246 results about "Helicobacter" patented technology

The invention relates to the field of cubilose peptides, in particular to preparation and application of cubilose glycopeptides capable of preventing and assisting in treating gynecological inflammation and gastrointestinal inflammation. The invention provides cubilose glycopeptide which comprises cubilose peptide, sialylated O-glycan and sialylated N-glycan, and the cubilose peptide is connected with the sialylated O-glycan and / or the sialylated N-glycan. The cubilose glycopeptide provided by the invention has unique sialylated O-glycans and N-glycans, has unique biological activity and functions and synergistic effect compared with the cubilose glycopeptide without N-glycans or O-glycans and non-sialylated cubilose peptides, and forms and enhances the inhibition effect on pathogens and the antiviral effect; therefore, the compound has unique advantages in the aspect of preventing and treating related problems. The sialic acid binding type cubilose glycopeptide has stronger antibacterial activity, especially has an inhibition effect on pathogens such as candida albicans and helicobacter pylori, and also has an antiviral effect.

The invention discloses a compound microbial agent for reducing the cadmium content of brown rice and application of the compound microbial agent. Belongs to the technical field of agricultural microorganisms. The compound microbial agent is prepared from herbaceous spirillum, stenotrophomonas and coxakia, the herbaceous spirillum is herbaceous spirillum R3, and the preservation number of the herbaceous spirillum is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the stenotrophomonas is stenotrophomonas R5, and the preservation number of the stenotrophomonas is CCTCC (China Center For Type Culture Collection) NO: M 20231758; the coxakia is coxakia P1-4, and the preservation number of the coxakia is CCTCC (China Center For Type Culture Collection) NO: M 20231759. Compared with the prior art, the compound microbial agent disclosed by the invention has the beneficial effects that the compound microbial agent disclosed by the invention can reduce the content of cadmium in brown rice, so that farmland soil polluted by light and medium cadmium can be repaired and produced at the same time, and the edible safety of agricultural product rice is ensured.

The present invention belongs to the field of microbial technology, and specifically relates to a strain of Saccharomyces boulardii separated from fermented grains of Maotai-flavor liquor and its application. The strain is specifically Saccharomyces boulardii BD‑2, and its deposit number is CGMCC No.24930. Saccharomyces boulardii BD‑2 originates from the fermented grains in the fermentation process of the traditional fermented food Maotai-flavor liquor, and is a safe strain that can be used for food; it has strong acid resistance, bile salt resistance, high temperature resistance and gastrointestinal fluid resistance, as well as high self-aggregation, hydrophobicity, antioxidant activity and Helicobacter pylori inhibitory activity, has good in vivo survival ability and excellent prebiotic properties, and can be used in the fields of food, medicine, etc., such as winemaking, and the development of related functional foods and medicines.

The invention belongs to the technical field of probiotic preparations, and particularly relates to bifidobacterium longum subsp. Longum and application thereof in resisting helicobacter pylori. The bifidobacterium longum subsp. Longum provided by the invention has a good inhibition effect on helicobacter pylori. Furthermore, the bifidobacterium longum subsp. Longum is effectively embedded through enzyme cross-linking and ionic cross-linking by adopting the bifidobacterium longum subsp. Longum, so that not only can the embedding rate of the strain be improved, but also the survival rate of the strain can be remarkably improved in a digestion environment, and the efficient planting of the strain is realized; particularly, the effect of resisting helicobacter pylori infection can be obviously improved. Therefore, the invention has important practical significance on prevention and treatment of helicobacter pylori infection, and can also provide a technical basis for application of anti-helicobacter pylori functional products in the future.

