231 results about "Enantiomer" patented technology

The invention relates to a high-efficiency near-ultraviolet heat exciton compound with circular polarization property and application of the high-efficiency near-ultraviolet heat exciton compound in preparation of a circular polarization organic electroluminescent device, and belongs to the technical field of organic photoelectric materials. The organic electroluminescent material is a high-efficiency near-ultraviolet heat exciton compound with circular polarization property, nine pairs of near-ultraviolet enantiomers are successfully designed and synthesized by combining chiral group binaphthol with fluorene with high luminous efficiency, and the nine pairs of near-ultraviolet enantiomers all show obvious circular polarization luminous property and high fluorescence quantum yield. The electroluminescent devices prepared based on the nine pairs of near ultraviolet enantiomers all show near ultraviolet emission, and the external quantum efficiency is 3.9%, 3.1%, 4.5%, 7.5%, 6.3%, 5.0%, 10.3%, 9.2% and 11.6% respectively. A new visual angle is provided for developing a near ultraviolet heat exciton material with circular polarization luminescence and high efficiency at the same time, and 3D and wide color gamut display can be directly achieved in the future.

The invention belongs to the field of detection and analysis, and relates to a method for separating and detecting enantiomers in (S)-2-amino-3-[2-(tert-butoxy)-2-oxoacetamido] tert-butyl propionate hydrochloride. According to the method, HPLC is adopted for separation and detection, a polysaccharide derivative chiral chromatographic column is selected as a chromatographic column, a mobile phase A is a weak base aqueous solution, and a mobile phase B is acetonitrile and / or methanol. The method is strong in specificity, high in separation degree, high in accuracy, stable, reliable and high in sensitivity, and a simple, accurate, rapid and reliable detection method is provided for control of impurities in a starting material of (S)-2-amino-3-[2-(tert-butoxy)-2-oxoacetamido] tert-butyl propionate hydrochloride.

The invention discloses a method for efficiently and accurately simulating three-dimensional electron diffraction dynamics intensity, and aims to solve the problems of low simulation calculation efficiency and insufficient precision in the prior art. According to the method, a reciprocal vector is rotated by adopting a Rodrigues formula through optimizing a reciprocal space sampling strategy, and a user-defined phase difference or random sampling is introduced in two dimensions of rotation and precession, so that the sampling frequency is remarkably reduced. And meanwhile, the diffraction intensity is accurately calculated by utilizing a Bloch wave method, and the diffraction intensity of the chiral crystal and the enantiomer thereof is calculated at one time by setting the negative thickness, so that repeated calculation is avoided. The invention supports a plurality of diffraction means, including electron diffraction automatic tomography (ADT), rotating electron diffraction (RED), precession electron diffraction tomography (PEDT), continuous rotating electron diffraction (cRED) and continuous rotating precession electron diffraction tomography (cPEDT), and can achieve convergence within 50, 100 and 500 times of sampling of single diffraction. According to the method, the calculation efficiency is improved, the application range of simulation is widened, and reliable technical support is provided for material structure analysis.

The invention discloses a high-color-purity near ultraviolet heat exciton compound with circular polarization property and application thereof in preparation of a circular polarization organic electroluminescent device, and belongs to the technical field of organic photoelectric materials. The electroluminescent material disclosed by the invention is a high-color-purity near ultraviolet heat exciton compound with circular polarization property, and two pairs of near ultraviolet enantiomers are successfully designed and synthesized by combining a chiral group binaphthol, a weak donor dibenzothiophene, dibenzofuran and an acceptor cyanophenyl; the compounds show obvious circular polarization luminescence property and high fluorescence quantum yield. The electroluminescent devices prepared based on the enantiomers all show near ultraviolet emission, CIE coordinates are respectively (0.168, 0.040) and (0.166, 0.040), and the external quantum efficiencies are respectively 4.3% and 3.4%. According to the near ultraviolet heat exciton material, a new visual angle is provided for developing the near ultraviolet heat exciton material with circular polarization luminescence and high color purity at the same time, and 3D and wide color gamut display can be directly achieved in the future.

