807 results about "Bacillus licheniformis" patented technology

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention provides a southern red soil dry land deacidification soil conditioner and a preparation method thereof, and belongs to the technical field of soil conditioners. The preparation method comprises the following steps: embedding bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens to prepare bacterial microspheres, adding the bacterial microspheres into gel formed by carboxymethyl chitosan and silk fibroin peptide to prepare sustained-release bacterial microsphere gel, uniformly mixing the sustained-release bacterial microsphere gel with porous silicon dioxide loaded with basalt and charcoal, and drying to prepare the acid-reducing soil conditioner for the southern red soil dry land. The acid-reducing soil conditioner for the southern red soil dry land not only improves the pH value of the soil, but also can loosen the soil and improve the air permeability of the soil to a certain extent, and promotes the absorption of crops to moisture and nutrient elements by improving the physical and chemical properties of the soil, so that the normal growth of the crops is ensured, and the acid-reducing soil conditioner has a wide application prospect.

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention discloses a decomposition preparation containing bacillus licheniformis as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The decomposition preparation provided by the invention comprises bacillus licheniformis and Thermus arcuatus. The bacillus licheniformis is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 27, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from coastal saline-alkali soil, has high temperature resistance and salt resistance, and can secrete protease and lipase at the same time. After the bacillus licheniformis and the Thermus arcuatus are mixed, the synergistic effect is remarkable, the duck-derived red mud is effectively promoted to be converted into humic acid, and high-value resource utilization of the duck-derived red mud is achieved.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

The invention discloses a method for increasing the content of pyrazines and 2, 3-butanediol substances through high-temperature Daqu micro-ecological regulation and control, and belongs to the technical field of microbial food processing. The invention discloses a method for improving the content of pyrazines and 2, 3-butanediol substances through micro-ecological regulation and control of high-temperature yeast for making hard liquor, and the method comprises the following steps of: combining and inoculating strains, namely bacillus subtilis CGMCC (China General Microbiological Culture Collection Center) 10830, bacillus velezensis CGMCC 7044 and bacillus licheniformis CGMCC 20675, into wheat flour according to a proportion to prepare the high-temperature yeast for making hard liquor. According to the invention, not only are the contents of pyrazine substances and 2, 3-butanediol increased, but also the abundance of beneficial microorganisms such as bacillus, thermophilic fungi and the like is remarkably improved, and a good saccharification fermentation agent is provided for brewing high-quality white spirit.

According to the method, bacillus licheniformis bacterial liquid and calcium ion liquid are sequentially, slowly and evenly sprayed on the surface of a soil body, soil particles on the surface of a slope are cemented, a layer of calcium carbonate protection film is formed on the surface of the soil body, the anti-scouring capacity of the soil body can be effectively improved, flowing of the soil particles is reduced, and plant growth is protected; the adopted bacillus licheniformis can also increase the number of effective microbial communities in soil, improve the root system environment, promote plant growth, inhibit reproduction of harmful microorganisms, improve the granular structure of the soil, improve the soil, regulate the pH value of the soil, improve the water storage, energy storage and heat preservation of the soil, improve the disease resistance of crops and promote the maturity of plants; after vegetation grows, the effect of water and soil conservation can be improved, generated calcium carbonate can also bond soil particles, water and soil loss is reduced, bacillus licheniformis plays a dual role, multiple utilization of resources is achieved, and wide application prospects are achieved.

The invention discloses an organic fertilizer for planting local cereal crops in a plateau region and a preparation method thereof, and belongs to the technical field of agricultural planting. The organic fertilizer for planting local cereal crops in the plateau region is prepared from the following preparation raw materials: decomposed animal waste, vermiculite, humic acid, citric acid, fish meal, bone meal, straw, cake meal and a compound microbial agent, the carrier is prepared from decomposed animal waste, vermiculite, humic acid and citric acid, so that the mechanical strength and durability of the carrier can be improved, and a good physical state can be kept in the storage, transportation and use processes; through compound use of bacillus licheniformis, bacillus subtilis and bacillus laterosporus and adjustment of the dosage ratio of all strains and the viable count of the compound microbial agent, the soil micro-ecological environment can be improved, breeding of beneficial microorganisms in soil is promoted, growth of harmful bacteria is inhibited, and the soil fertility and the water and fertilizer retention capacity are improved; the fertilizer has a good promotion effect on the growth of spring wheat, oat and other plateau cereal crops.

