205 results about "Cellulose degradation" patented technology

The invention provides a bacillus subtilis LU5 and a lignocellulose degrading flora as well as a culture method and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain is screened from surface soil of composted straws and identified as bacillus subtilis, the strain is combined with bacillus velezensis SS6 and trichoderma longibrachiatum according to a specific proportion, co-culture is carried out in a specific culture medium, a flora capable of degrading lignocellulose is obtained, all microorganisms of the flora are in synergistic symbiosis, and the effect of degrading lignocellulose is achieved. The produced various cellulases have a synergistic effect, so that the aim of degrading lignocellulose is fulfilled. And by adopting the flora provided by the invention, the efficiency and the stability of the lignocellulose degradation process are remarkably improved.

The invention relates to a salt-tolerant bacillus and application thereof, and belongs to the technical field of microbial fertilizers, the salt-tolerant bacillus is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 10, 2024, and the preservation number is CGMCC No.31260; the strain is relatively strong in saline-alkaline tolerance, has the capabilities of dissolving potassium, decomposing cellulose, degrading protein and dissolving organophosphorus, can antagonize various plant pathogenic bacteria such as Erwinia persicae, fusarium oxysporum and fusarium fusiforme, can generate a good growth promoting effect on corn in a salt-free or salt-containing environment, and is very wide in application prospect.

The invention relates to a strain of Prussella reevesii GY-2 and application thereof, and belongs to the technical field of microorganisms, the Prussella reevesii GY-2 simultaneously has the capabilities of fixing nitrogen, degrading protein, degrading cellulose, degrading fat and producing siderophores, has a remarkable growth promoting effect on wheat in a saline-alkali environment, can promote the growth of cucumbers, and has a wide application prospect.

The invention discloses Pseudomonas sp. W50 and an application of the Pseudomonas sp. W50 in degradation of xanthate by combining the Pseudomonas sp. W50 with Cynanchum otophyllum. The Pseudomonas sp. W50 is preserved in Guangdong Microbial Culture Collection Center on July 31, 2024, and the preservation number of the Pseudomonas sp. W50 is GDMCC NO.64924. The Pseudomonas sp. W50 is preserved in the Guangdong Microbial Culture Collection Center on July 31, 2024. The degradation rate of 100mg / L BuX in 9h reaches up to 99%, and the strain also has various growth promoting characteristics of dissolving phosphorus, dissolving potassium, secreting IAA and GA and degrading cellulose. The BuX-polluted wastewater remediation agent can be used for remediation of BuX-polluted wastewater, can play a synergistic interaction role when being combined with pinkflower clover herb, has a more remarkable remediation effect, provides a new method for treatment of BuX pollution and promotion of healthy growth of aquatic plants in mining areas, and has a wide application prospect.

The invention discloses an alpha-glucan phosphorylase mutant as well as a preparation method and application thereof. Aiming at the problems of low catalytic efficiency, poor stability and adaptability, difficulty in heterologous expression and the like of a key enzyme element for preparing starch from cellulose, a metagenome and public database big data analysis and bioinformatics method is adopted; novel cellulose degradation and starch synthesis key enzyme elements are accurately excavated, and the alpha-glucan phosphorylase from tobacco is excavated and identified; compared with alpha-glucan phosphorylase from potatoes, the alpha-glucan phosphorylase from tobacco has better enzyme activity and starch synthesis function; according to the invention, a Cap structural domain of alpha-glucan phosphorylase derived from tobacco is further subjected to single-site mutation, and a result shows that the alpha-glucan phosphorylase derived from tobacco with an amino acid sequence as shown in SEQ ID No.1 is subjected to N563T single-site mutation to obtain a single-site mutant; the enzyme activity and the expression quantity of the alpha-glucan phosphorylase are obviously higher than those of wild type alpha-glucan phosphorylase.

The invention discloses an endoglucanase mutant with acid resistance and application of the endoglucanase mutant, and belongs to the technical field of gene engineering and enzyme engineering. According to the present invention, PCR amplification is adopted to carry out site-specific saturation mutation on endoglucanase tCel5A, and n variants S45D / T55D / T59D and T88H / W255H are constructed and screened; the enzyme activity determination result shows that the enzyme activities of the endoglucanase variants S45D / T55D / T59D and T88H / W255H under the acidic condition are 8828.7 U / mg prot and 5206.7 U / mg prot respectively, and the degradation rates of the endoglucanase variants S45D / T55D / T59D and T88H / W255H to vinasse under the solid-to-liquid ratio of 1: 30 are 53.53% and 50.47% respectively, so that the endoglucanase variants S45D / T55D / T59D and T88H / W255H have high acid resistance and plant cellulose degradation capacity and have great application value in the aspects of agriculture and brewing.

