50 results about "Gliadin" patented technology

The application belongs to the field of SOD preparation, and discloses a preparation method and device of SOD resistant to pepsin degradation, which comprises the following steps: S1 powder preparation: dissolving SOD fermentation pure liquid in a buffer solution with pH of 5.2-7.8, adding protective agent and tea yellow in sequence, stirring, filtering, and placing in a freeze dryer to prepare freeze-dried powder; S2 drying; S3 tabletting; S4, coating liquid preparation: dissolving wheat gliadin and soybean protein in a mixed solution prepared from water and ethanol with a volume ratio of 1:1, wherein the mass ratio of the wheat gliadin and the soybean protein is 0.5-1:1, adding glycerol, stirring for 20 min by using a magnetic stirrer, and ultrasonicating for 40 s; S5, coating: placing the tablets in a coating machine, setting air inlet volume, air inlet temperature, pan rotating speed, atomization rate, fan face pressure, and mortar flow, until the coating weight increases to 3%-10% in mass fraction and the coating thickness is 20-50 μm, and then drying and cooling. The prepared SOD is not easy to be photolyzed and can resist gastric acid degradation to ensure the complete activity of the SOD.

The invention provides a wheat gliadin detection method, and belongs to the technical field of biology. The detection method comprises the following steps: mixing a sample to be detected with an ethanol solution to obtain a mixture; performing water bath on the mixture to obtain a mixed extract; centrifuging the mixed extract to obtain a supernatant; adding a reductive alkylation reagent into the supernate to obtain a reaction product; adding alkaline trypsin into the reaction product to carry out enzymolysis reaction to obtain an enzymolysis product; a first characteristic peptide fragment and a second characteristic peptide fragment as shown in SEQ ID NO: 1 in the sequence table are respectively used as markers, and the wheat gliadin is measured by the enzymolysis product through ultra-high performance liquid chromatography-tandem mass spectrometry by utilizing the markers. The method is simple in process and capable of efficiently detecting the wheat gliadin.

A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.

The invention discloses application of a ZmEREB93 gene knockout biological material in cultivation of high-yield and high-quality corn, and relates to the technical field of biology. The ZmEREB93 gene knockout strain is constructed, homozygous seeds of the ZmEREB93 gene knockout strain are subjected to seed test analysis, and it is found that corn kernels of the knockout strain are remarkably superior to a wild type in the aspects of the grain length, the grain width, the hundred-grain weight and the single plant yield. In the early, middle and late periods of grain filling, the corn grains of the knockout strain are obviously superior to a wild type control in shape and endosperm structure. Further determination of starch content and protein content shows that the starch content of seeds of the knockout strain is significantly higher than that of a wild type control group, the alcohol-soluble protein content is also significantly increased, and the total protein content is significantly higher than that of a control group. Therefore, the ZmEREB93 gene has an important application value in cultivation of a high-yield and high-quality new corn variety.

The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in regulating and controlling the protein content of plant seeds, and belongs to the technical field of molecular biology. The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in at least one of regulation and control of protein content of plant seeds, creation of transgenic plants with dominant protein content and breeding of plant varieties with dominant protein content. The result of the embodiment of the invention shows that the seed protein content of a TaMYB46L-5A overexpression plant is obviously reduced, the seed protein content of a TaMYB46L-5A mutant plant is increased, and the contents of prolamin and glutenin are obviously increased. Therefore, the TaMYB46L-5A can negatively regulate and control the protein content of the plant seeds, and plays an important role in plant breeding.

The invention discloses a preparation method of specific yolk immunoglobulin (IgY) for resisting gliadin. The preparation method comprises the following steps: immunizing and inoculating experimental laying hens, monitoring health behaviors, collecting eggs, and separating, extracting and purifying the IgY. The IgY prepared on the basis of the method can serve as a food base material to be added into low-gluten food (such as whole wheat bread and noodles), and the allergy risk caused by intake of a small amount of gluten is reduced through specific binding of the IgY and gliadin; patients are allowed to properly ingest gluten-containing food, diet diversity is improved, nutrient imbalance is avoided, and the life quality of the patients is improved. As the IgY has good thermal stability, the IgY purified by the method can still tolerate mild heating (such as baking and cooking) in food processing, and can be widely applied to various forms of products such as baked food, meal replacement powder, liquid beverage and the like; meanwhile, the acid-base stability of the antibody can ensure that the antibody keeps activity in a stomach environment after oral administration and finally reaches the intestinal tract to play a role, and the limitation that the conventional antibody is easy to digest and degrade after oral administration is broken through.

The invention belongs to the technical field of immunotherapy of allergic diseases, and provides a method for identifying dominant B cell epitopes and T cell epitopes in wheat omega-5 prolamin. According to the identification method provided by the invention, an immunoinformatics tool, a proliferation test, a degranulation test and the like are comprehensively applied, and the cell proliferation capacity, cell factor release, IgG / IgE binding capacity and degranulation capacity of T / B cell epitopes are evaluated. Results show that the T cell epitopes T1 and T2 sharing the same sequence are relatively strong in multiplication capacity, can up-regulate Th2 related cell factors, but do not have degranulation activity. Three B cell peptide fragments B3, B4 and B9 which share a common motif region QQXPQQQ (X = F, L) can promote IgG / IgE binding and degranulation capabilities. These findings would contribute to the development of epitope-based immunotherapies against wheat allergy individuals.

