2350 results about "Growth factor" patented technology

In various embodiments, the disclosure provides compositions, such as polypeptides, polynucleotides, gene editing systems, small molecules, vectors, or host cells, comprising and / or modulating the expression or activity of an immunomodulatory-related protein. In various embodiments, the disclosure also provides for the treatment of aging, senescence, fibrosis, metabolic diseases, cardiovascular diseases, endocrine-related disorders, genetic diseases, cancer, infections, immune diseases, and the like using agents comprising and / or modulating the expression or activity of an immunomodulatory-related protein. Methods of replacing an adaptation of therapy or combinations thereof with hormones, growth factors and / or proteins, as well as methods of identifying the agents.

The invention provides an application of bifidobacterium animalis subsp. Lactis CP-9 in preparation of a product for promoting bone growth and development or improving bone health. Long-term research and a large number of experiments show that the bifidobacterium animalis subsp. Lactis CP-9 can improve the bone volume fraction, the bone trabecula thickness, the bone density and the bone volume of an organism, and can also effectively improve the growth hormone level and the insulin-like growth factor 1 level, so that bone growth and development are promoted. Meanwhile, the bifidobacterium animalis subsp. Lactis CP-9 can effectively improve the N-terminal propeptide level, osteocalcin level, bone morphogenetic protein level and osteoprotectin level of I-type procollagen in a body, and remarkably reduce the amino-terminal peptide level of the I-type collagen and the ligand level of nuclear factor kappa B receptor activating factor, so that osteoblast differentiation is promoted, osteoclast differentiation is inhibited, and osteoclast differentiation is inhibited. Furthermore, bone formation is promoted, bone resorption is inhibited, bone metabolism is improved, and finally the effect of improving bone health is achieved.

The application relates to a compound of formula (I) containing a thiazole ring, an indazol ring and a 6,7-dihydro-5H-pyrrolo[1,2-c]imidazole ring system, to pharmaceutical compositions containing it and its medical use. The compounds are described as selective allosteric inhibitors of T790M / L858R, T790M / L858R / C797S, L858R, L858R / C797S containing EGFR mutants and thus useful for the treatment of cancer, in particular non-small cell lung cancer.

The invention discloses an abdominal aortic aneurysm progress prediction method and system based on cross-modal knowledge distillation. The method comprises the following steps: collecting blood of a target object; the method comprises the following steps: detecting the concentration of blood protein fingerprint markers of blood of a target object, wherein the blood protein fingerprint markers comprise matrix metalloproteinase-12, calcitonin-related polypeptide-alpha, uromodulin, nerve injury induction protein-1, glycosylated hemoglobin A1c and fibroblast growth factor-9; carrying out abdominal aortic aneurysm risk calculation by calling Student-net of cross-modal knowledge distillation based on the blood protein fingerprint marker concentration; wherein the input of the Teaser-net of the cross-modal knowledge distillation comprises a CTA image feature and a pathological section feature, and a category probability vector output by the Student-net is aligned with a category probability vector output by the Teaser-net; and outputting the risk level of the abdominal aortic aneurysm and the probability of occurrence / progress / rupture of the abdominal aortic aneurysm in the preset year. And the development of the aortic aneurysm disease is accurately predicted.

The invention relates to the technical field of biological medicine, in particular to a wound repair material and a preparation method and application thereof.The wound repair material is in a gel shape and comprises hydrogel and platelet-rich plasma, the hydrogel is formed by dynamic crosslinking of collagen and oxidized hyaluronic acid, and the hydrogel physically encapsulates the platelet-rich plasma. The gelatinous wound repair material provides bionic mechanical support through a dynamic cross-linked network, synergistically regulates and controls an inflammation microenvironment and sequential release of PRP growth factors, remarkably promotes angiogenesis and wound healing, is suitable for the clinical treatment fields of diabetic ulcer, chronic difficult-to-heal wounds and the like, and has broad application prospects. The material has the functions of mechanical support, anti-inflammatory regulation and growth factor controlled release.

The invention relates to anti-drug neutralizing antibody analysis. In particular, the present application relates to methods for detecting neutralizing antibodies against a vascular endothelial growth factor (VEGF) binding moiety and a programmed cell death ligand-1 (PD-L1) binding moiety in a biological sample from an individual administered a bispecific antibody drug comprising the VEGF binding moiety.

