383 results about "Phycocyanin" patented technology

The invention discloses a technique for extracting phycocyanin, chlorophyll and spirulina polysaccharide in spirulina, comprising the following steps: taking and weighing fresh spirulina, centrifuging and washing the spirulina, weighing spirulina clay, adding water into the supernatant, stirring evenly, and freezing and thawing twice to break the cell wall, after the second dissolving, adding water, placing for 2-8 hours, centrifuging to obtain supernatant and sediment A, extracting the sediment A by a Soxhlet extracted method with ethanol to divide the sediment A into extraction and sediment B, wherein the supernatant is used for extracting phycocyanin, the sediment A is used for extracting sodium zinc chlorophyllin and the sediment B is used for extracting spirulina polysaccharide. The extraction technique extracts the active substances such as phycocyanin, chlorophyll and spirulina polysaccharide, and fully and effectively utilizes the prior spirulina resource and avoids the resource waste.

The invention discloses a method for separating high purity phycocyanin from spirulina, comprising the following steps: adopting 0.05-0.5M of phosphate buffer to elute a spirulina powder raw material by an elution method, enabling the phycocyanin to be effused from spirulina cells, adding soluble chitosan to phycocyanin crude extract, controlling the mass concentration of chitosan in the phycocyanin solution to be 0.1-1%, and collecting supernate by centrifuging; adding activated carbon to the supernate, centrifuging and collecting supernate, adopting ammonium sulfate precipitation with the mass concentration of 30-60% to precipitate the phycocyanin in the solution, adopting ion-exchange chromatography, and performing gradient elution by phosphate buffer containing sodium chloride to obtain the high purity phycocyanin solution. The invention has the advantages of low cost, short elapsed time, convenient and rapid operation, low energy consumption, and easy massive preparation, is an efficient method for massively preparing phycocyanin with high purity and high activity and is favor of scaled development and utilization of the phycocyanin in our country.

The present invention is related to a multiple emulsion of the water-in-oil-in-water (W/O/W) type for stabilization of natural coloring agents, such as anthocyanin, phycocyanin, carthamus, huito, calcium carbonate, carmine and carminic acid, against changes in pH values, oxidation and light as well as against ingredient interactions.

The invention belongs to the technical field of medicament, and particularly relates to preparation and application of phycocyanin extract. The phycocyanin extract is prepared by the following steps of: crushing spirulina cell walls of spirulina powder to obtain a crude extract by a repeated freeze-thawing method at the temperature of between 20 DEG C below zero and 4 DEG C; depositing the crude extract to obtain a crude protein extract by using 50 to 60 mass percent of ammonium sulfate; and then extracting and purifying the crude protein extract by adopting double aqueous phases, and removing salt by using excess infiltration to obtain phycocyanin extract solution. The phycocyanin extract mainly comprises phycocyanin, and the aqueous solution of the phycocyanin extract has the absorbance characteristics that A620/280 is slightly equal to 3.0+/-0.2 and A620/650 is slightly equal to 2.2+/-0.1. The phycocyanin extract is obtained by mainly combining the processes of ammonium sulfate deposition, double aqueous phase extraction, dialysis and salt removal and the like; and the phycocyanin extract administrated to S180 sarcoma mice with different dosages shows good tumor inhibiting effect and has no obvious toxicity on the spleen and the thymus gland.

The invention discloses a lake water body blue-green algae abundance estimation method based on remote sensing. The method comprises the following steps: carrying out high-spectrum remote sensing measurement on a lake water body by utilizing the concentration ratio of phycocyanin and chlorophyll to represent the blue-green algae abundance, selecting the ratio of remote sensing reflective rates of the two specific wave lengths and a function thereof as characteristics of blue-green algae, establishing an estimation model of the lake water body blue-green algae abundance, and estimating the lake water body blue-green algae abundance based on remote sensing. The method provided by the invention adopts a surface feature spectrograph or an onboard high-spectrum imager to carry out high-spectrum remote sensing on the lake water body, and the remote sensing reflective rate required for estimation can be obtained through performing radiation correction and atmospheric correction on aviation satellite high-spectrum data. The method provided by the invention has the advantages that the accuracy is high, the model is simple, the remote sensing reflective rates of the two wave lengths are selected, not only can the estimation on the water body blue-green algae abundance measured by the surface feature spectrograph be realized, but also the estimation on the blue-green algae abundance by virtue of aviation high-spectrum remote-sensing images is realized.

