128 results about "Immunocompetence" patented technology

The invention discloses bifidobacterium animalis and application thereof in allergic rhinitis products, and belongs to the technical field of microorganisms. The technical problem to be solved is that the existing common allergic rhinitis treatment means cannot meet the requirements of patients on safety, long-acting effect and treatment cycle. The invention provides an animal bifidobacterium subsp. Lactis Def +, and after an effective dose of animal bifidobacterium subsp. Lactis Def + is applied to a subject, the animal bifidobacterium subsp. Lactis Def + is found to be capable of remarkably relieving the allergic rhinitis symptom. Specifically, the symptoms of sneezing, dry nose and rhinocnesmus are reduced; the number of nasal septum mucosa intrinsic layer inflammation infiltration cells and nasal septum mucosa epithelium pathological changes are reduced; the IgA and IgE levels of serum are reduced; the levels of proinflammatory factors IL-4, IL-5 and IL-13 are reduced; the normal spleen index is maintained; the imbalance state of Th1 / Th2 and Th17 / Treg is effectively improved, and the body immunity is improved; a safe and long-acting scheme is provided for allergic rhinitis diseases.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention discloses an application of pleurotus eryngii purified polysaccharide in preparation of an intestinal care medicine. The pleurotus eryngii purified polysaccharide component has high immunocompetence, high-activity and high-purity polysaccharide is obtained after ethanol precipitation and organic solvent purification, and the high biological activity of the polysaccharide is reserved; the composition has the biological activity of increasing the production of short-chain fatty acid, reducing the inflammation level, improving the intestinal flora structure, influencing the body metabolism condition and protecting the intestinal barrier. Compared with other components in the pleurotus eryngii polysaccharide extraction process, the polysaccharide component obtained by the preparation method has the highest purity and the strongest biological activity, and can be used for preparing intestinal care medicines.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a biological product for overexpressing an OsARF7 gene to improvement of rice disease resistance, the nucleotide sequence of the OsARF7 gene is shown as SEQ ID NO.1, and the amino acid sequence of the OsARF7 gene is shown as SEQ ID NO.2. According to the invention, an OsARF7 overexpression rice strain is constructed by means of transgenosis. Tests show that the overexpression of the OsARF7 can significantly enhance the immunocompetence of the rice, for example, the expression level of a disease-resistant related gene is improved, active oxygen outbreak is significantly induced, and the resistance of the rice to false smut and bacterial leaf blight is significantly improved. The invention proves that the OsARF7 has a positive regulation effect on rice immunity, can be used for improving the disease resistance of rice, and has important significance for creating disease-resistant rice germplasm resources.

The invention relates to the technical field of bioactive substance preparation, and provides a lentil low-molecular-weight glucan with immunocompetence and a preparation method of the lentil low-molecular-weight glucan with immunocompetence, and the lentil low-molecular-weight glucan with immunocompetence is obtained by separating and purifying lentil. The extraction method comprises the following steps: crushing dried lentils, extracting by adopting a compound enzyme-assisted extraction method, freeze-drying to obtain crude polysaccharide, dissolving the crude polysaccharide with distilled water, performing deproteinization treatment by adopting a sevag method, separating the deproteinized solution through an anion exchange cellulose column, collecting effluent, and treating to obtain freeze-dried powder, and dissolving the freeze-dried powder, purifying, carrying out color development tracking, collecting a polysaccharide solution of a color development positive part, and freeze-drying to obtain the purified polysaccharide. The invention solves the problems in the prior art that deep exploration on the structure-function relationship and immunocompetence of the polysaccharide is lacked, and the development of the polysaccharide as an immunomodulatory functional raw material is limited.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The invention relates to the technical field of molecular markers, in particular to a molecular marker for early identification of immune traits of elephant male semi-fine wool sheep and application of the molecular marker. According to the present invention, the deterministic correlation between the genotype of the 102308292 site on the second chromosome and the immune globulin (IgA, IgG and IgM) level of the male semi-fine wool sheep is found for the first time; by detecting the genotype of the site, the future humoral immunity of the lamb can be predicted in the lamb stage, so that early seed selection is realized. The invention provides a powerful SNP marker for non-diagnostic purpose for disease-resistant breeding of male semi-fine wool sheep.