According to the method, a polysaccharide derivative bonding type chiral chromatographic column with silica gel surface bonded amylose-tri (3, 5-dichlorophenyl carbamate) as a filler is adopted, a mixed solution of n-hexane and isopropanol is used as a mobile phase, the column temperature is 28-32 DEG C, the flow velocity is 1.1-1.3 ml / min, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, and the column temperature is 20-30 DEG C; an ultraviolet detector is adopted, the wavelength is 205-220 nm, and effective separation of the etomidate intermediate and the enantiomer thereof is achieved. The method is good in detection result accuracy and high in sensitivity, and compared with an existing analysis method, the method is better in separation effect, simpler in mobile phase composition, more durable in chromatographic column and lower in cost, so that a good guarantee is provided for quality control of the etomidate intermediate and etomidate.

The invention relates to a method and a system for high-sensitivity conformation detection of lactic acid enantiomers based on a terahertz quantum weak measurement spectrum technology and application, the method and the system are applied to a detection system, and the method comprises the following steps: a terahertz transmitting module outputs terahertz waves; the polarization state of the terahertz photons is pre-selected through the first polaroid to serve as a pre-selection state alpha of the quantum system; weak coupling interaction of terahertz waves and lactic acid enantiomers is realized between the pre-selection process and the post-selection process of a system state; a terahertz photon polarization state which is weakly coupled with the lactic acid enantiomer is screened by a second polaroid and is used as a post-selection state beta of the quantum system; the terahertz detection module receives the post-selected output signal; a terahertz quantum weak measurement spectrum transmission model is constructed, and terahertz wave intensity variation is calculated and output and reflects physical properties of lactic acid enantiomers. Through the arrangement, high-sensitivity conformation detection of the lactic acid enantiomer is realized.

The invention provides a method for determining the content of penicillamine enantiomers by pre-column derivatization liquid chromatography, namely a method for detecting L-penicillamine in D-penicillamine or a method for detecting D-penicillamine in L-penicillamine, derivatization is carried out under conventional alkaline conditions, a termination reaction is carried out by conventional acid, the derivatization steps are simplified, the detection efficiency is improved, and the detection cost is reduced. According to the method, various interferences of other amino acids and corresponding isomers in the raw materials can be effectively avoided due to the gradient of the mobile phase, the damage to a chromatographic column is well reduced due to the fact that conventional reagents such as phosphoric acid and acetonitrile serve as the mobile phase, and the method is easy to operate, high in sample preparation speed and high in accuracy. The method has the advantages of strong anti-interference capability, good separation effect, high sensitivity, accurate result and good stability, realizes effective separation of penicillamine isomers, has a separation degree of greater than 2, can perform content determination on another main drug while performing impurity detection on one isomer, and has good practicability.

The invention discloses a composite material modified electrode based on a functionalized single-walled carbon nanotube and application thereof, and belongs to the technical field of electrochemistry, the modified electrode comprises a glassy carbon electrode and a functionalized single-walled carbon nanotube-glucose composite material coating coated on the surface of the glassy carbon electrode; the mass ratio of functionalized single-walled carbon nanotubes to glucose in the coating is (0.2-5): 1, and the functionalized single-walled carbon nanotubes comprise at least one of hydroxylated single-walled carbon nanotubes, carboxylated single-walled carbon nanotubes and aminated single-walled carbon nanotubes, preferably hydroxylated single-walled carbon nanotubes. The composite material modified electrode provided by the invention has a large number of active sites, and by effectively utilizing the synergistic effect of the hydroxylated single-walled carbon nanotubes and glucose, the modified electrode shows excellent selective recognition capability on amino acid enantiomers and also shows good stability; and the method has a good application prospect in quantitative analysis of the amino acid enantiomers.

