227 results about "Aspergillus" patented technology

The invention relates to the field of microorganisms, and discloses an aspergillus cristatus strain and application thereof. The preservation number of the aspergillus cristatus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41727. The aspergillus cristatus provided by the invention can produce chymosin at high yield, has excellent curding activity and relatively low proteolytic activity, and can reduce the bitter taste of dairy products and improve the yield; the aspergillus cristatus provided by the invention not only can shorten the fermentation time of the dairy product, improve the cream flavor of the dairy product, reduce the peculiar smell of the dairy product, balance the sourness and sweetness of the dairy product and improve the cinnamon flavor, but also has intestinal colonization ability, and has the potential of improving the flavor level of the dairy product and becoming intestinal probiotics.

The invention discloses application of brevibacillus laterosporus in degradation of ochratoxin A, and belongs to the technical field of microbiology and biodegradation. The preservation number of the brevibacillus laterosporus is CCTCC NO: M 2022932, the brevibacillus laterosporus can degrade the ochratoxin A and prepare a product for degrading the ochratoxin A, the brevibacillus laterosporus participates in degradation of the ochratoxin A in the form of viable bacteria or fermentation liquor, and the degradation rate of the brevibacillus laterosporus to the ochratoxin A is as high as 98.26%. Besides, tests prove that the brevibacillus laterosporus has an antagonistic effect on the aspergillus ochraceus and can be used for preparing an antibacterial product of the aspergillus ochraceus, so that the ochratoxin A is treated from the source.

The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention provides a microbial agent capable of degrading lignin and application of the microbial agent in regulation and control of a soil organic carbon library, and relates to the technical field of microorganisms, the microbial agent contains at least two of serratia marcescens, ochrobactrum anthropi, pseudomonas asparagi or burkholderia terricola, and the microbial agent contains at least two of the serratia marcescens, the ochrobactrum anthropi, the pseudomonas asparagi and the burkholderia terricola. The serratia marcescens a26, the ochrobactrum anthropi b1, the pseudomonas asparagi b6 or the burkholderia terricola b21 have good lignin degradation capability, after compounding, the lignin degradation capability of the strain is further improved, and the strain can be used for degrading moso bamboo litters with high lignin content and promoting accumulation of a soil organic carbon library. The technical problem that a microbial agent with a function of efficiently degrading lignin is lacked in the prior art is solved.

The invention discloses a method for generating rare ginsenoside Rh2, which comprises the following steps: adding common glycol type ginsenoside into aspergillus brazilis fermentation liquor for fermentation, and sterilizing after fermentation to obtain a fermentation product; and carrying out enzymolysis by using beta-glucanase and pectinase, and carrying out enzyme deactivation after the enzymolysis is finished, so as to obtain the rare ginsenoside Rh2. The invention also provides the rare ginsenoside Rh2 prepared by the method and application of the rare ginsenoside Rh2. The common glycol type ginsenoside is efficiently and quickly converted into the rare ginsenoside Rh2 through the synergistic interaction of the aspergillus brazilis, the beta-glucanase and the pectinase; the rare ginsenoside Rh2 has the advantages of being simple in preparation method, easy to implement, low in cost, environmentally friendly, free of pollution and the like, and the generated rare ginsenoside Rh2 shows excellent skin care and repairing effects and has wide market prospects when applied to cosmetics.

The invention provides a method for deeply degrading penicillin V through combined circulating cascade of paracoccus and aspergillus niger and application of the method, and belongs to the technical field of antibiotic pollutant degradation, paracoccus is paracoccus sp KDSPL-02, aspergillus niger is Aspergillus niger, and the method is used for deeply mineralizing and degrading penicillin V to dissolve possible pollution caused by the paracoccus sp KDSPL-02 and the aspergillus niger is Aspergillus niger. Waste hyphae generated in the production process are subjected to harmless treatment.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention provides a spiropolyspora pink and selenium compound nutrient solution and application thereof in biological prevention and control and growth promotion of tomatoes, the prevention and control effects of different treatment combinations on tomato gray mold are studied, disease indexes and prevention and control effects of different treatment combinations are compared, activity changes of stress-resistant related enzymes SOD, POD and MDA are compared, and the disease resistance of the tomato gray mold is improved. A photosynthetic related index is chlorophyll content. The incidence rate of the spiropolyspora roseum and the selenium-enriched nutrient solution in the treatment group is lowest, the prevention and treatment effect is optimal, and the chlorophyll content is highest, so that the compounding agent can obviously improve the photosynthesis of the plants. Different compounding agents have an induction effect on the disease resistance of tomatoes. The selenium content of the compounded tomatoes is obviously higher than that of a control group and a single selenium-rich nutrient solution, the ratio of the optimal selenium-rich combination to the selenium-rich nutrient solution is 2: 3, the selenium content of tomato fruits can reach 52.67 g / kg, and it is indicated that the selenium absorption of the tomatoes can be promoted through the spiropolyspora pink.

