36 results about "Phosphatidyl ethanolamine" patented technology

The application discloses a liposome of a FOXO1 inhibitor AS1842856 and a preparation method and application thereof. The liposome comprises the following raw materials: phospholipid, cholesterol, phospholipid PEG derivative and AS1842856; wherein the proportion of the phospholipid, the cholesterol and the phospholipid PEG derivative (for example, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 (DSPE-PEG2000)) is preferably in the range of 10:0.2-2:0.5-5 (w / w); wherein the AS1842856 is wrapped in the liposome. The AS1842856 liposome of the application has excellent physical and chemical properties and has a wide application prospect in the field of fibrosis disease treatment.

The invention discloses a phototherapy nano-drug with mitochondrial / STAT3 protein double-site targeting. The phototherapy nano-drug is ATO / CR nano-particles formed by self-assembling a compound CR and atorvaquone under the action of distearoyl phosphatidyl ethanolamine-polyethylene glycol; the structure of the compound CR is shown as a formula I in the specification. The invention discloses an application of the phototherapy nano-drug with mitochondrial / STAT3 protein double-site targeting in preparation of drugs for treating tumors. The phototherapy nano-drug can target mitochondria and STAT3 protein in tumor cells through ATO, and can also passively target tumor sites through the high-permeability long-retention effect of nano-particles, so that more nano-therapeutic agents are enriched around tumors, and the curative effect is improved. The phototherapy nano-drug provided by the invention has good photothermal performance, can effectively enhance the PTT effect of gastric cancer, and exerts the tumor synergistic treatment ability by promoting cell apoptosis, inhibiting angiogenesis and hindering the cell cycle.

The invention relates to chlorotoxin-melittin nanoparticles as well as a preparation method and application thereof. The method comprises the following steps: weighing chlorotoxin, dissolving the chlorotoxin in a PBS buffer solution, adding 2-imino sulfane hydrochloride, fully mixing, and incubating at room temperature to form a CTX-SH solution; the preparation method comprises the following steps: weighing dimyristoyl phosphatidylcholine, adding a trichloromethane solution, uniformly mixing, then adding a cholesterol oleate solution and a phosphatidyl ethanolamine-polyethylene glycol 2000-maleimide solution, and sufficiently and uniformly mixing; drying the solution, resuspending with a PBS (Phosphate Buffer Solution), and fully dissolving by ultrasonic; adding a thiolated CTX-SH solution into the solution, incubating for 1-3 days, and carrying out ultrafiltration purification to form a CTX-NP nanoparticle solution; preparing a melittin solution, dropwise adding the melittin solution into the CTX-NP nanoparticle solution, incubating for 10-24 hours, and performing ultrafiltration purification. The invention has the advantages of targeting brain glioma, inhibiting the growth of in-situ brain glioma and improving the immunosuppressive microenvironment of brain glioma.

The invention provides an anti-neuroinflammation medicine as well as a preparation method and application thereof. The anti-neuroinflammation medicine comprises an active component and lipidosome, the active component is an inhibitor 4-aminobenzoyl hydrazine of myeloperoxidase; the liposome is prepared from phospholipid, cholesterol and distearoyl phosphatidyl ethanolamine-thioketal-polyethylene glycol; the method comprises the following steps: weighing lipidosome and 4-aminobenzoyl hydrazine, dissolving, and carrying out rotary evaporation to obtain a lipid membrane; adding a phosphate buffer solution into the lipid membrane, stirring in a water bath, performing ultrasonic treatment, filtering, centrifuging and purifying to obtain the medicine, according to the active oxygen response-based liposome delivery system disclosed by the invention, 4-aminobenzoyl hydrazine is encapsulated in liposome to form a drug, and after the drug enters a brain inflammation microenvironment area of the Alzheimer's disease, the active oxygen can break a thioketal bond, so that local release of 4-aminobenzoyl hydrazine is triggered, targeted inhibition of myeloperoxidase is realized, and the drug delivery effect is improved. The oxidative stress level is reduced, the neuroinflammation is relieved, and the cognitive impairment is effectively improved.