The invention discloses an enhanced Raman scattering substrate for chiral molecule detection and a preparation method, and relates to the technical field of spectral analysis and chiral recognition. Chiral cysteine is induced at the core of the gold nanorod to form a spiral chiral gold-silver alloy layer and then wraps the zirconium-based metal organic framework, the synthesized chiral gold rod (at) MOF material has excellent LPSR effect and chiral electromagnetic field, and the MOF layer on the surface can well adsorb molecules to be detected to enter a plasma hot spot area. The Raman substrate is excellent in SERS performance. According to the composite material, through the dual effects of molecular enrichment and electromagnetic enhancement, enantiomers are distinguished directly through SERS intensity, and labeling or catalytic conversion is not needed.

The invention discloses a dynamic tunable chiral terahertz broadband absorber based on vanadium dioxide, the absorber is formed by periodically arranging M * N absorption units of a multilayer structure in an array, the absorption units are in M * N two-dimensional distribution, and both M and N are positive integers; the absorption unit comprises a metal substrate, a dielectric layer and a resonance layer which are sequentially arranged from bottom to top; the metal substrate and the dielectric layer are rectangular; the resonance layer comprises a J-shaped metal patch, and an inverted-T-shaped vanadium dioxide patch is arranged at one end of the J-shaped metal patch. The terahertz chiral absorber can have a circular dichroism (CD) value larger than 0.8 within the range of 5.36-7.69 THz, the mirror image enantiomer of the terahertz chiral absorber can also achieve a CD spectrum opposite to the mirror image enantiomer, and the terahertz chiral absorber has the advantages of being high in CD, dynamically tunable and simple in structure. In addition, the invention also discloses a preparation method of the dynamic tunable chiral terahertz broadband absorber based on vanadium dioxide.

The invention provides a fullerene pyrrolidine enantiomer mediated cholesteric liquid crystal with circular polarization luminescence property and nonlinear anti-saturated absorption property, and a preparation method and application thereof. The method comprises the following steps: dissolving C60, paraformaldehyde and (R / S)-(+ / -)-alpha-methylbenzylamine in an organic solvent, and carrying out heating reflux reaction to obtain fullerene pyrrolidine enantiomer molecules; dissolving in carbon disulfide to obtain an enantiomer solution; dissolving achiral dye molecules in a solvent to obtain a dye solution; and dropwise adding the enantiomer solution and the dye solution into thermotropic liquid crystal molecules, and drying to obtain the thermotropic liquid crystal. The method is simple, the obtained cholesteric liquid crystal system shows a characteristic fingerprint texture and has a periodic spiral structure, and the doping proportion of chiral molecules is easy to adjust; the composite system has good time stability and is not prone to phase separation; the liquid crystal platform shows good circular polarization luminescence performance and third-order nonlinear optical property, and is a cholesteric liquid crystal platform with an integrated function.

Provided are a compound for recoverying a p53 mutation function and use thereof, particularly relating to a compound represented by Formula M, or an enantiomer, a diastereomer, a racemate, a tautomer, a stereoisomer, a geometric isomer, a crystal form, a nitrogen oxide, a metabolite or pharmaceutically acceptable salt, ester, solvate, hydrate, isotope-labeled compound or prodrug of the compound.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a detection method of an evocamide enantiomer, which comprises the following steps: S1, preparing a test solution; s2, preparing a contrast solution; s3, preparing a system applicable solution; s4, carrying out a system applicability test; s5, testing a test sample and a contrast solution; a chromatographic column is a chiral chromatographic column taking a polysaccharide derivative or a cellulose derivative as a filler, and a mixed solution of n-hexane, isopropanol, trifluoroacetic acid and diethylamine is taken as a mobile phase to detect the Evocadum enantiomer. The method has the characteristics of simplicity in operation, high sensitivity and high accuracy, the chromatographic peaks are high in separation degree, do not interfere with one another, the peak shape is good, the result accuracy is guaranteed, the method can be used for detecting enantiomers in the Evocamide and the Evocamide preparation, the level of the enantiomers of the Evocamide is effectively controlled, and the medication safety is guaranteed.