The invention discloses a method for detecting exogenous amylase derived from aspergillus in honey and application of the method. The method comprises the following steps: screening and determining a characteristic peptide fragment, pre-treating a sample, making a standard curve, separating by using liquid chromatography, detecting by using tandem mass spectrometry, and quantifying by using an internal standard method. The exogenous amylase characteristic peptide fragment derived from aspergillus is FASYTNDIALAK, and the specific peptide fragment derived from aspergillus is FASYTNDIALAK. The invention establishes a quantitative method for detecting the exogenous amylase derived from aspergillus in the honey by liquid chromatography-tandem mass spectrometry, and the quantitative method has better accuracy and sensitivity and is suitable for adulteration identification of the honey. The method has important practical significance for controlling the quality of honey and maintaining the healthy and sustainable development of the bee product industry.

The invention belongs to the field of microorganisms, and particularly discloses Aspergillus nomiae for preventing and controlling spodoptera frugiperda. The technical problem to be solved by the invention is how to prevent noctuid insects from harming plants. The invention provides aspergillus for preventing and treating noctuid insects from harming plants, the aspergillus is Aspergillus nomiae WNAN1, and the preservation number of the aspergillus nomiae is CGMCC (China General Microbiological Culture Collection Center) No.41741. The invention further provides a preparation method of the aspergillus nomiae. Experiments prove that the aspergillus has a relatively high fatality rate on third-instar larvae of the spodoptera frugiperda, and can be used for preventing and controlling the spodoptera frugiperda.

An Aspergillus sp. DH4 with aerobic denitrification enhanced by an inorganic electron donor and its application are provided, which relates to the field of microbial technologies. The Aspergillus sp. DH4 is preserved at China Center for Type Culture Collection (CCTCC), a preservation address is Wuhan University, 299 Bayi Road, Wuchang District, Wuhan City, China, a preservation number is CCTCC NO: M20232690, and a preservation date is Dec. 27, 2023.

The application discloses a polyketide compound and a preparation method and application thereof. The polyketide compound is isolated from a fermentation product of Aspergillus taichungensis SMU01. The Aspergillus taichungensis SMU01 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO:62752, and the preservation date is August 30, 2022. The polyketide compound has the effects of promoting Th9 cell proliferation, increasing IFN-gamma and TNF-a secretion, and increasing IL-9 expression, and can be effectively used to enhance the anti-tumor effect of Th9 cells, and has important value for the development and application of anti-tumor drugs.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.

This application relates to a terphenylamine skeleton compound, its preparation method, and its application, belonging to the field of chemical synthesis technology. The preparation method of the terphenylamine skeleton compound of this application includes the following steps: dissolving 2,4,6-triphenylaniline with thiazole-4-carboxaldehyde or thiophene-3-carboxaldehyde in a solvent, conducting a reductive amination reaction under reducing agent conditions, quenching, extraction, washing, concentration to remove solvent, and purification to obtain the terphenylamine skeleton compound. This application provides a simple and mild synthetic method for the natural active products 5'-phenyl-N-(thiazol-4-ylmethyl)-[1,1':3',1''-terphenyl]-2'-amine and 5'-phenyl-N-(thiophene-3-ylmethyl)-[1,1':3',1''-terphenyl]-2'-amine, which can only be extracted from the secondary metabolites of the marine fungus Aspergillus sp. Furthermore, this method utilizes widely available raw materials, which is beneficial for large-scale industrial production of these natural active products.

