97 results about "Pyrimethamine / Sulfadiazine" patented technology

A detection method simultaneously detects residue of multiple synthetic antibacterial agents in aquatic products by a high performance liquid chromatography. The synthetic antibacterial agents mainly comprise sulfadiazine SD, sulfamerazine SMR, sulfadimidine SDD, sulfadimethoxine SDM, furazolidone FZD, oxolinic acid OXA, nalidixic acid NAA and the like. The multiple synthetic antibacterial agents in an aquatic product sample are extracted by acetonitrile, degreased by normal hexane, concentrated, purified by a solid phase extraction column (alumina neutral) or a C18 solid phase extraction column, analyzed by a liquid chromatograph, detected by an ultraviolet detector, and quantified by an external standard method. The method can quickly, sensitively and accurately detect multiple target compounds to greatly reduce detection cost and shorten detection time.

The invention discloses a mixed bacterial agent capable of degrading antibiotics in soil, belonging to the field of microbial technology. The mixed bacterial agent is prepared by mixing Bacillus subtilis J5P2 and Pseudomonas J2 according to a volume ratio of (0.1-3): 1. A preparation method for the mixed bacterial agent comprises the following steps: I, preparation of a suspension; II, colony culture: (1) preparation of a medium and (2) culture; III, separation of strains; IV, subculture and domestication: (1) domestication, (2) preparation of a LB medium, and (3) preservation; V, preparation of inoculum liquid; and VI, preparation of the mixed bacterial agent. The mixed bacterial agent provided by the invention has the characteristics of capacity of effectively degrading a plurality of residual antibiotics in soil and reducing environmental pollution, etc., and can be used for the remediation of land contaminated by antibiotics such as tetracycline, penicillin, sulfadiazines and quinolones.

The invention provides a preparation method of a nano carrier for tumor photo-dynamics therapy (PDT). According to the preparation method, Fe<3+> salts are dissolved in DMF, then a photo-sensitizer (TCPP) is added to obtain particles (NMOFs); then the particles are dispersed in water under the assistance of ultrasonic waves; crosslinking agents (EDC and NHS) are added; after the reactions betweenBSA and sulfadiazine (SDs) finish completely, dialysis is performed to obtain particles (NMOFs@BSA/SDs); then the particles are dispersed in distilled water, then Mn<2+> salts are added, the pH is adjusted, and finally dialysis is performed to obtain a carrier (NMOFs@BSA/SDs@MnO2). Nano metal organic framework particles are taken as the basis and coated by protein (BSA) and sulfadiazine (SDs); finally the particles are in-situ mineralized to obtain required particles; SDs can specifically recognize carbonic anhydrase of tumors and is capable of actively targeting the oxygen-deficient parts oftumors; MnO2 generated in mineralization can catalyze the H2O2 decomposition to increase the oxygen content of tumors; and the PDT efficiency is improved.

The present invention relates to a method for determining 10 kinds of antibiotics in a water environment through combination of a sample pre-treatment technology and HPLC-MS, and belongs to the field of detection of safety of trace organic contaminant residue in the water environment. The method is characterized in that a water sample is separated and enriched through combination of solid phase extraction and dispersive liquid-liquid microextraction (SPE-DLLME), and then an ultra-high performance liquid chromatography-mass spectrometry instrument (UPLC-MS/MS) is adopted as a detection tool to directly determine the contents of 10 kinds of common antibiotics in the water environment (drinking water, tap water, river water, sewage treatment plant influent and effluent), wherein the 10 kinds of the common antibiotics respectively are sulfadiazine, sulfamethoxazole, oxytetracycline, tetracycline, doxycycline, ciprofloxacin, levofloxacin, chloramphenicol, cefuroxime axetil and tinidazole. According to the present invention, the water sample pre-treatment method and the instrument detection conditions are investigated and optimized, and the optimal SPE-DLLME-UPLC-MS/MS method is established and is successfully applied for the real sample determination; and compared with the traditional method, the method of the present invention has advantages of high sensitivity, high extraction recovery rate, wide application objects, environmental protection, and the like.

The invention provides a biotin-labeled sulfadiazine nucleotide aptamer. The biotin-labeled sulfadiazine nucleic acid aptamer is characterized by comprising a probe, wherein the nucleotide sequence of the probe is S-SDZ No.1. The kit and the method provided by the invention have the advantage of speediness, stability and simpleness in detection, and the prepared nucleotide aptamer is rapid in SDZ residue detection, high in sensitivity, good in repeatability and high in specificity, and has a wide application prospect in rapid detection of food safety.

