11 results about "Mannuronic acid" patented technology

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The invention relates to the technical field of cosmetics, in particular to acer truncatum moisturizing pre-makeup cream and a preparation method thereof. The acer truncatum moisturizing makeup pre-cream is prepared from the following components in parts by weight: acer truncatum seed oil, cosmetic-grade purified water, cetyl PEG / PPG-10 / 1 polydimethylsiloxane, polydimethylsiloxane, isononyl isononanoate, synthetic wax, microcrystalline wax, glycerol, 1, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol the mannuronic acid tetrasaccharide micro-capsule is coated with 1, 3-propylene glycol / caprylyl glycol / ethylhexylglycerin, butanediol, sodium chloride, a sodium hyaluronate cross-linked polymer and temperature-sensitive polyglycerol-10 stearate. The acer truncatum seed oil containing 6% of nervonic acid serves as core lipid, temperature-sensitive type microencapsulated mannuronic acid tetrasaccharide and response type composite microcapsules are compounded, a'instant moisturizing-delayed moisturizing-barrier repairing 'triple synergistic system is constructed, the defects of a traditional technology are overcome, and the requirements of consumers for long-acting, efficient and mild pre-makeup care are met.

The invention belongs to the field of daily chemicals, and discloses an anti-wrinkle composition based on lipidosome delivery, which comprises the following components by weight: 0.1-1 part of a Chrysanthemum indicum extract; 0.1 to 1 part by weight of dipalmitoyl hydroxyproline; and 0.1 to 1 part by weight of methylsilanol mannuronate. According to the composition, dipalmitoyl hydroxyproline, a stellera chamaejasme extract and methylsilanol mannuronate serve as active ingredients, liposome serves as a carrier, and the anti-wrinkle effect can be achieved to the maximum extent. Meanwhile, the invention further discloses the lipidosome, application of the lipidosome and cosmetics.

The invention discloses a residual oil hydrotreating catalyst and a preparation method thereof, and belongs to the technical field of catalytic materials. The preparation method comprises the following steps: S1, mixing dolomite, cerous carbonate, a zirconium oxide precursor and a silicon dioxide precursor, drying and calcining to obtain a composite carrier, and performing in-situ pore forming by utilizing gas generated by decomposition of the dolomite and the cerous carbonate to form a mesoporous structure; s2, dipping the composite carrier in a modification liquid containing chitosan and mannuronic acid, and constructing a cross-linked network coating on the surface of the composite carrier through a Schiff base reaction to obtain a modified carrier; s3, dipping the modified carrier in a precursor solution containing a nickel source, a molybdenum source and a sulfur source, and performing adsorption, drying and roasting to obtain the catalyst. Through the synergistic effect of pore structure regulation and surface modification, the carrier with high porosity and suitable pore distribution is obtained, and meanwhile, high dispersion and stable loading of active metal components are realized. The catalyst shows excellent hydrodesulfurization, denitrification and demetallization performance in residual oil hydrotreatment.

The application provides a hybridoma cell strain secreting a monoclonal antibody against a alginate mannuronic acid tetrasaccharide epitope, a monoclonal antibody and application. In order to enhance the immunogenicity of the mannuronic acid tetrasaccharide, a glycoconjugate KLH-1 is used as an immunogen to inject and immunize a mouse, so that the mouse is stimulated to produce a specific immune response against the mannuronic acid tetrasaccharide epitope, then spleen cells of the immunized mouse are fused with myeloma cells, and after screening, an initial hybridoma cell strain is obtained; then after subcloning and specific screening, a hybridoma cell strain capable of stably secreting a monoclonal antibody of the target mannuronic acid tetrasaccharide epitope in alginate is obtained, and a monoclonal ascites antibody is further obtained. The monoclonal ascites antibody shows specific recognition and combination ability for pseudomonas aeruginosa, and the combination activity is related to the expression level of alginate on the surface of the bacteria. Therefore, the monoclonal ascites antibody can be used as a precise detection and diagnosis tool for pseudomonas aeruginosa, and can also be applied to the antibacterial treatment of pseudomonas aeruginosa infection.

A method for translocating a cationic micro / nanoparticle across a mucus layer, the method including (a) contacting the mucus layer with at least one alginate oligomer, the alginate oligomer having at least 70% mannuronate residues, together with the cationic micro / nanoparticle; or (b)(i) contacting the cationic micro / nanoparticle with an alginate oligomer having at least 70% mannuronate residues thereby forming a micro / nanoparticle carrying the alginate oligomer, and (b)(ii) contacting the mucus layer with the micro / nanoparticle prepared in step (b)(i). There is also provided a method for translocating a molecule of interest across a mucus layer including contacting the mucus layer with a micro / nanoparticle which is formed of self-assembling micro / nanoparticle forming components, wherein at least one is a cationic micro / nanoparticle forming agent and at least one is an alginate oligomer which has at least 70% mannuronate residues, and further comprises the molecule of interest, optionally wherein the molecule of interest is covalently bound to one or more of the self-assembling micro / nanoparticle forming components.

