13 results about "Phosphatidyl ethanolamine" patented technology

The application discloses a liposome of a FOXO1 inhibitor AS1842856 and a preparation method and application thereof. The liposome comprises the following raw materials: phospholipid, cholesterol, phospholipid PEG derivative and AS1842856; wherein the proportion of the phospholipid, the cholesterol and the phospholipid PEG derivative (for example, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 (DSPE-PEG2000)) is preferably in the range of 10:0.2-2:0.5-5 (w / w); wherein the AS1842856 is wrapped in the liposome. The AS1842856 liposome of the application has excellent physical and chemical properties and has a wide application prospect in the field of fibrosis disease treatment.

The invention discloses a phototherapy nano-drug with mitochondrial / STAT3 protein double-site targeting. The phototherapy nano-drug is ATO / CR nano-particles formed by self-assembling a compound CR and atorvaquone under the action of distearoyl phosphatidyl ethanolamine-polyethylene glycol; the structure of the compound CR is shown as a formula I in the specification. The invention discloses an application of the phototherapy nano-drug with mitochondrial / STAT3 protein double-site targeting in preparation of drugs for treating tumors. The phototherapy nano-drug can target mitochondria and STAT3 protein in tumor cells through ATO, and can also passively target tumor sites through the high-permeability long-retention effect of nano-particles, so that more nano-therapeutic agents are enriched around tumors, and the curative effect is improved. The phototherapy nano-drug provided by the invention has good photothermal performance, can effectively enhance the PTT effect of gastric cancer, and exerts the tumor synergistic treatment ability by promoting cell apoptosis, inhibiting angiogenesis and hindering the cell cycle.

The application belongs to the field of biological medicine, and discloses a nano-lipid preparation for loading perfluorohexane and an antioxidant, wherein the nano-lipid preparation is a core-shell structure, the core-shell structure takes a phospholipid liposome membrane layer as an outer shell, and perfluorohexane and the antioxidant loaded in the outer shell serve as an inner core; the phospholipid liposome membrane layer is made of hydrogenated lecithin, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and cholesterol; and the application discloses an application of the nano-lipid preparation for loading perfluorohexane and the antioxidant in preparation of a medicine for treating myocardial infarction. The nano-lipid preparation has a suitable particle size, can be passively targeted and enriched to a myocardial infarction site, is stable, has a high encapsulation efficiency, and has a simple administration mode; after the lipid preparation is taken by myocardial cells, oxygen and the antioxidant are released, the oxygen can relieve an anoxic condition of a myocardial ischemia site, and the antioxidant can reduce ROS damage, both of which play a synergistic role, improve the survival rate of ischemic myocardial cells, and improve heart function.

The invention relates to the field of synthetic phospholipids, and discloses a method for preparing phosphatidyl ethanolamine synthetic phospholipids under catalysis of phospholipase D. The method comprises the following steps: A, completely dissolving PC in an organic solvent to obtain a PC solution; b, adding ethanolamine into the buffer solution, and adjusting the pH value in an ice bath; c, mixing a metal salt solution with phospholipase D to obtain a fermentation enzyme solution, pouring a buffer solution containing ethanolamine, and uniformly mixing to obtain a water phase; d, mixing the PC solution with a water phase to uniformly disperse PC, and carrying out hydrolysis reaction to obtain a PE crude product reaction solution; e, standing for layering to obtain a water layer, extracting the water layer, and concentrating the obtained organic layer to obtain a PE crude product concentrate; f, refining to obtain a PE crude product I; g, performing column chromatography separation and vacuum concentration to obtain a PE crude product II with the purity not lower than 98%; f, drying to obtain a PE finished product. The method is simple in process, good in enzyme specificity, mild in reaction condition, environment-friendly, high in yield and beneficial to large-scale production of enterprises.

The application discloses a high-performance composite aluminum film packaging bag and a preparation method thereof, and belongs to the technical field of packaging bags. The packaging bag has a three-layer structure, and comprises, from outside to inside, an aluminized film layer, an intermediate film layer and a heat-seal film layer. The aluminized film layer, the intermediate film layer and the heat-seal film layer are connected through a two-component polyurethane adhesive. The two-component polyurethane adhesive comprises a main agent and a curing agent. The main agent contains a Ti3AlC2 / SiO2 composite material modified by distearoyl phosphatidyl ethanolamine-polyethylene glycol-silane and loaded with menthol. Through optimization of the composition of the adhesive, the adhesive not only realizes the close connection between the layers, but also greatly improves the adhesion of the adhesive under high-temperature and high-humidity conditions, thereby guaranteeing the cooking resistance of the packaging bag. In addition, the packaging bag has certain heat storage performance, can better maintain the stability of the internal temperature, and optimizes the use experience of consumers.

