901 results about "Aspergillus" patented technology

The subject invention provides for the production of lactoferrins and lactoferrin polypeptide fragments using the host cells Aspergillus in combination with novel plasmid constructs. More specifically, the subject invention provides novel vector constructs capable of producing lactoferrins and lactoferrin polypeptide fragments in Aspergillus host cells. More particularly, the subject invention provides for novel plasmid constructs suitable for use with Aspergillus and especially Aspergillus awamori, niger and oryzae host cells, which enables them to produce large amounts of recombinant lactoferrins and lactoferrin polypeptide fragments.

The invention discloses a composite efficient microbial preparation for treating difficultly-degradable waste water. The composite efficient microbial preparation comprises the following active ingredients of: bacillus, pseudomonas, alcaligenes eutrophus, aspergillus and yeast. The composite efficient microbial preparation has a unique treating effect on high-concentration organic sewage and highammonia and nitrogen sewage which are difficult to treat by the conventional activated sludge process and contain macromolecules and difficultly-degradable harmful constituents.

A microbial leaven used for preparing organic or microbial fertilizer from organic waste is prepared from at least two of nine microbes. Aspergillus, Sporotrichun, Mucoraceae, Penicillium sp, Ceotrichum sp., Neurospora, Bacillus, Saccharomyces and Streptomyces sp.

The invention provides a method for preparing a biological feed by taking manioc residues as raw materials. The method comprises the following steps of: preparing fermentation strains and fermentation mother cultures from aspergillus, bacillaceae, lievito and lactobacillus, and performing mixed fermentation by taking the manioc residues as the raw materials to obtain a biological feed crude product; and mixing uniformly, crushing, sieving, drying and packaging to obtain a biological feed finished product. According to the method, the manioc residues are used as the raw materials, so the biological feed is low in cost and wide in source, industrial waste residues are recycled, and the problem of the pollution of the manioc residues is solved. Simultaneously, the strains which are high in cellulose degradation effect and high in yield of mycoprotein are selected, so that cellulose in the manioc residues is degraded fully, the protein content of the feed is improved, a large number of enzymes, beneficial viable bacteria and organic acids are added.

The invention relates to preparation methods and uses of two dioxypiperazine compounds. The invention adopts aspergillus effuses H1-1 from root mud of Fujian mangrove forest to product novel structural dioxypiperazine compounds. Tests prove that: the compounds can be used as cellular breeding depressant or anti-tumor agent.

Object: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.Method for Achieving the Object: A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

A slow-release solid chemical composition for environmental bioremediation is provided. The composition comprises a source of soluble organic substrates which include sugars, soluble organic polymers and mixtures of them in an amount of 7% to 90%, insoluble organic substrates an amount of 10% to 70%, complex inorganic phosphates in an amount of 0.5% to 7% and soluble organic salts in an amount of 2% to 70%. The insoluble organic substrates include fibrous plant materials, starches, cellulosic materials and mixtures of these substrates. The complex inorganic phosphates include ringed metaphosphates, linear polyphosphates and mixtures. The organic salts include lactates, formates, acetates, citrates, etc. Also the composition further comprises microorganisms which include Bacillus spp., Rhizobium spp., Bradyrhibzobium spp., Fibrobacter spp., Clostridium spp., Pseudomonas. spp., Geobacter spp., Arthrobacter spp., Nocardia, spp., aspergillus spp., Trichoderma spp., Candida spp., Yarrowia spp. and combinations of these microorganisms. The composition can be prepared in various forms, including granules, briquettes, pellets, tablets or capsules.

The invention provides a biological organic fertilizer prepared by aerobic fermentation of a biogas residue and a preparation method thereof. The preparation method comprises the steps: mixing the biogas residue and a swelling agent to form a fermentation raw material, inoculating a compound microbial agent in the fermentation raw material, uniformly mixing for fermenting, and adding urea, diammonium phosphate, a potassium chloride inorganic salt, brown nitrogen-fixing bacteria, mycorrhiza fungi, silicate bacteria, photosynthetic bacteria acetic bacteria, bifidobacterium, and saccharomycetes after the fermentation is completed to prepare the biological organic fertilizer, wherein the compound microbial agent comprises strains of bacillus, pseudomonas, staphylococcus, streptomyces, penicillium, aspergillus and trichoderma. The compound microbial agent prepared by using the biological organic fertilizer has a pertinence to aerobic fermentation of the biogas residue, and is capable of effectively increasing the fermentation rate of the biogas residue, shortening the fermentation time and realizing high-additional value production, innocent treatment and recycling of the biogas residue; and the problem of resources and environment of villages and small towns is solved, and the rapid development of construction of new countryside and towns and cities can be promoted.

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and / or improved thermostability.

