300 results about "Ultraviolet detectors" patented technology

The invention provides a gallium oxide ultraviolet detector based on a difunctional self-assembly passivation layer and a preparation method thereof, the gallium oxide ultraviolet detector comprises a substrate layer, an oxidant layer and the difunctional self-assembly passivation layer which are arranged from bottom to top, and two sides of the difunctional self-assembly passivation layer are provided with metal electrodes in contact with a gallium oxide layer. Wherein the bifunctional self-assembly passivation layer comprises a first passivation layer and a second passivation layer which are arranged from bottom to top, the passivation material of the first passivation layer is a material for reducing surface defects of the gallium oxide layer, and the passivation material of the second passivation layer is a material for enhancing the ultraviolet photon absorption rate. Passivation materials with different functions are combined to form the dual-function self-assembly passivation layer, and the dual-function self-assembly passivation layer is adopted to perform passivation treatment on the gallium oxide ultraviolet detector, so that the performance of the gallium oxide ultraviolet detector is jointly improved from two aspects of reducing surface defects of gallium oxide and enhancing ultraviolet photon absorption.

The invention relates to the technical field of semiconductor photoelectric detectors, in particular to a gallium oxide-based laminated structure ultraviolet detector and a preparation method thereof. The detector comprises a gallium nitride single crystal substrate, a GaON thin film layer and a beta-Ga2O3 thin film layer which are tightly stacked from bottom to top, and an electron transport layer and a hole blocking layer which are symmetrically arranged on the surface of one side, away from the gallium nitride single crystal substrate, of the beta-Ga2O3 thin film layer, and metal electrodes are arranged on the electron transport layer and the hole blocking layer. The material of the electron transport layer is ZnO, and the material of the hole blocking layer is TiO2. The device has the advantages that the device structure is stable, the response time can reach microsecond magnitude, and the descending time is short; the adverse effect caused by the inherent continuous photoconductive effect of the gallium oxide material is effectively overcome, the internal defects are obviously reduced, and the ultraviolet photoconductive gain is increased while the inherent continuous photoconductive effect of the gallium oxide is inhibited.

The present invention provides a kind of detection method of thirteen kinds of related substance contents in vonoprazan fumarate, and high performance liquid chromatography is used for detection, and the chromatographic conditions are: the test solution and the reference solution are injected into the high performance liquid chromatograph, and the chromatographic conditions are: the chromatographic column adopts octadecyl bonded silica gel column; the ultraviolet detector is used, and the detection wavelength is 260nm; the injection volume: 10μl; the column temperature: 30℃‑40℃; the flow rate: 0.8ml / min‑1.0ml / min; the gradient elution method is used, and the mobile phase A: the solution of 0.02mol / L potassium dihydrogen phosphate solution adjusted to pH 8.0 with potassium hydroxide solution and isopropanol mixed with a volume ratio of 95:5; the mobile phase B: the mixed solution of acetonitrile and isopropanol with a volume ratio of 95:5. The method can be used to detect the contents of 13 kinds of related substances in vonoprazan fumarate, and high performance liquid chromatography is used for detection, and the method has high detection sensitivity, and the verification results show that the system applicability and specificity of this method meet the requirements, and the needs of drug production quality monitoring can be met.

The invention discloses a gallium oxide solar-blind ultraviolet detector based on selective area etching and a preparation method thereof, and belongs to the technical field of semiconductor photoelectric detection. Comprises from bottom to top: a substrate; the first epitaxial layer is located on the substrate, and the first epitaxial layer is a highly doped n-Ga2O3 layer; the second epitaxial layer is located on the first epitaxial layer, and the second epitaxial layer is a lightly doped n-Ga2O3 layer; the third epitaxial layer is located on the second epitaxial layer, the third epitaxial layer is a highly-doped p-NiO layer, and the third epitaxial layer is distributed at intervals through selective area etching; and the first ohmic contact layer is located on the area where the third epitaxial layer is distributed at intervals. The detector adopts a PN junction structure working in a reverse bias state, and compared with a Schottky structure, the detector has the advantages that the built-in electric field intensity and the leakage current level of a device are less affected by high-energy photon irradiation and working environment temperature change, and the anti-irradiation performance of the device and the working capability under an extreme temperature condition are effectively improved.

