512 results about "Mass spectrometric" patented technology

The invention discloses a micro proportional valve gradient composite coating and a preparation method thereof, and belongs to the technical field of micro proportional valve surface strengthening manufacturing. Aiming at the corrosion-resistant and wear-resistant requirements of the micro proportional valve under the working conditions of strong corrosion, multiple impurities and high pressure difference, the interface strengthening and corrosion-resistant and wear-resistant performance optimization of a metal matrix and a functional coating are combined, and metal / ceramic / polymer gradient material design, spraying parameter suitability research and a coating porosity / bonding strength control method are covered; the valve core and valve seat protection device is suitable for high-efficiency protection technical systems of valve cores and valve seats of precise instruments such as gas chromatographs and mass spectrometers.

A charge detection mass spectrometer includes an ion trap configured to store ions therein and to release stored ions therefrom, and an electrostatic linear ion trap (ELIT) array, in the form of at least two ELITs or ELIT regions each spaced apart from the ion trap, each ELIT or ELIT region including first and second ion mirrors and a charge detection cylinder positioned therebetween. The ion trap is controlled to release at least some of the stored ions to travel toward and into each of the ELITs or ELIT regions, and the first and second ion mirrors of each of the ELITs or ELIT regions is controlled in a manner which traps one or more ions traveling therein and causes the trapped ion(s) to oscillate back and forth between the first and second ion mirrors each time passing through and inducing a corresponding charge on the charge detection cylinder.

The invention provides an insulating oil gas mass spectrum analysis method and system. The method comprises the following steps: firstly, circulating vacuum degassing for one time, then deoiling and dehydrating the removed gas, introducing the deoiled and dehydrated gas into a mass spectrometry detection unit, carrying out primary detection, calling a self-adaptive algorithm and selecting proper circulating vacuum degassing times based on a primary detection result in combination with degassing temperature and degassing pressure of circulating vacuum degassing, and entering second-stage vacuum degassing; and deoiling and dehydrating the deoiled gas, and introducing the deoiled gas into a mass spectrometric detection unit for secondary detection to obtain a final detection result. According to the method, effective fusion of efficient degassing and rapid detection is realized, and reliable technical support is provided for rapid and accurate online monitoring and real-time fault diagnosis of the insulating oil dissolved gas.

The invention relates to the field of mass spectrometry, and describes a mass spectrometer based on multi-electrode coordination of a Jooden well. A multi-port ion source configured for multi-port input of electrons of ionization of gas molecules in a well and ions in an ion source outside the well, the ports being autonomously usable, comprising two external electrodes and four internal electrodes configured to excite periodic movement of ions in the well along an axis of symmetry and to detect signals caused by ions of secondary potential oscillation in the well, wherein ions are introduced into the trap from an external source with a slot, and ion movement is excited by applying an alternating voltage or the like to the electrodes. Or using a slot to introduce ions from an external source into a well in an external electrode, applying a potential to an internal electrode to create an electric field, the size of which is in a quadratic relationship with the coordinates, the electrodes having a specific equipotential surface geometry. Pulsed potentials can be applied to capture ions, multiple external ion sources can be equipped, and if the ion sources are not combined, each can be provided with a separate port. The mass spectrometer is uniquely designed in the aspects of ion input, excitation, detection, ion source configuration and the like.

The present invention relates to a method for quickly identifying white vaseline from different manufacturers based on silver salt-assisted laser desorption ionization-time of flight mass spectrometry, and belongs to the field of detection. Specifically comprising the following steps: S1, exploring and optimizing metal salt ion conditions, matrix conditions, and sampling methods, and using the optimized experimental conditions for sample preparation; S2, measuring the sample; S3, using eMSTAT Solution software for data analysis to achieve identification of white vaseline from different manufacturers. The method of the present invention replaces traditional organic matrices with silver salts, eliminates background interference, and improves the mass spectrometry signal-to-noise ratio; combines a direct smearing method to avoid complex solvent preparation and extraction, and is easy to operate; applies a mass spectrometer for mass spectrometry acquisition, has accurate molecular weight, reduces the detection time of each sample to 6 seconds, has high analytical throughput, and is low in cost, thereby achieving high-throughput, accurate, and rapid identification of large quantities of white vaseline from different sources.

