406 results about "Aspergillus" patented technology

The invention relates to the field of microorganisms, and discloses an aspergillus cristatus strain and application thereof. The preservation number of the aspergillus cristatus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41727. The aspergillus cristatus provided by the invention can produce chymosin at high yield, has excellent curding activity and relatively low proteolytic activity, and can reduce the bitter taste of dairy products and improve the yield; the aspergillus cristatus provided by the invention not only can shorten the fermentation time of the dairy product, improve the cream flavor of the dairy product, reduce the peculiar smell of the dairy product, balance the sourness and sweetness of the dairy product and improve the cinnamon flavor, but also has intestinal colonization ability, and has the potential of improving the flavor level of the dairy product and becoming intestinal probiotics.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention discloses a plant-microorganism combined uranium pollution treatment method which comprises the following steps: planting seeds of disinfected uranium-tolerant plants into soil to be remediated, and applying a bacterial suspension of uranium-tolerant strains, namely rhodotorula M1, aspergillus M2 and bacillus M3, into the soil to be remediated, so that the uranium enrichment capacity of the uranium-tolerant plants is remarkably improved; and a relatively good effect of repairing the uranium-polluted soil in the medium-temperature climate region is achieved. Uranium-tolerant plants can be harvested manually or mechanically and transferred to a safe place in a centralized manner to be treated, so that uranium-contaminated soil in the medium-temperature climate zone is effectively repaired.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses a new application of aspergillus flavus in prevention and treatment of epidemic blight of Eupin, and relates to the technical field of microbial prevention and treatment, the aspergillus flavus is CZB-13, the taxonomic name of the aspergillus flavus is Aspergillus flavus, the strain is preserved in China Center for Type Culture Collection on July 8, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20241494. The aspergillus flavus CZB-13 provided by the invention has a remarkable inhibition effect on the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight. The method is also of great significance in protecting the ecological environment and promoting green prevention and control of epidemic diseases of the elaeagnus plants.

The invention discloses application of brevibacillus laterosporus in degradation of ochratoxin A, and belongs to the technical field of microbiology and biodegradation. The preservation number of the brevibacillus laterosporus is CCTCC NO: M 2022932, the brevibacillus laterosporus can degrade the ochratoxin A and prepare a product for degrading the ochratoxin A, the brevibacillus laterosporus participates in degradation of the ochratoxin A in the form of viable bacteria or fermentation liquor, and the degradation rate of the brevibacillus laterosporus to the ochratoxin A is as high as 98.26%. Besides, tests prove that the brevibacillus laterosporus has an antagonistic effect on the aspergillus ochraceus and can be used for preparing an antibacterial product of the aspergillus ochraceus, so that the ochratoxin A is treated from the source.

The invention provides a soy sauce aspergillus HSCY6012 which has high yield of glutaminase, neutral protease and saccharifying enzyme and can reduce the beany flavor of finished koji, the soy sauce aspergillus HSCY6012 is preserved in China General Microbiological Culture Collection Center on April 1, 2024, and the preservation number is CGMCC NO.41133. The invention further provides a preparation method of the soy sauce aspergillus HSCY6012. The soy sauce crude oil prepared by the aspergillus sojae strain HSCY6012 in the soy sauce fermentation method has the advantages that the contents of glutamic acid, reducing sugar and ethanol are increased, the contents of pyroglutamic acid, isoamyl alcohol, isovaleraldehyde and 2-amyl furan are obviously reduced, and the quality of the soy sauce is improved.

The invention provides a conditioner for soil heavy metal pollution remediation and a preparation method thereof, and belongs to the technical field of soil conditioning. The conditioner for soil heavy metal pollution remediation provided by the invention is prepared from the following components in parts by mass: 12 to 25 parts of biochar, 5 to 7 parts of potassium feldspar, 1 to 5 parts of inorganic nano material and 40 to 70 parts of water, the fertilizer is prepared from 40-50 parts of humic acid, 15-25 parts of amino acid, 5-12 parts of citric acid, 4-8 parts of bagasse and 25-40 parts of vinasse. 0.5 to 1 part of aspergillus niger and 0.1 to 1 part of clostridium capsulatum. By utilizing the adsorption of inorganic components on heavy metal elements, the improvement of organic components on saline-alkali soil and the continuous remediation effect of microbial components on the soil, through the synergistic effect of the multiple components, the soil is treated at the same time from multiple aspects of soil fertilization, plant growth, soil loosening, salinity and alkalinity reduction and the like; the soil salinity and alkalinity are reduced, and heavy metals are adsorbed and absorbed.