The invention provides a tuberculosis mRNA vaccine as well as a preparation method and application thereof. The invention firstly provides an antigen, which comprises the following antigen components: at least one fusion protein or chimeric protein formed by mycobacterium tuberculosis early secretion antigens Ag85A and Ag85B and / or immunocompetence fragments thereof, at least one mycobacterium tuberculosis PE / PPE family antigen Rv1759c (PE-PGRS family protein WAG22) or immunocompetence fragments thereof, and at least one immunocompetence fragment thereof, and at least one mycobacterium tuberculosis latent associated antigen Rv1813c or an immunocompetence fragment thereof; selectively, two or more of these antigens or immunocompetent fragments thereof may form a fusion protein and / or chimeric protein as an antigen component. The mRNA vaccine with multiple antigen components has a more effective effect on prevention of tuberculosis.

The present application relates to a kind of traditional Chinese medicine composition for treating chronic non-bacterial prostatitis and its preparation method and application.The traditional Chinese medicine composition includes bran warm Chinese yam, steamed leucomalus, alisma, cistanche, smilax glabra, sanqi head, fried radix codonopsis, fried white kidney bean and prepared rehmannia, with typical characteristics of homology of medicine and food, patient is easy to tolerate, can be taken long-term;And can improve the immune capacity of organism, kidney and yang, support and consolidate, completely relieve chronic prostatitis symptoms until radical cure.

The invention discloses bacillus subtilis Q16 and application thereof, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20252232. According to the invention, the bacillus subtilis Q16 is separated from the body of the large yellow croaker and is added into the feed for the large yellow croaker, so that the growth performance, digestion, oxidation resistance and immunity of the large yellow croaker can be remarkably improved, and the intestinal flora composition structure of the large yellow croaker and the gene expression related to immunity and metabolism are changed.

The invention relates to the technical field of biomedicine, in particular to an immunoregulation chiral material as well as a preparation method and application thereof, and the immunoregulation chiral material is formed by self-assembly of an immune active component and a delivery carrier. The immunocompetence component is selected from chiral peptide modified distearoyl phosphatidyl ethanolamine-polyethylene glycol, and the chiral peptide is formed by connecting 6-10 D-alanine monomers through amido bonds. The material can realize accurate targeting on target cells by virtue of specific three-dimensional recognition of chiral peptides and immune cell surface receptors, and reduces non-specific action on normal tissues; a delivery carrier represented by lipid nanoparticles can effectively protect chiral peptides from in-vivo enzymolysis and improve the cycling stability of the chiral peptides, and natural carriers such as exosomes can enhance the biocompatibility and tissue penetrability of the material, so that a new strategy is provided for immunotherapy of diseases such as tumors and the like; the compound is expected to be applied to clinical treatment as a novel immunotherapy drug.

The invention discloses flammulina velutipes polysaccharide nano-liposome as well as a preparation method and application thereof, and belongs to the technical field of biological functional materials. The preparation method specifically comprises the following steps: (1) sequentially performing hot water extraction, deproteinization, alcohol precipitation, dialysis and freeze-drying on flammulina velutipes raw material powder; (2) dissolving in a phosphoric acid buffer solution; (3) mixing and dissolving phospholipid, cationic lipid and a membrane regulator in an organic solvent; (4) mixing and ultrasonic treatment; and (5) carrying out reduced-pressure rotary evaporation, hydration with a phosphate buffer solution, ultrasonic treatment and extrusion. According to the flammulina velutipes polysaccharide nano-liposome and the preparation method thereof, cholesterol is replaced by phytosterol ester to prepare the flammulina velutipes polysaccharide nano-liposome, flammulina velutipes polysaccharide (FVP) is encapsulated in a water core in the liposome, the safety is higher, and the encapsulation efficiency, the storage stability and the immunocompetence of a system are improved. In-vitro experiments prove that the phytosterol ester lipidosome has the effect of promoting macrophage proliferation and nitric oxide generation, and the effect is obviously better than that of the traditional cholesterol lipidosome under the same condition.

The application discloses CpG-containing oligodeoxynucleotide (ODN) with the function of regulating the body's immune capacity and application thereof. The oligodeoxynucleotide in the application is different from the design concept of traditional A-type CpG ODN in sequence and structure, embodies the complex drug-making rule and overcomes the problem of traditional A-type CPG in drug-making. The oligodeoxynucleotide has excellent activity of regulating the body's immune function in vitro and in vivo, can stimulate the production of I, II and III type interferons, regulates the Th1 type immune response, and inhibits the Th2 type and Th17 type inflammatory response. Therefore, the oligodeoxynucleotide can prevent and treat allergic diseases caused by Th2 type immune response, including allergic rhinitis, atopic dermatitis, asthma, chronic obstructive pulmonary disease, eosinophil and Th17 related diseases, can prevent and treat the infection and spread of respiratory or non-respiratory pathogenic microorganisms including viruses, bacteria, fungi and parasites, can improve the immune level of the elderly and immunodeficient population, can convert "cold tumor" into "hot tumor", improve the effect of cancer immunotherapy, can prevent and treat central nervous system diseases related to immune dysfunction such as Alzheimer's disease, and can be applied to other diseases related to immune dysfunction.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

