128 results about "Extraction Purification" patented technology

The invention relates to the technical field of preparation of silibinin, and discloses a rapid extraction and purification device and process for silibinin. A filter disc; the assembling rod is rotationally mounted on the filtering disc, and an extrusion roller used for extruding the scattered raw materials is rotationally mounted on the assembling rod; and a transmission assembly. After the filter disc ascends, the assembling rod is in butt joint with the stirring shaft through the clamping assembly, the stirring shaft rotates to drive the assembling rod to synchronously rotate, the transmission assembly drives the scattering wheels to rotate, the scattering wheels can scatter raw materials on the filter screen, and then the extrusion roller rotates to the position to roll the scattered raw materials; according to the silybin extraction device, a solution infiltrated in a silybum marianum raw material can be extruded after filtration, a solution which is most fully fused with the silybum marianum raw material is fully extracted, the problems that the solution is wasted due to the fact that the solution is directly removed after filtration, and silybin cannot be fully extracted are solved, and the extraction efficiency of the silybin is greatly improved.

The invention relates to the technical field of polysaccharide extraction, in particular to a dendrobium huoshanense protocorm polysaccharide extraction and purification method which comprises the following steps: cleaning dendrobium huoshanense protocorm, freeze-drying, crushing and sieving to obtain raw material powder; measuring initial raw material characteristic parameters of the raw material powder, and setting initial extraction parameters according to the initial raw material characteristic parameters; mixing the raw material powder with an extraction solvent, monitoring the concentration of polysaccharide in an extracting solution in real time in the extraction process, and calculating an extraction activity index; comparing the extraction activity index with a preset extraction activity threshold value, and adaptively adjusting extraction parameters according to a comparison result; the extracted extracting solution is purified, and the impurity characteristics of the extracting solution are monitored in real time in the purification process; the purification parameters are adaptively adjusted based on the characteristics of the impurities in the extracting solution obtained through real-time monitoring. The method can improve the polysaccharide yield stability and improve the product quality and consistency.

The invention discloses a method for extracting erythrinine from beautiful millettia roots by using a deep-eutectic solvent and a purification process. Wherein the optimal process for extracting the erythrinine is as follows: LA-Fruu (1: 1) with the water content of 40% is used under the conditions that the ultrasonic temperature is 80 DEG C, the ultrasonic time is 40 minutes and the liquid-material ratio is 20: 1, and the extraction amount of the erythrinine can reach 3.1495 + / -0.0690 mg / g; the optimal purification process is as follows: DM132 type macroporous resin is used, the eluent is 50% ethanol, the elution flow rate is 2.5 BV / h, the height-diameter ratio of a chromatographic column is 21: 1, the elution volume is 4BV, and the recovery rate of erythrinine is 89.53 + / -3.23%. According to the invention, a deep eutectic solvent and macroporous resin combined technology is established, the erythrinine is extracted and purified from the millettia specisoa champ in a high-efficiency, rapid, green and environment-friendly manner, and a green and efficient way is provided for the extraction and purification of the erythrinine.

The invention provides a method for extracting and purifying long-chain dicarboxylic acid from fermentation liquor, which comprises the following steps: (1) controlling the pH value of the fermentation liquor to be less than or equal to 5 and the density to be 0.9-1.16 g / cm < 3 > to obtain a first solid-liquid mixture; (2) centrifuging the first solid-liquid mixture to obtain a second solid-liquid mixture of which the content of long-chain dibasic acid is greater than that of other solid substances and a third solid-liquid mixture of which the content of long-chain dibasic acid is smaller than that of long-chain dibasic acid in the second solid-liquid mixture; and (3) separating the second solid-liquid mixture to obtain the long-chain dicarboxylic acid. The method is simple to operate, low in equipment requirement and investment and high in treatment efficiency. And most thalli and impurities in the fermentation liquor are removed through centrifugal separation. And the obtained product has high purity and high product yield.

