423 results about "Diabetic wound" patented technology

The invention relates to self-healing double-layer hydrogel for promoting healing of diabetic wounds as well as a preparation method and application of the self-healing double-layer hydrogel, and belongs to the technical field of medical materials of surgical supplies containing macromolecular materials. The hydrogel comprises an inner layer and an outer layer, the inner layer is self-healing hydrogel in which microalgae cells are uniformly distributed, and the outer layer is self-healing hydrogel in which metal ion-humic acid coordination nanoparticle compounds are uniformly distributed, the self-healing hydrogel is formed by self-crosslinking of a macromolecular polymer containing an aldehyde group and a macromolecular polymer containing an amino group. The hydrogel provided by the invention has excellent biocompatibility, adhesion and self-healing performance, can meet the requirements of actual production and application, and has a relatively great application prospect as a dressing for chronic diabetes wounds; and the preparation process has the characteristics of simplicity in operation, low cost and mild reaction conditions, and is suitable for large-scale popularization and application.

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention belongs to the technical field of biomedical materials, and particularly relates to a hydrogel wound dressing and a preparation method and application thereof. The hydrogel wound dressing disclosed by the invention can be matched with a sound-sensitive agent by utilizing the temperature-sensitive shrinkage performance of the temperature-sensitive compound monomer, and shows ultrasonic thermal response shrinkage performance, specifically, the hydrogel wound dressing can be rapidly heated and remarkably shrunk under the ultrasonic action to promote wound shrinkage, so that wound healing is accelerated. The obvious potential is embodied in the aspects of promoting the repair of the wound infected by the drug-resistant bacteria and the repair of the wound infected by the drug-resistant bacteria and diabetes mellitus. When the prepared hydrogel wound dressing is used, the contraction degree of the hydrogel wound dressing can be controlled by adjusting the ultrasonic time in the treatment process, and protection and treatment effects on drug-resistant bacteria wounds are achieved in a programmed mode. Meanwhile, the fixing structure of the hydrogel can anchor added drugs such as a sound-sensitive agent and the like, and the action time of the drugs is prolonged.

The invention relates to nanoparticle-loaded antibacterial composite hydrogel as well as a preparation method and application thereof, a hydrogel network main body is formed by grafting phenylboronic acid to methacrylated I-type collagen rCol1MA-PBA and methacrylated polylysine EPLMA and adding glucose oxidase GoX for crosslinking, and is loaded with core-shell nano-enzyme C (at) Z (at) T at the same time, and the core-shell nano-enzyme C (at) Z (at) T is loaded with the core-shell nano-enzyme C (at) Z (at) T and the core-shell nano-enzyme C (at) Z (at) T; the core-shell nano-enzyme C (at) Z (at) T is composed of a spherical CeO2 nano-particle core, a zeolite imidazate framework material TIF-8 shell and tannic acid TA coating the shell. The raw materials of the hydrogel are easy to obtain, the preparation method is simple, the price is low, and the components are safe and reliable; in addition, multiple functions of resisting bacteria, resisting inflammation, resisting oxidation, promoting angiogenesis and the like are integrated, a more comprehensive treatment effect is provided, and the dressing can be used for healing the wound surface of diabetes mellitus.

The invention discloses a preparation method of a zinc-dihydromyricetin nano-enzyme and application of the zinc-dihydromyricetin nano-enzyme in preparation of drugs for promoting healing of diabetic wounds, and the zinc-dihydromyricetin metal polyphenol nano-enzyme (Zn-DHM) is obtained by coordinating a metal element zinc (Zn) and a natural product dihydromyricetin (DHM) through a one-step method by taking PVP as a surfactant. The Zn-DHM nano-enzyme prepared by the invention has antioxidant property and cell proliferation and migration promoting ability, and can maintain metabolism and immune homeostasis of diabetic wounds, so that the Zn-DHM nano-enzyme can accelerate early healing of the diabetic wounds.

The invention provides a fiber dressing for promoting wound healing and a preparation method and application thereof, and belongs to the technical field of biological materials. The shell of the fiber dressing contains the first medicine for promoting polarization of macrophages, and the first medicine can promote polarization of macrophages at normal and diabetic wound surfaces from M1 type to M2 type; a shell substrate is a mixture of hydrophilic and hydrophobic polymers, so that painless adhesion and stripping of a wound surface can be realized by absorbing seepage and spraying non-irritant normal saline on the wound surface, and secondary injury of the wound surface is reduced; an inner core contains a second medicine and a third medicine which are used for inducing death of M1 type macrophages, so that wound inflammatory response can be reduced, wound bacteria can be killed, wound infection degree can be reduced, and wound healing can be accelerated; the core matrix is a mixture of hydrophilic and hydrophobic polymers with good biocompatibility, can provide mechanical support for the fiber dressing, and provides a slow release effect for the two substances in the core. By controlling the composition of the inner core and the shell, the healing of normal wounds and diabetic wounds can be effectively promoted.

