380 results about "Chromatographic separation" patented technology

The invention discloses an online pre-column derivatization coupled liquid chromatography-mass spectrometry amino acid detection method, and relates to the technical field of analysis and detection, and the method comprises the following steps: obtaining a to-be-detected sample, and carrying out protein hydrolysis treatment to obtain hydrolysate; mixing the hydrolysate with a derivatization reagent, carrying out on-line derivatization reaction through multiple sections of reaction zones which are connected in series, and maintaining different pH environments in the reaction zones; an online detection unit is arranged at an outlet of the reaction zone to monitor the concentration of derivative products and adjust reaction conditions in real time; performing chromatographic separation on the derivative reaction liquid; switching an ion source ionization polarity mode according to an elution time window to carry out mass spectrometry detection; and substituting the mass spectrum response signal into the standard curve to calculate the amino acid concentration value. The method realizes automatic derivation and high-sensitivity detection of amino acid, and has the advantages of simplicity and convenience in operation, controllable reaction conditions and high detection efficiency.

The invention relates to the technical field of biomacromolecule separation and purification engineering, and discloses a chromatographic separation method for removing endotoxin in a vaccine preparation, which comprises the following steps: preparing an equilibrium buffer solution containing citrate with specific concentration and L-arginine hydrochloride; providing a mixed mode chromatography medium with hydrophobic and ion exchange complex ligands and balancing with a buffer; according to the method, through the synergistic effect of 8.0-12.0 mmol / L of citrate and 150-250 mmol / L of L-arginine hydrochloride, the endotoxin micelle structure is destroyed and is induced to be dissociated into monomers, and meanwhile, L-arginine is used for inhibiting monomer aggregation, so that the monomers are diffused into inner holes of the medium in a small size to be adsorbed, and the endotoxin micelle structure is separated into the medium. Therefore, the problem of separation caused by size overlapping of endotoxin micelles and antigens is solved, and double breakthrough of deep removal of endotoxin and high recovery rate of antigens is realized.

The invention discloses a stationary phase of a novel zwitterionic monomer containing a multi-charge structure as well as a preparation method and application of the stationary phase, and belongs to the technical field of hydrophilic interaction chromatography (HILIC) stationary phases. According to the method, multi-charge zwitterions are used as hydrophilic monomers, the multi-charge structure of the multi-charge zwitterions endows the monomers with higher hydrophilic performance, and the multi-charge zwitterions are immobilized on the surface of a matrix by utilizing an active free radical polymerization method, so that a series of novel super-hydrophilic HILIC stationary phases are finally prepared. The multi-charge zwitterionic monomer adopted by the invention not only has better hydrophilic performance and higher charge density, but also can efficiently, simply and conveniently realize matrix surface grafting and modification. The developed stationary phase is suitable for HILIC column chromatography separation analysis, and the HILIC chromatographic performance can be greatly improved; the method can also be applied to the fields of biological sample pretreatment, material science, marker clinical diagnosis and the like, and has high economic and social values.

The invention belongs to the technical field of radioactive metal isotope ion chromatographic separation, and discloses a medical radioactive isotope purification multichannel ion liquid chromatography system. The system comprises an automatic sample injection system, a parallel sampling and leaching channel, a parallel multi-column chromatographic structure, an online radiation dose monitoring module, a multi-channel tail end detection system, a recycling structure and a central control system. By adopting a full-automatic sample injection and multi-channel parallel chromatography structure, parallel separation, real-time radiation monitoring, multi-mode online detection and closed-loop recovery are realized, and the problems of insufficient nuclear purity, low separation efficiency, low target recovery rate and high radiation exposure of a traditional method are solved.

The invention discloses an active focusing system and method based on double-color off-axis illumination coupling small target surface high-speed detection, and belongs to the technical field of optical microscopic imaging. The invention aims to solve the problems that the existing active focusing technology cannot directly judge the defocusing direction, tentative movement is needed, and the speed is low. The system adopts a shared objective light-splitting dual-channel structure, simultaneous double-color off-axis illumination is utilized, and a local area of an objective view field is mapped to a high-speed image sensor through a zoom optical assembly in a detection channel; a control unit collects a single-frame image containing double-color information at a time, the displacement of pixels with symbols is directly calculated through chromatographic separation and multi-ROI weighted cross-correlation operation, and a pre-calibrated linear model is combined to drive a Z axis to achieve one-time accurate compensation. According to the invention, a tentative focusing step is eliminated, efficient decoupling of focus measurement and imaging is realized, and the focusing speed and robustness of high-throughput pathological section scanning are remarkably improved.