Systems and methods described herein provide for charge state control of multiply-charged anions in a front end, high pressure ion guide. In some example aspects, a mass spectrometer system is provided comprising a first vacuum chamber (121) maintained at a pressure above about 500 mTorr. At least one ion guide (106) is disposed within the first vacuum chamber, the at least one ion guide comprising a plurality of rods extending along a central longitudinal axis. A controller (193) is configured to adjust an amplitude of an RF voltage signal provided to the plurality of rods so as to alternatively operate the ion guide in a first mode of operation with a lower amplitude of the RF voltage signal so as to reduce the likelihood of charge reduction by electron detachment so as to substantially maintain the isotopic distribution of the ions during transmission of ions through the ion guide, and a second mode of operation with a higher amplitude of the RF voltage signal so as to increase the likelihood of electron detachment from ions being transmitted therethrough.

Disclosed is a method and device for the mass spectrometric quantification of two or more known target ion species, in which a mass spectrometric signal, composed of signal components of the two or more target ion species, which are not mass resolved in the mass spectrum, is weighted with quantification functions and summed, and a quantification parameter is determined for the two or more target ion species from the weighted sums.

The invention discloses a traditional Chinese medicine decoction piece full life cycle data management system and method, and relates to the field of data processing, and the method comprises the steps: collecting traditional Chinese medicine planting environment data through a sensor, and encrypting and storing the data through a block chain; in the processing link, the moisture evaporation condition is predicted through an LSTM neural network, and an early warning mechanism is set; in the processing process, the process is adjusted through a Raman spectrum; components and pesticide residues of the finished product are ensured to be qualified through mass spectrometric detection; the clinical end collects prescription and curative effect data optimization process; all data are synchronized through a block chain and a cross-chain protocol; through a data lake architecture and a graph neural network model, a planting area and a processing technology are optimized. The system has the advantages that through technologies such as a sensor, a block chain and an AI model, whole-process data tracing and optimization from planting to clinical use are realized, the quality and curative effect of a traditional Chinese medicine product are improved, and transparency and safety of the whole process are ensured.

The invention discloses a method for detecting 18 phthalic acid ester substances in brewed sauce, which specifically comprises the following steps: step 1, obtaining a sample of the brewed sauce to be detected, adding a phthalic acid ester substance internal standard working solution containing deuterated isotope and water, uniformly mixing, adding sodium chloride and acetonitrile, continuously uniformly mixing, and detecting the content of the phthalic acid ester substances in the brewed sauce to be detected; carrying out ultrasonic extraction treatment for 5-15 minutes, adding an ultrasonic extracting solution into a mixture of PSA, C18 and MgSO4, and carrying out centrifugal treatment to obtain supernate; 2, carrying out nitrogen blowing on the supernate until the supernate is nearly dry, and fixing the volume by using normal hexane to obtain a sample to be detected; and step 3, detecting by adopting a gas chromatography-tandem mass spectrometer isotope internal standard method.

Methods and systems are disclosed for predictive classification of a raw mass spectrum of a sample, the spectrum resulting from mass spectrometry analysis. The predictive classification is performed by a trained large-scale spectral model, including a self-supervised large-scale base spectral model trained with unidentified sample spectra from a plurality of sources and fine-tuned with a task-specific model trained with identified spectra.

The invention discloses a method for determining the content of trace holmium, thulium and tungsten in rare earth erbium and a compound thereof, which comprises the following steps: placing a rare earth erbium and compound sample thereof in a beaker, and performing a blank test along with a sample; adding nitric acid, heating at low temperature until the sample is completely dissolved, and cooling; transferring into a volumetric flask, adding a cesium internal standard solution, diluting with water, and uniformly mixing; the holmium, the thulium and the tungsten are measured by using an oxygen mode of an inductively coupled plasma tandem mass spectrometer, and the contents of the holmium, the thulium and the tungsten are calculated according to a working curve method. According to the method, the inductively coupled plasma tandem mass spectrometry technology is used, and mass spectrum interference of an erbium matrix on holmium, thulium and tungsten is effectively eliminated by selecting a proper reaction mode and optimizing the flow of reaction gas.