The embodiment of the invention discloses a separation method for extracting phycocyanin from spirulina platensis through eutectic aqueous two-phase extraction, relates to the technical field of extraction and purification of phycocyanin, and aims at solving the problems that in the prior art, when phycocyanin is extracted through a eutectic aqueous two-phase system, the cost is high, the raw material handling capacity is low, and the salt concentration is high. The separation method comprises the following steps: extracting spirulina platensis powder by adopting a CaCl2 solution, and carrying out freeze thawing wall breaking treatment and centrifugal treatment on the extracted spirulina platensis powder to obtain a crude extract containing phycocyanin; the method comprises the following steps: carrying out ultrafiltration desalting treatment on a crude extract containing phycocyanin, adding a deep eutectic solvent and a sodium potassium tartrate tetrahydrate solution, uniformly mixing to obtain an aqueous two-phase system, adjusting the pH value, uniformly mixing, vortex, standing for phase separation, centrifuging for promoting phase separation, separating an upper phase, and carrying out ultrafiltration treatment on the upper phase to obtain filtrate of the deep eutectic solvent component and a concentrated phycocyanin solution; the concentrated phycocyanin solution is subjected to freeze drying treatment, and phycocyanin powder is obtained.

The invention discloses an extraction purification deep impurity removal method for a nickel sulfate solution, and belongs to the technical field of nickel sulfate solution purification, and the extraction purification deep impurity removal method comprises the following steps: carrying out saponification treatment on a P204 extraction agent by adopting a sodium hydroxide solution to obtain a saponified organic phase P204, mixing the saponified organic phase P204 with a nickel sulfate water phase, stirring and standing to obtain a nickel sulfate solution; the method comprises the following steps: separating an organic phase from a water phase, mixing the organic phase with sulfuric acid, stirring, standing, separating out a nickel sulfate solution, concentrating the nickel sulfate, and recrystallizing to obtain the high-purity nickel sulfate with the purity of 99.4-99.9%. According to the method, the battery-grade nickel sulfate product with the purity as high as 99.9% can be obtained, the requirement of HG / T2824-2009 first-grade products is met, and the method is particularly suitable for purification of crude nickel sulfate generated in the copper ore smelting process and preparation of the battery-grade nickel sulfate.

The invention discloses a mussel mucoprotein extraction and purification method, which comprises: 1) taking mussel foot glands, adding an extraction liquid 1, soaking, homogenizing, precipitating, taking the precipitate, adding an extraction liquid 2, re-suspending, homogenizing, carrying out centrifugal filtration, and taking the filtrate to obtain an extract; and 2) purifying the extract obtained in the step 1). The mussel mucoprotein obtained by extracting through a specific extracting solution and then adopting a specific purification method is high in protein and dopa content, can be compatible with pharmaceutical auxiliary materials such as anionic polymer components carbomer, sodium hyaluronate and sodium carboxymethyl cellulose, is used for preparing medicines or skin care products, and is wide in application prospect.

The invention relates to the technical field of fermentation extraction, and particularly provides a PHA extraction and purification method, a PHA product and application, the PHA extraction and purification method comprises the following steps: (1) adding a surfactant I into a PHA fermentation broth, stirring while keeping the temperature, adding alkali until the feed liquid is alkaline, and carrying out mechanical wall breaking treatment to obtain a first feed liquid; (2) adding lysozyme into the first feed liquid, keeping the temperature, adding alkali until the feed liquid is alkaline, adding alkaline protease, keeping the temperature, and obtaining second feed liquid; and (3) adding a surfactant II into the second feed liquid, stirring and washing, wherein the surfactant II comprises SDS (sodium dodecyl sulfate), sodium lauryl sulfate and lauryl glucoside. The PHA product obtained by adopting the PHA extraction and purification method has excellent purity and whiteness, so that the PHA product has a wider application prospect, and the method is simple in process, convenient to operate and low in cost.