Disclosed is a compound sulfonamide turbid agent which comprises the following constituents (by weight percent): sulfadiazine 8-12, sulfamethoxazole 4-8, sulfamethoxydiazine 2-6, trimethoprim 2-6, and medicinal auxiliary materials including acid-base scale modifier, suspension auxiliary agent, acidifying agent, anti-oxidant, stabilizer and purified water.

The invention relates to the technical field of environment detection and is suitable for measuring erythrocin, chloramphenicol, sulfamethoxazole, sulfadiazine, roxithromycin, cefotiam, pyridazol, norfloxacin, ofloxacin, tetracycline and doxycycline in sewage, surface water and bottom mud. After being sampled, a water sample and a mud sample are pretreated and detected through ultra-high performance liquid chromatography-triple quadrupole mass spectrometry, and qualitative diagnosis is conducted through the characteristic ion pair (m/z) of a target compound, a standard curve is drawn accordingto the response value and the corresponding concentration, and the external standard method is used in quantification, so that the contents of various antibiotics in the sample are obtained. The method is accurate, sensitive and simple, and is suitable for measuring the contents of 11 typical antibiotics in the sewage, the surface water and the mud at the same time.

The invention provides a sheath-core composite photocatalytic fiber and a method for preparing the same. The method includes steps of placing sheath-core composite polyester fibers in composite photocatalyst solution and carrying out padding treatment on the sheath-core composite polyester fibers; sequentially carrying out first heat treatment and second heat treatment on fibers obtained by means of padding treatment so as to obtain the sheath-core composite photocatalytic fiber. The sheath-core composite photocatalytic fiber and the method have the advantages that composite photocatalysts can be firmly attached onto the sheath-core composite polyester fibers by the aid of the padding treatment, polyester of core layers can have certain viscosity after the fibers are subjected to the first heat treatment and the second heat treatment, and accordingly excellent adhesion effects can be realized for the composite photocatalysts; as shown by experimental results, the sheath-core composite photocatalytic fiber is high in sulfadiazine degradation rate, and all sulfadiazine can be degraded by the sheath-core composite photocatalytic fiber in 5 hours approximately; the sulfadiazine degradation activity of the sheath-core composite photocatalytic fiber is hardly changed after the sheath-core composite photocatalytic fiber is reused by 20 times, and accordingly the sheath-core composite photocatalytic fiber is excellent in photocatalytic stability.

The invention discloses a preparation method and application of sulphonamide molecular-imprinting solid-phase extraction columella. The preparation method comprises the following steps of: (1) mixing a template molecule, a functional monomer and a cross-linking agent; (2) representing molecular-imprinting polymer particles; (3) filling the molecular-imprinting polymer particles into a solid-phase extraction columella, wetting and washing by using methanol and acetone, sealing in vacuum, wherein the sulphonamide molecular-imprinting polymer is obtained by the following steps of: (a) dissolving the template molecule sulfanilamide and the functional monomer in pore-foaming agent acetonitrile to obtain a mixed solution; (b) carrying out ultrasonic treatment onto the mixed solution by an ultrasonic cleaner to obtain a pre-polymerization system; (c) transferring the pre-polymerized system to a quartz reaction test tube, adding cross-linking agent and imitator; (d) placing the pre-polymerization system under ultraviolet light; (e) directly grinding the imprinted polymer to obtain the imprinted polymer particles. The preparation method of the sulphonamide molecular-imprinting solid-phase extraction columella is high in recovery rate, simple to prepare, low in cost and capable of being directly used for selective adsorption and efficient enrichment of sulfadiazine, sulfamerazine and sulfadimidine in animal-origin food.

The invention relates to a selective coloration culture medium for fast culturing and identifying clostridium perfringens. The selective coloration culture medium is characterized in that the preparation of 1000ml of the culture medium requires 12.0g-20.0g of casease hydrolysate, 6.0g-12.0g of yeast extract powder, 0.20g-0.80g of sodium sulfite, 0.005g-0.015g of polymyxin B, 0.05g-0.20g of sulfadiazine, 0.20g-0.80g of ferric citrate, 4-12 units of Y.S.N. and 10.0g-18.0g of agar powder and also requires the final addition of distilled water till the volume of 1000ml is achieved. The selective coloration culture medium has the advantages that the culturing time is obviously reduced when being compared with a conventional method and other bacteria with the same culturing characteristics are inhibited and do not grow so that the effect of fast clostridium perfringens culturing and identification can be realized.