The invention relates to a screening method and an activity evaluation method of a natural antioxidant, and the screening method is characterized in that a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant reaction system is constructed, and the activity of the natural antioxidant is evaluated through a high performance liquid chromatography-ultraviolet detector-tandem mass spectrometry analysis technology. Screening of the natural antioxidant is realized; according to the activity evaluation method, a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant + TMB reaction system is constructed, and the activity evaluation of the natural antioxidant is realized through an oxidized TMB photo-thermal detection technology. According to the method disclosed by the invention, the used reaction system is high in applicability, high in accuracy and good in selectivity.

The invention relates to an epitaxial growth method of a gallium oxide material, a gallium oxide epitaxial structure and an ultraviolet detector. The epitaxial growth method of the gallium oxide material comprises the following steps: providing a substrate; forming an aluminum nitride layer on the substrate; epitaxially growing at least one composite interface layer on the aluminum nitride layer, wherein the composite interface layer comprises an aluminum oxide layer and an indium oxide layer which are stacked in sequence; and epitaxially growing a gallium oxide layer on the composite interface layer. Therefore, the dislocation density and thermal stress in the gallium oxide layer can be reduced, and the quality and performance of the gallium oxide epitaxial structure can be improved. When the prepared gallium oxide epitaxial structure is applied to a photoelectric detector, the performance and the reliability of the device can be remarkably improved.

The invention discloses a flexible self-powered solar-blind ultraviolet detector based on gallium oxide and a preparation method thereof. The flexible self-powered solar-blind ultraviolet detector is specially designed to be used for efficient and low-power-consumption ultraviolet light detection in a complex environment. The detector is characterized by the design of the asymmetric electrode and the aluminum oxide passivation layer. The device specifically comprises a first metal electrode and a second metal electrode which are located on a gallium oxide thin film and can respectively form Schottky contact and ohmic contact through different technological processes and a patterned passivation layer according to different materials and sizes of the first metal electrode and the second metal electrode, so that the photoelectric property is effectively improved. The first metal electrode realizes high-quality ohmic contact by increasing the contact area, selecting materials and adopting an annealing process. And the second metal electrode realizes Schottky contact and improves the contact barrier through material selection and introduction of a passivation layer. The asymmetric electrode design can enhance a built-in electric field and effectively realize separation of photon-generated carriers, so that self power supply is better realized.

The invention belongs to the technical field of textile detection, and particularly relates to a textile anti-ultraviolet intensity detection device which comprises a detection machine body. The detection machine body is fixedly connected with an anti-ultraviolet detector; side plates are fixedly connected to the upper surface of the detection machine body and located on the two sides of the lower portion of the anti-ultraviolet detector; the two first electric sliding blocks synchronously drive the bottom disc and the top disc to be separated and combined, a textile sample is clamped between the bottom disc and the top disc and conveyed to the output end of the anti-ultraviolet detector for anti-ultraviolet intensity detection, a traditional manual feeding or semi-automatic feeding mode is replaced, the effect of automatic feeding detection is achieved, and the production efficiency is improved. The feeding speed and the anti-ultraviolet intensity detection efficiency are improved, a vacuum pump is used for being matched with four suction cups to conduct vacuum adsorption on a single textile sample, the adsorbed textile sample is driven by a second electric sliding block to slide to a base plate, and the effect of feeding the textile sample is achieved.

The invention provides a gallium oxide solar-blind ultraviolet detector based on nickel oxide quantum dots and a preparation method thereof. The preparation method comprises the following steps: S1, cleaning and drying a substrate on which gallium oxide grows; s2, performing etching on the surface of the gallium oxide to form a beta-Ga2O3 channel; s3, depositing laminated metal on the substrate as an electrode, and annealing to alloy the laminated metal so as to form ohmic contact with the gallium oxide; and S4, spin-coating p-type NiO quantum dots between the electrodes, introducing the p-type NiO quantum dots and the n-type beta-Ga2O3 through quantum dot integration to form a pn heterojunction gallium oxide ultraviolet detector, and improving the light responsivity and the response speed of the gallium oxide solar blind ultraviolet detector at the same time.