The present invention relates to a mass spectrometric method for determining isomeric amino acid residues of a peptide and comprises the steps of: generating a list of peptide candidates using the amino acid lead sequence of the peptide, said lead sequence comprising positions of determined amino acid residues and at least one position with undetermined isomeric amino acid residues; predicting the collision cross section for each peptide candidate; comparing the predicted collision cross section of each peptide candidate with an experimentally determined collision cross section of the peptide and assigning the isomeric amino acid residues of the best matching peptide candidate to the isomeric amino acid residues of the peptide.

One mode of a chromatograph mass spectrometer according to the present invention includes: a measurement unit (1) that includes a chromatograph unit (1A) and a mass spectrometry unit (1B) capable of performing MS / MS analysis, and collects chromatograph mass spectrometry data having three dimensions of time, m / z, and a signal intensity by repeatedly performing the MS / MS analysis by data independent analysis in the mass spectrometry unit on a sample containing a compound separated by the chromatograph unit; a component detection unit (42) that detects a compound and a component corresponding to a partial structure of the compound by obtaining MS / MS spectra of a bar graph presentation based on chromatograph mass spectrometry data over a predetermined m / z range for a target sample, estimating precursor ion peaks in each of the MS / MS spectra, and selecting peaks based on a predetermined standard regarding an m / z direction in each of the MS / MS spectra and a predetermined standard regarding a time direction for peaks that can be considered to be identical or an identical group on the MS / MS spectra; a narrowing unit (43, 44) that narrows down components to be analyzed by performing screening using prior information on the detected component; and a composition estimation unit (45) that, by using m / z information corresponding to a narrowed down component, estimate a composition or a chemical formula of the component.

And re-sampling triggered by uncertain results in mass spectrum screening. The invention provides a system / technical scheme for promoting repeated sample injection triggered by uncertain results in mass spectrum screening. In various embodiments, the system may access a screening list specifying a plurality of target compounds. In aspects, the system can classify a plurality of target compounds as present or absent in a sample, respectively, based on causing a chromatograph and a mass spectrometer to perform an iterative injection scheme on the sample, with subsequent iterations of the iterative injection scheme having progressive compound selectivity, and is triggered by the uncertain presence or absence of the classification result in the previous iteration.

A system includes a first pre-separation device configured to perform a first pre-separation of precursor ions according to mobilities of the precursor ions and a second pre-separation device positioned downstream of the first pre-separation device configured to perform a second pre-separation of precursor ions based on a mass-to-charge ratio (m / z) of the precursor ions. The system further includes a mass spectrometer positioned downstream of the second pre-separation device configured to acquire mass spectra for precursor ions emitted from the second pre-separation device. The second pre-separation device is synchronized with the mass spectrometer such that an m / z range of precursor ions emitted from the second pre-separation device corresponds to a precursor m / z isolation window of the mass spectrometer.

The invention belongs to the technical field of medicine detection, and particularly relates to a method for detecting a nitroso compound in a raw material medicine in a riptacaine emulsifiable paste, which comprises the following steps: preparing a test sample: taking the riptacaine emulsifiable paste, adding a 5mol / L sodium hydroxide solution, uniformly mixing, then adding a 5mol / L hydrochloric acid solution, and uniformly mixing; diluting with a solvent with the pH value of 7.20 + / -0.02, and then centrifugally filtering to obtain a test sample; wherein the solvent is a mixed solvent composed of monopotassium phosphate and acetonitrile; liquid chromatography separation: the tested sample is separated by liquid chromatography, and a mobile phase A is a 0.2% formic acid solution; the mobile phase B is methanol; the chromatographic column is SB-C18, 2.1 * 100 mm, 1.7 [mu] m; and mass spectrum detection: detecting the substance separated by the liquid chromatography by using a mass spectrometer to obtain the content of the nitroso compound. The detection method is efficient, sensitive and excellent in reproducibility.