The invention discloses a compound sulfadiazine injection and a preparation method thereof. The compound injection comprises the following components in parts by weight: 5-12 parts of sulfamonomethoxine sodium, 1-5 parts of trimethoprim (TMP), 0.1-0.3 part of anhydrous sodium sulfite, 0.01-0.05 part of disodium ethylene diamine tetraacetate, 8-10 parts of sulfamethoxazole, 20-50 parts of propylene glycol, 1-5 parts of ethanolamine and 45-79 parts of injection water. Compared with the single sulfamonomethoxine sodium, the compound preparation prepared from the sulfamonomethoxine sodium, the sulfamethoxazole and the TMP expands an antibacterial spectrum, enhances the curative effect of the injection and enlarges the clinical application range; and the compound preparation can be applied to treating infection caused by bacteria as well as fever, high fever and dyspnea caused by toxoplasma, and the preparation has better treatment effect on diseases such as appetite decrease and even anorexia, diarrhea and the like.

The invention provides an oral liquid preventing and curing gallinaceous leucocyto zoonosis and a preparation method thereof. The oral liquid comprises sulfadiazine, TMP, aluminum stearate, alcohol, sodium carboxymethyl cellulose, and Tween -80. The concrete preparation method comprises the following steps that: (1) the aluminum stearate is porphyrized, to which the alcohol is added, (2) the sulfadiazine and the TMP are added to the solution of the step (1), (3) distilled water is taken and stirred evenly, (4) the Tween -80 is added and stirred evenly, (5) the sodium carboxymethyl cellulose is taken and is mixed into paste with the distilled water and the paste is added to the solution of the step (4) to be mixed well, and (6) anhydrous sodium sulfate is added and dissolved, the distilled water is added and stirred evenly, the pH value is adjusted to 9.5 to 11.5 with 0.5 percent of sodium hydroxide water solution and the product is obtained. The oral liquid adopts the suspension technology. The slightly soluble sulfadiazine and TMP are made into suspension liquid. The procedure of stirring feed is left out. The use is convenient and the efficacy is obvious.

The invention relates to a method for degrading sulfadiazine in water by activating persulfate with coagulated sludge. The method comprises the following steps: 1, preparing a persulfate activator: carrying out natural air-drying, crushing, drying, crushing and sieving on the coagulated sludge; taking the crushed sludge, adding the crushed sludge into a tubular furnace, pyrolyzing the sludge in anitrogen atmosphere, and cooling the obtained pyrolysis product to obtain a black carbonized material; and 2, acting persulfate with the prepared carbonized material to treat sulfadiazine wastewater:adding carbonized material to the sulfadiazine wastewater, carrying out a reaction on a shaking table at room temperature, adding the persulfate, and continuing the reaction under the above conditions, wherein a detection result shows that the method has a high removal rate. The method has the advantages of realization of recycling of wastes by using the coagulated sludge as a main raw material, simple and easy preparation process, low cost, and great application prospect in the removal of antibiotics and other refractory organic pollutants.

The invention relates to a preparation method and application of a molecularly imprinted polymer of sulfadiazine for controlled catalytic degradation, belonging to the technical field of material preparation and pollution control of water environments. The molecularly imprinted polymer of sulfadiazine for controlled catalytic degradation is prepared according to the steps of preparation of a substrate material of a molecularly imprinted controlled catalytic material by loading catalytic inorganic nanoparticles on a surface of a silica nanosphere, and coating of a layer of imprinted polymer on the surface of the silica nanospher by a controlled polymerization technology. When observed through a transmission electron microscope, the catalytic material has a uniform microstructure and good dispersibility. The catalytic degradation capability of the catalyst is evaluated by a static catalytic degradation experiment. Experimental results show that the catalyst has certain selective catalytic degradation capability for sulfadiazine and good regeneration performance and can be used in certain potential applications.

The invention discloses a making method of macromolecular aquagel dressing of sulfanilamide pyrimidine salt, which comprises the following steps: dissolving N, N'-methylene diacrylamide into acrylic acid completely; dripping sodium hydroxide solution into acrylic acid; adding polyvinyl alcohol solution and chitose acetic acid solution; stirring evenly to obtain solution A; dispersing sulfanilamide pyrimidine salt powder into glycerin to obtain solution B; blending potassium persulfate solution and residual water completely to obtain solution C; dripping solution A in the solution B; stirring evenly; adding solution C; pouring into mould; heating to obtain the product to treat burn.

The invention discloses a sodium sulfadiazine compound preparation for treating pig brain hydropsy, and a preparation method and application thereof, and belongs to the technical field of veterinary medicines. The sodium sulfadiazine compound preparation comprises sodium sulfadiazine, mannitol, furosemide, trimethoprim and the like. The sodium sulfadiazine compound preparation is scientific and reasonable in formula, safe in auxiliary material, controllable in quality, high in stability and low in cost, and a preparation process is simple and is suitable for industrial production.