The invention relates to the technical field of medical dressings, in particular to a perianal drug dressing of a multi-layer flow guide composite fabric and a preparation method and application of the perianal drug dressing of the multi-layer flow guide composite fabric. The perianal drug dressing is of a four-layer structure, a wet tissue adhesion layer is made of polydopamine modified chitosan / oxidized hyaluronic acid hydrogel, and wet-state adhesion is achieved; the drug sustained-release layer is used for dispersing mesoporous silica loaded with ciprofloxacin hydrochloride / defensin and chitosan microspheres loaded with epidermal growth factors / lonicera japonica into a polycaprolactone fiber membrane to achieve synergistic antibacterial and healing promotion; the directional flow guide layer is of a gradient wettability sandwich structure with a bionic micro-channel, and one-way rapid drainage can be achieved; the high-absorption outer layer is carboxymethyl cellulose / sodium polyacrylate composite sponge, liquid is locked efficiently, the wound surface is kept dry and comfortable, the clinical problem in perianal infection wound treatment can be effectively solved, and the treatment effect and the patient comfort are remarkably improved.

Described herein are methods of treating or reducing the severity of thyroid eye disease (TED), as well as antibodies that bind and inhibit signaling through the insulin-like growth factor receptor 1, useful in the methods.

The invention discloses a vascular endothelial growth factor (VEGF) and antibacterial peptide (AMP) loaded chitosan methacrylic acid (CSMA) hydrogel microneedle patch as well as a preparation method and application thereof, the microneedle patch takes CSMA hydrogel as a main body material, the CSMA hydrogel is crosslinked and cured through ultraviolet light, and the microneedle patch has the characteristics of simplicity and convenience in preparation and stable material performance. The microneedle patch can continuously and stably release VEGF and AMP, promote wound healing and inhibit bacterial infection. Experiments show that the hydrogel microneedle is non-toxic to cells, has good biocompatibility and biodegradability, and also has excellent antibacterial performance. The invention provides an efficient and safe novel treatment tool for repairing infectious wounds.

The invention discloses a titanium-based hydroxyapatite composite coating and a preparation method thereof. The problems that a traditional coating is weak in interface bonding, single in porosity and mismatched in drug sustained release are solved. The composite coating comprises a gradient component layer and a bionic porous outer layer which are sequentially stacked on the surface of a titanium substrate, wherein the gradient component layer comprises a bottom layer (a titanium-based hydroxyapatite transition layer, 70-80% of HA and bonded through Ti-O bonds) and a middle layer (an HA / bioglass composite layer, 50-70% of HA and 10-20% of bioglass); the bionic porous outer layer is a porous HA layer, the porosity is 40-60%, the porosity is gradually increased in a gradient mode in the direction away from the titanium substrate, surface layer pores are loaded with anti-infection drugs, and inner layer pores are loaded with bone growth factors. The method realizes improvement of the interface bonding strength of the coating, consideration of mechanical and biological activity and staged slow release of the medicine, and is suitable for the field of bone joint implantation.

The invention discloses a low-carbon freshwater fish feed added with fermented bamboo powder and application of the low-carbon freshwater fish feed, and belongs to the technical field of aquatic animal feeds. The low-carbon freshwater fish feed capable of reducing the fat rate of fish bodies and / or reducing nitrogen and phosphorus emission is prepared from the fermented bamboo powder, and the content of vitamins, enzymes and growth factors in the feed can be increased by adding the fermented bamboo powder, so that the freshwater fish bodies have good anti-pathogenic activity; the absorption and utilization of the freshwater fish on nutritional ingredients and fiber-rich ingredients in the feed are improved, so that the aim of reducing the emission of nitrogen and phosphorus is fulfilled; meanwhile, the fermented bamboo powder is used for partially replacing soybean meal in the freshwater fish feed, so that the dosage of feed raw materials such as the soybean meal is reduced, the intestinal health of the freshwater fish is improved, the muscle quality of the freshwater fish is improved, the earthy smell in muscles is reduced, the body type of the freshwater fish is also improved, and the fat content in abdominal muscles is reduced.

Disclosed are methods, means, and compositions of matter useful for the stimulation of angiogenesis directly by administration of membrane vesicles, such as fibroblast-derived exosomes, and / or through induction of angiogenic cytokines from blood cells contacted with fibroblast-derived exosomes. The invention provides means of treating conditions in which angiogenesis is beneficial through local or systemic administration of exosomes, including those derived from fibroblasts, wherein the fibroblasts are cultured under basal conditions or conditions of hypoxia. In other embodiments exosomes derived from fibroblasts are utilized to augment endogenous regenerative processes, such as hematopoiesis, angiogenesis and neurogenesis, as well as augment regenerative processes stimulated by administration of exogenous therapeutics such as cells, growth factors, or genes.