The application belongs to the field of biological medicine, and discloses a nano-lipid preparation for loading perfluorohexane and an antioxidant, wherein the nano-lipid preparation is a core-shell structure, the core-shell structure takes a phospholipid liposome membrane layer as an outer shell, and perfluorohexane and the antioxidant loaded in the outer shell serve as an inner core; the phospholipid liposome membrane layer is made of hydrogenated lecithin, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and cholesterol; and the application discloses an application of the nano-lipid preparation for loading perfluorohexane and the antioxidant in preparation of a medicine for treating myocardial infarction. The nano-lipid preparation has a suitable particle size, can be passively targeted and enriched to a myocardial infarction site, is stable, has a high encapsulation efficiency, and has a simple administration mode; after the lipid preparation is taken by myocardial cells, oxygen and the antioxidant are released, the oxygen can relieve an anoxic condition of a myocardial ischemia site, and the antioxidant can reduce ROS damage, both of which play a synergistic role, improve the survival rate of ischemic myocardial cells, and improve heart function.

The invention relates to the field of synthetic phospholipids, and discloses a method for preparing phosphatidyl ethanolamine synthetic phospholipids under catalysis of phospholipase D. The method comprises the following steps: A, completely dissolving PC in an organic solvent to obtain a PC solution; b, adding ethanolamine into the buffer solution, and adjusting the pH value in an ice bath; c, mixing a metal salt solution with phospholipase D to obtain a fermentation enzyme solution, pouring a buffer solution containing ethanolamine, and uniformly mixing to obtain a water phase; d, mixing the PC solution with a water phase to uniformly disperse PC, and carrying out hydrolysis reaction to obtain a PE crude product reaction solution; e, standing for layering to obtain a water layer, extracting the water layer, and concentrating the obtained organic layer to obtain a PE crude product concentrate; f, refining to obtain a PE crude product I; g, performing column chromatography separation and vacuum concentration to obtain a PE crude product II with the purity not lower than 98%; f, drying to obtain a PE finished product. The method is simple in process, good in enzyme specificity, mild in reaction condition, environment-friendly, high in yield and beneficial to large-scale production of enterprises.

The invention relates to a hypoxia response paclitaxel prodrug, a nano preparation as well as a preparation method and application thereof. The paclitaxel prodrug is a paclitaxel prodrug small molecule modified by a nitro derivative; the preparation method comprises the following steps: preparing the nano-drug by self-assembly of the paclitaxel prodrug, co-assembly of the paclitaxel prodrug and the photosensitizer or co-assembly of the paclitaxel prodrug and the polymer distearoyl phosphatidyl ethanolamine methoxy polyethylene glycol, so as to realize efficient loading and delivery of the drug and responsive release of the focus site. The prodrug obtained by the invention has a chemical structure for optimizing a connecting bond, can reduce the toxic and side effects of paclitaxel and quickly respond to a hypoxic microenvironment, and realizes quick and efficient release of an active drug in a tumor microenvironment.

The present application relates to a kind of biomimetic lung surfactant carrier, which is prepared from total fat and simulation peptide, in the total fat, the mass ratio of dipalmitoyl phosphatidylcholine, dipalmitoyl phosphatidylglycerol, dipalmitoyl phosphatidyl ethanolamine-polyethylene glycol 2000, cholesterol is 8-12:1:1:1;The lung surfactant simulation peptide is the simulation peptide of SP-B protein and / or the simulation peptide of SP-C protein.The biomimetic lung surfactant carrier has the advantages of high loading rate and high stability.The present application also relates to inhalation dosage form of drug for treating pulmonary arterial hypertension loaded by the biomimetic lung surfactant carrier, which makes the loaded drug obtain more uniform intra-pulmonary distribution, the inhalation drug is more close to natural lung surfactant in composition and physiological function, can delay the degradation of product, improve the utilization rate of drug, achieve good pulmonary arterial hypertension treatment effect.

The application discloses a high-performance composite aluminum film packaging bag and a preparation method thereof, and belongs to the technical field of packaging bags. The packaging bag has a three-layer structure, and comprises, from outside to inside, an aluminized film layer, an intermediate film layer and a heat-seal film layer. The aluminized film layer, the intermediate film layer and the heat-seal film layer are connected through a two-component polyurethane adhesive. The two-component polyurethane adhesive comprises a main agent and a curing agent. The main agent contains a Ti3AlC2 / SiO2 composite material modified by distearoyl phosphatidyl ethanolamine-polyethylene glycol-silane and loaded with menthol. Through optimization of the composition of the adhesive, the adhesive not only realizes the close connection between the layers, but also greatly improves the adhesion of the adhesive under high-temperature and high-humidity conditions, thereby guaranteeing the cooking resistance of the packaging bag. In addition, the packaging bag has certain heat storage performance, can better maintain the stability of the internal temperature, and optimizes the use experience of consumers.

