189 results about "Diabetic wound" patented technology

The invention discloses a high-activity injectable self-healing temperature-sensitive hydrogel dressing and a preparation method and application thereof, the high-activity injectable self-healing temperature-sensitive hydrogel dressing is obtained by forming a temperature-sensitive hydrogel through a two-dimensional nano material MXene aqueous solution and F127 under the hydrogen-bond interaction, combining an M2 macrophage source exosome on MXene under the electrostatic adsorption action, and wrapping the MXene through a network structure of the hydrogel. The preparation method is simple, an obtained sample is free of organic solution residues, reaction conditions are mild, operation is convenient, the raw material cost is low, the hydrogel dressing has temperature-sensitive, injectable and self-healing performance, the hydrogel dressing has temperature-sensitive and injectable performance, is sol at normal temperature, forms gel after making contact with skin and is convenient to smear and use on wounds, and experimental results prove that the hydrogel dressing has good application prospects. The hydrogel has good biocompatibility and relatively strong antibacterial performance, and can effectively play anti-inflammatory and vascular regeneration promoting roles in diabetic wounds and promote rapid repair of the diabetic wounds.

The invention discloses a sugar response hydrogel wound dressing based on Prussian blue nano-enzyme and bacterial cellulose as well as a preparation method and application of the sugar response hydrogel wound dressing, and belongs to the field of medical dressings and biological materials. The sugar-responsive hydrogel wound dressing is good in mechanical property, and can be subjected to cascade reaction to play antibacterial and anti-inflammatory roles. The preparation method comprises the following steps: preparing Prussian blue nano-enzyme (PBNPs) by adopting a one-pot method and utilizing a self-polymerization reaction, and preparing a Prussian blue nano-enzyme bacterial cellulose compound by utilizing an isotonic adsorption method; glucose oxidase (GOx) is loaded on BC coated PBNPs by adopting an in-situ polymerization method, the sugar response hydrogel wound dressing based on PBNPs and BC is obtained, the preparation method is simple in process, and the obtained sugar response hydrogel wound dressing is good in stability, suitable for factory production and hopeful to be applied to diabetes wound treatment. Meanwhile, the PBNPs are applied to the field of diabetic wound dressings, and the application direction of the PBNPs is widened.

This invention discloses a method for preparing a pH / glucose dual-responsive chronic wound dressing containing frankincense essential oil, comprising: (1) using 4-formaldehyde phenylboronic acid as a crosslinking agent to form Schiff base and borate ester bonds with carboxymethyl chitosan and two different molecular weight hyaluronic acids, respectively, to prepare a hydrogel capable of filling skin surface wounds and performing dual dynamic covalent crosslinking adhesion, and freeze-drying for later use; (2) preparing frankincense essential oil into microcapsules by emulsification coagulation method, and freeze-drying for later use; (3) mixing the hydrogel with the freeze-dried powder of frankincense essential oil microcapsules, adding deionized water or PBS and stirring to reconstitute to form a pH / glucose dual-responsive hybrid hydrogel dressing coated with frankincense essential oil microcapsules. The composite dressing prepared by this invention has anti-inflammatory, antioxidant, antibacterial, and healing-promoting effects, is quick and easy to prepare, and has the ability to respond to the acidic / high-glucose microenvironment of diabetic wounds, and has important practical application value.

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention belongs to the technical field of medicines, and particularly relates to a hydrogel dressing for promoting healing of diabetic wounds as well as a preparation method and application of the hydrogel dressing. According to the hydrogel dressing for promoting healing of the diabetic wound, glycyrrhizic acid and S-allyl-L-cysteine are used as raw materials, a buffer solution is used as a dispersion medium, and the glycyrrhizic acid and S-allyl-L-cysteine are compounded and self-assembled by controlling the mass ratio of the raw materials, the reaction temperature, the reaction time and the pH value. The hydrogel shows tissue-like viscoelasticity characteristics, can adaptively deform along with wound movement, provides continuous protection for chronic wounds under dynamic physiological conditions, and has flexibility of adapting to dynamic requirements in different healing stages.