The invention relates to the technical field of iodide content detection, in particular to a method for determining radiochemical purity and iodine content of sodium iodide capsules for diagnosis by HPLC (High Performance Liquid Chromatography), which comprises the following steps: setting chromatographic conditions, and connecting a radioactive flow detector and an ultraviolet detector in series; preparing a disodium hydrogen phosphate solution, a glucose solution, a vitamin C solution, a first control solution, a potassium iodate solution, a second control solution, a carrier solution, a first sample solution, a second sample solution, a negative control solution and a test solution; and determining the radiochemical purity and iodide content of the first sample solution and the second sample solution in three batches based on the chromatographic conditions and the determination standard. According to the method, test parameters such as capsule sample analysis time, a pretreatment mode and a sample size are optimized, the radiochemical purity of the capsule is accurately determined, and the iodide content is controlled, so that the problem that main components cannot be accurately quantified and potential radioactive impurities cannot be detected by a traditional diagnosis sodium iodide [131I] capsule determination method is solved.

The invention discloses a diamond / gallium oxide ultraviolet detector and a preparation method thereof. The diamond / gallium oxide ultraviolet detector sequentially comprises a single crystal diamond substrate, an intrinsic single crystal diamond epitaxial layer, an finger-type ohmic electrode, a gallium oxide layer and a finger-type Schottky electrode from bottom to top, the finger-type ohmic electrode comprises an ohmic electrode strip array and an ohmic electrode pad which are connected with each other; the finger-type Schottky electrode comprises a Schottky electrode strip array and a Schottky electrode pad which are connected with each other; and the ohmic electrode strip array and the Schottky electrode strip array form a different-plane interdigital structure. Defects formed by the diamond layer and the gallium oxide layer are depleted, capture or recombination of interface defects to photon-generated carriers is reduced, electric field distribution can be optimized, collection of the carriers is facilitated, and the performance of the device is improved.

The invention provides a method for simultaneously detecting the purity of an antibody conjugate and the coupling ratio of a drug antibody based on a size exclusion chromatography method and application of the method. The method comprises the following steps: detecting and analyzing the purity of an ADC (Analog to Digital Converter) sample to be detected and the coupling ratio of the drug antibody by adopting an SEC-UPLC (Single Emission Computation-Ultra Performance Liquid Chromatography) serially connected with an ultraviolet detector; collecting a signal of the sample under the antibody characteristic ultraviolet absorption wavelength and the small molecule characteristic ultraviolet absorption wavelength at the same time; calculating the DAR value and the monomer purity of the ADC molecule according to the chromatographic absorption peak area; a mobile phase adopted by the SEC-UPLC is a sodium chloride-phosphate buffer solution containing 0-10vol% of an organic solvent; the ADC sample to be detected is an ADC molecule coupled with Dxd type small molecules. The invention provides an analysis method which is widely applicable to determination of the DAR value and the monomer purity of the ADC molecule coupled with the Dxd small molecules. The method can simultaneously solve the problems that the DAR value detection and analysis flux of the Dxd small molecules is low and the applicability is limited.

The invention relates to a preparation method of TiO2 and TiO2 / ZnO heterojunction based on a hollow structure and application of the TiO2 and TiO2 / ZnO heterojunction in an ultraviolet detector, the hollow structure TiO2 is a TiO2 hollow sphere, a TiO2 hollow cube and a TiO2 hollow dodecahedron, the hollow heterostructure is composed of the hollow structure TiO2 and a ZnO nanorod, the ZnO nanorod grows on the surface of the TiO2 hollow structure, and the whole ZnO nanorod is hedgehog-shaped; the prepared hollow-structure TiO2 and TiO2 / ZnO heterojunction is coated on a conductive surface of FTO conductive glass to prepare a working electrode, the working electrode is connected with a counter electrode through a heat sealing film, electrolyte is injected into an internal cavity to obtain the ultraviolet detector with high detection performance, and the ultraviolet detector is suitable for the field of ultraviolet detection. The method adopted by the invention has the advantages of simplicity in operation, low cost and high universality, different hollow-structure TiO2 and hedgehog-shaped TiO2 / ZnO heterojunctions such as spherical, cubic and dodecahedron heterojunctions are synthesized, an ultraviolet detector with a self-energy-supply characteristic is further prepared, and the application of a TiO2 nano material in the field of photoelectric detection is expanded.

