1048 results about "Chromatographic separation" patented technology

The invention provides a method for determining hydrocarbon components in mixed aromatic hydrocarbons by using comprehensive two-dimensional gas chromatography-mass spectrometry. The method comprises the following steps: optimizing a target function to make a chromatographic peak signal obtained by a separation model accord with a chromatographic separation process mechanism, fully utilizing complementary information of chromatography and mass spectrometry, improving separation precision, and guiding analysis of complex aliasing peaks; analyzing overlapped chromatographic peaks determined by principal component analysis by adopting the established separation model to obtain a chromatographic peak signal representing a single component after separation, and matching the chromatographic peak signal with a corresponding mass spectrum; in combination with a single chromatographic peak signal and a mass spectrum obtained through separation, according to characteristic fragments and chromatographic retention time in the mass spectrum, component types corresponding to chromatographic peaks are judged, and characterization of the composition of the mixed aromatic hydrocarbon is achieved; a separated single chromatographic peak is subjected to quantitative analysis, the content of each component is calculated by adopting an external standard method in contrast with a standard curve, a quantitative result of the mixed aromatic hydrocarbon sample is obtained, and the measurement precision of the mass fraction of each hydrocarbon component is improved.

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.

The invention discloses a liquid chromatography-tandem mass spectrometry detection method and system, and the method comprises the steps: designing a multi-dimensional gradient elution program according to the physicochemical properties of a target compound, and obtaining a chromatographic separation spectrogram; performing efficient ionization on the target compound according to the chromatographic separation spectrogram to obtain an ionization signal; according to the ionization signal, fragmenting the target ions, and obtaining a fragment ion spectrogram through fragment ion screening; according to the fragment ion spectrogram, a multivariable data analysis algorithm based on machine learning is adopted, a standard curve of a target compound and an interferent database are combined, quantitative analysis is carried out, and a quantitative result is generated through a dynamic interference correction technology in combination with principal component analysis and a partial least square method. By utilizing the embodiment of the invention, the sensitivity, the accuracy and the real-time performance of detection can be improved through multi-dimensional gradient elution, dynamic ionization and fragmentation parameter optimization in combination with an analysis means of machine learning.

The invention relates to the technical field of liquid chromatography-mass spectrometry series connection, and particularly discloses a method for detecting folic acid metabolites of red blood cells through the liquid chromatography-mass spectrometry series connection technology. And an image analysis algorithm based on artificial intelligence and deep learning is utilized to extract a chromatographic separation state, so that gradient parameters are dynamically adjusted, the requirement of manual intervention is reduced, and the separation process of a target object in a complex matrix is favorably optimized. Particularly, key features in a chromatogram can be automatically identified and quantified based on a deep learning method, and gradient elution parameters are intelligently and dynamically adjusted according to the information, so that more optimized substance separation and detection can be realized. For example, if separation of a certain component is not ideal, the separation effect of the component can be improved by adjusting the proportion of the mobile phase B, so that the gradient elution process is optimized.

The invention discloses a gas component detection method and device based on circulation analysis, and relates to the related field of gas detection.The method comprises the steps that sampling is conducted when dynamically monitored real-time environment conditions meet set environment constraints; activating a chromatographic detector to perform chromatographic separation treatment on a target sample obtained by pretreating the gas sample; activating a mass spectrum detector to perform mass spectrum analysis on a first component in the multiple components to obtain a first mass spectrum; calling a mass spectrum identification plan to analyze the first mass spectrum to obtain a first detection result, and forming a target detection result; performing multi-dimensional property collection on the target sample based on a predetermined sample property to obtain a target property parameter; and correcting the target detection result by taking the combined detection fitness obtained by analyzing the target property parameters as a weight coefficient. The technical problem that existing gas component detection is insufficient in accuracy, comprehensiveness and reliability is solved, and the technical effect of improving the detection accuracy, comprehensiveness and reliability is achieved.

The invention provides a preparation method and application of boron isotope separation resin microspheres. The preparation method comprises the following steps: (1) adding dimethyl sulfoxide into polystyrene-divinylbenzene resin microspheres to swell for 3 hours, adding concentrated sulfuric acid to treat until the sulfonation rate is 0.3-0.4 meq / g, washing with water, treating with an ammonium chloride aqueous solution, washing with water, drying in vacuum, atomizing and spraying a ferric chloride-ferrous chloride aqueous solution, evaporating in vacuum to remove part of water, and introducing ammonia-containing nitrogen flow to treat to obtain polystyrene-divinylbenzene resin microspheres; and washing with water, grinding the surface, treating with a sodium hydroxide aqueous solution and washing with water. The boron isotope separation resin microspheres are suitable for chromatographic separation of 10B and 11B in a boric acid-sodium hydroxide aqueous solution with the pH of 6-7.5, a desorption solution is pure water, the feeding rate is 0.3-0.6 m / h, and a boric acid-containing aqueous solution enriched with 10B can be obtained; the service life of the chromatographic column is more than 6 months, the adsorption separation performance is reduced by not more than 20%, and the chromatographic column has a certain application prospect.

