160 results about "Cellulose degradation" patented technology

The invention relates to a strain of Prussella reevesii GY-2 and application thereof, and belongs to the technical field of microorganisms, the Prussella reevesii GY-2 simultaneously has the capabilities of fixing nitrogen, degrading protein, degrading cellulose, degrading fat and producing siderophores, has a remarkable growth promoting effect on wheat in a saline-alkali environment, can promote the growth of cucumbers, and has a wide application prospect.

The invention discloses Pseudomonas sp. W50 and an application of the Pseudomonas sp. W50 in degradation of xanthate by combining the Pseudomonas sp. W50 with Cynanchum otophyllum. The Pseudomonas sp. W50 is preserved in Guangdong Microbial Culture Collection Center on July 31, 2024, and the preservation number of the Pseudomonas sp. W50 is GDMCC NO.64924. The Pseudomonas sp. W50 is preserved in the Guangdong Microbial Culture Collection Center on July 31, 2024. The degradation rate of 100mg / L BuX in 9h reaches up to 99%, and the strain also has various growth promoting characteristics of dissolving phosphorus, dissolving potassium, secreting IAA and GA and degrading cellulose. The BuX-polluted wastewater remediation agent can be used for remediation of BuX-polluted wastewater, can play a synergistic interaction role when being combined with pinkflower clover herb, has a more remarkable remediation effect, provides a new method for treatment of BuX pollution and promotion of healthy growth of aquatic plants in mining areas, and has a wide application prospect.

The invention provides a soil conditioner as well as a preparation method and application thereof. The soil conditioner provided by the invention is prepared from the following raw material components in parts by weight: 35 to 45 parts of straw biochar, 55 to 65 parts of biological excrement, 0.01 to 0.5 part of cellulose degradation compound bacteria, 1 to 5 parts of nano silicon dioxide and 3.5 to 4.5 parts of bovine serum albumin. The soil conditioner provided by the invention is good in composting quality, high in composting efficiency, environment-friendly and suitable for industrial production.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses an efficient cellulose degrading bacterial agent and a preparation method and application thereof, and belongs to the technical field of microorganisms, the degrading bacterial agent comprises brevundimonas sp., alcaligenes faecalis AFBII and Achromobacter AFBIII, a genome of the brevundimonas AFBI comprises a nucleotide sequence as shown in SEQ ID NO.1, a genome of the AFBI comprises a nucleotide sequence as shown in SEQ ID NO.2, a genome of the AFBII comprises a nucleotide sequence as shown in SEQ ID NO.3, and a genome of the AFBIII comprises a nucleotide sequence as shown in SEQ ID NO.4. The genome of the alcaligenes faecalis AFBII comprises a nucleotide sequence as shown in SEQ ID NO.2, and the genome of the achromobacter AFBIII comprises a nucleotide sequence as shown in SEQ ID NO.3. The alcaligenes faecalis AFBII and the achromobacter AFBIII are used for preparing the alcaligenes faecalis According to the high-efficiency cellulose degrading bacterial agent provided by the invention, all strains have a synergistic effect, and cellulose can be efficiently and stably degraded.

The invention relates to the technical field of agricultural microorganisms, and provides bacillus velezensis HY54 and application thereof. The preservation number of the bacillus velezensis HY54 provided by the invention is CCTCC (China Center For Type Culture Collection): M 20242819, and the bacillus velezensis HY54 has strong adaptability to the environment and shows good growth capability under the conditions of high salt and drought. In addition, the strain also has the capabilities of low temperature resistance, high temperature resistance, growth promotion, disease prevention and control, cellulose degradation and soil heavy metal cadmium and lead pollution resistance, and when the strain is combined with nitrogen-fixing bacteria for use, the crop yield can be increased by 12%, which is significantly superior to that of a single strain treatment group.

