135 results about "Aspergillus" patented technology

The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

This application relates to a terphenylamine skeleton compound, its preparation method, and its application, belonging to the field of chemical synthesis technology. The preparation method of the terphenylamine skeleton compound of this application includes the following steps: dissolving 2,4,6-triphenylaniline with thiazole-4-carboxaldehyde or thiophene-3-carboxaldehyde in a solvent, conducting a reductive amination reaction under reducing agent conditions, quenching, extraction, washing, concentration to remove solvent, and purification to obtain the terphenylamine skeleton compound. This application provides a simple and mild synthetic method for the natural active products 5'-phenyl-N-(thiazol-4-ylmethyl)-[1,1':3',1''-terphenyl]-2'-amine and 5'-phenyl-N-(thiophene-3-ylmethyl)-[1,1':3',1''-terphenyl]-2'-amine, which can only be extracted from the secondary metabolites of the marine fungus Aspergillus sp. Furthermore, this method utilizes widely available raw materials, which is beneficial for large-scale industrial production of these natural active products.

The application belongs to the technical field of microbial fermentation, and particularly relates to a kind of cytolysis product of mixed bacteria fermentation and a preparation method. The cytolysis product is prepared by mixed bacteria fermentation of two kinds of aerobic probiotics, i.e. Lactobacillus plantarum and Aspergillus oryzae are used as fermentation strains to carry out mixed bacteria fermentation treatment on rice milk, and membrane filtration and column chromatography system are used for filtration and enrichment, so as to obtain a cytolysis fermentation product filtrate rich in kojic acid, the content of kojic acid is greater than 200 g / L, which is significantly improved compared with conventional process. The obtained cytolysis product has no bad smell and clear color, so that the beneficial function of kojic acid can be played only with a low addition amount in cosmetics, and the addition of odor masking and color correcting auxiliaries is reduced, and the cytolysis product can be used for preparing cosmetics with skin repair, whitening and other functions.

The application discloses a kind of fermentation complex microbial inoculant and preparation method and application thereof, the fermentation complex microbial inoculant includes the following weight parts of component: bacillus subtilis microbial inoculant 2-5 parts, bacillus mucilaginosus microbial inoculant 5-10 parts, yeast microbial inoculant 2-10 parts, trichoderma harzianum microbial inoculant 10-20 parts, aspergillus niger microbial inoculant 10-20 parts and streptococcus thermophilus microbial inoculant 2-5 parts;The fermentation complex microbial inoculant of the application is prepared by selecting each strain and amount, is added to pigeon manure and is fermented to prepare organic fertilizer, can better decompose macromolecular substance, significantly improve the organic matter and total nitrogen content in preparation organic fertilizer, and can better improve seed germination rate;In addition, the preparation method of pigeon manure organic fertilizer of the application is simple in process, easy to operate, and convenient for production.

The invention discloses a novel functional microbiome fortified yeast and application thereof in improving the quality of Shanxi mature vinegar. The invention provides a combined bacterium, which is prepared from Aspergillus sp. (ACCC 34193), Saccharomycopsis fibulligera (9203) ACCC 21511, Lichtheimia ramosa (5977) ACCC 34192, Bacillus velezensis (9386) ACCC 64506, and Lactobacillus plantarum (9600) ACCC 64531, and the combined bacterium is prepared from the following raw materials: Aspergillus sp. (9177), Saccharomycopsis fibulligera (9203), ACCC 21511, Lichtheimia ramosa (5977), ACCC 64506, and Lactobacillus plantarum (9600). The invention also provides a fortified yeast containing the combined bacteria. Compared with the traditional vinegar koji, the Shanxi mature vinegar prepared by using the strengthened koji disclosed by the invention not only improves the saccharification power, flavor and appearance of the vinegar koji, but also effectively improves the quality, flavor and functional substance content of the mature vinegar.

The present invention relates to the technical field of clinical medicine immunodetection, and discloses an aspergillus galactomannan detection kit and a preparation method thereof, the aspergillus galactomannan detection kit comprises a test paper card and a sample treatment liquid, the test paper card comprises a bottom plate, and a sample pad, a labeling pad A, a labeling pad B, a nitrocellulose membrane and water absorption paper which are sequentially arranged from a sample adding end on the surface of the bottom plate, the labeling pad A is provided with a quantum dot-biotin labeled aspergillus galactomannan monoclonal antibody, the labeling pad B is provided with microspheres coupled with the aspergillus galactomannan monoclonal antibody, and the nitrocellulose membrane is coated with streptavidin and goat anti-mouse IgG. According to the invention, the defect of low sensitivity can be overcome.