The invention relates to a decomposition-promoting phosphorus-dissolving deodorizing microbial agent for composting and a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, paenibacillus zerzeri C-A and bacillus chestnut brown SC in a mass ratio of 1.5: (1-3): (1.5-4). The viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g. The preparation method comprises the following steps: (1) strain activation; (2) strain amplification culture; (3) bacterial powder preparation; and (4) strain compounding. The invention relates to application in clean composting of organic wastes. The invention has the following advantages: the three strains in the microbial inoculum do not generate antagonism, and can obviously accelerate the conversion of insoluble phosphorus, promote decomposition and improve the effect of removing NH3 and H2S.

The invention belongs to the field of microorganisms, and particularly relates to a feed containing bacillus paralicheniformis carrying quorum sensing related genes and a preparation method of the feed. The bacillus parlicheniformis is bacillus parlicheniformis Shannan.BP-803, and the bacillus parlicheniformis is bacillus parlicheniformis Shannan.BP-803. The invention also discloses a preparation method of the bacillus parlicheniformis. The viable count of the bacillus paralicheniformis contained in each kg of the feed is 2.1 * 10 < 9 >-7.3 * 10 < 9 > CFU. The bacillus paralicheniformis Shann.BP-803 strain used in the feed disclosed by the invention has the capability of efficiently inhibiting pathogenic bacteria and pathogenic fungi and the capability of degrading mycotoxin, so that after the feed containing the bacillus paralicheniformis Shann.BP-803 strain is eaten, the diarrhea rate of animals is reduced, and the feeding effect is better.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a coated slow-release solid water-soluble fertilizer as well as a preparation method and application thereof. The coated slow-release solid water-soluble fertilizer comprises the following components in parts by weight: 15-20 parts of a nitrogen source, 8-12 parts of a phosphorus source, 15-25 parts of a potassium source, 3-8 parts of humic acid and 10-15 parts of charcoal powder, the composite material comprises the following components in parts by weight: 0.5-1.5 parts of functional microorganisms, 1-2 parts of microbial metabolite, 2-5 parts of seaweed extract and 5-8 parts of chitosan-gelatin composite material. The functional microorganisms are bacillus subtilis, bacillus licheniformis and azospirillum which are adsorbed on the charcoal powder. The coated slow-release solid water-soluble fertilizer provided by the invention has the effects of supplying nutrients and preventing and treating soil-borne diseases, achieves multiple effects by one fertilizer, and can improve the crop nutrient utilization rate, inhibit the soil-borne diseases and promote the diversity of soil microorganisms.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention provides an environment-friendly microbial strain and a water treatment agent preparation process, and relates to the field of water treatment. The environment-friendly microbial strain is prepared from the following raw materials in parts by weight: 20 parts of nitrifying bacteria, 20 parts of denitrifying bacteria, 35 parts of bacillus and 25 parts of EM bacteria, the nitrifying bacteria comprise nitrosomas and nitrobacteria, and the denitrifying bacteria comprise nitrosomas and nitrobacteria. The denitrifying bacteria comprise denitrifying bacilli and sternobacter, the bacillus comprises bacillus subtilis and bacillus licheniformis, and the EM bacteria comprise rhodopseudomonas, lactococcus lactis and saccharomyces cerevisiae. Through flora screening and culture, the culture efficiency is improved, through a fermentation process, fermentation liquor overflow and insufficient dissolved oxygen caused by excessive foam are effectively prevented, the stability of the fermentation process is ensured, through preparation preparation, a good packaging material and a sealing technology, moisture absorption and pollution of a microbial inoculum are prevented, and the quality and safety of a product are ensured.

The invention discloses an extreme thermophilic charcoal complex microbial inoculant and application thereof in aerobic composting of pig manure, and belongs to the technical field of microorganisms. The invention firstly discloses a strain of thermophilic thermophilic bacteria N1209, and secondly discloses an extreme thermophilic biochar complex microbial inoculant, which comprises geobacillus thermoglucosidase BGSC 95A1, bacillus licheniformis ZR-1, the thermophilic thermophilic thermophilic bacteria N1209 and geobacillus stearothermophilus L5. The extremely thermophilic biochar complex microbial inoculant has the characteristic of high temperature resistance, compared with a single microbial inoculant, produced amylase, protease and lipase are higher in activity and show synergistic advantages, decomposition of pig manure can be remarkably promoted, the extremely thermophilic biochar complex microbial inoculant has advantages in the aspects of nitrogen element retention and CH4 and N2O emission reduction in the pig manure composting process, and the extremely thermophilic biochar complex microbial inoculant can be applied to the field of pig manure composting. And a new method is provided for treatment of livestock breeding wastes.