The invention provides ACE (Angiotensin Converting Enzyme) inhibitory peptide, middle-aged and elderly formula goat milk powder and a preparation method thereof, goat milk is taken as a raw material, the goat milk is sterilized and cooled, compound protease consisting of three proteases is inoculated for constant-temperature enzymolysis, and the ACE inhibitory peptide is obtained through ultrafiltration, preparative chromatographic separation and freeze drying. And uniformly mixing the active peptide with a nutrition enhancer and goat milk powder required by the middle-aged and elderly people to obtain the active peptide goat milk powder for the middle-aged and elderly people. Or after the goat milk is subjected to enzymolysis by compound protease, directly adding a nutrition enhancer required by the middle-aged and elderly people, and then concentrating and spray-drying to obtain the formula goat milk powder for the middle-aged and elderly people. The ACE inhibitory peptide prepared by the invention can be used as a food functional factor, and the prepared formula goat milk powder for middle-aged and elderly people contains the ACE inhibitory peptide and has the function of assisting in lowering blood pressure.

The invention discloses a method for detecting oxilafluorine and related components thereof, the method has high sensitivity and high accuracy, two impurity components existing in an oxilafluorine raw material and toothpaste are newly found, the chromatographic separation degrees of adjacent peaks are both greater than 1.5, and the oxilafluorine and the four impurity components are successfully analyzed and detected through HPLC-ELSD. According to the method, the content of the oxilafluorine and the related impurities can be calculated according to a peak area normalization method, or the content of the oxilafluorine and the related impurities in the toothpaste can be calculated according to an external standard method, the operation is simple, convenient and rapid, and the method is suitable for industrial production and quality control.

The invention discloses a method for preparing monodisperse silica core-shell microspheres by adopting a double template method, and relates to the technical field of chromatographic separation materials. The invention utilizes micron-level polymer microspheres and nanometer-level pore templates, and by adding silicon sources and catalysts at different time periods, the pore templates are coated on solid silica microspheres to form composite silica core-shell microspheres, and the microsphere templates and the pore templates are removed by dissolution extraction and high-temperature calcination to obtain silica core-shell microspheres with porous surfaces. The invention completes the control of the particle size of the microspheres, the thickness of the core-shell layer and the size of the pore structure in the same reaction system, and solves the problems of the traditional long time consumption and the occurrence of agglomeration. The silica core-shell microspheres prepared by the technology have good morphology and monodispersity, and the core-shell layer thickness and pore structure control method are simple, and are suitable for use in chromatographic separation.

A sample separation device includes a separation path and a control unit. The separation path is between a fluid drive and a sample separation unit, and is configured to inject a fluidic sample in an injector path into a mobile phase in the separation path driven by the fluid drive to the sample separation unit for chromatographically separating the fluidic sample. The control unit is configured to control blocking the injector path or at least a partial path thereof, generating an overpressure in the blocked injector path or the partial path thereof by a pressure source, and subsequently fluidically coupling the injector path or the partial path thereof to a region of lower pressure than the overpressure to generate an expansion stroke for releasing unwanted components from the injector path.

The invention discloses an enantiokaurane compound with antitumor activity as well as a preparation method and application of the enantiokaurane compound. According to the present invention, chemical components of Western African plant I.trichantha tubers are deeply studied, and a variety of chromatographic separation methods such as macroporous resin, silica gel resin, medium pressure preparation-MCI column, gel normal pressure column and semi-preparative high performance liquid chromatography are combined to separate to obtain one enantiokaurane new compound Trichanthone B; experiments show that the compound obtained through separation has a good inhibition effect on an MIA PaCa-2 pancreatic cancer cell line and an A549 lung cancer cell line, and IC50 of the compound on the MIA PaCa-2 pancreatic cancer cell line reaches a nanomole level; the effect of the compound is stronger than that of positive drugs 5-fluorouracil and carboplatin, and the compound has the potential of being developed into new drugs for resisting pancreatic cancer and lung cancer.