The invention relates to the technical field of chemical purification, and particularly discloses an extraction and purification method of tetraacetyl phytosphingosine. The invention provides an extraction and purification method of tetraacetyl phytosphingosine. The extraction and purification method comprises the following steps: sample treatment, ethanol extraction, C8 chromatography, PA308 chromatography and PS-5 chromatography. Wherein an eluent used in the steps of C8 chromatography and PA308 chromatography is a 50-60v / v% acetonitrile-water solution; an eluent used in the PS-5 chromatography step is an acetonitrile-water solution with the concentration of 65-85v / v%. According to the extraction and purification method of the tetraacetyl phytosphingosine provided by the invention, the tetraacetyl phytosphingosine in thalli can be fully extracted, and the purity of the tetraacetyl phytosphingosine in the obtained tetraacetyl phytosphingosine extract is greater than or equal to 95%.

The invention provides an extraction and purification method of tetraacetyl phytosphingosine. Comprising the following steps: pretreatment and plate frame filtration of tetraacetyl phytosphingosine fermentation liquor, flash evaporation drying of a thallus wet filter cake, microwaves of bacterial powder and reflux extraction of a solvent, reverse extraction, enrichment and impurity removal of an extracting solution, preparation of a crude extract, primary crystallization of the crude extract by a polar solvent, alternate solvent crystallization of a crystallized primary product and the like. The tetra-acetyl phytosphingosine is extracted from the bacterial powder obtained by flash evaporation and drying after thallus separation by adopting a microwave and extraction solvent condensation reflux technology, and the method has the advantages of small solvent dosage, high extraction speed and high extraction yield. Besides, silica gel column chromatography separation and purification are replaced by means of reverse extraction, enrichment and impurity removal and alternate solvent crystallization, use of a mixed solvent and generation of silica gel solid waste are avoided, the purposes of saving cost and reducing environmental pollution can be achieved at the same time, and the method has wider application prospects and better industrial production potential.

The utility model relates to plant extraction technical field discloses a kind of plant exosome extraction and purification machine, including bottom plate, the left side fixedly connected with bracket of bottom plate, the output end fixedly connected with multiple blades of motor one, the outer wall of wall breaking box is connected with filter box, the inner wall fixedly connected with multiple activated carbon plate of filter box, the bottom of filter box is connected with burette, the outer wall fixedly connected with piston of burette, the top fixedly connected with fixed mechanism of bottom plate, the fixed mechanism is used for fast installation.In the utility model, open motor one, blade breaks the wall of plant, activated carbon plate filters out residue and pigment, piston adjusts the flow rate and dosage of juice, let juice drop into mixed test tube, simplify the operation of early separation filtering of plant solvent, reduce the time cost of experiment, improve the efficiency of extraction and purification, increase practicality, expand the scope of application.

The invention belongs to the technical field of plant active component extraction and purification, and particularly provides a plant extract purification method based on a deep-eutectic solvent. A plant extract purification method based on a deep-eutectic solvent comprises the following steps: firstly, crushing and grinding a plant to be extracted to obtain fine powder, then heating and uniformly mixing choline chloride, modified amino acid and lewis acid to prepare the deep-eutectic solvent, then mixing the fine powder, the deep-eutectic solvent and water according to a certain solid-to-liquid ratio, and uniformly mixing to obtain the plant extract based on the deep-eutectic solvent. And carrying out ultrasonic extraction at a certain temperature to obtain an extracting solution, and finally purifying. According to the method, the saponin component in the soapberry can be effectively extracted, the saponin yield is high, and the purity is high.

The rosemary essential oil extracting and purifying device comprises a tank body, the lower end of the front side of a collecting box is fixedly connected with an installing groove, splicing inserting grooves are formed in the two sides of the inner wall of the installing groove, splicing inserting blocks are installed on the inner walls of the splicing inserting grooves in an inserted mode, and an essential oil collecting box is fixedly connected between the splicing inserting blocks; a rotating motor is inserted in the center of the upper end of the tank cover, a rotating rod is installed at the output end of the rotating motor, a cutting tool bit is fixedly connected to the outer wall of the rotating rod, and a heating mechanism is installed at the lower end of the inner wall of the tank body. According to the rosemary essential oil extraction and purification device, raw materials can be efficiently smashed through the cutting tool bit, the distillation and extraction effect is better, multi-group condensation and collection can be conducted through the condensation box, the conductor refrigerator and the condensation guide pipe which are annularly distributed, oil-water separation can be conducted through the water outlet and the essential oil outlet, and the efficiency is high. Filtering treatment can be performed through the filtering block, so that the extraction effect is better, and the use is more convenient.

