339 results about "Diabetic wound" patented technology

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention discloses a photo-thermal composite hydrogel patch and a preparation method and application thereof.The hydrogel patch is of a three-dimensional latticed porous structure formed by taking methylacryloyl gelatin (GelMA) and methylacryloyl hyaluronic acid (HAMA) as matrixes, loading niobium carbide nano-particles (Nb2CNPs) and vinipofen (VP) and conducting 3D printing or casting molding, the hydrogel patch is named as VP-Nb2C-coated GelMA / HAMA (VNGH) hydrogel patch for short. The VNGH has good photo-thermal antibacterial property, oxidation resistance and reepithelization promoting function, can significantly improve the quality of mouse diabetic wound healing, and provides a new thought and strategy for realizing scar-free healing of diabetic wounds.

The invention discloses a double-response collagen-based conductive hydrogel, a medicine and a preparation and application method of the double-response collagen-based conductive hydrogel. The preparation method of the double-response collagen-based conductive hydrogel comprises the following steps: carrying out chemical modification on polysaccharide through 3-aminophenylboronic acid, and then carrying out sodium periodate oxidation treatment to obtain a dialdehyde polysaccharide grafted aminophenylboronic acid copolymer; the preparation method comprises the following steps: performing hydrazide modification on collagen through adipic acid dihydrazide, and performing free radical polymerization with aniline to obtain a hydrazide collagen grafted polyaniline copolymer; the dialdehyde polysaccharide grafted aminophenylboronic acid copolymer and the hydrazide collagen grafted polyaniline copolymer are subjected to crosslinking, and the double-response collagen-based conductive hydrogel capable of responding to glucose and the pH value is obtained. Compared with single-response conductive hydrogel, the hydrogel can specifically respond to the microenvironment of chronic diabetic wound surfaces and release antibacterial and anti-inflammatory drugs, and high-quality healing of chronic wounds such as diabetic foot ulcers and the like is promoted.

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-layer multifunctional hydrogel microsphere for diabetic wounds as well as a preparation method and application of the multi-layer multifunctional hydrogel microsphere. The preparation method comprises the following steps: firstly, synthesizing a chitosan derivative with proper amino and aldehyde group contents, oxidized macromolecules and modified chitin; then preparing photocuring hydrogel microspheres by utilizing a microfluidic technology, and sequentially immersing the photocuring hydrogel microspheres into an oxidized polymer solution, a modified chitin solution, a tannic acid solution and a metal salt solution, so as to construct the hydrogel microspheres with a multifunctional layer structure. The invention further provides specific application intervals of parameters such as solvents used by various solutions, concentration ranges and soaking time. The preparation method provided by the invention has expansibility and universality, various types of microspheres can be constructed for sequential release of bioactive substances, and the requirements of tissue repair are met.

The invention discloses a bletilla striata polysaccharide hydrogel dressing and a preparation method and application thereof.The preparation method of the bletilla striata polysaccharide hydrogel dressing comprises the following steps that a carboxymethyl chitosan solution, a bletilla striata polysaccharide solution and cinnamyl aldehyde are mixed and stirred to be uniform at the temperature of 20-30 DEG C, a mixed solution system is prepared, and then standing is conducted to form gel; wherein the volume ratio of the carboxymethyl chitosan solution to the bletilla striata polysaccharide solution is (1-5): 1, and the concentration of the cinnamyl aldehyde in the mixed solution system is 0.05-5 wt%. The preparation process is simple, the raw materials are all green and natural sources, and the prepared hydrogel is good in biocompatibility, has good antibacterial, anti-inflammatory and angiogenesis promoting effects and can accelerate diabetic wound repair.

The invention discloses a high-activity injectable self-healing temperature-sensitive hydrogel dressing and a preparation method and application thereof, the high-activity injectable self-healing temperature-sensitive hydrogel dressing is obtained by forming a temperature-sensitive hydrogel through a two-dimensional nano material MXene aqueous solution and F127 under the hydrogen-bond interaction, combining an M2 macrophage source exosome on MXene under the electrostatic adsorption action, and wrapping the MXene through a network structure of the hydrogel. The preparation method is simple, an obtained sample is free of organic solution residues, reaction conditions are mild, operation is convenient, the raw material cost is low, the hydrogel dressing has temperature-sensitive, injectable and self-healing performance, the hydrogel dressing has temperature-sensitive and injectable performance, is sol at normal temperature, forms gel after making contact with skin and is convenient to smear and use on wounds, and experimental results prove that the hydrogel dressing has good application prospects. The hydrogel has good biocompatibility and relatively strong antibacterial performance, and can effectively play anti-inflammatory and vascular regeneration promoting roles in diabetic wounds and promote rapid repair of the diabetic wounds.