The invention provides a sunlight-excited multi-ROS (reactive oxygen species) response oxygen sensitization type hydrogel. The sunlight-excited multi-ROS response oxygen sensitization type hydrogel is prepared from a plant-derived nano capsule-like body (NT), an AQPP liposome (AL) and pluronic F127 serving as a hydrogel matrix. The invention also provides a preparation method of the hydrogel and application of the hydrogel in preparation of medicines for treating skin ulcers. The NTALG causes bacterial membrane function damage after illumination, has a strong antibacterial effect and can promote healing of diabetic wounds.

The invention relates to a dressing for promoting healing of diabetic wounds. Chitosan is dissolved in a hydrochloric acid solution at room temperature to obtain a chitosan solution; the preparation method comprises the following steps: dissolving loxoprofen in ethanol activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide / N-hydroxysuccinimide, dropwise adding into a chitosan solution, adding ethanol while stirring at a high speed by using a magnetic rod to form a transparent solution, and stirring at a low speed in the dark; air-drying the solution in a drying oven to form a solid film, and thoroughly washing the solid film in ethanol to obtain a chitosan-loxoprofen conjugate; the preparation method comprises the following steps: adding chitosan-loxoprofen into a phosphate buffer solution to form a chitosan-loxoprofen solution; dissolving genipin in a phosphate buffer solution to obtain a genipin solution, stirring and mixing the chitosan-loxoprofen solution and the genipin solution at a high speed, cross-linking, and freezing in a refrigerator to obtain the chitosan-loxoprofen / genipin sponge bracket. The dressing disclosed by the invention can effectively relieve chronic inflammation, remarkably improve skin tissue regeneration and stimulate angiogenesis.

This application relates to the field of medical image processing technology, and discloses a multimodal image recognition method and system for dynamic assessment of diabetic wound healing. The method includes acquiring infrared thermal imaging images, hyperspectral images, and visible light color images of a diabetic wound collected at the same time of visit; identifying the wound boundary between the diabetic wound and surrounding tissues in the visible light color image, and determining the central reference point of the visible light color image based on the wound boundary; establishing a virtual polar coordinate system based on the central reference point and preset anatomical reference markers; extracting tissue feature information characterizing the pathological state of the wound from the infrared thermal imaging image, hyperspectral image, and visible light color image, and mapping the spatial position of the tissue feature information in its corresponding image to the virtual polar coordinate system; normalizing the mapped tissue feature information, and calculating wound healing assessment indicators based on the normalized tissue feature information in the virtual polar coordinate system.

The application discloses a kind of by polydopamine mesoporous nanoparticles crosslinked sulfhydrylated hyaluronic acid hydrogel and its preparation method and application, with dopamine and 4-formylphenylboronic acid as monomer, with trimethylbenzene as and poloxamer as template agent, in weak alkaline (pH=8.4), oxygen exists in the environment, by the oxidation self-polymerization reaction between monomer Preparation of polydopamine nanoparticles with mesoporous structure.Then the water dispersion of polydopamine nanoparticles is mixed with sulfhydrylated hyaluronic acid solution according to certain proportion, and the hydrogel dressing with injectable property is prepared.The hydrogel is expected to be used as skin wound dressing for the treatment of diabetic wounds.

The invention relates to the technical field of biomedical materials, in particular to a thermoelectric bionic skin for promoting healing of diabetic wounds by restoring a biological electric field microenvironment, and a preparation method comprises the following steps: adding silver selenide nanoparticles into a 10% methacryloyl gelatin solution, putting the precursor solution into a mold, adding 0.25% phenyl (2, 4, 6-trimethyl-1, 3-pentanedione), and stirring for 20-30 minutes to obtain the thermoelectric bionic skin for promoting healing of diabetic wounds by restoring a biological electric field microenvironment. 2, 6-trimethylbenzoyl) lithium phosphate (LAP) photoinitiator is added, ultraviolet light curing forming is carried out, and after demolding, soaking is carried out to remove residual monomers and the initiator; wherein the drug-containing group is prepared by replacing water with an artemisinin solution, and Ag2Se-coated GelMA or Ag2Se-coated GelMA / ART composite hydrogel is obtained. The electric field generated by the thermoelectric effect in the invention activates a voltage-gated calcium ion channel, inhibits PHD2, and up-regulates an HIF-1 / VEGF-A signal axis, thereby promoting angiogenesis. Meanwhile, the artemisinin also plays an anti-inflammatory role.

