415 results about "Extraction Purification" patented technology

The invention discloses a method for comprehensively recycling valuable metals from a spent lithium ion battery. The method comprises the following steps: carrying out electric discharge treatment on a spent battery, crushing, pre-roasting at 300-400 DEG C, adding a reducing agent, and carrying out reduction roasting at 450-700 DEG C; carrying out water extraction and evaporative crystallization on fine aggregates obtained through the reduction roasting, so as to obtain a high-purity lithium product, leaching copper, nickel and cobalt from leached slag and roasted lump materials by virtue of ammonia oxide, carrying out magnetic separation and sieving on ammonia leaching slag so as to obtain iron and aluminum enriched products, and carrying out reduction acid leaching, purification and edulcoration on sieved products, so as to obtain a high-purity manganese sulfate solution; and carrying out extraction and selective reverse extraction on ammonia leaching liquid, so as to obtain a high-purity nickel sulfate solution and a high-purity copper sulfate solution, and carrying out vulcanization cobalt precipitation, oxidation acid leaching and extraction purification on raffinate, so as to obtain a high-purity cobalt sulfate solution. The method is high in extraction rate of valuable metals and applicable to the treatment of multiple waste lithium ion battery raw materials and efficient utilization of multiple elements, and sorting is not required.

The combustion fume flowing in through line 1 is decarbonated by contacting with a solvent in column C2. The solvent laden with carbon dioxide is regenerated in zone R. The purified fume discharged through line 9 comprises part of the solvent. The method allows to extract the solvent contained in the purified fume. The purified fume is contacted in zone ZA with a non-aqueous ionic liquid of general formula Q⁺ A⁻; Q⁺ designates an ammonium, phosphonium and/or sulfonium cation, and A⁻ an anion likely to form a liquid salt. The solvent-depleted purified fume is discharged through line 17. The solvent-laden ionic liquid is regenerated by heating in evaporation device DE. The solvent separated from the ionic liquid in device DE is recycled.

The invention relates to a liquid-mass database for detecting residual drugs in animal-derived food and a use method thereof; preparation of the database includes the following steps: (1) preparing a standard work solution; (2) treating before analyzing, namely using an extraction purification technology combining fast enzymolysis and fast solid phase extraction (SPE) to treat to-be-detected samples before analyzing; (3) performing one-time sample introduction chromatographic analysis; and (4) building the liquid-mass spectral database.

The PAHs extraction purification determination method in the soil relates to 18 trace PAHs analysis and determination method in the soil. Using automatic Soxhlet extraction instrument to extract PAHs organic in the soil samples, and then using silica gel column chromatography to purify the extracted liquid, remove the polar and non-polar interference during extraction, and finally, using HPLC (with diode array detector) to analyze qualitatively and quantitatively 18 PAHs. In this invention, the extraction solvent consumption is small, and it can be used for large volume sample extraction, with quick sample analysis speed, low-cost sample pretreatment, and for the 5 group 13 isomers in 18 PAHs, it can not only accurately qualitatively but also accurately quantitatively determine, with low detection limit, high sensitivity, and with the exception of fluorine and perylene, the detection limit of the other 16 PAHs all below 10ng/g-dw. It is a rapid, sensitive and accurate analytical method for the trace PAHs, applied to farmland, wasteland, urban green belt and other soil.

A process for extracting high-purity solanesol from tobacco leaf includes mixing tobacco leaf with solvent, beating, filtering slurry, vacuum extracting of filtered cake in alcohol, filtering, collecting the filtrate, concentrating, saponfiying, extracting with n-hexane, washing with water, adsorbing, filtering, extracting the filtrate in alcohol, concentrating, freezing to educe out crystal, filtering, dissolving crystal in hot acetonitrile and low-temp educing of rystal twice. Its advantages are high purity (more than 95%), high output rate and low cost.

The invention discloses a method for extracting purified aescine from horse chestnut, which comprises the following steps: carrying out reflow degreasing on raw materials by adopting diethyl ether, then putting the obtained dregs of a decoction in an extractor; adding ethanol into the extractor to carry out ultrasonic extraction; after the ultrasonic extraction is completed, carrying out centrifugal separation on the obtained product so as to obtain aescine extracting solution; carrying out decoloration on the aescine extracting solution by active carbons; carrying out microfiltration and ultrafiltration on the aescine extracting solution subjected to decoloration; concentrating the obtained filter liquor until the concentration of the filter liquor is lower than that of ethanol; carrying out adsorption on the obtained filte with water and alcohol; collecting aescine fractions and recycling reagent; and standing the aescine fractions for crystallization and carrying out recrystallization on the obtained product by acetone and aether. The method is suitable for scale-up production.

