93 results about "Cellulose degradation" patented technology

The invention discloses an efficient composting and resource utilization method for garden waste. The method comprises the following steps: S1, classification pretreatment: classifying the garden waste into high lignin, medium lignin and low lignin; respectively crushing until the particle size is less than or equal to 5mm (class A) or less than or equal to 3mm; adjusting the initial carbon-nitrogen ratio (C / N) of the mixed material to (35-45): 1 and the initial water content to 55-65%; s2, preparation and inoculation of a complex microbial inoculant: preparing a complex functional microbial inoculant containing a lignin degrading microbial inoculant, a cellulose / hemicellulose degrading microbial inoculant, a function enhancing microbial inoculant and a protective carrier, and inoculating the complex functional microbial inoculant into the pretreated material with the inoculation amount being 0.5-1.0% of the total dry weight of the material. The garden waste is finely classified and crushed according to the lignin content, and the physical structure and the initial C / N of the material are optimized; the white rot fungi, the trichoderma reesei, the bacillus subtilis and other strains in the complex microbial inoculant have a synergistic effect, and particularly, the white rot fungi specifically degrade lignin, so that the degradation bottleneck of a lignocellulose composite structure is effectively broken.

The invention relates to the technical field of microorganisms, in particular to a saline-alkaline-tolerant bacillus velezensis JT6, a cellulose degrading enzyme secreted by the same and application of the saline-alkaline-tolerant bacillus velezensis JT6. The bacillus velezensis JT6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 5, 2025, and the preservation number is CGMCC No.36918. The bacillus velezensis JT6 disclosed by the invention can be used for preparing the bacillus velezensis JT6. The strain has cellulose degradation activity, the degradation rate of corn straw is 48.8%, and the degradation rate of rice straw is 24.92%. The strain provided by the invention is used for rapid decomposition of straw returning, is safe to human and livestock, has no pollution to the environment, and has good development and application prospects.

The invention relates to the technical field of microorganisms, in particular to the technical field of agricultural microorganisms, and more particularly relates to a cellulose degradation composite flora as well as a construction method and application thereof. The cellulose degradation composite flora comprises a cellulose degradation bacterium, namely humid cellulomonas CW35 and an auxiliary strain used for digesting an intermediate metabolite of the humid cellulomonas CW35, and the auxiliary strain is one or more of pseudomonas Taiwan CW19, bacillus amyloliquefaciens L3 or microbacterium algal M266-2. Therefore, the feedback inhibition effect of a product in the cellulose degradation process of the cellulomonas can be destroyed, the expression and enzyme activity of cellulose degradation related genes are remarkably improved, and the decomposition utilization of cellulose-degrading bacteria, namely the wet cellulomonas CW35, on cellulose-containing wastes is effectively improved.

The invention provides a cellulose degrading strain for degrading traditional Chinese medicine residues, a microbial agent and a method for degrading the traditional Chinese medicine residues, and relates to the technical field of microorganisms. The invention provides a cellulose degrading strain for degrading traditional Chinese medicine residues, which comprises Cellulomonas X-5 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.34614, and Cellulomonas X-5 with the preservation number of CGMCC No.34614; the preservation number of the cellulomicrobacterium X-7 is CGMCC (China General Microbiological Culture Collection Center) No.34615 According to the research and verification of the application, both the cellulomonas X-5 and the cellulomicrobacterium X-7 have good fiber degradation activity, especially for multi-medicinal material mixed traditional Chinese medicine residues, the decomposition rate of the traditional Chinese medicine residues can be remarkably improved, and meanwhile, the contents of quick-acting phosphorus and alkali-hydrolyzable nitrogen in the degraded traditional Chinese medicine residues can also be improved. Therefore, the cellulose degrading strain obtained in the invention not only is helpful for realizing resource utilization of agricultural wastes, but also can effectively improve the soil fertility and reduce the risk of environmental pollution.