The application discloses a preparation method and application of copper porphyrin merocyanine nanoparticles converted from photodynamic and photothermal effects to chemical dynamic effects. The copper porphyrin merocyanine molecule is prepared through a meso-substitution reaction of hydroxyl copper porphyrin and heptamethine merocyanine, and nanoparticles are formed through co-assembly with distearoyl phosphatidylcholine and distearoyl phosphatidyl ethanolamine-polyethylene glycol. Under light conditions, the copper porphyrin merocyanine nanoparticles have good photodynamic and photothermal effects. During the light process, the gradual degradation of merocyanine leads to the depolymerization of nanoparticles, and the loose structure promotes the opening of the chemical dynamic performance, so that the switching from photodynamic / photothermal effects to chemical dynamic effects is realized. The copper porphyrin merocyanine nanoparticles prepared by the application have good application prospects in the fields of biological tracing, efficient tumor killing and the like.

The invention discloses a preparation method of chitosan surface modified extracellular vesicles, which specifically comprises the following steps: S1, dissolving chitosan in water, adjusting the pH value to be acidic, and completely dissolving the chitosan to obtain a chitosan solution with the concentration of 10mg / mL; s2, adding N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride into the solution obtained in the step S1, and uniformly stirring and fully mixing the N-hydroxysuccinimide and the 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride; s3, the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 is dissolved in formamide, and a distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 solution with the concentration being 1 mg / mL is prepared; s4, uniformly mixing the solution obtained in the step S2 and the solution obtained in the step S3, and stirring at room temperature for 12 hours; s5, transferring the mixed solution obtained in the step S4 into a dialysis bag with the specification of 3.5 KDa, and dialyzing in pure water for 24 hours; and S6, co-incubating the dialyzed solution obtained in the step S5 with an extracellular vesicle solution, and self-assembling to form the extracellular vesicle with the chitosan surface modified. The chitosan surface modified extracellular vesicles prepared by the method disclosed by the invention have relatively high encapsulation efficiency on hydrophobic drugs such as fucoxanthin and the like and relatively high stability in various environments. The method is suitable for embedding and delivering various bioactive substances, and has an important application prospect in the aspect of active substance delivery.

The invention discloses a polycarbonate composite material as well as a preparation method and application thereof, and relates to the technical field of high polymer materials. The invention provides a polycarbonate composite material. The polycarbonate composite material is prepared from the following components in parts by weight: 70 to 95 parts of polycarbonate resin, 0.5 to 5.5 parts of polyolefin resin, 2 to 13 parts of phosphorus flame retardant, 1 to 9 parts of flexibilizer, 0.08 to 1.2 parts of hydrolysis-resistant agent and 0.04 to 0.52 part of lubricant, the lubricating agent is distearoyl phosphatidyl ethanolamine and / or dipalmitoyl phosphatidyl ethanolamine. The specific polyolefin resin, the hydrolysis-resistant agent, the lubricant and the like are selected, so that the PC composite material which has good appearance and can keep excellent mechanical properties and flame retardance in a high-temperature and high-humidity environment at the same time is prepared.

The invention relates to the technical field of biological preparation, in particular to FACOD (at) DSPE nano-particles, a preparation method and application of the FACOD (at) DSPE nano-particles in animal antibiosis / sterilization, the preparation method comprises the steps that 1, a compound IV and a compound COD are used, then N, N-diisopropylethylamine is added, the mixture is mixed and stirred overnight at the room temperature, extraction, drying, filtering, separation and purification are conducted through a separating funnel, and FACOD is obtained; and (2) wrapping the FACOD by using distearoyl phosphatidyl ethanolamine-methoxyl polyethylene glycol, so as to construct the FACOD (at) DSPE (Distearoyl Phosphatidyl Ethanolamine) nano particles. Compared with the prior art, the prepared FACOD (at) DSPE nano-particles with the light response characteristic can synergistically release FA with the yield of about 85.02% and CO with the yield of 50.3% under the illumination condition, the sterilization rate on escherichia coli is 91.36-99.80%, the sterilization rate on staphylococcus aureus is 94.11-99.85%, and the sterilization rate on drug-resistant staphylococcus aureus is 86.83-98.92%; and the FACOD (at) DSPE nanoparticles have the characteristics of low toxicity to cells and mice and good biological safety.

