85 results about "Arctiin" patented technology

The invention relates to a method of preparing antivirus and antitumor natural medicine arctigenin, and its characteristic: crushing the Arctium lappaL., using organic solvent to degrease, and obtaining a crude arctiin, then using snailase to make fermentation and enzymolysis the on the crude arctiin in an enzyme reaction system, and finally terminating the enzymolysis reaction by alcohol-containing water-soluble organic solvent and extracting enzymolysis products, then separating the enzymolysis products by using silica gel column chromatography and solvent recrystallization, purifying and obtaining the pure arctigenin; the yield of the method is increased by at last over 20 times as compared with that of separating natural-existing arctigenin in plants like Arctium lappaL.

The invention provides a preparation method of arctium fruit extract for decreasing blood sugar, wherein the extract comprises Arctiin, Matairesinol, Lappaol A, Lappaol C, Lappaol Fú¼LappaolHú¼Arctignan Eú¼beta-sitosterol and daucosterol.

Present Invention relates to a skin whitening cosmetic composition comprising arctiin, arctigenin or a mixture thereof as an active ingredient. The disclosed composition can control or inhibit the synthesis of melanin by acting on a melanin synthesis route of α-MSH secreted by UV, and thus show an excellent whitening effect compared to a conventional skin whitening cosmetic. Also, the composition has high formulation stability and low skin irritation.

The invention relates to a preparation method of arctiin, which is simple to operate and has the advantages of low pollution and low energy consumption. The method comprises the following steps: taking dry ripe fruits of burdock,, adding into a CO2 supercritical extractor, extracting by using ethanol as an entrainer to obtain an extract, recovering the solvent while depressurizing, carrying out multistage countercurrent extraction by using an ethyl acetate-water (10:2) mixed solvent, merging the extract liquids, recovering the solvent while depressurizing, concentrating the solvent, adding the solvent to macroporous adsorbent resin columns to be adsorbed, eluting with ethanol, collecting the eluent, recovering ethanol while depressurizing, concentrating, adding acetone to crystallize, separating the crystals, washing and drying to obtain the arctiin. The method for preparing arctiin has the advantage of high product purity, and can easily implement industrial scale-up production.

The invention provides a method for preparing arctigenin, which includes the steps: arctiin is used as a substrate, Beta-glucosidase enzyme liquid produced by the fermentation of aspergillus niger CGMCC No. 2594 is used as a catalyzer, the subsrate and the catalyzer are transformed in a shaker at the temperature of 20 DEG C to 50 DEG C and at the speed of 100r/min to 200r/min for 40h to 64h; and the transformed solution is separated and purified to obtain the arctigenin. The method has the advantages of: 1. mild reaction conditions and being environmental-friendly; 2. simple preparation process of a biocatalyst, namely the Beta-glucosidase enzyme liquid, needing no complex zymoprotein separation and purification as well as low cost; 3. simple operation and relatively high biological transformation efficiency; and 4. easy amplification of the biological transformation process and easy realization of large scale industrialized production. By utilizing the method for producing, the molar yield of the arctigenin can reach 94.7 percent and the purity of the product can reach 99.7 percent.

Disclosed is a method for preventing and/or soothing dry or sensitive skin against external stresses, comprising contacting the skin with a composition including an agent that decreases the release of granulocyte-macrophage colony-stimulating factor (GMCSF) from keratinocytes in the skin, wherein under conditions of external stress, dry or sensitive skin is soothed and/or prevented. Agents that decrease the release of GMCSF include silybin, piperine, tetrahydropiperine, nortrachelogenine, rhapontin, arctiin, artigenin and mixtures thereof. Also disclosed is a method of identifying an agent that decreases the level of GMCSF released from keratinocytes.

A process for preparing arctiin and arctigenin used to prepare meticines features that the liquid extract of arctium fruit passes through a polyamid column for separating and purifying.