The invention relates to hydrogel for promoting healing of diabetic wounds as well as a preparation method and application of the hydrogel, and belongs to the technical field of hydrogel dressings. The invention discloses an exosome hydrogel, which is characterized in that exosome is loaded in crosslinked aldehyde hyaluronic acid and carboxymethyl chitosan, the aldehyde hyaluronic acid is grafted with L-arginine, and the exosome is derived from bone marrow mesenchymal stem cells. L-arginine can release nitric oxide under the action of a diabetic wound surface or an inflammation microenvironment to achieve the effects of resisting bacteria and promoting wound surface recovery, and the exosome successfully avoids the risks of cell survival defects, vascular embolism, immunological rejection and the like while retaining powerful bioactive components. The product has excellent antibacterial activity, degradability, tissue adhesion, injectable performance and self-repairing performance, and can be suitable for wounds in different shapes.

This invention provides a bilayer programmed release hydrogel dressing based on PD-168077. The hydrogel dressing is formed by photocrosslinking a lower fast-release layer and an upper slow-release layer. The fast-release layer is formed by photocrosslinking and curing a first pregel solution containing a first concentration of methacrylamide hyaluronic acid, methacrylamide collagen, a photoinitiator, and PD-168077. The slow-release layer is formed by photocrosslinking and curing a second pregel solution containing a second concentration of methacrylamide hyaluronic acid, methacrylamide collagen, a photoinitiator, and PD-168077. This invention also provides a method for preparing the dressing and its application. This invention constructs a biomimetic dermal triple network structure through a photopolymerization strategy of "single formulation, dual concentration, dual curing," achieving programmed release of PD-168077 with a fast-then-slow release. This synergistic effect of the drug, including anti-inflammatory, antioxidant, and angiogenesis-promoting properties, along with matrix bioactivity, effectively promotes the healing of chronic diabetic wounds.

The present disclosure relates to a composition comprising a peptide having at least 90% sequence homology to a peptide of SEQ ID No. 1 and epidermal growth factor (EGF). It also relates to a process of preparing the composition and its use in treating wounds, including diabetic wound complications.

The invention belongs to the technical field of high polymer materials, and particularly relates to azidated carboxymethyl chitosan, glucose responsive polysaccharide-based hydrogel and a preparation method and application thereof. The azidation carboxymethyl chitosan is obtained by taking carboxymethyl chitosan and fluorosulfonyl azide as raw materials and carrying out azidation reaction. According to the method, the FSO2N3 directly reacts with the carboxymethyl chitosan to prepare the azidated carboxymethyl chitosan in one step for the first time, the reaction conditions are mild, the product purity is high, and a new technical route is provided for polysaccharide modification. Glucose-responsive polysaccharide-based hydrogel covalently linked with polyphenols is further prepared from the azidated carboxymethyl chitosan, has the performances of glucose-responsive release, oxidation resistance, bacteria resistance and cell migration promotion, and can effectively promote healing of diabetic wounds.

The invention relates to ROS (reactive oxygen species) / pH (potential of hydrogen) dual response type viscous fiber composite hydrogel as well as preparation and application thereof, amino-modified zeolite-loaded imidazate skeleton-8 (ZIF-8) nanoparticle short fibers and natural polyphenol drugs are introduced into double-dynamic cross-linked network hydrogel containing boric acid ester bonds and Schiff base bonds, and the ROS / pH dual response type viscous fiber composite hydrogel has ROS and pH response drug / Zn < 2 + > release characteristics; good antibacterial performance and DPPH removing performance are realized. The ZIF-8 / fiber composite hydrogel developed by the invention realizes multiple functions of good tissue viscosity, antibiosis and antioxidation, and can effectively regulate the wound microenvironment and promote the healing of rat diabetes infected wound.