The invention provides a soil conditioner as well as a preparation method and application thereof. The soil conditioner provided by the invention is prepared from the following raw material components in parts by weight: 35 to 45 parts of straw biochar, 55 to 65 parts of biological excrement, 0.01 to 0.5 part of cellulose degradation compound bacteria, 1 to 5 parts of nano silicon dioxide and 3.5 to 4.5 parts of bovine serum albumin. The soil conditioner provided by the invention is good in composting quality, high in composting efficiency, environment-friendly and suitable for industrial production.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses bacillus subtilis WJL-2 and application thereof, and relates to the technical field of microorganisms. The bacillus subtilis WJL-2 disclosed by the invention is preserved in Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC No: 65111, the preservation date is September 9, 2024, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. Results of the embodiment of the invention show that the strain WJL-2 has the capability of degrading cellulose, and when the strain WJL-2 is used for a liquid fermentation test, the degradation rate of the strain WJL-2 on sugarcane straws on the 12th day is 26.45%, and the degradation rate of the strain WJL-2 on straws on the 3rd day of culture reaches 26.37%. The highest hemicellulose degradation rate of the strain WJL-2 is 58.72%, and the highest cellulose degradation rate of the strain WJL-2 is 39.88%.

The invention provides a high-temperature-resistant cellulose degrading bacterium and application thereof, belongs to bacillus (Bacillus), and is named as Bacillus licheniformis C1. The high-temperature-resistant cellulose degrading bacterium has the advantages that the high-temperature-resistant cellulose degrading bacterium can be used for degrading high-temperature-resistant cellulose; according to the invention, continuous enrichment culture is carried out in a cellulose degradation culture medium under a high-temperature condition, a high-temperature-resistant cellulose degradation bacterium C1 is separated, and the strain C1 is a species of bacillus through preliminary identification of multiphase taxonomy. The filter paper enzyme activity of C1 obtained through screening at the temperature of 50 DEG C reaches 14.014 + / -0.438 U / ml, the endo-cellulase activity reaches 1.664 + / -0.225 U / ml, the exo-cellulase activity reaches 3.909 + / -0.374 U / ml, and the C1 can play a role in lignocellulose treatment and fertilizer preparation.

The invention discloses staphylococcus warneri AACE8 capable of promoting the growth of armillaria mellea and application of the staphylococcus warneri AACE8, and relates to the technical field of microorganisms. The invention also discloses an application of the staphylococcus warneri AACE8 in promoting the growth of armillaria mellea. The staphylococcus warneri AACE8 has the capacity of degrading cellulose and producing indole-3-acetic acid, can promote extension and branching of armillaria mellea rhizomorpha, and improves the growth speed, biomass and wood degradation speed of the armillaria mellea rhizomorpha.

The invention discloses an efficient cellulose degrading bacterial agent and a preparation method and application thereof, and belongs to the technical field of microorganisms, the degrading bacterial agent comprises brevundimonas sp., alcaligenes faecalis AFBII and Achromobacter AFBIII, a genome of the brevundimonas AFBI comprises a nucleotide sequence as shown in SEQ ID NO.1, a genome of the AFBI comprises a nucleotide sequence as shown in SEQ ID NO.2, a genome of the AFBII comprises a nucleotide sequence as shown in SEQ ID NO.3, and a genome of the AFBIII comprises a nucleotide sequence as shown in SEQ ID NO.4. The genome of the alcaligenes faecalis AFBII comprises a nucleotide sequence as shown in SEQ ID NO.2, and the genome of the achromobacter AFBIII comprises a nucleotide sequence as shown in SEQ ID NO.3. The alcaligenes faecalis AFBII and the achromobacter AFBIII are used for preparing the alcaligenes faecalis According to the high-efficiency cellulose degrading bacterial agent provided by the invention, all strains have a synergistic effect, and cellulose can be efficiently and stably degraded.