The application provides a composite microbial agent, application of the composite microbial agent and a treatment method of high-concentration organic wastewater. The active component of the composite microbial agent provided in the first aspect of the application comprises two or three of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain. The composite microbial agent provided in the first aspect of the application can be used for biological decontamination treatment of high-concentration organic wastewater, so as to realize degradation of organic pollutants in the high-concentration organic wastewater and pH value adjustment, thereby avoiding increase of the load of a municipal sewage treatment system.

The application relates to the technical field of semiconductor nanomaterials, and discloses an environment-friendly zinc sulfide nanoparticle as well as a preparation method and application of the zinc sulfide nanoparticle. The preparation method comprises the following steps: (1) activating Aspergillus oryzae spores, performing liquid fermentation, filtering the liquid fermentation product, washing, collecting solid substances, and obtaining biomass; (2) culturing the biomass in the step (1) in a ZnSO4.7H2O aqueous solution to obtain an Aspergillus oryzae liquid solution containing zinc sulfide nanoparticles; (3) drying the liquid solution obtained in the step (2) to obtain Aspergillus oryzae precipitate containing zinc sulfide nanoparticles; and (4) heating the precipitate in the step (3) at 400-450 DEG C for 4-6 h to obtain zinc sulfide nanoparticles. The application endows the zinc sulfide nanoparticles with excellent biocompatibility, expands the application of the zinc sulfide nanoparticles in the fields of biomedicine, chemical sensors, solar cells and light-emitting diodes, and provides a reference for the environment-friendly and batch production of the zinc sulfide nanoparticles.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The present application relates to a kind of mung bean skin fermentation and its preparation method and application of reinforced anti-oxidation, anti-aging effect, belong to the field of microbial technology.The present application provides a kind of preparation method of mung bean skin fermentation of reinforced anti-oxidation, anti-aging effect, including the following steps: after being mixed with white aspergillus, mung bean skin containing solid-state fermentation medium is carried out aerobic fermentation, after fermentation is ended, it is sterilized and is treated, and the pretreated mung bean skin is obtained;Bifidobacterium is inoculated into the culture medium containing pretreated mung bean skin anaerobic fermentation, and mung bean skin fermentation is obtained.The present application is by mung bean skin as one of the main components of solid-state fermentation medium and white aspergillus aerobic fermentation, so that the cell wall of mung bean skin is degraded, and reducing sugar and polyphenol are released, are used by bifidobacterium, so that the fermentation efficiency of bifidobacterium is improved, and then the content of active ingredient in mung bean skin fermentation is improved, and mung bean skin fermentation is endowed with excellent anti-oxidation, anti-aging effect.

The application discloses application of gynostemma pentaphyllum saponin H combined with azole drugs in preparation of antifungal drugs and the antifungal drugs, and relates to the technical field of antifungal drugs. It is found through systematic in-vitro drug sensitivity test that natural product gynostemma pentaphyllum saponin H combined with specific azole antifungal drugs (especially posaconazole POS) can produce significant synergistic antifungal effect, especially for clinical thorny aspergillus, candida, new cryptococcus and dark fungi. The combination strategy can significantly reduce the minimum inhibitory concentration of azole drugs, reverse drug resistance, and is effective for multi-drug resistant strains (such as candida auris). The application provides an efficient and low-toxicity new combination drug scheme for overcoming the increasingly serious fungal drug resistance problem.

The application discloses a steroid-indole alkaloid hybrid dimer compound, a preparation method thereof and application of the compound in preparation of an antitumor chemotherapy drug sensitizer, and first prepares the steroid-indole diketopiperazine alkaloid hybrid compound from a fungus Aspergillus sp. EGF15-0-3 rice culture medium. The compound is a new skeleton compound first discovered in nature. It is first discovered that the hybrid compound has Tdp1 inhibitory activity and can be applied as a tyrosine-DNA phosphodiesterase 1 (Tdp1) inhibitor. It is first discovered that the hybrid compound has chemotherapy sensitization activity on a tumor chemotherapy drug topotecan TPT, which provides a drug source molecule and data support for development of a new natural antitumor combination inhibitor, and has important significance for development and utilization of marine Aspergillus sp. fungus resources.