The invention discloses a preparation method of high-purity phycocyanin, and is characterized in that the preparation method comprises the following steps: (1) taking a fresh spirulina powder, fully mixing with a phosphate buffer solution evenly, repeatedly freezing and thawing for 7-10 times to break and remove cell walls, centrifuging to remove spirulina mud, and thus obtaining a supernatant; (2) adopting a two-step precipitation method with a 20%-30% ammonium sulfate and a 40%-60% ammonium sulfate to obtain a phycocyanin crude extract; (3) after dialyzing the crude extract, loading the sample onto a weak anion exchange column DEAE Sepharose FF, carrying out gradient elution after ion exchange, collecting an outflow component with A620/A280 of more than 3; and (4) dialyzing the collected sample, then loading the sample onto a strong anion exchange column SOURCE30Q, carrying out gradient elution after ion exchange, collecting an outflow component with A620/A280 of more than 4, again carrying out one-time ammonium sulfate precipitation concentration, and thus obtaining the high-purity phycocyanin having the purity of more than 4.5. The extraction purification method is simplified in process, wide in source of the raw material spirulina, simple in required equipment, and high in purity of the product, and has quite high application value.

The invention discloses an efficient extraction method for blueberry anthocyanin. According to the method, blueberry fruits or blueberry leaves are taken as raw materials for extracting the blueberry anthocyanin and the method comprises extraction and purification of a blueberry anthocyanin crude extract and specifically comprises the following steps of carrying out enzymolysis and alcohol extraction in turn to obtain the blueberry anthocyanin crude extract, then purifying the blueberry anthocyanin crude extract, degreasing by extraction and removing impurities with macroporous resin to obtain the refined blueberry anthocyanin. According to the method disclosed by the invention, solvent extraction and enzyme extraction are simultaneously combined, enzymolysis is firstly used for improving the juice yield of the blueberry anthocyanin and a solvent is further used for extracting the blueberry anthocyanin. By adopting the extraction method, the extraction time can be greatly shortened, the active ingredients can be extracted to the greatest extent, and the extraction rate can be significantly improved; furthermore, the operation is simple and easy to operate, the parameters of the production process are stable, the product quality is stable, and the extraction method is suitable for industrial production; and in addition, the highest content of an extracted and purified blueberry anthocyanin fine product can achieve 49.6%, and the application value is very high.

The invention discloses a method for preparing a nickel-cobalt-manganese ternary material precursor by using a nickel-cobalt slag material. The method comprises the following steps: step (1), carrying out acid leaching treatment on the nickel-cobalt slag material with the nickel-cobalt mole ratio of 3/1 to 8/1 at the pH of 1 to 5 and the temperature of 30 to 80 DEG C, and then carrying out solid-liquid separation to obtain an acid leaching slag material with the nickel-cobalt mole ratio of 1: (0.9 to 1.1); step (2), carrying out hydrogen peroxide reduction leaching, chemical subtraction and extraction purification on the acid leaching slag material to obtain a nickel-cobalt solution; and step (3), adding manganese sulfate into the nickel-cobalt solution, and carrying out coprecipitation to obtain the nickel-cobalt-manganese ternary material precursor. In the method, the nickel-cobalt slag material is leached under the synergism of pH and temperature, the acid leaching slag material with the mole ratio of close to 1: 1 is obtained, then the reduction leaching, subtraction, purification and coprecipitation are carried out, and then the nickel-cobalt-manganese ternary material precursor meeting the requirement is obtained in one step.

The invention discloses an extraction, purification and preparation method of high-purity salvianolic acid B. The method comprises the following steps: (1) smashing a salvia miltiorrhiza medical material into fine powder, and screening with a sieve of 20-60 meshes; (2) adding an ethanol solution, soaking at the temperature below 5 DEG C, and carrying out ultrasonic extraction, wherein the extraction process temperature is controlled below 25 DEG C; (3) centrifuging extract, filtering, recovering ethanol at reduced pressure until the extract has no alcohol odor, concentrating the extract and filtering; (4) clarifying the extract, enriching through macroporous resin, and purifying through chromatographic column chromatography so as to obtain salvianolic acid B fraction; and (5) carrying out vacuum drying or freeze drying so as to obtain extractive. In the new process disclosed by the invention, cost is low, only ethanol is used as an organic solvent, and more than 90% of salvianolic acid B can be obtained through separation and purification, thus the method disclosed by the invention is suitable for industrial production.