The invention relates to a Raman image probe molecule with photothermal conversion capability and a preparation and a preparation method thereof. The Raman image probe takes thiophene coupled benzo-bis-thiadiazole as a parent nucleus, and has specific Raman shift and good photothermal conversion efficiency. In a gathering state, after being irradiated by near-infrared light (700-900 nm), the material can generate a characteristic Raman scattering signal, can convert light energy into heat energy, and has a good photothermal conversion property. The nano preparation is prepared from distearoyl phosphatidyl ethanolamine-polyethylene glycol and a derivative thereof or albumin or a polyethylene glycol block copolymer and the Raman image probe, and has Raman scattering signal and photothermal conversion performance. The prepared nano preparation can generate strong characteristic Raman scattering signals under the irradiation of near-infrared light and can convert light energy into heat energy. The nano preparation can be applied to tumor living body Raman spectrum imaging and photothermal therapy, and realizes tumor diagnosis and treatment integrated precise therapy.

The application relates to the technical field of lipid microbubble, and is a plasmid-carrying cationic lipid microbubble and a preparation method thereof. The plasmid-carrying cationic lipid microbubble is composed of a lipid shell and a gas core, and is obtained by the following method: after di-stearoyl phosphatidylcholine, di-stearoyl phosphatidyl ethanolamine-polyethylene glycol 2000, dipalmitoyl phosphatidylcholine and DC cholesterol are mixed according to a required proportion and dissolved in chloroform, the formed lipid membrane is subjected to vacuum extraction, hydration, vacuum extraction again, gas replacement and mechanical oscillation to obtain the plasmid-carrying cationic lipid microbubble. The prepared cationic lipid microbubble has high electric potential and small particle size, has high plasmid carrying capacity and carrying rate, has the advantages of low price, simple operation, storage convenience and the like, and provides a reference for the preparation of other gene-carrying microbubbles.

The invention discloses preparation of a liver-targeted fucoxanthin nano delivery system based on probiotic vesicles and application of the liver-targeted fucoxanthin nano delivery system in improvement of bioavailability, and belongs to the field of biological medicine. Pure mycoderm fragments are obtained by combining a differential centrifugation-tangential flow filtration system, the pure mycoderm fragments are treated by an ultrasonic-assisted technology to form complete membrane nano-vesicles, galactosamine with sialic acid glycoprotein receptor targeting ability is modified to distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-carboxyl through an amide reaction, and the sialic acid glycoprotein receptor targeting galactosamine nano-vesicles are prepared. The hepatic parenchymal cell sialoglycoprotein receptor targeting ligand is obtained. And co-incubating the hepatocyte sialoglycoprotein receptor targeted nano-vesicle with the nano-vesicle to obtain the hepatocyte sialoglycoprotein receptor targeted nano-vesicle. The hydrophobic food functional factors or drugs are encapsulated in the nano-vesicles, so that the water solubility, oxidation resistance, environmental stability and gastrointestinal digestive system stability of the hydrophobic food functional factors or drugs can be improved, and the bioavailability of blood and liver of oral administration of the hydrophobic food functional factors or drugs can be improved.

The application discloses a kind of loaded manganese doped ceria nanoscale enzyme's Pachyman hydrogel and its preparation method and application, it is related to biomedical technical field.The preparation method is first prepared by high-temperature thermal decomposition method fat-soluble manganese doped ceria nanoscale enzyme, then it is surface-modified using distearoyl phosphatidyl ethanolamine to obtain water-soluble nanoscale enzyme;Polyvinyl alcohol solution is mixed uniformly with Pachyman powder, water-soluble nanoscale enzyme, borax solution is added to crosslink and form hydrogel, after freeze-room temperature processing alternately, it is obtained.The energy storage modulus of the prepared hydrogel is 120-350 Pa at pH 6.5-7.2, 37 ℃, and the adhesion is greater than or equal to 8.5 kPa.The hydrogel prepared by the application has good biocompatibility, can efficiently remove active oxygen, relieve intestinal oxidative stress, and has suitable adhesion and degradation characteristics, can be administered by enema, and be used for preparing drugs for treating irritable bowel syndrome.

