3235 results about "Bacillus licheniformis" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and / or isobutyric acid (as part of the catabolism of valine and / or isoleucine), 3) butanol and / or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and / or 5) cadaverine and / or putrescine (as parts of the catabolism of lysine and / or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention relates to an inorganic-organic microbial compound fertilizer and a preparation method thereof. The inorganic-organic microbial compound fertilizer is characterized by comprising ordinary high-concentration three-element compound fertilizer, humic acid, fly ash, microbial compound bacteria powder and other elements, wherein the microbial compound bacterial powder is obtained by mixing bacterial powders which are prepared by respectively fermenting and culturing photosynthetic bacteria, yeast, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, bacilluslaterosporus, bacillus megaterium, bacillus mucilaginosus, azotbacteria chroococum and actinomycetes. In the inorganic-organic microbial compound fertilizer, the microbial content of the yeast is no less than 2.0*108cfu / g, and the microbial contents of the other strains are all no less than 2.0*109cfu / g. The inorganic-organic microbial compound fertilizer can centralize the advantages of an organic fertilizer, an inorganic fertilizer and microorganisms, thereby enhancing the stress resistance and the disease resistance of crops, enhancing the fertilizer absorption ability of the crops, lowering the soil hardening degree and improving the soil ecological environment and the quality of the crops.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.

The invention discloses a functional microbial fertilizer and a production method thereof. The production method comprises the following steps: grinding 60%-70% of mushroom residue and 15%-25% of livestock manure to 2-3mm to obtain the major material by a grinder, regulating water content to be 60%-65%; adding composite fermenting agent consisting of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride in accordance with 50g-100g / T; stacking, fermenting and maturing for 15-30d, and turning 5-10 times during the process; evenly mixing the major material prepared from fermentation with auxiliary materials consisting of 6%-10% humic acid and 6%-10% rice hull; adding arbuscular mycorrhiza agent in accordance with 50g-100g / T; and sieving with a 3mm sieve to obtain the fertilizer. The invention provides an economic and effective technological approach for the resource recycling of agricultural wastes in China, and has important application prospect on promoting the development of green agriculture, controlling and reducing rural environmental pollution, improving rural eco-environment and other aspects.

The invention discloses a special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing one or a plurality of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaerieus, clostridium sporogenes, clostridium acetobutylicum, clostridium barati, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichodermareesei and aspergillus niger. Simultaneously, by taking the special microorganism composite bacterial agent as a vaccination bacterial agent, the invention provides an application method for decomposing the crops straws and accelerating the ferment to generate marsh gas; and the microorganism composite bacterial agent-treated crops straws have fast ferment speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no faeces of poultry, and have very wide application range.

The invention provides a preparation method for feed without antibiotics. Feed without antibiotics is complete pig feed without antibiotics, which is prepared from alkali protease and fermenting mixing feed of wholesome funguses and contains the wholesome funguses, a complex enzyme, complex organic acid and other microbe metabolites. The preparation method comprises the preparation steps: weighingthe raw materials of the fermenting feed: corn flour, bean dregs, fermenting bean dregs, fish meal and whey mist which are uniformly mixed; weighing immune polysaccharide, edible salt, calcium hydrophosphate, calcium carbonate, lysine, methionine, various vitamins and various minerals which are uniformly mixed; preparing wine making yeast, alkyl producing pseudofilament yeast, lactobacillus acidophilus, bacillus subtilis and bacillus licheniformis which are uniformly mixed; uniformly mixing all the raw materials and the funguses; adding water to the feed; bagging; sealing; fermenting until nogas is generated; discharging air; sealing again; sealing an outer packing bag and labeling to obtain a finished product. The feed without antibiotics prepared by the method has the advantages of good mouth feel, high nutritious value, easy assimilation and wide market prospect.