The invention provides a lactobacillus plantarum KU01, and the preservation number of the lactobacillus plantarum KU01 is CGMCC (China General Microbiological Culture Collection Center) No.31788. The lactobacillus plantarum KU01 has the advantages that the preservation number is CGMCC No.31788; the invention also provides a fermentation product of the plant lactobacillus and a probiotic preparation comprising the plant lactobacillus and the fermentation product thereof. According to the probiotic agent and the preparation method thereof, the anti-helicobacter pylori probiotic agent is obtained through peracid stress fermentation, peracid stress fermentation, bacterial liquid separation, embedding, compounding and sieving. According to the method, on the basis of the inherent anti-helicobacter pylori physiological activity of the probiotics, the anti-helicobacter pylori physiological activity of the probiotics and the fermentation product is enhanced through stress fermentation, the fermentation product and the probiotics are sequentially released through an embedding technology, and an exogenous iron source is matched; and the anti-helicobacter pylori physiological activity is further promoted. The probiotics provided by the invention can be applied to resisting helicobacter pylori.

The invention relates to the technical field of microorganisms, in particular to lactobacillus rhamnosus HZBX-05 and application thereof.The lactobacillus rhamnosus HZBX-05 is sent to the China General Microbiological Culture Collection Center on July 27th, 2023 to be preserved in the China General Microbiological Culture Collection Center, the preservation number of the lactobacillus rhamnosus HZBX-05 is CGMCC NO.987, and the preservation number of the lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.28035, and the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing; the separated Lactobacillus rhamnosus HZBX-05 is continuously treated for 7 hours in a simulated gastric acid cholate environment, and the result shows that the HZBX-05 still has high survival rate, has the capability of being highly adsorbed to intestinal cells Caco-2 cells, has the capability of inhibiting various pathogenic bacteria, has immunoregulation and anti-inflammation effects, has the effect of resisting helicobacter pylori, and can be used for preparing an anti-inflammatory drug. The symptoms of female vaginitis can be improved.

The invention provides a probiotic composition for preventing and / or inhibiting oral pathogens as well as a derivative product, a preparation method and application of the probiotic composition, and belongs to the technical field of oral care. The probiotic composition comprises the following bacterial strains in parts by mass: 90 to 0.45 part of a lactobacillus rhamnosus bacterial strain A2114, 10 to 0.3 part of a plant lactobacillus bacterial strain A2124, 80 to 0.4 part of a pediococcus pentosaceus bacterial strain A2135, 250 to 0.25 part of a lactobacillus reuteri bacterial strain A21350, and 40 to 0.2 part of a plant lactobacillus bacterial strain A2109. The probiotic composition or the metabolite thereof can effectively inhibit oral pathogens such as streptococcus mutans, porphyromonas gingivalis, helicobacter pylori, escherichia coli and staphylococcus aureus. Therefore, the probiotic composition or the metabolite thereof shows a good application prospect in the aspects of preventing and relieving oral problems such as decayed teeth, periodontitis and ozostomia.

The invention relates to the field of microorganisms, in particular to lactobacillus plantarum TRT-01 with anti-inflammatory and bacteriostatic functions as well as a preparation method and application of the lactobacillus plantarum TRT-01, the lactobacillus plantarum TRT-01 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32237, and the preservation date is October 17, 2024. The plant lactobacillus has broad-spectrum bacteriostatic activity on 10 pathogenic bacteria such as helicobacter pylori, streptococcus mutans and the like, and can regulate Th1 / Treg immune balance and relieve inflammatory response by inducing dendritic cells to secrete cell factors IL-10 and IL-12, inducing T cells to secrete cell factors TGF-beta and IFN-gamma and inhibiting secretion of cell factors IL-6 at the same time. Therefore, the plant lactobacillus TRT-01 disclosed by the invention can be used for preparing a composition with anti-inflammatory and bacteriostatic effects, is suitable for the fields of medicines, foods, health-care products and the like, and is particularly suitable for developing a microbial preparation with a probiotic effect.