The application belongs to the technical field of microbial fermentation, and particularly relates to a kind of cytolysis product of mixed bacteria fermentation and a preparation method. The cytolysis product is prepared by mixed bacteria fermentation of two kinds of aerobic probiotics, i.e. Lactobacillus plantarum and Aspergillus oryzae are used as fermentation strains to carry out mixed bacteria fermentation treatment on rice milk, and membrane filtration and column chromatography system are used for filtration and enrichment, so as to obtain a cytolysis fermentation product filtrate rich in kojic acid, the content of kojic acid is greater than 200 g / L, which is significantly improved compared with conventional process. The obtained cytolysis product has no bad smell and clear color, so that the beneficial function of kojic acid can be played only with a low addition amount in cosmetics, and the addition of odor masking and color correcting auxiliaries is reduced, and the cytolysis product can be used for preparing cosmetics with skin repair, whitening and other functions.

The application discloses a kind of fermentation complex microbial inoculant and preparation method and application thereof, the fermentation complex microbial inoculant includes the following weight parts of component: bacillus subtilis microbial inoculant 2-5 parts, bacillus mucilaginosus microbial inoculant 5-10 parts, yeast microbial inoculant 2-10 parts, trichoderma harzianum microbial inoculant 10-20 parts, aspergillus niger microbial inoculant 10-20 parts and streptococcus thermophilus microbial inoculant 2-5 parts;The fermentation complex microbial inoculant of the application is prepared by selecting each strain and amount, is added to pigeon manure and is fermented to prepare organic fertilizer, can better decompose macromolecular substance, significantly improve the organic matter and total nitrogen content in preparation organic fertilizer, and can better improve seed germination rate;In addition, the preparation method of pigeon manure organic fertilizer of the application is simple in process, easy to operate, and convenient for production.

The invention discloses a novel functional microbiome fortified yeast and application thereof in improving the quality of Shanxi mature vinegar. The invention provides a combined bacterium, which is prepared from Aspergillus sp. (ACCC 34193), Saccharomycopsis fibulligera (9203) ACCC 21511, Lichtheimia ramosa (5977) ACCC 34192, Bacillus velezensis (9386) ACCC 64506, and Lactobacillus plantarum (9600) ACCC 64531, and the combined bacterium is prepared from the following raw materials: Aspergillus sp. (9177), Saccharomycopsis fibulligera (9203), ACCC 21511, Lichtheimia ramosa (5977), ACCC 64506, and Lactobacillus plantarum (9600). The invention also provides a fortified yeast containing the combined bacteria. Compared with the traditional vinegar koji, the Shanxi mature vinegar prepared by using the strengthened koji disclosed by the invention not only improves the saccharification power, flavor and appearance of the vinegar koji, but also effectively improves the quality, flavor and functional substance content of the mature vinegar.

The present invention relates to the technical field of clinical medicine immunodetection, and discloses an aspergillus galactomannan detection kit and a preparation method thereof, the aspergillus galactomannan detection kit comprises a test paper card and a sample treatment liquid, the test paper card comprises a bottom plate, and a sample pad, a labeling pad A, a labeling pad B, a nitrocellulose membrane and water absorption paper which are sequentially arranged from a sample adding end on the surface of the bottom plate, the labeling pad A is provided with a quantum dot-biotin labeled aspergillus galactomannan monoclonal antibody, the labeling pad B is provided with microspheres coupled with the aspergillus galactomannan monoclonal antibody, and the nitrocellulose membrane is coated with streptavidin and goat anti-mouse IgG. According to the invention, the defect of low sensitivity can be overcome.

