315 results about "Aspergillus" patented technology

The invention relates to the field of microorganisms, and discloses an aspergillus cristatus strain and application thereof. The preservation number of the aspergillus cristatus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41727. The aspergillus cristatus provided by the invention can produce chymosin at high yield, has excellent curding activity and relatively low proteolytic activity, and can reduce the bitter taste of dairy products and improve the yield; the aspergillus cristatus provided by the invention not only can shorten the fermentation time of the dairy product, improve the cream flavor of the dairy product, reduce the peculiar smell of the dairy product, balance the sourness and sweetness of the dairy product and improve the cinnamon flavor, but also has intestinal colonization ability, and has the potential of improving the flavor level of the dairy product and becoming intestinal probiotics.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention discloses a plant-microorganism combined uranium pollution treatment method which comprises the following steps: planting seeds of disinfected uranium-tolerant plants into soil to be remediated, and applying a bacterial suspension of uranium-tolerant strains, namely rhodotorula M1, aspergillus M2 and bacillus M3, into the soil to be remediated, so that the uranium enrichment capacity of the uranium-tolerant plants is remarkably improved; and a relatively good effect of repairing the uranium-polluted soil in the medium-temperature climate region is achieved. Uranium-tolerant plants can be harvested manually or mechanically and transferred to a safe place in a centralized manner to be treated, so that uranium-contaminated soil in the medium-temperature climate zone is effectively repaired.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses application of brevibacillus laterosporus in degradation of ochratoxin A, and belongs to the technical field of microbiology and biodegradation. The preservation number of the brevibacillus laterosporus is CCTCC NO: M 2022932, the brevibacillus laterosporus can degrade the ochratoxin A and prepare a product for degrading the ochratoxin A, the brevibacillus laterosporus participates in degradation of the ochratoxin A in the form of viable bacteria or fermentation liquor, and the degradation rate of the brevibacillus laterosporus to the ochratoxin A is as high as 98.26%. Besides, tests prove that the brevibacillus laterosporus has an antagonistic effect on the aspergillus ochraceus and can be used for preparing an antibacterial product of the aspergillus ochraceus, so that the ochratoxin A is treated from the source.

The invention provides a recombinant Aspergillus niger strain with high yield of trans-aconitic acid, and belongs to the technical field of gene engineering. The recombinant aspergillus niger strain can simultaneously express aconitase and aconitic acid isomerase. According to the genetic engineering aspergillus niger strain provided by the invention, the trans-aconitic acid fermentation yield is generally higher than that of an original strain, and the strain with the highest yield can reach 51g / L on a shake flask level, so that the genetic engineering aspergillus niger strain has very high application value.

The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention provides a microbial agent capable of degrading lignin and application of the microbial agent in regulation and control of a soil organic carbon library, and relates to the technical field of microorganisms, the microbial agent contains at least two of serratia marcescens, ochrobactrum anthropi, pseudomonas asparagi or burkholderia terricola, and the microbial agent contains at least two of the serratia marcescens, the ochrobactrum anthropi, the pseudomonas asparagi and the burkholderia terricola. The serratia marcescens a26, the ochrobactrum anthropi b1, the pseudomonas asparagi b6 or the burkholderia terricola b21 have good lignin degradation capability, after compounding, the lignin degradation capability of the strain is further improved, and the strain can be used for degrading moso bamboo litters with high lignin content and promoting accumulation of a soil organic carbon library. The technical problem that a microbial agent with a function of efficiently degrading lignin is lacked in the prior art is solved.