The invention relates to an air pre-impurity-removal control method and system for enrichment and separation of Kr, and relates to the technical field of gas separation and purification, and the air pre-impurity-removal control method comprises the following steps: removing impurity components in air through an impurity removal device to obtain air subjected to impurity removal treatment; carrying out multi-stage enrichment and separation treatment on the air subjected to impurity removal treatment to obtain enriched Kr gas; and introducing the enriched Kr gas into a chromatographic separation device, and separating krypton from other inert gases to obtain a target krypton component. The problems that in the traditional Kr enrichment and separation process, due to the fact that air impurities are prone to icing, the device is blocked, the multi-stage separation parameter adaptability is poor, and the enrichment efficiency is low are solved, and through pre-impurity removal of intelligent parameter configuration and multi-stage enrichment of gradient temperature control, the Kr separation stability, the enrichment efficiency and the target component purity are improved.

The invention discloses a polycyclic polyisopentenyl phloroglucinol compound as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The method comprises the following steps: by taking a dry overground part of hypericum monogynum as a raw material, performing percolation extraction and concentration to obtain an extract, suspending the extract in water to obtain a suspension, and performing extraction and concentration to obtain a petroleum ether part extract; the extract is taken and subjected to separation through a positive and negative phase silica gel column, MCI medium-pressure column chromatography and Sephadex LH-20 gel column chromatography and purification through high performance liquid chromatography, the polycyclic polyisopentenyl phloroglucinol compound is prepared, and the structure and configuration of the compound are determined by means of a spectrum analysis technology, quantum chemistry calculation, X-ray single crystal diffraction and the like. The compound can inhibit TNF-alpha protein to play an immunosuppressive role, and can be used as an immunosuppressive drug.

The utility model belongs to the technical field of sugar alcohol preparation, and relates to a system for co-producing L-arabinose and caramel pigment by using xylose chromatography raffinate, which comprises a raw material tank, a nanofiltration membrane separator, a chromatography separation device, an evaporation concentration tank, a crystallization tank, a centrifugal separator and a dryer which are sequentially communicated by pipelines, and the browning reaction assembly is used for mixing the raffinate and the centrifugal mother liquor and then carrying out browning reaction treatment to prepare caramel pigment. The chromatographic separation device is used for separating an extracting solution and raffinate from the flowing nanofiltration permeate, the evaporation concentration tank and the crystallizing tank are used for concentrating and crystallizing the flowing extracting solution into massecuite, the centrifugal separator is used for separating the flowing massecuite into moist sugar and centrifugal mother liquor, and the dryer is used for drying the flowing moist sugar into L-arabinose crystals. According to the utility model, raffinate and centrifugal mother liquor are mixed to prepare a caramel pigment liquid product, so that a caramel pigment additional product is obtained while the utilization rate of saccharide resources is improved.

The invention provides a traditional Chinese medicine extract component detection method and system, and relates to the field of traditional Chinese medicine detection. The component detection system for the traditional Chinese medicine extract comprises a sample preparation module used for pretreatment of dissolution and dilution of a sample and treating the traditional Chinese medicine extract sample into a solution state suitable for chromatographic analysis; the multi-dimensional chromatographic separation module comprises a first-dimensional chromatographic column, a second-dimensional chromatographic column, a pump and a sample injector, and is used for effectively separating different components in the complex traditional Chinese medicine extract sample so as to facilitate subsequent detection and analysis. The separation degree of a complex traditional Chinese medicine extract sample can be effectively improved by utilizing the selectivity difference of different chromatographic columns through a multi-dimensional chromatographic separation technology, meanwhile, the analysis time can be shortened and the analysis efficiency can be improved by optimizing the mobile phase composition and the gradient elution procedure, the sample loss can be reduced through multi-dimensional chromatographic separation, and the detection accuracy is improved. And the detection sensitivity and accuracy are improved.

The invention belongs to the technical field of chromatographic packing, and particularly relates to a double-layer hole type SiO2 (at) SiO2 core-shell microsphere based on a double-phase method and a preparation method and application of the double-layer hole type SiO2 (at) SiO2 core-shell microsphere. According to the core-shell microsphere, micron-sized non-porous SiO2 serves as a core, a mesoporous SiO2 framework with pore channels perpendicular to the surface of the non-porous SiO2 core serves as a porous shell layer, the porous shell layer sequentially comprises a first ordered mesoporous shell layer and a second ordered mesoporous shell layer which are different in pore diameter from inside to outside, and the pore channels of the two shell layers are completely consistent in direction and are communicated with each other. According to the invention, a porous shell structure with dual or multiple pore size distribution is constructed on the surface of a SiO2 core, and by integrating more than two pore sizes with obvious size difference, exclusive mass transfer paths are provided for solute molecules with different sizes, exclusive mass transfer channels can be provided for small-molecular compounds and macromolecular substances at the same time, and the pore size difference is greatly reduced. Therefore, the selectivity and column efficiency of chromatographic separation are remarkably improved.