To provide a simple method for optically resolving a chiral compound, which is expected to be industrially applicable.SOLUTION: A method for optical resolution of a chiral compound includes dissolving a mixture of both enantiomers of the chiral compound in a first solvent to prepare a first solution, stirring the prepared first solution, precipitating a first gel in the stirred first solution, and separating the first solution into the first gel and a first supernatant.SELECTED DRAWING: Figure 1

The invention discloses an enantiomer crystallization resolution method of delta-dodecalactone, which comprises the following steps: hydrolyzing delta-dodecalactone under an alkaline condition for ring opening, acidifying to obtain 5-hydroxylauric acid, adding a dehydroabietylamine solution, dissolving in an alcohol solvent or a solvent system consisting of the alcohol solvent and other solvents, fully reacting, cooling, slowly crystallizing, filtering, washing, and drying to obtain the delta-dodecalactone enantiomer. Filtering, adding ethyl acetate and hydrochloric acid into a filter cake for dissolving, carrying out liquid-liquid extraction and washing an organic phase, and carrying out rotary evaporation concentration on the organic phase to obtain optically enriched delta-dodecalactone; the method is simple to operate and does not depend on expensive special equipment, and the sample loading amount is greatly increased; due to the introduction of dehydroabietylamine, the 5-hydroxylauric acid and the dehydroabietylamine form diastereoisomer salt, and chiral separation is carried out in the crystallization process; the reaction safety is high, and the requirements of green chemistry are met.

The invention belongs to the field of medicine synthesis, and particularly relates to a method for preparing fenerenone by recycling a fenerenone enantiomer. A brand new reaction route is designed, an enantiomer is subjected to photochemical catalysis to obtain an aromatization product, then in the presence of a specific catalyst, the aromatization product is subjected to dihydropyridine reduction to obtain a finelrenone racemate, and the finelrenone racemate is subjected to resolution, dissociation and recrystallization to obtain the finelrenone. Compared with the prior art, the method has the advantages that the fennerenone enantiomer is recycled, the recycled fennerenone enantiomer is used for preparing the fennerenone, compared with the prior art, photochemical catalysis is realized under the action of the specific catalyst, the reaction condition is mild, the operation is simple, the requirement on equipment is low, and the method is suitable for industrial production. According to the method, the recycling of the enantiomer of the fenerenone can be realized for industrial production of the fenerenone, the commercialization cost of the fenerenone is reduced, large-scale industrial production can be carried out, repeated recycling is realized, and a direction is provided for process improvement of the fenerenone.

The invention discloses a method for identifying amino acid enantiomers by using an SERS-Cha substrate. The method comprises the following steps: preparing a chiral silver nano spiral structure by using an electron beam evaporation coating instrument in combination with a liquid nitrogen low-temperature system; on the basis of the SERS-Cha theory, the chiral substrate is used for performing SERS characterization on two chiral histidine molecules, and the chiral histidine molecules with two configurations are identified according to the significant difference of SERS intensities of enantiomer molecules on the same chiral platform. The method provided by the invention is different from the traditional method for detecting the chiral enantiomer by circularly polarized light, realizes the identification of the chiral enantiomer by linearly polarized light, and is an effective and simpler method.

An unnatural amino acid, where the unnatural amino acid is a compound with a structure as shown in formula (I) or an enantiomer thereof,where X represents —O—, —S—, —NH— or —CH2—, Y represents —O—, —S—, —C(O)—, —S(O)— or —CH2—, and L represents substituted or unsubstituted C0-C20 linear or branched alkylene, where one or more —CH2— are optionally substituted by one or more of —O—, —S—, —NH—, —C(O)— and —S(O)—, when X represents —O— and Y represents —C(O)—, L does not represent —CH2CH2—, and when L represents a substituent, the substituent is selected from one or more of hydroxyl, sulfhydryl, halogen, nitro, cyano, alkyl, alkenyl, alkynyl, alkoxy, acyl, acylamino, carboxyl, ester, amino, sulfonyl, sulfinyl, cycloalkyl, heterocyclyl, aryl and heteroaryl.