A construction method of an HPLC specific chromatogram of a wind-heat common cold granule includes the following steps: (1) preparation of a reference substance solution: precisely weighing a proper amount of an arctiin reference substance, adding 30-70% methanol to produce an arctiin methanol solution with concentration of the arctiin being 60-80 [mu]g/ml; (2) preparation of a test sample solution: finely grinding the wind-heat common cold granule, and precisely weighing about 1-3 g of the granule; (3) setting of chromatographic condition: employing a ZORBAX SB-C18 chromatographic column, employing a mixed solution of acetonitrile-0.1% phosphoric acid solution with ratio being 5-45:95-55 as a mobile phase, setting flow velocity to be 0.5-1.5 ml/min, preparing a DAD detector, wherein detection wavelength is set as 205-285 nm, setting column temperature to be 25-45 DEG C and setting number of theoretical plates to be not less than 10000; and (4) measurement: precisely sucking 5-20 [mu]l of each of the reference substance solution and the test sample solution, injecting the solutions into the liquid chromatographic instrument, and performing HPLC detection to obtain the HPLC specific chromatogram of the wind-heat common cold granule. The method can effectively monitor the quality of the wind-heat common cold granule in different batches. The method has high precision and good repeatability, and is used for comprehensively monitoring the quality of the product.

The invention belongs to the field of natural organic chemistry, and particularly relates to a method for enriching and purifying arctiin in burdock by using macroporous adsorbent resin. By using macroporous adsorption technology to separate and purify arctiin, the invention has the characteristics of good adsorption selectivity for arctiin, high adsorption and desorption speed, high adsorption capacity, abundant material sources, low production cost, obvious separation effect and high extraction purity, can be used for extracting arctiin conveniently and quickly, can be used to obtain a semi-finished product of which the content of arctiin is higher than 35% and a final product of which the content of arctiin is higher than 90%, and can overcome the defects of low extraction yield and low extraction purity in the conventional method. The macroporous adsorbent resin, which has the advantages of stable physicochemical properties, large surface area, high exchange speed, high mechanical strength, high antipollution capability and high thermal stability, is used for selectively adsorbing arctiin from the solution in a physical adsorption mode, and thus, the invention has the advantages of high adsorption and desorption speed, and high adsorption capacity.

An extract of eucommia ulmoides and its preparation method and application belong to the technical field of traditional Chinese medicine. The Eucommia ulmoides extract contains olivine 9-glucoside, olivine 4'-glucoside and arctiin, and their mass percentages are respectively 3.10-3.70%, 1.40-1.70%, and 9.50-10.50%. It is prepared by pulverizing Eucommia ulmoides, extracting, concentrating, centrifuging, standing, and separating with macroporous adsorption resin. Has a significant anti-inflammatory effect.

The invention discloses a guinea fowl feeding healthcare powder which is prepared from the follow raw materials in parts by weight: 0.8-1.2 parts of polygonatum, 0.5-0.7 part of angelica, 0.2-0.4 part of astragalus, 0.2-0.4 of isatis root, 0.2-0.4 part of medicated leaven, 0.2-0.4 part of arctiin complanatus, 0.2-0.4 part of semen astragali complanati, 0.2-0.4 part of bupleurum, 0.2-0.4 part of cassia twig, 0.2-0.4 part of cimicifugae foetidae, 0.2-0.4 part of pueraria, 0.2-0.4 part of epimedium, 0.2-0.4 part of orange peel, 0.2-0.4 part of garlic powder, 1-2 parts of carrot, 1-2 parts of hawthorn, 1-2 parts of silkworm chrysalis, 1-2 parts of black soybean meal, 1-2 parts of sweet potato leaves and a proper amount of honey. The raw materials are made into an extract by adopting the conventional method, and then the extract is dried and ground into powder. The guinea fowl feeding healthcare powder can be fed to the guinea fowls to improve the disease resistance of the guinea fowls effectively. The manure of the guinea fowls fed with the guinea fowl feeding healthcare powder is not smelly, and the guinea fowl feeding healthcare powder is particularly beneficial to purifying the environment of the large-scale guinea fowl farm. If the guinea fowl feeding healthcare powder is fed to the young guinea fowls, the meat of the guinea fowls in the growing stage has a unique flavor, and even the flavor still remains on the killed guinea fowls.