The invention discloses Janus microspheres co-loaded with melanin and tea polyphenol-magnesium as well as a preparation method and application of the Janus microspheres. The preparation method comprises the following steps: complexing tea polyphenol and magnesium ions under an alkaline condition to form nano-particles, and dissolving keratin in hair by using strong base to obtain melanin nano-particles; the two solutions are respectively mixed with methacryloyl collagen to prepare a solution as a mobile phase, vegetable oil is used as a dispersion phase solution, in a microfluidic device, a two-channel flow phase is coaxial and co-flow, the dispersion phase fluid disperses the mobile phase into monodisperse liquid drops by using a flow shear force, and the monodisperse liquid drops are subjected to ultrasonic dispersion. And curing the liquid drops under the irradiation of ultraviolet laser to form the Janus microspheres co-loaded with melanin / tea polyphenol-magnesium. By means of the microfluidic technology, high-uniformity preparation of the Janus microspheres co-loaded with melanin / tea polyphenol-magnesium is achieved, synergistic anti-oxidation and antibacterial treatment can be achieved, and the Janus microspheres can be applied to various biomedical applications with anti-oxidation and antibacterial requirements, such as promotion of healing of diabetic wounds, bacterial infectious wounds and the like.

The invention relates to the technical field of biological medicine, and particularly provides visual hydrogel based on multiple response mechanisms as well as a preparation method and application of the visual hydrogel. The preparation method comprises the following steps: (1) preparing an inclusion compound from beta-cyclodextrin, hydroxypropyl-beta-cyclodextrin and anthocyanin; (2) respectively preparing a polyvinyl alcohol / rhein solution and a mixed solution of quaternary ammonium salt chitosan / hyaluronic acid / kappa-carrageenan; and (3) initiating dynamic crosslinking through phenylboronic acid, constructing a multi-network structure by cooperating with tannic acid, the clathrate compound and genipin, and performing gradient temperature control curing to form the hydrogel. The hydrogel has the pH / temperature / ROS response characteristic, the wound surface microenvironment state can be fed back in real time by means of anthocyanin color change, meanwhile, hyaluronic acid / polyvinyl alcohol can construct a 3D microchannel structure, ordered arrangement of collagen is promoted, wound surface healing is accelerated, and the wound surface healing effect is improved. Therefore, the problems that direct loading of anthocyanin is unstable, and a single response mechanism is difficult to adapt to a complicated microenvironment of a diabetic wound surface are solved.

The composition is of a network structure and is composed of strontium ions and chlorogenic acid, and the weight ratio of the strontium ions to the chlorogenic acid is 1: (9-11). The composition and hydrogel are mixed to prepare a microneedle patch, and the microneedle patch is prepared into a medical instrument for wound repair. Through verification, the SrC-MPNs is continuously released by the SrC-MPNs (at) MN-PP, so that the substances are dissolved and absorbed into tissue fluid around a wound, and strontium ions and chlorogenic acid in the SrC-MPN can stably and continuously exert the effects of resisting bacteria and oxidation, promoting angiogenesis, promoting wound healing and the like. An in-vivo experiment result shows that the SrC-MPNs (at) MN-PP can promote in-vivo and in-vitro wound healing, promote M2 macrophage polarization, remove excessive ROS and promote angiogenesis and tissue regeneration so as to promote healing of diabetic wounds.