The present invention provides a method for detecting and identifying molecular chirality based on a composite plasmon chiral nanostructure. The present invention constructs a composite plasmon chiral nanostructure using chiral nanofibers and gold nanorods as basic components, and achieves molecular chirality recognition and detection through plasmon chiral optical amplification. The excellent plasmon circular dichroism optical properties of the composite nanostructure can convert the host-guest chiral recognition interaction occurring at the fiber interface into an asymmetric plasmon chiral optical amplification signal. This technology can detect and identify the chiral enantiomers of amino acid molecules in about 1 minute, with a sensitivity of 10 ‑6 mol / L. This structure can also be used for quantitative analysis of molecular enantiomeric excess (ee), with a detection sensitivity of ±0.05. Furthermore, given that the chiral host-guest complexes involved in this technology are primarily based on hydrogen bonding interactions, the chiral fiber host can be used as a universal substrate for the detection of chiral amino acid molecules.

This invention belongs to the field of membrane separation and functional membrane materials technology, and discloses a chiral graphene oxide-based separation membrane, its preparation method, and its application. The membrane is composed of graphene oxide and / or reduced graphene oxide sheets and a single-stranded DNA-single-walled carbon nanotube complex. The single-stranded DNA helically winds around the outer surface of the single-walled carbon nanotubes to form a one-dimensional chiral nanochannel and intercalates between the sheets, forming a three-dimensional chiral channel network connected in the membrane thickness direction. The preparation method includes: heating and decohexing salmon sperm double-stranded DNA in a urea-containing buffer to obtain single-stranded DNA; dispersing single-walled carbon nanotubes and then complexing them with the single-stranded DNA; further mixing with a graphene oxide or reduced graphene oxide dispersion; and vacuum filtration to form the membrane. This membrane exhibits a high separation factor for chiral enantiomers such as L / D-phenylalanine, with an excess value of up to 99.95% for L-phenylalanine enantiomers after multi-stage separation, making it suitable for the efficient separation of chiral drugs and amino acids.

The invention relates to a method for analyzing and detecting a non-azonetin enantiomer, which adopts normal-phase high performance liquid chromatography and takes normal hexane-ethanol-trifluoroacetic acid as a mobile phase to detect the non-azonetin enantiomer, and the specific chromatographic conditions are as follows: a chromatographic column is prepared by covalently bonding amylose-tri (3, 4, 6-trimethyl-1, 3-pentanediol) on the surface of silica gel; 2, 5-dichlorophenyl carbamate is used as a filling agent (IE, 4.6 * 250 mm, 5.0 [mu] m); a mobile phase: n-hexane: ethanol: trifluoroacetic acid = 500: 500: 1 (V / V / V); the flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the sample size is 20 [mu] l; the detection wavelength is 280 nm; the elution gradient is isocratic elution. The invention provides a high performance liquid chromatography method for analyzing the enantiomer of the fiazonetin, the complete separation of the fiazonetin and the enantiomer of the fiazonetin can be realized by adopting the method, and the separation degree of a main component and the enantiomer is greater than 1.5.

The present invention belongs to the field of organic chemical ligands and discloses a nitrogen-nitrogen-phosphorus ligand having a structure of the general formula I or its tautomers, enantiomers, and diastereomers: #imgabs0#R 1 、R 5 R is independently selected from hydrogen, alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, hydroxyl, aldehyde, carboxyl, ester, halogen, trifluoromethyl, cyano, acyl, amide, amino, nitro, phenyl, benzyl, sulfonyl, sulfonic acid, and mercapto; 2 is selected from one of the following structures: alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, phenyl, benzyl, phenethyl, naphthylmethyl, -CHPh2, ester, -CH2OBn, or substituted phenyl or benzyl, or R 2 With R 4 connected to form a five-membered ring and an acene ring; the R 3 is selected from hydrogen or alkyl; said R 4 is selected from hydrogen, alkyl, alkoxy, alkenyl, alkynyl, phenyl, benzyl; W is PR2 or P(O)R2, and R is selected from phenyl, naphthyl, cyclohexyl, and substituted phenyl. The present invention also discloses a preparation method and use of nitrogen-nitrogen-phosphorus ligands.