The invention relates to the technical field of agricultural microorganisms, and provides bacillus velezensis HY54 and application thereof. The preservation number of the bacillus velezensis HY54 provided by the invention is CCTCC (China Center For Type Culture Collection): M 20242819, and the bacillus velezensis HY54 has strong adaptability to the environment and shows good growth capability under the conditions of high salt and drought. In addition, the strain also has the capabilities of low temperature resistance, high temperature resistance, growth promotion, disease prevention and control, cellulose degradation and soil heavy metal cadmium and lead pollution resistance, and when the strain is combined with nitrogen-fixing bacteria for use, the crop yield can be increased by 12%, which is significantly superior to that of a single strain treatment group.

The invention relates to the field of microorganisms, in particular to penicillium oxalicum G6 and application thereof. The preservation number of the penicillium oxalicum G6 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41386. The Penicillium oxalicum G6 provided by the invention can quickly start a compost heating process in a low-temperature environment, the high-temperature period is prolonged, the high-temperature peak value of compost is improved, and the generated compost product is highly harmless and non-toxic. Meanwhile, the strain has a relatively strong cellulose degradation function and also has a growth promoting effect, and compared with a commercial fertilizer, the dry weight of the overground part and the underground part of the plant can be effectively increased. Therefore, the strain provided by the invention has multiple functions and is a probiotic with excellent properties.

The invention discloses bacillus subtilis for cellulose degradation and application of the bacillus subtilis in preparation of a bio-organic fertilizer through fermentation of mulberry leaf and chrysanthemum cold granule traditional Chinese medicine residues. The bacillus subtilis is collected and separated from decomposed soil under osmanthus fragrans forests in the bead mountain park in the Dalou City; through a sodium carboxymethyl cellulose culture medium, a Congo red dyeing transparent ring and an enzyme activity determination method, an efficient cellulose degradation strain is screened, and the cellulose degradation strain is inoculated with mulberry leaf and chrysanthemum cold granule traditional Chinese medicine residues, rice hull fungus sticks and pig manure as raw materials for solid state fermentation to produce the bio-organic fertilizer. The cellulose degrading bacterium is high in degrading capacity, and the bio-organic fertilizer prepared by fermenting the traditional Chinese medicine residues through the cellulose degrading bacterium is uniform in size and gap, alkalescent, capable of reducing the content of heavy metal, relieving soil acidification and remarkably increasing the number of soil microbial fungi and bacteria. The bio-organic fertilizer prepared by applying the cellulose degrading bacteria to ferment traditional Chinese medicine residues and livestock wastes can significantly improve the yield and quality of citrus.

The invention discloses a regenerated cellulose industrial filament spinning solution, a preparation method thereof and a preparation method of regenerated cellulose industrial filaments. The preparation method of regenerated cellulose industrial filament spinning comprises the following steps: carrying out alkalization treatment on an ionic liquid aqueous solution; preparing cellulose pulp; premixing the alkalized solution and cellulose pulp; preparing a spinning solution; preparing cellulose industrial filaments from the test lines; under pushing of nitrogen and a booster pump, the test line spinning solution is extruded through a filter and a spinning assembly in sequence, and regenerated cellulose hydrogel filaments are obtained through side blowing cooling and coagulating bath forming; and drawing, washing, drying and rolling the gel to obtain a finished product. According to the regenerated cellulose industrial filament spinning stock solution, the preparation method thereof and the preparation method of the regenerated cellulose industrial filament, high-molecular-weight cellulose pulp can be uniformly dissolved with high quality; the problems of cellulose degradation and high energy consumption caused by temperature rise and dissolution time prolonging in the traditional process are avoided.

The invention belongs to the field of cellulose degradation strains, and particularly discloses bacillus subtilis and application thereof. The bacillus subtilis is classified and named as bacillus subtilis, the name of the bacillus subtilis is bacillus subtilis B-03, the bacillus subtilis is preserved in Guangdong Microbial Culture Collection Center on August 29, 2025, and the preservation number of the bacillus subtilis is GDMCC No: 66896. The invention provides bacillus subtilis B-03 with high cellulose and hemicellulose degradation rate, the cellulose degradation rate of the bacillus subtilis B-03 can reach 75.58%, the hemicellulose degradation rate of the bacillus subtilis B-03 is as high as 97.24%, and the cellulose degradation rate and the hemicellulose degradation rate of the bacillus subtilis B-03 are obviously higher than those of existing cellulose bacillus subtilis degrading bacteria; meanwhile, the method can be suitable for a non-sterilization straw fermentation scene and is high in adaptability, so that the method has a wide application prospect in industrial natural environments such as composting and biogas fermentation.