The invention relates to a water quality detection sensor. In the sensor, a first irradiation light source is arranged on the left of a first collimating lens, a dichroic beam splitter is arranged above a second collimating lens, a second irradiation light source is arranged below the second collimating lens, and the first irradiation light source and the second irradiation light source are connected with a control module respectively; a sample pond is arranged between a first photoelectric detector and the dichroic beam splitter; a focusing lens is arranged in the direction which is perpendicular to irradiation light; an optical filter is arranged below the focusing lens, and is positioned at a light inlet of a second photoelectric detector; signal output ends of the first photoelectric detector and the second photoelectric detector are connected with a signal acquisition and processing module; and the signal acquisition and processing module is used for calculating the content of chormophoric dissolved organic matters and the content of phycocyanin of a water sample. In the water quality detection sensor, the concentration of the chormophoric dissolved organic matters in water is detected while the concentration of cyanophytes is detected to detect the eutrophication of lake and perform early warning on the breakout of the cyanophytes.

The invention provides a preparation method of carbon nanotube-chitosan-phycocyanin nanoparticles, which comprises the following steps: modifying carboxylated carbon nanotubes with water-soluble chitosan in a non-covalent mode to obtain a water-soluble multiwall carbon nanotube-chitosan (MWCNT-CS) compound; and coupling the MWCNT-CS compound with high-purity spirulina phycocyanin to obtain the biomedical material carbon nanotube-chitosan-phycocyanin nanoparticles (MWCNT-CS-PC). The method has the advantage of simple preparation technique; and the obtained biomedical material has the advantages of favorable water solubility and biocompatibility, has favorable absorption characteristic in both visible light and near-infrared light regions, and is expected to be applied to photodynamic therapy and photo-thermal therapy on cancer cells.

A means and method for treating inflammation and pain in animals is described. The compounds include phycocyanin in a pharmaceutically acceptable carrier. Phycocyanin has been found to be a COX-2 selective agent, and therefore alleviates pain and inflammation without the side effects that occur with NSAIDs that are not COX-2 selective.

The invention provides a method for eutectic solvent and salt double aqueous phase extraction of phycocyanin from spirulina. The method comprises steps of supersonic-wave-assisted eutectic solvent extraction, eutectic solvent and salt double aqueous phase extraction, secondary double aqueous phase extraction and recycle of the eutectic solvent and phycocyanin. According to the method, the recyclable eutectic solvent is taken as an extraction solvent and forms a double aqueous phase system together with salt for separation and purification. The advantages of the eutectic solvent and double aqueous phase are combined, so that the method is simple to operate and saved in time, the yield and purity of phycocyanin are obviously improved, and the method is suitable for massive industrial production.

The invention discloses a method for preparing phycocyanin by using bloom cyanophyte. Said invention uses the fresh bloom cyanophyte as raw material, and adopts the following steps: first step, extracting crude filtrate; dehydrating fresh bloom cyanophyte, adding buffer solutio, mixing them uniformly, frezing, takingout and thawing at room temperature, freezing and thawing for 3-5 times and filtering by using plate and frame; second step, ultrafiltering and purification; using hollow fibre ultrafiltration device whose trapping molecular weight is 1000-200000 daltons to remove various substances with smaller molecular weight from crude extract passed through microfiltration by using ultrafiltering membrane; third step, settling-clearing purification; removing most of small molecules from phycocyanin portion purified by means of ultrafiltration, at the same time making concentration and storing the concentrated phycocyanin portion at a certain temperature; and fourth step; hydroxyapatite column chromatography to make purification.

The invention discloses a preparation method of food grade phycocyanin protein, comprising the following steps: breaking the wall of the spirulina power; adding weak acid salt buffer solution in the wall broken spirulina power for digestion; carrying out solid-liquid separation on the spirulina power leach liquor; extracting supernatant; filtering the supernatant to obtain the crude extract; and purifying the crude extract to obtain the concentrated solution; and spraying and drying the concentrated solution. The invention also discloses a food grade phycocyanin protein prepared by the method, wherein the purity (OD620/OD280) is larger than 1.0; the content of the phycocyanin protein is larger than 50%; the content of allophycocyanin is larger than 10%; the content of phycoerythrin is larger than 2%; and the content of other proteins is smaller than 2%. The method has low production cost, is simple and effective, and is suitable for preparing mass food grade phycocyanin proteins.

