84 results about "Extraction Purification" patented technology

The invention relates to the technical field of polysaccharide extraction, in particular to a dendrobium huoshanense protocorm polysaccharide extraction and purification method which comprises the following steps: cleaning dendrobium huoshanense protocorm, freeze-drying, crushing and sieving to obtain raw material powder; measuring initial raw material characteristic parameters of the raw material powder, and setting initial extraction parameters according to the initial raw material characteristic parameters; mixing the raw material powder with an extraction solvent, monitoring the concentration of polysaccharide in an extracting solution in real time in the extraction process, and calculating an extraction activity index; comparing the extraction activity index with a preset extraction activity threshold value, and adaptively adjusting extraction parameters according to a comparison result; the extracted extracting solution is purified, and the impurity characteristics of the extracting solution are monitored in real time in the purification process; the purification parameters are adaptively adjusted based on the characteristics of the impurities in the extracting solution obtained through real-time monitoring. The method can improve the polysaccharide yield stability and improve the product quality and consistency.

The invention provides an extraction and purification method of tetraacetyl phytosphingosine. Comprising the following steps: pretreatment and plate frame filtration of tetraacetyl phytosphingosine fermentation liquor, flash evaporation drying of a thallus wet filter cake, microwaves of bacterial powder and reflux extraction of a solvent, reverse extraction, enrichment and impurity removal of an extracting solution, preparation of a crude extract, primary crystallization of the crude extract by a polar solvent, alternate solvent crystallization of a crystallized primary product and the like. The tetra-acetyl phytosphingosine is extracted from the bacterial powder obtained by flash evaporation and drying after thallus separation by adopting a microwave and extraction solvent condensation reflux technology, and the method has the advantages of small solvent dosage, high extraction speed and high extraction yield. Besides, silica gel column chromatography separation and purification are replaced by means of reverse extraction, enrichment and impurity removal and alternate solvent crystallization, use of a mixed solvent and generation of silica gel solid waste are avoided, the purposes of saving cost and reducing environmental pollution can be achieved at the same time, and the method has wider application prospects and better industrial production potential.

The utility model relates to plant extraction technical field discloses a kind of plant exosome extraction and purification machine, including bottom plate, the left side fixedly connected with bracket of bottom plate, the output end fixedly connected with multiple blades of motor one, the outer wall of wall breaking box is connected with filter box, the inner wall fixedly connected with multiple activated carbon plate of filter box, the bottom of filter box is connected with burette, the outer wall fixedly connected with piston of burette, the top fixedly connected with fixed mechanism of bottom plate, the fixed mechanism is used for fast installation.In the utility model, open motor one, blade breaks the wall of plant, activated carbon plate filters out residue and pigment, piston adjusts the flow rate and dosage of juice, let juice drop into mixed test tube, simplify the operation of early separation filtering of plant solvent, reduce the time cost of experiment, improve the efficiency of extraction and purification, increase practicality, expand the scope of application.

The invention belongs to the technical field of plant active component extraction and purification, and particularly provides a plant extract purification method based on a deep-eutectic solvent. A plant extract purification method based on a deep-eutectic solvent comprises the following steps: firstly, crushing and grinding a plant to be extracted to obtain fine powder, then heating and uniformly mixing choline chloride, modified amino acid and lewis acid to prepare the deep-eutectic solvent, then mixing the fine powder, the deep-eutectic solvent and water according to a certain solid-to-liquid ratio, and uniformly mixing to obtain the plant extract based on the deep-eutectic solvent. And carrying out ultrasonic extraction at a certain temperature to obtain an extracting solution, and finally purifying. According to the method, the saponin component in the soapberry can be effectively extracted, the saponin yield is high, and the purity is high.