The invention belongs to the technical field of microorganisms and application thereof, and particularly relates to a low-temperature-resistant complex microbial inoculant capable of degrading lignin and cellulose and application of the complex microbial inoculant in manure fermentation. The microbial inoculum contains lysinibacillus fusiformis LFS1 and bacillus tropicalis BTS1. The strain is preserved in the China General Microbiological Culture Collection Center on October 24, 2024, and the preservation number is GCMCCNO: 32336, No: 32338. The microbial agent disclosed by the invention can accelerate a manure fermentation process under a low-temperature condition, and is beneficial to enriching a low-temperature-resistant strain resource library with lignin and cellulose degradation capabilities. According to the invention, two purified strains are mixed according to different proportions to prepare a bacterial suspension, and a series of high-efficiency complex microbial inoculants are developed, so that lignin and cellulose can be effectively degraded in a low-temperature environment, starting temperature rise of compost is promoted, and the quality of the compost is improved. Compared with traditional methods such as manual heating, the method is higher in efficiency, lower in cost and easier to operate.

The invention discloses low-temperature salt-tolerant and alkali-tolerant streptomyces microflavus and application thereof, relates to the technical field of microorganisms, and is characterized in that the preservation number of the streptomyces microflavus is CCTCC (China Center for Type Culture Collection) M2023664. The streptomyces microflavus provided by the invention can well grow at the temperature of 10-30 DEG C. In a Congo red decoloration test, the ratio of the diameter (D) of a transparent circle to the diameter (d) of a bacterial colony is 3.6. The strain is applied to degradation of cellulose, has the advantages of degradation of cellulose at low temperature, high cellulase activity, salt resistance, alkali resistance and the like, and can be particularly used for cellulose degradation strain resources in low-temperature saline-alkali areas.

The invention belongs to the technical field of biological organic fertilizers, and provides a multifunctional biological organic fertilizer based on agricultural and forestry wastes and a preparation method thereof. The preparation method comprises the following steps: combining chitosan oligosaccharide molecules with sepiolite, and combining a composite bacterial liquid with a chitosan oligosaccharide-sepiolite composite matrix to obtain a chitosan oligosaccharide-sepiolite-composite probiotic carrier; sodium sulfite and epichlorohydrin are utilized to enable the modified lignin to have higher solubility, and the modified lignin is combined with the nano montmorillonite to form a stable network through glutaraldehyde; converting the carbon-rich biomass into biochar, and combining starch, soybean protein and a mixed trace element solution to obtain a composite synergist; lignocellulose of agricultural and forestry wastes is subjected to structural damage, and cellulose and hemicellulose are degraded and a carbon source is provided for subsequent functional microorganisms under the synergistic effect of a compound enzyme solution; in the composting fermentation process, deep degradation of organic matters is achieved, a sodium alginate solution is sprayed to form a stable nutrient slow-release network, and meanwhile the water retention capacity of the particles is improved.

The invention discloses cellulose degradation bacillus subtilis, cellulase secreted by the cellulose degradation bacillus subtilis and application of the cellulose degradation bacillus subtilis, and belongs to the technical field of microorganisms. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 20242073, and the preservation date is September 25, 2024. The enzyme activity of the cellulase secreted and produced by the strain is 10.93 U / mL. The invention also provides application of the strain in degradation of cellulose. The bacillus subtilis has the beneficial effects that the bacillus subtilis with efficient cellulose degradation capability is provided, and the effectiveness of the bacillus subtilis in the aspect of filter paper degradation is verified. The filter paper degradation rate of the strain reaches 28.7%, so that the strain has a better application prospect in the field of cellulose degradation.