The invention relates to a mycelium extracted from ganoderma lucidum and a preparation method of a culture medium of the mycelium, belongs to the technical field of mycelium extraction, and aims to solve the problems that in the prior art, ganoderma lucidum mycelia grow slowly and are prone to pollution, the content of effective components is unstable, and molecular identification is difficult. The invention provides the solid culture medium prepared from the saussurea involucrata purple yellow extract, the extract is formed by combining effective components of saussurea involucrata and sodium alginate, the growth rate and metabolism efficiency of ganoderma lucidum hyphae can be improved, active substances such as polysaccharides and triterpenes are effectively enriched, and the yield of the ganoderma lucidum hyphae is increased. Meanwhile, the stress resistance and the survival rate of the mycelium under the stress conditions of oxidative stress, pathogen invasion and the like are enhanced, the pollution risk in the culture process is reduced, the biosynthesis efficiency and the extracellular secretion level of key secondary metabolites are improved, the immunocompetence and the bioavailability of the product are further improved, and the production cost is reduced. And a stable and reliable technical support is provided for large-scale and high-quality production of ganoderma lucidum.

The invention discloses Pseudomonas mendogensis Z3-4 capable of inducing tobacco bacterial wilt immunity and application of the Pseudomonas mendogensis Z3-4. The Pseudomonas mendogensis Z3-4 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, the preservation center number is CGMCC No.35660, the preservation date is August 18, 2025, and the Pseudomonas mendogensis Z3-4 is classified and named as Pseudomonas mendogensis. The strain Z3-4 is an efficient tobacco root colonization bacterium, and can significantly induce the immunocompetence of tobacco to bacterial wilt. The strain Z3-4 is used as a tobacco bacterial wilt resistant vaccine, is applied by adopting a root irrigation method, can effectively prevent and treat tobacco bacterial wilt, and has a wide application prospect.

The invention belongs to the technical field of gene engineering, and particularly relates to recombinant lactobacillus for expressing clostridium perfringens multiple antigens of targeted dendritic cells as well as a construction method and application of the recombinant lactobacillus. The recombinant lactobacillus contains an expression vector for expressing a fusion gene; the fusion gene comprises sequences for coding and expressing a PgsA '-fimA fusion protein, a LysM-Fba fusion protein, an LP1261-NetB fusion protein, a phi54 targeting element and a Pas escape element; a sequence for coding the PgsA '-fimA fusion protein is shown as SEQ ID NO: 1, a sequence for coding the LysM-Fba fusion protein is shown as SEQ ID NO: 2, and a sequence for coding the LP1261-NetB fusion protein is shown as SEQ ID NO: 3. When the recombinant lactobacillus is used for immunizing animals, mucosal immunity, cellular immunity and humoral immunity reactions can be induced, the immunocompetence of organisms can be comprehensively enhanced, and the recombinant lactobacillus can be used for preparing oral vaccines for prevention and control of chicken necrotic enteritis.

Disclosed are methods for determining the immunological status of the adaptive immune system of a subject by identifying and quantifying rearranged DNA (and / or subsequently transcribed RNA) sequences encoding T cell receptor (TCR) and / or immunoglobulin (IG) polypeptides, in a lymphoid DNA-containing sample from the subject. TCR and / or IG sequence diversity and sequence distribution permit immunocompetence and immune repertoire assessment and reflect the degree of T cell or B cell clonality and clonal expansion in the sample. Methods for stratifying patient populations on the basis of immunocompetence including likelihood of responding to immunotherapy are also described.

The application provides a lactic acid bacteria expressing an African swine fever virus fusion antigen and an application thereof. The lactic acid bacteria are integrated with a fusion gene, the fusion gene comprising a gene encoding an OprI protein and at least one gene selected from genes encoding a PP62 protein, an MGF505-3R protein, an EP364R protein, a K145R protein and a DP96R protein, and a starting strain of the lactic acid bacteria is Lactobacillus plantarum. Using the lactic acid bacteria to immunize animals can induce mucosal immunity, cellular immunity and humoral immunity responses, and comprehensively enhance the immune capacity of the body, and the lactic acid bacteria can be used for preparing an oral vaccine for the prevention and control of African swine fever.