The invention discloses a preparation method of high-purity phycocyanin, and is characterized in that the preparation method comprises the following steps: (1) taking a fresh spirulina powder, fully mixing with a phosphate buffer solution evenly, repeatedly freezing and thawing for 7-10 times to break and remove cell walls, centrifuging to remove spirulina mud, and thus obtaining a supernatant; (2) adopting a two-step precipitation method with a 20%-30% ammonium sulfate and a 40%-60% ammonium sulfate to obtain a phycocyanin crude extract; (3) after dialyzing the crude extract, loading the sample onto a weak anion exchange column DEAE Sepharose FF, carrying out gradient elution after ion exchange, collecting an outflow component with A620/A280 of more than 3; and (4) dialyzing the collected sample, then loading the sample onto a strong anion exchange column SOURCE30Q, carrying out gradient elution after ion exchange, collecting an outflow component with A620/A280 of more than 4, again carrying out one-time ammonium sulfate precipitation concentration, and thus obtaining the high-purity phycocyanin having the purity of more than 4.5. The extraction purification method is simplified in process, wide in source of the raw material spirulina, simple in required equipment, and high in purity of the product, and has quite high application value.

A particle detection system with a detection mechanism that includes detectors positioned to detect two different ranges of fluorescence produced by particles in the fluid in a flow chamber. Each of the detectors is arranged to generating a trigger signal whenever fluorescence is detected. The system and related method enhance the accuracy and sensitivity of blue-green algae monitoring by utilizing imaging flow cytometry combined with particle analysis and the measurement of the ratio of each particle's phycocyanin to chlorophyll b detected by using the two detectors configured for detection of two different fluorescence ranges, one associated with the phycocyanin and the other associated with the chlorophyll b. Optionally, captured images may be used in comparison to known images of a library of images.

The invention discloses a method and a culture device for coupling biogas fermentation with microalgae culture. The method comprises the following steps: carrying out biogas fermentation to obtain biogas and biogas slurry; filtering the biogas slurry to remove solid granules, diluting the biogas slurry to serve as a microalgae culture medium, filling the diluted biogas slurry in a microalgae culture reactor to serve as the microalgae culture medium, filling the bottom of the microalgae culture solution in the microalgae culture reactor with biogas to serve as a carbon source, culturing under a light condition, and drying and removing water vapor from the biogas flowing out from the microalgae culture reactor for use; when harvesting the microalgae, dewatering to obtain microalgae sludge and culture solution, extracting biolipid, phycocyanin and other products with high added values from the microalgae sludge, and carrying out biogas fermentation on the residual microalgae residues and the culture solution in a biogas digester as biogas fermentation materials. The entire process of the method disclosed by the invention is driven by the solar energy, and carbon, nitrogen, phosphorus and other microelements are recycled, thereby satisfying the requirements of green energy source and sustainable development.

The present invention includes the following steps: hypotonic process to prepare extraction of algae, mixing hydroxyl apatite and the extraction of algae and eliminating the other components of extraction of algae in the supernatant; washing hydroxyl apatite with combined biliprotein with low concentration phosphate buffering liquid to eliminate other components; eluting phycocyanin or phycoerythrin from hydroxyl apatite with medium concentration phosphate buffering liquid and collecting phycocyanin or phycoerythrin; and eluting hydroxyl apatite with higher concentration phosphate buffering liquid to obtain allophycocyanin component. The present invention has high yield and purify of biliprotein and is favorable to production in industrial scale.

A method for using heavy metal mild pollution spirulina to extract phycocyanin, and using its algal residue as principal substrate to adsorb and remove lead, arsenic and cadmium in the water, pertains to the field of environmental biotechnology. The method can be widely used in innocent treatment of mild heavy metal pollution spirulina without accessing market standards in industrial production. The inventive method has main features the method adopts the features of mild heavy metal pollution that it only stick on cell walls, uses a improved purification method to obtain spirulina further processed product phycocyanin that conforms to safety criterion and has higher economic value, wherein, the accompanying diagram is a mass spectrogram of the extracted phycocyanin; and fixs the algal residues, for preparing biological adsorbent for obtaining lead, arsenic, cadmium and other heavy metals.