The invention belongs to the technical crossing field of semiconductors and biological nanomaterials, and particularly relates to biocompatible perovskite fluorescent nanoparticles and a preparation method thereof.The preparation method comprises the steps that brominated acid and oleylamine are mixed in a solvent to prepare a zwitterionic ligand solution, and a Pb source and an HBr solution are mixed to prepare a premixed solution; mixing and stirring the premixed solution, CsAc and the zwitterionic ligand solution, and then carrying out solid-liquid separation to obtain the aqueous-phase perovskite quantum dots, the preparation method comprises the following steps: dissolving water-phase perovskite quantum dots and distearoyl phosphatidyl ethanolamine-polyethylene glycol in an organic solvent, and then heating and evaporating to obtain a lipid dry film; immersing the dry lipid membrane in pure water, and carrying out deep mixing treatment to obtain a liposome emulsion; and extruding the liposome emulsion through a micron-sized filter membrane to obtain the perovskite fluorescent nanoparticles. The prepared perovskite fluorescent nanoparticles have good water stability and biocompatibility and are suitable for biological cell imaging, a large dose of toxic chemical reagents and / or inert reaction environment conditions are not needed in the preparation process, and the problem that commercialization of water stability and biocompatibility of the perovskite fluorescent nanoparticles is difficult to achieve is solved.

The invention relates to the technical field of preparation and application of tumor diagnosis and treatment probes, and discloses an orthogonal near-infrared light driven imaging / membrane targeted photodynamic therapy probe and a method thereof.The surfaces of rare earth down-conversion nanoparticles are modified with mesoporous silicon doped with photosensitizer pheophorbide A and perfluorocarbon through a sol-gel method; and mixing the meso-porous silicon modified down-conversion nanoparticles with distearoyl phosphatidyl ethanolamine-PEG (Polyethylene Glycol) to finally obtain the orthogonal near-infrared light driven imaging / membrane targeted photodynamic therapy probe. The prepared nano-probe can achieve orthogonal near-infrared light driven imaging and photodynamic therapy under 980 nm laser and 808 nm laser respectively, a reasonable orthogonal near-infrared light excitation system is formed, meanwhile, solution, cell and living body level experiments show that the nano-probe is good in biocompatibility and good in photodynamic therapy effect, and the application range of the nano-probe is widened. The pyroptosis can be efficiently induced through a membrane targeting photodynamic therapy, and excellent treatment performance is shown in vitro and in vivo.

The invention discloses a nano composite material for emitting light and measuring temperature in a near-infrared NIR-IIb region as well as a preparation method and application of the nano composite material. Raw materials of the composite material comprise rare earth nanoparticles and distearoyl phosphatidyl ethanolamine-polyethylene glycol, the rare earth nanoparticles comprise a core layer and a shell layer coating the core layer, the structural general formula of the core layer is NaEr1-ATmAF4, the structural general formula of the shell layer is NaLuF4, and A is larger than or equal to 0 and smaller than or equal to 0.1. The nano composite material disclosed by the invention is high in temperature measurement repeatability and good in temperature measurement stability, a luminescence emission peak is positioned in an optimal imaging region (NIR-IIb), and the imaging resolution of deep tissues can be remarkably enhanced. Therefore, the composite material disclosed by the invention becomes an ideal material for promoting the development of a living body deep tissue high-precision temperature measurement imaging technology based on the unique luminescence characteristic and stable temperature measurement performance, and has huge application potential in the fields of biomedical imaging and temperature measurement.

The invention discloses anti-inflammatory nanoparticles targeting neutrophil as well as a preparation method and application of the anti-inflammatory nanoparticles. The anti-inflammatory nano-particle for targeting the neutrophil, disclosed by the invention, is prepared from the following components: hyperbranched polyphosphate rich in thioether bonds, distearoyl phosphatidyl ethanolamine-polyethylene glycol and 2-((2, 3-bis (oleoyloxy) propyl) dimethyl ammonium) ethyl hydrogen phosphate. The anti-inflammatory nano-particles can efficiently target neutrophils of inflammatory lungs, can effectively remove active oxygen of the inflammatory lungs, relieve inflammatory reaction and reduce damage caused by lung inflammation, and are suitable for large-scale industrial production and application.

