The invention discloses a polyene phosphatidyl choline injection and the preparation method. The components and the ratio (portion) of the polyene phosphatidyl choline injection of the invention is: 465 portion of polyene phosphatidyl choline injection, 88 portion of benzyl zlcohol, 50 to 800 portion of glycocholic acid, cholic acid or tween-80, 50 to 80 portion of alcohol, propanediol or glycerin, 15 to 200 portion of sodium hydroxide or sodium carbonate, 0.5 to 5 portion of 2,6-D-itert-butyl-p-cresol, 0.8 to 8 portion of Tertiary butyl-4-hydroxyl anisole, 3 to 25 portion of Vitamin E by weight. The polyene phosphatidyl choline injection of the invention has good clarity, high stability, simple preparation process and easy operation.