The invention relates to application of sea cucumber-derived nano-vesicles in preparation of a medicine for promoting wound repair. The nano-vesicles (SC-EVs) extracted and prepared from sea cucumbers can effectively promote cell migration, scratch repair and tube forming capabilities of human skin epithelial cells (HACAT) and human skin microvascular endothelial cells (HM DEC) and inhibit cell apoptosis, so that the capability of repairing cells is achieved, and particularly the effect of repairing wound surfaces in a high glucose state or diabetes mellitus is achieved. The invention provides a new direction and thought for the application of the sea cucumber-sourced nano vesicles (SC-EVs), provides a brand new drug research and development and clinical treatment direction for wound repair, especially for solving the problem of difficult healing of wounds of patients with high glucose or diabetes, and has extremely high social value and market application prospect.

The invention discloses multifunctional hydrogel for promoting healing of diabetic wounds and a preparation method of the multifunctional hydrogel. The multifunctional hydrogel is prepared from Ti3C2Tx-Ag, paeoniflorin (PF), a gel matrix oxidized konjac glucomannan (OKGM) solution and carboxymethyl chitosan (CMCS). The method comprises the following steps: mixing Ti3C2Tx powder with AgNO3 in deionized water, heating to boiling, adding a proper amount of a sodium citrate solution, reacting to obtain Ti3C2Tx-Ag (TA) powder, and mixing the powder with a paeoniflorin (PF) and oxidized konjac glucomannan (OKGM) solution and a carboxymethyl chitosan (CMCS) solution to prepare the KC-PF-TA hydrogel. The multifunctional hydrogel has excellent photo-thermal antibacterial performance, anti-inflammatory performance and angiogenesis promoting performance, shows a remarkable effect of promoting healing of diabetic wounds through the synergistic effect of Ti3C2Tx-Ag and paeoniflorin, and has good biocompatibility; the multifunctional nano-composite hydrogel disclosed by the invention has great application prospect and value in the field of wound repair materials.

The invention discloses riboflavin photodynamic antibacterial hydrogel for a diabetes wound dressing as well as preparation and application of the riboflavin photodynamic antibacterial hydrogel, and belongs to the technical field of biomedical materials. The hydrogel is prepared from carbomer 941, rhizoma bletillae polysaccharide and carboxymethyl chitosan through physical and chemical bi-crosslinking, glutaraldehyde and carboxymethyl chitosan are added to form dynamic imine bonds, and the stability and adaptability of the hydrogel are enhanced. Meanwhile, the photosensitizer riboflavin is introduced, so that the hydrogel is endowed with photodynamic sterilization characteristics, efficient antibiosis is realized, and rapid healing of diabetic wounds is promoted. The hydrogel has excellent wound environment adaptability and adhesion performance, and is suitable for complicated wound surfaces of diabetes mellitus. The successful research and development of the invention provide reference for the development of novel photodynamic wound dressings, have wide application prospects, provide better treatment choices for diabetics, and are helpful for accelerating wound healing, reducing infection risks and improving the life quality of patients.

The invention discloses a double-layer gel patch for diabetic wounds and a preparation method of the double-layer gel patch. The double-layer gel patch is composed of an upper gel layer and a lower gel layer, and when the double-layer gel patch is used, the lower gel layer is attached to the surface of skin; the upper gel layer is prepared from methacrylated gelatin, oxidized hyaluronic acid, a photoinitiator and an anti-inflammatory drug, and the lower gel layer is prepared from 3-carboxyphenylboronic acid grafted gelatin, dihydrocaffeic acid grafted epsilon-polylysine and an antibacterial agent. The double-layer gel disclosed by the invention has high mechanical strength and absorbability, and meanwhile, a sequential treatment strategy of firstly resisting bacteria and then resisting inflammation can be realized.