The application discloses a wireless wearable ultrasonic driving electrotherapy device for promoting diabetic wound healing, which comprises a piezoelectric energy collection unit and a packaging layer for packaging the piezoelectric energy collection unit; the piezoelectric energy collection unit comprises a glycine-poly (caprolactone) piezoelectric nanofiber film and integrated metal molybdenum electrodes arranged on the upper and lower sides of the glycine-poly (caprolactone) piezoelectric nanofiber film; the integrated metal molybdenum electrodes comprise conductive electrodes and stimulating electrodes, and the upper and lower conductive electrodes are attached to the upper and lower sides of the glycine-poly (caprolactone) piezoelectric nanofiber film; the upper and lower stimulating electrodes extend out of the glycine-poly (caprolactone) piezoelectric nanofiber film. Experiments prove that the device can significantly accelerate wound healing, and the healing speed is obviously improved compared with a control group. The application shows the application potential of the flexible bioelectronic device as a new generation of functional wound dressing.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of a composition in the preparation of a drug for treating diabetic wounds. The composition comprises the following components: *Artemisia capillaris* exosomes, epi-suberol, and 3-hydroxymorinogenone, with a mass ratio of 1:0.1-0.25:0.02-0.05. In this composition, *Artemisia capillaris* exosomes can penetrate the thickened inflammatory exudate layer and glycosylated extracellular matrix of diabetic wounds, reshaping the wound's immune repair microenvironment, promoting fibroblast activation and proliferation, regulating collagen synthesis and deposition, and reducing scar hyperplasia. 3-hydroxymorinogenone can increase vascular endothelial growth factor, promote angiogenesis, and thus promote the repair and healing of diabetic wounds. The synergistic effect of *Artemisia capillaris* exosomes, epi-suberol, and 3-hydroxymorinogenone in this composition significantly shortens the healing cycle of difficult-to-heal diabetic wounds and improves the quality of wound healing.

The invention relates to a cell membrane coating Janus probiotic spore motor driven by a pathological microenvironment as well as a preparation method and application of the cell membrane coating Janus probiotic spore motor, and particularly provides the cell membrane coating Janus probiotic spore motor which is mainly driven by hydrogen peroxide (H2O2) in the pathological microenvironment of a diabetes wound. The cell membrane coating Janus spore motor provided by the invention has pathological microenvironment-driven efficient movement ability, inflammatory factor neutralizing ability and harmful pathogenic bacterium adhesion inhibition ability, and can efficiently inhibit inflammatory storm. The invention further provides a preparation method of the JSPM (at) M, and the method is simple, universal, low in production cost and high in stability.

The invention belongs to the technical field of hydrogel preparation, and relates to a preparation method and application of metabolism programming hydrogel, and the preparation method comprises the following steps: 1, oxidizing dextran Dex through sodium periodate NaIO4 to generate oxidized dextran ODex rich in aldehyde; 2, under the protection of nitrogen, carrying out amino dynamic Schiff base reaction of APTC and carrying out covalent binding with an aldehyde group of oxidized dextran ODex to form ODex-APTC; 3, mixing ODex-APTCC and CMC GOx solutions at room temperature to form hydrogel based on hemiacetal crosslinking and a hydrogen bond mechanism; according to the invention, autonomous'antibacterial repair 'dual-phase switching is realized through metabolic microenvironment reprogramming; in the stage of wound surface high-glucose infection, the hydrogel utilizes a diabetes wound surface high-glucose environment as an endogenous fuel, and glucose reduction, active oxygen mediated sterilization and microenvironment acidification are realized at the same time through reaction of glucose oxidase and glucose; when glucose oxidase reacts, the acid environment triggers a controlled H2S release program, and wound healing is accelerated by relieving oxidative stress and activating a regeneration signal channel.