This invention discloses an online pre-column derivatization coupled with liquid chromatography-mass spectrometry (LC-MS) for amino acid detection, relating to the field of analytical detection technology. The method includes: obtaining a sample for protein hydrolysis to obtain a hydrolysate; mixing the hydrolysate with a derivatizing reagent and performing an online derivatization reaction through multiple reaction zones arranged in series, each zone maintaining a different pH environment; setting an online detection unit at the reaction zone outlet to monitor the concentration of the derivatized product and adjust the reaction conditions in real time; performing chromatographic separation of the derivatized reaction solution; switching the ionization polarity mode of the ion source according to the elution time window for mass spectrometry detection; and substituting the mass spectrometry response signal into a standard curve to calculate the amino acid concentration value. This invention achieves automated derivatization and high-sensitivity detection of amino acids, with advantages such as simple operation, controllable reaction conditions, and high detection efficiency.

The present disclosure relates to methods for quantifying both lipid and nucleic acid cargo components of LNP formulations simultaneously, i.e., in a single chromatography separation process. In some embodiments, the methods comprise ion-pair reversed-phase high performance liquid chromatography (IPRP-HPLC) separation coupled with ultraviolet (UV) and charged aerosol detection (CAD). In some cases, the methods allow for quantification of the content of several different lipid and nucleic acid species in a single LNP formulation simultaneously, thus allowing for efficient characterization of LNP formulations, for example, for process control of pharmaceutical or test formulations.

The invention relates to a method for rapidly preparing vernonia anthelmintica dealicin from vernonia anthelmintica dealicin, which comprises the following steps: carrying out ultrasonic extraction by using ethanol, enriching by using macroporous resin to obtain a vernonia anthelmintica dealicin extract, and carrying out extraction and pilot plant test preparative liquid chromatography separation to obtain a high-purity vernonia anthelmintica dealicin compound. The results of subsequent researches show that the vernonia anthelmintica dealicin can significantly inhibit the secretion of the chemotactic factor, and the inhibition rate of 1mol / L of vernonia anthelmintica dealicin on the release level of the chemotactic factor CXCL10 reaches 75.83%. The vernonia anthelmintica macrobrachin reduces the recruitment of self-reactive T cells to the skin by blocking a CXCL10-mediated inflammation signal channel, so that melanocytes are protected, and pigment regeneration is promoted. Therefore, the vernonia anthelmintica macrobrachin inhibits the secretion of the chemotactic factor CXCL10 in a targeted manner, can be used as a potential effective monomeric compound in the development of vitiligo medicines for research, and is expected to become a new strategy for treating vitiligo.

The utility model discloses a coke oven gas organic sulfur detection device which comprises a gas input system, a chromatographic separation column, a gas supply system, a detection system and a trace sulfur analyzer connected with the detection system, the gas supply system is used for supplying hydrogen, nitrogen and oxygen, the gas input system is used for conveying detected gas to the chromatographic separation column, the detection system comprises a flame photometric detector, and the flame photometric detector is connected with the chromatographic separation column. According to the device for detecting the organic sulfur in the coke oven gas, the chromatographic separation column is combined with the flame photometric detector, so that the content of the organic sulfur in the coke oven gas can be quickly and accurately detected.

This invention discloses a method and application for extracting soluble collagen from bones unearthed from archaeological sites, belonging to the field of archaeological sample processing technology. Specifically: 1) After pretreatment, the blocky ancient bone sample is dried at low temperature and ground into bone powder; the bone powder is added to an acidic solution for ultrasonic treatment, centrifuged, and the supernatant is filtered to obtain the sample to be loaded onto a column; 2) The sample to be loaded onto the column is subjected to chromatographic separation, using a gradient elution with equilibration buffer and elution buffer, and a UV detector is used to monitor protein peaks, collecting the target protein solution that elutes within 20-30 minutes; 3) Finally, the target protein solution is concentrated and purified using an ultrafiltration tube to obtain soluble collagen. This invention can obtain a sufficient amount of collagen from ancient deteriorated bone samples, significantly improving sample utilization and providing reliable technical support for bioarchaeological research.