The embodiment of the invention discloses ointment for treating bedsore, diabetic wound and scald. The ointment comprises the following components in percentage by weight: 1.5 to 6.5 percent of sulfanilamide crystal, 1.5 to 6.5 percent of sulfadiazine, 2.0 to 10.0 percent of zinc oxide, 0.4 to 4.0 percent of cod liver oil, 0.1 to 3.0 percent of racanisodamine, 0.02 to 1.4 percent of borneol, 5.0 to 30.0 percent of herba violae, and the balance of medical vaseline. By adopting the ointment, tissue permeability at the afflicted part of a patient can be enhanced, moisture evaporation of the ointment is reduced, the acting time of the medicament at the afflicted part is prolonged, bacteria at the afflicted part are inhibited, local microcirculation is obviously improved, discontinuity during capillary blood perfusion is avoided, and healing of the afflicted part is promoted.

The invention discloses a method for simultaneous determination of nine sulfonamide antibiotics on suspended particles in water. The method comprises the following steps: taking a target water sample,filtering, and collecting a filtered membrane and particles retained on the surface; adding a quantitative internal standard substance; cutting and placing in a centrifuge tube after freeze drying; adding an appropriate amount of extractant; performing constant temperature oscillation, ultrasonic treatment and centrifuging; transferring supernatant to a K-D concentration bottle; purging the extraction liquid to less than 1ml by nitrogen gas; filtering with an organic microporous filter membrane; and measuring with an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer. The method has the advantages of short sample pretreatment time, simple steps, simultaneous determination of the contents of nine sulfonamide antibiotics such as sulfadiazine, sulfapyridine, sulfamethazine, sulfisomidine, sulfachlorpyridazine, sulfamerazine, sulfamethoxazole, sulfaquinoxaline and trimethoprim adsorbed on the suspended particles in water, and more accurate and true reflection of the contents of the sulfonamide antibiotics in water more.

The invention provides a hybridoma cell strain NaN-1 secreting a monoclonal antibody resistant to sulfonamide antibiotics (SAs) and an application thereof, belonging to the field of food safety immunodetection. The hybridoma cell strain NaN-1 secreting the common monoclonal antibody capable of identifying 25 SAs is collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.12028. The monoclonal antibody secreted by the cell strain NaN-1 can identify 25 SAs, has better detection sensitivity, enables the IC<50> of sulfadiazine (SD), sulfamerazine (SMR), sulfadimethoxine (SDM) and sulfathiazole (STZ) to be respectively 4.51mu g/L, 1.80mu g/L, 1.98mu g/L and 0.53mu g/L and can be used for multi-residue detection of SAs in food safety.

The invention discloses a composite sulfadiazine-trimethoprim nanoparticle preparation. The particle size of the nanoparticle preparation ranges from 1 to 100 nm. The nanoparticle preparation is prepared from sulfadiazine, trimethoprim, a macromolecule substance and a cosurfactant. The composite preparation is a nanoparticle preparation which is good in stability and high in solubility; the sulfadiazine and the trimethoprim in the preparation have remarkable synergetic effects, and with the combination of the two components, the antibacterial function of the preparation can be greatly improved, in addition, anti-medicine strains can be reduced; the composite nanoparticle preparation has the advantages of safety, high efficiency, wide spectrums, low drug resistance, and the like.

The invention discloses a compound sulfadiazine suspension. The compound sulfadiazine suspension comprises sulfadiazine, trimethoprim, sodium hydroxide, a suspending aid A, a suspending aid B, a surfactant and water for injection. The preparation method comprises steps as follows: sulfadiazine and trimethoprim are ground to 400-700 nm; trimethoprim is added to the suspending aid A; the mixture is dissolved, and a first dissolved solution is obtained; the water for injection is taken and heated to 45 DEG C, sulfadiazine and the suspending aid B are added, and a second dissolved solution is obtained; the rest water for injection is taken and heated to 30 DEG C-50 DEG C, sodium hydroxide is added and dissolved, the first dissolved solution is heated to 60 DEG C-65 DEG C, the second dissolved solution is heated to 40 DEG C-50 DEG C, then the first dissolved solution and the second dissolved solution are mixed, a third dissolved solution and the surfactant are added to a batching container and uniformly stirred, and a fourth dissolved solution is obtained; the prepared fourth dissolved solution is added to a high-speed homogenizer and sheared for 10-20 minutes, and filtrate is sterilized for 30 minutes at the temperature of 100-300 DEG C and under the pressure of 0.1-0.2 Mpa. The absorptivity is improved, the acetylation rate is decreased, and few crystals are formed in urine.

The invention discloses a synthetic method for sulfadiazine. The sulfadiazine is synthesized with sulphaguanidine and malonaldehyde as raw materials. The synthetic method has the advantages of being mild in reaction condition, short in reaction time, high in conversion rate and beneficial to industrial production.