The present application relates to the field of
biochip technology, and specifically to a highly
active polymer, a
biochip, and a preparation method and application thereof. The preparation method of the highly
active polymer comprises: preparing pentafluorophenol
acrylate with
acrylic acid and pentafluorophenol; polymerizing pentafluorophenol
acrylate under the action of an initiator to obtain polypentafluorophenol
acrylate; subjecting polypentafluorophenol acrylate to an amidation reaction with
compound A and compound B to obtain a highly
active polymer; wherein
compound A contains an
azide group and an amino functional group, and compound B contains at least one of an amino group, a carboxyl group, an
epoxy group or a cyano functional group. The present application prepares polypentafluorophenol acrylate by homopolymerizing pentafluorophenol acrylate, using polypentafluorophenol acrylate as a
polymer platform, and introducing the functional group into the
polymer structure through an amidation reaction with
organic matter containing different functional groups. The synthesis process is simple and the preparation cost is low.