleaning and the like, and the working intensity is reduced; and the concentration of bifenthrin can be rapidly, simply and accurately detected.

The invention discloses a method for determining N-nitrosamine compounds in animal-derived food, and belongs to the field of food detection. The method comprises the following steps: freezing and grinding animal-derived food, mixing the animal-derived food with an internal standard solution, deionized water and acetonitrile, and freezing and preserving to obtain a pretreated sample; the method comprises the following steps: treating a pretreated sample through a QuEChERS salt bag, centrifuging, carrying out nitrogen blowing concentration, and mixing with deionized water to prepare an enriched solution; purifying the enriched solution by adopting a purification bag or specific functional resin, and centrifugally filtering to obtain a sample solution; and calculating the content of the target compound through a peak area ratio by using a liquid chromatograph-high resolution mass spectrometer provided with an APCI source and taking N-nitrosodimethylamine-D6 as an internal standard. The functional resin is formed by cross-linking specific functional monomers and is large in specific surface area and high in adsorption selectivity; the method has strong anti-interference capability, and can efficiently detect the N-nitrosamine compounds in dried aquatic products and other animal-derived foods.

The invention relates to a method for determining halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl, prothioconazole and metabolite residues of halauxifen-methyl in food. The method is simple, convenient and rapid to operate, good in precision and recovery rate, and suitable for confirmed quantitative determination of halauxifen-methyl, prothioconazole and metabolite thereof in food.

The utility model relates to an efficient purification device based on a QuEChERS method, and belongs to the technical field of sample pretreatment, the efficient purification device comprises a purification pipe filled with an adsorbent, the bottom of the purification pipe is provided with a microfiltration membrane, and the purification pipe is detachably provided with a piston rod. The method has the advantage of improving the sample purification efficiency.

The invention discloses a method for rapidly screening 25 environmental hormones in an aquatic product. The method comprises the following steps: performing QuEChERS extraction on the aquatic product to obtain a QuEChERS extracting solution; mixing the QuEChERS extracting solution with an extracting agent, water and an adsorbent to form a micro-emulsion system, centrifuging the micro-emulsion system, and enriching a target object in a solidified organic phase; carrying out gradient elution separation by adopting a C18 chromatographic column; and high-resolution mass spectrum data is acquired in a positive and negative ion switching mode. The method disclosed by the invention integrates efficient extraction, deep purification and high-sensitivity detection, and is suitable for efficient enrichment, purification and accurate detection of multi-component trace pollutants in a complex biological matrix.

The invention belongs to the technical field of analytical chemistry and food / traditional Chinese medicine safety detection, and particularly relates to a magnetic dispersive solid-phase extraction material and a preparation method and application thereof. The preparation method of the magnetic dispersion solid-phase extraction material comprises the following step: preparing the magnetic dispersion solid-phase extraction material Fe3O4 (at) MWCNT (at) Uio-67 from a Fe3O4 (at) MWCNT magnetic composite material by adopting an in-situ growth method. The material has ultrahigh specific surface area, rich adsorption sites and good magnetic separation characteristics, can be used in a QuEChERS pretreatment method, realizes rapid, sensitive and accurate detection of mycotoxin based on an ultra-high performance liquid chromatography-tandem mass spectrometry analysis method, and provides reliable technical support for quality safety control of related products.

The application discloses a kind of detection methods of nitrofurans metabolites in macrobrachium rosenbergii based on UPLC-MS / MS and application thereof, belong to aquatic product safety evaluation detection technical field.The application utilizes QuECHERS method to the pretreatment of macrobrachium rosenbergii sample, extracts nitrofurans metabolites, including adding acidified acetonitrile extraction, adding ammonium acetate, water removal and adding dispersed solid extraction column to reduce matrix effect and other steps.After the QuECHERS method of the application is handled, the obtained nitrofurans metabolites do not need to be subjected to derivatization procedure, can directly use HILIC hydrophilic chromatographic column, in positive ion mode liquid chromatography-mass spectrometry analysis, detect aminourea and other nitrofurans metabolites.The detection method provided by the application has the advantages of high sensitivity, strong accuracy, wide applicability, compared with traditional detection method, can avoid reagent waste, save a lot of time.

The invention discloses a method for detecting malachite green based on a fluorescence resonance energy transfer principle, a biosensor and a kit, and belongs to the technical field of aquatic product quality safety detection. According to the method, near-infrared light excited lanthanide doped up-conversion nanoparticles (UCNPs) are used as an energy donor, and a nucleic acid aptamer for specifically recognizing malachite green is covalently linked to the surface of the UCNPs to form the nano probe. When the malachite green does not exist in the sample, the aptamer is in a flexible conformation, and the fluorescence of the UCNPs is quenched due to the FRET effect. When malachite green exists in a sample, the aptamer is specifically combined with the malachite green and is subjected to conformational change, so that the spatial distance between the nanoprobe and a quenching agent is increased, the FRET process is blocked, and UCNPs fluorescence is recovered. The qualitative and quantitative analysis of malachite green can be realized by detecting the change of a fluorescence signal. The method has the advantages of high sensitivity, strong specificity, simple operation and rapid response, and is suitable for on-site rapid detection of malachite green residues in aquatic products and aquatic water.