This invention discloses a strain of *Bacillus parabrachium* ABX1 and its applications, belonging to the field of beneficial microbial technology. The *Bacillus parabrachium* ABX1 provided by this invention is a Gram-positive bacterium isolated from the intestinal fluid of *Bombyx mori*. On CMC-Na solid medium, the ratio of the diameter of the hydrolysis zone to the colony diameter can reach 11. Enzyme activity identification results show that the filter paper enzyme activity of *Bacillus parabrachium* ABX1 is as high as 109.79 U·mL. ‑1 The exo-β-glucanase activity was 107.59 U·mL. ‑1 The cellulase activity reached 107.37 U·mL ‑1 It exhibits excellent cellulose and / or crystalline cellulose degradation capabilities; it is easy to use and has a wide range of enzyme production conditions, showing excellent enzyme activity at pH 5–10 and 25–50℃; the enzyme production time is short, with high enzyme yield achieved in just 12 hours, and there is no significant adverse effect on enzyme yield with prolonged reaction time; it is highly safe to use and has great application prospects in areas such as oak garden waste branch treatment, improvement of oak silkworm digestibility, industrial degradation of cellulose, and improvement of soil fertility.

The invention relates to the technical field of biological sewage treatment, in particular to a method for realizing heterotrophic nitrification-aerobic denitrification nitrogen removal of wastewater with a low carbon nitrogen ratio. According to the method for realizing heterotrophic nitrification-aerobic denitrification nitrogen removal of the wastewater with the low carbon-nitrogen ratio, an exogenous magnetic field is introduced into a slow-release carbon source system, so that the central magnetic field intensity of an aeration tank is 10-40mT, and the metabolic activity of cellulose degrading bacteria in the system is enhanced and the degradation process of cellulose is promoted by utilizing a magnetic biological effect excited by the magnetic field; the carbon source release efficiency is improved, and the problem of limited carbon release amount of the slow-release carbon source is solved. The enhanced carbon release process effectively increases the carbon nitrogen ratio, provides sufficient organic carbon sources and electron donors for heterotrophic nitrification-aerobic denitrification bacteria, makes the heterotrophic nitrification-aerobic denitrification bacteria have advantages in competition with autotrophic bacteria, further enhances the dominant position of heterotrophic nitrification-aerobic denitrification in the denitrification process without changing other operating parameters, realizes efficient denitrification, and improves the denitrification efficiency. Cost is low, operation is simple, and effect is remarkable.

The invention discloses a buffalo rumen source lignin and cellulose degrading bacterium microecological preparation as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The active ingredient of the microecological preparation comprises bacillus sorola desert, and the preservation number of the bacillus sorola desert in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2025512. According to the invention, a strain of bacillus sorola, which has lignin degradation ability and can degrade cellulose, is screened from a rumen solid-liquid mixture sample obtained from a buffalo rumen fistula, and is developed into a microecological preparation. Experimental verification shows that the degradation rate of the strain to sodium lignin sulfonate in 24 hours reaches 9.54%, the degradation rate of the strain to straw in 3 days can reach 8.6% or above, and in an artificial simulation rumen test, lignin in the straw can be remarkably degraded, and meanwhile, the fiber utilization value of the straw is effectively improved.