The invention belongs to the technical field of plant disease control, and particularly relates to a method for controlling root rot of cardamine violifolia. The technical problem to be solved by the invention is to clarify the pathogenic bacteria of cardamine violifolia and provide a new choice for preventing and treating cardamine violifolia root rot. The invention also provides a method for preventing and treating the root rot of cardamine violifolia. The method comprises the following step: treating the cardamine violifolia by adopting at least one of hymexazol (T1), oxaconazole (T2) and propiconazole (T9). According to the application, the key pathogenic bacteria of the root rot of the Enshi cardamine violifolia are determined to be Aspergillus Goddavidii (A2), Mucor circinelloides Luusitanius variant (A3) and Blumeria tuberosa (A6) for the first time. According to the invention, bactericides, namely propiconazole, hymexazol and oxadiazole, which have a remarkable inhibition effect on three pathogenic bacteria are screened from nine conventional bactericides, and the mixed use effect of propiconazole, hymexazol and oxadiazole is better; a new scheme is provided for preventing and treating the root rot of cardamine violifolia.

The invention discloses low-caffeine anti-aging fermented tea and a preparation method thereof, and belongs to the technical field of tea processing. According to the invention, an Aspergillus Tennesensis strain is obtained by screening, and the preservation number of the Aspergillus Tennesensis strain is CGMCC (China General Microbiological Culture Collection Center) No.42415. The method comprises the following steps: inoculating aspergillus Tennesensis into a culture medium only containing tea leaves and water, fermenting, and airing after aspergillus Tennesensis hyphae completely cover the tea leaves, so as to obtain the low-caffeine anti-aging fermented tea. The aspergillus Tennesensis is used for degrading caffeine in the tea leaves, the growth speed is high, the degradation efficiency is high, and the degradation rate of the caffeine can reach 98% or above after 20 days of fermentation; the fermented tea leaves have very excellent antioxidant activity. The method for preparing the fermented tea is high in operability, the prepared fermented tea is red and dark in tea soup, black brown, mellow, smooth and low in bitterness degree, and the flavor quality of the tea leaves is improved.

This application relates to a novel Aspergillus neoniger, biological agents, tobacco products, and methods for preparing the same. The novel Aspergillus neoniger has the accession number CGMCC No. 42429. This strain is used to selectively improve the flavor of cigar tobacco leaves during fermentation, effectively transferring the "mellow, sweet, and pronounced aftertaste" qualities of dark tea to the tobacco product. This significantly enhances the sweetness, smoothness, and aftertaste quality of the fermented cigar tobacco leaves while reducing their harshness.

Methods and kits are described for testing for the presence or absence of any fungus in a sample. Examples of fungi that can be detected include, but are not limited to, those belonging to the genera Candida, Aspergillus and Pneumocystis. The methods include obtaining a sample suspected of containing fungal nucleic acid, including at least one universal region of fungal nucleic acid, and testing for the presence or absence in the sample of the at least one universal region of fungal nucleic acid. Samples may be biological or non-biological.

Solid frecze-dried animal food compositions and methods of preparing and using the animal food compositions are disclosed. The animal food compositions comprise a yeast product. an Aspergillus sp. product, and essential nutrients.

The application discloses a kind of biological inoculums for improving fermentation environment of waste pool of waste incineration plant, solve high kitchen garbage heavy metal pollution problem.It uses "functional strain + kitchen waste oil modified biochar carrier" collaborative design, functional strain includes Yarrowia lipolytica, Aspergillus oryzae, salt-tolerant lactic acid bacteria and Rhodotorula, Rhodotorula can efficiently passivate Pb 2+ , Cd 2+ , strain synergistic degradation of oil and starch.Application of percolate soluble heavy metal content reaches standard, reduces heavy metal treatment reagent dosage 25%-30%, reduces fly ash "hazardous waste" disposal ratio, realizes fermentation improvement and heavy metal passivation integration, with environmental protection and economic benefits.

