Bacillus licheniformis and application thereof in promotion of cellulose degradation

A technology of bacillus licheniformis and cellulose degrading enzymes, applied in the field of bacillus licheniformis and its application in promoting cellulose degradation, to achieve the effects of promoting compost maturity, improving compost quality, and reducing energy consumption

Inactive Publication Date: 2010-11-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports at home and abroad on the inoculation of cellulolytic bacteria in the high tem...

Method used

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  • Bacillus licheniformis and application thereof in promotion of cellulose degradation
  • Bacillus licheniformis and application thereof in promotion of cellulose degradation
  • Bacillus licheniformis and application thereof in promotion of cellulose degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1, Isolation and Identification of Cellulolytic Bacteria J15

[0026] 1. Isolation of cellulolytic bacteria J15

[0027] Take 5g of compost samples in the high temperature period (60°C-65°C) and add them to 100mL PCS medium, and culture them statically at 60°C. When the filter paper strip was degraded, the original strain was inoculated into fresh PCS medium in an amount of 10% (volume percentage content) to perform primary screening of cellulolytic bacteria. Bacteria with good degradation effects were selected for re-screening. The multiplied culture solution was diluted in multiples and inoculated into the cellulosic bacteria culture medium, and the culture medium of the blank tube was used as a control, and the strains were separated from the sample tube with a good degradation effect of the filter paper strip. When the bacterial species degrades the filter paper strips for a stable time, the culture solution will be diluted with a dilution rate of 10 -1 、...

Embodiment 2

[0040] Example 2, the enzyme production effect of cellulolytic bacteria J15 under different carbon sources

[0041] Three replicates were set up for each medium, and each enzyme activity assay was repeated three times, and the results were averaged.

[0042] 1. Preparation of medium with various carbon sources

[0043] Prepare media for various carbon sources: NaCl 0.5%, CaCO 3 0.2%, carbon source 0.5%, peptone 0.5%, yeast powder 0.1%, distilled water 100mL; pH7.0. The carbon sources used were carboxymethylcellulose (CMC), bran, absorbent cotton, filter paper, glucose or sucrose, respectively.

[0044] 2. Preparation of crude enzyme solution

[0045] The cellulolytic bacteria J15 were inoculated on culture media of various carbon sources (OD600=8.0), cultured at 55° C. for 7 days, and the culture liquid was centrifuged at 8000 r / min for 20 min to obtain crude enzyme liquid.

[0046] 3. Determination of Enzyme Activity

[0047] 1. Detection method of various enzyme activit...

Embodiment 3

[0073] Example 3, the enzyme production effect of cellulolytic bacteria J15 at different temperatures

[0074] Cellulolytic bacteria J15 was inoculated in the PCS medium (OD600=8.0), cultured at different temperatures (45°C, 50°C, 55°C, 60°C, 65°C or 70°C) for 7 days, and the culture liquid Centrifuge at 8000r / min for 20min to obtain crude enzyme solution.

[0075] Detect various enzyme activities of the crude enzyme liquid, the method is the same as step three of embodiment 2. Each temperature setting was repeated three times, and each enzyme activity determination was repeated three times, and the results were averaged.

[0076] see results Figure 4 and Table 3.

[0077] Enzyme activity (U) of the crude enzyme liquid measured under various temperature culture conditions of table 3

[0078]

[0079] The optimum temperature for enzyme production by cellulolytic bacteria J15 was 55-60°C. The culture temperature is in the range of 45-60°C, and the enzyme production abil...

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Abstract

The invention discloses bacillus licheniformis and application thereof in the promotion of cellulose degradation. The invention provides the bacillus licheniformis J15CGMCC No.3905. Fermentation liquor obtained by fermenting the bacillus licheniformis J15 is the cellulose degradation enzymic preparation of the invention. The bacillus licheniformis J15 can be used for degrading cellulose and preparing compost. By degrading straws with cellulose decomposition bacteria, the bacillus licheniformis has the advantages of improving the nutritive value and the utilization value of the straw, along with high nutritive value, reproducibility, environmental friendliness, less energy consumption and the like. By inoculating the cellulose decomposition bacteria to the high temperature compost, cellulase content in the composting process can be obviously increased, the degradation of the cellulose and the decomposition of the compost can be promoted and the quality of the compost can be improved.

Description

technical field [0001] The invention relates to a bacillus licheniformis and its application in promoting cellulose degradation. Background technique [0002] my country is a large agricultural country, and the straw produced every year is more than 600 million tons. How to deal with crop straw is a problem faced by most agricultural areas. If a large amount of waste straw is not disposed of in time, it will not only affect normal farming, but also become a breeding ground for diseases and insect pests. place. As the unit yield of straw crops increases, the total amount of straw increases rapidly, and the phenomenon of straw burning occurs in most areas. Large-scale burning of straw not only causes great environmental pollution, but also wastes precious biological resources. See Table 1 for the total amount of straw of major crops in all provinces and autonomous regions of the country. [0003] Table 1 The total amount of main crop straw in each province (10,000 tons) [0...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C12R1/10
CPCY02W30/40
Inventor 孙振钧李晶
Owner CHINA AGRI UNIV
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