The invention discloses a stropharia rugoso-annulata culture medium with tobacco stalks as a main material and a culture method and application of the stropharia rugoso-annulata culture medium, and relates to the technical field of edible fungus cultivation, the stropharia rugoso-annulata culture medium is prepared from, by dry weight, 20-40 parts of corn stalks, 5-15 parts of wheat bran, 0-18 parts of cottonseed hulls, 1-2 parts of lime, 0.5-1 part of gypsum and 0.3-0.5 part of microbial detoxification fungicide. And 30-50 parts of tobacco stalks. According to the method, wood chips are replaced by the tobacco stems in a high proportion of 30%-50%, and the growth speed of hyphae can be increased; through aerobic and anaerobic combined fermentation and a microbial detoxification process, the nicotine residue of the substrate can be reduced to 15 mg / kg or below. In addition, the microbial detoxification bacterial agent Bacillus subtilis YN-1T has acid resistance and efficient cellulose degradation capability, and can secrete cellulase to decompose cellulose and hemicellulose in tobacco stems, so that the matrix structure is further optimized, and the hypha planting efficiency is improved.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The invention discloses expansin recombinant aspergillus niger suitable for liquid fermentation as well as a construction method and application thereof, and belongs to the technical field of recombinant microorganisms. The technical problem of efficiently degrading cellulose is solved. The invention provides a recombinant aspergillus niger, and the recombinant aspergillus niger is obtained by taking aspergillus niger TH-2-D as a starting strain and replacing a saccharifying enzyme gene glaA as shown in SEQ ID NO.31 with a penicillium oxalicum expansin Swo gene as shown in SEQ ID NO.28. The invention also provides a preparation method of the recombinant aspergillus niger. The strain is subjected to liquid state fermentation, fermentation liquor is suitable for fiber puffing application, the cellulose degradation efficiency can be improved when the strain is used in cooperation with cellulase, linked starch can be effectively removed when the strain is used in cooperation with corn wet grinding enzyme, and the starch yield is increased.

This invention discloses a highly efficient microbial process for preparing liquefied cellulose composite inoculants, relating to the field of microbial acoustic magnetic field treatment. The process includes the following steps: Step 1, inoculum activation and seed culture preparation; Step 2, inoculation and fermentation; Step 3, multi-field coupling enhancement and online detection; Step 4, intelligent feedback control; and Step 5, inoculum post-treatment. The intelligent multi-field coupled reactor includes a tank for carrying the inoculum, an ultrasonic stirring device located within the tank, a magnetic field generating device located on the outer wall of the tank, and online viscometers, fluorescent dissolved oxygen sensors, and near-infrared enzyme activity sensors for detecting the inoculum within the tank. The preparation process provided by this invention, by constructing an intelligent fermentation system with multi-field coupling of ultrasound and magnetic fields, fundamentally solves the technical defects of traditional cellulose-degrading inoculants, such as long fermentation cycles and low enzyme activity.

The invention discloses a microbial agent for treating agriculture, forestry, livestock and poultry wastes and a preparation method thereof, the microbial agent is composed of salt-tolerant bacillus, acid-resistant nitrococcus, bacillus amyloliquefaciens and pantoea agglomerans according to a weight part ratio of 3: 2: 1: 0.5, and the microbial agent is subjected to gradient stress-AHLs signal induction domestication. The preparation method comprises the steps of seed culture, synergistic induction culture and microcapsule embedding, active flora synergy is achieved through quorum sensing, and the three-in-one function of degradation, growth promotion and disease resistance is achieved. The microbial agent is high in stress resistance, the shelf life is 12-18 months, the degradation rate of cellulose in 7 days is 65%-75%, the microbial agent is suitable for resource utilization of various agricultural, forestry, livestock and poultry wastes, and a treated product can be directly used as a functional organic fertilizer.

The application belongs to the technical field of microorganisms, and particularly relates to a long oyster parabacillus ZH-28 and application thereof. Metabacillus crassostreae The long oyster parabacillus ZH-28 has been preserved in the China General Microbiological Culture Collection Center on January 27, 2026, and the preservation number is CGMCC No.37550. Metabacillus crassostreae The long oyster parabacillus ZH-28 has strong salt and alkali resistance, and has multiple functions such as nitrogen fixation, protein degradation, cellulose degradation, fat degradation and iron carrier production, and has significant growth promotion on wheat and cucumber in a saline-alkali environment, and has a good application prospect in plant growth promotion in a saline-alkali soil.