The invention relates to medical recombinant collagen repair gel and a preparation method thereof, and belongs to the technical field of recombinant collagen. Through the synergistic effect of the collagen, the chitosan, the polylactic acid, the nanocellulose, the vascular endothelial growth factor, the anti-inflammatory drug and the cell adhesion peptide, the tissue repair effect is remarkably improved. Through a gas foaming method, staged freezing and freeze drying, multistage pore size distribution is generated, gel with a porous structure is formed, and cell permeation and tissue regeneration are facilitated; in addition, the permeability and the drug loading capacity of the gel are improved, the cell adhesion peptides, the growth factors and the anti-inflammatory drugs are better adsorbed to the gel, functional slow release is achieved, and the repairing effect is further improved.

The invention relates to an external composition for hair growth regulation and a preparation method thereof, and belongs to the technical field of cosmetics and medicines. The composition disclosed by the invention comprises nano mesoporous carbon for enhancing permeation, various surface functional modifications on the nano mesoporous carbon, minoxidil, protein fragments, polypeptides, oligopeptides, amino acids, dutasteride, bimatoprost, traditional Chinese medicine extracts, vitamins, caffeine, nucleosides, growth factors and various auxiliary functional components. Wherein the pore diameter of mesopores of the nano mesoporous carbon for enhancing permeation is 2-30 nm, the total pore volume of the mesopores is 1.5-3.5 cm < 3 > / g, and the particle size of the nano mesoporous carbon is 50-200 nm. The prepared nano mesoporous carbon is granular, controllable in size, stable in structure, free of irritation to skin, capable of keeping inert to various active ingredients in the composition, capable of enhancing absorption and permeation of other ingredients on the skin, wide in application range, capable of compounding various types of active ingredients and high in biological safety.

The invention discloses a stem cell exosome based on targeted fusion polypeptide modification, a preparation method and application of the stem cell exosome in treating male erectile dysfunction. According to the exosome, a specific targeting fusion polypeptide (sequence: EPLQLKM-GGGS-DPPV) is anchored on the surface through a chemical coupling technology, so that the targeting property of the exosome on cavernosum smooth muscle cells is enhanced; meanwhile, an anti-TrkA monoclonal antibody (TrkA-2E7-HL) is loaded through an electroporation method, and a fibrosis pathway mediated by a nerve growth factor (NGF) is inhibited. The exosome induces secretion of the umbilical cord mesenchymal stem cells by adopting an anti-oxidation culture medium, so that the anti-oxidation activity of the exosome is remarkably improved. Experiments show that in a diabetes ED rat model, the erectile function (ICP / MAP ratio) of the modified exosome is improved to 0.79 + / -0.05, the fibrosis area is reduced to 9.2 + / -1.6%, and the NO secretion level is improved by 3.5 times compared with that of a common exosome. Through triple mechanisms of polypeptide targeting, antibody synergy and oxidation resistance, effective reversion of the ED pathological process is realized, and the polypeptide has remarkable novelty and clinical transformation potential.

The invention discloses a pharmaceutical composition for promoting aTreg cell differentiation and application thereof, and relates to the field of biological medicine, and the pharmaceutical composition comprises zinc salt, a basic cell culture medium, fetal calf serum, antibiotic, L-glutamine and a pH buffer agent. According to the application, the basic cell culture medium is used for providing basic nutrition required by cell growth for lymphocytes, the fetal calf serum is used for providing growth factors, hormones, adhesion proteins and nutrient substances required by cell growth for the lymphocytes, and the antibiotics are used for preventing bacterial infection; l-glutamine provides key amino acid for cell energy metabolism and participates in synthesis of protein and nucleic acid, zinc salt provides zinc ions, and under the comprehensive action, the proportion of Treg cells and aTreg cells differentiated from lymphocytes in peripheral blood mononuclear cells is increased.

The invention relates to the technical field of stem cell injections, in particular to a human umbilical cord mesenchymal stem cell injection and a preparation method thereof. The cell cryoprotectant comprises the following components: a cell cryoprotectant and P6-generation human umbilical cord mesenchymal stem cells. By selecting P6-generation human umbilical cord mesenchymal stem cells, the capacity of secreting hepatocyte growth factors (HGF) can be improved; the cell cryoprotectant contains 6-9wt% of dimethyl sulfoxide and a certain amount of human serum albumin, can better protect cells and maintain the motility rate and biological functions of the cells, and is more beneficial to preparation of cryopreserved human umbilical cord mesenchymal stem cell injection. The P6-generation human umbilical cord mesenchymal stem cells are obtained by culturing a culture medium containing fetal calf serum, and the ability of the injection to repair liver injury can be improved.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