The invention discloses a method for extracting and purifying salidroside from salidroside fermentation liquor, which comprises the following steps: (1) adding tert-butyl alcohol into the salidroside fermentation liquor, filtering, and collecting clear liquid; (2) concentrating the clear solution obtained in the step (1) under reduced pressure to obtain a concentrated solution, adding petroleum ether for extraction, and collecting a water phase; (3) adding ammonium sulfate into the water phase obtained in the step (2), uniformly mixing, adjusting the pH to be acidic, adding ethyl acetate for extraction, and collecting an organic phase; and (4) concentrating the organic phase obtained in the step (3) under reduced pressure, and drying. Through a solvent polarity gradient matching technology and a salting-out enhanced phase separation mechanism, the structural stability of the salidroside is guaranteed, meanwhile, the extraction and purification technological process is simplified, the separation efficiency is remarkably improved, the purity and yield of the salidroside obtained through extraction and purification with the method are both larger than or equal to 90%, and the method is suitable for industrial production. The extraction and purification process provided by the invention is also provided with a solvent recovery system, and the efficiency and economy of industrial production are both considered.

The invention provides a pulullan refining method, which belongs to the technical field of biology, and specifically comprises the following steps: fermenting a microbial strain for producing pulullan to obtain fermentation liquor, and sterilizing and decoloring the fermentation liquor to obtain a crude pure extracting solution; carrying out a membrane process and a resin process on the obtained crude pure extracting solution to obtain a refined pure solution; the pore diameter of the membrane material is 500-1000Da; according to the method disclosed by the invention, it is found for the first time that the pullulan is extracted and purified by using a small-aperture nanofiltration membrane and is matched with resin adsorption, so that the purification effect can be achieved, the purity of the product is improved, and the yield of the product is also obviously improved.

The invention provides a method for detecting the content of organic acid in super-heavy oil, which comprises the following steps of: 1) separating the super-heavy oil: centrifuging by using a high-speed centrifuge, collecting and combining an upper crude oil phase; 2) organic acid extraction: extracting with normal hexane-cyclohexane for multiple times, and extracting with dichloromethane to obtain an organic acid component; the method comprises the following steps of 1, extracting the organic acid in the super-heavy oil, 2, extracting the organic acid in the super-heavy oil, 3, purifying the organic acid through a chromatographic column to obtain a purified organic acid component, and 4, detecting the organic acid through an ultra-high performance liquid chromatography-mass spectrometer.The organic acid in the super-heavy oil is extracted and purified through super-heavy oil separation, organic acid extraction and organic acid purification, the chromatographic column is not polluted, and detection is accurate.

The invention discloses a preparation process of an ovateleaf holly bark total saponin bulk drug, and particularly relates to the field of traditional Chinese medicine extraction and purification, the preparation process comprises the following steps: S1, extraction: crushing an ovateleaf holly bark medicinal material, and carrying out reflux extraction by using an ethanol aqueous solution as a solvent to obtain an ovateleaf holly bark crude extract; and S2, purification: carrying out solid-liquid separation on the crude ovateleaf holly bark extract, and purifying the filtrate through a recrystallization process and a macroporous resin adsorption process in sequence to obtain the ovateleaf holly bark total saponin bulk drug. According to the invention, an effective component group of ovateleaf holly bark for treating ulcerative colitis is taken as a target, the effective component group comprises syringin, pedunculoside and ziyuglycoside II, and a complete extraction-purification integrated process is constructed; through an orthogonal test, a comprehensive weighted scoring method is combined, and extraction parameters are scientifically optimized, so that the transfer rate of the three functional components is maximized, the extraction efficiency is high, and the pertinence is extremely high.

The invention provides a method for detecting saponin substances in American ginseng, and relates to the technical field of biological analysis, the detection method comprises the following steps: taking American ginseng, grinding the American ginseng into powder, sieving, and carrying out microwave-assisted extraction to obtain an extracting solution; then carrying out solid-phase extraction and purification on the extracting solution to obtain a purified solution; carrying out fluorescence derivatization on the purified liquid; and finally, carrying out HPLC-FLD analysis. According to the method disclosed by the invention, a microwave-assisted extraction-solid-phase extraction and purification combined fluorescence derivatization HPLC (High Performance Liquid Chromatography) technology is adopted, so that the defects of lengthy pretreatment and insufficient sensitivity in the traditional technology are overcome, and the dependence on scarce standard substances is also reduced.