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

The invention provides a system and method for detecting the content of a corrosion inhibition additive in cooling liquid, specifically, the system comprises a liquid chromatograph, a diode array detector DAD and an electric fog detector CAD which are sequentially connected in series, the liquid chromatograph comprises a mobile phase pump, an automatic sampler and a column oven, and a chromatographic column is arranged in the column oven; wherein the mobile phase pump is used for mixing different mobile phases according to a preset proportion and then conveying the mixed mobile phases to the automatic sample injector; the automatic sample injector is used for injecting the mobile phase from the mobile phase pump into the to-be-detected cooling liquid and flushing the to-be-detected cooling liquid injected with the mobile phase to the chromatographic column; the column oven is used for controlling the column temperature of the chromatographic column; the chromatographic column is used for performing chromatographic separation on the corrosion inhibition additive in the to-be-detected cooling liquid; the DAD is used for detecting the corrosion inhibition additive containing the benzene ring in the cooling liquid to be detected; the CAD is used for detecting the polybasic aliphatic carboxylic acid type corrosion inhibition additive in the cooling liquid to be detected.

The application discloses a desalination method for 1,3-propanediol fermentation liquor, and comprises the following steps: sequentially performing bacteria removal and concentration on the 1,3-propanediol fermentation liquor to obtain a primary concentrated solution; and performing specific chromatographic separation and desalination on the primary concentrated solution by using a chromatographic separation system to obtain a desalination solution; by the specific chromatographic separation and desalination process, the PDO fermentation liquor can be directly subjected to chromatographic separation and desalination after being filtered by ultrafiltration, the nanofiltration membrane filtration process is reduced, and the PDO extraction process is simplified; in addition, the product yield of PDO in the desalination process of the PDO fermentation liquor can reach more than 98%, the chromatographic separation and desalination rate is increased to more than 90%, the residual amount of the distillation kettle is significantly reduced, the product yield of PDO in the distillation process is increased, and the product quality is significantly improved.

The present invention relates to development of stationary phases and preparation of extraction columns having substantially improved capacity (i.e., amount of material purified per single chromatographic run) for lab scale to industrial scale extraction chromatographic separation, from small scale to industrial scale, of rare earth elements (REEs) and the platinum group metals (PGMs). More specifically the invention relates to preparation of stationary phases and extraction columns for extraction of REEs or PGMs as a group from containing matrices of typical REE or PGM feedstock and separation and purification of individual REEs or PGMs from each other.

The invention relates to a determination method and application of a Henan tobacco leaf sensory quality key group, and belongs to the field of preparation of tobacco leaf sensory quality key groups. The preparation method comprises the following steps: (1) carrying out gel column chromatography separation on Henan tobacco leaf extract, and segmenting fractions according to ultraviolet absorption conditions to obtain separated components; (2) carrying out independent evaluation and deletion recombination evaluation on each separated component; and (3) determining the sensory quality key group highlighting the sweet aroma style characteristic according to the evaluation result in the step (2). By means of the method, the group playing a positive role in the cigarette smoking quality can be obtained, and theoretical guidance is provided for tobacco leaf quality evaluation, tobacco flavor blending and the like.

The invention relates to the technical field of agriculture, and provides germacane type sesquiterpenoids in tithonia diversifolia as well as a preparation method and application thereof.The preparation method comprises the steps that stem and leaf parts of tithonia diversifolia are subjected to ethanol cold-soaking extraction, and an extracting solution is subjected to vacuum concentration to obtain a total extract; suspending the total extract with water, sequentially extracting with petroleum ether and ethyl acetate, and concentrating the extract liquor under reduced pressure to obtain each extraction layer; carrying out silica gel column chromatography separation on the ethyl acetate extraction layer, and combining to obtain six parts A-F; carrying out MCI column chromatography separation on the part D, and combining to obtain thirteen parts D1-D13; carrying out silica gel column chromatography separation on the D9 part, and combining to obtain fifteen parts D91-D915; and separating the D915 part by adopting HPLC (High Performance Liquid Chromatography) to prepare two germacane type sesquiterpenoids. The preparation method disclosed by the invention is simple and good in reproducibility, and the prepared germacane type sesquiterpenoids are high in purity and have specific herbicidal activity on foreign invasive plants such as ambrosia trifida and ambrosia esculenta.