The invention relates to the technical field of daily chemicals, in particular to a cacumen biotae fermentation filtrate, a caviar and cacumen biotae nano-coated slow-release composition, shampoo and application. The fermentation filtrate is prepared from cacumen biotae through a liquid fermentation method; wherein the used strain is bacillus and / or aspergillus. The cacumen biotae fermented filtrate provided by the invention contains flavonoid compounds (such as quercetin) and volatile oil (such as cacumen biotae ketone) which can improve scalp blood circulation, nourish hair follicles and promote healthy growth of hair. The shampoo can inhibit hair follicle inflammation and prevent hair follicle atrophy, thereby reducing alopecia. Tannin components can balance grease secretion of scalp, and hair follicle blockage caused by excessive grease is avoided. The shampoo is particularly friendly to oily scalp and is beneficial to keeping the scalp fresh and cool. Polysaccharide and flavonoid substances in the cacumen biotae can prolong the hair growth period and stimulate the activity of hair follicles, so that new hair regeneration is promoted. The plant extract mildly stimulates scalp and does not damage hair follicle tissues.

The invention provides a hydroponic symbiotic microbial system and a hydroponic symbiotic method, and relates to the technical field of microbiology. The hydroponic symbiotic microbial system comprises an arbuscular mycorrhizal fungus community, broom cladosporium, aspergillus, pseudomonas and blastomonas. According to the method, a microbial structure in a natural environment is simulated by constructing diversified microbial communities, so that the synergistic effect of the microbial communities is fully exerted; through interaction of modes of co-decomposing organic matters, promoting nutrient absorption, promoting biological control, enhancing plant stress resistance and the like, the stability of an ecological system is co-maintained, and the growth of plants is promoted.

The invention relates to the discovery of a biosynthetic gene cluster of an alkaloid, namely, exopoid C, and the biosynthetic gene cluster of the exopoid. A biosynthetic gene cluster exo of an exopoid compound is obtained from a fungus Penicillium sp.HDN14-431 through genome mining, the gene cluster comprises a heme-dependent oxidase gene exoA which is composed of indole dioxygenase and a cytochrome b5 structural domain, a cytochrome P450 enzyme gene exoB and an isopentenyl transferase gene exoC which is composed of indole dioxygenase and a cytochrome b5 structural domain, and the cytochrome P450 enzyme gene exoB and the isopentenyl transferase gene exoC are used as biosynthetic genes of the exopoid compound. All genes in a gene cluster are expressed through an aspergillus nidulans heterologous expression system, two isopentenyl indole alkaloids are obtained in total, and a compound I is a new compound exopoid C. And the compound II, namely the exopoid, is a known compound. The biosynthesis pathway of the compound is reported for the first time.

Provided is a transfersome cosmetic composition including: a Matricaria flower extract, a Centella asiatica extract, an Arnica montana flower extract, a Scutellaria baicalensis extract, a Paeonia suffruticosa root extract, an aloe vera leaf extract, a Saccharina japonica extract, a licorice root extract, a lactobacillus lysate formed by fermenting strains belonging the lactobacillus genus, a rice bran fermentation filtrate formed by fermenting rice bran using a first composite strain including strains belonging to the bacillus genus, strains belonging to the lactobacillus genus, and yeast, a green tea fermentation extract formed by fermenting green tea using a second composite strain including strains belonging to the Aspergillus genus and strains belonging to the Lactobacillus genus, a grape seed oil, an jojoba seed oil, hydroxyacetophenone, Bifidobacterium, arginine, betaine, trehalose, sodium hyaluronate, hydrolyzed elastin, Ceramide NP, resveratrol, Genistein, tocopheryl acetate, glycerin, and purified water.

The invention discloses a method for generating rare ginsenoside Rh2, which comprises the following steps: adding common glycol type ginsenoside into aspergillus brazilis fermentation liquor for fermentation, and sterilizing after fermentation to obtain a fermentation product; and carrying out enzymolysis by using beta-glucanase and pectinase, and carrying out enzyme deactivation after the enzymolysis is finished, so as to obtain the rare ginsenoside Rh2. The invention also provides the rare ginsenoside Rh2 prepared by the method and application of the rare ginsenoside Rh2. The common glycol type ginsenoside is efficiently and quickly converted into the rare ginsenoside Rh2 through the synergistic interaction of the aspergillus brazilis, the beta-glucanase and the pectinase; the rare ginsenoside Rh2 has the advantages of being simple in preparation method, easy to implement, low in cost, environmentally friendly, free of pollution and the like, and the generated rare ginsenoside Rh2 shows excellent skin care and repairing effects and has wide market prospects when applied to cosmetics.