The invention discloses an extraction, separation and purification method of arctiin. The method comprises the following steps: a) ethanol extraction, namely grinding arctium lappa, adding an ethanol solution which is preferably 5-10 times by weight, carrying out reflux extraction for three hours for two to three times and mixing the extracting solutions; b) activated carbon decoloration, namely adding activated carbon which accounts for 0.5% of the extracting solution by volume, carrying out reflux decoloration and carrying out filtration, thus obtaining a decoloration solution; c) concentration, namely recycling the decoloration solution at reduced pressure and enabling about 20% by volume of ethanol solution to be remained; d) impurity removal, namely adding a mixed solution of petroleum ether and ethyl acetate, stirring the solution and extracting the impurities and repeating the step four-six times; e) arctiin extraction, namely adding a dichloromethane to extract product and repeating the step five-eight times; f) concentration and crystallization, namely mixing dichloromethane extracting solutions containing products, concentrating the extracting solution, adding methanol to dissolve the concentrate and adding 20% by volume of isopropyl ether for crystallization, wherein the concentration of the ratio of ethanol to water is 70-95%; the ratio of petroleum ether to ethyl acetate is (1:1)-(1:3). The method has the beneficial effects that the method is convenient to operate, is low in production cost and easily achieves industrial production.

The invention discloses a method for preparing arctiin, which comprises the following steps of: crushing burdock and extracting the crushed burdock by using water or alcoholic solution; allowing the extract to directly enter an absorption column to perform adsorption and enrichment, eluting the product with the water and the alcoholic solution in turn, and performing concentration under reduced pressure to obtain arctiin fraction; and performing countercurrent chromatographic separation on the arctiin fraction by using a two-phase system formed by aqueous solution consisting of a single organic solvent and salt to obtain salt arctiin fraction, and performing adsorption to remove the salt by using macroporous adsorption resin or ion exchange resin. The method has the advantages of simple process, continuous operation, contribution to automation and recovery and recycling of the solvent, and suitability for industrial mass production.

The present invention discloses the application of arctiin as one kind of Chinese medicine extract in preparing medicine for treating rhinitis and nasosinusitis. The medicine for treating rhinitis and nasosinusitis contains arctiin not less than 5 wt% and may contains other Chinese medicine components. The medicine may be prepared into capsule, granule, bolus, tablet, decoction or other oral preparation form. The present invention has the functions of clearing away heat and toxic material, dispelling wind, relieving stasis, eliminating inflammation and drawing out pus, and can treat rhinitis and nasosinusitis in high curative effect and no toxic side effect.

The invention provides a processing extraction method of siraitia grosvenorii fruits. The extraction method comprises the steps that the siraitia grosvenorii fruits are quickly washed, cut into segments, dried, taken out, soaked for 0.5 h by adding water of which the weight is 10 times the siraitia grosvenorii fruits, decocted for 1 h and then filtered; the medicine dregs are decocted for 1 h by adding water of which the weight is 8 times the medicinal materials, and medicinal liquid is filtered; first-time decoction liquid and second-time decoction liquid are mixed, and the mixed liquid is concentrated until the relative density is 1.05 at 60 DEG C; ethyl alcohol is added into the concentrated liquid to enable the volume percent of the alcohol content to reach 75%, stirring, standing and filtering are conducted, the filtrate is concentrated until the relative density is 1.25 at 65 DEG C, ethyl alcohol is recycled, and concentrated liquid is obtained; refining is conducted through silica gel, the selected silica gel is contained in resin columns in advance, impurities in the concentrated liquid are removed through reverse phase silica gel columns after the concentrated liquid is diluted by purified water, and all effluent is collected; the effluent continues to be concentrated, the concentrated liquid is dried and granulated to obtain siraitia grosvenorii fruit granules, and high-content mogroside V is prepared and is compatible with arctiin and catechin to prepare the siraitia grosvenorii fruit composition.