This invention relates to the field of amino acid mother liquor resource utilization technology, specifically disclosing a method for the resource utilization of amino acid mother liquor. Specifically, the method involves: treating the amino acid mother liquor by electrodialysis to obtain an inorganic salt solution and desalted material; using the inorganic salt solution as an inorganic salt supplement for reuse in the amino acid fermentation production process; concentrating, crystallizing, and separating the desalted material to obtain crude amino acids and final mother liquor; and spray-granulating the final mother liquor to produce organic fertilizer. Compared with existing ion exchange and chromatographic separation technologies, the process provided by this invention has significant advantages such as simple flow, stable operation, no need for acid-base regeneration, and low equipment investment and operating costs. It achieves both amino acid resource recovery and the resource utilization of inorganic salt impurities, realizing a balance between clean production and economic benefits.

Chromatographic media comprising porous beads having an inner porous core and an outer porous shell are used for the chromatographic separation of enveloped or membrane-bearing biological particles from impurities such as contaminating DNA and / or proteins. The core is capable of binding molecules through hydrophobic interactions; however, the pore size of the shell prevents particles with a size of 20 nm or larger from penetrating into the beads and interacting with the core. Separation is performed at a pH below 7.4. The enveloped or membrane-bearing biological particles may have been subjected to a prior chromatographic capture step. When used for the purification of enveloped viral particles, the method of the present invention has been found to result in a significantly higher rate of infectious viral particles.

The invention relates to the technical field of pharmaceutical analysis equipment, and discloses pharmaceutical active component quantitative analysis equipment which comprises a chromatographic column, the chromatographic column comprises a hollow column body, a filter screen is fixedly arranged at the lower end in the column body, a stationary phase is arranged above the filter screen, a piston is arranged above the stationary phase, and the piston is connected with a driving part; the piston comprises a first shell facing the stationary phase, a second shell is coaxially arranged on the side, away from the stationary phase, of the first shell in a sliding mode, an elastic supporting piece is arranged between the first shell and the second shell, a plurality of connecting holes are coaxially formed in the bottom of the first shell and the bottom of the second shell, liquid discharging columns are arranged in the connecting holes, and liquid inlets are formed in the tops of the liquid discharging columns. A liquid outlet is formed in the side wall of the bottom of each liquid discharging column, the multiple liquid discharging columns are connected with a driving mechanism, and the second shell is connected with a liquid inlet pipe. According to the invention, the state of a stationary phase bed layer can be actively adjusted, and uniform introduction and dispersion of liquid medicine are realized, so that the chromatographic separation efficiency and the quantitative analysis accuracy are improved.

The invention discloses a glucose-fructose efficient separation and high-purity fructose preparation method, and belongs to the technical field of monosaccharide purification, the preparation method adopts functional resin as a stationary phase and water as an eluent, and chromatographic separation is carried out at 55-65 DEG C; the method specifically comprises the following steps: S1, filling a chromatographic column with functional resin, and balancing with 55-65 DEG C water; s2, pumping a mixed sugar solution of glucose and fructose into a chromatographic column; s3, eluting with water at 55-65 DEG C, and respectively collecting a glucose fraction and a fructose fraction; and S4, respectively concentrating, crystallizing and drying the glucose fraction and the fructose fraction to obtain a high-purity fructose product. According to the separation method, the purity of the obtained glucose is 94.4%-95.1%, the recovery rate of the glucose is 97.1%-98.0%, the purity of the obtained fructose is 98.6%-99.2%, and the recovery rate of the obtained fructose is 95.5%-96.8%.