The present invention discloses a SiC / Ga2O3 heterojunction single-photon ultraviolet detector and a method for fabricating the same. The method comprises: preparing a device functional layer and etching to form a mesa structure with an angle; depositing a first passivation layer on the upper surface of the mesa structure; etching the first passivation layer and an n+-type Ga2O3 epitaxial layer to form a photosensitive window; depositing Ni / Au metal on the upper and lower surfaces of the device, performing ohmic contact self-alignment, and then performing ohmic contact transparency treatment to form an anode transparent ohmic contact within each photosensitive window and a cathode transparent ohmic contact on the lower surface of the device; forming an anode metal electrode on the upper surface of the anode transparent ohmic contact located on the sidewall of each photosensitive window; forming a second passivation layer on the upper surface of the anode transparent ohmic contact within each photosensitive window, and forming a cathode metal electrode on the lower surface of the cathode transparent ohmic contact. The present invention solves the problem of poor device performance caused by using a single SiC material to fabricate single-photon ultraviolet detectors.

The invention provides a method for detecting taurine in magnesium acetyltaurate, which comprises the following steps: (1) derivatization treatment: carrying out derivatization reaction on a derivatization reagent and magnesium acetyltaurate containing taurine under an alkaline condition to obtain a derivatization product; (2) detection: detecting the derivatization product in the step (1) by adopting a high performance liquid chromatography-ultraviolet detector; wherein the derivatization reagent is selected from the group consisting of 2, 4-dinitrofluorobenzyl cyanide. The determination method has the advantages of high sensitivity, good specificity, simple operation, high accuracy, good reproducibility, good stability and low cost, improves the quality standard of taurine detection in magnesium acetyltaurate, can strictly control the quality of taurine in magnesium acetyltaurate, ensures the safety and reliability of magnesium acetyltaurate, and has a wide application prospect. The practical significance is realized.

The application discloses a method for detecting low-concentration urea in reclaimed water and a continuous online monitoring device, and belongs to the technical field of water quality monitoring equipment. The method comprises the following steps: adding sulfuric acid solution into a water sample to be detected to adjust pH to 3-5, then adding sodium dodecyl sulfonate and ethylenediaminetetraacetic acid to form a urea stable system; concentrating the water sample by vacuum distillation under the condition of 60-80 DEG C; adding a chromogenic reagent and a stabilizing reagent into the concentrated water sample, and reacting under the condition of 65-75 DEG C for 10-15 minutes; and determining the absorbance by using an ultraviolet detector and calculating the urea content. The application further provides a continuous online monitoring device for realizing the method. The application can accurately detect low-concentration urea below 80 mu g / L, the single detection time is less than 30 minutes, the anti-interference capability is strong, and full-process automatic control is realized.

The invention provides an optical fiber image transmission element for ultraviolet detection and a preparation method and application thereof, and the preparation method comprises the steps: carrying out the pickling of the cross section of one end of the optical fiber image transmission element, enabling the cross section to form a microporous structure with the depth of 10-50 [mu] m, and obtaining a pickled optical fiber image transmission element; a fiber core of the optical fiber image transmission element is made of an easily acid-etching material, and a cladding is made of an acid-etching-resistant material; the preparation method comprises the following steps: dissolving CsX and PbX2 in an organic solvent to obtain a precursor solution; wherein X is at least one of Cl, Br and I; the precursor solution is heated, gas is introduced, the gaseous precursor solution is brought to the microporous structure, the precursor solution reacts at the microporous structure in a gaseous form to generate CsPbX3 quantum dots, the microporous structure is filled, and a filled optical fiber image transmitting element is obtained; and the protection window is arranged on the filling surface of the filling optical fiber image transmission element, so that the optical fiber image transmission element for ultraviolet detection is obtained, the optical fiber image transmission element has the advantages of high brightness and high resolution, and the efficiency and the resolution of an ultraviolet detector can be effectively improved.