The invention discloses an application of a Rap1 gene in preparation of a medicine for promoting healing of a diabetic wound, and further discloses an application of a recombinant vector, a recombinant microorganism and / or a transgenic cell line containing a nucleic acid molecule for coding the Rap1 in preparation of the medicine for promoting healing of the diabetic wound, and an application of the Rap1 in preparation of the medicine for promoting healing of the diabetic wound. The method has the advantages that the expression condition of the Rap1 in the diabetes wound tissue and the action and molecular mechanism of the Rap1 in the diabetes wound tissue are determined. Through in-vivo and in-vitro experiments, the influence of Rap1 on biological behaviors such as proliferation and adhesion of keratinocytes on the diabetic wound is researched; the molecular mechanism of the Rap1 for regulating and controlling the biological behaviors of the endothelial cells of the diabetic wound surface is deeply explored, the interaction relationship between the Rap1 and other signal channels is disclosed, and a basis is provided for developing new therapeutic drugs.

The invention belongs to the field of protein hydrogel, and relates to protein adhesive hydrogel for promoting diabetic oral soft tissue injury repair and a preparation method of the protein adhesive hydrogel. The hydrogel is prepared by mixing an aqueous solution of recombinant fusion protein and an aqueous solution of polyethylene glycol bissuccinimide succinate. The sequence of the recombinant fusion protein is as shown in SEQ ID NO: 1: [PAATAVSHTTHAP (VPGKG) 5] 36. The hydrogel disclosed by the invention has good biocompatibility and excellent adhesion performance, can form a lasting antibacterial barrier, and is electrostatically combined with free DNA (Deoxyribonucleic Acid) in NETs, so that inflammatory factor expression induced by the NETs is inhibited, and finally diabetes wound healing is accelerated.

The invention discloses a pH and ROS double-response hydrogel based on probiotic microcapsules combined with prebiotics and a preparation method and application thereof, and belongs to the field of biomedical materials, the hydrogel is composed of the probiotic microcapsules, ROS response release mangiferin fragments and pH sensitive hydrogel; specifically, amino groups rich in the surface of the probiotic gelatin microcapsule are crosslinked with aldehyde groups contained in oxidized dextran and 4-formylphenylboronic acid to form a Schiff base bond with pH response. Mangiferin is combined with a boric acid group in 4-formylphenylboronic acid through a catechol structure and is crosslinked in the hydrogel to form a boric acid ester bond with ROS (reactive oxygen species) response. According to the hydrogel prepared by the preparation method disclosed by the invention, the probiotic microcapsules and the ROS responsively released prebiotics are combined, so that the synergistic antibacterial and antioxidant effects are achieved, and the effect of treating diabetic wounds is achieved.

This invention belongs to the field of biomedical technology and relates to a drug-loaded vesicle for promoting the healing of diabetic wounds. The drug-loaded vesicle has a core-shell structure. The core contains siRNA targeting ALKBH5 mRNA; the shell is a hybrid membrane of human umbilical vein endothelial cell membrane and milk-derived extracellular vesicle membrane, with its surface embedded with arginine-rich lipopeptides, and at least some arginine exposed outside the hybrid membrane. The core-shell structure effectively protects the siRNA from degradation and reduces off-target interference. The hybrid membrane shell has good biocompatibility, immunogenicity, and low cytotoxicity. The exposed arginine enhances tissue penetration and cellular uptake. In vitro and in vivo experiments have confirmed that this drug-loaded vesicle has good cellular uptake efficiency, effectively downregulates ALKBH5 expression, and restores mRNA levels. 6 A-modification increases the stability of C2orf69 mRNA, improves endothelial cell function, significantly promotes the healing of diabetic wounds, and has good biocompatibility and safety.

The invention discloses an application of spirulina (SP) in preparation of a product for treating diabetes-related diseases. According to the application disclosed by the invention, spirulina is found to be capable of alleviating oxidative stress in cells, promoting macrophages to be converted from a proinflammatory M1 type to an anti-inflammatory M2 type, remodeling redox balance and inhibiting an inflammatory microenvironment. More importantly, it is found that spirulina can lower the level of advanced glycation end products (AGEs) to inhibit the glycosylation process, and healing of diabetic wounds and bone defects is accelerated. In addition, the invention also finds that the spirulina can promote proliferation, migration and vascularization of vascular endothelial cells in a high-glucose and high-fat microenvironment, and further accelerate healing of bone defects. On the basis, the invention provides application of spirulina in preparation of products for treating diabetes-related diseases.