The invention discloses a preparation method of a porous carbon nitride material and application of the porous carbon nitride material as a QuEChERS purification adsorbent, and belongs to the technical field of pesticide residue detection.The preparation method comprises the following steps that 1, a nitrogen-containing precursor and nano calcium carbonate are mixed and stirred in absolute ethyl alcohol and then transferred into a crucible, drying is conducted, and a solvent is volatilized; (2) calcining the obtained solid in a tubular furnace at high temperature to prepare a porous carbon nitride frame structure; (3) stirring the frame structure of the porous carbon nitride in hydrochloric acid, removing the calcium carbonate template, washing and drying to obtain a porous carbon nitride precursor; and (4) calcining the porous carbon nitride precursor in a tubular furnace at high temperature, washing and drying to prepare the porous carbon nitride material. When the porous carbon nitride material is used for detecting pesticide residues of agricultural products such as vegetables and fruits, operation is easy and convenient, the purification effect is better than that of classical purification materials such as PSA, C18 and GCB, and the detection accuracy can be effectively improved.

The invention relates to a method for determining five ultra-short chain perfluorinated compounds in agricultural products, which comprises the following steps: S1, weighing 1.00-5.00 g of homogeneous sample in a first centrifuge tube, adding a certain amount of internal standard and 1-5 mL of ultrapure water, and carrying out vortex mixing for 1 min; s2, continuing to add 10 mL of 2% formic acid acetonitrile, homogenizing at a high speed for 1 min, adding a QuEChERS pretreatment bag, performing rapid vortex mixing uniformly, and performing high-speed centrifugation for 5 min; s3, transferring the acetonitrile on the upper layer into a second centrifugal tube filled with a purifying agent, carrying out full vortex, and carrying out high-speed centrifugation for 5 minutes; s4, 5 mL of supernate is taken and put into a third centrifugal tube, nitrogen blowing is performed at the temperature of 45 DEG C until the supernate is dry, 1 mL of methanol water with the volume ratio being 1: 1 is added for constant volume, centrifugation is performed at the high speed of 12000 r / min for 10 min, and supernate is sucked and passes through a 0.22-micron filter membrane; and S5, detecting the supernate passing through the filtering membrane in the step S4 by liquid chromatography-tandem mass spectrometry. Due to the adoption of the technical scheme, the method for determining the five ultra-short-chain perfluorinated compounds in the agricultural product can be used for simultaneously determining the five ultra-short-chain perfluorinated compounds in the agricultural product.

This invention discloses the application of isohypericin in the preparation of a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*, thereby reducing the toxicity of pathogens without inhibiting their growth. This invention provides the application of isohypericin in the preparation of a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*. It offers a novel solution for developing highly efficient and safe food preservatives.

The invention discloses an electrochemical luminescence (ECL) sensor based on a luminous hydrogel and graphene oxide (GO)-aptamer interface and an acetamiprid detection method thereof. The luminescent hydrogel is formed by loading manganese dioxide nanosheets (MnO2NSs), N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and gold nanoparticles (AuNPs) grown in situ in a three-dimensional network of polyethyleneimine-glutaraldehyde (PEI-GA) together, and is used as an ECL luminescent layer with high density and stable microenvironment; a GO-aptamer composite layer is constructed on the surface of the GO-aptamer composite layer, and signal transduction of GO short-range quenching-target induced desorption-signal recovery (signal-on) is achieved. An ECL signal is collected in a buffer system containing a hydrogen peroxide co-reactant, and a quantitative relation is established by delta IECL or I / I0, so that sensitive detection of acetamiprid is realized; good linearity is realized in a range of 1.0 * 10 <-3 > to 1.0 * 10 < 3 > nM, and the detection limit is about 0.3 pM. The method is good in repeatability and stability, and can be combined with QuEChERS pretreatment to be used for sample detection of vegetables such as spinach, celery and leek.