The application discloses a kind of uranium nuclide automatic extraction device and method, wherein, device includes dissolving target device, acid regulator, extractor, purifier, waste liquid tank, product tank, control system and shielding box, dissolving target device, acid regulator, extractor, purifier, waste liquid tank, product tank are connected by conveying pipe and valve, liquid conveying power is provided by peristaltic pump, control system is set in shielding box, and relevant components in shielding box are electrically connected.The application also describes a method for extracting a uranium nuclide solution substantially free of impurities from an irradiated thorium target material.The uranium nuclide automatic extraction device of the present application can achieve rapid and efficient extraction and purification of uranium nuclides from irradiated thorium target material.The device has the characteristics of simple structure, compactness and automation operation, which greatly improves the production efficiency and reduces the radiation dose received by the operator.

The wastewater treatment system according to an embodiment of the present invention is used for treating wastewater containing a sodium sulfate solution, and comprises: an electrodialysis device for separating the sodium sulfate solution of the wastewater into a first solution containing sulfate ions, a second solution containing sodium ions, and circulating wastewater from which sodium sulfate has been removed; a first membrane distillation device that extracts purified water from the first solution in order to concentrate the first solution; and a second membrane distillation device that extracts purified water from the second solution in order to concentrate the second solution. According to the wastewater treatment system disclosed by the embodiment of the invention, salt compounds and other harmful ions in the wastewater can be selectively separated through the electrodialysis device so as to effectively separate a high-concentration ionic solution. Therefore, not only can substances after wastewater treatment be recycled to the maximum extent, but also the environmental harmfulness of salt compounds can be greatly reduced.

The invention relates to the technical field of medicine impurity purification, in particular to a separation and purification method of a high-purity urapidil impurity compound. Comprising the following steps: 1) performing vacuum concentration on urapidil mother liquor to obtain mother liquor concentrate M, adding dichloromethane, then adding 100-200-mesh silica gel, and performing vacuum concentration at 35-45 DEG C until the mother liquor concentrate M is dry; (2) weighing 200-300-mesh silica gel, adding dichloromethane, uniformly mixing, filling into a chromatographic column, and compacting for later use; (3) loading the vacuum concentrate in the step (1) into a chromatographic column, adding anhydrous sodium sulfate, and adding eluent for gradient elution; 4) collecting the eluent when the last eluent is used for elution, and performing reduced pressure concentration at 40-50 DEG C; after the concentration is finished, the urapidil impurity compound M1 can be obtained. According to the method for directly extracting and purifying the impurity compound from the mother liquor for preparing urapidil, the process is simpler, the purity of the purified impurity compound is 96-100%, and the accuracy and precision of the verification and detection method can be improved.

The invention provides a method for intensively extracting and purifying 5N-grade high-purity cobalt and nickel through ionic liquid, and belongs to the technical field of non-ferrous metal separation and purification. According to the method, the specific ionic liquid is selected as the extraction medium, matched with the synergistic extraction agent and combined with the micro-channel reactor to achieve efficient mass transfer, the Co / Ni and Mn / Cu separation coefficient can be remarkably increased, the ionic liquid recovery rate is increased, the 5N-grade high-purity cobalt-nickel product is finally obtained, and the process is easy for large-scale continuous production and has a wide application prospect. The defects of poor Mn and Cu impurity separation selectivity, high solvent loss rate and low process efficiency in the existing Co / Ni paragenic ore extraction and purification process are overcome. And meanwhile, the impurity separation selectivity is remarkably improved, the solvent loss is reduced, the process is efficient and environmentally friendly, the method is suitable for large-scale industrial production of high-purity cobalt and nickel, and key raw material guarantee is provided for the fields of new energy, electronic information and the like. The result of the embodiment shows that the cobalt and the nickel obtained by the invention both meet the 5N-level requirement.