The invention discloses a method for detecting related substances of a fumaric acid Vonoprasone intermediate product by applying a high performance liquid chromatography, and the chromatographic conditions are as follows: a chromatographic column filler uses high-purity porous spherical silica gel particles coated with charged nano polymer microspheres, an ammonium acetate-acetonitrile system is used as a mobile phase, an ultraviolet detector is used as a detector, and the chromatographic column filler is used for detecting the related substances of the fumaric acid Vonoprasone intermediate product. According to the method, the detection of intermediate products 1 related substances, namely 4-dimethylaminopyridine and pyridine-3-sulfonic acid, generated in the production process of the Vonorazan fumarate is accurately realized, and a simple and reliable method is provided for the detection of the intermediate products generated in the production process of the Vonorazan fumarate.

The application discloses a thermoelectric pile structure for calibrating a far ultraviolet ultraviolet detector based on MEMS, which comprises a silicon substrate, a support layer, an absorption layer, a thermocouple pair, aluminum wires, aluminum electrodes and a single crystal silicon material structure on the silicon substrate. The double-layer thermocouple stacking structure is adopted to improve the device duty ratio, more thermocouple pairs can be laid in the case of keeping the size of the one-layer structure unchanged, and the response rate of the detector is improved. The circular structure conforms to the distribution trend that the temperature is radially diffused from the center of the absorption layer to the periphery in a decreasing manner, the hot end of the thermocouple pair can be closer to the area with higher temperature, the temperature of the hot end of the thermocouple pair is more uniform, and the response rate of the detector is improved. The radiant energy of the far ultraviolet ultraviolet radiation source is converted into an output thermoelectric electromotive force, a mathematical model of Fourier's law and the Seebeck effect is established for calculation, and the heat flux density of the far ultraviolet ultraviolet radiation source is measured.

The invention provides a gallium nitride heterojunction ultraviolet detector and a preparation method thereof. The gallium nitride heterojunction ultraviolet detector comprises a first electrode, a substrate, a two-dimensional h-BN layer, a non-doped GaN nucleating layer, a p-type GaN layer and a second electrode which are sequentially stacked. The two-dimensional h-BN layer is inserted to serve as an epitaxial template, so that the preparation process is simplified, the cost is reduced, and high-quality GaN film growth is realized. The two-dimensional h-BN is used as the tunneling layer, the gallium nitride heterojunction photoelectric detector structure is optimized, the atomic-scale thickness and the wide forbidden band energy band structure are utilized, carriers between heterojunctions can efficiently penetrate through the insulating layer through the quantum tunneling effect, an efficient vertical transport path is provided for photo-generated electron hole pairs, and the photoelectric detector efficiency is improved. Meanwhile, the interface passivation effect can effectively inhibit interface scattering and interface recombination, and extremely low dark current is achieved.

The application belongs to the technical field of radar and ultraviolet cooperative detection, and discloses a radar and ultraviolet cooperative detection system for extremely low RCS targets, which is composed of one radar, multiple ultraviolet detectors and a core processor, wherein the radar emits electromagnetic wave signals to space in an active manner, the ultraviolet detectors monitor ultraviolet radiation of targets in a passive manner, and the core processor processes the detected data and realizes cooperative detection of the radar and the ultraviolet system.The application effectively combines the advantages of traditional radar and ultraviolet detection, can make the detection system work effectively in various scenes, combines various target characteristic information detected by the radar and the ultraviolet detector to realize feature fusion judgment, and realizes effective detection of extremely low RCS targets; only when the extremely low RCS target is detected, the corresponding ultraviolet detector is started, so that fast identification and accurate detection of the extremely low RCS target can be realized; in a multi-target combat scene, the extremely low RCS target is effectively identified, so that the combat target is more reasonably planned, and the threat brought by the extremely low RCS target is greatly reduced.