The invention provides a complex microbial inoculant, effervescent granules and application, and belongs to the technical field of microorganisms. The invention provides a complex microbial inoculant. The complex microbial inoculant comprises bacillus halotolerant SSF1 and bacillus licheniformis LU1. The complex microbial inoculant has relatively high enzyme activity of endo-glucosidase and relatively good cellulose degradation capability. The complex microbial inoculant is used as an active component and can be combined with a disintegrating agent, a lubricating agent, an adhesive and the like to prepare effervescent granules. The obtained effervescent granules are short in disintegration time and large in foaming amount, the pH value of the effervescent granules is close to the pH value of pure water after the effervescent granules are completely disintegrated, the viable count of the complex microbial inoculant can be efficiently kept, and the complex microbial inoculant can maintain high activity. The result of the embodiment shows that when the effervescent granules of the complex microbial inoculant are applied to wheat planting, the growth of wheat can be remarkably promoted, the germination number of wheat can be increased, and the overground part length, the underground part length, the fresh weight root-crown ratio and the dry weight root-crown ratio of wheat can be improved.

The invention belongs to the technical field of biological environmental protection, and discloses Pseudoxanthomonas suwangensis NS44, a microbial agent and application of the Pseudoxanthomonas suwangensis NS44, the Pseudoxanthomonas suwangensis NS44 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.35937, the preservation date is September 16, 2025, and the preservation number is CGMCC No.35937. The preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The pseudoxanthomonas suwangensis NS44 has the effects of efficiently degrading cellulose, synthesizing IAA and dissolving organic and inorganic phosphorus. The cellulose degrading bacterial agent prepared by the invention is applied to the process of mixing and composting corn straws and pig manure in different proportions, so that the temperature of a compost body can reach more than 55 DEG C faster and lasts for a long time, the degradation of corn straw cellulose and hemicellulose is remarkably improved, insoluble phosphorus in the compost body is synchronously converted into effective nutrients, and the compost quality is improved.

The invention belongs to the technical field of microorganisms, and particularly relates to a fungus strain capable of efficiently degrading lignocellulose as well as a screening method and application of the fungus strain. The fungus strain is named as QMJ-1, and is classified and named as Fusarium sp. The fungus strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO: 42128, and the fungus strain is preserved on July 15, 2025. The fungus strain for degrading lignocellulose, which is obtained by the screening method disclosed by the invention, has a complete lignocellulose degrading enzyme system, including filter paper enzyme, carboxymethyl cellulase, xylanase, laccase, manganese peroxidase and the like, and various enzymes are high in activity and have the capability of efficiently degrading lignocellulose. In addition, the screening method disclosed by the invention is simple and convenient in steps and relatively low in cost, can effectively convert agricultural wastes into value-added products, and has a good application prospect.

Currently, it is not possible to install sensors within power transformers to directly measure water content in the solid insulation of the windings. As a result, the extent of the degradation of the insulation is typically not known without maintenance intervention. Accordingly, the disclosed embodiments model the thermo-chemical degradation in the digital twin of the transformer based on the water content in the insulation liquid and the load on the transformer to continuously estimate the water content in segments of the insulation. This estimated water content can take into account the diffusion between the insulation liquid and the insulation, as well as the water generated in the insulation material by cellulose degradation. The estimated water content can be used to estimate other parameters, such as the total water content in the transformer, a measure of the aging of the transformer, etc., as well as inform the maintenance schedule of the transformer.

This invention discloses a deep tenderizing process for jujube peel based on vacuum-ultrasound synergistic infiltration and composite enzyme-microwave coupling, belonging to the field of agricultural product deep processing technology. This process achieves deep tenderizing and quality stability through a five-step method: (1) pretreatment controls surface moisture to 15-18%; (2) vacuum-ultrasound infiltration opens the jujube peel channels, penetrating to a depth of 280-320 μm; (3) quaternary composite enzyme coupled with microwave-ultrasound degrades rigid components, with a cellulose degradation rate ≥90%; (4) stepwise enzyme inactivation (preheating + microwave-water bath) ensures enzyme activity residue <0.2%; (5) segmented drying (microwave-ultrasound synergistic) controls moisture to 18-30%. Experiments show that this process reduces jujube peel hardness to 180-200g, maintains vitamin C retention ≥90%, has a total processing time of 80-110 min, and leaves no chemical residue, solving the problems of incomplete tenderizing, significant nutrient loss, and uneven drying in existing technologies.