The invention discloses a spiral chiral gold nanorod as well as a preparation method and application thereof, and the method comprises the following steps: (1) sequentially adding hexadecyl trimethyl ammonium bromide, cysteine and p-aminothiophenol into an anisotropic gold nanorod solution, uniformly mixing, and adsorbing; and (2) silver nitrate, chloroauric acid and ascorbic acid are added for a reaction, centrifugal dispersion is performed after the reaction is finished, and the spiral chiral gold nanorod is obtained. Dispersing the spiral chiral gold nanorod in an aqueous solution, adding phenylalanine enantiomer, dropwise adding on a clean glass slide after incubation is finished, and performing surface enhanced Raman spectroscopy test by using a Raman microscope after air drying. According to the invention, the broadband coverage of chiral response from visible light to near ultraviolet region is realized by accurately regulating and controlling the arrangement of interface molecules and the morphology of the nano structure.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a multifunctional chiral selection reagent solution (CSS) and application of the multifunctional chiral selection reagent solution. More specifically, the invention relates to a multifunctional chiral selection reagent solution for chiralMS (chiralMS) analysis, and the solution is prepared from CuCl2, D-Phe, L-Ph-d5 and D-or L-3'and / or 4 'hydroxyl substituted Kelin lactone. According to the present invention, the on-line non-covalent derivatization can be performed on the enantiomers in the complex system sample by using the multifunctional chiral selection reagent solution and by using the post-column injection liquid chromatography-mass spectrometry (post-column injection liquid chromatography-mass spectrometry / MS, PCI-LC-MS / MS) system so as to achieve the qualitative and quantitative analysis of the enantiomers and the rapid screening of the chiral selection reagent (CS).

The application belongs to the technical field of analysis and detection, and discloses a chiral detection platform based on a hydrogel SERS chip and application thereof.The core of the application is that the inherent chiral structure of a hydrogel matrix in a specific hydrogel SERS chip is found and utilized for the first time, so that specific enantiomers of chiral molecules can be directly and highly selectively recognized and detected without any exogenous chiral modifier or complex separation process.When the chip is used for detection with levomeprazole as a model analyte, only levomeprazole can be selectively adsorbed and produce a strong characteristic SERS signal, and the response of the dextrogyratory enantiomer is weak under the optimal conditions of an acid medium with pH 4.The sensor constructed by the method exhibits high sensitivity to levomeprazole, and the detection limit reaches 1.0x10 ‑7 mol / L, excellent selectivity and good anti-biological matrix interference capability, thereby providing a novel, simple and efficient solution for rapid analysis of chiral drugs.

The invention discloses a method for directly preparing a racemic oxidation product from a fennerenone enantiomer through photocatalysis, and belongs to the technical field of organic synthesis.The method comprises the steps that an R-type fennerenone isomer is taken to be placed in a reaction bottle, a photosensitizer containing iridium or ruthenium is added, and a solvent is added at the speed of 0.1 M in the air atmosphere; the method comprises the following steps: dissolving 4-(4-cyano-2-methoxyphenyl)-5-ethoxy-2, 8-dimethyl-1, 6-naphthyridine-3-formamide in a solvent, stirring, transferring a mixture into a photoreactor of a blue light LED (Light Emitting Diode) with the wavelength of 455-465 nm, stirring, transferring a mixed solution after the reaction is finished into a round-bottom flask, purifying a volatile solvent to obtain a product 4-(4-cyano-2-methoxyphenyl)-5-ethoxy-2, 8-dimethyl-1, 6-naphthyridine-3-formamide, and carrying out chiral HPLC (High Performance Liquid Chromatography) analysis on the product. High temperature, high pressure or a strong oxidant is not needed, and reaction conditions are mild; oxygen in air is oxidized at room temperature, so that energy consumption and pollutant generation are reduced; compared with the traditional oxidation condition, the method does not need to undergo the process of oxidation first and then high-temperature induced racemization, and the racemization product can be directly obtained.