The application discloses a novel brown algae polysaccharide SARP for promoting healing of diabetic ulcers and a preparation and application thereof. A novel SARP is obtained through Sephacryl S-300 / HR gel column separation and purification, and the molecular weight of the SARP is about 45.4 kDa, which is composed of mannuronic acid (76.56%), guluronic acid (18.89%) and a small amount of glucuronic acid (4.55%). Compared with alginate separated from other brown algae, the SARP has a higher content of mannuronic acid. The SARP shows a better in-vitro antioxidant capacity, and can obviously reverse the cell damage of HUVECs caused by H2O2 and high sugar in the HUVECs. In addition, the SARP can promote the migration and tubular formation of the HUVECs. In-vivo experiments further show that the SARP can promote the healing of diabetic ulcer in rats, and can reduce the oxidative stress, inflammation level and lipid disorder in the rats. The SARP has a simple purification route, can be prepared in a large amount, and is a very promising novel drug for treating diabetic foot ulcers.

The invention described herein include the conversion of alginate that do not have the required mechanical and chemical properties for their use as alginate raft-forming oral suspension (AROS) for Gastroesophageal reflux disease (GERD) therapy into compliant alginate polysaccharides for AROS. The invention consists of the reaction of an epimerase enzyme with alginate polysaccharides containing a high amount of β-D-mannuronic acid sugars in their backbone to obtain alginate polysaccharide with high α-L-guluronic acid content. The reaction of the AlgE6R enzyme converts mannuronic sugars into guluronic sugars by creating long sequence of guluronic sugars increasing the blockiness of the resulting alginate. The invention combines the enzymatic treatment with a hydrolysis step to obtain alginate solutions that not only forms strong hydrogel but exhibit low viscosity in raft formulation offering the possibility to provide a better swallowability for GERD patients.

The application relates to the technical field of fertilizers, and particularly discloses a multifunctional suspending fertilizer, a preparation method, application and fertilization method thereof. The multifunctional suspending fertilizer comprises the following components: 10-20 parts of seaweed polysaccharide, 1-5 parts of seaweed oligosaccharide, 20-30 parts of a phosphorus component, 1-10 parts of a zinc component, 1-10 parts of a nutrient component, 30-40 parts of a suspending agent and 40-50 parts of water; the seaweed polysaccharide is obtained by enzymolysis of seaweed raw materials by a first enzyme preparation; the seaweed oligosaccharide is obtained by enzymolysis of seaweed polysaccharide by a second enzyme preparation; the first enzyme preparation is composed of cellulase, pectinase and glucose isomerase at a weight ratio of 1:(0.6-0.8):(0.4-0.6); and the second enzyme preparation is mannanase and / or guluronase. The multifunctional suspending fertilizer has the advantages of improving the content of vitamin C and sugar in fruits.

This invention discloses a catalyst for the hydrotreating of residual oil and its preparation method, belonging to the field of catalytic materials technology. The preparation method includes: S1. Mixing dolomite, cerium carbonate, zirconium oxide precursors with a silica precursor, followed by drying and calcination to obtain a composite support. In-situ pore-forming is achieved using the gas generated from the decomposition of dolomite and cerium carbonate, forming a mesoporous structure; S2. Impregnating the composite support in a modification solution containing chitosan and mannouronic acid, and constructing a cross-linked network coating on its surface via a Schiff base reaction to obtain a modified support; S3. Impregnating the modified support in a precursor solution containing nickel, molybdenum, and sulfur sources, followed by adsorption, drying, and calcination to obtain the catalyst. This invention achieves high dispersion and stable loading of active metal components while obtaining a high-porosity support with suitable pore distribution through the synergistic effect of pore structure regulation and surface modification. The catalyst exhibits excellent hydrodesulfurization, denitrification, and demetallization performance in the hydrotreating of residual oil.

This invention discloses the cloning and application of a mannuronic acid C5-isomerase gene related to high-temperature response in kelp, belonging to the field of biotechnology. This invention screens out a mannuronic acid C5-isomerase (MC5E) gene related to high-temperature stress response in kelp; based on this, specific primers are designed to clone the gene from kelp. MC5E The cDNA sequence of the gene was obtained; soluble recombinant protein was successfully expressed using a prokaryotic expression system, and high-purity MC5E protein was purified; 1H NMR spectroscopy analysis showed that this recombinant protein can convert mannuronic acid (M) in alginate to guluronic acid (G), increasing the G content in alginate; quantitative real-time PCR detection showed that under high temperature stress, kelp larvae... MC5E The significantly elevated transcription level of the gene indicates its involvement in kelp's response to high-temperature stress.