The invention discloses a soil remediation and microbiological detection system for agriculture, and relates to the technical field of soil remediation, the system comprises the following components: a straw treatment and microbiological proliferation module, which is used for crushing straws obtained after crops are harvested to 5-10cm and mixing the crushed straws into a position with the depth of 10-20cm on the surface layer of soil; calculating and adding a proper amount of low-temperature cellulose degrading bacteria according to soil and straw conditions, and supplementing nutrient substances to proliferate in the soil; by accurately controlling straw in-situ field returning and microorganism adding, the soil fertility is effectively improved, the soil structure is improved, the content of organic matter and inorganic salt in the soil is increased by means of close combination of low-temperature cellulose degrading bacteria and straw, and by regulating nitrogenous fertilizer operation and applying a decomposition agent in a matched mode, the soil quality is improved. According to the method, the number of soil microorganisms and the population structure are optimized, the soil remediation effect and efficiency are remarkably improved, the problems that soil fertility is reduced and the structure is damaged are solved, and sustainable development of the agricultural ecological environment is further promoted.

The invention discloses an efficient composting and resource utilization method for garden waste. The method comprises the following steps: S1, classification pretreatment: classifying the garden waste into high lignin, medium lignin and low lignin; respectively crushing until the particle size is less than or equal to 5mm (class A) or less than or equal to 3mm; adjusting the initial carbon-nitrogen ratio (C / N) of the mixed material to (35-45): 1 and the initial water content to 55-65%; s2, preparation and inoculation of a complex microbial inoculant: preparing a complex functional microbial inoculant containing a lignin degrading microbial inoculant, a cellulose / hemicellulose degrading microbial inoculant, a function enhancing microbial inoculant and a protective carrier, and inoculating the complex functional microbial inoculant into the pretreated material with the inoculation amount being 0.5-1.0% of the total dry weight of the material. The garden waste is finely classified and crushed according to the lignin content, and the physical structure and the initial C / N of the material are optimized; the white rot fungi, the trichoderma reesei, the bacillus subtilis and other strains in the complex microbial inoculant have a synergistic effect, and particularly, the white rot fungi specifically degrade lignin, so that the degradation bottleneck of a lignocellulose composite structure is effectively broken.

The invention relates to the technical field of microbial fermentation, and discloses a high-protein pig feed prepared by a microbial fermentation technology, which is prepared by the following steps: S1, mixing soybean meal, corn straw and bran according to a mass ratio of 5: 3: 2, crushing to 60-80 meshes, adding a compound microbial agent and water, and adjusting the water content to 45-50% to prepare a fermentation base material; the method comprises the following steps: carrying out first-stage solid-state fermentation for 48-60 hours under the condition of 35-38 DEG C, introducing sterile air in the fermentation process and maintaining the pressure in a tank to be 0.05-0.1 MPa and the stirring speed to be 30-50 rpm, and by arranging a complex microbial inoculant synergistic fermentation system, when the high-protein feed is prepared from soybean meal, corn straws and other plant raw materials, the high-protein feed can be prepared from the soybean meal, the corn straws and other plant raw materials. The specific compatibility proportion of bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae is screened, the synchronous effect of cellulose degradation, organic acid synthesis and mycoprotein enrichment is achieved, and efficient passivation of anti-nutritional factors of different raw materials is guaranteed.

The invention relates to the technical field of microorganisms, in particular to a saline-alkaline-tolerant bacillus velezensis JT6, a cellulose degrading enzyme secreted by the same and application of the saline-alkaline-tolerant bacillus velezensis JT6. The bacillus velezensis JT6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 5, 2025, and the preservation number is CGMCC No.36918. The bacillus velezensis JT6 disclosed by the invention can be used for preparing the bacillus velezensis JT6. The strain has cellulose degradation activity, the degradation rate of corn straw is 48.8%, and the degradation rate of rice straw is 24.92%. The strain provided by the invention is used for rapid decomposition of straw returning, is safe to human and livestock, has no pollution to the environment, and has good development and application prospects.

The present invention discloses Trichoderma asperellum ZC3 and its application in the degradation of Zanthoxylum bungeanum branches. This strain has a fast growth rate and strong degradation ability. In a liquid filter paper strip culture medium, after culturing at 28 °C for 7 days, the filter paper strip completely disintegrates; in a solid culture medium of Zanthoxylum bungeanum branch powder, after culturing at 28 °C for 40 days, its lignin degradation rate for Zanthoxylum bungeanum branches reaches 78.11%, and the degradation rates of cellulose and hemicellulose reach 82.58% and 75.84% respectively. Under a scanning electron microscope, obvious fiber breakage and structural disintegration phenomena can be seen in the Zanthoxylum bungeanum branches, showing a high degradation ability. In addition, the lignin-degrading bacterium ZC3 has good tolerance to the leaching solution of Zanthoxylum bungeanum branches.