The invention discloses a high-efficiency transfer hydrogenation method of non-activated olefin, which comprises the following steps: adding a non-activated alkenyl amide compound, nickel acetylacetonate dihydrate, phenylsilane, water and cesium pivalate into an organic solvent 1, 4-dioxane, reacting at room temperature under a nitrogen condition for 12 hours, and after the reaction is completed, filtering to obtain a filtrate, namely the high-efficiency transfer hydrogenation method of the non-activated olefin. And performing post-treatment (extraction and column chromatography separation) to obtain the corresponding alkyl chloride compound. According to the method, phenylsilane and water are directly used as hydrogen sources, direct use of hydrogen can be avoided, operation is easy and convenient, and efficient construction of C (sp3)-C (sp3) bonds can be achieved through transfer hydrogenation of non-activated olefin. The conversion reaction conditions are extremely mild, and the reaction activity is high; the method has the advantages of excellent atom economy and step economy, wide substrate application range, good functional group compatibility and the like. It is worthy that the synthesis method is also suitable for later-stage modification of medicine molecules, and the druggability of the molecules is expected to be further improved.

The present technology relates to commercial-scale methods for purifying botulinum toxin compositions obtained from cell cultures. Purification methods according to the present disclosure are based on a series of filtration and chromatographic separation steps that produce a high-purity botulinum toxin composition, which comprises botulinum toxin protein molecules (˜150 kDa) in solution, which is free, essentially free, or substantially free of botulinum toxin complexes and animal products, and without precipitating or lyophilizing botulinum toxin protein molecules. The purification method according to the present disclosure uses no precipitation, lyophilization, or centrifugation steps, permitting production of highly pure, highly active, free botulinum toxin protein molecules (˜150 kDa) in solution, without the need for reconstitution by the end user.

The invention relates to the technical field of biological medicine, and discloses a preparation method and application of a novel spironolactone derivative based on intestinal fungus biotransformation, and the method comprises the following steps: S1, screening an intestinal fungus Syncephalasetum monosporium as a transformation strain; s2, carrying out in-vitro fermentation culture on the screened strains; s3, extracting metabolites in the fermentation liquor; s4, identifying and separating the novel spirolactone derivative by combining mass spectrometry and a chromatographic separation technology; and S5, verifying the aldosterone receptor antagonistic activity of the derivative through molecular docking simulation. A novel spirolactone derivative is identified and separated through strain screening, fermentation condition optimization, multi-stage purification and mass spectrometry and chromatographic separation technologies, the enzyme activity of the strain is activated through shake culture in a culture medium to promote metabolism, the product purity is improved through inactivation, extraction and chromatographic purification, and the problems that a product is single and low in purity in a traditional method are solved.

The invention discloses a multi-mode liquid chromatography filler of ionic liquid hydrogel modified silica gel and preparation and application thereof, and the method comprises the following steps: fully dissolving an ionic liquid hydrogel monomer and a cross-linking agent in a polar aprotic organic solvent, adding silica gel, carrying out ultrasonic treatment, and standing; adding an initiator, stirring, condensing, refluxing, stirring and reacting at 70-80 DEG C in an inert atmosphere; and drying under stirring, centrifugally washing and drying. According to the invention, the problems of single functional group, tedious modification steps, low ligand density and poor high-pressure stability in the existing method are solved. The preparation method is simple and convenient, efficient modification of hydrogel on the surface of silica gel can be achieved, and the prepared liquid chromatography packing has good separation selectivity on nucleoside / basic groups, organic acid compounds, alkaloid, antibiotics, aniline compounds, anion-containing compounds and the like; and chromatographic separation in a hydrophilic chromatographic mode, a hydrophobic chromatographic mode and an ion exchange chromatographic mode can be realized.

The application provides a method for quantitatively detecting the concentration of dapsone in human plasma by using liquid chromatography-tandem mass spectrometry (LC-MS / MS). By optimizing the chromatography and mass spectrometry conditions, the detection specificity and sensitivity are improved. The protein precipitation pretreatment and rapid chromatography separation technology are adopted, the analysis time is shortened, the sampling amount is reduced, and the automation adaptability is good. The method can be used for efficient and accurate quantification, and is suitable for clinical pharmacokinetic research and treatment monitoring.

