The invention discloses a method of producing a 
protein feed by a liquid-
solid two-step 
fermentation method. The method comprises the following steps: performing 
liquid culture on 
corynebacterium glutamicum, candida utilis, 
bacillus subtilis, 
lactobacillus plantarum and 
aspergillus oryzae so as to obtain 
corynebacterium glutamicum 
fermentation liquor, candida utilis 
fermentation liquor, 
bacillus subtilis fermentation liquor, 
lactobacillus plantarum fermentation liquor and 
aspergillus oryzae fermentation liquor; mixing the 
corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the 
bacillus subtilis fermentation liquor, the 
lactobacillus plantarum fermentation liquor and the 
aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of 
soybean meal, cluster bean 
meal, 
cottonseed meal and the like into an explosion vat for performing 
steam explosion; after 
steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an 
enzyme preparation so as to obtain 
mixed materials; conveying the 
mixed materials into a special modularized 
solid-state biological 
raw material continuous fermentation device for enzymolysis and fermentation, and 
drying the fermented materials so as to obtain the 
protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the 
enzyme preparation are mixed, then the 
mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic 
temperature control, automatic dampness control and automatic 
ventilation control are met, the 
bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.