The invention relates to the technical field of non-ferrous metal smelting hydrometallurgy and discloses an extraction purification method of cobalt nickel hydroxide hydrochloric acid leaching solution. The extraction purification process of the cobalt nickel hydroxide hydrochloric acid leaching solution comprises five links of soda saponifying, nickel saponifying, extraction purifying, organic load nickel washing and reverse extracting. P507 is selected from chloride media to be used as an extraction agent, a megilp (or sulfonated kerosene) is used as a diluting agent, the extraction agent and the diluting agent are used as organic phase, and the flow ratio of the organic phase to water phase is controlled according to different combining capacities of various metal ion extraction agents so that impurity metal ions in the solution are combined with the organic phase to be separated from garget metal Ni ions, and metal ions of Co, Cu, Ca, Mg, Mn, Zn and Fe in the cobalt nickel hydroxide hydrochloric acid leaching solution are further removed so that the cobalt nickel hydroxide hydrochloric acid leaching solution is deeply purified. The extraction purification method provided by the invention is simple in process flow and high in impurity removal efficiency, and the quality of prepared nickel chloride solution meets production requirements of an electroplating grade nickel chloride product.

The invention discloses a simple nucleic acid simplifying method which comprises processes of cracking, adsorption promotion, washing and elution. The method is characterized by comprising preparation methods and use methods of a simple purifying column, cracking liquid, adsorption promoting liquid and washing liquid special for nucleic acid purification, wherein the simple purifying column is a 1.5 ml centrifuge tube with a hole on the bottom; a small mass of absorbent cotton is compacted on the tube bottom; 0.3-1.0 g of glass powder with diameter of 120-130 mum is added as a purifying column body; an uncovered 2.0 ml centrifuge tube is sleeved outside the column body to serve as a collection tube, therefore, the collection tube is easily manufactured by using a common EP (Eppendorf) tube, cotton and glass powder. With a wide application range, the method is directly applied to the extraction and purification of genome DNA, plasmid DNA and total RNA, as well as the recycling, centrifugation and purification of PCR (polymerase chain reaction) products; the operation steps are concise and easy to master, and the requirements on the experiment operation technique are not high; moreover, the method has the advantages of high purification purity, high integrity, short purification time and low experiment cost; and as toxic substances such as phenol and chloroform are not used in the purification process, the method is environmentally friendly and energy-saving.

The invention provides a process for abstracting protodioscin from fenugreek, comprising: (1) pretreatment of the medicinal materials; (2) ultrasonic extraction; (3) process of extract; (4) purification: purifying and separating the extract by the process of macroporous adsorption resin and silica gel column chromatography. The technical features of the invention comprise: firstly, applying the macroporous adsorption resin technology to the preparation of the abstraction and purification of protodioscin, preparing high-purity protodioscin with a conventional method. The method has a simple process, the production cost is low, and product purity is high.

The invention discloses a vacuum pulse type method of extracting and purifying ursolic acid of rosemary. The method comprises the following steps: (1) drying and crushing a raw material medical material rosemary; (2) carrying out vacuum pulse type reflux extraction on the material by taking 20-90% of ethanol as a solvent, and concentrating the extracting liquor for later use; and (3) carrying out ultrasonic analysis on the concentrated ursolic acid extracting liquor after being adsorbed by D101 macroporous resin, and then, concentrating, crystallizing twice and drying to obtain a 90-98% ursolic acid product. The method provided by the invention efficiently and greenly extracts ursolic acid in the whole technical process under a condition that no organic solvents except ethanol are used, and the finally obtained ursolic acid product has the activities of bacteriostatic action, tumor resistance, inflammation dimishing and the like and is wide in application prospect.

The invention provides a method for wet extraction of purified microalgae oil. The method comprises the following steps: (1) adding an ethanol solution into ooze of collected microalgae, quickly stirring the mixture uniformly, carrying out centrifugation, and collecting sediments to obtain dehydrated algae ooze; (2) adding non-toxic or slightly-toxic organic solvent of a water solution of the organic solvent into the dehydrated algae ooze obtained in the step (1), carrying out cell disruption on the algae ooze, and extracting coarse oil in the microalgae; (3) carrying out centrifugation, collecting the coarse oil obtained in the step (2), adding water and inorganic salt into the coarse oil, stirring the mixture uniformly to form a coarse oil-water solution, adding n-hexane into the coarse oil-water solution, carrying out standing layering or centrifugal layering after uniform mixing to form three layers which are the upper solution layer, the middle solid matter layer and the lower solution layer, and collecting the solution on the upper layer; (4) carrying out desolvation on the solution on the upper layer to obtain the microalgae oil. According to the method for wet extraction of the purified microalgae oil, the yield of esterified grease is high, operation is easy, and energy consumption is low.