The invention provides an in-situ electrochemical nuclear magnetic resonance and online differential mass spectrometry combined method and device, and relates to the field of electrochemical detection. The method comprises the following steps: establishing a gas inlet passage and a gas outlet passage on an electrochemical nuclear magnetic resonance in-situ pool; an electrochemical reaction process in the electrochemical nuclear magnetic resonance in-situ pool is controlled through the electrochemical workstation, and electrochemical signals are collected; inert gas is introduced into an occurrence place of the electrochemical reaction process through the gas inlet channel, gas generated in the electrochemical reaction process is introduced into the mass spectrometer from the gas outlet channel along with the inert gas, and a mass spectrum signal is collected; nuclear magnetic resonance signals are collected through a nuclear magnetic resonance spectrometer. In the device, an electrochemical nuclear magnetic resonance in-situ pool assembly comprises a counter electrode binding post, a shell, a micrometer head and a working electrode binding post; an air inlet channel and an air outlet channel are formed in the micrometer head. According to the invention, an electrochemical signal, a nuclear magnetic resonance signal and a mass spectrum signal can be monitored at the same time, and real-time synchronous detection of solid, liquid and gas phases is realized.

The invention provides a chemical or enzymic method regenerated PET and PTA screening method, which comprises the following steps: pretreating a PET or PTA sample 1, and measuring the content of p-toluic acid, the content of p-carboxybenzaldehyde and the content of m-terephthalic acid by using high performance liquid chromatography; the method comprises the following steps: pretreating a PET or PTA sample 2, pretreating by using a high-temperature pyrolysis method, and measuring an isotope ratio of oxygen-18 to oxygen-16 by using an isotope ratio mass spectrometer; the method comprises the following steps: calculating a cleanliness index, an oxy-18 index and a bottle flake index by using a formula based on a test result, and classifying PET and PTA samples through a decision tree according to the indexes; combining and mixing the PET and PTA products based on the classification result and the requirements of downstream customers on the regeneration method and the regenerated material content; the method is convenient, accurate and reliable, the classification result is objective, the screening efficiency of PET and PTA regenerated by a chemical or enzyme method can be improved, and the economic cost is saved.

The present invention discloses a method for quantitative determination of trace harmful elements in various flotation products of rare polymetallic ores, which includes three parts: sample processing, preparation of complex matrix standard solution and sample testing. Each flotation product is digested with analytically pure concentrated nitric acid; the main mineral species of each product are determined by XRD, and the high-purity standard substances of each main associated mineral are digested with analytically pure concentrated nitric acid, and the content of harmful elements to be monitored is determined by ICP-MS after appropriate dilution; each standard substance digestion solution is diluted to a major element concentration of 1000μg / mL, and used to dilute the harmful element standard stock solution to obtain standard solutions with different concentration gradients; then the process blank and digestion sample are tested using an inductively coupled plasma mass spectrometer using a combination of internal standard method and matrix matching to obtain the content of trace harmful elements to be measured. It effectively overcomes the mass spectrometry and non-mass spectrometry interference problems faced by complex matrix sample testing, is suitable for the analysis and testing of trace element content in complex samples, and has a simple method and is easy to operate.

The invention belongs to the field of soil dissolved organic matter analysis, and provides a multispectral-based DOM detection and reclamation influence evaluation method, which comprises the following steps: step 1, collecting dry farmland soil samples of different reclamation years, and obtaining soil column samples of a plough layer, a plow pan and subsoil; 2, extracting, centrifuging and filtering the soil sample to obtain a DOM solution; 3, detecting the DOM solution by adopting an ultraviolet-visible absorption spectrum; step 4, adopting a three-dimensional fluorescence spectrum and combining parallel factor analysis to obtain fluorescence components and source characteristics of the DOM solution; step 5, analyzing molecular composition and characteristic parameters of the DOM solution by adopting solid-phase extraction in combination with Fourier transform ion cyclotron resonance mass spectrometry; and step 6, based on the detection data in the steps 3-5, evaluating the influence of reclamation years on the composition, structure and degradation characteristics of the DOM. According to the method, multi-dimensional detection of soil DOM is realized through a multi-spectrum and mass spectrum combined technology, and DOM is comprehensively analyzed from a macrostructure to molecular composition.

A method of adjusting a position of a tip of an electrode relative to an end of a nebulizer nozzle of a mass spectrometry device includes providing a conduit and the electrode connected to the conduit at a first end of the conduit. The electrode tip is disposed at a first position relative to the nebulizer nozzle end. The pressure gauge is connected to a second end of the conduit. A gas ejection is initiated from the nozzle with the electrode tip at the first position. During the gas ejection, the position of the electrode tip is adjusted from the first position towards a second position relative to the nozzle end. Adjusting the position from the first position towards the second position is terminated when the pressure gauge displays a pressure condition. Once adjusting is terminated, the electrode tip is at the second position.