This invention discloses a method for processing bamboo shoot residue that synergistically enhances the content of soluble dietary fiber and its bound polyphenols. Using waste bamboo shoot residue as raw material, the fermentation bacteria are all food-grade. Specifically, a multi-strain coupled fermentation is employed, including *Bacillus belye*, *Bacillus natto*, *Trichoderma kangsei*, *Cladosporium*, *Aspergillus pamoateus*, and *Bacillus coagulans*. This significantly alters the ratio and content of soluble and insoluble dietary fiber in the bamboo shoot residue and substantially increases the content of dietary fiber-bound polyphenols, synergistically enhancing the content of soluble dietary fiber and its bound polyphenols, thereby producing high-quality dietary fiber. This invention is simple to operate, low in cost, and allows for easier control of fermentation conditions. It is highly efficient and does not easily damage the non-covalent bonds (hydrogen bonds, electrostatic or ionic bonds, etc.) or covalent bonds (ester bonds, ether bonds, etc.) binding dietary fiber and polyphenols. It also has no significant impact on the pH and temperature of the environment, and can significantly increase the content of soluble dietary fiber and its bound polyphenols in bamboo shoot residue.

The application provides a composite microbial agent, application of the composite microbial agent and a treatment method of high-concentration organic wastewater. The active component of the composite microbial agent provided in the first aspect of the application comprises two or three of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain. The composite microbial agent provided in the first aspect of the application can be used for biological decontamination treatment of high-concentration organic wastewater, so as to realize degradation of organic pollutants in the high-concentration organic wastewater and pH value adjustment, thereby avoiding increase of the load of a municipal sewage treatment system.

The application relates to the technical field of semiconductor nanomaterials, and discloses an environment-friendly zinc sulfide nanoparticle as well as a preparation method and application of the zinc sulfide nanoparticle. The preparation method comprises the following steps: (1) activating Aspergillus oryzae spores, performing liquid fermentation, filtering the liquid fermentation product, washing, collecting solid substances, and obtaining biomass; (2) culturing the biomass in the step (1) in a ZnSO4.7H2O aqueous solution to obtain an Aspergillus oryzae liquid solution containing zinc sulfide nanoparticles; (3) drying the liquid solution obtained in the step (2) to obtain Aspergillus oryzae precipitate containing zinc sulfide nanoparticles; and (4) heating the precipitate in the step (3) at 400-450 DEG C for 4-6 h to obtain zinc sulfide nanoparticles. The application endows the zinc sulfide nanoparticles with excellent biocompatibility, expands the application of the zinc sulfide nanoparticles in the fields of biomedicine, chemical sensors, solar cells and light-emitting diodes, and provides a reference for the environment-friendly and batch production of the zinc sulfide nanoparticles.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The present application relates to a kind of mung bean skin fermentation and its preparation method and application of reinforced anti-oxidation, anti-aging effect, belong to the field of microbial technology.The present application provides a kind of preparation method of mung bean skin fermentation of reinforced anti-oxidation, anti-aging effect, including the following steps: after being mixed with white aspergillus, mung bean skin containing solid-state fermentation medium is carried out aerobic fermentation, after fermentation is ended, it is sterilized and is treated, and the pretreated mung bean skin is obtained;Bifidobacterium is inoculated into the culture medium containing pretreated mung bean skin anaerobic fermentation, and mung bean skin fermentation is obtained.The present application is by mung bean skin as one of the main components of solid-state fermentation medium and white aspergillus aerobic fermentation, so that the cell wall of mung bean skin is degraded, and reducing sugar and polyphenol are released, are used by bifidobacterium, so that the fermentation efficiency of bifidobacterium is improved, and then the content of active ingredient in mung bean skin fermentation is improved, and mung bean skin fermentation is endowed with excellent anti-oxidation, anti-aging effect.

The application discloses application of gynostemma pentaphyllum saponin H combined with azole drugs in preparation of antifungal drugs and the antifungal drugs, and relates to the technical field of antifungal drugs. It is found through systematic in-vitro drug sensitivity test that natural product gynostemma pentaphyllum saponin H combined with specific azole antifungal drugs (especially posaconazole POS) can produce significant synergistic antifungal effect, especially for clinical thorny aspergillus, candida, new cryptococcus and dark fungi. The combination strategy can significantly reduce the minimum inhibitory concentration of azole drugs, reverse drug resistance, and is effective for multi-drug resistant strains (such as candida auris). The application provides an efficient and low-toxicity new combination drug scheme for overcoming the increasingly serious fungal drug resistance problem.

