6 results about "Immunomodulatory peptide" patented technology

The application relates to the field of biotechnology, and particularly discloses an immunoregulatory peptide as well as a preparation method and application thereof. Three new polypeptides with ideal immunoregulatory effects are found through specific enzymolysis and screening, and the polypeptides have the amino acid sequences shown in any one or more of SEQ ID No. 1-3. The application enriches the types of milk-derived bioactive peptides, and provides new raw materials for preparing immunoregulatory effect products.

This invention specifically relates to spirulina immunomodulatory peptides, their preparation methods, and applications, belonging to the field of protein bioactive peptide technology. This invention is the first to screen immunomodulatory peptides Ser-Pro-Ser-Trp-Tyr (SPSWY), Met-Phe-Asp-Ala-Phe (MFDAF), and Phe-Gly-Arg-Phe-Arg (FGRFR) from spirulina phycocyanin. The structure and immunomodulatory activity of peptides SPSWY, MFDAF, and FGRFR were clarified. Peptides SPSWY, MFDAF, and FGRFR can enhance macrophage cell viability and phagocytic capacity, and also increase the secretion of NO and cytokines by macrophages. Their immunomodulatory activity also exhibits good stability. Furthermore, peptides SPSWY, MFDAF, and FGRFR have the advantages of being safe, non-toxic, and having high biological activity.

Aspects of the present disclosure include methods for modulating macrophage activity. Methods according to certain embodiments include contacting a macrophage with a mannose receptor (CD206) binding agent in a manner sufficient to modulate activity of the macrophage. Methods for converting a phenotype of a macrophage from an M2 phenotype to an M1 phenotype are also provided. Methods for inhibiting growth of a CD206-expressing cell as well as methods for treating a subject for a neoplastic condition (e.g., cancer) or a condition associated with chronic inflammation are described. Immuno-modulating peptides suitable for use in the subject methods are also presented.

The application discloses a preparation method of lactoferrin immunomodulatory peptide and application thereof. The application provides recombinant escherichia coli which is escherichia coli capable of expressing fusion protein SUMO-LF(IMP); the fusion protein SUMO-LF(IMP) is fused by a SUMO tag and lactoferrin immunomodulatory peptide (SEQ ID NO:1 or SEQ ID NO:2 or SEQ ID NO:3). The recombinant strain of the application can efficiently express target protein, the expressed immunomodulatory active fragment retains natural immunomodulatory activity, and the prokaryotic expression system has the advantages of low culture cost, short fermentation period, easy separation and purification of protein and the like, the constructed strain can produce immunomodulatory active fragments on a large scale, provides a core production strain for product development in the fields of immunomodulators, biological pharmaceuticals, functional food additives and the like, and has important industrial application value and market prospect.