The invention discloses a planar chiral [2.2] paracyclophane alkane monophosphine ligand pEPCP as well as a preparation method and application thereof, and relates to the technical field of chemical catalysis, the planar chiral [2.2] paracyclophane alkane monophosphine ligand pEPCP is characterized by comprising a racemate or an enantiomer, and the structural formula I of the monophosphine ligand pEPCP is as follows: # imgabs0 #. Different types of ether substituents are introduced into the structure of a unique large-steric-hindrance electron-rich rigid skeleton formed by connecting two benzene rings at para-positions through two ethylidene groups, so that the electron cloud density and steric hindrance of aromatic rings directly connected with phosphine ligands are changed. In addition, the ether substituent can also be used as a weak coordination group and participates in adjusting a dihedral angle of ligand chelation. The metal, the ligand and the substrate form the most appropriate coordination environment in the catalysis process, so that the corresponding catalytic reaction has the advantages of high reaction activity, good enantioselectivity, wide substrate universal application range and the like, is particularly suitable for asymmetric coupling reaction catalyzed by transition metal, and has economic practicability and industrial application prospects.

The application discloses a kind of plane chiral [2.2] paracyclophanane monophosphine ligand pEPCP and preparation method and application thereof, it is related to chemical catalysis technical field, and its technical scheme main points are as follows: including racemate or enantiomer, the structural formula-I of the monophosphine ligand pEPCP of the application is as follows: [2.2] paracyclophanane skeleton containing monophosphine ligand pEPCP of the application, with two benzene rings in para position by two ethylene connection unique big steric hindrance, electron-rich rigid skeleton, different types ether substituent group is introduced in structure, change the electron cloud density and steric hindrance of aromatic ring directly connected by phosphine ligand.In addition, ether substituent group can also be used as weak coordination group, participate in adjusting ligand chelate dihedral angle.This makes metal, ligand and substrate form the most suitable coordination environment in catalytic process, so that corresponding catalytic reaction has the advantages such as high reactivity, good enantioselectivity, substrate universal range is wide, especially suitable for transition metal catalyzed asymmetric coupling reaction, with economic practicability and industrial application prospect.

The invention discloses a normal-phase chromatographic analysis and detection method for a tiagonium bromide intermediate and an enantiomer of the tiagonium bromide intermediate, and belongs to the technical field of analytical chemistry. The method comprises the following steps: preparing a system applicability solution, a test solution and a contrast solution; setting liquid chromatography detection conditions, and adopting a normal phase chromatographic column with cellulose-tri (3, 5-dimethyl phenyl carbamate) coated silica gel as a filling agent; finally, the system applicability solution, the test solution and the contrast solution are taken and injected into a liquid chromatograph, and chromatograms are recorded. The detection method can be used for rapidly detecting the content of enantiomer impurities in the tiagradionium bromide intermediate, is high in specificity and separation degree, and can be used for well separating an R-configuration tiagradionium bromide intermediate and an S-configuration enantiomer impurity; the method has the advantages of high sensitivity and good repeatability, and can accurately quantify the content of enantiomer impurities in the tiagradionium bromide intermediate.

The capabilities of gas or liquid chromatography-molecular rotational resonance (GC / LC-MRR) instrumentation exceed those of high-resolution mass spectrometry and nuclear magnetic resonance in terms of selectivity, resolution, and compound identification. MRR detection provides high specificity for selective gas or liquid phase separations, including the ability to resolve co-eluting peaks and isomeric compounds without any loss of specificity or accuracy. MRR allows for qualitative identification and absolute quantification of analyte components separated by GC or LC in the absence of reference standards. GC-MRR is ideal for compound-specific isotope analysis (CSIA) and can identify enantiomers and enantiomeric excess. GC-MRR measurements are particularly useful for studying biosynthesis / degradation and geochemical isotopic compounds.