The invention provides a method for extracting phycobiliprotein from spirulina. The method comprises the following steps: adding water into spirulina mud to obtain a spirulina cell suspension, performing ultrasonic treatment to obtain spirulina lysate, centrifuging the lysate and taking the lysate, sequentially filtering through filter membranes which are respectively 0.8mu m and 0.45mu m, to obtain phycocyanin crude extracting liquid, then sequentially ultrafiltrating the crude extracting liquid through an ultrafiltration membranes with molecular weight cut-off of 300KD and 100KD, collecting a filtrate of the molecular section, and performing freezing and drying to the filtrate to obtain phycobiliprotein powder. According to the method, the defect of using chemical reagent to extract phycobiliprotein in the prior art is overcome, no residuals exist in the preparation, and the environment pollution does not exist. The extraction rate of the phycobiliprotein is greater than 80%, the content of phycobiliprotein is more than 60%, and the purity of the phycobiliprotein is more than 1.5. The overall technological process is safe and reliable, fast and efficient. Compared with the traditional method, by using the method, the content of the phycobiliprotein is high before using of chromatography, the phycobiliprotein with the purity not only can meet the requirement of food and cosmetic industries, but also can be applied to aspects of medical and health products, and the like.

The invention refers to a making method of algae blue protein by using seawater spirulina as raw material. The current making method has long technical course, long cycle of extraction, high cost, low efficiency, low application value and mostly is made at low temperature. The invention can largely and at low cost extract higher-purity algae glue protein, is applied to research and development of natural pigment, health food and new sea medicament.

The invention provides a method for simultaneously separating and purifying reagent-grade phycocyanin and allophycocyanin. The method comprises the following steps: breaking walls of blue-green algae and carrying out solid-liquid separation to obtain a phycobiliprotein crude extraction solution; carrying out two-step salting-out to obtain sediment; dissolving the sediment by utilizing a phosphate buffering solution; by taking the phosphate buffering solution as a dialysis solution, dialyzing a dissolved solution so as to obtain a dialyzed phycobiliprotein solution; finally, carrying out cellulose column chromatography and hydroxylapatite column chromatography to obtain the reagent-grade phycocyanin and the reagent-grade allophycocyanin. According to the method provided by the invention, water-bloom fresh blue-green algae is used as a raw material and the reagent-grade phycocyanin and the reagent-grade allophycocyanin are separated and purified by adopting salting-out combined with column chromatography; compared with a traditional chromatography process, high-purity phycocyanin and allophycocyanin can be simultaneously extracted and purified under the premise of considering the recovery rate of protein, and the efficiency of comprehensively extracting and purifying high-value protein in blue-green alga cells is improved and can be amplified.

The invention discloses a method of extracting phycocyanin from an ionic liquid aqueous two-phase system and particularly relates to a method of extracting phycocyanin from spiral seaweeds. The method comprises the following steps of: mechanically crushing spiral seaweed materials, adding into proper amount of dipotassium phosphate aqueous liquor and soaking, placing into an ultrasonic crusher to carry out wall-breaking treatment on spiral seaweed cells; extracting crude extracting liquor of spiral seaweed phycocyanin by utilizing the ionic liquid aqueous two-phase system formed by ionic liquid, dipotassium phosphate and water; and adopting column chromatography for separating and purifying the ionic liquid containing the phycocyanin to obtain the phycocyanin fine powder.

The invention discloses an anti-aging cosmetic and a preparation method thereof. The anti-aging cosmetic comprises the following components which are, by mass percentage, 10-20% of spirulina phycocyanin, 1-2% of haematococcus pluvialis extract, 2-5% of vitamin E, 5-8% of prinsepia utilis royle oil, 25-35% of oil phase raw material and 30-55% of aqueous phase raw material; and the sum of the mass percentages of all the components is 100%. The oil phase raw material is melted and fully evenly stirred, the aqueous phase raw material is heated and sterilized with stirring, the oil phase raw material and the aqueous phase raw material are evenly dispersed to be emulsified, the spirulina phycocyanin, the haematococcus pluvialis extract, the vitamin E and the prinsepia utilis royle oil are added, natural cooling is performed after uniform stirring, and the anti-aging cosmetic is obtained. The anti-aging cosmetic is high in permeability and can improve cutin degeneration and have effects of moisture preservation and anti-cracking. Besides, the anti-aging cosmetic is good in safety, non-toxic and free of side effects to the human body and has no stimulation effect on the skin of the human body.