The invention relates to the technical field of pathogenic bacterium detection, in particular to a method and a kit for detecting helicobacter pylori gene mutation in excrement. According to the technical scheme, the kit for detecting helicobacter pylori gene mutation in excrement is developed by combining multiple advanced technical means such as ARMS-PCR design, blocking primers, locked nucleic acid modification (peptide nucleic acid modification), MGB probes, nested PCR design and degenerate base design. The kit can detect nine gene mutation sites related to the drug resistance of helicobacter pylori at one time, and has a wide coverage range. The kit in the scheme can be used for detecting the drug resistance of quinolone antibiotics and / or clarithromycin antibiotics of helicobacter pylori, and the technical problem that a high-specificity and high-sensitivity excrement helicobacter pylori gene mutation detection method is lacked in the prior art is solved. The method is simple and convenient to operate and can be completed within 1-2 hours, the detection cost is reduced, and the method has ideal application and popularization prospects.

The present application provides a wood frog antimicrobial peptide preparation for Helicobacter pylori and a preparation method thereof, wherein the wood frog antimicrobial peptide preparation comprises a polysaccharide-modified wood frog antimicrobial peptide concentrate. The wood frog antimicrobial peptide preparation of the present application can maintain the antibacterial effect and antioxidant activity for a long time in a low-pH gastric fluid environment, and is expected to be used as an oral drug to kill Helicobacter pylori in the gastric environment, providing a new class of drugs for the treatment of Helicobacter pylori infection.

The invention relates to the technical field of medicines, and discloses a medicine for treating helicobacter pylori infection, which comprises the following raw materials: a substance a and pharmaceutically acceptable auxiliary materials, the structural formula of the substance a is shown as a formula (I). The substance a has a good bactericidal effect on helicobacter pylori and can be used for treating and preventing helicobacter pylori infection; and the substance a has a specific structure, can realize efficient sterilization in vivo and can be rapidly degraded at the same time, the degradation product is rapidly discharged out of the body, the substance a and the common degradation product thereof are actually nontoxic and very high in safety, and the substance a cannot be absorbed by the human body, has no side effect and cannot cause the helicobacter pylori to generate drug resistance.

The invention discloses a hydrogel-based helicobacter pylori killing medicine and a preparation method thereof in the technical field of biomedical materials, and the hydrogel medicine adopts a shell-core layered structure and comprises an outer layer and an inner layer; the outer layer is a high-density cross-linked polyethylene glycol diacrylate network, and cellulose nanocrystals modified by silane are dispersed in the polyethylene glycol diacrylate network; pH-sensitive Schiff base bonds and / or boric acid ester bonds are embedded in the inner layer, and an antibacterial agent is loaded on the inner layer. Structural expansion and breaking of the hydrogel are controlled through a multi-layer structure, multiple loaded antibacterial agents are released according to pH response, the influence of drug resistance is avoided through sterilization of multiple mechanisms, the preparation process and device of the hydrogel drug are integrated, the production cost is reduced, and the clinical conversion efficiency is guaranteed.

The invention discloses a high-adhesion plant lactobacillus DACN962 strain and an application of the high-adhesion plant lactobacillus DACN962 strain in inhibition of helicobacter pylori. The plant lactobacillus DACN962 provided by the invention has a remarkable effect in prevention of helicobacter pylori colonization, and the DACN962 can compete for inhibiting the helicobacter pylori from being adhered to GES-1 cells in vitro and can be up to 53.55 + / -1.54%; in-vitro exclusion inhibition of helicobacter pylori adhesion to GES-1 cells can reach up to 82.98 + / -2.37%; the diameter of the inhibition zone of the freeze-dried supernatant of the plant lactobacillus DACN962 can reach 22.69 + / -0.76 mm, and the diameter of the inhibition zone of the fermentation liquor (containing thalli) can reach 15.33 + / -0.82 mm. The DACN962 is good in safety, has high adhesiveness, can compete / exclude and inhibit adhesion of helicobacter pylori, has helicobacter pylori resistance, is sensitive to various antibiotics, and is safe and harmless to a human body.