The invention discloses a method for detecting perfluoroalkyl (polyfluoroalkyl) compounds in sludge, which comprises the following steps: screening concentrated sludge of a sewage plant, removing foreign matters, pretreating, putting into a centrifugal tube, and adding an extraction internal standard; the method comprises the following steps: extracting PFAS in sludge by using an alkaline organic solvent to obtain a PFAS extracting solution; filtering and acidifying the PFAS extracting solution to obtain an acidified extracting solution; purifying and eluting the acidified extracting solution through a solid-phase extraction column to obtain an eluent; drying the eluent, re-dissolving to 1mL by using methanol, filtering, and collecting the supernate into a sample injection bottle; and adding a sample introduction internal standard into the sample introduction bottle, mixing, and determining the PFAS in the sludge by using a liquid chromatography tandem mass spectrometer. By optimizing a pretreatment method for determining the PFAS in the sludge and a mode for reducing the matrix effect, a method capable of accurately quantifying the PFAS (51 different types) in complex environmental samples such as the sludge and biological solids is developed, and the analysis range and accuracy of the PFAS in the sludge are improved.

The invention relates to the technical field of detection, and discloses a detection method and application of p-phenylenediamine antioxidants and quinone derivatives thereof, and the method comprises the following steps: taking a blood sample or a tissue sample, placing in a centrifuge tube, adding a recovery rate indicator solution and a glutathione solution, adding an extraction solvent, extracting twice, and combining the two solutions; centrifuging for 4-6 minutes at the speed of 3000-4500 rpm after each extraction, and combining the two extracts to obtain an extract to be detected; concentrating the to-be-detected extracting solution to be dry under soft nitrogen to obtain a to-be-detected concentrated dry substance; purifying with / without an HLB column; further concentrating the eluent, adding acetonitrile for redissolving, passing through an organic filter membrane, putting into a small sample injection bottle, and concentrating again; adding an internal standard solution to a constant volume to obtain a to-be-detected sample solution; and finally, detecting the sample solution to be detected by using an ultra-high performance liquid chromatography system and a mass spectrometer. According to the invention, the blank of no corresponding detection method for various PPDs and PPD-Qs in human blood and tissues is filled up.

The invention belongs to the field of liquid chromatography-mass spectrometry detection, and discloses a liquid chromatography-mass spectrometry detection method for trace target objects in seawater, which comprises the following steps: firstly, configuring and precisely calibrating parameters such as flow velocity, column temperature, ion source voltage and the like for a liquid chromatography system, a chromatographic column, an automatic sampler and a tandem mass spectrometer; then, a methanol-water (containing 0.1% formic acid) system is adopted to implement linear gradient elution, polar difference component separation is considered, and analysis duration is compressed; a sample is subjected to solid-phase extraction, purification and concentration, then is automatically injected, is subjected to chromatographic separation and then enters an electrospray ion source; a mass spectrum end selects a dynamic multi-reaction monitoring (dMRM) mode, characteristic ion pairs are collected in a preset time window, and selective scanning and baseline noise suppression are achieved; and finally, utilizing retention time matching and internal standard correction to obtain a pg.L <-1 > magnitude quantitative result.

The invention discloses a 96-hole preassembled plate fat-soluble vitamin pretreatment kit, and belongs to the technical field of in-vitro diagnostic reagents. Comprising magnetic bead suspension liquid, equilibrium liquid, leacheate 1, leacheate 2 and eluent which are carried on a preassembled plate, and the magnetic bead suspension liquid comprises absolute ethyl alcohol and magnetic beads; the preparation process of the magnetic beads comprises the following steps: activating carboxyl of the carboxyl magnetic beads through EDC and NHS; then nitrochlorobenzene and triethylamine are added for a coupling reaction; mixing the coupling reaction product with pyrrolidone, ammonium persulfate and a pore-foaming agent to obtain target magnetic beads; the magnetic beads can be specifically combined with fat-soluble vitamins in human serum or plasma, efficient separation and release of an object to be detected are achieved through a magnetic separation technology, and the magnetic beads are suitable for follow-up mass spectrum quantitative analysis. The kit disclosed by the invention has the characteristics of simplicity and convenience in operation, high extraction efficiency and good stability, the pretreatment time can be remarkably shortened, the detection accuracy is improved, and an efficient solution is provided for clinical detection of fat-soluble vitamins.