The invention relates to the technical field of biological preparation, in particular to FACOD (at) DSPE nano-particles, a preparation method and application of the FACOD (at) DSPE nano-particles in animal antibiosis / sterilization, the preparation method comprises the steps that 1, a compound IV and a compound COD are used, then N, N-diisopropylethylamine is added, the mixture is mixed and stirred overnight at the room temperature, extraction, drying, filtering, separation and purification are conducted through a separating funnel, and FACOD is obtained; and (2) wrapping the FACOD by using distearoyl phosphatidyl ethanolamine-methoxyl polyethylene glycol, so as to construct the FACOD (at) DSPE (Distearoyl Phosphatidyl Ethanolamine) nano particles. Compared with the prior art, the prepared FACOD (at) DSPE nano-particles with the light response characteristic can synergistically release FA with the yield of about 85.02% and CO with the yield of 50.3% under the illumination condition, the sterilization rate on escherichia coli is 91.36-99.80%, the sterilization rate on staphylococcus aureus is 94.11-99.85%, and the sterilization rate on drug-resistant staphylococcus aureus is 86.83-98.92%; and the FACOD (at) DSPE nanoparticles have the characteristics of low toxicity to cells and mice and good biological safety.

The application discloses a kind of loaded manganese doped ceria nanoscale enzyme's Pachyman hydrogel and its preparation method and application, it is related to biomedical technical field.The preparation method is first prepared by high-temperature thermal decomposition method fat-soluble manganese doped ceria nanoscale enzyme, then it is surface-modified using distearoyl phosphatidyl ethanolamine to obtain water-soluble nanoscale enzyme;Polyvinyl alcohol solution is mixed uniformly with Pachyman powder, water-soluble nanoscale enzyme, borax solution is added to crosslink and form hydrogel, after freeze-room temperature processing alternately, it is obtained.The energy storage modulus of the prepared hydrogel is 120-350 Pa at pH 6.5-7.2, 37 ℃, and the adhesion is greater than or equal to 8.5 kPa.The hydrogel prepared by the application has good biocompatibility, can efficiently remove active oxygen, relieve intestinal oxidative stress, and has suitable adhesion and degradation characteristics, can be administered by enema, and be used for preparing drugs for treating irritable bowel syndrome.

The invention relates to the technical field of preparation and application of tumor diagnosis and treatment probes, and discloses an orthogonal near-infrared light driven imaging / membrane targeted photodynamic therapy probe and a method thereof.The surfaces of rare earth down-conversion nanoparticles are modified with mesoporous silicon doped with photosensitizer pheophorbide A and perfluorocarbon through a sol-gel method; and mixing the meso-porous silicon modified down-conversion nanoparticles with distearoyl phosphatidyl ethanolamine-PEG (Polyethylene Glycol) to finally obtain the orthogonal near-infrared light driven imaging / membrane targeted photodynamic therapy probe. The prepared nano-probe can achieve orthogonal near-infrared light driven imaging and photodynamic therapy under 980 nm laser and 808 nm laser respectively, a reasonable orthogonal near-infrared light excitation system is formed, meanwhile, solution, cell and living body level experiments show that the nano-probe is good in biocompatibility and good in photodynamic therapy effect, and the application range of the nano-probe is widened. The pyroptosis can be efficiently induced through a membrane targeting photodynamic therapy, and excellent treatment performance is shown in vitro and in vivo.