The present invention relates to the technical field of diabetes, and in particular to a controlled-release diabetic wound healing promoting treatment method, comprising the following treatment experimental steps: step S1: preparation of Hst1@PNPs; step S2: Coomassie brilliant blue staining; step S3: scanning and transmission electron microscopy detection; in the present invention, a slow-release Hst1 treatment method is constructed by encapsulating Hst1 into platelet membrane vesicles derived from autologous blood. On the one hand, the PNPs encapsulate the linear Hst1 peptide structure to prevent it from being rapidly degraded by high proteases in diabetic wound exudate, thereby improving bioavailability. On the other hand, the PNPs can adsorb excess LPS in the wound exudate, and cooperate with Hst1 to achieve anti-inflammatory and anti-oxidative stress effects. Subsequent cell and animal experiments further confirmed that Hst1@PNPs have the effects of anti-inflammatory, scavenging ROS, promoting re-epithelialization, collagen deposition and angiogenesis, thereby promoting diabetic wound healing.

The invention provides an endometrial mesenchymal stem cell-derived exosome and application thereof in preparation of a medicine for treating diabetic wounds, and belongs to the technical field of biological medicines. The endometrial mesenchymal stem cell-derived exosome provided by the invention can effectively promote HUVEC tube forming ability, promote wound angiogenesis of diabetic model mice, increase collagen deposition and reduce wound area, finally achieves the purpose of promoting healing of diabetic wounds, and solves the problem of limited treatment effect of exosomes in the prior art. The endometrial mesenchymal stem cell-derived exosome provided by the invention can be used as a medicine for promoting angiogenesis and wound healing to be applied to diabetes wound treatment, and also provides a new strategy for treatment of other chronic wounds including burn.

The invention discloses a preparation method of photo-thermal-MMP dual-response matrix hydrogel and application of the photo-thermal-MMP dual-response matrix hydrogel in diabetic wound repair, and belongs to the technical field of biological medicine. According to the present invention, the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is composed of an amnion source photo-crosslinked matrix hydrogel, curcumin-loaded ZIF-8 nanoparticles and MMP2 peptide modified polydopamine, and the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is obtained; the ZIF-8 nanoparticles decline in an acid environment to release zinc ions, destroy bacterial cell membranes, enhance the permeability of a biological membrane and cooperatively kill drug-resistant bacteria in combination with a photothermal effect, Cur is loaded through ZIF-8 to form Cur-coated ZIF-8, then a Cur-coated ZIF-8-coated MMPDA / dAMG double-drug-loading system is constructed through an MMPDA medium, and a photothermal antibacterial, anti-inflammatory and regeneration-promoting multi-mode therapy is formed for a wound surface in the acid environment. Therefore, wound healing is accelerated.

The present invention discloses a chloroplast-sodium alginate microsphere and its preparation method and use. The preparation method of the chloroplast-sodium alginate microsphere comprises: mixing chloroplasts, sodium alginate and distilled water to obtain a mixed solution of chloroplasts and sodium alginate as a dispersed phase; dissolving a nonionic surfactant and CaI2 or CaCl2 in liquid paraffin to form an oil phase as a continuous phase; the dispersed phase and the continuous phase are cross-linked under the operation of a microfluidic device to form chloroplast-sodium alginate microspheres. The chloroplast-sodium alginate microspheres of the present invention have the ability to generate oxygen under illumination and are sustainable for up to two weeks. Chloroplasts are immunomodulated by reprogramming sugar metabolism to achieve anti-inflammatory and repair-promoting effects. The chloroplast-sodium alginate microspheres are suitable for wound repair, especially for chronic hypoxic wound repair similar to diabetic wounds.

The invention discloses a sugar response hydrogel wound dressing based on Prussian blue nano-enzyme and bacterial cellulose as well as a preparation method and application of the sugar response hydrogel wound dressing, and belongs to the field of medical dressings and biological materials. The sugar-responsive hydrogel wound dressing is good in mechanical property, and can be subjected to cascade reaction to play antibacterial and anti-inflammatory roles. The preparation method comprises the following steps: preparing Prussian blue nano-enzyme (PBNPs) by adopting a one-pot method and utilizing a self-polymerization reaction, and preparing a Prussian blue nano-enzyme bacterial cellulose compound by utilizing an isotonic adsorption method; glucose oxidase (GOx) is loaded on BC coated PBNPs by adopting an in-situ polymerization method, the sugar response hydrogel wound dressing based on PBNPs and BC is obtained, the preparation method is simple in process, and the obtained sugar response hydrogel wound dressing is good in stability, suitable for factory production and hopeful to be applied to diabetes wound treatment. Meanwhile, the PBNPs are applied to the field of diabetic wound dressings, and the application direction of the PBNPs is widened.

