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10 results about "Size-exclusion chromatography" patented technology
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Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are composed of dextran polymers (Sephadex), agarose (Sepharose), or polyacrylamide (Sephacryl or BioGel P). The pore sizes of these beads are used to estimate the dimensions of macromolecules. SEC is a widely used polymer characterization method because of its ability to provide good molar mass distribution (Mw) results for polymers.
A protein nanoparticle, a method for preparing the same, a pharmaceutical composition comprising the same, and a use thereof
The present disclosure relates to the field of medicine, in particular to a protein nanoparticle, a preparation method thereof, a pharmaceutical composition containing the same and uses. The protein nanoparticle is a nanoparticle component obtained by pretreating the supernatant of a stress-treated mesenchymal stem cell conditioned medium and then purifying by size exclusion chromatography, and the chromatogram obtained by size exclusion chromatography contains at least a peak with an apparent molecular weight of 56-70 kDa.
Owner:DARWIN BIOTECHNOLOGY (HUBEI) CO LTD
Integrated dual-mode chromatography to enrich extracellular vesicles from plasma
The present disclosure provides, in some embodiments, devices, methods, and kits for purifying extracellular vesicles (EVs) using size exclusion chromatography in tandem with cation exchange chromatography, which can be referred to as dual-mode chromatography (DMC).
Owner:THE GENERAL HOSPITAL CORP
A herbal exosome composition for eye care and treatment and a method of preparing the same
This invention relates to the fields of biomedicine and ophthalmic drug technology, specifically to an herbal exosome composition for eye care and treatment and its preparation method. The composition comprises a synergistic combination of herbal exosomes and mesenchymal stem cell exosomes, and also includes a nano-sustained-release carrier system. The herbal exosomes are extracted from the traditional herb licorice using gradient centrifugation combined with size exclusion chromatography, and the active herbal components astragaloside and quercetin are loaded onto the exosomes using electroporation to enhance their pharmacological activity. The nano-sustained-release carrier system has a biphasic sustained-release structure: the inner phase is a phospholipid complex carrying herbal exosomes and mesenchymal stem cell exosomes, and the outer phase is a thermosensitive hydrogel; it is liquid at room temperature, but rapidly transforms into a gel state after being instilled onto the ocular surface, significantly prolonging the drug's residence time on the ocular surface, improving bioavailability, and endowing it with targeted sustained-release capability against ocular surface tissues.
Owner:ORUIJUN (GUANGZHOU) BIOTECHNOLOGY CO LTD
A highly efficient continuous extraction system for vesicles from human umbilical cord mesenchymal stem cells
This invention relates to the fields of bioengineering and medical device technology, specifically to a highly efficient continuous extraction system for vesicles from human umbilical cord mesenchymal stem cells. The system includes an ultrasonic unit, a centrifugation unit, a size exclusion chromatography column purification unit, a collection unit, a closed-loop tubing system, and a control unit. This highly efficient continuous extraction system for human umbilical cord mesenchymal stem cells integrates three key technology modules—ultrasound, centrifugation, and chromatographic purification—and automates and closes the process, significantly reducing manual operation and sample exposure time, effectively lowering the risk of contamination, and improving extraction efficiency, purity, and activity of the vesicle products.
Owner:NANJING DRUM TOWER HOSPITAL
Characterization of cross-linking sites in antibody-drug conjugates
The present invention relates, in general, to a method for characterizing crosslinking sites of a target protein. In particular, the present invention relates to identifying and quantifying crosslinking sites of a target protein and determining the contribution of crosslinking to the formation of high molecular weight species using size exclusion chromatography, peptide mapping analysis, and subunit analysis.
Owner:REGENERON PHARMACEUTICALS INC
A method for detecting peptides using capillary gel electrophoresis
ActiveCN116698563BAdding Pathways to Analytical DetectionSolve the shortcomings of insufficient resolutionPreparing sample for investigationMaterial analysis by electric/magnetic meansGel electrophoresisSize-exclusion chromatography
This invention relates to a method for detecting peptides with a molecular weight below 10 kDa using capillary gel electrophoresis. The capillary gel electrophoresis of this invention can accurately characterize peptide impurities, especially aggregates, and serves as an effective supplement to size exclusion chromatography. It overcomes the insufficient resolution of size exclusion chromatography in analyzing peptide aggregates, while simultaneously detecting covalently bound aggregates. As a complementary technique to size exclusion chromatography, it provides multi-dimensional analysis of peptide aggregates.