The present invention discloses a γ-arylbutenolide compound, a preparation method thereof, and an application thereof in the preparation of cardiovascular protective drugs. The above-mentioned structurally novel γ-arylbutenolide compound is isolated for the first time from the fermentation culture of the marine herbal soft coral symbiotic fungus Aspergillus sp. EGF7-0-1, and it is found for the first time that the above-mentioned compound has a protective effect on the cardiovascular system, providing a pharmacodynamic substance basis for the subsequent research on cardiovascular diseases.

To provide a novel technique which enables efficient production of mycelium in a short time.SOLUTION: The present invention provides a method for producing mycelium of koji mold, the method including a culture step involving liquid-culture of koji mold in a culture medium containing grains, and a recovery step involving recovery of the mycelium obtained in the culturing step.SELECTED DRAWING: None

The invention provides an aspergillus terreus JM-F5-6 strain and application thereof in promoting tomato growth and improving soil nutrients, and belongs to the technical field of microorganisms. The preservation number of the aspergillus terreus JM-F5-6 is CCTCC (China Center For Type Culture Collection) NO: M20232616. The conidia of the aspergillus terreus JM-F5-6 are spherical or oval, the surface is smooth, hyphae are colorless or slightly colored, the wall is thick, the hyphae are separated, and hypha branches are laterally connected to form a mesh topology. The aspergillus terreus JM-F5-6 has high protein decomposition capacity and acid production capacity, is beneficial to improving the soil environment, can enhance the photosynthesis and nitrogen absorption of plants and improve the health and growth situation of the plants, effectively improves the content of soil hydrolytic nitrogen, available phosphorus, quick-acting potassium and organic matter, improves the soil fertility, and has wide market application prospects.

The invention discloses a pyranopyridone alkaloid compound as well as a preparation method and application thereof, and belongs to the technical field of medicines. The pyranopyridone alkaloid compound with the activity of resisting the acetaminophen-induced acute liver injury is extracted from a fermentation product of a marine fungus Aspergillus aculeatusWHUF0198, and the pyranopyridone alkaloid compound improves the cell survival rate of mouse hepatocytes treated by acetaminophen and reduces the glutamic-pyruvic transaminase level in a cell supernatant. The pyranopyridone alkaloid compound has the advantages that the pyranopyridone alkaloid compound has the activity of resisting the acetaminophen-induced acute liver injury, and the pyranopyridone alkaloid compound has the activity of resisting the acetaminophen-induced acute liver injury; the pyran pyridone alkaloid compound disclosed by the invention can be used for preparing a medicine for treating acute liver injury. Aiming at the current situation that the types of medicines for treating acute liver injury caused by acetaminophen are deficient at present, the traditional Chinese medicine composition is rich in potential economic benefit.

The invention provides a method for deeply degrading penicillin V through combined circulating cascade of paracoccus and aspergillus niger and application of the method, and belongs to the technical field of antibiotic pollutant degradation, paracoccus is paracoccus sp KDSPL-02, aspergillus niger is Aspergillus niger, and the method is used for deeply mineralizing and degrading penicillin V to dissolve possible pollution caused by the paracoccus sp KDSPL-02 and the aspergillus niger is Aspergillus niger. Waste hyphae generated in the production process are subjected to harmless treatment.

The invention provides aspergillus scherweri for high-yield cellulase and application of the aspergillus scherweri in fermentation of Chinese herbal medicines. The Aspergillus scherweri J-10 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Aspergillus scherweri J-10 is CGMCC No. 41630. The Aspergillus scherweri J-10 disclosed by the invention has the advantages that the Aspergillus scherweri J-10 can be used for preparing the Aspergillus When the aspergillus scherweri provided by the invention is used for fermenting Chinese herbal medicines represented by lucid ganoderma, the release and utilization rate of active ingredients of the Chinese herbal medicines can be remarkably improved. An in-vitro anti-tumor activity result shows that the alcohol extract of the Chinese herbal medicine (represented by ganoderma lucidum sporocarp) fermented by the J-10 strain has a stronger in-vitro inhibition effect on various tumor cells, and a new method and way are provided for deep processing and comprehensive utilization of the Chinese herbal medicine.