The invention discloses a near-infrared two-region aza-BODIPY photosensitizer capable of reversibly capturing and releasing singlet oxygen as well as a preparation method and application of the near-infrared two-region aza-BODIPY photosensitizer. According to the photosensitizer, a singlet oxygen carrier (2-pyridone, anthracene or 1, 4-dimethylnaphthalene) is used as a response unit, a triphenylamine-thiophene group is used as an electron donor, and an aza-BODIPY mother nucleus structure is modified. Wherein the singlet oxygen carrier can capture singlet oxygen generated by illumination through [4 + 2] cycloaddition reaction to generate endoperoxide; the latter releases singlet oxygen again through a reverse Diels-Alder reaction in a dark environment. A nano diagnosis and treatment agent constructed by self-assembly of the photosensitizer and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 through a nano precipitation method can capture singlet oxygen at a tumor site and release singlet oxygen for a long time under a dark condition under the irradiation of 808 nm laser; the technical bottlenecks that singlet oxygen is short in service life in a tumor microenvironment and needs a light source to continuously irradiate a photosensitizer are effectively overcome, and a new strategy is provided for enhancing the depth and curative effect of photodynamic therapy.

The present application belongs to the technical field of anti-tumor drug design, and particularly relates to a nanoparticle containing cisplatin-linoleic acid and SN38-linoleic acid, wherein the nanoparticle is formed by wrapping cisplatin-linoleic acid and SN38-linoleic acid with an amphiphilic polymer, and the amphiphilic polymer is distearoyl phosphatidyl ethanolamine-polyethylene glycol. By wrapping cisplatin-linoleic acid and SN38-linoleic acid prodrug molecules with the amphiphilic polymer, the water solubility of the drug molecules can be improved, and the in-vivo synchronous delivery and transportation of the cisplatin-linoleic acid and SN38-linoleic acid prodrug molecules can be realized, so that the synergistic anti-tumor effect can be achieved. In the mechanism, it is proved that the SN38-linoleic acid prodrug in the co-wrapped nanoparticle can not only cause DNA damage, but also inhibit the DNA damage repair protein Rad51, so as to increase the drug efficacy of the cisplatin-linoleic acid prodrug in drug-resistant tumors, and achieve the best tumor killing effect.

The invention belongs to the technical field of medicine, and relates to a quercetagetin self-microemulsion and a preparation method thereof.The quercetagetin self-microemulsion comprises oil, a surfactant and a cosurfactant and is characterized by further comprising a quercetagetin phospholipid complex; the quercetagetin and phospholipid complex is prepared from quercetagetin and phospholipid according to the mass ratio of 1: (0.2 to 5); the phospholipid is selected from one or more of phosphatidylcholine and phosphatidyl ethanolamine. According to the quercetagetin self-microemulsion disclosed by the invention, the quercetagetin and the phospholipid are combined through an intermolecular acting force to form a cell membrane bionic phospholipid complex, and the phospholipid complex increases the lipophilicity of molecules and promotes transcellular and intracellular transportation ways; according to the quercetagetin self-microemulsion, the synergistic effect between the phospholipid complex and the quercetagetin is utilized, so that the solubility and the permeability are remarkably improved.

The application discloses a cocaine esterase-loaded W / O / W nanoemulsion and a preparation method and application thereof, wherein the nanoemulsion is composed of cocaine esterase, a phosphate buffer, soybean phospholipid, vitamin E polyethylene glycol succinate, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and medium-chain triglyceride; the preparation method comprises the following steps: preparing an inner water phase; preparing an oil phase; slowly injecting the inner water phase into the oil phase under shearing to obtain a W / O primary emulsion; slowly adding the primary emulsion into an outer water phase under shearing, and obtaining a secondary emulsion through high-speed shearing; and performing high-pressure homogenization on the secondary emulsion to obtain a final W / O / W nanoemulsion. The cocaine esterase is combined with the nanoemulsion for the first time, the nanoemulsion prepared through the method has the effects of improving the temperature stability of the cocaine esterase and prolonging the in-vivo half-life, and can be applied to the rescue or prevention of cocaine acute poisoning.