The application provides an antigen nanoparticle and a preparation method and vaccine preparation application thereof, and relates to the technical field of biotechnology.The antigen nanoparticle provided by the application comprises an antigen and poly( maleic anhydride-ALT-1-octadecene) grafted with polyethylene glycol, the antigen nanoparticle has good stability and high immunocompetence, and can be used for the preparation of a vaccine.The preparation method of the antigen nanoparticle provided by the application is convenient to operate, parameters are controllable, reproducibility is good, and the reaction is carried out in a water environment, the condition is mild, and the influence on the structure of the antigen is small.The vaccine provided by the application comprises the antigen nanoparticle and an adjuvant, and the inventor has found that the antigen nanoparticle and the adjuvant have a synergistic effect, and the combination of the two can further improve the immunization effect of the vaccine.

The invention relates to the technical field of livestock and poultry breeding, and particularly discloses combined application of prebiotics and probiotics, the prebiotics are propyl inulin nano prebiotics, and the probiotics are plant lactobacillus. 0.1 g / kg of propyl inulin nano prebiotics and 109 CFU / kg of plant lactobacillus are added into the broiler feed, so that the weight gain ratio of the broiler feed is reduced to 1.67, the pectoral muscle rate is increased to 30.4%, the anti-oxidation defense and immune regulation capabilities are improved, the intestinal microbial metabolic pathway is remarkably changed, and the growth of the broiler feed is promoted. The combined application of prebiotics and probiotics realizes triple improvement of broiler chicken production performance, immunocompetence and intestinal health, and provides a new way for promoting green and safe development of broiler chicken breeding industry.

The invention discloses a novel vegetable health magnesium resistance inducer as well as a preparation method and application thereof, the main components of the vegetable health magnesium resistance inducer are trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs), and the components have the characteristics of no toxicity, harmlessness, no pollution and the like. The invention finds that the'vegetable health magnesium 'resistance inducer can activate plant hormone signals, enhance plant immunity and resist virus diseases. The composition can be sprayed to vegetable crops before the high-incidence period of vegetable virus diseases to prevent and treat virus diseases, is simple and convenient, has strong operability, and can effectively improve the broad-spectrum resistance of plants to various vegetable virus diseases. Compared with traditional pesticides, the composition is more green and safer, meets the requirements of sustainable and healthy development of agriculture, and has huge application potential.

The application discloses a tomato Soly04g076730 Gene or its encoded protein is applied to resisting plant geminivirus. Soly04g076730 The gene is transiently expressed or stably genetically transformed in a plant leaf. When a virus invades the plant, the plant immune response is rapidly induced, and the disease resistance of the plant is improved. The Soly04g076730 protein is a secretory protein. The plant immune ability can be improved by expressing or externally applying the protein in the plant, and the virus transmission and replication can be effectively inhibited.

The invention discloses a composite milk adjuvant, a preparation method and application of the composite milk adjuvant in vaccines, and belongs to the technical field of medicines. The technical problems to be solved are relatively weak cell immunocompetence, relatively poor immunogenicity and relatively weak immunoregulation capability. According to the technical scheme, squalene, polyoxyethylated castor oil 35 and poloxamer 407 are used as raw materials to prepare a basic formula of the milk adjuvant, an immunologic stimulant is added on the basis to prepare the composite milk adjuvant, and the physicochemical properties and safety of the composite milk adjuvant are represented. The synergistic effect of the milk adjuvant on humoral immunity and cellular immunity is further evaluated by being matched with a recombinant tuberculosis antigen Ag85B to immunize mice, and data support is provided for application of the milk adjuvant in development of novel vaccines such as tuberculosis vaccines and the like. The composite milk adjuvant prepared by the invention not only keeps good humoral immunity and induces generation of high-level IgG1 and IgG2a, but also remarkably improves the response level of cellular immunity.

This invention belongs to the field of microbial technology, specifically relating to a *Clostridium butyricum* strain, its culture conditions, and applications. This invention provides a novel *Clostridium butyricum* strain with strong resistance to high temperatures, acids, alkalis, and salts, exhibiting high biocompatibility and no significant toxic side effects or hemolytic activity. Therefore, the *Clostridium butyricum* H2-2 is a potential probiotic strain that can be used as feed or feed additive in pig, poultry, and aquatic feeds for animal husbandry, strengthening the intestinal barrier and enhancing animal immunity; it also holds promise for use as an additive in human food, pharmaceuticals, and health products.