The invention provides plant fermented oil with moistening and soothing effects and a preparation method thereof. The preparation method comprises the following steps: fermenting aspergillus in a fermentation culture medium 1, adding candida albicans, inoculating into a fermentation culture medium 2, carrying out primary fermentation under the ventilation capacity of 2-4 L / min, carrying out secondary fermentation under the ventilation capacity of 0.2-0.5 L / min, separating, and sterilizing to obtain the plant fermented oil. The aspergillus and candida mellifera are mixed and fermented according to a specific sequence, so that the obtained plant fermented oil has excellent soothing and moistening effects. Besides, compared with products obtained through fermentation in other modes, the plant fermentation oil has more excellent soothing and moistening effects and is very suitable for being used as and / or used for preparing cosmetics or medical compositions.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention provides a spiropolyspora pink and selenium compound nutrient solution and application thereof in biological prevention and control and growth promotion of tomatoes, the prevention and control effects of different treatment combinations on tomato gray mold are studied, disease indexes and prevention and control effects of different treatment combinations are compared, activity changes of stress-resistant related enzymes SOD, POD and MDA are compared, and the disease resistance of the tomato gray mold is improved. A photosynthetic related index is chlorophyll content. The incidence rate of the spiropolyspora roseum and the selenium-enriched nutrient solution in the treatment group is lowest, the prevention and treatment effect is optimal, and the chlorophyll content is highest, so that the compounding agent can obviously improve the photosynthesis of the plants. Different compounding agents have an induction effect on the disease resistance of tomatoes. The selenium content of the compounded tomatoes is obviously higher than that of a control group and a single selenium-rich nutrient solution, the ratio of the optimal selenium-rich combination to the selenium-rich nutrient solution is 2: 3, the selenium content of tomato fruits can reach 52.67 g / kg, and it is indicated that the selenium absorption of the tomatoes can be promoted through the spiropolyspora pink.

The invention relates to a microbial composition for preventing and treating eggplant rhizome diseases and application thereof, and the composition comprises a) Bacillus velezensis and Aspergillus oryzae, and b) fulvic acid, alginic acid, amino acid, rape pollen polysaccharide, oyster peptide, berberine and potassium phosphite. Wherein the preservation number of the bacillus velezensis is CCTCC (China Center For Type Culture Collection) NO: M2022923, and the preservation number of the aspergillus oryzae is CCTCC NO: M2021249. The composition provided by the invention has a relatively good prevention and control effect on root and stem diseases caused by infection of Ralstonia solanacearum, Rhizoctonia solani, Verticillium dahlii, Fusarium solani, Choanephra cucurbitarum and Basidiomycetes, and when the composition is applied to soil, the enzyme activity of the soil and the enzyme activity of the root system of the eggplant can be effectively improved. The composition provided by the invention has a relatively good prevention and control effect on the root and stem diseases caused by infection of the Ralstonia solanacearum, the Rhizoctonia solani, the Verticillium dahlii, the Fusarium solani, the Choanephra cucurbitarum and the Basidiomycetes.

The invention discloses a method for detecting exogenous amylase derived from aspergillus in honey and application of the method. The method comprises the following steps: screening and determining a characteristic peptide fragment, pre-treating a sample, making a standard curve, separating by using liquid chromatography, detecting by using tandem mass spectrometry, and quantifying by using an internal standard method. The exogenous amylase characteristic peptide fragment derived from aspergillus is FASYTNDIALAK, and the specific peptide fragment derived from aspergillus is FASYTNDIALAK. The invention establishes a quantitative method for detecting the exogenous amylase derived from aspergillus in the honey by liquid chromatography-tandem mass spectrometry, and the quantitative method has better accuracy and sensitivity and is suitable for adulteration identification of the honey. The method has important practical significance for controlling the quality of honey and maintaining the healthy and sustainable development of the bee product industry.