This invention provides a fermentation method to enhance the aged and woody aroma of Fu brick tea, belonging to the field of Fu brick tea processing technology. Based on *Aspergillus chevaleri*, a strain deposited at the China General Microbiological Culture Collection Center (CGMCC No. 42256), the method involves adding plant-derived methyl selenide, such as selenium-enriched freeze-dried rapeseed powder, to black tea to adjust the total selenium content. After inoculation, fermentation is carried out, followed by drying. The resulting Fu brick tea exhibits significantly increased levels of characteristic aged and woody aroma substances, such as 4-ethylphenol and 1,2,3-methoxybenzene, exhibiting a mellow and robust aged and woody aroma. This invention uses plant-derived methyl selenide instead of chemical selenium additives, eliminating the need to change the fermentation strain and process parameters. The intensity of the aged and woody aroma can be precisely controlled simply by adjusting the amount of naturally selenium-enriched raw materials. The process is simple, controllable, and the product is natural and safe, fully meeting the food safety requirements for tea, making it highly suitable for large-scale industrial production.

The present application belongs to the technical field of water pollution treatment, and relates to microbial treatment, in particular to an aged iron filings promoted aerobic iron-reducing fungi for denitrification and application thereof. Aspergillus The aerobic iron-reducing fungi is classified and named as sp. R4, and is preserved in China Center for Type Culture Collection on February 13, 2025, with a preservation number of CCTCC NO: M 2025217. Aquabacter cavernae 1-5 are products obtained by iron filings oxidation in the process of denitrification. The present application provides a new insight for the repair of low carbon-nitrogen ratio water body.

The invention relates to Aspergillus oryzae MY. The Aspergillus oryzae MY can rapidly and stably grow by using a cheap culture medium, the fermentation period is short, and the degradation efficiency of lignin and cellulose is high. The strain can efficiently degrade straws, and can be used as a substrate for producing alternative proteins for feed; and reducing sugar can be produced by degrading cellulose, so that the method can be industrially applied and has very high economic value and ecological value.

The invention discloses a fermentation process and application of citric acid waste residues, and belongs to the technical field of biotech, the fermentation process comprises the following steps: S1, uniformly mixing citric acid waste residue powder, wheat bran and rice hull powder balls, performing high-pressure steam sterilization, adding sterile water, a urea solution and a basic nutrient solution, and uniformly stirring to obtain a fermentation substrate; s2, the fermentation substrate is placed in a fermentation tank, aspergillus niger liquid is inoculated, fermentation culture is conducted for 60-96 h, then candida utilis yeast liquid is inoculated, fermentation culture is conducted for 36-72 h, finally, lactobacillus plantarum liquid is inoculated, fermentation culture is conducted for 48-72 h, and the fermentation process is completed. Time sequence regulation and control are performed on flora metabolism, and finally the goals that the crude protein content of a fermentation product is obviously increased and the crude fiber content is effectively reduced are achieved.

The present application provides a mildew inhibitor and its application in tobacco recombination raw materials. The mildew inhibitor mainly comprises clove compound essential oil composed of clove bud essential oil, star anise essential oil and cassia essential oil, and the mass ratio of the clove bud essential oil, the star anise essential oil and the cassia essential oil is 10:(0-4):(0-6). Through tests, the clove compound essential oil can inhibit the growth of various molds such as Aspergillus flavus, Aspergillus niger and Aspergillus montgenieri. The mildew inhibitor has a broad-spectrum inhibitory effect on common Aspergillus of tobacco leaves, can be used as a tobacco mildew inhibitor, can be used for preventing and treating the mildew of tobacco recombination raw materials, and can be in harmony with the tobacco aroma of heated cigarette smoke, so that the mildew inhibition and aroma enhancement effects on the tobacco recombination raw materials are achieved. In addition, as a tobacco mildew prevention additive, the application method of the mildew inhibitor is simple, diverse and adjustable, the integration degree with the core material preparation process is high, and the mold growth inhibition effect is obvious.

The application discloses an indole dione piperazine alkaloid compound and a preparation method and application thereof, and belongs to the technical field of marine natural products. The Aspergillus sp. SYPHU504 is preserved in the Guangdong Microbial Culture Collection Center on August 26, 2024, and the preservation number is GDMCC No: 65055. The indole dione piperazine alkaloid compound and a pharmaceutically acceptable salt, isomer or solvate thereof are prepared from a fermentation culture of the Aspergillus sp. SYPHU504. It is found through experiments that the indole dione piperazine alkaloid compound can significantly inhibit the proliferation of leukemia cell strains K562 and RS4:11 in a dose-dependent manner. Therefore, the indole dione piperazine alkaloid compound is expected to be developed into a safe and effective novel anti-leukemia drug, and has important significance for the development of Chinese marine drug resources.