The invention relates to the technical field of microorganisms, in particular to the technical field of agricultural microorganisms, and more particularly relates to a cellulose degradation composite flora as well as a construction method and application thereof. The cellulose degradation composite flora comprises a cellulose degradation bacterium, namely humid cellulomonas CW35 and an auxiliary strain used for digesting an intermediate metabolite of the humid cellulomonas CW35, and the auxiliary strain is one or more of pseudomonas Taiwan CW19, bacillus amyloliquefaciens L3 or microbacterium algal M266-2. Therefore, the feedback inhibition effect of a product in the cellulose degradation process of the cellulomonas can be destroyed, the expression and enzyme activity of cellulose degradation related genes are remarkably improved, and the decomposition utilization of cellulose-degrading bacteria, namely the wet cellulomonas CW35, on cellulose-containing wastes is effectively improved.

The invention discloses a method for accelerating weathering and soil formation of waste concrete by microbial fermentation, which comprises the following steps: crushing the waste concrete into blocks with the particle size of 20-50cm, retaining the fracture structure of the blocks, mixing the concrete blocks with biomass, adding 0.3-0.5% of compound microbial agent which specifically comprises acid-producing bacteria, cellulose degrading bacteria and silicate weathering bacteria into the mixed material, and uniformly stirring to obtain a mixture; composting the mixed material, controlling the temperature of the pile body to be 40-55 DEG C, maintaining for at least 15 days, and gradually decomposing the concrete block body by utilizing the synergistic biological weathering effect of organic acid generated by microbial metabolism and the microbial corrosion effect in the fermentation process. According to the method disclosed by the invention, the dynamic bottleneck of decomposition of calcium silicate minerals in concrete blocks is broken through by virtue of synergistic fermentation of the composite flora and the biomass and the coupling effect of organic acid release and biological erosion, and the crushing energy consumption is reduced compared with that of a traditional fine crushing process; and a novel green conversion method integrating rapid soil formation, nutrient activation and pollution prevention and control is formed.

The invention provides a cellulose degrading strain for degrading traditional Chinese medicine residues, a microbial agent and a method for degrading the traditional Chinese medicine residues, and relates to the technical field of microorganisms. The invention provides a cellulose degrading strain for degrading traditional Chinese medicine residues, which comprises Cellulomonas X-5 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.34614, and Cellulomonas X-5 with the preservation number of CGMCC No.34614; the preservation number of the cellulomicrobacterium X-7 is CGMCC (China General Microbiological Culture Collection Center) No.34615 According to the research and verification of the application, both the cellulomonas X-5 and the cellulomicrobacterium X-7 have good fiber degradation activity, especially for multi-medicinal material mixed traditional Chinese medicine residues, the decomposition rate of the traditional Chinese medicine residues can be remarkably improved, and meanwhile, the contents of quick-acting phosphorus and alkali-hydrolyzable nitrogen in the degraded traditional Chinese medicine residues can also be improved. Therefore, the cellulose degrading strain obtained in the invention not only is helpful for realizing resource utilization of agricultural wastes, but also can effectively improve the soil fertility and reduce the risk of environmental pollution.

This invention discloses a strain of *Bacillus parabrachium* ABX1 and its applications, belonging to the field of beneficial microbial technology. The *Bacillus parabrachium* ABX1 provided by this invention is a Gram-positive bacterium isolated from the intestinal fluid of *Bombyx mori*. On CMC-Na solid medium, the ratio of the diameter of the hydrolysis zone to the colony diameter can reach 11. Enzyme activity identification results show that the filter paper enzyme activity of *Bacillus parabrachium* ABX1 is as high as 109.79 U·mL. ‑1 The exo-β-glucanase activity was 107.59 U·mL. ‑1 The cellulase activity reached 107.37 U·mL ‑1 It exhibits excellent cellulose and / or crystalline cellulose degradation capabilities; it is easy to use and has a wide range of enzyme production conditions, showing excellent enzyme activity at pH 5–10 and 25–50℃; the enzyme production time is short, with high enzyme yield achieved in just 12 hours, and there is no significant adverse effect on enzyme yield with prolonged reaction time; it is highly safe to use and has great application prospects in areas such as oak garden waste branch treatment, improvement of oak silkworm digestibility, industrial degradation of cellulose, and improvement of soil fertility.