The invention discloses a lipidosome gold nano composite material and a preparation method thereof. The preparation method comprises the following steps: preparing 25 nm gold particles by adopting a sodium citrate reduction method, and preparing a lipidosome with the diameter of about 142 nm and good monodispersity by adopting an ethanol injection method; a sample in which the volume ratio of a gold nano solution to a liposome solution is 1: 5 is selected for SERS detection; detailed analysis on the spectrum proves the existence of three main components, namely cholesterol, phosphatidylcholine and phosphatidyl ethanolamine, and finds that lipid molecules interact with gold nanoparticles through head groups; a strong vibration peak repeatedly appearing at 2120 cm <-1 > in a sample test is selected as a characteristic peak of a sample, the reliability and repeatability of liposome structure analysis by using the plasmon gold nanoparticles are further studied, a result shows that an SERS signal has good uniformity and repeatability, and the RSD of the local SERS signal is only 15.9%; on the basis of good uniformity and repeatability, the SERS hotspot map of the sample is also researched, and a prospect is provided for quantitative analysis.

The invention provides a preparation method of an mPEG5000-DPPE sodium salt, in which 1, 2-dipalmitoyl-s-glycerol-3-phosphatidyl ethanolamine-N-[methoxyl (polyethylene glycol)-5000] sodium salt, called mPEG5000-DPPE sodium salt for short, dibenzyl phosphate is used as a phospholipid head to introduce phosphorus atoms, so that the use of DPPE as an intermediate is avoided, a deprotection group is not needed, and the preparation method is simple and easy to implement. Phosphorus oxychloride is used for generating an intermediate dichlorophosphate, so that the generation of impurities is reduced, and the total yield is improved; the preparation method has the advantages of high reaction selectivity, reduction of the generation of side reactions and by-products, reduction of the reaction risk and cost, realization of amplified production of the mPEG5000-DPPE sodium salt, provision of high-quality pharmaceutical excipients for promotion to the market and better development of more domestic novel preparations, and provision of a brand-new and feasible industrial production route.

The invention discloses a cabazitaxel micelle and a preparation method thereof. The cabazitaxel micelle disclosed by the invention is prepared from cabazitaxel and polyethylene glycol derivatized phospholipid, the polyethylene glycol derivatized phospholipid comprises a polyethylene glycol part and a phospholipid part, and the phospholipid part is di (C12-C24 fatty acyl) phosphatidyl ethanolamine; the polydispersity coefficient of the micelle is not higher than 0.09. The cabazitaxel micelle provided by the invention has one or more of the following advantages: the product has uniform particle size, good stability, low impurity content, high release rate, short redissolution time and good clinical medication convenience.

The invention relates to the technical field of medicine, and discloses a nuciferine-based nano preparation for targeting digestive tract tumors and a preparation method thereof.The nuciferine-based nano preparation for targeting digestive tract tumors comprises nuciferine, 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidylcholine, cholesterol, cholesterol hemisuccinate, 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidylcholine, cholesterol hemisuccinate, 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidylcholine, cholesterol hemisuccinate, 1 the lipid vesicle is composed of 1, 2-dioleoyl-sn-glycerol-3-phosphatidyl ethanolamine and distearoyl phosphatidyl ethanolamine-polyethylene glycol (2000)-cyclo (essence-glycerol-radix asparagi-styrene-acrylic-lysine) polypeptide, and the lipid vesicle is composed of 2, 2-dioleoyl-sn-glycerol-3-phosphatidyl ethanolamine and distearoyl phosphatidyl ethanolamine-polyethylene glycol (2000)- The preparation method of the nuciferine-based digestive tract tumor targeting nano preparation comprises the steps of film dispersion, hydration, high-pressure extrusion and purification. The active targeting effect of the cyclo (essence-glycerol-asparagi-styrene-acrylic-lysine) polypeptide ligand is utilized. Synergistic release of drugs in a tumor microenvironment is achieved, the targeting efficiency and local drug concentration of nuciferine are improved, and the anti-tumor effect is enhanced.

The invention provides a cyanine derivative compound, a nano particle and a preparation method and application of the cyanine derivative compound and the nano particle, and belongs to the technical field of chemical synthesis and biomedicine. The structural formula of the cyanine derivative conjugate is as shown in formula 1. According to the invention, a small molecule compound is biologically encapsulated into an amphiphilic polymer distearoyl phosphatidyl ethanolamine-polyethylene glycol to form nanoparticles. The cyanine derivative provided by the invention has absorption in a near-infrared region, can realize SDT / PDT / PTT multi-mode combined treatment of thrombus, and can also be used as a chemiluminescent substrate to act with active oxygen for imaging, thereby realizing integration of diagnosis and treatment.