This invention discloses a method for preparing a pH / glucose dual-responsive chronic wound dressing containing frankincense essential oil, comprising: (1) using 4-formaldehyde phenylboronic acid as a crosslinking agent to form Schiff base and borate ester bonds with carboxymethyl chitosan and two different molecular weight hyaluronic acids, respectively, to prepare a hydrogel capable of filling skin surface wounds and performing dual dynamic covalent crosslinking adhesion, and freeze-drying for later use; (2) preparing frankincense essential oil into microcapsules by emulsification coagulation method, and freeze-drying for later use; (3) mixing the hydrogel with the freeze-dried powder of frankincense essential oil microcapsules, adding deionized water or PBS and stirring to reconstitute to form a pH / glucose dual-responsive hybrid hydrogel dressing coated with frankincense essential oil microcapsules. The composite dressing prepared by this invention has anti-inflammatory, antioxidant, antibacterial, and healing-promoting effects, is quick and easy to prepare, and has the ability to respond to the acidic / high-glucose microenvironment of diabetic wounds, and has important practical application value.

The invention provides an antibacterial hydrogel dressing for promoting healing of diabetic wounds and a preparation method of the antibacterial hydrogel dressing. The hydrogel is prepared by taking a combination of a specific amount of nano-silver and antibacterial peptide conjugate and adenovirus as an active component, the toxicity of silver ions is eliminated, the stability of antibacterial peptide is enhanced, meanwhile, the advantages of high replication capacity, stability, long-term expression and the like of the adenovirus are achieved, and the obtained hydrogel can remarkably promote microangiogenesis and has a good application prospect. The collagen regeneration is promoted, the cell proportion of new granulation tissues is improved, and the effect of repairing the diabetic wound is good. The preparation method is simple and suitable for industrial production.

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention belongs to the technical field of biomedical materials, and relates to a composite dressing with sequential response and functional synergy as well as preparation and application thereof, the composite dressing is of a double-layer composite structure, and comprises a PVA-GOx-MnO2 hydrogel layer and a temperature-sensitive micro-nanofiber membrane layer; the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into a polyvinyl alcohol solution under the condition of ice bath, and the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into the polyvinyl alcohol solution; the preparation method comprises the following steps: placing the temperature-sensitive micro-nano fiber membrane on a PVA-GOx-MnO2 hydrogel precursor solution or immersing the temperature-sensitive micro-nano fiber membrane in the PVA-GOx-MnO2 hydrogel precursor solution, carrying out crosslinking treatment, and demolding to obtain the composite dressing. The application is as follows: the material is used as a diabetic chronic wound repair material. The problems that an existing diabetes wound dressing is weak in microenvironment regulation and control capacity and the drug release time is not matched are solved; the preparation method is simple; the healing efficiency and the treatment safety of diabetic wounds are remarkably improved during application.

The invention relates to the technical field of medicines, in particular to a medical dressing for promoting healing of a diabetic wound and a preparation method of the medical dressing. The medical dressing for promoting healing of the diabetic wound surface is prepared from the following raw materials in percentage by mass: 0.008 to 0.012 percent of stem cell exosome, 0.002 to 0.005 percent of oligopeptide-1, 0.004 to 0.01 percent of jasminin, 0.03 to 0.07 percent of modified CaSi2 nanosheets, 2 to 6 percent of hydroxypropyl chitosan, 0.1 to 0.5 percent of xanthan gum, 0.5 to 5 percent of glycerol and the balance of water. The medical dressing not only can promote wound healing, but also is beneficial to scar repair after wound healing.

The invention belongs to the technical field of medicines, and particularly relates to a hydrogel dressing for promoting healing of diabetic wounds as well as a preparation method and application of the hydrogel dressing. According to the hydrogel dressing for promoting healing of the diabetic wound, glycyrrhizic acid and S-allyl-L-cysteine are used as raw materials, a buffer solution is used as a dispersion medium, and the glycyrrhizic acid and S-allyl-L-cysteine are compounded and self-assembled by controlling the mass ratio of the raw materials, the reaction temperature, the reaction time and the pH value. The hydrogel shows tissue-like viscoelasticity characteristics, can adaptively deform along with wound movement, provides continuous protection for chronic wounds under dynamic physiological conditions, and has flexibility of adapting to dynamic requirements in different healing stages.