The invention belongs to the field of cellulose degradation strains, and particularly discloses bacillus subtilis and application thereof. The bacillus subtilis is classified and named as bacillus subtilis, the name of the bacillus subtilis is bacillus subtilis B-03, the bacillus subtilis is preserved in Guangdong Microbial Culture Collection Center on August 29, 2025, and the preservation number of the bacillus subtilis is GDMCC No: 66896. The invention provides bacillus subtilis B-03 with high cellulose and hemicellulose degradation rate, the cellulose degradation rate of the bacillus subtilis B-03 can reach 75.58%, the hemicellulose degradation rate of the bacillus subtilis B-03 is as high as 97.24%, and the cellulose degradation rate and the hemicellulose degradation rate of the bacillus subtilis B-03 are obviously higher than those of existing cellulose bacillus subtilis degrading bacteria; meanwhile, the method can be suitable for a non-sterilization straw fermentation scene and is high in adaptability, so that the method has a wide application prospect in industrial natural environments such as composting and biogas fermentation.

The invention discloses low-temperature salt-tolerant and alkali-tolerant streptomyces microflavus and application thereof, relates to the technical field of microorganisms, and is characterized in that the preservation number of the streptomyces microflavus is CCTCC (China Center for Type Culture Collection) M2023664. The streptomyces microflavus provided by the invention can well grow at the temperature of 10-30 DEG C. In a Congo red decoloration test, the ratio of the diameter (D) of a transparent circle to the diameter (d) of a bacterial colony is 3.6. The strain is applied to degradation of cellulose, has the advantages of degradation of cellulose at low temperature, high cellulase activity, salt resistance, alkali resistance and the like, and can be particularly used for cellulose degradation strain resources in low-temperature saline-alkali areas.

The invention belongs to the technical field of biological organic fertilizers, and provides a multifunctional biological organic fertilizer based on agricultural and forestry wastes and a preparation method thereof. The preparation method comprises the following steps: combining chitosan oligosaccharide molecules with sepiolite, and combining a composite bacterial liquid with a chitosan oligosaccharide-sepiolite composite matrix to obtain a chitosan oligosaccharide-sepiolite-composite probiotic carrier; sodium sulfite and epichlorohydrin are utilized to enable the modified lignin to have higher solubility, and the modified lignin is combined with the nano montmorillonite to form a stable network through glutaraldehyde; converting the carbon-rich biomass into biochar, and combining starch, soybean protein and a mixed trace element solution to obtain a composite synergist; lignocellulose of agricultural and forestry wastes is subjected to structural damage, and cellulose and hemicellulose are degraded and a carbon source is provided for subsequent functional microorganisms under the synergistic effect of a compound enzyme solution; in the composting fermentation process, deep degradation of organic matters is achieved, a sodium alginate solution is sprayed to form a stable nutrient slow-release network, and meanwhile the water retention capacity of the particles is improved.

The invention discloses a soil remediation and microbiological detection system for agriculture, and relates to the technical field of soil remediation, the system comprises the following components: a straw treatment and microbiological proliferation module, which is used for crushing straws obtained after crops are harvested to 5-10cm and mixing the crushed straws into a position with the depth of 10-20cm on the surface layer of soil; calculating and adding a proper amount of low-temperature cellulose degrading bacteria according to soil and straw conditions, and supplementing nutrient substances to proliferate in the soil; by accurately controlling straw in-situ field returning and microorganism adding, the soil fertility is effectively improved, the soil structure is improved, the content of organic matter and inorganic salt in the soil is increased by means of close combination of low-temperature cellulose degrading bacteria and straw, and by regulating nitrogenous fertilizer operation and applying a decomposition agent in a matched mode, the soil quality is improved. According to the method, the number of soil microorganisms and the population structure are optimized, the soil remediation effect and efficiency are remarkably improved, the problems that soil fertility is reduced and the structure is damaged are solved, and sustainable development of the agricultural ecological environment is further promoted.

The invention discloses an efficient composting and resource utilization method for garden waste. The method comprises the following steps: S1, classification pretreatment: classifying the garden waste into high lignin, medium lignin and low lignin; respectively crushing until the particle size is less than or equal to 5mm (class A) or less than or equal to 3mm; adjusting the initial carbon-nitrogen ratio (C / N) of the mixed material to (35-45): 1 and the initial water content to 55-65%; s2, preparation and inoculation of a complex microbial inoculant: preparing a complex functional microbial inoculant containing a lignin degrading microbial inoculant, a cellulose / hemicellulose degrading microbial inoculant, a function enhancing microbial inoculant and a protective carrier, and inoculating the complex functional microbial inoculant into the pretreated material with the inoculation amount being 0.5-1.0% of the total dry weight of the material. The garden waste is finely classified and crushed according to the lignin content, and the physical structure and the initial C / N of the material are optimized; the white rot fungi, the trichoderma reesei, the bacillus subtilis and other strains in the complex microbial inoculant have a synergistic effect, and particularly, the white rot fungi specifically degrade lignin, so that the degradation bottleneck of a lignocellulose composite structure is effectively broken.