The invention relates to the technical field of repair materials, and particularly discloses an extracellular matrix fiber material as well as a preparation method and application thereof. The invention discloses a preparation method of an extracellular matrix fiber material. The preparation method comprises the following steps: taking animal tissues, and carrying out virus inactivation, decellularization and antigen removal, degreasing, drying, grinding and sterilization; the decellularization and antigen removal method comprises the following specific steps: sequentially soaking a sample with a surfactant solution for h; then, soaking treatment is carried out by adopting a salt-alkali solution which contains 0.05-3 mol / L of salt and has the pH value of 8-11; then carrying out soaking treatment by adopting a 0.1-1wt% enzyme solution; and in degreasing, one or more of normal hexane, trichloromethane, dichloromethane, methanol, ethanol, acetone, diethyl ether and ethyl acetate are used for soaking the sample. According to the technical scheme, the ECM powder particle material with high growth factor content is prepared.

The invention relates to a regenerated decellularized adipose derivative as well as a preparation method and application thereof. The preparation raw material of the regenerated decellularized adipose derivative is prepared from a vascular matrix component (SVF) separated from human adipose tissue through decellularization treatment and freeze drying; the prepared regenerated decellularized adipose derivative is a porous material, the porosity is greater than 90%, and the aperture is 50-200 [mu] m; the composition comprises the following components in percentage by weight: 60-70% of collagen, 15-20% of elastin, 1 * 10 < 10 >-5 * 10 < 10 > particles / g of exosome, less than or equal to 5 ng / mg of DNA (deoxyribonucleic acid) residue, more than or equal to 30 ng / g of VEGF (vascular endothelial growth factor) and more than or equal to 15 ng / g of FGF-2 (fibroblast growth factor). The prepared regenerated decellularized adipose derivative is low in DNA residual quantity, rich in growth factors and capable of meeting the requirement for deep tissue filling due to the fact that the collagen content and the elastin content are high.

The invention provides a composition for beautifying, which comprises a blood platelet concentrated solution prepared by differentiation of human pluripotent induced stem cells (iPSC), and an additive for preparing a blood platelet suspension or gel, and is characterized in that the composition for beautifying comprises the following components: a blood platelet concentrated solution prepared by differentiation of human pluripotent stem cells (iPSC), and an additive for preparing the blood platelet suspension or gel. The invention aims to provide a functional platelet-rich composition which can be prepared in a large scale, is stable in batch and can be produced in a standardized manner in the aspect of promoting wound healing. According to the composition, the platelet concentrate and platelet-rich plasma (PRP) separated from blood in the composition are completely different in component, the component is stable, and the slow-release growth factor is beneficial to tissue repair.

The invention provides an efficient fermentation production method and application of acid type sophorolipid, and the production method comprises the following steps: (1) in a growth stage, adding a composite nitrogen source and growth factors into a basic culture medium to promote the growth of zymophyte and realize high-density fermentation, the zymophyte being candida utilis subjected to genetic engineering modification; (2) in the product synthesis stage, mixed syrup and vegetable oil are used as carbon sources, and the concentration of glucose in a fermentation tank is maintained at 1-5g / L and the concentration of residual oil is maintained at 1-5g / L by regulating and controlling the material supplementing rate. The fermentation production method provided by the invention solves the problems of slow thallus growth, long fermentation period, low production intensity, uncontrollable product composition, more byproducts, peculiar smell of fermentation tail gas and the like in the existing sophorolipid production process, and has industrial application value.

The invention discloses an anti-neurodegeneration exosome composition as well as a preparation method and application thereof. The anti-neurodegeneration exosome composition is prepared from the following components in parts by mass: 5 to 25 parts of human exfoliated deciduous tooth stem cell exosome, 25 to 85 parts of plant-derived exosome-like nano-vesicles, 12 to 10 parts of ginsenoside Rg, 2 to 10 parts of curcumin and 10 to 20 parts of auxiliary materials. The anti-neurodegeneration exosome composition contains a large amount of cell factors, growth factors, miRNA, mRNAs and other components, is easy to fuse with cell membranes of intestinal epithelial cells, can selectively deliver bioactive substances to recipient cells through a blood-brain barrier, carries out information transmission among different cells, regulates signal transduction among the cells, and has a good anti-neurodegeneration effect. The effects of resisting oxidation, resisting inflammation, resisting aging and the like are achieved.