The present application belongs to the technical field of anti-tumor drug design, and particularly relates to a nanoparticle containing cisplatin-linoleic acid and SN38-linoleic acid, wherein the nanoparticle is formed by wrapping cisplatin-linoleic acid and SN38-linoleic acid with an amphiphilic polymer, and the amphiphilic polymer is distearoyl phosphatidyl ethanolamine-polyethylene glycol. By wrapping cisplatin-linoleic acid and SN38-linoleic acid prodrug molecules with the amphiphilic polymer, the water solubility of the drug molecules can be improved, and the in-vivo synchronous delivery and transportation of the cisplatin-linoleic acid and SN38-linoleic acid prodrug molecules can be realized, so that the synergistic anti-tumor effect can be achieved. In the mechanism, it is proved that the SN38-linoleic acid prodrug in the co-wrapped nanoparticle can not only cause DNA damage, but also inhibit the DNA damage repair protein Rad51, so as to increase the drug efficacy of the cisplatin-linoleic acid prodrug in drug-resistant tumors, and achieve the best tumor killing effect.

The invention belongs to the technical field of medicine, and relates to a quercetagetin self-microemulsion and a preparation method thereof.The quercetagetin self-microemulsion comprises oil, a surfactant and a cosurfactant and is characterized by further comprising a quercetagetin phospholipid complex; the quercetagetin and phospholipid complex is prepared from quercetagetin and phospholipid according to the mass ratio of 1: (0.2 to 5); the phospholipid is selected from one or more of phosphatidylcholine and phosphatidyl ethanolamine. According to the quercetagetin self-microemulsion disclosed by the invention, the quercetagetin and the phospholipid are combined through an intermolecular acting force to form a cell membrane bionic phospholipid complex, and the phospholipid complex increases the lipophilicity of molecules and promotes transcellular and intracellular transportation ways; according to the quercetagetin self-microemulsion, the synergistic effect between the phospholipid complex and the quercetagetin is utilized, so that the solubility and the permeability are remarkably improved.

The invention provides a cyanine derivative compound, a nano particle and a preparation method and application of the cyanine derivative compound and the nano particle, and belongs to the technical field of chemical synthesis and biomedicine. The structural formula of the cyanine derivative conjugate is as shown in formula 1. According to the invention, a small molecule compound is biologically encapsulated into an amphiphilic polymer distearoyl phosphatidyl ethanolamine-polyethylene glycol to form nanoparticles. The cyanine derivative provided by the invention has absorption in a near-infrared region, can realize SDT / PDT / PTT multi-mode combined treatment of thrombus, and can also be used as a chemiluminescent substrate to act with active oxygen for imaging, thereby realizing integration of diagnosis and treatment.

The invention discloses an oral drug nano-carrier capable of realizing lymphatic transport as well as a preparation method and application thereof, the oral drug nano-carrier is prepared from polylactic acid-glycolic acid copolymer, platycodon grandiflorum fatty acid, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and soya bean lecithin according to the mass ratio of (10-20): (5-15): (1-3): (0-5) in sequence, and the oral drug nano-carrier is prepared by mixing the polylactic acid-glycolic acid copolymer, the platycodon grandiflorum fatty acid, the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and the soya bean lecithin. The nano-particle dispersion liquid with the average particle size smaller than 300 nm is prepared by combining a nano-precipitation method and a post-insertion method. Experiments prove that the oral drug nano-carrier not only can realize lymphatic transport and remarkably improve oral bioavailability, but also can remarkably improve lung enrichment, has important significance and application value for solving the problem of low lung enrichment of oral indissolvable drugs, can be used as a carrier of the indissolvable oral drugs, and has wide application prospects. Especially a drug carrier for treating lung diseases.

The invention discloses a preparation method for realizing safe and effective antibacterial gene therapy of colorectal cancer by combining antibiosis and anti-tumor, and belongs to the field of drug carrier materials. The preparation method comprises the following steps: modifying lauric acid (LA) resisting fusobacterium nucleatum (Fn) and a targeting molecule 4-carboxyphenylboronic acid (PBA) into low-molecular-weight polyethyleneimine (OEI), and self-assembling with LA to obtain LA-OLP (AFs); the AFs is compounded with an anti-angiogenesis gene (sFlt-1), and the AFs and distearoyl phosphatidyl ethanolamine-methoxy polyethylene glycol (DSPE-mPEG) are co-assembled to obtain the nano particle LA (at) OLP / sFlt-1 / DSPE-mPEG (AFGTs-PEG). The method has the advantages of low cost and simple and feasible process flow, can be widely applied to the fields of materials science, biology, medicine and the like, and cooperates with antibacterial and anti-angiogenic gene therapy to enhance gene therapy on colorectal cancer.