The invention relates to the technical field of oilfield chemistry, and discloses a temperature-resistant and salt-resistant in-situ plugging reinforced gel composition, gel, and a preparation method and application thereof. The gel composition contains a first monomer, a second monomer, a first cross-linking agent, a second cross-linking agent, an initiator, a reinforcing agent and water, optionally, the composition also contains a third monomer. The gel provided by the invention can be re-crosslinked under the stimulation of high temperature and CO2 acidic conditions to form blocky integral gel, so that the problems of crosslinking uncertainty, chromatographic separation, easiness in causing reservoir damage and the like of an underground crosslinking system are avoided, and meanwhile, the enhancement of the plugging capacity under the in-situ reservoir condition is realized.

The invention belongs to the technical field of chromatographic packing, and particularly relates to a double-layer stacking hole type SiO2-coated SiO2 core-shell microsphere as well as a preparation method and application thereof. The core-shell microsphere takes micron-sized non-porous SiO2 as a core and takes a SiO2 nanoparticle stacking layer as a porous shell layer, the porous shell layer sequentially comprises a first SiO2 mesoporous shell layer and a second SiO2 mesoporous shell layer from inside to outside, SiO2 nanoparticles in the first SiO2 mesoporous shell layer are stacked to form a first gap pore channel, and SiO2 nanoparticles in the second SiO2 mesoporous shell layer are stacked to form a second gap pore channel. According to the invention, a porous shell structure with dual or multiple pore size distribution is constructed on the surface of a SiO2 core, and by integrating more than two pore sizes with obvious size difference, exclusive mass transfer paths are provided for solute molecules with different sizes, exclusive mass transfer channels can be provided for small-molecular compounds and macromolecular substances at the same time, and the pore size difference is greatly reduced. Therefore, the selectivity and column efficiency of chromatographic separation are remarkably improved.

The invention discloses an electro-synthesis method of alpha-aminomethyl tetrazole, which comprises the following steps: by taking a platinum sheet as a cathode, a carbon rod as an anode and tetrabutylammonium tetrafluoroborate as an electrolyte under the catalysis of constant current, coupling tertiary amine, isonitrile and azido trimethylsilane by a one-pot method to prepare an alpha-aminomethyl tetrazole derivative, and carrying out post-treatment on the obtained reaction mixed solution, and carrying out column chromatography separation to obtain a pure alpha-aminomethyl tetrazole product. The alpha-aminomethyl tetrazole can be obtained only through one-step reaction, the operation steps are few, a large number of oxidizing agents and additives are not used, the post-treatment process is simple, and the product is easy to separate.

The invention discloses a multi-alkyl aromatic alcohol extended block copolymer or oxysalt thereof as well as a synthesis method and application thereof. Molecules of the product contain polyalkyl, aryl, polyoxypropyl / ethyl and various ionic hydrophilic group structures, the product has excellent temperature resistance and salt tolerance and stable chemical properties, the adsorption loss in sandstone is extremely small, any alkaline agent does not need to be added, the crude oil recovery rate can be increased by 40% or above only through a single component at the concentration of 0.3 wt%, and the oil recovery rate can be increased by 30% or above. And the influence of performance degradation of the composite oil-displacing agent in an oil reservoir due to a chromatographic separation effect is avoided.

The invention discloses a novel lactitol production process which comprises the following steps: dissolving lactose, carrying out hydrogenation reaction on a dissolved solution, filtering a hydrogenated solution by a ceramic membrane, decolorizing filtrate particles by a carbon column, concentrating a feed liquid after ion exchange of a decolorized solution, carrying out chromatographic separation on a concentrated solution, adding a seed crystal into a chromatographic extracting solution, concentrating, melting and granulating a concentrated material, and drying a granulated product. And grinding the dried product, and screening the ground product to obtain a finished product. According to the method, the generation of fine crystals and pseudo crystals in the crystallization process of lactitol can be avoided, the constraint of a traditional crystallization process is avoided, and the environment is not influenced while the cost is saved.