The invention provides a method for improving the conversion efficiency of fermentation protein for a cyperus esculentus feed and the prepared fermentation protein for the cyperus esculentus feed. Comprising the following steps: uniformly mixing cyperus esculentus cake meal, chitosan oligosaccharide and water, inoculating a fermentation strain, and culturing under proper conditions. Wherein the fermentation strain comprises saccharomycetes or a mixed strain of saccharomycetes and aspergillus niger; the saccharomycetes comprise saccharomyces cerevisiae or a mixed strain of the saccharomyces cerevisiae and candida utilis saccharomycetes. Saccharomyces cerevisiae is used as a main fermentation strain, sugar in fermentation raw materials can be fully utilized, mycoprotein is generated, and the protein content is increased; the candida utilis can utilize various carbon sources and nitrogen sources, so that the protein content and the thallus biomass are further improved; aspergillus niger can generate saccharifying enzyme, starch is hydrolyzed into glucose and other simple saccharides, and the saccharifying process is promoted. According to the method provided by the invention, the cyperus esculentus raw material with relatively low protein content can be converted into the cyperus esculentus feed fermentation protein, and the utilization way of the cyperus esculentus is expanded.

The invention provides a recombinant Aspergillus niger strain with high yield of trans-aconitic acid, and belongs to the technical field of gene engineering. The recombinant aspergillus niger strain can simultaneously express aconitase and aconitic acid isomerase. According to the genetic engineering aspergillus niger strain provided by the invention, the trans-aconitic acid fermentation yield is generally higher than that of an original strain, and the strain with the highest yield can reach 51g / L on a shake flask level, so that the genetic engineering aspergillus niger strain has very high application value.

The invention relates to a technology for shortening the aging time of dried orange peel, and provides a method for improving nobiletin and hesperetin by co-fermenting dried orange peel with two microorganisms, which comprises the following steps: preparing a mixed bacteria solution by using Aspergillus sp. And Exophiala similia; mixing the sterilized pericarpium citri reticulatae powder and the sterilized rice meal to serve as a fermentation substrate, adding the mixed bacterial liquid and the sterilized water into the fermentation substrate to serve as a reaction system, fully stirring the reaction system, and placing the reaction system in a constant-temperature incubator for fermentation. By adopting the method disclosed by the invention, the contents of nobiletin and hesperetin in the dried tangerine or orange peel can be efficiently increased within a relatively short time, the aging time of the dried tangerine or orange peel is shortened, and the conversion efficiency of medicinal effect substances in the dried tangerine or orange peel is improved.

The invention discloses a biocontrol bacterium for preventing and treating soybean phomopsis longicolla caused by the phomopsis longicolla and application of the biocontrol bacterium, the biocontrol bacterium is named as Aspergillus qilianyuensis JSNL-ZJ66, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.41157. The invention further discloses a preparation method of the biocontrol bacterium for preventing and treating the soybean phomopsis longicolla caused by the phomopsis longicolla. The strain is mainly applied to prevention and control of soybean pith seed rot caused by P.lon gilcola, the severity of the soybean pith seed rot can be remarkably reduced, and growth indexes such as the budding rate, the plant height and the fresh weight of the soybean pith seed rot are remarkably improved.

The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention provides a microbial agent capable of degrading lignin and application of the microbial agent in regulation and control of a soil organic carbon library, and relates to the technical field of microorganisms, the microbial agent contains at least two of serratia marcescens, ochrobactrum anthropi, pseudomonas asparagi or burkholderia terricola, and the microbial agent contains at least two of the serratia marcescens, the ochrobactrum anthropi, the pseudomonas asparagi and the burkholderia terricola. The serratia marcescens a26, the ochrobactrum anthropi b1, the pseudomonas asparagi b6 or the burkholderia terricola b21 have good lignin degradation capability, after compounding, the lignin degradation capability of the strain is further improved, and the strain can be used for degrading moso bamboo litters with high lignin content and promoting accumulation of a soil organic carbon library. The technical problem that a microbial agent with a function of efficiently degrading lignin is lacked in the prior art is solved.

The present invention provides antimicrobial compositions comprising an Aspergillus fermentate and methods of making said antimicrobial compositions. Also provided are products comprising the antimicrobial compositions and methods of using the antimicrobial compositions to prevent the growth of a microbe.

The invention relates to the technical field of daily chemicals, in particular to a cacumen biotae fermentation filtrate, a caviar and cacumen biotae nano-coated slow-release composition, shampoo and application. The fermentation filtrate is prepared from cacumen biotae through a liquid fermentation method; wherein the used strain is bacillus and / or aspergillus. The cacumen biotae fermented filtrate provided by the invention contains flavonoid compounds (such as quercetin) and volatile oil (such as cacumen biotae ketone) which can improve scalp blood circulation, nourish hair follicles and promote healthy growth of hair. The shampoo can inhibit hair follicle inflammation and prevent hair follicle atrophy, thereby reducing alopecia. Tannin components can balance grease secretion of scalp, and hair follicle blockage caused by excessive grease is avoided. The shampoo is particularly friendly to oily scalp and is beneficial to keeping the scalp fresh and cool. Polysaccharide and flavonoid substances in the cacumen biotae can prolong the hair growth period and stimulate the activity of hair follicles, so that new hair regeneration is promoted. The plant extract mildly stimulates scalp and does not damage hair follicle tissues.