The invention relates to the technical field of microbial fermentation, and discloses a high-protein pig feed prepared by a microbial fermentation technology, which is prepared by the following steps: S1, mixing soybean meal, corn straw and bran according to a mass ratio of 5: 3: 2, crushing to 60-80 meshes, adding a compound microbial agent and water, and adjusting the water content to 45-50% to prepare a fermentation base material; the method comprises the following steps: carrying out first-stage solid-state fermentation for 48-60 hours under the condition of 35-38 DEG C, introducing sterile air in the fermentation process and maintaining the pressure in a tank to be 0.05-0.1 MPa and the stirring speed to be 30-50 rpm, and by arranging a complex microbial inoculant synergistic fermentation system, when the high-protein feed is prepared from soybean meal, corn straws and other plant raw materials, the high-protein feed can be prepared from the soybean meal, the corn straws and other plant raw materials. The specific compatibility proportion of bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae is screened, the synchronous effect of cellulose degradation, organic acid synthesis and mycoprotein enrichment is achieved, and efficient passivation of anti-nutritional factors of different raw materials is guaranteed.

The invention relates to the technical field of microorganisms, in particular to a saline-alkaline-tolerant bacillus velezensis JT6, a cellulose degrading enzyme secreted by the same and application of the saline-alkaline-tolerant bacillus velezensis JT6. The bacillus velezensis JT6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 5, 2025, and the preservation number is CGMCC No.36918. The bacillus velezensis JT6 disclosed by the invention can be used for preparing the bacillus velezensis JT6. The strain has cellulose degradation activity, the degradation rate of corn straw is 48.8%, and the degradation rate of rice straw is 24.92%. The strain provided by the invention is used for rapid decomposition of straw returning, is safe to human and livestock, has no pollution to the environment, and has good development and application prospects.

The present invention discloses Trichoderma asperellum ZC3 and its application in the degradation of Zanthoxylum bungeanum branches. This strain has a fast growth rate and strong degradation ability. In a liquid filter paper strip culture medium, after culturing at 28 °C for 7 days, the filter paper strip completely disintegrates; in a solid culture medium of Zanthoxylum bungeanum branch powder, after culturing at 28 °C for 40 days, its lignin degradation rate for Zanthoxylum bungeanum branches reaches 78.11%, and the degradation rates of cellulose and hemicellulose reach 82.58% and 75.84% respectively. Under a scanning electron microscope, obvious fiber breakage and structural disintegration phenomena can be seen in the Zanthoxylum bungeanum branches, showing a high degradation ability. In addition, the lignin-degrading bacterium ZC3 has good tolerance to the leaching solution of Zanthoxylum bungeanum branches.

The invention relates to the technical field of microorganisms, in particular to the technical field of agricultural microorganisms, and more particularly relates to a cellulose degradation composite flora as well as a construction method and application thereof. The cellulose degradation composite flora comprises a cellulose degradation bacterium, namely humid cellulomonas CW35 and an auxiliary strain used for digesting an intermediate metabolite of the humid cellulomonas CW35, and the auxiliary strain is one or more of pseudomonas Taiwan CW19, bacillus amyloliquefaciens L3 or microbacterium algal M266-2. Therefore, the feedback inhibition effect of a product in the cellulose degradation process of the cellulomonas can be destroyed, the expression and enzyme activity of cellulose degradation related genes are remarkably improved, and the decomposition utilization of cellulose-degrading bacteria, namely the wet cellulomonas CW35, on cellulose-containing wastes is effectively improved.