The application relates to the technical field of preparation of shuganbin, and discloses a shuganbin rapid extraction and purification device and process.The device comprises a lower jar body, a filter disc, an assembling rod rotatably installed on the filter disc, an extrusion roller for extruding and dispersing raw materials rotatably installed on the assembling rod, and a transmission assembly; after the filter disc is lifted, the assembling rod is docked with the stirring shaft through the clamping assembly, the stirring shaft rotates to drive the assembling rod to rotate synchronously, the transmission assembly drives a plurality of dispersing wheels to rotate, the plurality of dispersing wheels can disperse the raw materials on the filter screen, then the extrusion roller rotates to the raw materials dispersed by rolling, the solution infiltrated in the shuganpi raw materials after filtration can be extruded, the solution most fully combined with the shuganpi raw materials can be fully extracted, the problem that the solution is wasted and the shuganbin cannot be fully extracted due to the direct removal of the solution after filtration is avoided, and the extraction efficiency of the shuganbin is greatly improved.

The invention discloses an extraction process of chaste tree twig oil in the technical field of plant essential oil extraction. An ethanol aqueous solution is adopted for extraction, ultrasonic treatment and a composite enzymolysis technology are combined, the chaste tree twig cell structure is effectively destroyed, and the grease extraction rate is remarkably increased. And then selectively adsorbing impurities in the extracting solution by using a specially-made cobalt ferrite-deep eutectic solvent-biochar magnetic composite material, and realizing rapid separation through an external magnetic field. And the alkalescent ethanol water solution is adopted for elution, so that the structure stability of the deep eutectic solvent is protected while impurities are effectively desorbed. And finally, carrying out rotary evaporation and column chromatography refining to obtain the high-quality vitex negundo oil. The process integrates various extraction and purification technologies, has the advantages of high extraction efficiency, mild operation conditions, small environmental pollution and the like, and is particularly suitable for efficient extraction and refining of the vitex negundo oil.

The invention discloses an effective part of a pulse-activating decoction as well as an extraction and purification method and application thereof, and the extraction and purification method comprises the following steps: taking red ginseng, radix ophiopogonis and schisandra chinensis as raw materials, adding water for extraction, and freeze-drying to obtain freeze-dried powder; dissolving the freeze-dried powder with distilled water, loading the dissolved powder on a macroporous adsorption resin column, carrying out gradient elution with water and a 20% ethanol aqueous solution in sequence, and separating fractions Fr1-Fr8; the chromatographic characteristics of each fraction are analyzed by using a C18 chromatographic column through a liquid chromatograph, the fractions with the same chromatographic characteristics are combined, the fractions Fr1-Fr4 are combined into a component yf-1, the fraction Fr5 is combined into a component yf-2, and the component yf-2 is the effective part of the pulse-activating decoction. According to the effective part disclosed by the invention, toxic parts are removed, so that H9c2 cell injury caused by adriamycin can be more effectively and stably protected with lower toxicity, and the application of the component yf-2 in preparation of medicines for treating myocardial cell injury is prompted.

The invention belongs to the field of analytical chemistry and food safety detection, and discloses a fish oil plasticizer liquid chromatography-tandem mass spectrometry detection method, which is characterized in that a target molecule is structurally modified through a specific chemical derivatization reagent, and the electrospray ionization efficiency is remarkably improved, so that a mass spectrometry response signal is enhanced; solid-phase extraction purification and high-performance reversed-phase chromatography separation are combined, so that effective removal of matrix interference and synchronous detection of various plasticizers are realized; the method does not need a complex solvent system, and is simple in operation flow and good in reproducibility; the detection limit reaches ppb level, the linear range is wide, the method is suitable for conventional and high-throughput detection of the plasticizer in fish oil and fish oil products, and a sensitive and reliable analysis tool is provided for quality safety assessment of related products.

Disclosed is an extraction and purification method for biosynthetic squalene. The present invention pertains to the technical field of squalene extraction. In the present invention, the synergy between antioxidant addition, solvent pretreatment, and process condition control can reduce the peroxide value of the extracted finished product. The peroxide value can be as low as 1 meq / kg or lower, thereby meeting the pharmaceutical-grade peroxide value standard, and both the content and purity can reach 99% or higher. In addition, the use of a single organic solvent for extraction and chromatography avoids the difficult recovery problem with the cross-use of multiple solvents; the single organic solvent can be reused repeatedly. The total extraction yield is as high as 90%. The method is suitable for industrial continuous production.

