The invention discloses a microalgae
polyculture method for adjusting supply of an organic
carbon source through dissolved
oxygen content feedback and application, and belongs to the technical field of microalgae culture, the method comprises the following steps: an autotrophic stage: introducing air into a culture reactor, and enabling cells to reach a certain concentration through autotrophic growth; in the
polyculture stage, ventilation is stopped, dissolved
oxygen is monitored in real time, starting and stopping of an organic
carbon source pump are dynamically adjusted, and mixed
nutrition is achieved; and
continuous production: setting a
dilution rate, continuously harvesting, supplementing the culture medium, and continuously operating. The addition of an organic
carbon source is accurately controlled through the concentration feedback of dissolved
oxygen, the photosynthetic and heterotrophic
metabolism rates are matched, no net oxygen production (stable DO) is ensured, oxygen self-balance in the
system is realized, and finally, the conversion rate of carbon is greatly increased and can reach 0.85 or above. According to the method, the microalgae productivity is remarkably improved under the condition of no
gas exchange, and a new thought is provided for large-scale culture with low
energy consumption and
high carbon efficiency.