The application discloses a kind of cyclopentapeptide compounds and its application in alpha-glycosidase inhibitor hypoglycemic drugs or preventive health products.The application provides a kind of marine fungi, name is Aspergillus sp.TW58-5, preservation number: GDMCC No:62093.The application utilizes the polarity difference of peptide compound to extract, separate and obtain a kind of cyclopentapeptide compound from the fermentation culture of marine fungi, the method is simple in operation, and the yield is high, and the product purity is high, suitable for large-scale production.Through in vitro alpha-glucosidase inhibitory activity evaluation, it shows that cyclopentapeptide compound has better alpha-glucosidase inhibitory activity, and the inhibition rate of alpha-glucosidase is 74%~100% at 400 μM concentration, which is stronger than positive drug acarbose, and can be used for preparing alpha-glucosidase inhibitor hypoglycemic drugs and preventive health food, with good development prospect.

The utility model discloses a yeast turning device for soy sauce aspergillus culture. According to the technical key points, the device comprises a fermentation box used for containing aspergillus, a supporting mechanism used for supporting the fermentation box and a driving mechanism are arranged below the fermentation box, the driving mechanism comprises a motor and a transmission shaft connected with an output shaft of the motor, and a shaft sleeve used for being connected with the transmission shaft in a clamped mode is arranged in the fermentation box; and a stirring piece for stirring aspergillus is arranged on the shaft sleeve. The device has the advantages that the eccentric wheel drives the ejector rod to move upwards, so that the fermentation box generates intermittent vibration, the structure of an aspergillus mass becomes loose, internal heat can be dissipated during fermentation, the aspergillus mass is kept in a temperature environment suitable for fermentation, and the fermentation quality is improved; and the stirring piece can stir the aspergillus at the bottom to the surface, so that the effect of internal and external exchange of the aspergillus is realized, the heat of the internal aspergillus is fully dissipated, and the aspergillus with higher quality is prepared.

The application discloses an Aspergillus oryzae strain WJM008, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 20221829, and the preservation date is November 28, 2022. The application also discloses an application of the Aspergillus oryzae strain WJM008 in flavor soy sauce brewing. The strain has a strong ability of producing non-starch polysaccharase, can well hydrolyze non-starch polysaccharide of raw materials, realizes efficient utilization of raw materials for soy sauce brewing, and provides sufficient carbon source for yeast alcohol fermentation due to that sugar in the raw materials is fully released, so that the original oil has rich and mellow alcohol aroma.

The invention discloses a screening method and application of natural small molecules for inhibiting growth of Aspergillus carbonniger and synthesis of ochratoxin A, and relates to the technical field of bioinformatics. The invention particularly provides an application of coumarin or coumarin derivatives in preparation of a preparation for inhibiting growth of aspergillus carbonus niger and / or synthesis of ochratoxin A. The invention further provides a preparation method of the coumarin or the coumarin derivatives. According to the invention, SE of Aspergillus carbonus is taken as a target spot, small molecules with potential inhibitory activity are rapidly screened from a natural compound library by using a virtual screening technology, and experiments prove that coumarin and specific derivatives thereof not only can effectively inhibit the growth of Aspergillus carbonus, but also can efficiently inhibit the biosynthesis of OTA, so that the coumarin and specific derivatives thereof can be used for preparing drugs for inhibiting OTA. A theoretical basis and technical support are provided for the application of the strain in the postharvest preservation of agricultural products.

The invention relates to a biological agent for degrading bamboo shoot leftovers, a fertilizer and a preparation method and application thereof. The biological agent comprises an organic material decomposition agent, a nitrogen source and a compound enzyme, the organic material decomposition agent comprises paenibacillus mucilaginosus, lactic acid bacteria, saccharomycetes and aspergillus niger, and the compound enzyme comprises xylanase, cellulase, pectinase and alpha-amylase. According to the biological agent disclosed by the invention, the organic material decomposition agent and the compound enzyme are optimized, and the organic material decomposition agent and the compound enzyme with proper composition are compounded, so that the fermentation degradation effect of the bamboo shoot leftovers can be effectively improved. The fertilizer obtained by fermenting and degrading the bamboo shoot leftovers by using the biological agent can effectively promote the growth of the bamboo shoots, obviously improve the yield of the bamboo shoots and effectively reduce the utilization amount of the fertilizer in the production process of the bamboo shoots.