The invention discloses a method for extracting and purifying salidroside from salidroside fermentation liquor, which comprises the following steps: (1) adding tert-butyl alcohol into the salidroside fermentation liquor, filtering, and collecting clear liquid; (2) concentrating the clear solution obtained in the step (1) under reduced pressure to obtain a concentrated solution, adding petroleum ether for extraction, and collecting a water phase; (3) adding ammonium sulfate into the water phase obtained in the step (2), uniformly mixing, adjusting the pH to be acidic, adding ethyl acetate for extraction, and collecting an organic phase; and (4) concentrating the organic phase obtained in the step (3) under reduced pressure, and drying. Through a solvent polarity gradient matching technology and a salting-out enhanced phase separation mechanism, the structural stability of the salidroside is guaranteed, meanwhile, the extraction and purification technological process is simplified, the separation efficiency is remarkably improved, the purity and yield of the salidroside obtained through extraction and purification with the method are both larger than or equal to 90%, and the method is suitable for industrial production. The extraction and purification process provided by the invention is also provided with a solvent recovery system, and the efficiency and economy of industrial production are both considered.

The utility model provides a full-automatic plasmid purification instrument which comprises a base, an oscillation device, a reagent strip device, a first moving device and a liquid injection device are arranged on the base, a washing area and an elution area are arranged on the reagent strip device, and the liquid injection device is used for injecting reagents into hole sites in the oscillation device; the first moving device is provided with a magnetic bar magnetic sleeve part, the magnetic bar magnetic sleeve part comprises a moving frame connected with the first moving device, the moving frame is provided with a liftable magnetic sleeve and a magnetic bar, and the magnetic bar can extend into the magnetic sleeve; the movable frame is further provided with a cutting device. When the escherichia coli plasmid DNA extraction and purification device is used for extracting and purifying escherichia coli plasmid DNA, the steps of liquid injection, oscillation, magnetic bead extraction, cleaning, elution and the like can be automatically completed, manual intervention is not needed in the whole extraction and purification process, the labor cost is saved, and the working efficiency is high; and the whole extraction and purification process does not need manual operation intervention, so that the risk that the reagent is polluted due to improper manual operation is reduced.

The invention discloses a twice crystallization method for extracting hesperidin, and belongs to the technical field of plant extraction. The method for extracting hesperidin comprises the following steps: cleaning and crushing a raw material rich in hesperidin; adding an impurity removal solvent, and carrying out impurity removal reaction; adding an extraction solvent into the raw materials subjected to impurity removal to carry out extraction reaction, wherein the extraction solvent is a mixed solution of low-level saturated monohydric alcohol with the volume percent of 40-80% and alkali with the mass percent of 0.3-1.0%; adding acid into the extracting solution to adjust the pH value to 7.5-8.5; carrying out a first crystallization reaction, adding acid into the residual mother liquor to adjust the pH value to 6.5-7.5, and carrying out a second crystallization reaction; the extraction, purification and recovery processes of the hesperidin are optimized through a two-time crystallization method of sequentially performing crystallization in an alcohol system and a water system. The method not only overcomes the defects of large wastewater discharge amount and complex process of the traditional extraction process, but also further improves the extraction yield of hesperidin, reduces the production cost, and makes the extraction process more efficient and environment-friendly.

The invention provides a pulullan refining method, which belongs to the technical field of biology, and specifically comprises the following steps: fermenting a microbial strain for producing pulullan to obtain fermentation liquor, and sterilizing and decoloring the fermentation liquor to obtain a crude pure extracting solution; carrying out a membrane process and a resin process on the obtained crude pure extracting solution to obtain a refined pure solution; the pore diameter of the membrane material is 500-1000Da; according to the method disclosed by the invention, it is found for the first time that the pullulan is extracted and purified by using a small-aperture nanofiltration membrane and is matched with resin adsorption, so that the purification effect can be achieved, the purity of the product is improved, and the yield of the product is also obviously improved.