The invention relates to the technical field of mass spectrum imaging, in particular to a non-targeted cascade mass spectrum imaging method and device, and the method comprises the steps: obtaining a second-level mass spectrum of each pixel point, and carrying out the spectrum peak recognition and peak extraction, so as to obtain a first intensity matrix, and performing first-order deconvolution calculation to obtain a plurality of parent ion secondary mass spectrums and a corresponding relation between the parent ion secondary mass spectrums and fragment ions, generating a second intensity matrix in combination with adjacent pixel molecule composition information, performing second-order deconvolution calculation to obtain relative ion intensity of characteristic fragments generated by each parent ion in each pixel, and generating a multi-target mass spectrum imaging result. Therefore, the problems that in related technologies, mass spectrum imaging cannot be combined with a chromatographic separation technology generally, so that the analysis dimension is limited, and the data analysis difficulty is increased; the problems that the complexity and the cost of an instrument are increased, the application scene is limited and the stability and the usability are influenced due to the fact that data do not depend on acquisition and spectrum diagram deconvolution depends on a molecular composition modulation technology are solved.

The invention discloses a preparation method of compound soybean oligopeptide with wide application, and belongs to the technical field of soybean processing. The preparation method comprises the following steps: mixing and stirring soybean powder and deionized water to obtain a mixed solution, performing first enzymolysis on the mixed solution, inactivating, adjusting the pH value, and performing centrifugal separation to obtain a soybean protein isolate precipitate and a supernatant containing water-soluble polysaccharide; oxidizing the water-soluble polysaccharide to obtain oxidized water-soluble polysaccharide; carrying out secondary enzymolysis on the soybean protein isolate precipitate, inactivating, and carrying out chromatographic separation to obtain a soybean oligopeptide mixed solution; the preparation method comprises the following steps: mixing and homogenizing oxidized water-soluble polysaccharide, a soybean oligopeptide mixed solution, a carboxymethyl chitosan solution, absolute ethyl alcohol and an emulsifier to obtain a mixed emulsion, drying and dewatering the mixed emulsion, then cross-linking to obtain a cross-linked solution, mixing the cross-linked solution with water to obtain a feed liquid, and carrying out spray drying on the feed liquid to obtain the composite soybean oligopeptide.

The invention relates to the technical field of lubricating oil analysis, in particular to a method and system for quantitative analysis of a lubricating oil additive. The method comprises the following steps: acquiring base oil data and lubricating oil additive data; performing mixed oil treatment based on the base oil data and the lubricating oil additive data to generate mixed lubricating oil additive data; performing mixed oil concentration detection on the mixed lubricating oil additive data to generate mixed oil concentration detection data; performing chromatographic column selection on the mixed lubricating oil additive data based on the mixed oil concentration detection data to generate chromatographic column selection data; and performing boiling point heating on the chromatographic column selection data to obtain mixed oil boiling point heating data. By optimizing data acquisition, chromatographic separation, component identification and quantitative analysis as well as result analysis and visualization, the accuracy of quantitative analysis of the lubricating oil additive is improved.

The invention discloses a bioactive peptide with antioxidant and hypoglycemic effects, a preparation and application thereof, and belongs to the technical field of bioactive peptides. The bioactive peptide provided by the invention is as shown in at least one of SEQ ID NO: 1 to SEQ ID NO: 4. Degreased shiny-leaved yellowhorn meal is used as a raw material, after high-temperature heat treatment and ultrahigh-pressure pretreatment are performed, the bioactive peptide with antioxidant and hypoglycemic effects is separated through enzymolysis, ultrafiltration and a liquid chromatography separation technology in combination with a bioinformatics technology, and components and sequences of the bioactive peptide are preliminarily identified through a liquid chromatography-mass spectrometry technology. And carrying out chemical synthesis according to the identified peptide fragment sequence to obtain four bioactive peptides with antioxidant and hypoglycemic effects. Through detection, the peptide fragments and the compound peptide fragments thereof have good alpha-glucosidase and alpha-amylase inhibitory activity, and have good application prospects in preparation of products with antioxidant and hypoglycemic effects.