A low-temp nitrating bacteria preparation for removing NH3-N and COD from the seawater used in marine farm is prepared from Nitrobacter hamburgensis (AS 1.2729), Nitrobacter winogradskyi (AS 1.2730), Nitrobacter vulgaris (AS 1.2775), Bacillus cereus (AS 1.230), Bacillus licheniformis (AS 1.518) and saccharomyces cerevisiae (AS 2.412) through culturing, and proportional mixing.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu / mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu / mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu / mL(or cfu / g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

The invention discloses a fermented feed additive, a preparation method and application thereof. The fermented feed additive is prepared by fermenting mixed strains and mixed auxiliary materials consisting of monosodium glutamate protein, bean pulp, bran, cottonseed meal, corn fibers, rice bran and powdered rice hulls, wherein the mixed strains are obtained by mixing activated strains of lactobacillus acidophilus, lactobacillus planetarium, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis and bacillus licheniformis in a proportion of (1-5): (1-5): (1-5): (1-5): (1-5): (1-5); and the dosage of the fermented feed additive is 5 to 100 percent of the weight of the animal feed when used for the animal feed. According to the fermented feed additive, the bean pulp, the bran, the cottonseed meal and other raw auxiliary materials are sufficiently utilized, so that the food consumption in the animal feeding process is reduced; the fermented feed additive can be used to partiallyor completely replace the animal feed, and antibiotic medicaments can be reduced or prevented from being used; and no medicament is left, so that the feeding cost of farmers is reduced.

The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.

The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which relates to a biological flocculant. The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which has high flocculation activity, low cost for raw materials and great industrial application potential. A microorganism is Bacillus licheniformis. The method comprises the following steps that: lawn on a fresh inclined plane is transferred to a seed culture medium for culture and then transferred to a fermentation culture medium for culture so as to obtain biological-flocculant fermentation broth; the biological-flocculant fermentation broth is centrifuged to remove precipitate and collect supernatant; the supernatant is added with ethanol, kept to stand, centrifuged and then removed; the precipitate is added with absolute ethanol, stirred and centrifuged to remove the supernatant; ethanol precipitate is added with hexadecyl trimethyl ammonium bromide and centrifuged to remove the supernatant so as to obtain the precipitate; the precipitate is dissolved in a NaCl solution, added with the absolute ethanol, stirred, stood and centrifuged to remove the supernatant so as to obtain the precipitate; and the precipitate is frozen and dried in vacuum so as to obtain pure biological flocculant.

The invention relates to a complex microbial inoculum 707 and a preparation method and application thereof, and belongs to the technical field of biological materials of the application of plant biological regulators to crop planting. The preparation method of the complex microbial inoculum 707 comprises the following step of: performing the cultivation of one-level strains and second-level and third-level fermentation on strains comprising 45 to 55 percent of bacillus subtilis, 5 to 10 percent of bacillus licheniformis, 5 to 10 percent of bacillus megaterium, 5 to 10 percent of candida utilis, 5 to 10 percent of rhizobium japonicum, 5 to 10 percent of bacillus mucilaginosus and 5 to 10 percent of lactobacillus plantarum, so that the microbial content of fermentation liquor reaches 6-10*10<9> per milliliter to obtain the complex microbial inoculum 707. Through long-term field verification, the complex microbial inoculum 707 has the advantages of reasonable formula and stable effect, can promote the growth of crops, improve fruit quality, promote early ripening, increase yield, improve the utilization rate of fertilizers and improve the ecological environment of soil, and has certain effect on disease prevention and disease resistance.

The invention discloses a greening substrate of quarry waste muck as well as a preparation method and application of the greening substrate. The method comprises the following steps of: selecting the quarry waste muck, artificially sieving with a sieve of 2cm (to remove impurities such as weed), and artificially crushing corn straws to 1-2cm; sealing at a high temperature, and decomposing; proportioning in parts by mass: 40 to 60 parts of muck, 30 to 40 parts of sewage sludge, 10 to 20 parts of straws, 50 to 100g / m<2> of sheep dung, 2 to 6g / m<2> of water holding agent and 15 to 45g / m<2> of bacillus licheniformis fertilizer; and mixing uniformly at normal temperature to obtain the greening substrate. The vegetation substrate or a soil improvement material and a formula can be produced on site, waste soil quantity is reduced, the cost is reduced, and land and vegetation resources are protected; and the greening substrate can be applied to integral greening, local soil dressing greening and bagged greening in semi-humid regions of north China and construction project active regions with seasonal drought, centralized and large rainfall.