The invention discloses a method for preparing high-purity phycocyanin by coupling supercritical extraction with aqueous two-phase extraction, which comprises the following steps: pretreatment of raw materials: pretreating spirulina powder, and centrifuging to obtain a phycocyanin crude extract; carrying out supercritical CO2 impurity removal: filling the phycocyanin crude extract obtained in the previous step into a supercritical extraction device, carrying out static extraction, and collecting an orange extract rich in carotenoid; then pumping ethanol as a carrying agent, carrying out dynamic extraction, and respectively collecting chlorophyll components and wet algae residues; and two-aqueous-phase extraction purification and post-treatment: purifying the treated wet algae residues by adopting a two-aqueous-phase extraction method, dialyzing by adopting a dialysis bag, and carrying out vacuum freeze drying to obtain a pure product. The phycocyanin distribution selectivity is remarkably improved, and the method is environmentally friendly and efficient; the time is shortened by about 93% compared with that of a traditional method, the phycocyanin purity is stable and is larger than or equal to 4.0, the production cost is greatly reduced, and the method is suitable for large-scale production.

The invention provides a topiramate cleaning chemical residue detection method and kit.The method comprises the steps that sampling is conducted in a specified area to obtain a sample, and the sampling step comprises wiping sampling and / or washing sampling; the method comprises the following steps: adding a mixed extraction solvent into a sample for extraction, filtering an extraction solution, adjusting the extraction solution to alkalescence, and adding a derivatization reagent for reaction to obtain a purified solution to be detected; the purified liquid to be detected is injected into a chromatographic detection system for quantitative detection, a detection result is output, and the detection step comprises chromatographic separation and signal response analysis. Accurate detection and limit judgment on topiramate residues on the surface of equipment are realized through standard sampling, extraction purification and derivatization enhanced response and in combination with chromatographic quantitative analysis, so that collinear production pollution and cross contamination risks of medicines are effectively controlled.

The present application relates to the technical field of traditional Chinese medicine extract, and particularly relates to a method for extracting artabotrine from atractylodes lancea, comprising the following steps: S1, subjecting atractylodes lancea to enzymatic hydrolysis treatment to obtain an enzymatic hydrolysis mixture; S2, subjecting the enzymatic hydrolysis mixture to aqueous two-phase extraction treatment to obtain an extraction liquid; the aqueous two-phase extraction treatment adopts a mixed solution composed of ethanol with a mass fraction of 8-13% and sodium dihydrogen phosphate with a mass fraction of 25-30%; the solid-liquid ratio of the enzymatic hydrolysis mixture and the mixed solution is 1g:(16-24)mL; S3, subjecting the extraction liquid to concentration treatment to obtain an extract, then dissolving the extract with petroleum ether and performing gradient elution through liquid chromatography to obtain artabotrine. The extraction and purification method of the present application can effectively, mildly and efficiently extract artabotrine from atractylodes lancea, and has high extraction purity, high yield, simple process flow, short time consumption and low production cost.

The invention provides a method for uniformly preparing an aluminum-scandium alloy, and relates to the technical field of aluminum-scandium alloy preparation. The method comprises the following steps: carrying out alkaline leaching on the aluminum-scandium enrichment to obtain a sodium metaaluminate solution and scandium-rich slag; performing a seed precipitation method on the sodium metaaluminate solution to obtain aluminum hydroxide; the scandium-rich slag is subjected to normal-pressure acid leaching through sulfuric acid, and scandium-rich liquid is obtained; extracting and purifying the scandium-rich solution to obtain a pure scandium sulfate solution; dissolving aluminum hydroxide in a pure scandium sulfate solution according to a certain aluminum-scandium ratio to obtain an aluminum scandium sulfate solution; adding sodium hydroxide into the aluminum scandium sulfate solution for precipitating aluminum and scandium to obtain aluminum scandium hydroxide; calcining the aluminum hydroxide scandium at high temperature to obtain aluminum oxide scandium; cryolite is added into the aluminum oxide scandium, and the aluminum-scandium alloy is obtained through high-temperature electrolysis. The method has the advantages of efficient and simple process flow, low investment, low cost, no discharge of a large amount of wastewater, greenness, low carbon, environmental protection and the like, has huge social and economic values, and is beneficial to industrial large-scale application and popularization.