A mixture includes a nickel-containing ore and / or concentrate thereof and an extraction composition including a fermentation broth obtained from an Aspergillus species (spp.), Acetobacter spp., Bacillus spp., Propionibacterium spp., Corynebacterium spp., Rhizopus spp., Clostridium spp., Fusobacterium spp., Pseudomonas spp., Bifidobacterium spp., Saccharomyces spp., Enterobacter spp., Escherichia spp., among others. The extraction composition is configured to selectively solubilize one or more components, such as a magnesium component from the nickel-containing ore and / or concentrate thereof. A method for extracting a metal from a nickel-containing ore and / or concentrate includes selectively solubilizing a magnesium component from the nickel-containing ore and / or concentrate thereof into an extraction composition including the fermentation broth. The method can include extracting a magnesium component from a nickel-containing ore and / or concentrate thereof into an extraction composition, separating the nickel-containing ore and / or concentrate thereof from an extracted magnesium component and broth, separating the broth from the extracted magnesium component, and repeating the extracting. The extracted magnesium component has a purity in a range from 10% to 90%.

The invention belongs to the field of microorganisms, and particularly discloses Aspergillus nomiae for preventing and controlling spodoptera frugiperda. The technical problem to be solved by the invention is how to prevent noctuid insects from harming plants. The invention provides aspergillus for preventing and treating noctuid insects from harming plants, the aspergillus is Aspergillus nomiae WNAN1, and the preservation number of the aspergillus nomiae is CGMCC (China General Microbiological Culture Collection Center) No.41741. The invention further provides a preparation method of the aspergillus nomiae. Experiments prove that the aspergillus has a relatively high fatality rate on third-instar larvae of the spodoptera frugiperda, and can be used for preventing and controlling the spodoptera frugiperda.

An Aspergillus sp. DH4 with aerobic denitrification enhanced by an inorganic electron donor and its application are provided, which relates to the field of microbial technologies. The Aspergillus sp. DH4 is preserved at China Center for Type Culture Collection (CCTCC), a preservation address is Wuhan University, 299 Bayi Road, Wuchang District, Wuhan City, China, a preservation number is CCTCC NO: M20232690, and a preservation date is Dec. 27, 2023.

The application discloses a polyketide compound and a preparation method and application thereof. The polyketide compound is isolated from a fermentation product of Aspergillus taichungensis SMU01. The Aspergillus taichungensis SMU01 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO:62752, and the preservation date is August 30, 2022. The polyketide compound has the effects of promoting Th9 cell proliferation, increasing IFN-gamma and TNF-a secretion, and increasing IL-9 expression, and can be effectively used to enhance the anti-tumor effect of Th9 cells, and has important value for the development and application of anti-tumor drugs.

The invention provides a low-sensitization whey protein hydrolysate as well as a preparation method and application thereof, and the preparation method of the low-sensitization whey protein hydrolysate comprises the following steps: adjusting the pH value of whey liquid to be alkaline, then adding protease, and carrying out heat-preservation hydrolysis, the protease is selected from at least one of bacillus licheniformis alkaline protease, aspergillus oryzae neutral protease and aspergillus mellitus protease. At least one of the bacillus licheniformis alkaline protease, the aspergillus oryzae neutral protease and the honey aspergillus protease is adopted to hydrolyze the whey liquid, unique active ingredients of protein hydrolysate can be enriched, and therefore the problem that existing protein hydrolysate is high in sensitization is effectively solved, excessive hydrolysis is avoided, and the product quality is improved. The nutritional value of protein is reserved, the crowd acceptability is high, the problem that special crowds are allergic or intolerant to cow milk protein is effectively solved, the method is simple, the raw materials are easy to obtain, and the cost is low.