The invention discloses a photo-thermal composite hydrogel patch and a preparation method and application thereof.The hydrogel patch is of a three-dimensional latticed porous structure formed by taking methylacryloyl gelatin (GelMA) and methylacryloyl hyaluronic acid (HAMA) as matrixes, loading niobium carbide nano-particles (Nb2CNPs) and vinipofen (VP) and conducting 3D printing or casting molding, the hydrogel patch is named as VP-Nb2C-coated GelMA / HAMA (VNGH) hydrogel patch for short. The VNGH has good photo-thermal antibacterial property, oxidation resistance and reepithelization promoting function, can significantly improve the quality of mouse diabetic wound healing, and provides a new thought and strategy for realizing scar-free healing of diabetic wounds.

The invention discloses a double-response collagen-based conductive hydrogel, a medicine and a preparation and application method of the double-response collagen-based conductive hydrogel. The preparation method of the double-response collagen-based conductive hydrogel comprises the following steps: carrying out chemical modification on polysaccharide through 3-aminophenylboronic acid, and then carrying out sodium periodate oxidation treatment to obtain a dialdehyde polysaccharide grafted aminophenylboronic acid copolymer; the preparation method comprises the following steps: performing hydrazide modification on collagen through adipic acid dihydrazide, and performing free radical polymerization with aniline to obtain a hydrazide collagen grafted polyaniline copolymer; the dialdehyde polysaccharide grafted aminophenylboronic acid copolymer and the hydrazide collagen grafted polyaniline copolymer are subjected to crosslinking, and the double-response collagen-based conductive hydrogel capable of responding to glucose and the pH value is obtained. Compared with single-response conductive hydrogel, the hydrogel can specifically respond to the microenvironment of chronic diabetic wound surfaces and release antibacterial and anti-inflammatory drugs, and high-quality healing of chronic wounds such as diabetic foot ulcers and the like is promoted.

The invention discloses a conductive microneedle patch for diabetes wound healing and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The conductive microneedle patch is of a nesting doll type double-layer structure formed by an inner layer and an outer layer, microneedles on the inner layer are constructed through a methacrylic anhydride modified gelatin template pouring method, and polyvinyl alcohol microneedles on the top layer are formed by the outer layer through a lining-template pouring method. Wherein the microneedle patch has remarkable conductivity and antibacterial property due to a conductive nano-material silver nanowire in the inner-layer microneedle, and the outer-layer microneedle is loaded with a hypoglycemic drug and a natural humectant betaine, so that the properties of moisturizing and continuously releasing drugs are achieved. The obtained microneedle patch can keep a wound moist environment and adjust a diabetes wound inflammation microenvironment, cell and blood vessel growth is promoted through exogenous electrical stimulation, and rapid healing of a diabetes wound can be effectively achieved by releasing a hypoglycemic drug.

The invention discloses a multi-drug truncation microneedle, a preparation method and application of the multi-drug truncation microneedle in promoting diabetic wounds, and belongs to the technical field of drugs. Comprising a backing (1) and an array composed of a plurality of multi-drug cutting needles (2) on the backing, each multi-drug cutting needle is of a microneedle structure and comprises a gel slow-release needle tip (21) and a water-soluble instant needle body (22), the gel slow-release needle tip (21) and the water-soluble instant needle body (22) form a complete microneedle, and the water-soluble instant needle body (22) and the backing (1) are fixedly connected into a whole. The space-time delivery of the angiogenesis promoting medicine and the angiogenesis maturing promoting medicine is realized, and the angiogenesis and angiogenesis maturing processes in the chronic wound of diabetes mellitus can be accurately regulated and controlled.

The invention discloses a preparation method and application of photo-thermal antibacterial hydrogel for MRSA (Methicillin Resistant Staphylococcus Aureus) infected diabetes wounds, and relates to the technical field of biological tissue engineering. The method comprises the following steps: uniformly dispersing ZIF-8 (at) PDA nanoparticles in a carboxymethyl chitosan solution, adding a gelatin solution, uniformly mixing, adding into an oxidized sodium alginate solution, and carrying out a Schiff base reaction to obtain the photo-thermal antibacterial hydrogel for MRSA infected diabetes wounds. The hydrogel disclosed by the invention is prepared by crosslinking carboxymethyl chitosan, gelatin, oxidized sodium alginate and dopamine modified ZIF-8 nanoparticles through a Schiff base bond. Carboxymethyl chitosan (CMCS) has excellent biocompatibility, antibacterial activity and adjustable and controllable mechanical properties, and can effectively preserve moisture, form a breathable and moist environment and promote skin healing.