The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g / T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W / W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g / T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.

The invention relates to a preparation method for a composite microbial seaweed organic liquid fertilizer, which comprises the following steps: (1) ingredient treatment: adding 0.1-0.6 part by weight of complex enzyme and 0.1-0.4 part by weight of microbial leaven to 100 parts by weight of seaweed-crushed liquor of which the water content is 70-90 percent, and evenly mixing; (2) aerobic fermentation: filling an ingredient obtained in the step (1) into a fermentation tank, and carrying out the aerobic fermentation for 5-8 days at the temperature of 40-60 DEG C under the condition that pH is 5-7 to obtain fermentation liquor; (3) filtering: filtering the fermentation liquor obtained in the step (2) to obtain filter liquor; and (4) compounding: adding functional fungicides to the filter liquor obtained in the step (3), wherein the functional fungicides comprise nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium bacteria; or / and adding bacillus subtilis and Bacillus licheniformis to the filter liquor obtained in the step (3); and carrying out blending on nitrogen phosphorus potassium (NPK) so as to enable the content of the NPK to reach the goal that N+P2O5+K2O isnot less than 4 percent.

The invention relates to a quick-acting multi-microorganism composite water flush fertilizer and a preparation method thereof. The quick-acting multi-microorganism composite water flush fertilizer is characterized by being prepared by mixing bacterial powder which is prepared by fermenting and culturing photosynthetic bacteria, pichiafermentanslodder, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, brevibacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, azotobocter chroococcum and actinomycetes. The density of the pichiafermentanslodder ACCC20319T in the quick-acting multi-microorganism composite water flush fertilizer is more than or equal to 2.0*10<8>cfu / g, and the densities of other bacteria are more than or equal to 2.0*10<9>cfu / g. The quick-acting multi-microorganism composite water flush fertilizer can improve the quality and yield of crops and shorten growth period of crops.

The invention discloses a method to prepare leaven of fertilizer and application, which is characterized by the following: allocating raw material with mass ratio at 10-80 wt industrial residue of ferment, 5-30 wt straw, 5-30 wt cake meal, 10-30 wt bran or rice bran, 1.5-2 wt aquacare, 0.5-1 wt NaCl and or amylaceum, and 1-30 wt brown ball Azotobacter (Azotobacter chroococcum), 1-30 wt bacillus megaterium (Bacillus megaterium), 1-30 wt bacillus licheniformis (Bacillus licheniformis), 1-30 wt plant lactobacillus (Lactobacillus plantarum) and 1-30 wt 1-5 strain of saccharomycopsis fibuligera (Saccharomycopsis fibuligera); producing the product.

The invention relates to a high-efficiency ferment for fermenting bean pulp, belonging to the biologic enzymolysis fermentation technical field. The high-efficiency ferment is formed by mixing bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus casei, saccharomyces cerevisiae and candida utilis. The fermentation technology comprises the following steps: crushing the bean pulp and corn; activating the strains; uniformly mixing the bean pulp and the fermentation liquor using a mixer; packing the mixed bean pulp in fermentation bag; fermenting the packaged bean pulp and processing fermented bean pulp: drying-crushing-detecting-packaging-finished product. The technology is simple and the product quality is stable and the content of the anti-nutrient factor in the bean pulp is eliminated and obviously reduced, therefore the bean pulp is a safe plant protein feeder for infant animal with balanced nutrient, good quality and low price and help for health growth of the infant animal, in replace of animal protein feeder of bone powder, fish powder or the like.