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

The invention belongs to the technical field of environmental microorganisms, and especially relates to an organic material decomposing agent and its preparation method. The preparation method comprises the following steps: preparing strains, drying, crushing, sieving, and mixing 35-55 parts of Bacillus spp with 2-5 parts of yeast, 5-10 parts of aspergillus, 4-20 parts of Streptomyces thermophilus, 8-15 parts of Thermoactinomyces sp and 20-30 parts of high temperature cellulose decomposition bacteria. The organic material decomposing agent can utilize easy-degradation components comprising starch, proteins and the like in a compost in order to accelerate the heating of the compost, and can also secrete digestive enzyme to accelerate the decomposition of difficult-decomposition substances comprising pectin, cellulose, lignin and the like without interfering the full performance of the microorganisms, so the composting efficiency and the quality of a composted finished product are improved, and the organic material decomposing agent has the advantages of low price, wide application range, strong practicability and easy popularization.

The present invention relates to one kind of high protein feed produced through mixing and fermenting paper-mulberry leaf and distiller's grains and its production process. The production process includes the following steps: stoving and crushing paper-mulberry leaf into powder; mixing yellow wine residue and water, sterilizing, cooling, adding aspergillus and candidia strain and fermentation culturing to obtain fermenting yeast; mixing paper-mulberry leaf powder, pressed yellow wine residue and water to form solid fermenting material, inoculating the fermenting yeast to ferment; and stoving and crushing or pelletizing the fermented material to produce the feed. The high protein feed product contains protein in 23-45 wt%, carbohydrate in 42-48 wt%, crude fiber in 6-7 % and lysine in 2 wt%, as well as rich vitamin B and beneficial enzymes, and is one excellent kinds of feed for farm animals.

The invention discloses an Aspergillus flavus strain for not producing toxins of Aspergillus flavus, the Aspergillus flavus strain belongs to the Aspergillus flavus of Aspergillus, the collection name is: Aspergillus flavus strain A051, the collection unit is: China General Microbiological Culture Collection Center, the collection data is: June 24, 2008, and the collection No. is: CGMCC NO.2556. The invention further discloses the use of the Aspergillus flavus strain, and the use is characterized in that: the Aspergillus flavus strain is used for inhibiting Aspergillus flavus pathogenic bacteria groups of the toxins of the Aspergillus flavus in peanut field soil.

The invention provides an ecological restoring method of a river water body, comprising the following steps: (1) detecting pollution indexes of a river water body; (2) throwing composite microbial agents into a water body according to 5ppm to 9ppm; (3) transferring, translating and degrading matters in the river water body by the composite microbial agents; (4) throwing and / or planting water plants into a river; (5) detecting the pollution indexes of the river water body; and (6) repeating the steps from (2) to (5) from 15 days to 30 days until the river water body conforms to requirements. Each composite microbial agent comprises the components by weight percent: 5 to 10 photosynthetic bacteria, 10 to 15 bacillus subtilis, 5 to 10 nitrifying bacteria, 2 to 5 microzyme, 15 to 20 lactobacillus, 10 to 30 aspergillus, 10 to 30 acetobacter and 20 to 30 actinomyces. The ecological restoring method of a river water body aims at a river water body which carries out eutrophication treatment, leads the river water body to restore the bio-diversity of the river water body, radically restores ecology and has durable effect, low cost and simple use.

The invention provides a traditional Chinese medicine multi-enzyme bacterium straw composite feed for livestock and poultry. The feed is mainly prepared from saccharomyces cerevisiae, protease, phytase, cellulose, xylanase, glucanase, laccase, pectase, amylase, Aspergillus niger, traditional Chinese medicinal preparations consisting of Radix Astragali, jerusalem artichoke, Poria cocos, purslane, dandelion and the like, straw, corn flour, potassium dihydrogen phosphate and magnesium sulfate magnesium sulfate. According to the invention, a complex enzyme system and microbes exert action on the straw, and synergism between traditional Chinese medicines and potassium dihydrogen phosphate and magnesium sulfate is used at the same time; so output of bifidobacteria in the livestock and poultry is increased, disease-resistant immunity of the livestock and poultry is improved, and bifidobacteria exerts effects on assisting digestion and decomposing plant barriers in coarse cereals so as to realize easy absorption of the coarse cereals by intestines and stomachs and assist the livestock and poultry in rapid digestion and absorption of foods. Thus, the livestock and poultry like flocks and herds are allowed to have healthy and strong bodies, an enhanced digestive function and an improved farrowing rate, fattening and marketing time is shortened, and breeding cost is reduced.