The invention discloses a method for accelerating weathering and soil formation of waste concrete by microbial fermentation, which comprises the following steps: crushing the waste concrete into blocks with the particle size of 20-50cm, retaining the fracture structure of the blocks, mixing the concrete blocks with biomass, adding 0.3-0.5% of compound microbial agent which specifically comprises acid-producing bacteria, cellulose degrading bacteria and silicate weathering bacteria into the mixed material, and uniformly stirring to obtain a mixture; composting the mixed material, controlling the temperature of the pile body to be 40-55 DEG C, maintaining for at least 15 days, and gradually decomposing the concrete block body by utilizing the synergistic biological weathering effect of organic acid generated by microbial metabolism and the microbial corrosion effect in the fermentation process. According to the method disclosed by the invention, the dynamic bottleneck of decomposition of calcium silicate minerals in concrete blocks is broken through by virtue of synergistic fermentation of the composite flora and the biomass and the coupling effect of organic acid release and biological erosion, and the crushing energy consumption is reduced compared with that of a traditional fine crushing process; and a novel green conversion method integrating rapid soil formation, nutrient activation and pollution prevention and control is formed.

The invention provides a cellulose degrading strain for degrading traditional Chinese medicine residues, a microbial agent and a method for degrading the traditional Chinese medicine residues, and relates to the technical field of microorganisms. The invention provides a cellulose degrading strain for degrading traditional Chinese medicine residues, which comprises Cellulomonas X-5 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.34614, and Cellulomonas X-5 with the preservation number of CGMCC No.34614; the preservation number of the cellulomicrobacterium X-7 is CGMCC (China General Microbiological Culture Collection Center) No.34615 According to the research and verification of the application, both the cellulomonas X-5 and the cellulomicrobacterium X-7 have good fiber degradation activity, especially for multi-medicinal material mixed traditional Chinese medicine residues, the decomposition rate of the traditional Chinese medicine residues can be remarkably improved, and meanwhile, the contents of quick-acting phosphorus and alkali-hydrolyzable nitrogen in the degraded traditional Chinese medicine residues can also be improved. Therefore, the cellulose degrading strain obtained in the invention not only is helpful for realizing resource utilization of agricultural wastes, but also can effectively improve the soil fertility and reduce the risk of environmental pollution.

This invention discloses a strain of *Bacillus parabrachium* ABX1 and its applications, belonging to the field of beneficial microbial technology. The *Bacillus parabrachium* ABX1 provided by this invention is a Gram-positive bacterium isolated from the intestinal fluid of *Bombyx mori*. On CMC-Na solid medium, the ratio of the diameter of the hydrolysis zone to the colony diameter can reach 11. Enzyme activity identification results show that the filter paper enzyme activity of *Bacillus parabrachium* ABX1 is as high as 109.79 U·mL. ‑1 The exo-β-glucanase activity was 107.59 U·mL. ‑1 The cellulase activity reached 107.37 U·mL ‑1 It exhibits excellent cellulose and / or crystalline cellulose degradation capabilities; it is easy to use and has a wide range of enzyme production conditions, showing excellent enzyme activity at pH 5–10 and 25–50℃; the enzyme production time is short, with high enzyme yield achieved in just 12 hours, and there is no significant adverse effect on enzyme yield with prolonged reaction time; it is highly safe to use and has great application prospects in areas such as oak garden waste branch treatment, improvement of oak silkworm digestibility, industrial degradation of cellulose, and improvement of soil fertility.

The invention relates to the technical field of biological sewage treatment, in particular to a method for realizing heterotrophic nitrification-aerobic denitrification nitrogen removal of wastewater with a low carbon nitrogen ratio. According to the method for realizing heterotrophic nitrification-aerobic denitrification nitrogen removal of the wastewater with the low carbon-nitrogen ratio, an exogenous magnetic field is introduced into a slow-release carbon source system, so that the central magnetic field intensity of an aeration tank is 10-40mT, and the metabolic activity of cellulose degrading bacteria in the system is enhanced and the degradation process of cellulose is promoted by utilizing a magnetic biological effect excited by the magnetic field; the carbon source release efficiency is improved, and the problem of limited carbon release amount of the slow-release carbon source is solved. The enhanced carbon release process effectively increases the carbon nitrogen ratio, provides sufficient organic carbon sources and electron donors for heterotrophic nitrification-aerobic denitrification bacteria, makes the heterotrophic nitrification-aerobic denitrification bacteria have advantages in competition with autotrophic bacteria, further enhances the dominant position of heterotrophic nitrification-aerobic denitrification in the denitrification process without changing other operating parameters, realizes efficient denitrification, and improves the denitrification efficiency. Cost is low, operation is simple, and effect is remarkable.