The invention provides a method for preparing carbon isotope labeled urea by taking divalent carbonate as a raw material, and belongs to the technical field of helicobacter pylori detection. The method comprises the following steps: S1, mixing acid with divalent carbonate powder, and collecting generated carbon dioxide; s2, drying the carbon dioxide, and introducing a nitrogen source solution until the carbon dioxide is saturated to obtain a reaction solution; s3, sequentially carrying out heating reflux, cooling, filtering, solvent removal and vacuum concentration on the reaction liquid to obtain carbon-labeled C (NTMS) 2; s4, dissolving the carbon-labeled C (NTMS) 2 in 1, 4-dioxane, then adding a hydrogen fluoride aqueous solution and oxalic acid, carrying out a reflux reaction, collecting a solid, and drying to obtain carbon isotope labeled urea; the divalent carbonate in the S1 is marked by carbon 13 or carbon 14. The high-purity carbon isotope labeled urea is prepared by adopting the carbon labeled divalent carbonate as the raw material, the preparation condition is mild, the synthesis process is convenient, green and environment-friendly, and the method is suitable for industrial production.

The invention relates to the technical field of food microorganisms, in particular to lactobacillus plantarum Lrld-22 and application thereof. According to the invention, the strain Lrld-22 is separated from Qinghai yak fermented milk, the strain Lrld-22 is used for fermenting hickory nut and torreya grandis fermented milk for domestication, and the new lactobacillus plantarum is separated and identified from the hickory nut and torreya grandis fermented milk. Research tests show that the lactobacillus plantarum has the functions of reducing uric acid, relieving gout symptoms and removing helicobacter pylori at the same time, and a good basis is provided for enriching the application of the types of probiotics.

The invention belongs to the technical field of synthesis, and particularly relates to a synthesis method of urea [14C]. The synthesis method of the urea [14C] comprises the following steps: (1) reacting carbonate [14C] with ammonia gas to obtain cyanamide salt [14C]; (2) hydrolyzing the cyanamide salt [14C] obtained in the step (1) with concentrated sulfuric acid at the hydrolysis temperature of 35-45 DEG C; and (3) adjusting the pH value of the hydrolysate obtained in the step (2) with ammonia water, concentrating and purifying to obtain urea [14C]. The synthesis method provided by the invention has the advantages of mild conditions, efficient reaction, high product yield (up to 95% or above), high purity (the chemical purity is greater than or equal to 99%) and the like, and is suitable for large-scale preparation of key raw materials of helicobacter pylori diagnostic reagents.

The embodiment of the invention discloses a primer group and application. Specific primers are designed for a UreA gene, a GyrA gene, a 23SrRNA gene, a PbP1 gene, a 16SrRNA gene, a porD gene, a CagA gene, a vacA gene, a CYP2C19 * 2 gene, a CYP2C19 * 3 gene, a CYP2C19 * 17 gene and mutation sites on the basis of a nucleic acid mass spectrometry technology, the helicobacter pylori detection kit has the advantages of high sensitivity and high accuracy, and helicobacter pylori identification, antibiotic sensitivity / drug resistance detection and virulence gene typing can be quickly and efficiently carried out; the method for detecting the metabolic type of the proton pump inhibitor has a good application prospect.

The invention provides three pairs of helicobacter pylori antibodies and application thereof, and limits amino acid sequences of heavy chain complementary determining regions and light chain complementary determining regions of the antibodies. The helicobacter pylori antibody provided by the invention has the advantages of good specificity, high biological activity, strong stability and high affinity with helicobacter pylori, and can be used for preparing products for detecting helicobacter pylori. The helicobacter pylori detection product provided by the invention has the advantages of being simple and convenient to operate, rapid in reaction, high in sensitivity, strong in specificity, suitable for on-site rapid detection, economical, practical, capable of identifying and typing and the like, and rapid screening, rapid, accurate and safe detection can be carried out.