The invention discloses a method for purifying Z-phenylalanyl tryptophan. The method mainly solves the technical problem that related by-products generated in the production process are difficult to remove. The purification method comprises the following steps: 1, preparing a reagent for preparation; 2, pre-treating a sample; 3, high performance liquid chromatography determination is carried out, a chromatographic instrument is an Agilent 1260 chromatographic instrument, a VWD ultraviolet detector is adopted, a chromatographic column adopts Waters Sun Fire C18, and a mobile phase is a solution A (A) with the molar concentration of 0.05% and an acetonitrile solution (B) with the mass percentage concentration of 100%; the method provides a reliable and convenient means for improving the purification purity of the Z-phenylalanyl tryptophan.

The application discloses a self-correlation instrument based on gallium oxide two-photon absorption ultraviolet femtosecond laser pulse width measurement, and belongs to the technical field of photoelectric regulation and control. The self-correlation instrument comprises a laser generating device, an optical delay system and a self-correlation signal acquisition device. The laser generating device is used for generating a first light beam and a second light beam. The optical delay system is connected with the laser generating device and is used for changing the time when the first light beam and the second light beam reach a sample to be measured. The self-correlation signal acquisition device is used for receiving two-photon photoelectric conversion signal change data caused by the sample to be measured being irradiated by the first light beam and the second light beam in sequence, and obtaining a self-correlation signal based on the two-photon photoelectric conversion signal change data. The application makes full use of the ultraviolet wave band absorption characteristics of gallium oxide to prepare an ultraviolet detector, and uses the self-correlation method to measure the photoelectric conversion signal generated by two-photon absorption of the gallium oxide ultraviolet detector to measure the pulse width of the 350-450 nm near-ultraviolet wave band femtosecond laser.

The invention discloses a gallium oxide film double-array device group for flame detection and identification and a preparation method and application thereof, and belongs to the technical field of semiconductor photoelectric devices and flame monitoring. The device group comprises two arrays which are complementary in function and independent in structure: a Ga2O3 ultraviolet detector array which is grown based on a high-temperature annealing sapphire substrate and is consistent in orientation and is used for performing high-signal-to-noise-ratio rapid detection on flame solar-blind ultraviolet radiation; and the non-oriented Ga2O3 photoelectric synapse array is deposited on the unannealed substrate, and learning and classification identification of flame spatio-temporal characteristics are realized by utilizing plasticity caused by defect capture of the non-oriented Ga2O3 photoelectric synapse array. In the preparation process, the orientation-controlled Ga2O3 active layer and the non-orientation Ga2O3 active layer are respectively obtained by controlling whether the sapphire substrate is subjected to high-temperature annealing treatment or not. The two arrays can form a solution integrating detection and identification. The scheme is simple in structure, low in power consumption, easy to expand and suitable for industrial security and protection, combustion monitoring, forest fire early warning and other scenes.

The application discloses a flexible self-powered day-blind ultraviolet detector based on gallium oxide and a preparation method thereof, which is specially designed for efficient and low-power-consumption ultraviolet light detection in a complex environment. The detector is characterized by asymmetric electrodes and an aluminum oxide passivation layer. Specifically, the first metal electrode and the second metal electrode are located on the gallium oxide film, and the two electrodes can form Schottky and ohmic contacts respectively through different processes and patterned passivation layers by virtue of the difference in material and size, thereby effectively improving photoelectric performance. The first metal electrode realizes high-quality ohmic contact through increasing the contact area, material selection and annealing process. The second metal electrode realizes Schottky contact and improves the contact barrier through material selection and introduction of the passivation layer. The asymmetric electrode design can enhance the built-in electric field and effectively realize the separation of photo-generated carriers, thereby better realizing self-power supply.