The present application relates to the technical field of microorganisms, in particular to a strain of Trichoderma reesei SDU1010, spore suspension and application, the Trichoderme reesei strain SDU1010 is preserved in the China General Microbiological Culture Collection Center on April 7, 2026, and the preservation number is CGMCC No. 3.29531.The Trichoderma reesei strain SDU1010 provided in the present application can effectively produce cellulase under acidic conditions with pH value of 2.5-5.5, effectively makes up the defect of low activity of key enzyme components under acidic conditions of existing strains, and improves the synergistic efficiency of cellulose degradation.

This invention discloses a tobacco-derived α-glucan phosphorylase, its mutants, and their applications. Addressing the problems of low catalytic efficiency, poor stability and adaptability, and difficulty in heterologous expression of key enzyme elements in cellulose-to-starch synthesis, this invention utilizes metagenomics and big data analysis of public databases, along with bioinformatics methods, to precisely identify novel key enzyme elements for cellulose degradation and starch synthesis. An α-glucan phosphorylase from tobacco was identified and found to have better enzyme activity and starch synthesis function than the potato-derived PGP. Furthermore, this invention involves a single point mutation of the Cap domain of the α-glucan phosphorylase. The results show that the single point mutant obtained by mutating the tobacco-derived α-glucan phosphorylase with the amino acid sequence shown in SEQ ID No. 1 by the S524H single point mutation has significantly higher enzyme activity and expression level than the wild-type α-glucan phosphorylase.

The invention relates to Aspergillus oryzae MY. The Aspergillus oryzae MY can rapidly and stably grow by using a cheap culture medium, the fermentation period is short, and the degradation efficiency of lignin and cellulose is high. The strain can efficiently degrade straws, and can be used as a substrate for producing alternative proteins for feed; and reducing sugar can be produced by degrading cellulose, so that the method can be industrially applied and has very high economic value and ecological value.

The invention relates to the technical field of biotechnology, and discloses a microbial cellulose straw degradation, separation and screening method which comprises the following steps: performing multi-stage screening on strains, collecting humus soil samples, and performing three-stage screening on the humus soil samples through a cellulose-Congo red double-layer flat plate primary screening, liquid fermentation secondary screening and enzyme activity quantitative fine screening three-stage screening system. The preparation method comprises the following steps: preparing a cellulose degradation bacterial strain, separating to obtain a cellulose degradation bacterial strain, pretreating straws, crushing corn straws until the particle size is 0.5-2mm, removing a lignin barrier by adopting a diluted acid-alkali combined treatment method, and preparing a composite microbial agent; according to the method disclosed by the invention, a three-stage screening system of cellulose-Congo red double-layer flat plate primary screening, liquid fermentation secondary screening and enzyme activity quantitative fine screening is constructed, so that the capture precision of the efficient degradation strain is improved; the composite mutagenesis technology induces the bacterial strain to generate functional mutation, and the specific compounding proportion of the mutant strain and the original bacterial strain is combined to form a composite bacterial agent with a synergistic effect, so that the natural barrier of lignin-cellulose in the straw is broken, and the biodegradation efficiency of the cellulose is enhanced.