Owner:ZHEJIANG UNIV +2
A method for quantitative analysis of intact proteins based on mass spectrometric tags
ActiveCN120294230BChemical labelingIntact protein
The application belongs to the field of chemical analysis, and particularly relates to a method for quantitatively analyzing intact proteins based on mass spectrometry tags. The specific technical scheme comprises the following steps: labeling a protein to be measured; separating the labeled protein by using a size exclusion chromatography-mass spectrometry combined technology; and quantitatively analyzing the protein to be measured by using a mass spectrometry mode of in-source fragmentation combined with multiple reaction monitoring. The application realizes the top-down high-sensitivity absolute quantification of intact proteins on a low-cost QqQ-MS by using a chemical labeling-in-source fragmentation-MRM combined strategy. The method for quantitatively analyzing proteins provided by the application has strong universality and good instrument compatibility.
Owner:CHENGDU INSTITUTE OF BIOLOGY CHINESE ACADEMY OF SCIENCES +1
Size exclusion chromatography method for polymer analysis in GHRH analogues
PendingCN122259753APerfect quality control systemensure safetyComponent separationPerfluoroacetic AcidChromatography column
The present application relates to a size exclusion chromatography method for polymer analysis in GHRH analogues, belonging to the technical field of biological medicine analysis. The present application develops a size exclusion chromatography method for polymer analysis in GHRH analogues, the sample to be measured is GHRH analogue, and an Agilent AdvanceBio SEC column is used; the mobile phase is a mixed solution of water, acetonitrile and trifluoroacetic acid, and isocratic elution is carried out; the method is especially suitable for tesamorelin or semorelin. The exclusive, efficient, accurate and stable size exclusion chromatography method developed by the present application is used for the analysis of polymer impurities in tesamorelin or semorelin, solves the technical problems of poor separation effect and insufficient detection sensitivity in the prior art, perfects the quality control system of GHRH analogues, comprehensively guarantees the safety and stability of the drug, and solves the technical problems urgently needed to be solved by the technical personnel in the field.
Owner:NANJING CHENGONG PHARM CO LTD
Preparation method and application of warm turmeric external capsule
The application discloses a preparation method of Wen-jujin-derived extracellular vesicles based on an affinity capture technology, in particular to a high-efficiency and mild preparation method using lectin affinity chromatography combined with tangential flow ultrafiltration and size exclusion chromatography, and belongs to the technical field of medicines. Embodiments of the application show that the Wen-jujin-derived extracellular vesicles have certain anti-ulcerative colitis activity in vivo and in vitro, and can be used for preparing medicines for treating ulcerative colitis.
Owner:WENZHOU MEDICAL UNIV
NK cell exosome capable of targeted killing of lung cancer and preparation method thereof
PendingCN122146600AMammal material medical ingredientsBlood/immune system cellsTumor targetingNatural Killer Cell Inhibitory Receptors
This invention discloses an NK cell exosome capable of targeting and killing lung cancer and its preparation method. Specifically, it is based on NK cells derived from umbilical cord blood stem cells, and involves constructing CRISPR-Cas9-PD-1 knockout recombinant plasmids to transfect cells, and rapidly inducing umbilical cord blood stem cells to transform into CD3+ cells using IL-21. ‑ CD56 + NK cell differentiation and maturation, tandem ultracentrifugation, and size exclusion chromatography were used for high-efficiency purification to obtain PD-1 knockout NK cell exosomes containing granzyme, perforin, and FasL. L NK-EXO). The PD-1 constructed in this invention L NK-EXO treatment significantly prolonged the survival of a mouse model of lung cancer and reduced tumor size, while also demonstrating clear lung cancer targeting and specific killing functions. More importantly, this invention reveals that PD-1 knockout of NK cell exosomes is crucial for tumor targeting and killing.
Owner:XIAMEN MEDICAL COLLEGE +1