The invention provides a method for detecting the content of organic acid in super-heavy oil, which comprises the following steps of: 1) separating the super-heavy oil: centrifuging by using a high-speed centrifuge, collecting and combining an upper crude oil phase; 2) organic acid extraction: extracting with normal hexane-cyclohexane for multiple times, and extracting with dichloromethane to obtain an organic acid component; the method comprises the following steps of 1, extracting the organic acid in the super-heavy oil, 2, extracting the organic acid in the super-heavy oil, 3, purifying the organic acid through a chromatographic column to obtain a purified organic acid component, and 4, detecting the organic acid through an ultra-high performance liquid chromatography-mass spectrometer.The organic acid in the super-heavy oil is extracted and purified through super-heavy oil separation, organic acid extraction and organic acid purification, the chromatographic column is not polluted, and detection is accurate.

The invention relates to the technical field of extraction and purification, and particularly provides an acerola cherry extract as well as a preparation method and application thereof, the preparation method comprises the following steps: crushing acerola cherries to obtain acerola cherry jam; squeezing the acerola cherry jam liquid to obtain juice, and filtering the juice through a non-woven fabric bag to obtain acerola cherry juice; adding a flocculating agent into the acerola cherry juice, stirring, standing, centrifuging, taking supernate, and carrying out vacuum concentration to obtain a concentrated solution; and adding ethanol into the concentrated solution for alcohol precipitation, collecting the alcohol precipitation clear solution, filtering, concentrating and drying to obtain the acerola cherry extract. The acerola cherry extract obtained by the method has higher vitamin C content and lower metal salt content, can be better applied to processing and production of wine bodies, and has a good application prospect.

The invention discloses a preparation process of an ovateleaf holly bark total saponin bulk drug, and particularly relates to the field of traditional Chinese medicine extraction and purification, the preparation process comprises the following steps: S1, extraction: crushing an ovateleaf holly bark medicinal material, and carrying out reflux extraction by using an ethanol aqueous solution as a solvent to obtain an ovateleaf holly bark crude extract; and S2, purification: carrying out solid-liquid separation on the crude ovateleaf holly bark extract, and purifying the filtrate through a recrystallization process and a macroporous resin adsorption process in sequence to obtain the ovateleaf holly bark total saponin bulk drug. According to the invention, an effective component group of ovateleaf holly bark for treating ulcerative colitis is taken as a target, the effective component group comprises syringin, pedunculoside and ziyuglycoside II, and a complete extraction-purification integrated process is constructed; through an orthogonal test, a comprehensive weighted scoring method is combined, and extraction parameters are scientifically optimized, so that the transfer rate of the three functional components is maximized, the extraction efficiency is high, and the pertinence is extremely high.

The utility model discloses an efficient biochemical fulvic acid extracting and purifying device which comprises a fixing frame, side plates are fixedly installed on the two sides of the fixing frame respectively, a power assembly is fixedly installed on one side of each side plate, a screening assembly is rotationally connected to one side of each power assembly, and an anti-falling assembly is fixedly installed on one side of the fixing frame. A feeding assembly is fixedly installed on the side, away from the anti-falling assembly, of the fixing frame. The anti-falling assembly comprises a fixed block and a sliding plate, the fixed block is fixedly mounted on one side of the fixed frame, and the sliding plate is connected to the inner side wall of the fixed block in a sliding manner. According to the efficient extraction and purification device for biochemical fulvic acid, by arranging the anti-falling assembly, a very good anti-falling function can be achieved in the screening process of biochemical fulvic acid, so that the biochemical fulvic acid is prevented from falling on the ground and being contaminated by dust, the inconvenience of manual secondary purification is reduced, unnecessary waste is reduced, and the use effect is better.