A method for detecting zearalenone toxin in traditional Chinese medicines in different matrices, including extraction, purification and detection of Chinese medicine samples, said samples are extracted homogeneously at high speed with methanol-water (80:20, V/V), pH7. 0 Tween-PBS buffer solution dilution, glass fiber membrane filtration, immunoaffinity column purification. The determination method includes: high performance liquid chromatography-fluorescence detection method, high performance liquid chromatography-diode array detection method determination, spectrum and high performance liquid chromatography-mass spectrometry confirmation. Liquid chromatography conditions are: mobile phase ratio methanol: acetonitrile: water (8:46:46, V/V/V), fluorescence detector wavelength: excitation wavelength: 270nm, emission wavelength: 440nm; diode array detector wavelength: 236nm . Liquid quality conditions: Methanol-0.1% ammonium acetate water (75:25, V/V) as mobile phase, atmospheric pressure chemical ionization positive ion mode, drying gas flow rate 6.00L/min, drying gas temperature 340°C, fragmentation voltage 150V, Full scan mode scan, scan range 200-400m/z. The invention has the characteristics of accuracy, precision, reliability and the like.

The invention discloses a preparation method of macroporous cross-linked sodium alginate gel beads. According to the method, an aqueous solution of a mixture of sodium alginate and sodium sulfate is dropped into calcium chloride solution; while sodium alginate forms gel beads, sodium sulfate forms calcium sulfate deposition; sodium alginate gel beads are cleaned with ethanol, and are subject to a reaction with epichlorohydrin, such that cross-linked sodium alginate gel is formed; calcium sulfate within cross-linked sodium alginate gel is solved; the obtained material is cleaned, such that macroporous cross-linked sodium alginate gel beads are obtained. The macroporous cross-linked sodium alginate gel beads prepared by the method has good bovine serum albumin absorbance, and has good application prospect in the respects of biologically-active substance extraction purification.

The invention discloses a rinsing liquid for nucleic acid extraction purification. The rinsing liquid comprises a buffer reagent, polyethylene glycol and a salt. The rinsing liquid reduces cross contamination in sample nucleic acid extraction purification, is free of an air drying process in extraction, reduces nucleic acid extraction purification time and improves nucleic acid extraction purification efficiency. A preparation method of the rinsing liquid has simple processes and can be operated easily.

The present invention relates to the extraction and purification method of low molecular weight heparin for treating blood coagulation and thrombus. The coarse low molecular weight heparin material is dissolved in NaCl solution and chromatographically separated with molecular sieve gel layer to obtain the chromatographic separated liquid, and the chromatographic separated liquid is alcohol precipitated, dewatered and dried to obtain low molecular weight heparin segment. Before chromatography, the coarse product is irradiated with ultraviolet lamp or reacted with oxidant sodium hypochlorite or hydrobromic acid to eliminate harmful ¿CN-NO- radical, and treated with strong anionic exchange resin to eliminate methanol and other chemical impurity. The present invention has the low molecular weight heparin product reaching relevant international standard, and the method is simple and high in yield.

The invention discloses an extraction and purification method of saikosaponin. The saponin part mainly contains saikosaponin a and saikosaponin d. The extract can be prepared by using a percolation method or a high-pressure percolation method, an ethanol solution is used for extracting bupleurum medicinal material, macroporous resin is used for purifying in the purification process, the total weight of the saikosaponin a and saikosaponin d in the prepared saikosaponin extract accounts for 30-60% of the weight of the extract, and the weight of the total saponins accounts for 50-80% of the weight of the extract. The method disclosed by the invention is simple to operate, has the advantages of low production cost, energy saving and high extraction efficiency, and is suitable for industrialized production.

The present invention discloses a method for identifying open chromatin sites of plant genomes by using micrococcal nuclease. The method comprises the following steps: (1) cross-linking plant material; (2) extracting and purifying cell nucleus of a plant material; (3) subjecting the cell nucleus to enzyme digestion with MNase in different concentrations; (4) detecting MNase digestion effect by 1.5% agarose gel electrophoresis; (5) selecting appropriate MNase for enzyme digestion of nuclear DNA, performing 1.5% agarose gel electrophoresis separation again, recovering DNA fragments, constructinga Illumina sequencing library, and performing sequencing; and (6) performing bioinformatics analysis on the sequencing data, and identifying the whole genome MH loci. The whole method is simple in process and short in time consuming, takes about 2.5 days in one cycle, and is high in visible enzyme digestion effect and suitable for most plant species. The method can be used directly used for identifying the core regions of functional DNA elements.