The present disclosure relates to a pectin-containing wastewater treatment method and a treatment device, the method comprising: inputting first pectin wastewater into a biological conversion tank, and performing biological conversion treatment on pectin in the first pectin wastewater based on a pectin-cellulose degradation mixed bacteria to obtain second pectin wastewater containing small molecular structure pectin; wherein the pectin-cellulose degradation mixed bacteria is a mixed bacterial population capable of simultaneously degrading pectin and cellulose, and the biological conversion treatment process is used to change the pectin structure without affecting the chemical oxygen demand corresponding to the first pectin wastewater; inputting the second pectin wastewater into a primary or multi-stage anaerobic reactor for anaerobic decomposition treatment to obtain wastewater with reduced organic matter concentration after pectin decomposition and biogas; and the biogas is used for input into a power supply end or an energy storage end for recycling. In the wastewater treatment process, in-situ treatment and relatively sufficient degradation of pectin are achieved, and the treatment load and treatment efficiency are improved.

The invention relates to the technical field of aviation plant protection aids, and discloses an aviation plant protection aid containing natural plant polysaccharides and a preparation method of the aviation plant protection aid. Constructing a dynamic self-repairing system of a laccase catalysis phenol crosslinking component, a peroxidase catalysis polysaccharide coupling component and a cellulose degradation product repairing storage material, and a biodegradation regulation and control system integrating a cellulase-xylanase compound enzyme component, a tea saponin regulation component and a peptone nutrition component; the functions of enhancing leaf surface attachment, dynamic self-repairing and environment-friendly degradation of the auxiliary agent are realized through multiple synergistic effects. The technical problems that a traditional aviation plant protection auxiliary is insufficient in leaf surface adhesive force, damage cannot be automatically repaired, and environment residues are accumulated are solved, stable adhesion of liquid medicine under the severe weather condition, automatic repairing capacity after film damage and complete biodegradation within 30 days after the pesticide effect period is finished are achieved, and the effect is achieved. And the reliability, persistence and environmental friendliness of aviation plant protection are remarkably improved.

The invention relates to a modified cellulase derived polypeptide and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The invention provides a derivative polypeptide of cellulose excision enzyme Cel7A, the cellulose excision enzyme Cel7A is derived from Trichoderma reesei, NCBI Acession No.XM006969162.1, and the amino acid sequence of the cellulose excision enzyme Cel7A is as shown in SEQ ID NO: 1. The invention further provides a preparation method of the derivative polypeptide of the cellulose excision enzyme Cel7A. Wherein the amino acid sequence of the cellulose binding domain CBM is as shown in SEQ ID NO: 2. The derivative polypeptide is a polypeptide of which the amino acid sequence is 70% or more identical to that of a polypeptide shown in SEQ ID NO: 2, can reduce combination with lignin and improve the degradation efficiency of cellulose, so that the saccharification efficiency of cellulase is improved, the post-treatment process cost is greatly saved, and the derivative polypeptide is suitable for industrial production. The method has important industrial application value.

It is not currently feasible to place a sensor within an electrical transformer to directly measure the water content in the solid insulation of the windings. Thus, the degree of degradation of the insulation can generally not be known without a maintenance intervention. Accordingly, disclosed embodiments model thermochemical degradation in a digital twin of the transformer to continuously estimate the water content in sections of the insulation, based on water content in the insulating liquid and the loading on the transformer. This estimated water content may account for diffusion between the insulating liquid and the insulation, as well as the water generated by cellulose degradation in the insulation material. The estimated water content may be used to estimate other parameters, such as the total water content in the transformer, a measure of aging of the transformer, and / or the like, as well as inform maintenance planning for the transformer.

The invention discloses a full-quality utilization method for continuously preparing biogas from straw biomass, and belongs to the technical field of agricultural waste resource utilization. The method comprises the following steps: firstly, cooperatively treating a lignocellulose raw material by adopting physical crushing and chemical catalysis, and establishing a hydrolysis kinetic equation for monitoring the degradation efficiency of lignocellulose; then transferring the pretreated material into an acidification reaction kettle, inoculating an acid-producing microbial agent, monitoring the VFA concentration, and regulating and controlling the VFA concentration through a reflux ratio R and stirring strength I; and finally, carrying out solid-liquid separation on the obtained material, enabling filtrate to sequentially flow into a multi-stage serial gasification tank, and regulating and controlling the specific growth rate of microorganisms. Through a continuous circulation process, all-component and all-quality utilization of biomass and collaborative prevention and control of pollutants are achieved, a sustainable process system which is short in reaction time, high in system stability, efficient in resource circulation and low in environmental load is finally formed, and the development requirements of precision and low carbon of agricultural waste resource utilization under the dual-carbon target are met.