According to the invention, a glycosyl transferase mutant derived from Bacillus licheniformis ZSP01 is used as a research object, AlphaFold 2 is used for constructing a three-dimensional structure of a protein, then technologies such as molecular docking and molecular dynamics simulation are used for analyzing amino acids far away from a catalytic center in glycosyl transferase, site-directed mutagenesis is carried out on the amino acids, and the glycosyl transferase is obtained. The activity of the mutant for catalytically synthesizing the icariside D2 glycosyltransferase is improved.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention belongs to the technical field of bioengineering, and particularly relates to an activity-improved tyrosinase mutant obtained by site-directed mutagenesis through an overlapping PCR technology, and preparation and application thereof. The tyrosinase mutant is obtained by mutation containing F337I / F352I on the basis of wild type tyrosinase as shown in SEQ ID NO.1, the enzyme activity of the tyrosinase mutant is obviously improved compared with that of the wild type tyrosinase, the F337I / F352I mutant gene is used for constructing a recombinant vector, and the recombinant vector is successfully expressed in bacillus subtilis WB600, bacillus amyloliquefaciens CGMCC No.11218 and bacillus licheniformis 2709, so that the tyrosinase mutant is obtained. The recombinant strain with improved enzyme production activity is further optimized through a fermentation process to obtain the novel tyrosinase.

The invention relates to a composition containing fermented peony seed oil and application of the composition in skin care products, and belongs to the technical field of skin care products, the composition is composed of 20-25 wt% of fermented peony seed oil, 2-4.5 wt% of a saw palmetto fruit extract, 4 wt% of a bitter melon fruit extract, 1.5 wt% of an abelmoschus esculentus fruit extract, 0.5-1 wt% of an emulsifier and the balance of deionized water, the fermented peony seed oil is prepared through a fermentation process, and fermentation strains comprise lactobacillus plantarum and bacillus licheniformis. The composition containing fermented peony seed oil provided by the invention does not contain chemical components with high irritation or high skin burden, is mild and non-irritant, can effectively improve eczema, has a remarkable effect, and is suitable for being used as an active component to be added into a skin care product.

According to the invention, glycosyl transferase UGTBL1 derived from Bacillus licheniformis ZSP01 is taken as a research object, AlphaFold 2 is used for modeling, molecular docking, molecular dynamics simulation and other technologies, amino acid in an active pocket region of glycosyl transferase is analyzed, amino acid in a substrate channel is analyzed by software CAVER3.0, site-directed mutagenesis is carried out, and the glycosyl transferase UGTBL1 is obtained. The regioselectivity and the activity of the mutant for catalytically synthesizing the icariside D2 glycosyltransferase are improved.

The invention discloses a novel microbial sludge reduction inoculant and a preparation method and application thereof, and belongs to the technical field of microorganisms, the microbial sludge reduction inoculant comprises a composite inoculant and a biomass additive; the complex microbial inoculant is prepared from the following components in parts by weight: 10 to 12 parts of lactic acid bacteria, 18 to 22 parts of saccharomycetes, 6 to 8 parts of bacillus licheniformis, 12 to 16 parts of bacillus subtilis, 4 to 6 parts of bacillus amyloliquefaciens, 7 to 9 parts of methane sarcina and 3 to 5 parts of horsehair methane bacteria; the biomass additive is fruit peel waste. According to the method disclosed by the invention, a biodegradation system with high activity of various required enzymes can be formed by utilizing the mutual action of microbial flora, dead or aged thalli in the activated sludge can be effectively destroyed and decomposed, meanwhile, organic matters can be decomposed, the sludge is subjected to reduction treatment under the condition that the effluent quality is not influenced, the treatment cost is low, and the treatment effect is good. The method is green and pollution-free and worthy of popularization.