The invention discloses a construction method and application of a polysaccharide mass spectrum fingerprint spectrum. The method comprises the following steps: mixing a polysaccharide standard substance with a methylation reagent, directly introducing the mixture into an in-situ ionization source for direct ionization, and carrying out tandem mass spectrometry to obtain a mass spectrum fingerprint spectrum of the polysaccharide; a sample to be tested is subjected to the same testing process to obtain a mass spectrum fingerprint spectrum of the sample to be tested, the monosaccharide unit type of the polysaccharide is rapidly judged by analyzing the mass difference of characteristic continuous neutral loss in the spectrum, and accurate identification of the polysaccharide type is achieved by utilizing characteristic precursor ions and diagnostic fragment ions thereof. The method realizes direct and rapid analysis of complete polysaccharides, does not need tedious hydrolysis, derivatization and chromatographic separation steps, can effectively distinguish isomeric polysaccharides with different structures, and provides a novel high-throughput and high-discriminability analysis method for polysaccharide components in food, plant and biological samples.

The present application relates to the technical field of natural product extraction, and provides mulberry root bark extract and a preparation method thereof.The extract contains 3-8 characteristic components among eight characteristic components of mulberry flavone C, morusin, cyclo-morusin, cyclo-moracen, mulberroside G, morusin M, mulberroside C and mulberry flavone H.Further, the content relationship of four key components, the proportion of 300-800 Da components, the proportion of >1000 Da components, the content of fat-soluble resin impurities and the proportion of chromatographic peak area are coupled and limited by stability index S and synergy index T.The preparation method comprises the following steps: acid buffer infiltration, ethanol ultrasonic reflux extraction, concentration and adjustment of polarity, sedimentation separation, supercritical carbon dioxide impurity removal, resin column narrow window chromatographic separation, microfiltration-ultrafiltration-nanofiltration separation and drying.The obtained extract can regulate the expression of inflammation-related factors and promote the recovery of intracellular signal molecule level, thereby showing good soothing and skin barrier repair effects at the cell level.

A system for live compound visualization in column chromatography separation of compounds, including a shortwave UV transparent column configured to receive a crude mixture to be separated with an eluting solvent; a sorbent that is homogenously premixed with an inorganic UV indicator, wherein the fluorescent sorbent can be washed in-place and reusable; a shortwave UV light source that satisfies the following regarding its wavelength: 1) creates fluorescence on the inorganic UV indicator, 2) does not get absorbed by the eluting solvent, and 3) gets absorbed by the compounds; a detachable flow control valve rigidly fitted to an outlet end of the shortwave UV transparent column; a nozzle to the flow control valve configure to provide for eluent outflow of compounds separated from the crude mixture; and a hand-powered pressure bulb configured to provide low-pressure air flow into the shortwave UV transparent column attached to an inlet end of the quartz shortwave UV transparent column.

The application discloses a preparation method of an aggregation-induced emission fluorescent probe, and relates to the technical field of fluorescent probes, and comprises the following steps: synthesis of TPE-4NM, placing bis(4-(dimethylamino)phenyl) ketone and zinc powder in a double-neck flask, vacuumizing, filling argon, adding anhydrous tetrahydrofuran, slowly injecting titanium tetrachloride into the solution through a syringe, filtering, collecting the filtrate, concentrating under reduced pressure, separating through column chromatography, and obtaining TPE-4NM; synthesis of TPE-4NMB, dispersing TPE-4NM in anhydrous acetonitrile, then adding p-bromomethylphenylboronic acid, stirring and reacting overnight at room temperature, collecting the filter cake, redissolving after adding water, removing insoluble substances through centrifugation, freeze-drying the filtrate, and obtaining TPE-4NMB. ‑ The fluorescent probe can specifically detect and remove ONOO ‑ in a solution or cells, and after activation, exhibits typical aggregation-induced emission characteristics in an aqueous solution, has the advantages of strong water solubility, small toxicity, strong anti-interference ability and excellent selective removal of ONOO

The utility model discloses a column chromatography separation device which comprises a chromatographic column and further comprises a pressurizing structure which is detachably connected with the top end of the chromatographic column and is used for pushing a mobile phase in the chromatographic column to move downwards so as to accelerate the separation process, the switch is arranged at the upper part of the chromatographic column or the lower part of the pressurizing structure and is used for connecting or disconnecting the pressurizing structure and the inner cavity of the chromatographic column; the blocking piece is arranged in the chromatographic column, is positioned at the bottom of the stationary phase and is used for preventing the stationary phase from losing. The column chromatography separation device disclosed by the utility model is simple in structure, convenient to use, high in separation speed, good in separation effect and low in use cost.