The invention relates to the technical field of biomedical materials, in particular to photo-thermal antibacterial healing-promoting hydrogel as well as a preparation method and application thereof. The preparation method of the hydrogel comprises the following steps: dispersing mesoporous polydopamine (MPDA) into a Tris-HCl solution, and then adding L-arginine to prepare L-MPDA; the preparation method comprises the following steps: fully dissolving 3-(aminomethyl) phenylboronic acid hydrochloride and 4-(4, 6-dimethoxytriazine-2-yl)-4-methylmorpholine hydrochloride into hyaluronic acid (HA), regulating the pH value to 6.5 by using alkali liquor to obtain phenylboronic acid modified hyaluronic acid mixed liquor, and dialyzing and freeze-drying to obtain HA-PBA. The preparation method comprises the following steps: dissolving metformin and purified L-MPDA in deionized water, sequentially adding purified HA-PBA and a polyvinyl alcohol (PVA) solution, and uniformly mixing to obtain the hydrogel. The hydrogel prepared by the invention has excellent hemostasis, antibacterial and adhesion properties. When the hydrogel is used as a hydrogel dressing for treating a diabetic wound surface, the healing speed of the diabetic wound surface can be effectively improved.

The invention relates to the technical field of biomedical materials, in particular to a microneedle patch for diabetes wound healing and a preparation method and application thereof. The microneedle patch comprises a substrate and a microneedle array arranged on the surface of the substrate, the substrate is prepared from a first raw material system comprising polyvinyl alcohol, phenylboronic acid modified oxidized hyaluronic acid, bromothymol blue and first drug micromolecules; the microneedle array is prepared from a second raw material system which comprises methylacryloylated gelatin, 2-(methylacryloyloxy) ethyl choline phosphate and platelet cell membrane wrapped MOF loaded second drug micromolecules. The microneedle patch has the characteristics of promoting angiogenesis, being excellent in antibacterial property and slow release, also has sensitive pH responsiveness and ROS responsiveness at the same time, can adjust the release dose of the medicine according to the wound surface microenvironment change (pH value change and ROS concentration change), can monitor the wound surface state in real time according to the wound surface pH value change indication, and is suitable for being popularized and used clinically.

The invention relates to the field of biological materials, in particular to modified recombinant III-type collagen hydrogel loaded with herba houttuyniae vesicles and application of the modified recombinant III-type collagen hydrogel loaded with the herba houttuyniae vesicles. The hydrogel is prepared from the herba houttuyniae vesicles, modified recombinant human-derived III-type collagen and polyvinyl alcohol, modified recombinant human-derived three-type collagen and polyvinyl alcohol are subjected to physical and chemical double-crosslinking to form collagen hydrogel, the collagen hydrogel is loaded with plant extracellular vesicles prepared from traditional Chinese medicine herba houttuyniae, and when the collagen hydrogel is applied to diabetic wounds, the hydrogel keeps a moist environment for the wounds; due to change of microenvironments such as active oxygen and pH in skin tissues, boric acid ester bonds in the collagen hydrogel can be promoted to be broken, so that the active oxygen is removed, and the wrapped herba houttuyniae vesicles are released into wounds.

The invention discloses a temperature-controlled anti-inflammatory and antibacterial skin wound dressing capable of promoting revascularization and a preparation method and application thereof, and relates to the technical field of medical materials, the preparation method comprises the following steps: S1, preparing calcium peroxide microspheres; s2, the poloxamer 407, the chitosan and the forsythiaside A are jointly dissolved in an acetic acid solution, and mixed gel is obtained; s3, dispersing calcium peroxide microspheres into the mixed gel to obtain the skin wound dressing capable of controlling temperature, resisting inflammation and bacteria and promoting revascularization. The traditional Chinese medicine active ingredient forsythiaside A is loaded, so that the healing efficiency of diabetic wounds is improved; due to the temperature-sensitive property of the gel, the gel can be better attached to the wound, the antibacterial property can inhibit microorganism breeding at the wound surface, the oxygen release capacity can improve the anoxic environment at the wound, and the loaded traditional Chinese medicine active ingredient forsythiaside A has the anti-inflammatory capacity, promotes cell regeneration at the wound and accelerates the repair process.