The invention provides a high-activity probiotic food for pets, which is characterized by comprising the following components in parts by weight: 88-93 parts of animal meat source, 8-12 parts of boneless chicken powder, 8-12 parts of fish protein powder, 10-15 parts of potato powder, 3-6 parts of beer yeast powder, 3-6 parts of compound probiotics, 0.8-1.2 parts of metabiotics and 3-6 parts of semen plantaginis. Wherein the compound probiotics are prepared by mixing bacillus subtilis SCS-06, the preservation number of which is CCTCC (China Center for Type Culture Collection) NO: M20211498 and bacillus licheniformis XCL-09, the preservation number of which is CCTCC NO: M20211497. The prepared probiotic grain contains rich nutrient substances, probiotics and traditional Chinese medicine active ingredients, the content of active bacteria in the product is high, and the probiotic grain has a remarkable enhancing effect on digestion promoting ability and immunity of intestinal tracts and is effectively proliferated to continuously protect the intestinal tracts.

The invention discloses a microbial compound feed for improving disease resistance of blue crabs and a preparation method of the microbial compound feed, and belongs to the technical field of feed preparation. The microbial compound feed comprises the following raw materials in parts by weight: 30-40 parts of fermented soybean meal, 20-25 parts of bran, 15-20 parts of fish meal, 5-8 parts of a compound microbial agent, 3-5 parts of algal polysaccharides, 2-3 parts of a radix astragali extract, 1-2 parts of chitosan, 2-3 parts of a mineral premix and 1-1.5 parts of compound vitamins, wherein the compound microbial agent comprises bacillus licheniformis, lactobacillus acidophilus and saccharomyces cerevisiae. The microbial compound feed disclosed by the invention comprehensively improves the disease resistance of the blue crabs and improves the survival rate through various ways, such as nutrition balance, immune regulation and intestinal health improvement, so that the breeding cost is reduced, and the income of farmers is increased.

This invention relates to the field of chicken gizzard lining processing technology, and particularly to an active polypeptide of chicken gizzard lining, its preparation method, and functional products. The preparation method includes the following steps: mixing chicken gizzard lining powder with water and subjecting it to alkaline heat treatment to form a slurry; sequentially adding Bacillus licheniformis alkaline protease, trypsin, and pepsin to the slurry for three separate hydrolysis processes to obtain a hydrolysate; after enzyme inactivation treatment of the hydrolysate, adding Lactobacillus plantarum for fermentation to obtain a fermentation broth; sterilizing the fermentation broth and then filtering it to obtain a fermentation supernatant; sequentially subjecting the fermentation supernatant to membrane filtration, sterilization, and spray drying to obtain the active polypeptide of chicken gizzard lining. The active polypeptide of chicken gizzard lining prepared by the method of this invention exhibits alcohol dehydrogenase activating activity, AGEs inhibitory activity, albumin denaturation inhibitory activity, lipoxygenase inhibitory activity, pancreatic lipase inhibitory activity, and a beneficial bacteria proliferation promoting effect.

The present invention relates to the field of traditional Chinese medicine, and relates to a dandelion compound peptide, a preparation method and uses thereof, comprising the following steps: (1) taking dry dandelion products, pulverizing, adding water, and performing steaming to obtain a liquid mixture; (2) then adding cellulase to the liquid mixture, maintaining the temperature, and stirring for enzymatic hydrolysis; then lowering the temperature, adding neutral protease, maintaining the temperature, and stirring for enzymatic hydrolysis; raising the temperature to inactivate the enzyme to obtain an enzymatic hydrolysate; (3) cooling the enzymatic hydrolysate, adding Bacillus licheniformis for fermentation, maintaining the temperature, and stirring for fermentation, and then raising the temperature for sterilization to obtain a fermentation broth; the present invention can extract active substances from metabolites of traditional Chinese medicine active substances, and the product is non-toxic.

According to the present invention, glycosyl transferase UGTBL1 derived from Bacillus licheniformis ZSP01 is adopted as a research object, AlphaFold 2 is adopted to carry out modeling, molecular docking, molecular dynamics simulation and other technologies, amino acids in an active pocket region of glycosyl transferase are analyzed, site-directed mutagenesis is performed on the amino acids to obtain a series of glycosyl transferase mutants, and salidroside can be highly selectively catalyzed and synthesized.