The present invention relates to a method for the production of a variant lipid acyltransferase comprising the steps of: (i) selecting a parent enzyme which is a lipid acyltranserase enzyme; (ii) modifying one or more amino acids to produce a variant lipid acyltransferase; (iii) testing the activity of the variant lipid acyltransferase on a galactolipid and / or phospholipid and / or triglyceride substrate; (iv) selecting a variant enzyme with enhanced activity towards galactolipids compared with the parent enzyme; (v) providing a Bacillus licheniformis cell; (vi) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding said variant lipid acyltransferase; and (iii) expressing said variant lipid acyltransferase in the cell under the control of a promoter sequence. The variant lipid acyltransferase can undergo post-translations modification, truncation and / or clipping, i.e., to remove a signal peptide. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g / l.

The invention provides a method for preparing an acid organic fertilizer by orientation fermentation of an organic waste. The method is characterized by comprising the following specific steps of: mixing bacillus subtilis, bacillus licheniformis, bacillus pumilus, saccharomyces cerevisiae, acetobacter aceti, trichoderma, aspergillus oryzae, nocardia and geotrichum candidum to obtain a first composite microbial bacteria agent; mixing the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, bacillus coagulans, lactobacillus acidophilus, lactobacillus plantarum, lactobacillus casei, pediococcus pentosaceus, the aspergillus oryzae, mucor racemosus and the nocardia to obtain a second composite microbial bacteria agent; adding the first composite microbial bacteria agentinto the organic waste and performing aerobic fermentation so as to form an intermediate fermentation product; and adding the second composite microbial bacteria agent into the intermediate fermentation product and performing anaerobic fermentation so as to form the organic fertilizer. The method has the advantages of capability of improving saline and alkaline lands, suitability for cultivation of acidophilous crops, effect of performing micro-ecological remediation on continuous cropping soil and low cost.

The invention relates to an environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture. An environment-friendly composite microbial water purifying agent comprises the following components in percentage by weight: 20 to 40 percent of bacillus subtilis, 10 to 20 percent of bacillus licheniformis, 10 to 15 percent of bacillus megaterium, 10 to 15 percent of brevibacillus laterosporus, 5 to 10 percent of bacillus pumilus, 5 to 20 percent of bacillus coagulans and 2 percent of biological activating agent. The high-concentration composite microbial preparation is prepared by screening eight strains of probiotics by simulating the physiological functions of normal microbial flora in an aquaculture environment and through scientific compatibility, so that division and cooperation amount the bacterial strains can be realized, a biological enhancement technology can be combined with an indigenous flora activating technology, complex problems in a culture water body can be solved completely, the using amount is small, the cost is low, and the competitive advantage is remarkable.

A bioorganic fertilizer is produced with cake dregs, farm animal excrement and stalk and straw as raw materials and through fermentation with the solid bacteria strain cultured by using lichen bacillus and subtilis bacillus, drying in air or stoving after being throughly decomposed, and crushing. During fermentation, the C / N ratio is controlled at 15-26, organic material water content is controlled at 38-52 % and microbe fermentation temperature is 50-70 deg.C. The present invention has wide material source, low cost and simple production process, and applying the fertilizer can raise the yield and quality of crops, improve physical and chemical characteristics of soil, reduce environmental pollution and shorten the growth period of crops.

The invention relates to a strain capable of degrading feather keratin efficiently and a screening method thereof. According to result of the analysis and identification of the sequence of 16SrDNA and the result of the identification of a Biolog system, F4 is determined to be Bacillus licheniformis and is named Bacillus licheniformis F4. The Bacillus licheniformis F4 was collected in China Center for Type Culture Collection with a collection number of CGMCC No.4229 on October 22th, 2010. The screening method comprises: primarily screening, separating, enriching, and secondarily screening to obtain the strain capable of degrading feather keratin efficiently. In the invention, the strain capable of degrading feather keratin efficiently is obtained by two steps of screenings under strict conditions, wherein primary screening conditions are a carbon source and a nitrogen source, and secondary screening conditions are complete degradation of feathers and measurement of enzymatic activity of keratin. The strain is inoculated into a fermentation culture medium containing complete feathers to perform fermentation culture for 60 hours, all barbules of the feathers are removed, the left feather stems and a major part of removed pinnules are degraded, and the feather removing and feather keratin degrading effects of the strain are obvious.

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.