Systems are provided for preventing post harvest fungal diseases of food systems, such as but not limited to fresh produce, such as but not limited to berries (e.g., blueberries). For example, various anti-fungal compounds can incorporated or encapsulated into cyclodextrins, such as but not limited to α, β and / or γ cyclodextrins. The encapsulated anti-fungal materials can be used alone (e.g., brought into proximity to the produce) or incorporated into film and / or packaging materials that are used in the packing and / or storing of produce. By way of a non-limiting example, the anti-fungal compounds can include volatile compounds such as but not limited to acetaldehyde, hexanal and 2E-hexenal. The cyclodextrins provide controlled release of the volatiles over a period of at least several days such that they prevent or inhibit fungal growth, including but not limited to several species of the Colletotrichum, Altermaria, Botrytis, Penicillium and / or Aspergillus genera.

A fermentation production method after the pile fermentation of cooked Puerh tea is characterized in that the Yunnan large-leaf Puerh tea is taken to make Puerh raw tea after the tea goes through the process of water removing, kneading and drying which is also called the crude green tea; the ''Puerh tea aspergillus'' is added into the crude green tea for pile fermentation, i.e. the crude green tea is spread in a fermentation pond and a proper special bacterium which is the ''Puerh tea aspergillus'' is diluted with fresh water and spread on the tea evenly layer by layer; and fresh water is used to moisten the tea until the water content reaches 35 to 38 percent; the pile fermentation can be implemented after the tea is mixed evenly; the pile height is 1.0 to 1.2 meters and the pH value is controlled between 6.0 to 6.5; the tea tightly covered for fermentation for 40 to 45 days and the fermentation temperature is 30 to 59 Celsius degrees; the fermentation process is monitored dynamically and the pile is turned according to the temperature changes in the pile; and Puerh tea can be obtained after fermentation, drying and aging. The present invention has simple fermentation process. On the basis of the traditional pile fermentation method of Puerh cooked tea, the method adopts special bacterium fro fermentation and the inoculated special bacterium is an advantageous microbe bacteria colony which can effectively control the fermentation process and related factors and shorten the fermentation cycle; in this way, the fermentation success rate is increased and the standard production of Puerh cooked tea is realized and the quality can be ensured to be stable.

The invention, pertaining to a field of food brewing technology, particularly relates to a kidney bean sauce and a brewing method thereof. According to the invention, white kidney beans are used as the main raw material, flour and preserved fruit syrup are used as auxiliary materials, a plurality of strains such as aspergillus oryzae, aspergillus niger, lactic acid bacteria, yeast and the like are used as fermentation microorganisms, and the product is obtained after three stages of temperature-controlled fermentation, that is, flavor-generating fresh fermentation, chromophore-generating fermentation, and aroma-generating fermentation. The method of the invention, by adopting a band temperature-controlled method, controls growth of the aspergillus, maintains high activity and quantity of the protease, carries out enzymolysis on the kidney bean protein, and produces large amounts of amino acid and peptide. By using the lactic acid bacteria and the yeast for fermentation, and an insulation reaction, the final product has unique and rich sauce flavor and color. The sauce of the invention tastes fresh and delicious, has a total ammonia nitrogen content of 1.64% which increases by 26% compared with that of traditional methods, and has abundant volatile flavor substance.

The present invention relates to preparation of Fructooligosaccharide (FOS). The FOS in prepared by the reaction of extracellular Fructosyl Transferase (FTase) enzyme obtained from Aspergillus species.

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) universal premier for detecting aspergillus, detection probes and a detection kit, wherein the detection kit comprises universal premiers displayed by base compositions such as SEQ ID. NO: 1 and SEQ ID. NO: 2; and the detection probe is CY5-TAAAGTTGGGTGTCGGCTGG-BHQ aiming at aspergillus fumigatus, the detection probe is FAM-TTGATTTGCGTTCGGCAAGC-BHQ aiming at aspergillus flavus, the detection probe is HEX-ACAAGTTGCAAATAAATGCGTCG-BHQ aiming at aspergillus, and / or the detection probe is ROX-ATGGTTGGAAAACGTCGGCA-BHQ aiming at aspergillus Niger. The multiple PCR detection method, premier, the probes and the kit thereof provided by the invention aim at four main pathogenic aspergilli and have high specificity and sensitivity; and the detection method is rapid, simple and convenient, and can be used for detecting and identifying the strain of pathogenic bacteria.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention discloses a modifying agent capable of passivating vegetable garden soil heavy metal and a preparation method and an applying method thereof. The modifying agent consists of organic material and potassium polyacrylate in a mass ratio of 7:(3-9):1; the organic material comprises the following components in parts by weight: 100-120 parts of gramineous crop straws, 10-12 parts of animal manure, 10-12 parts of potassium sulfate, 20-22 parts of lignite, 0.02-0.03 part of actinomycetes, 0.02-0.03 part of photosynthetic bacteria, 0.04-0.05 part of bacillus, 0.02-0.03 part of aspergillus and 0.06-0.07 part of cellulose decomposition bacteria; the potassium polyacrylate is separately packaged and is mixed with the organic material when applied; during a fermentation period, moisture is controlled at 50-60 percent, and the temperature is controlled at 60 DEG C-70 DEG C; and after the application of the modifying agent, the water-soluble state and full content of Pb, Cd, Cr and the like in soil and vegetables can be reduced greatly.