The present application relates to a kind of polyoxovanadomolybdate nanoclusters and its preparation method and application.The chemical general formula of the polyoxovanadomolybdate nanocluster is Na6[V 10‑x Mo x O 28 ]·nH2O, wherein 1≤x≤4, n is the number of crystallization water, the value range is 8~12.The polyoxovanadomolybdate nanocluster provided by the present application is by Mo substitution part V atom in dodecanovanadate lattice, into lattice in substitutional doping form, on the basis of retaining parent body POV insulin-like activity, give treatment agent excellent antioxidant function, and through the combined action of antioxidant and insulin-like activity, realize the multiple regulation of diabetic wound microenvironment, promote wound healing.

The application discloses a konjac glucomannan microneedle patch for diabetic wound healing and a preparation method and application thereof, and belongs to the technical field of medical device preparation. The microneedle patch is composed of a needle tip array and a backing layer, wherein the needle tip array and the backing layer are both formed by an oxidized konjac glucomannan and a mixed aqueous solution of hyaluronic acid, and a CuGA-MOF material is added to the aqueous solution for making the microneedle patch. The microneedle patch provided by the application can treat diabetic wounds in a minimally invasive manner. In vitro and in vivo verification shows that the konjac glucomannan microneedle patch loaded with the CuGA-MOF can inhibit bacterial growth, remove active oxygen, regulate macrophage M2 polarization and promote angiogenesis, and can effectively realize rapid healing of diabetic wounds. In addition, the raw material of the microneedle patch is abundant, the preparation method is simple, the cost is low, the safety is high, and the microneedle patch has a wide application prospect in the field of treating infected wounds and chronic wounds.

The invention relates to the technical field of biological material manufacturing, in particular to vanadium-doped mesoporous bioactive glass powder for treating diabetic wounds and a preparation method of the vanadium-doped mesoporous bioactive glass powder. Adding an ammonia solution into the template agent solution to obtain a mixed solution; adding a silicon source, a phosphorus source, a calcium source and a vanadium source into the mixed solution in batches according to a preset interval time under a continuous stirring condition, continuously stirring and reacting for 5.5-24 hours, and centrifuging and collecting a precipitation product; and washing, roasting and grinding the obtained precipitation product to obtain the vanadium-doped mesoporous bioactive glass powder for treating diabetic wounds.

This invention provides the application of glucose-responsive nanohydrogels in the preparation of drugs for treating skin injuries. The method includes the following steps: mixing hyaluronic acid with a phenylboronic acid compound, adding 3-aminophenylboronic acid, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide, and performing a grafting reaction to obtain boric acid-modified hyaluronic acid; adding myricetin-3-O-galactoside, phospholipids, and cholesterol to an organic solvent, followed by thin-film hydration and ultrasonic treatment to obtain nanoliposomes; mixing the boric acid-modified hyaluronic acid with a polyvinyl alcohol solution, then adding the nanoliposome solution, and finally mixing to obtain the resulting nanohydrogel. The glucose-responsive nanohydrogel of this invention achieves adaptive drug release in the high glucose environment of diabetic wounds and regulates local metabolic homeostasis of the wound, especially the lysine degradation pathway in amino acid metabolism.

This invention relates to a glucose-responsive antibacterial nanocomposite microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. The glucose-responsive antibacterial nanocomposite microneedle patch is an integral soluble microneedle. The functional component of the microneedle material is a nanocomposite CIP / GOx@ZIF-8, which uses ZIF-8 as a nanocarrier, co-loading ciprofloxacin hydrochloride and glucose oxidase. This invention synthesizes CIP / GOx@ZIF-8 using a one-step self-assembly method, achieving co-loading of CIP HCl and GOx. Furthermore, this invention further prepares CIP / GOx@ZIF-8 into microneedles. The preparation method is simple, easy to use, and allows for self-administration. Topical application to skin wounds is safer and more effective than systemic administration, further improving drug delivery efficiency and delivering therapeutic agents deep into the wound, promoting wound healing. This invention combines multiple therapeutic mechanisms, significantly enhancing the antibacterial properties of the microneedles and achieving the treatment of bacterially infected diabetic wounds.