The present application relates to the technical field of detection of the activity of koji worms, and specifically to a method for judging the activity of koji worms in Daqu based on the content of 1-pentadecene, comprising the following steps: (1) weighing a Daqu sample, adding an extraction solvent to the Daqu sample for leaching, and obtaining an extract; sending the extract to a gas chromatograph-mass spectrometer to detect the content of 1-pentadecene; (2) judging the activity of koji worms in Daqu based on the content of 1-pentadecene detected in step (1). The present application can more accurately judge the activity of koji worms in Daqu samples, and can significantly shorten the time for detecting the activity of koji worms in Daqu samples.

The invention provides a hydroponic symbiotic microbial system and a hydroponic symbiotic method, and relates to the technical field of microbiology. The hydroponic symbiotic microbial system comprises an arbuscular mycorrhizal fungus community, broom cladosporium, aspergillus, pseudomonas and blastomonas. According to the method, a microbial structure in a natural environment is simulated by constructing diversified microbial communities, so that the synergistic effect of the microbial communities is fully exerted; through interaction of modes of co-decomposing organic matters, promoting nutrient absorption, promoting biological control, enhancing plant stress resistance and the like, the stability of an ecological system is co-maintained, and the growth of plants is promoted.

The invention relates to the discovery of a biosynthetic gene cluster of an alkaloid, namely, exopoid C, and the biosynthetic gene cluster of the exopoid. A biosynthetic gene cluster exo of an exopoid compound is obtained from a fungus Penicillium sp.HDN14-431 through genome mining, the gene cluster comprises a heme-dependent oxidase gene exoA which is composed of indole dioxygenase and a cytochrome b5 structural domain, a cytochrome P450 enzyme gene exoB and an isopentenyl transferase gene exoC which is composed of indole dioxygenase and a cytochrome b5 structural domain, and the cytochrome P450 enzyme gene exoB and the isopentenyl transferase gene exoC are used as biosynthetic genes of the exopoid compound. All genes in a gene cluster are expressed through an aspergillus nidulans heterologous expression system, two isopentenyl indole alkaloids are obtained in total, and a compound I is a new compound exopoid C. And the compound II, namely the exopoid, is a known compound. The biosynthesis pathway of the compound is reported for the first time.

Provided is a transfersome cosmetic composition including: a Matricaria flower extract, a Centella asiatica extract, an Arnica montana flower extract, a Scutellaria baicalensis extract, a Paeonia suffruticosa root extract, an aloe vera leaf extract, a Saccharina japonica extract, a licorice root extract, a lactobacillus lysate formed by fermenting strains belonging the lactobacillus genus, a rice bran fermentation filtrate formed by fermenting rice bran using a first composite strain including strains belonging to the bacillus genus, strains belonging to the lactobacillus genus, and yeast, a green tea fermentation extract formed by fermenting green tea using a second composite strain including strains belonging to the Aspergillus genus and strains belonging to the Lactobacillus genus, a grape seed oil, an jojoba seed oil, hydroxyacetophenone, Bifidobacterium, arginine, betaine, trehalose, sodium hyaluronate, hydrolyzed elastin, Ceramide NP, resveratrol, Genistein, tocopheryl acetate, glycerin, and purified water.

The invention discloses a method for generating rare ginsenoside Rh2, which comprises the following steps: adding common glycol type ginsenoside into aspergillus brazilis fermentation liquor for fermentation, and sterilizing after fermentation to obtain a fermentation product; and carrying out enzymolysis by using beta-glucanase and pectinase, and carrying out enzyme deactivation after the enzymolysis is finished, so as to obtain the rare ginsenoside Rh2. The invention also provides the rare ginsenoside Rh2 prepared by the method and application of the rare ginsenoside Rh2. The common glycol type ginsenoside is efficiently and quickly converted into the rare ginsenoside Rh2 through the synergistic interaction of the aspergillus brazilis, the beta-glucanase and the pectinase; the rare ginsenoside Rh2 has the advantages of being simple in preparation method, easy to implement, low in cost, environmentally friendly, free of pollution and the like, and the generated rare ginsenoside Rh2 shows excellent skin care and repairing effects and has wide market prospects when applied to cosmetics.