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-layer multifunctional hydrogel microsphere for diabetic wounds as well as a preparation method and application of the multi-layer multifunctional hydrogel microsphere. The preparation method comprises the following steps: firstly, synthesizing a chitosan derivative with proper amino and aldehyde group contents, oxidized macromolecules and modified chitin; then preparing photocuring hydrogel microspheres by utilizing a microfluidic technology, and sequentially immersing the photocuring hydrogel microspheres into an oxidized polymer solution, a modified chitin solution, a tannic acid solution and a metal salt solution, so as to construct the hydrogel microspheres with a multifunctional layer structure. The invention further provides specific application intervals of parameters such as solvents used by various solutions, concentration ranges and soaking time. The preparation method provided by the invention has expansibility and universality, various types of microspheres can be constructed for sequential release of bioactive substances, and the requirements of tissue repair are met.

The invention discloses a bletilla striata polysaccharide hydrogel dressing and a preparation method and application thereof.The preparation method of the bletilla striata polysaccharide hydrogel dressing comprises the following steps that a carboxymethyl chitosan solution, a bletilla striata polysaccharide solution and cinnamyl aldehyde are mixed and stirred to be uniform at the temperature of 20-30 DEG C, a mixed solution system is prepared, and then standing is conducted to form gel; wherein the volume ratio of the carboxymethyl chitosan solution to the bletilla striata polysaccharide solution is (1-5): 1, and the concentration of the cinnamyl aldehyde in the mixed solution system is 0.05-5 wt%. The preparation process is simple, the raw materials are all green and natural sources, and the prepared hydrogel is good in biocompatibility, has good antibacterial, anti-inflammatory and angiogenesis promoting effects and can accelerate diabetic wound repair.

The invention discloses a high-activity injectable self-healing temperature-sensitive hydrogel dressing and a preparation method and application thereof, the high-activity injectable self-healing temperature-sensitive hydrogel dressing is obtained by forming a temperature-sensitive hydrogel through a two-dimensional nano material MXene aqueous solution and F127 under the hydrogen-bond interaction, combining an M2 macrophage source exosome on MXene under the electrostatic adsorption action, and wrapping the MXene through a network structure of the hydrogel. The preparation method is simple, an obtained sample is free of organic solution residues, reaction conditions are mild, operation is convenient, the raw material cost is low, the hydrogel dressing has temperature-sensitive, injectable and self-healing performance, the hydrogel dressing has temperature-sensitive and injectable performance, is sol at normal temperature, forms gel after making contact with skin and is convenient to smear and use on wounds, and experimental results prove that the hydrogel dressing has good application prospects. The hydrogel has good biocompatibility and relatively strong antibacterial performance, and can effectively play anti-inflammatory and vascular regeneration promoting roles in diabetic wounds and promote rapid repair of the diabetic wounds.

The invention discloses Janus microspheres co-loaded with melanin and tea polyphenol-magnesium as well as a preparation method and application of the Janus microspheres. The preparation method comprises the following steps: complexing tea polyphenol and magnesium ions under an alkaline condition to form nano-particles, and dissolving keratin in hair by using strong base to obtain melanin nano-particles; the two solutions are respectively mixed with methacryloyl collagen to prepare a solution as a mobile phase, vegetable oil is used as a dispersion phase solution, in a microfluidic device, a two-channel flow phase is coaxial and co-flow, the dispersion phase fluid disperses the mobile phase into monodisperse liquid drops by using a flow shear force, and the monodisperse liquid drops are subjected to ultrasonic dispersion. And curing the liquid drops under the irradiation of ultraviolet laser to form the Janus microspheres co-loaded with melanin / tea polyphenol-magnesium. By means of the microfluidic technology, high-uniformity preparation of the Janus microspheres co-loaded with melanin / tea polyphenol-magnesium is achieved, synergistic anti-oxidation and antibacterial treatment can be achieved, and the Janus microspheres can be applied to various biomedical applications with anti-oxidation and antibacterial requirements, such as promotion of healing of diabetic wounds, bacterial infectious wounds and the like.

The invention relates to the technical field of biological medicine, and particularly provides visual hydrogel based on multiple response mechanisms as well as a preparation method and application of the visual hydrogel. The preparation method comprises the following steps: (1) preparing an inclusion compound from beta-cyclodextrin, hydroxypropyl-beta-cyclodextrin and anthocyanin; (2) respectively preparing a polyvinyl alcohol / rhein solution and a mixed solution of quaternary ammonium salt chitosan / hyaluronic acid / kappa-carrageenan; and (3) initiating dynamic crosslinking through phenylboronic acid, constructing a multi-network structure by cooperating with tannic acid, the clathrate compound and genipin, and performing gradient temperature control curing to form the hydrogel. The hydrogel has the pH / temperature / ROS response characteristic, the wound surface microenvironment state can be fed back in real time by means of anthocyanin color change, meanwhile, hyaluronic acid / polyvinyl alcohol can construct a 3D microchannel structure, ordered arrangement of collagen is promoted, wound surface healing is accelerated, and the wound surface healing effect is improved. Therefore, the problems that direct loading of anthocyanin is unstable, and a single response mechanism is difficult to adapt to a complicated microenvironment of a diabetic wound surface are solved.