The invention relates to the field of metal corrugated pipe leakage detection, and discloses a fixing device for metal corrugated pipe leakage detection and a helium mass spectrum leakage detection system, and the fixing device comprises a first device body and a second device body which are opposite to each other in a first direction and are arranged in a spaced manner to form an assembly space, a first communication hole is formed in at least one of the first device body and the second device body; the adjusting mechanism is used for adjusting the spacing distance between the first device body and the second device body in the first direction so as to change the size of the assembly space in the first direction. Therefore, through cooperation of the first device body, the second device body and the adjusting mechanism, the size of the assembling space in the first direction can be changed, metal corrugated pipes of different length sizes can be assembled in the assembling space, and therefore the universality of the fixing device can be improved, and the fixing device is simple in structure and convenient to use. And the assembly efficiency of the fixing device is improved, so that the leak detection efficiency of the metal corrugated pipe is improved.

The application discloses a chromatographic co-elution dimethyl phenol isomer high-resolution mass spectrometry characterization method and application, and the method comprises the following steps: according to the petroleum content range in a sample, selecting a corresponding sample pretreatment process to prepare a sample; under a methanol-5.0-6.5 mmol / L ammonia water mobile phase system, using LC-HRMS to detect a standard sequence sample, a conversion factor calibration sample and the sample, collecting primary mass spectrometry (MS 1 ) and selected ion secondary mass spectrometry (MS 2 ) data; according to MS 1 data, calculating the first normalized concentration of co-eluted 3,5-dimethyl phenol and 2,6-dimethyl phenol, and the first conversion factor of 2,6-dimethyl phenol to 3,5-dimethyl phenol; and the concentration of other 11 kinds of phenols and alkyl-substituted phenols; according to MS 2 data, calculating the second normalized concentration and the second conversion factor of the co-eluted components, and then calculating the concentration of 3,5-dimethyl phenol and 2,6-dimethyl phenol. The application realizes quantitative characterization of 13 kinds of C0-C2-alkyl-substituted phenol single components.

Before a sample is introduced into a liquid sample transport device, an ion source device receives an aqueous mobile phase solution from the liquid sample transport device and ionizes compounds in the aqueous mobile phase solution, thereby generating an ion beam. A tandem mass spectrometer performs a first neutral loss scan of the ion beam to generate a first intensity, wherein the first neutral loss value is set to the molecular weight of a first known solvent, and the tandem mass spectrometer performs a second neutral loss scan of the ion beam to generate a second intensity, wherein the second neutral loss value is set to the molecular weight of a second known solvent. A ratio of the first intensity to the second intensity is calculated. Based on the ratio, it is determined whether the aqueous mobile phase solution is being properly transported by the liquid sample transport device.

An apparatus for analyzing peak data generated from mass signals acquired by a mass spectrometric system comprises: a streaming device that comprises a first network interface and is configured to generate peak data from the mass signals or receive peak data generated externally from the mass signals, group the peak data into independently-processable data packets related to a processing task, and distribute the data packets in a task-specific stream via the first network interface onto a network; and at least one analyzing device that comprises a second network interface and is configured to retrieve a data packet of the task-specific stream via the second network interface from the network, perform the processing task on the retrieved data packet to produce result data, package the result data in a result data packet, and distribute the result data packet via the first network interface onto the network.

The disclosed methods are directed to preparing and detecting polypeptides using neutrophil elastase, such as human neutrophil elastase. The polypeptides are optionally denatured, reduced, and / or alkylated before being subjected to a first digestion. A second digestion comprises the use of neutrophil elastase, such as human neutrophil elastase. The prepared fragments are then analyzed chromatographically, electrophoretically, or spectrometrically, or a combination of these methods. The methods are especially useful for the preparation of therapeutic polypeptides for analysis, especially those that are not easily cleaved, such as some bi-specific T-cell engager (BiTE®) molecules.