The invention belongs to the technical field of detection of perfluoroalkyl and polyfluoroalkyl substances in traditional Chinese medicines, and particularly relates to an analysis method for determining perfluoroalkyl and polyfluoroalkyl substances (PFAS) in a traditional Chinese medicine pseudo-ginseng on the basis of ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry). According to the method, QuEChERS extraction is adopted, then through-type purification is performed through an enhanced matrix removal (EMR) small column, and finally LC / MS / MS detection is utilized. According to the analysis method provided by the invention, the sample pretreatment process is simple and efficient, the LC / MS / MS detection sensitivity is high, and reliable quantification is realized by using a matrix labeling curve. The analysis method provided by the invention is suitable for quantitative screening of 20 common PFAS in the plant-derived traditional Chinese medicine pseudo-ginseng.

The invention discloses a QuEChERS-gas chromatography-tandem mass spectrometry analysis method for simultaneously determining 12 N-nitrosamines in a meat product, and belongs to the technical field of chemical analysis and detection. The method comprises three steps of sample treatment, purification and detection, and efficient separation and accurate quantification of 12 N-nitrosamines, namely NDMA, NMEA, NDEA, NDPA, NDBA, NEPhA, NMPhA, NPIP, NPYR, NMOR, NDPhA and NDBzA, are realized by optimizing a QuEChERS pretreatment process, adopting a specific dSPE extraction bag and a purification tube and combining a DB-WAXETR chromatographic column and a dynamic multi-reaction monitoring mass spectrum mode. The method is simple and convenient to operate, rapid, high in sensitivity and good in accuracy, the detection limit is 0.25-0.60 mu g / kg, the adding standard recovery rate reaches 83.33%-111.83%, and the method is suitable for high-throughput detection and risk assessment of various N-nitrosamines in meat products.

The application discloses a kind of 3-nitropropionic acid residues in sugar cane and sugar cane juice Gas Chromatography-Tandem Mass Spectrometry detection method.The application uses acetyl chloride-methanol method, acetyl chloride and methanol are reacted to obtain hydrochloric acid-methanol, which makes 3-nitropropionic acid in sugar cane and sugar cane juice carry out methyl esterification derivatization reaction, and 3-nitropropionic acid methyl ester is generated.After extraction with toluene and centrifugation, the upper liquid is added to the QuEChERS extraction bag, vortexed for 1 min, centrifuged at 10000 rpm for 5 min, and then the upper liquid is filtered through a 0.22 μm filter membrane.The sample is detected by Gas Chromatography-Tandem Mass Spectrometry in multiple reaction monitoring mode, and quantified by external standard method.The method has high extraction efficiency and sensitivity, good accuracy and repeatability, and can be used to determine the residue amount of 3-nitropropionic acid in sugar cane and sugar cane juice.

The invention relates to the technical field of detection, and particularly discloses a high-throughput screening and quantifying method for anesthetics in aquatic products based on high-resolution mass spectrometry, which comprises the following steps: constructing a mass spectrometry database containing information of at least 72 anesthetic compounds; the method comprises the following steps: carrying out pretreatment on an aquatic product sample by adopting an improved QuEChERS method; carrying out analysis and acquisition under specific chromatography and mass spectrometry conditions by utilizing an ultra-high performance liquid chromatography-electrostatic field orbitrap high-resolution mass spectrometer; the method comprises the following steps: acquiring data by adopting a Full MS-ddMS2 scanning mode; and finally, analyzing the data through a'non-targeted discovery-targeted confirmation 'strategy, namely, firstly performing non-targeted screening by using Compressed Discovery software, and then performing targeted confirmation and quantification on the basis of the mass spectrum database through TraceFinder software, the method is high in flux, good in sensitivity and strong in anti-interference capability, and the method is suitable for large-scale popularization and application. Various types of anesthetic residues in aquatic products can be rapidly and accurately screened, identified and quantified, and the defects of an existing standard detection method are overcome.

The invention relates to the technical field of cosmetic detection, and discloses a liquorice plant-derived cosmetic quality evaluation method. According to the method, through four core dimensions of liquorice source authenticity evaluation, 37 kinds of pesticide residue detection, 37 kinds of element detection and 6 kinds of anti-infection illegal additive substance detection, an optimized pretreatment process and an accurate detection technology are combined, and comprehensive and efficient evaluation on the quality of the liquorice plant-derived cosmetics is achieved. According to authenticity evaluation of the liquorice source, nine characteristic components are detected by LC-MS, a QuEChERS combined GC-MS / MS method is adopted for pesticide residue detection, a super microwave digestion-ICP-MS method is adopted for element detection, and a Raman spectrum combined nanogold sol enhancement technology is adopted for illegal addition detection; and through four-dimensional weighted scoring (total score is 100), if the score is greater than or equal to 80, the product is qualified. According to the method, the defects that an existing detection method is insufficient in pertinence, large in interference and narrow in coverage range are overcome, and systematic and reliable technical support is provided for quality control, efficacy verification and safety guarantee of licorice plant-derived cosmetics.