The invention provides a method for detecting saponin substances in American ginseng, and relates to the technical field of biological analysis, the detection method comprises the following steps: taking American ginseng, grinding the American ginseng into powder, sieving, and carrying out microwave-assisted extraction to obtain an extracting solution; then carrying out solid-phase extraction and purification on the extracting solution to obtain a purified solution; carrying out fluorescence derivatization on the purified liquid; and finally, carrying out HPLC-FLD analysis. According to the method disclosed by the invention, a microwave-assisted extraction-solid-phase extraction and purification combined fluorescence derivatization HPLC (High Performance Liquid Chromatography) technology is adopted, so that the defects of lengthy pretreatment and insufficient sensitivity in the traditional technology are overcome, and the dependence on scarce standard substances is also reduced.

The application discloses a kind of uranium nuclide automatic extraction device and method, wherein, device includes dissolving target device, acid regulator, extractor, purifier, waste liquid tank, product tank, control system and shielding box, dissolving target device, acid regulator, extractor, purifier, waste liquid tank, product tank are connected by conveying pipe and valve, liquid conveying power is provided by peristaltic pump, control system is set in shielding box, and relevant components in shielding box are electrically connected.The application also describes a method for extracting a uranium nuclide solution substantially free of impurities from an irradiated thorium target material.The uranium nuclide automatic extraction device of the present application can achieve rapid and efficient extraction and purification of uranium nuclides from irradiated thorium target material.The device has the characteristics of simple structure, compactness and automation operation, which greatly improves the production efficiency and reduces the radiation dose received by the operator.

The wastewater treatment system according to an embodiment of the present invention is used for treating wastewater containing a sodium sulfate solution, and comprises: an electrodialysis device for separating the sodium sulfate solution of the wastewater into a first solution containing sulfate ions, a second solution containing sodium ions, and circulating wastewater from which sodium sulfate has been removed; a first membrane distillation device that extracts purified water from the first solution in order to concentrate the first solution; and a second membrane distillation device that extracts purified water from the second solution in order to concentrate the second solution. According to the wastewater treatment system disclosed by the embodiment of the invention, salt compounds and other harmful ions in the wastewater can be selectively separated through the electrodialysis device so as to effectively separate a high-concentration ionic solution. Therefore, not only can substances after wastewater treatment be recycled to the maximum extent, but also the environmental harmfulness of salt compounds can be greatly reduced.

The invention relates to the technical field of medicine impurity purification, in particular to a separation and purification method of a high-purity urapidil impurity compound. Comprising the following steps: 1) performing vacuum concentration on urapidil mother liquor to obtain mother liquor concentrate M, adding dichloromethane, then adding 100-200-mesh silica gel, and performing vacuum concentration at 35-45 DEG C until the mother liquor concentrate M is dry; (2) weighing 200-300-mesh silica gel, adding dichloromethane, uniformly mixing, filling into a chromatographic column, and compacting for later use; (3) loading the vacuum concentrate in the step (1) into a chromatographic column, adding anhydrous sodium sulfate, and adding eluent for gradient elution; 4) collecting the eluent when the last eluent is used for elution, and performing reduced pressure concentration at 40-50 DEG C; after the concentration is finished, the urapidil impurity compound M1 can be obtained. According to the method for directly extracting and purifying the impurity compound from the mother liquor for preparing urapidil, the process is simpler, the purity of the purified impurity compound is 96-100%, and the accuracy and precision of the verification and detection method can be improved.

The invention provides a method for intensively extracting and purifying 5N-grade high-purity cobalt and nickel through ionic liquid, and belongs to the technical field of non-ferrous metal separation and purification. According to the method, the specific ionic liquid is selected as the extraction medium, matched with the synergistic extraction agent and combined with the micro-channel reactor to achieve efficient mass transfer, the Co / Ni and Mn / Cu separation coefficient can be remarkably increased, the ionic liquid recovery rate is increased, the 5N-grade high-purity cobalt-nickel product is finally obtained, and the process is easy for large-scale continuous production and has a wide application prospect. The defects of poor Mn and Cu impurity separation selectivity, high solvent loss rate and low process efficiency in the existing Co / Ni paragenic ore extraction and purification process are overcome. And meanwhile, the impurity separation selectivity is remarkably improved, the solvent loss is reduced, the process is efficient and environmentally friendly, the method is suitable for large-scale industrial production of high-purity cobalt and nickel, and key raw material guarantee is provided for the fields of new energy, electronic information and the like. The result of the embodiment shows that the cobalt and the nickel obtained by the invention both meet the 5N-level requirement.