The invention belongs to the technical field of medicines, and discloses application of protein nano-selenium gel in preparation of a medicine for repairing wounds and preventing and treating scars. Compared with the nano-selenium gel prepared by the traditional method, the protein nano-selenium gel disclosed by the invention is better in stability and stronger in antioxidant, antibacterial and anti-inflammatory effects, has better functions in the aspects of repairing wound surfaces and diabetic wound surfaces and inhibiting scar formation, and can be well applied to preparation of medicines for repairing wound surfaces and preventing and treating scars.

The invention belongs to the technical field of biological medicine, and particularly discloses application of miR-184-3p in preparation of medicine for improving diabetes related tissue damage repair. The miR-184-3p has a nucleotide sequence as shown in SEQ ID NO. 1 (sequence identifier number 1). Researches find that miR-184-3p can regulate diabetic wound anti-inflammatory factors and play a role in wound repair, down-regulate the expression of inflammatory factors IFN-gamma, up-regulate the expression level of anti-inflammatory cytokines IL-4 and IL-13, especially regulate diabetic wound excessive inflammatory response and promote wound healing, and can be used for treating diabetic wounds. The miR-184-3p can be used as an active component for diabetes wound inflammation resistance or development of pharmaceutical preparations for promoting related tissue damage repair, and has a good application prospect.

The invention discloses a medicinal preparation for promoting healing of chronic diabetic wounds as well as a preparation method and application of the medicinal preparation, and relates to the technical field of medicinal preparations. According to the invention, a thrombin solution containing Se-CQDs and a fibrinogen solution containing GOX are mixed and reacted to prepare the pharmaceutical preparation for promoting healing of chronic wounds of diabetes. The pharmaceutical preparation for promoting healing of the chronic wound of diabetes can effectively remove excessive ROS at the chronic wound of diabetes and reduce the pH, glucose content and M1 / M2 macrophage proportion at the chronic wound, so that the high-glucose alkaline inflammation microenvironment of the chronic wound of diabetes is reversed, collagen deposition, revascularization and skin tissue reepithelization are promoted, and the healing of the chronic wound of diabetes is promoted. Therefore, healing of chronic diabetic wounds is promoted. Meanwhile, Se-CQDs and GOX are combined to prepare the pharmaceutical preparation, and the pharmaceutical preparation has a synergistic effect on promoting healing of chronic wounds of diabetes mellitus.

The invention provides a sunlight-excited multi-ROS (reactive oxygen species) response oxygen sensitization type hydrogel. The sunlight-excited multi-ROS response oxygen sensitization type hydrogel is prepared from a plant-derived nano capsule-like body (NT), an AQPP liposome (AL) and pluronic F127 serving as a hydrogel matrix. The invention also provides a preparation method of the hydrogel and application of the hydrogel in preparation of medicines for treating skin ulcers. The NTALG causes bacterial membrane function damage after illumination, has a strong antibacterial effect and can promote healing of diabetic wounds.

The invention belongs to the technical field of biomedical materials, and discloses a preparation method of nano composite hydrogel and application of the nano composite hydrogel in acceleration of healing of diabetic wounds. The invention constructs the hydrogel based on the epsilon-polylysine grafted by the oxidized hyaluronic acid and the phenylboronic acid and the ZIF-8. Due to the existence of the dynamic Schiff base, the boric acid ester bond and the coordination bond, the hydrogel has ideal mechanical property, strong viscosity, injectability, self-healing property and pH / ROS responsiveness. Due to the existence of chlorogenic acid and zinc ions, the hydrogel regulates macrophage phenotypes, relieves oxidative stress and accelerates healing of diabetic wounds through an NF-kappa B / JAK-STAT signal channel.

The invention provides a bismuth-based heterogeneous material for promoting healing of diabetic wounds and a preparation method and application thereof. The bismuth-based heterogeneous material comprises a bismuth-based porphyrin metal organic framework, and the surface of the bismuth-based porphyrin metal organic framework is modified with Au-Pt nanoparticles. According to the bismuth-based heterogeneous material disclosed by the invention, microenvironments of bacterial infection, high glucose, active oxygen damage, inflammatory disorder, pH rise and oxygen deficit of diabetic wounds are improved through multiple antibacterial effects and enzyme catalysis effects, so that the healing of the diabetic wounds is promoted.