The composition is of a network structure and is composed of strontium ions and chlorogenic acid, and the weight ratio of the strontium ions to the chlorogenic acid is 1: (9-11). The composition and hydrogel are mixed to prepare a microneedle patch, and the microneedle patch is prepared into a medical instrument for wound repair. Through verification, the SrC-MPNs is continuously released by the SrC-MPNs (at) MN-PP, so that the substances are dissolved and absorbed into tissue fluid around a wound, and strontium ions and chlorogenic acid in the SrC-MPN can stably and continuously exert the effects of resisting bacteria and oxidation, promoting angiogenesis, promoting wound healing and the like. An in-vivo experiment result shows that the SrC-MPNs (at) MN-PP can promote in-vivo and in-vitro wound healing, promote M2 macrophage polarization, remove excessive ROS and promote angiogenesis and tissue regeneration so as to promote healing of diabetic wounds.

The present invention relates to the technical field of diabetes, and in particular to a controlled-release diabetic wound healing promoting treatment method, comprising the following treatment experimental steps: step S1: preparation of Hst1@PNPs; step S2: Coomassie brilliant blue staining; step S3: scanning and transmission electron microscopy detection; in the present invention, a slow-release Hst1 treatment method is constructed by encapsulating Hst1 into platelet membrane vesicles derived from autologous blood. On the one hand, the PNPs encapsulate the linear Hst1 peptide structure to prevent it from being rapidly degraded by high proteases in diabetic wound exudate, thereby improving bioavailability. On the other hand, the PNPs can adsorb excess LPS in the wound exudate, and cooperate with Hst1 to achieve anti-inflammatory and anti-oxidative stress effects. Subsequent cell and animal experiments further confirmed that Hst1@PNPs have the effects of anti-inflammatory, scavenging ROS, promoting re-epithelialization, collagen deposition and angiogenesis, thereby promoting diabetic wound healing.

The invention provides an endometrial mesenchymal stem cell-derived exosome and application thereof in preparation of a medicine for treating diabetic wounds, and belongs to the technical field of biological medicines. The endometrial mesenchymal stem cell-derived exosome provided by the invention can effectively promote HUVEC tube forming ability, promote wound angiogenesis of diabetic model mice, increase collagen deposition and reduce wound area, finally achieves the purpose of promoting healing of diabetic wounds, and solves the problem of limited treatment effect of exosomes in the prior art. The endometrial mesenchymal stem cell-derived exosome provided by the invention can be used as a medicine for promoting angiogenesis and wound healing to be applied to diabetes wound treatment, and also provides a new strategy for treatment of other chronic wounds including burn.

The invention discloses a preparation method of photo-thermal-MMP dual-response matrix hydrogel and application of the photo-thermal-MMP dual-response matrix hydrogel in diabetic wound repair, and belongs to the technical field of biological medicine. According to the present invention, the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is composed of an amnion source photo-crosslinked matrix hydrogel, curcumin-loaded ZIF-8 nanoparticles and MMP2 peptide modified polydopamine, and the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is obtained; the ZIF-8 nanoparticles decline in an acid environment to release zinc ions, destroy bacterial cell membranes, enhance the permeability of a biological membrane and cooperatively kill drug-resistant bacteria in combination with a photothermal effect, Cur is loaded through ZIF-8 to form Cur-coated ZIF-8, then a Cur-coated ZIF-8-coated MMPDA / dAMG double-drug-loading system is constructed through an MMPDA medium, and a photothermal antibacterial, anti-inflammatory and regeneration-promoting multi-mode therapy is formed for a wound surface in the acid environment. Therefore, wound healing is accelerated.