The invention relates to the technical field of biological medicines, and particularly discloses an egg yolk antibody for resisting canine interleukin as well as a preparation method and application of the egg yolk antibody. The egg yolk antibody is prepared by taking immunocompetence antigen epitope peptide of canine interleukin-31 as an immunogen, the antigen epitope peptide is obtained by predicting key antigen epitopes of IL-31 through an Antigenic tool and performing series optimization, a coding gene of the antigen epitope peptide is optimized by an escherichia coli codon to construct a recombinant expression vector, and high-purity antigen is obtained by inducible expression and purification. A laying hen is immunized by the antigen, after basic immunization and two times of booster immunization, the egg yolk antibody is extracted from egg yolk and purified to obtain the egg yolk antibody, and the ELISA of the egg yolk antibody is obviously superior to that of an antibody prepared by commercial canine IL-31 protein immunization. The egg yolk antibody can be specifically combined with canine IL-31, the histamine level in serum of a canine with pruritus is remarkably reduced, the atopic dermatitis related pruritus symptoms are effectively relieved, an efficient and safe biological treatment preparation is provided for canine IL-31 mediated pruritus diseases, and the egg yolk antibody has wide clinical application prospects.

The invention belongs to the technical field of livestock breeding, and discloses a preparation method and application of a bacterium-enzyme synergistic fermentation material for improving the reproductive performance of first-born sows and the growth performance of piglets. The invention discloses a fermentation material which is prepared from the following raw materials: dried alfalfa, palm meal, sugar, compound enzyme and compound bacteria, the compound enzyme is composed of cellulase, mannase and xylanase, the invention provides a fermented material, the fermented material is added to a basic feed for feeding first-fetus lactating sows, and the result shows that the daily average feed intake of the lactating sows can be remarkably improved, the backfat loss of the lactating sows is reduced, and the reproductive performance and milk components of the sows are improved; the antioxidant capacity and the immunocompetence of the piglets can be improved, and the inflammation level of piglet bodies can be reduced.

The invention provides a postbiotic immunomodulatory preparation based on bifidobacterium longum CD11 and a preparation method of the postbiotic immunomodulatory preparation. Bifidobacterium longum CD11 is used to prepare a metagen. The immunocompetence of RAW264.7 macrophages can be promoted by thermal inactivation of bifidobacterium longum CD11 metabolites, cell walls, cell membranes and cell content metabolite components, and the cell wall component dose-dependently promotes the proliferation of the RAW264.7 macrophages and inhibits the relative expression of mRNA genes of inflammatory factors TNF-alpha and IL-1beta. In addition, a peptidoglycan (PGN) metagen from a cell wall component can remarkably inhibit relative expression of TNF-alpha and IL-1beta mRNA genes of RAW264.7 macrophages, so that inflammation is recovered to a health level (NC group level). Therefore, the bifidobacterium longum CD11 can be used as a potential immunomodulatory metabiotic strain.

The invention discloses a tuna red meat immunity enhancing peptide and application thereof in enhancing immunity, and belongs to the technical field of biology. According to the invention, polypeptides with immunocompetence are extracted from tuna red meat, and the polypeptides comprise FLQGF and LAKFPDF. Experiments prove that the polypeptide prepared by the invention has no obvious toxic effect on cells, and can improve the proliferation rate and the phagocytic rate of macrophages and the release rate of NO to different degrees. An in-vitro immunomodulatory activity result shows that the LAKFPDF and the FLQGF can activate a TLR4 dependent NF-kB / MAPK signal channel, regulate and control the immune response of macrophages and play an immunomodulatory role. Research results of the invention provide a basis for high-value development and utilization of tuna red meat, and provide a new resource for preparation of immunopotentiation products.

The invention provides a plant polysaccharide with an immunomodulatory function, an extraction method thereof and an immunomodulator, and relates to the technical field of plant polysaccharides. The extraction method provided by the invention comprises the following steps: carrying out alcohol extraction on plant raw materials, and then separating alcohol extraction residues; carrying out water extraction on the alcohol extraction residues, and separating to obtain a water extract; carrying out concentration and alcohol precipitation on the water extract, and centrifuging to obtain a polysaccharide mixture; deproteinizing, dialyzing and freeze-drying the polysaccharide mixture to obtain plant polysaccharide; the plant raw material is one of clover leaves, fairy leaves and banana flowers. According to the extraction method provided by the invention, the plant polysaccharide with relatively high immunocompetence can be obtained, and the plant polysaccharide effectively activates immune effector cells such as macrophages and promotes secretion of cytokines through a bidirectional immunoregulation effect, so that safe raw materials can be provided for developing products for enhancing body defense and maintaining immune balance.