The present application belongs to the technical field of microorganisms and their applications, and in particular relates to a low-temperature-resistant composite microbial inoculum capable of degrading lignin and cellulose and application of the microbial inoculum in fecal fermentation. The microbial inoculum contains fusiform lysine bacillus LFS1 and bacillus tropicus BTS1. The microbial inoculum was preserved in the China General Microbiological Culture Collection Center on October 24, 2024, and the preservation numbers are GCMCC No: 32336 and No: 32338. The microbial inoculum can accelerate the fecal fermentation process under low-temperature conditions and is helpful to enrich the low-temperature-resistant strain resource library with lignin and cellulose degradation capability. The present application mixes two purified strains in different proportions to prepare a bacterial suspension and develops a series of high-efficiency composite microbial inoculums, which can effectively degrade lignin and cellulose under low-temperature conditions, promote the start-up temperature of composting, and improve the quality of composting. Compared with traditional methods such as artificial heating, the method has higher efficiency, lower cost, and is easier to operate.

The invention discloses a composite microbial inoculant capable of efficiently degrading sugarcane leaves to produce humus and application of the composite microbial inoculant, and belongs to the technical field of microbial degradation. The composite microbial inoculant for efficiently degrading the sugarcane leaves to produce the humus is prepared from a lignin degrading bacterium (Sporobomyces phaffii) X-3, a cellulose degrading bacterium (Tetradium sp.) Lignocellulan-6 and a phosphorus solubilizing fungus (Trichoderma reesei SIP-2, and the composite microbial inoculant for efficiently degrading the sugarcane leaves to produce the humus is prepared from the following raw materials: the lignin degrading bacterium (Sporobomyces phaffii) X-3, the cellulose degrading bacterium (Tetradium sp.) Lignocellulan-6 and the phosphorus solubilizing fungus (Trichoderma reesei SIP-2; aiming at the soil characteristics of the strong acid red soil and the resource utilization requirement of the sugarcane leaf waste, the functional strains adaptive to the acid environment are screened and optimally combined, so that the rapid decomposition of the sugarcane leaves and the efficient generation of the humus are realized, meanwhile, the contents of rapidly available phosphorus and rapidly available potassium in the soil are remarkably increased, and the pH of the soil is improved; the complex microbial inoculant is simple in preparation process and remarkable in application effect, can effectively relieve the problem of degeneration of a southern red soil ecological system, provides technical support for green development of the sugarcane industry, and has wide application prospects.

The invention relates to the technical field of biology, in particular to a zymomonas mobilis recombinant strain for producing ethanol by taking cellobiose as a raw material and application of the zymomonas mobilis recombinant strain. On the basis of recombinant zymomonas mobilis expressing beta-glucosidase, an efficient cellobiose conversion system is constructed through the synergistic effect of promoter and signal peptide optimization and gene multi-copy regulation, the activity of extracellular beta-glucosidase is remarkably improved, cellobiose is accelerated to be hydrolyzed into glucose, and the activity of beta-glucosidase is improved. The yield of ethanol is promoted to be obviously improved. Based on the fact that no linear positive correlation exists between the activity of beta-glucosidase and the yield of ethanol, the bottleneck of cellulose degradation under high substrate concentration is solved through multi-element coordinated regulation and dynamic matching of enzyme activity and substrate metabolism, and the energy efficiency and industrial application value of biomass energy conversion are remarkably improved.