This invention relates to the field of wastewater treatment and zero-discharge technology, providing an in-situ coupled synergistic system for hardening removal and resource utilization. The system includes a hardening removal process section and a concentration and desalination process section connected in sequence. The hardening removal process section removes hardness from water, while the concentration and desalination process section extracts and purifies inorganic salts from the water, with the inorganic salts serving as the desorbent and / or precipitant in the hardening removal process. The hardening removal process section includes a hardness adsorption module and a desorption module. The hardness adsorption module extracts hardness from the incoming water. The desorption module is connected to the hardness adsorption module and desorbs the hardness adsorption module, restoring the adsorption function of the adsorbent in the hardness adsorption module. The hardness adsorption module consists of hardness adsorption components filled with a hardening adsorbent, and these components have a columnar structure. This invention couples adsorption technology with desalination and purification technology, synergizing with particle membrane filtration technology to shorten the process flow and achieve waste resource utilization.

This invention discloses a method for extracting and purifying lutein extract based on a composite solvent system, specifically relating to the field of natural product separation and extraction. The method involves mixing a crude extract containing lutein esters from marigold with a first composite solvent system. This allows lutein esters, oils, and resinous impurities in the crude extract to enter the liquid phase. Waxes are then precipitated by lowering the system temperature, resulting in a dispersion system containing the precipitated waxes. The first composite solvent system consists of a first organic solvent and a second organic solvent. By first introducing lutein esters, oils, and resinous impurities into the liquid phase in the first composite solvent system, then allowing the waxes to precipitate and separate, and subsequently transferring the lutein esters to a lutein ester-enriched phase, lutein can be separated from major accompanying impurities before entering the saponification and purification processes. This relatively reduces the burden on subsequent processes and alleviates issues such as slow stratification, increased entrainment, and batch fluctuations.

The utility model discloses a perfluorinated sulfonic acid resin refining and purifying device, which belongs to the technical field of purification and comprises a purifying tank, a top cover is arranged at the upper end of the purifying tank, a feed port is arranged on the top cover, a support is mounted on the top cover, a stirring motor is fixed on the support, a stirring frame is arranged in the purifying tank, and an ultrasonic interface sensor is mounted on the top cover. By arranging the upper-layer light liquid extraction structure, a servo motor, a lead screw and other structures, the height of an installation assembly can be adjusted, then a lifting suction pipe is driven to move, the end of the lifting suction pipe can be ensured to be close to the interface of two kinds of extracted and purified liquid in cooperation with an ultrasonic interface sensor, the suction accuracy is ensured, and the extraction efficiency is improved. The screw rod and the adjusting rod have the self-locking effect, the stability after adjustment can be guaranteed, the suction effect is guaranteed, the lifting suction pipe is installed through the installation assembly, and the initial height of the lifting suction pipe can be adjusted.

The invention relates to the field of radix cynanchi bungei polysaccharide extraction and purification, and particularly discloses a coastal radix cynanchi bungei polysaccharide extraction and purification device which comprises a stirring and mixing tank, the bottom of the stirring and mixing tank is connected with a first conveying pipeline, the other end of the first conveying pipeline is connected with a vibration screening tank, and the bottom of the vibration screening tank is connected with a second conveying pipeline. Impurity separation is enhanced through multi-stage vibration screening and anti-blocking design, the vibration screening tank is of a layered filtering structure with a first-stage screen plate and a second-stage screen plate, high-frequency vibration is matched, and the multi-stage vibration screening and anti-blocking design is adopted, so that the multi-stage vibration screening and anti-blocking design is achieved, and the multi-stage vibration screening and anti-blocking design is achieved. Large-particle residues and fine impurities can be removed step by step; the scraping plate cleans the sieve plate, the blockage rate of sieve pores is reduced, the subsequent purification burden is relieved, the purity of crude extract is improved, and the problems of poor screening effect and easy blockage are solved; the purification reaction is accelerated through the coaxial reverse paddle stirring mechanism, the mixing time is shortened, and the problem of low purification efficiency is solved.