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

The invention provides a diagnostic marker for systemic lupus erythematosus and application of the diagnostic marker, and belongs to the technical field of biological medicine. The diagnostic marker is at least one protein of PDGFA (Platelet Derivative Growth Factor Amplification), APOD (Adenosine Polymerase Chain Reaction), CFHR5, AMDHD1 and SCGB3A1; the amino acid sequence of the PDGFA is as shown in SED ID NO. 1; the amino acid sequence of the APOD is as shown in SED ID NO. 2; the amino acid sequence of the CFHR5 is as shown in SED ID NO.3; the amino acid sequence of AMDHD1 is as shown in SED ID NO. 4; and the amino acid sequence of the SCGB3A1 is as shown in SED ID (Sequence of Identification) NO.5. According to the present invention, the five proteins are adopted as the SLE disease diagnosis biomarker independently or in the combination manner, such that advantages of high accuracy, high sensitivity and high specificity are provided, and the new target spot is provided for the diagnosis, the intervention improvement and the like of the SLE patient.

The invention relates to the technical field of biomedical engineering, and discloses a preparation method of a nano-enzyme SERS (Surface Enhanced Raman Scattering) sensor for detecting diabetic retinopathy, which comprises the following steps: (1) synthesizing a bimetallic gold-palladium nanorod; (2) assembling a gold trioctahedron array; and (3) modifying the surface of the gold-palladium nanorod synthesized in the step (1) with single-stranded DNA1 to prepare a nano-enzyme probe, hybridizing an aptamer of a vascular endothelial growth factor (VEGF) with single-stranded DNA2, modifying the surface of the gold trioctahedron array assembled in the step (2) with the hybridized aptamer, preparing a capture substrate, and constructing the nano-enzyme SERS sensor by the nano-enzyme probe and the capture substrate. In the detection process, the VEGF triggers a competitive recognition mechanism to promote the nano-enzyme probe to be captured by the capture substrate and catalyze tetramethyl benzidine (TMB) to generate oxTMB with a strong SERS signal. The sensor has the advantages of high sensitivity, strong specificity and good accuracy, and can realize rapid quantitative detection of DR biomarkers in serum.

Antibodies and compositions against Insulin-Like Growth Factor-1 Receptor (IGF-1R) and uses thereof for treating thyroid eye disease (TED) are provided herein. Anti-IGF-1R antibodies and compositions described herein can be used in methods of treating or reducing the severity of thyroid eye disease (TED) in subjects in need thereof, by inhibiting the activity of IGF-1R with such antibodies and compositions.

The present invention relates generally to compositions, formulations, and methods for immunotherapy and drug delivery, including the treatment of eye diseases such as dry eye disease (DED). In particular, the present invention relates to methods of producing exosomes from mesenchymal stem cells (MSC-Exos) and, optionally, loading said exosomes with one or more bioactive substances. The exosomes may be loaded using electroporation with one or more bioactive substances such as proteins, miRNAs, and / or siRNA. In specific embodiments, the exosomes, and / or one or more biological compounds derived from the exosomes, may be provided to an individual in need thereof, including as part of one or more compositions containing mesenchymal stem cells (MSCs). The individual in need thereof may be an individual having a medical disorder such as DED, immune disorders, cancer, and other disorders. Therefore, the compositions containing MSCs can be used for topical application to the eye. The compositions can contain MSCs, MSC-Exos, one or more MSC-derived biological compounds (e.g., growth factors, proteins, etc.).

The invention provides a degradable vaginal repair injection material and application thereof, and relates to the technical field of biomedical materials. The degradable vaginal repair injection material is formed by compounding a dispersing agent and ECM particles, the mass percent of the ECM particles is 20%-80%, and the particle size of the ECM particles is 100-1000 microns. According to the application, the ECM particles are used as a core repairing component, and the ECM particles are rich in bioactive substances such as growth factors and collagen, so that repairing and regeneration of vaginal tissues can be effectively promoted. After being injected into the vagina, the degradable vaginal repair injection material can fill relaxed sites to increase stress after injection, and stimulate tissue collagen regeneration along with the degradation of ECM particles to repair the mechanical properties of the vagina.

The invention relates to a skin wound repairing material and a preparation method thereof, and belongs to the technical field of biological medicines. The repair material disclosed by the invention contains palmitoyl tripeptide-1, methacrylated collagen, cuttlebone extract, recombinant human epidermal growth factor lipidosome, polydopamine nanoparticles, hyaluronic acid, sodium alginate modified silk fibroin and the like in a specific proportion. Wherein the cuttlebone extract is a product which is obtained by carrying out common enzymolysis on cuttlebone through chitinase and nattokinase and then carrying out step-by-step enzymolysis on the cuttlebone through gelatinase and lumbrukinase and is less than 5kDa. The components are prepared into an efficient product by adopting a special method, the stability of the components is improved, the problem that the activity is difficult to maintain is solved, and the skin wound repairing effect is good through reasonable compatibility.