The invention discloses a buffalo rumen source lignin and cellulose degrading bacterium microecological preparation as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The active ingredient of the microecological preparation comprises bacillus sorola desert, and the preservation number of the bacillus sorola desert in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2025512. According to the invention, a strain of bacillus sorola, which has lignin degradation ability and can degrade cellulose, is screened from a rumen solid-liquid mixture sample obtained from a buffalo rumen fistula, and is developed into a microecological preparation. Experimental verification shows that the degradation rate of the strain to sodium lignin sulfonate in 24 hours reaches 9.54%, the degradation rate of the strain to straw in 3 days can reach 8.6% or above, and in an artificial simulation rumen test, lignin in the straw can be remarkably degraded, and meanwhile, the fiber utilization value of the straw is effectively improved.

The invention discloses a stropharia rugoso-annulata culture medium with tobacco stalks as a main material and a culture method and application of the stropharia rugoso-annulata culture medium, and relates to the technical field of edible fungus cultivation, the stropharia rugoso-annulata culture medium is prepared from, by dry weight, 20-40 parts of corn stalks, 5-15 parts of wheat bran, 0-18 parts of cottonseed hulls, 1-2 parts of lime, 0.5-1 part of gypsum and 0.3-0.5 part of microbial detoxification fungicide. And 30-50 parts of tobacco stalks. According to the method, wood chips are replaced by the tobacco stems in a high proportion of 30%-50%, and the growth speed of hyphae can be increased; through aerobic and anaerobic combined fermentation and a microbial detoxification process, the nicotine residue of the substrate can be reduced to 15 mg / kg or below. In addition, the microbial detoxification bacterial agent Bacillus subtilis YN-1T has acid resistance and efficient cellulose degradation capability, and can secrete cellulase to decompose cellulose and hemicellulose in tobacco stems, so that the matrix structure is further optimized, and the hypha planting efficiency is improved.

The invention discloses a straw-based biological fermentation feed and a preparation method thereof. The preparation method comprises the steps of straw raw material pretreatment, fermentation strain preparation and inoculation, fermentation process control, post-treatment and drying. The invention relates to the technical field of feed processing, aiming at the problems of low digestibility and poor fermentation process of straw feed, corn straw and the like are taken as raw materials, and glucose is added for pretreatment after crushing; preparing a bacterial liquid from lactic acid bacteria, saccharomycetes and white rot fungi (3: 2: 1), and inoculating; fermenting for 5-6 days by controlling the temperature, the humidity and the ventilatory capacity in stages, wherein the terminal pH is 4.0-4.5, and the cellulose degradation rate is greater than or equal to 40%; and mixing according to different formulas of pigs and cows after drying and crushing. The method is thorough in crude fiber degradation, free of chemical residues, short in period, high in feed nutrition and safe, straw can be recycled, and the method is suitable for large-scale production.

The invention discloses an embrittled beetle sample and a preparation method thereof, and belongs to the technical field of cultural relic repair and protection. The method comprises the following steps: step 1, selecting palm plant leaves, cutting the palm plant leaves to obtain a beetle sample, soaking the beetle sample in water, heating the beetle sample to remove surface impurities and internal soluble substances, and enabling the beetle sample to be in a saturated water state; 2, preparing a sulfuric acid solution, and soaking the pretreated Betashes in the sulfuric acid solution through a sample to realize different degrees of cellulose degradation and embrittlement effects; and 3, washing the sample with deionized water, repeatedly soaking the sample treated in the step 2 until the sample is neutral, and drying the sample to obtain the embrittled sample. The method has the advantages that the fibers of the beetle are controllably degraded through the sulfuric acid solution, that is, natural embrittlement of the beetle is simulated through chemical treatment, the embrittlement state of the beetle can be quickly simulated, a standardized sample is provided for protection and repair research, and the method can be used for cultural relic protection material testing, repair technology verification and aging mechanism research.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The invention discloses expansin recombinant aspergillus niger suitable for liquid fermentation as well as a construction method and application thereof, and belongs to the technical field of recombinant microorganisms. The technical problem of efficiently degrading cellulose is solved. The invention provides a recombinant aspergillus niger, and the recombinant aspergillus niger is obtained by taking aspergillus niger TH-2-D as a starting strain and replacing a saccharifying enzyme gene glaA as shown in SEQ ID NO.31 with a penicillium oxalicum expansin Swo gene as shown in SEQ ID NO.28. The invention also provides a preparation method of the recombinant aspergillus niger. The strain is subjected to liquid state fermentation, fermentation liquor is suitable for fiber puffing application, the cellulose degradation efficiency can be improved when the strain is used in cooperation with cellulase, linked starch can be effectively removed when the strain is used in cooperation with corn wet grinding enzyme, and the starch yield is increased.