The invention relates to aspergillus versicolor and application thereof, and belongs to the technical field of microbial fermentation. The aspergillus versicolor SC007 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.41829, the preservation date is March 12, 2025, the preservation address is Beijing, China, and the aspergillus versicolor SC007 is classified and named as aspergillus versicolor. The Aspergillus versicolor SC007 and Aspergillus oryzae provided by the invention are jointly used for making koji of soybean sauce and soy sauce, the protease activity of finished koji can be remarkably improved, and the improvement of the enzyme activity of the finished koji enables protein in raw materials to be decomposed more sufficiently, so that the content of amino acid nitrogen in soy sauce mash is improved, and the utilization rate of the raw materials of the soy sauce and the soy sauce is further greatly improved; the aspergillus versicolor SC007 can improve the quality and flavor of the product, and the aspergillus versicolor SC007 cannot inhibit the growth and enzyme production of aspergillus oryzae.

The application discloses a steroid-indole alkaloid hybrid dimer compound, a preparation method thereof and application of the compound in preparation of an antitumor chemotherapy drug sensitizer, and first prepares the steroid-indole diketopiperazine alkaloid hybrid compound from a fungus Aspergillus sp. EGF15-0-3 rice culture medium. The compound is a new skeleton compound first discovered in nature. It is first discovered that the hybrid compound has Tdp1 inhibitory activity and can be applied as a tyrosine-DNA phosphodiesterase 1 (Tdp1) inhibitor. It is first discovered that the hybrid compound has chemotherapy sensitization activity on a tumor chemotherapy drug topotecan TPT, which provides a drug source molecule and data support for development of a new natural antitumor combination inhibitor, and has important significance for development and utilization of marine Aspergillus sp. fungus resources.

The invention discloses an ophthalmology image classification method and device based on global local feature extraction, equipment and a medium, and relates to the technical field of medical image analysis, and the method comprises the steps: obtaining a to-be-classified high-resolution cornea image; inputting the to-be-classified high-resolution cornea image into a preset keratitis image classification model so as to perform fungal image classification on the to-be-classified high-resolution cornea image through the preset keratitis image classification model, and outputting a corresponding aspergillus keratitis image classification result or fusarium keratitis image classification result; wherein the preset keratitis image classification model is an image classification model which learns multi-scale fungus feature extraction capability, fuses image global features and image local features and performs image classification. And fine classification of the two types of fungi is realized through the model.

The invention belongs to the technical field of plant disease control, and particularly relates to a method for controlling root rot of cardamine violifolia. The technical problem to be solved by the invention is to clarify the pathogenic bacteria of cardamine violifolia and provide a new choice for preventing and treating cardamine violifolia root rot. The invention also provides a method for preventing and treating the root rot of cardamine violifolia. The method comprises the following step: treating the cardamine violifolia by adopting at least one of hymexazol (T1), oxaconazole (T2) and propiconazole (T9). According to the application, the key pathogenic bacteria of the root rot of the Enshi cardamine violifolia are determined to be Aspergillus Goddavidii (A2), Mucor circinelloides Luusitanius variant (A3) and Blumeria tuberosa (A6) for the first time. According to the invention, bactericides, namely propiconazole, hymexazol and oxadiazole, which have a remarkable inhibition effect on three pathogenic bacteria are screened from nine conventional bactericides, and the mixed use effect of propiconazole, hymexazol and oxadiazole is better; a new scheme is provided for preventing and treating the root rot of cardamine violifolia.

The invention provides an Aspergillus oryzae-ginseng solid fermentation method and application, and belongs to the technical field of ferment.The Aspergillus oryzae-ginseng solid fermentation method includes activating Aspergillus oryzae strains, preparing Aspergillus oryzae spore suspension and preparing and fermenting Aspergillus oryzae-ginseng solid fermentation substrates. Technical parameters of solid fermentation of aspergillus oryzae and ginseng are optimized by using a single-factor test method. The best fermentation effect is determined under the conditions that the fermentation time is 8 days, the inoculum size is 2.5% and the fermentation temperature is 28 DEG C. The total sugar content and the total saponin content obtained by fermentation are respectively 3.853 + / -0.025 mg.mL <-1 > and 1.687 + / -0.002 mg.mL <-1 >. Compared with a control group, the total sugar content is increased by 2.048 times, and the total saponin content is increased by 1.448 times.