The invention relates to a trace methane detection device and a detection method thereof, the trace methane detection device comprises: a sampling detection gas circuit formed by sequentially connecting a gas inlet, a three-way solenoid valve I, a quantitative tube, a sampling pump, a three-way solenoid valve II, a trace methane sensor and a gas outlet; the gas phase separation gas circuit comprises a constant flow pump and a chromatographic separation pipe which are connected between other ports of the three-way electromagnetic valve I and the three-way electromagnetic valve II, and the three-way electromagnetic valve I, the quantitative pipe, the three-way electromagnetic valve II, the constant flow pump and the chromatographic separation pipe form the gas phase separation gas circuit; and the signal processing and analyzing module is in signal connection with the trace methane sensor and is used for processing a detection signal of the trace methane sensor to obtain the methane concentration. According to the trace methane detection device and the detection method thereof, the to-be-detected gas sample is separated through the chromatographic separation tube, and the high-sensitivity gas sensor is used for detecting the gas concentration, so that the strong anti-interference performance brought by gas separation in a gas chromatographic analysis method is reserved, and the high-sensitivity detection capability of the gas sensor is combined.

The invention discloses an online pre-column derivatization coupled liquid chromatography-mass spectrometry amino acid detection method, and relates to the technical field of analysis and detection, and the method comprises the following steps: obtaining a to-be-detected sample, and carrying out protein hydrolysis treatment to obtain hydrolysate; mixing the hydrolysate with a derivatization reagent, carrying out on-line derivatization reaction through multiple sections of reaction zones which are connected in series, and maintaining different pH environments in the reaction zones; an online detection unit is arranged at an outlet of the reaction zone to monitor the concentration of derivative products and adjust reaction conditions in real time; performing chromatographic separation on the derivative reaction liquid; switching an ion source ionization polarity mode according to an elution time window to carry out mass spectrometry detection; and substituting the mass spectrum response signal into the standard curve to calculate the amino acid concentration value. The method realizes automatic derivation and high-sensitivity detection of amino acid, and has the advantages of simplicity and convenience in operation, controllable reaction conditions and high detection efficiency.

The invention provides an oligosaccharide debranching enzyme mutant and use thereof in a glucose mother liquor. The mutant is obtained by mutating valine at position 219 in SEQ ID NO: 1 into alanine. According to oligosaccharide debranching enzyme mutant V219A, a primary mother liquor, a secondary mother liquor, or a tail liquid after chromatographic separation is used as a substrate, the percentage contents of glucose in the products are 99.21% (primary mother liquor), 98.89% (secondary mother liquor) and 97.97% (tail liquid after chromatographic separation) respectively, which are 2.86%, 8.64%, and 28.67% higher than that of glucose obtained with the wild-type oligosaccharide debranching enzyme. Therefore, the mutant V219A obviously improves the percentage content of glucose in the glucose mother liquor, and the scope of application of the mother liquor can be expanded by the high product purity and substrate conversion rate, so the mutant V219A has higher industrial application value.

The invention discloses recombinant human XVII type collagen with a hemostasis function and application of the recombinant human XVII type collagen. The recombinant human XVII type collagen is a truncated protein of the human XVII type collagen, and the amino acid sequence of the recombinant human XVII type collagen is shown as SEQ ID NO: 1. The recombinant human XVII type collagen has efficient and rapid hemostatic performance, meanwhile, the recombinant collagen is easy to separate and purify, a pure product with the purity reaching 97% or above can be prepared through simple membrane separation and chromatographic separation, and the production process is easy to amplify. In the aspect of hemostasis performance, compared with commercially available products, the recombinant human XVII type collagen has the advantages that the hemostasis time is obviously shortened, and the hemostasis performance is obviously improved.

The invention belongs to the technical field of chromatographic separation materials, and discloses a preparation method and application of a GM-8 nanosheet material. The preparation method of the material comprises the following steps: respectively dissolving Cu (NO3) 2.3 H2O and 1, 3, 6, 8-tetra (4-carboxybenzene) pyrene in an organic solvent, mixing, transferring into a reaction bottle, adding trifluoroacetic acid and pyridine, and uniformly mixing and stirring to obtain a reaction mixture; and heating the reaction mixture to react for 7-9 hours to obtain a blue precipitate, cooling the blue precipitate to room temperature, and washing and drying the blue precipitate to obtain the ultra-stable microporous GM-8 nanosheet material. The nanosheet GM-8 is used as a gas chromatography stationary phase, high-efficiency separation and trace detection of xylene isomers can be realized, and the chromatographic column still keeps excellent separation capacity after being stored in an environment with air humidity of 65-90% for 31 months. The capillary gas chromatographic column can realize high-efficiency separation of disubstituted benzene isomers such as xylene, the separation degree of m-xylene / p-xylene is up to 18.2, and a new solution is provided for high-efficiency separation of isomers with similar properties.