The present invention discloses a chloroplast-sodium alginate microsphere and its preparation method and use. The preparation method of the chloroplast-sodium alginate microsphere comprises: mixing chloroplasts, sodium alginate and distilled water to obtain a mixed solution of chloroplasts and sodium alginate as a dispersed phase; dissolving a nonionic surfactant and CaI2 or CaCl2 in liquid paraffin to form an oil phase as a continuous phase; the dispersed phase and the continuous phase are cross-linked under the operation of a microfluidic device to form chloroplast-sodium alginate microspheres. The chloroplast-sodium alginate microspheres of the present invention have the ability to generate oxygen under illumination and are sustainable for up to two weeks. Chloroplasts are immunomodulated by reprogramming sugar metabolism to achieve anti-inflammatory and repair-promoting effects. The chloroplast-sodium alginate microspheres are suitable for wound repair, especially for chronic hypoxic wound repair similar to diabetic wounds.

The invention discloses a sugar response hydrogel wound dressing based on Prussian blue nano-enzyme and bacterial cellulose as well as a preparation method and application of the sugar response hydrogel wound dressing, and belongs to the field of medical dressings and biological materials. The sugar-responsive hydrogel wound dressing is good in mechanical property, and can be subjected to cascade reaction to play antibacterial and anti-inflammatory roles. The preparation method comprises the following steps: preparing Prussian blue nano-enzyme (PBNPs) by adopting a one-pot method and utilizing a self-polymerization reaction, and preparing a Prussian blue nano-enzyme bacterial cellulose compound by utilizing an isotonic adsorption method; glucose oxidase (GOx) is loaded on BC coated PBNPs by adopting an in-situ polymerization method, the sugar response hydrogel wound dressing based on PBNPs and BC is obtained, the preparation method is simple in process, and the obtained sugar response hydrogel wound dressing is good in stability, suitable for factory production and hopeful to be applied to diabetes wound treatment. Meanwhile, the PBNPs are applied to the field of diabetic wound dressings, and the application direction of the PBNPs is widened.

This invention discloses a method for preparing a pH / glucose dual-responsive chronic wound dressing containing frankincense essential oil, comprising: (1) using 4-formaldehyde phenylboronic acid as a crosslinking agent to form Schiff base and borate ester bonds with carboxymethyl chitosan and two different molecular weight hyaluronic acids, respectively, to prepare a hydrogel capable of filling skin surface wounds and performing dual dynamic covalent crosslinking adhesion, and freeze-drying for later use; (2) preparing frankincense essential oil into microcapsules by emulsification coagulation method, and freeze-drying for later use; (3) mixing the hydrogel with the freeze-dried powder of frankincense essential oil microcapsules, adding deionized water or PBS and stirring to reconstitute to form a pH / glucose dual-responsive hybrid hydrogel dressing coated with frankincense essential oil microcapsules. The composite dressing prepared by this invention has anti-inflammatory, antioxidant, antibacterial, and healing-promoting effects, is quick and easy to prepare, and has the ability to respond to the acidic / high-glucose microenvironment of diabetic wounds, and has important practical application value.

The invention provides an antibacterial hydrogel dressing for promoting healing of diabetic wounds and a preparation method of the antibacterial hydrogel dressing. The hydrogel is prepared by taking a combination of a specific amount of nano-silver and antibacterial peptide conjugate and adenovirus as an active component, the toxicity of silver ions is eliminated, the stability of antibacterial peptide is enhanced, meanwhile, the advantages of high replication capacity, stability, long-term expression and the like of the adenovirus are achieved, and the obtained hydrogel can remarkably promote microangiogenesis and has a good application prospect. The collagen regeneration is promoted, the cell proportion of new granulation tissues is improved, and the effect of repairing the diabetic wound is good. The preparation method is simple and suitable for industrial production.

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention belongs to the technical field of biomedical materials, and relates to a composite dressing with sequential response and functional synergy as well as preparation and application thereof, the composite dressing is of a double-layer composite structure, and comprises a PVA-GOx-MnO2 hydrogel layer and a temperature-sensitive micro-nanofiber membrane layer; the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into a polyvinyl alcohol solution under the condition of ice bath, and the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into the polyvinyl alcohol solution; the preparation method comprises the following steps: placing the temperature-sensitive micro-nano fiber membrane on a PVA-GOx-MnO2 hydrogel precursor solution or immersing the temperature-sensitive micro-nano fiber membrane in the PVA-GOx-MnO2 hydrogel precursor solution, carrying out crosslinking treatment, and demolding to obtain the composite dressing. The application is as follows: the material is used as a diabetic chronic wound repair material. The problems that an existing diabetes wound dressing is weak in microenvironment regulation and control capacity and the drug release time is not matched are solved; the preparation method is simple; the healing efficiency and the treatment safety of diabetic wounds are remarkably improved during application.