The application belongs to an extraction and purification process, and discloses a capsaicin extraction and purification method. The method comprises the following steps: mixing crushed chili powder with a composite enzyme, dispersing the mixture in a plasma active eutectic solvent, and simultaneously performing enzymolysis, extraction and purification; after extraction, centrifuging and collecting the supernatant, and freeze-drying after concentration. The application performs targeted extraction and purification by preparing and applying a plasma active eutectic solvent with high selectivity for capsaicin, and the purity of the extract is high. The yield and purity of capsaicin extracted by the method are 222.04% and 36.36% higher than those of 70% (v / v) ethanol extraction, respectively, and the use of a large amount of organic reagents is avoided, which meets the requirements of green chemistry and is conducive to the industrialized production and application of capsaicin.

The utility model relates to an extraction and purification device for green synthesis of chloramine, which belongs to the technical field of extraction equipment and comprises an extraction tank, a feed pipe, an extraction agent inlet pipe, a stirring shaft, a stirring impeller, a stirring motor, a three-way pipe I, a material receiving tank, a recovery tank, a discharge pipe, a circulating pipe, a circulating pump and a premixing box, a distribution cone disc is fixed to the lower portion in the premixing box, distribution holes are evenly formed in the distribution cone disc, the other end of the circulating pipe is connected to the top of the premixing box, and an outlet of the circulating pipe faces the top of the distribution cone disc. Separating liquid can enter the recycling tank after primary extraction, then the separating liquid is pumped into the extraction tank again through the circulating pipe and the circulating pump for next extraction, automation is achieved, labor intensity is reduced, efficiency is improved, in addition, a mixture and an extracting agent which are fed initially, and the circulating separating liquid and the extracting agent can be distributed and premixed in the premixing box, and the efficiency is improved. Mixing is accelerated and the extraction efficiency is improved.

The utility model discloses an arctiin extracting and purifying device which comprises a processing barrel, a fixing box, a solution separating mechanism, a lifting assembly, a squeezing assembly, a connecting frame, a fixing block and an extracting and purifying mechanism, and the solution separating mechanism comprises a threaded rod, a driving motor, a guide rod, a nut seat, a connecting rod, a rotating shaft support, a squeezing disc, a filter screen, a telescopic air cylinder and a supporting disc. The extraction and purification mechanism comprises a purification barrel, a fixed table, a support plate and a filter layer support table. The utility model belongs to the technical field of arctiin purification, a nut seat can be driven to move by starting a driving motor, so that a squeezing disc is conveniently driven to descend to sufficiently squeeze crushed burdock on a filter screen to thoroughly separate out a solution, and a telescopic cylinder is started to extend to drive a supporting disc to be separated from the filter screen, so that the separated solution can flow out conveniently; different types of filter layers are fixedly placed on each group of filter layer support table, so that arctiin can be conveniently and finely extracted and purified from a flowing burdock precipitation solution.

The invention provides a method for determining oxyfenthion, oxyfenthion sulfone and oxyfenthion sulfoxide in fruits and vegetables. The method comprises the following steps: sampling; the pretreatment comprises the following steps: adding a 1% glacial acetic acid solution into the to-be-detected sample, and carrying out vortex standing; then adding acetonitrile and an internal standard solution, and carrying out vortex oscillation to obtain a dissolved solution; adding mixed powder of anhydrous magnesium sulfate and buffer salt, immediately shaking and dispersing, and sequentially carrying out oscillation, ice bath and centrifugal treatment; taking the primary supernate, putting the primary supernate into a dispersive solid-phase extraction purification tube filled with a purification material, and sequentially carrying out vortex treatment, oscillation treatment and centrifugal treatment; taking the second supernatant, and performing water bath nitrogen blowing concentration to obtain a redissolved sample; then adding acetonitrile into the redissolved sample to a constant volume, uniformly mixing and filtering to obtain an on-machine test solution; and putting the loading test solution into a high performance liquid chromatography tandem mass spectrometer for qualitative and quantitative detection and analysis. According to the method, the content of oxyfenthion, oxyfenthion sulfone and oxyfenthion sulfoxide in fruits and vegetables can be accurately measured, and the blank of national standard methods is filled.