This invention discloses a highly efficient microbial process for preparing liquefied cellulose composite inoculants, relating to the field of microbial acoustic magnetic field treatment. The process includes the following steps: Step 1, inoculum activation and seed culture preparation; Step 2, inoculation and fermentation; Step 3, multi-field coupling enhancement and online detection; Step 4, intelligent feedback control; and Step 5, inoculum post-treatment. The intelligent multi-field coupled reactor includes a tank for carrying the inoculum, an ultrasonic stirring device located within the tank, a magnetic field generating device located on the outer wall of the tank, and online viscometers, fluorescent dissolved oxygen sensors, and near-infrared enzyme activity sensors for detecting the inoculum within the tank. The preparation process provided by this invention, by constructing an intelligent fermentation system with multi-field coupling of ultrasound and magnetic fields, fundamentally solves the technical defects of traditional cellulose-degrading inoculants, such as long fermentation cycles and low enzyme activity.

The invention provides non-sugar-tolerant beta-glucosidase, an expression vector and application of the non-sugar-tolerant beta-glucosidase in improvement of cellulose degradation capacity, and belongs to the technical field of genetic engineering. According to the invention, the non-sugar tolerance type beta-glucosidase BGLC27 is obtained from streptomyces decomposer C27, and the amino acid sequence of the BGLC27 is as shown in SEQ ID No. 1. According to the invention, the BGLC27 is used as an exogenous enzyme and is added into a degradation system of cellulose degradation bacteria, and research finds that after the BLGC27 is exogenously added into the compound bacteria system, the cellulose degradation capability can be obviously improved.

The invention discloses a microbial agent for treating agriculture, forestry, livestock and poultry wastes and a preparation method thereof, the microbial agent is composed of salt-tolerant bacillus, acid-resistant nitrococcus, bacillus amyloliquefaciens and pantoea agglomerans according to a weight part ratio of 3: 2: 1: 0.5, and the microbial agent is subjected to gradient stress-AHLs signal induction domestication. The preparation method comprises the steps of seed culture, synergistic induction culture and microcapsule embedding, active flora synergy is achieved through quorum sensing, and the three-in-one function of degradation, growth promotion and disease resistance is achieved. The microbial agent is high in stress resistance, the shelf life is 12-18 months, the degradation rate of cellulose in 7 days is 65%-75%, the microbial agent is suitable for resource utilization of various agricultural, forestry, livestock and poultry wastes, and a treated product can be directly used as a functional organic fertilizer.

The application belongs to the technical field of microorganisms, and particularly relates to a long oyster parabacillus ZH-28 and application thereof. Metabacillus crassostreae The long oyster parabacillus ZH-28 has been preserved in the China General Microbiological Culture Collection Center on January 27, 2026, and the preservation number is CGMCC No.37550. Metabacillus crassostreae The long oyster parabacillus ZH-28 has strong salt and alkali resistance, and has multiple functions such as nitrogen fixation, protein degradation, cellulose degradation, fat degradation and iron carrier production, and has significant growth promotion on wheat and cucumber in a saline-alkali environment, and has a good application prospect in plant growth promotion in a saline-alkali soil.

The embodiment of the invention provides a screening method and system for cellulose degrading bacteria. The method comprises the following steps: determining all genes of all microorganisms to be screened in a sample to be detected and first gene abundance data of all genes; based on the functional gene set, screening out a plurality of target genes from all the genes; determining second gene abundance data of the plurality of target genes according to the first gene abundance data; determining the cellulose degradation fraction of each microorganism to be screened according to the second gene abundance data; screening out cellulose degrading bacteria in all the microorganisms to be screened according to the cellulose degradation fraction of each microorganism to be screened. Meanwhile, a large number of microorganisms to be screened can be analyzed, and the screening efficiency is improved.