The present invention discloses a γ-arylbutenolide compound, a preparation method thereof, and an application thereof in the preparation of cardiovascular protective drugs. The above-mentioned structurally novel γ-arylbutenolide compound is isolated for the first time from the fermentation culture of the marine herbal soft coral symbiotic fungus Aspergillus sp. EGF7-0-1, and it is found for the first time that the above-mentioned compound has a protective effect on the cardiovascular system, providing a pharmacodynamic substance basis for the subsequent research on cardiovascular diseases.

To provide a novel technique which enables efficient production of mycelium in a short time.SOLUTION: The present invention provides a method for producing mycelium of koji mold, the method including a culture step involving liquid-culture of koji mold in a culture medium containing grains, and a recovery step involving recovery of the mycelium obtained in the culturing step.SELECTED DRAWING: None

The invention provides an aspergillus terreus JM-F5-6 strain and application thereof in promoting tomato growth and improving soil nutrients, and belongs to the technical field of microorganisms. The preservation number of the aspergillus terreus JM-F5-6 is CCTCC (China Center For Type Culture Collection) NO: M20232616. The conidia of the aspergillus terreus JM-F5-6 are spherical or oval, the surface is smooth, hyphae are colorless or slightly colored, the wall is thick, the hyphae are separated, and hypha branches are laterally connected to form a mesh topology. The aspergillus terreus JM-F5-6 has high protein decomposition capacity and acid production capacity, is beneficial to improving the soil environment, can enhance the photosynthesis and nitrogen absorption of plants and improve the health and growth situation of the plants, effectively improves the content of soil hydrolytic nitrogen, available phosphorus, quick-acting potassium and organic matter, improves the soil fertility, and has wide market application prospects.

The invention discloses a pyranopyridone alkaloid compound as well as a preparation method and application thereof, and belongs to the technical field of medicines. The pyranopyridone alkaloid compound with the activity of resisting the acetaminophen-induced acute liver injury is extracted from a fermentation product of a marine fungus Aspergillus aculeatusWHUF0198, and the pyranopyridone alkaloid compound improves the cell survival rate of mouse hepatocytes treated by acetaminophen and reduces the glutamic-pyruvic transaminase level in a cell supernatant. The pyranopyridone alkaloid compound has the advantages that the pyranopyridone alkaloid compound has the activity of resisting the acetaminophen-induced acute liver injury, and the pyranopyridone alkaloid compound has the activity of resisting the acetaminophen-induced acute liver injury; the pyran pyridone alkaloid compound disclosed by the invention can be used for preparing a medicine for treating acute liver injury. Aiming at the current situation that the types of medicines for treating acute liver injury caused by acetaminophen are deficient at present, the traditional Chinese medicine composition is rich in potential economic benefit.

The invention provides a method for deeply degrading penicillin V through combined circulating cascade of paracoccus and aspergillus niger and application of the method, and belongs to the technical field of antibiotic pollutant degradation, paracoccus is paracoccus sp KDSPL-02, aspergillus niger is Aspergillus niger, and the method is used for deeply mineralizing and degrading penicillin V to dissolve possible pollution caused by the paracoccus sp KDSPL-02 and the aspergillus niger is Aspergillus niger. Waste hyphae generated in the production process are subjected to harmless treatment.

The invention provides aspergillus scherweri for high-yield cellulase and application of the aspergillus scherweri in fermentation of Chinese herbal medicines. The Aspergillus scherweri J-10 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Aspergillus scherweri J-10 is CGMCC No. 41630. The Aspergillus scherweri J-10 disclosed by the invention has the advantages that the Aspergillus scherweri J-10 can be used for preparing the Aspergillus When the aspergillus scherweri provided by the invention is used for fermenting Chinese herbal medicines represented by lucid ganoderma, the release and utilization rate of active ingredients of the Chinese herbal medicines can be remarkably improved. An in-vitro anti-tumor activity result shows that the alcohol extract of the Chinese herbal medicine (represented by ganoderma lucidum sporocarp) fermented by the J-10 strain has a stronger in-vitro inhibition effect on various tumor cells, and a new method and way are provided for deep processing and comprehensive utilization of the Chinese herbal medicine.

The invention provides plant fermented oil with moistening and soothing effects and a preparation method thereof. The preparation method comprises the following steps: fermenting aspergillus in a fermentation culture medium 1, adding candida albicans, inoculating into a fermentation culture medium 2, carrying out primary fermentation under the ventilation capacity of 2-4 L / min, carrying out secondary fermentation under the ventilation capacity of 0.2-0.5 L / min, separating, and sterilizing to obtain the plant fermented oil. The aspergillus and candida mellifera are mixed and fermented according to a specific sequence, so that the obtained plant fermented oil has excellent soothing and moistening effects. Besides, compared with products obtained through fermentation in other modes, the plant fermentation oil has more excellent soothing and moistening effects and is very suitable for being used as and / or used for preparing cosmetics or medical compositions.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.