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

The invention belongs to the technical field of application of natural products, and particularly relates to an anti-helicobacter pylori natural product composition and application, the anti-helicobacter pylori natural product composition comprises raspberry extract and cinnamon extract, and the composition contains anti-helicobacter pylori active substances. Besides, the natural product composition is combined with a clinical standard treatment method for treating helicobacter pylori, so that the curative effect can be further improved, and the gastrointestinal motility can be enhanced by adjusting intestinal flora disorder caused by pylorus infection, reducing toxic and side effects of antibiotic treatment, protecting gastric mucosa and enhancing gastrointestinal motility. The composition consists of two natural medicinal and edible plant extracts, is suitable for long-term use and has few adverse reactions. The Lycojaponicumin C can be used for preparing anti-helicobacter pylori medicines and preventive health-care foods, has obvious significance in prevention and treatment of helicobacter pylori infection, and has a good development prospect.

The invention discloses a compound preparation capsule and a preparation method thereof, wherein the compound preparation capsule is composed of Vonoprasone or a pharmaceutical salt microtablet thereof, an amoxicillin microtablet and a rifampicin or rifampetine microtablet. The Vonoprazan is a novel potassium ion competitive acid blocker, and the compound preparation capsule is used for treating gastric ulcer, duodenal ulcer, gastric MALT lymphoma, idiopathic thrombocytopenic purpura, and helicobacter pylori infection and helicobacter pylori infection gastritis in the stomach after early gastric cancer endoscopic therapy. According to the compound preparation capsule, the cure rate of helicobacter pylori can be increased, the drug resistance can be reduced, and a better treatment choice is provided for clinical patients.

The invention relates to a nano bismuth agent for preventing and / or treating gastritis infection as well as a preparation method and application thereof, the preparation method comprises the following steps: respectively dissolving soluble bismuth salt and a sulfur-containing compound, mixing, and reacting to obtain the nano bismuth agent after the reaction is finished, the sulfur-containing compound is any one or a combination of at least two of thioamino acid, vitamin B1, thioacetamide, sodium sulfide, sodium thiosulfate, bovine serum albumin, whey protein, ovalbumin or metallothionein. The prepared nano bismuth agent has efficient antibacterial performance, shows an excellent curative effect which is two orders of magnitude higher than that of an existing bismuth complex on clinically separated drug-resistant helicobacter pylori, and does not further induce increase of drug resistance of the helicobacter pylori.

Disclosed are an anti-Helicobacter pylori compound and use thereof, which relate to the technical field of pharmaceuticals. The compound is a compound represented by formula I and further comprises a stereoisomer thereof, a geometric isomer thereof, a tautomer thereof, a nitrogen oxide thereof, a hydrate thereof, a solvate thereof, a pharmaceutically acceptable salt thereof, or a prodrug thereof. The compound exhibits a good effect in the treatment of Helicobacter pylori infection.

The invention belongs to the field of biological medicine, and particularly relates to an anti-inflammatory and anti-helicobacter pylori compound separated from agilawood and a preparation method thereof. The method comprises the following steps: by taking the agilawood corasta as a raw material, carrying out reflux extraction with ethanol, extracting with petroleum ether to remove volatile components, separating and purifying by adopting various chromatographic technologies such as silica gel column chromatography, reversed-phase column chromatography and semi-preparative HPLC (High Performance Liquid Chromatography) to obtain a monomeric compound, and identifying the structure of the monomeric compound by adopting spectroscopy methods such as ultraviolet, high-resolution mass spectrometry and nuclear magnetic resonance. The structure of a compound 1 is identified as a new 2-(2-phenethyl) chromone dimer, and the compound 1 is named as aquicrasone V. The invention further discloses a preparation method of the compound 1. A biological activity test verifies that the compound has relatively strong anti-inflammatory activity and anti-helicobacter pylori activity.