The application relates to the technical field of preparation of shuganbin, and discloses a shuganbin rapid extraction and purification device and process.The device comprises a lower jar body, a filter disc, an assembling rod rotatably installed on the filter disc, an extrusion roller for extruding and dispersing raw materials rotatably installed on the assembling rod, and a transmission assembly; after the filter disc is lifted, the assembling rod is docked with the stirring shaft through the clamping assembly, the stirring shaft rotates to drive the assembling rod to rotate synchronously, the transmission assembly drives a plurality of dispersing wheels to rotate, the plurality of dispersing wheels can disperse the raw materials on the filter screen, then the extrusion roller rotates to the raw materials dispersed by rolling, the solution infiltrated in the shuganpi raw materials after filtration can be extruded, the solution most fully combined with the shuganpi raw materials can be fully extracted, the problem that the solution is wasted and the shuganbin cannot be fully extracted due to the direct removal of the solution after filtration is avoided, and the extraction efficiency of the shuganbin is greatly improved.

The invention discloses an extraction process of chaste tree twig oil in the technical field of plant essential oil extraction. An ethanol aqueous solution is adopted for extraction, ultrasonic treatment and a composite enzymolysis technology are combined, the chaste tree twig cell structure is effectively destroyed, and the grease extraction rate is remarkably increased. And then selectively adsorbing impurities in the extracting solution by using a specially-made cobalt ferrite-deep eutectic solvent-biochar magnetic composite material, and realizing rapid separation through an external magnetic field. And the alkalescent ethanol water solution is adopted for elution, so that the structure stability of the deep eutectic solvent is protected while impurities are effectively desorbed. And finally, carrying out rotary evaporation and column chromatography refining to obtain the high-quality vitex negundo oil. The process integrates various extraction and purification technologies, has the advantages of high extraction efficiency, mild operation conditions, small environmental pollution and the like, and is particularly suitable for efficient extraction and refining of the vitex negundo oil.

The invention discloses an effective part of a pulse-activating decoction as well as an extraction and purification method and application thereof, and the extraction and purification method comprises the following steps: taking red ginseng, radix ophiopogonis and schisandra chinensis as raw materials, adding water for extraction, and freeze-drying to obtain freeze-dried powder; dissolving the freeze-dried powder with distilled water, loading the dissolved powder on a macroporous adsorption resin column, carrying out gradient elution with water and a 20% ethanol aqueous solution in sequence, and separating fractions Fr1-Fr8; the chromatographic characteristics of each fraction are analyzed by using a C18 chromatographic column through a liquid chromatograph, the fractions with the same chromatographic characteristics are combined, the fractions Fr1-Fr4 are combined into a component yf-1, the fraction Fr5 is combined into a component yf-2, and the component yf-2 is the effective part of the pulse-activating decoction. According to the effective part disclosed by the invention, toxic parts are removed, so that H9c2 cell injury caused by adriamycin can be more effectively and stably protected with lower toxicity, and the application of the component yf-2 in preparation of medicines for treating myocardial cell injury is prompted.

The invention belongs to the field of analytical chemistry and food safety detection, and discloses a fish oil plasticizer liquid chromatography-tandem mass spectrometry detection method, which is characterized in that a target molecule is structurally modified through a specific chemical derivatization reagent, and the electrospray ionization efficiency is remarkably improved, so that a mass spectrometry response signal is enhanced; solid-phase extraction purification and high-performance reversed-phase chromatography separation are combined, so that effective removal of matrix interference and synchronous detection of various plasticizers are realized; the method does not need a complex solvent system, and is simple in operation flow and good in reproducibility; the detection limit reaches ppb level, the linear range is wide, the method is suitable for conventional and high-throughput detection of the plasticizer in fish oil and fish oil products, and a sensitive and reliable analysis tool is provided for quality safety assessment of related products.