The invention discloses a synthesis and chiral detection method of N2-[fluorenylmethoxycarbonyl]-L-lysine allyl ester monohydrochloride, and mainly solves the technical problems of complex conventional chiral detection and difficult isomer separation. The synthesis and chiral detection method comprises the following steps: 1, preparing a reagent for preparation; 2, synthesizing a sample; 3, high performance liquid chromatography determination is carried out, an Agilent 1260 chromatographic instrument is adopted, a DAD ultraviolet detector is adopted, CHIRALPAKR AD-H is adopted as a chromatographic column, and a normal hexane solution (A) with the mass percentage concentration being 100% and an acetic acid-ethanol solution (B) with the mass percentage concentration being 0.1% are adopted as mobile phases; the invention provides a reliable and convenient means for improving the N < 2 >-[fluorenylmethoxycarbonyl]-L-lysine allyl ester monohydrochloride chiral detection method.

The embodiment of the invention provides a solar blind ultraviolet detector and a preparation method thereof. The solar blind ultraviolet detector comprises: a SiO2 / Si substrate; the electrode layer is arranged on the SiO2 / Si substrate; the absorption layer is arranged on the electrode layer, and the material of the absorption layer is lithium niobate; and the transparent conductive layer is arranged on the absorption layer, and voltage bias is applied between the transparent conductive layer and the electrode layer. The solar blind ultraviolet detector has the advantages of low dark current, easiness in large-scale manufacturing and the like.

The invention belongs to the technical field of analytical chemistry, and discloses a method for detecting creatinine in a creatine product, which comprises the following steps: (1) precisely weighing a sample, adding a buffer solution, carrying out vortex oscillation dissolution, volume metering, shaking up, centrifuging, and passing through a water phase filter membrane to obtain a creatine test solution; (2) a creatine test solution is taken, a liquid chromatograph is adopted for liquid phase detection, and the liquid chromatography conditions are as follows: an SCX chromatographic column is adopted, an ammonium dihydrogen phosphate solution is taken as a mobile phase for isocratic elution, an ultraviolet detector is adopted, and the content of creatinine is obtained according to a liquid phase detection result. According to the method, the situation that the detection result of the creatinine in the creatine product is virtually high can be remarkably reduced, accurate quantification of the creatinine byproduct creatinine is achieved, and a more perfect data basis is provided for quality control of the creatine product.

The invention discloses a method for detecting L-pyroglutamic acid and methionine sulfoxide in a compound amino acid injection, and relates to the technical field of medicine detection.Chromatographic conditions of a high performance liquid chromatography are as follows: gradient elution is performed by adopting a chromatographic column of an octadecylsilane chemically bonded silica filler and a mobile phase consisting of a mobile phase A and a mobile phase B, a detector is an ultraviolet detector, the detection wavelength is 205-210 nm, the flow velocity of a mobile phase is 0.7-1.2 ml / min, the sample size is 5-25 [mu] l, the column temperature is 20-25 DEG C, the mobile phase A is a mixed solution of an ion pair reagent, a phosphate buffer solution, methanol and acetonitrile, and the mobile phase B is one or more of a methanol solution, an acetonitrile solution and a phosphate buffer solution; the detection process comprises the following steps: injecting a test solution into a liquid chromatograph, recording a chromatogram, and calculating the contents of methionine sulfoxide and L-pyroglutamic acid by peak areas according to an external standard method.

The utility model relates to the technical field of protein purification, and particularly discloses a portable low-temperature protein purification box which comprises a box body, a control panel is mounted at the top of the box body, a temperature sensor is mounted on the inner wall of the box body, and a sample loading module, a purification module, a detection module and a sample collection module are arranged in the box body. The sample loading module comprises a sample liquid storage device, a buffer liquid storage device and a peristaltic pump which are mounted in the box body, the purification module comprises a chromatographic column connected with the peristaltic pump, and the detection module comprises an ultraviolet detector mounted in the box body. Through the design of the low-temperature control system and the heat preservation layer, protein purification can be carried out in a low-temperature environment, the activity of the protein is effectively maintained, and the problem that the protein is inactivated or even degraded due to the fact that the temperature is too high in a traditional purification method is solved; meanwhile, the influence of long-time low-temperature experiments on the body health of experiment operators is avoided.