The present invention provides three pairs of Helicobacter pylori antibodies and their applications, and defines the amino acid sequences of the heavy chain complementary determining regions and light chain complementary determining regions of the antibodies. The Helicobacter pylori antibodies provided by the present invention have good specificity, high biological activity, strong stability, and high affinity with Helicobacter pylori, and can be used to prepare products for detecting Helicobacter pylori. The Helicobacter pylori detection product provided by the present invention has the advantages of simple operation, rapid response, high sensitivity, strong specificity, suitability for rapid on-site detection, economical and practical identification and typing, etc., and can perform rapid screening, rapid, accurate, and safe detection.

The present invention provides a method for assessing the risk of suffering from gastric cancer in a patient from which Helicobacter pylori has been sterilized, the method comprising a step for detecting the expression of the KK-LC-1 gene in a gastric tissue sample derived from the patient before Helicobacter pylori has been sterilized, the expression of the KK-LC-1 gene is positive, and it is indicated that the patient has the risk of suffering from the gastric cancer after the helicobacter pylori is sterilized.

The invention discloses a transplanted tumor containing helicobacter pylori and a preparation method and application thereof, belongs to the technical field of biology, and further discloses a reagent for preparing a transplanted tumor tissue containing helicobacter pylori, and the reagent comprises tumor cells and helicobacter pylori and further comprises at least one of matrigel and defibrated sheep blood. The helicobacter pylori-containing transplanted tumor obtained by utilizing the reagent and the preparation method disclosed by the invention can be used for preparing a quality control substance or a reference substance in immunological detection, the HP thallus positive staining is stronger, and besides being used as positive quality control, the helicobacter pylori-containing transplanted tumor also has internal control of negative cell hematoxylin staining, so that the helicobacter pylori-containing transplanted tumor can be used for preparing the quality control substance or the reference substance. The same quality control product has both thallus positive staining and histocyte negative staining, has consistency with conventional case tissue detection, and can play a monitoring role in HP immunohistochemical quality control.

The invention relates to the technical field of stomach filling agents, and particularly discloses a stomach filling agent and a preparation method thereof. The stomach filling agent is prepared from, by weight, 50-75 parts of composite hydrogel, 1.7-2.8 parts of digestion promoting microcapsules and 0.7-1.2 parts of helicobacter pylori resisting matter, the composite hydrogel is formed by crosslinking sodium alginate and sulfhydrylated fructose-chitosan, the capsule wall of each digestion promoting microcapsule is gelatin and sodium alginate, and the capsule wall of each digestion promoting microcapsule is gelatin and sodium alginate. The core of the digestion promoting microcapsule is chitosanase; in addition, the preparation method disclosed by the invention has the advantages of reducing the stimulation of the gastric filling agent to gastric mucosa and improving the multifunctionality of the gastric filling agent.

The invention provides an innovative dual-mode detection method based on a dipeptide nano framework (DNPs), which is used for efficiently detecting helicobacter pylori. According to the method, firstly, a DNPs material formed by chelating dipeptide (Trp-Phe) and zinc ions (Zn (II)) is synthesized, and the material is decomposed along with the increase of the pH value in an alkaline environment, so that the Zn (II) is released; then, urea is hydrolyzed under the catalysis of urease to generate ammonia, so that the pH value of the solution is increased, and the decomposition of DNPs and the release of Zn (II) are further promoted. By utilizing an electrochemical detection mode, real-time analysis on urea and urease is realized by monitoring an electrochemical signal of Zn (II); meanwhile, in combination with the fluorescence characteristics of DNPs, the concentration of urea and urease is indirectly and quantitatively analyzed by monitoring the fluorescence intensity change. The dual-mode detection method combines the advantages of electrochemistry and fluorescence detection, has high sensitivity and real-time performance, provides an effective means for accurate detection of helicobacter pylori, and shows a wide application prospect in clinical diagnosis and sensing technologies.

The application provides a human Helicobacter pylori detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology, amplifies the genomic fragment containing the four-strand DNA sequence in the human Helicobacter pylori genome, and realizes rapid and visual detection of the human Helicobacter pylori by adding hematin and oxidoreductase substrate for color reaction.