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

The invention relates to a culture method of a fungus capable of poisoning plant parasitic nematodes, and a preparation method of a metabolite thereof and application thereof, and belongs to the technical field of microbial pesticides. A fungus strain (Aspergillus niger Y-61) related in the invention was collected in China General Microbiological Culture Collection Center on August 7, 2008, and the collection number is CGMCC No.2631. The taxonomic status of the Y-61 is a mitosporic fungus, hyphomycetes, moniliales, moniliaceae, aspergillus and aspergillus niger. The fungus metabolite is prepared by liquid fermentation and culture, has outstanding characteristics of high toxicity to the plant parasitic nematodes, especially root-knot nematodes, and has obvious nematodes killing effect; and the influence of the strain fermentation broth on Meloidogyne incognita juveniles (J2) and oocyst hatching is determined indoors. Treatment of the fermentation broth with different diluted concentrations is significantly different from the aseptic water treatment, and the preventive effect of the fermentation broth with less than one-fifth concentration is be equivalent to that of 10mug / ml of cadusafos. The fungus has good application and development prospects.

An air-oxidative type hair dye composition containing a melanin precursor prepared by a process including (A) an oxidation step for converting, into the melanin precursor, a tyrosine or derivative thereof used as a starting substance with an enzyme or cell that is derived from a fungus selected from the group consisting of fungi belonging to the genera Aspergillus, Neurospora, Rhizomucor, Trichoderma, and Penicillium and that exhibits a catechol oxidase activity.

The invention discloses a method for solid fermentation preparing Gamma-aminobutyric acid comprising the following steps firstly filtering red aspergillus from beancurd preserved, putting the red aspergillus MP1104 on oblique plane culture medium and culturing for 7 days to activate the fungus, then transferring the fermentation fungus into culture medium at 30 DEG C, at rotating speed of 150r / min and culturing the activating fungus rocketing bed for 2 says to prepare fermentation seed, optimizing solid fermentation condition and culture medium, at last culturing and preparing GABA on the condition of optimizing culture medium and culture condition. The fermentation raw material is rice and the fungus is red aspergillus. The product of the invention is safe in edibility and also can be used as health care food. The GABA field and purity in the method are high. In optimum fermentation condition and culture medium, the GABA field can be improved from 0.21mg / g to 0.35mg / g and the final purity can be up to 45, and can be prepared continuously in industrialization in low cost.

The invention relates to the technical field of a biological agent, and particularly relates to a cellulose degradation bacteria agent and a raw material strain, and preparation methods and applications of the cellulose degradation bacteria agent and raw material strain. The compound bacteria agent for degrading cellulose mainly comprises a paecilomyces varioti MM2 strain, an aspergillus fumigates MM6 strain, a candida rugosa MJ4 strain, and a bacillus licheniformis MX8 strain. The cellulose can be efficiently degraded and utilized by the compound bacteria agent disclosed by the invention, the compound bacteria agent can be used as a detonating agent and an enhancer of wine sediment compost, the compost process can be accelerated, the compost rotten degree is improved, an organic fertilizer is fabricated, and the cellulose degradation bacteria agent is especially applicable to resourceful treatment of lignocelluloses solid waste and production of the organic fertilizer by using the wine sediment.

The invention relates to a composite microbial deodorant for treating household garbage and a preparation method of the deodorant. The composite microbial deodorant is prepared from raw materials as follows: 20-30 parts of composite photosynthetic bacterium powder, 10-20 parts of Bacillus subtilis powder, 20-40 parts of composite aspergillus powder and 30-50 parts of composite yeast powder. According to the composite microbial deodorant, hydrogen sulfide, ammonia as well as strains adapted to the household garbage are screened and degraded in a targeted manner, the composite microbial deodorant comprises beneficial microbes such as Thiocapsa roseopersicina capable of efficiently degrading the hydrogen sulfide, Rhodospirillum rubrum capable of efficiently degrading fatty acid series odors and the ammonia, Aspergillus oryzae rich in enzyme systems such as proteases, cellulose and the like, Bacillus subtilis capable of quickly degrading the ammonia, Rhizopus oryzae rich in lactic acid, Saccharomyces cerevisiae for producing ethyl alcohol, Candida guilliermondii rich in aroma-producing substances and the like, the beneficial microbes grow and breed in the household garbage and have the synergistic effect, the ecological environment of the household garbage is effectively improved, and emissions of the hydrogen sulfide, the ammonia and the fatty acid series odors are greatly decreased.