The invention provides a preparation method of a mixed biomass carbon coated silicon-based negative electrode material. Biomass carbon is mainly derived from prolamin, glutenin and high-activity yeast. Under the catalysis of high-activity yeast, prolamin and glutenin meet water to form a net-shaped structure, and the flexibility of the carbonized porous negative electrode material can be adjusted through different addition proportions, so that the flexible porous nitrogen-doped carbon-coated silicon-based negative electrode material can be obtained, the problem of volume expansion in the electrochemical process of a silicon negative electrode is relieved, and meanwhile, the conductivity is improved; the excellent electrochemical performance is shown. The porous silicon-based negative electrode material with controllable flexibility is obtained through drying and high-temperature annealing, the method is simple, effective and low in cost, and the prepared composite silicon-based negative electrode material is excellent in performance and suitable for large-scale production.

The invention discloses a gluten specific alpaca polyclonal antibody and a double antibody-streptavidin enzyme-linked immunosorbent assay (ELISA) bran quality inspection test kit thereof. The detection kit provided by the invention takes an alpaca polyclonal antibody pAb as a capture antibody and biotinylated pAb as a detection antibody to carry out ELISA detection on gluten in a food matrix, and a novel double antibody-streptavidin ELISA detection method is constructed based on the detection kit. The detection method has good sensitivity and specificity, the EC50 value of alcohol-soluble protein is 51.21 ng / mL, the detection limit is as low as 0.07 ng / mL, the detection limit of the method is 4000 times lower than that of an existing R5 antibody method, and the sensitivity is better; when the alpaca polyclonal antibody pAb prepared by the invention is used for detection, the detection cost is reduced, reliable technical support is provided for gluten allergen detection, and the alpaca polyclonal antibody pAb is suitable for clinical and scientific research application of immunoassay kits, immune test strips and the like.

The preparation method comprises the following steps: mixing vital gluten and 65% ethanol according to a mass ratio of 1: 30, and stirring and extracting in a water bath kettle at 35 DEG C for 3 hours to dissolve alcohol-soluble protein in the vital gluten in the ethanol; after standing, centrifuging for 5 minutes by using a centrifugal machine under the condition of 3,500 r / min, taking the precipitate, repeating the steps for dissolving, centrifuging the solution for 5 minutes, and taking the precipitate, namely the glutenin aggregate. Dissolving the glutenin aggregate at 30 DEG C for 120 minutes by using 0.1% sodium hydroxide alkali liquor, standing at 50 DEG C for 12 hours, and regulating the pH value to be neutral to generate precipitate; and centrifuging for 5 minutes to obtain a precipitate which is alkali-soluble glutenin. Amylose and alkali-soluble glutenin are mixed according to the mass ratio of 1: 8-1: 10, drinking water accounting for 3%-5% of the starch amount is added, mixing is conducted for 30-50 min at the temperature of 30-35 DEG C, and the disulfide bond content of the alkali-soluble glutenin is reduced by 80% or above.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

The present invention relates to a method for producing 1B or 1D omega-5 gliadin-deleted wheat and wheat produced thereby, and, specifically, to a method for producing and selecting 1B or 1D omega-5 gliadin gene-deleted wheat by using radiation. The method for producing 1B or 1D omega-5 gliadin-deleted wheat, according to the present invention, enables the production of a radiation breeding-based non-GMO mutant wheat or wheat breeding parental line while retaining agronomic traits similar to those of a wild-type wheat variety, and thus wheat with reduced toxicity to wheat-dependent exercise-induced anaphylaxis (WDEIA) can be produced for use in the production of processed wheat foods and the like, or a parental line for breeding wheat varieties with reduced toxicity to WDEIA can be produced.

The invention belongs to the field of battery materials, and discloses a nickel positive electrode material and a preparation method and application thereof.The preparation method comprises the steps that a polycrystalline precursor of NixM1-x (OH) 2, a lithium source, glutenin and gliadin are ground and mixed, and mixed powder is obtained; mixing the solvent and the mixed powder to obtain slurry, and drying to obtain a mixture; and pre-sintering the mixture in an oxygen atmosphere, and then heating and roasting for a certain time to obtain solid particles, namely the nickel-based positive electrode material. By adding glutenin and gliadin and combining with a roasting process, primary crystal grains of a polycrystalline precursor of NixM1-x (OH) 2 continuously grow in the roasting process, so that the prepared nickel-based positive electrode material is of a submicron single-crystal structure, the specific surface area of the positive electrode material is reduced, the possibility of side reaction is reduced, and the service life of the positive electrode material is prolonged. The structural stability of the positive electrode material can be further improved, the cycle performance of the battery is finally improved, and the volume energy density of the battery is increased.

This invention discloses the application of corn protein coagulants or suspensions formed through liquid-liquid phase separation in bio-based adhesives or coatings. The preparation includes: dissolving corn gliadin or crude corn protein powder in a 70-90% v / v ethanol aqueous solution, removing impurities, adjusting the ethanol concentration to 40-60% v / v with water, and inducing phase separation to obtain coagulants; or directly adding corn gliadin to a 40-60% v / v ethanol aqueous solution and stirring to form coagulants. The resulting product is used as a bio-based adhesive for bonding sheet or paper-based materials, or as a coating to impart water / oil barrier properties to paper-based materials. This invention utilizes corn protein coagulants to achieve excellent adhesive performance and water resistance without crosslinking agents. The product is completely biodegradable, providing a green solution for replacing traditional plastic coatings and solving sheet bonding problems.

The application discloses a preparation method of antioxidant and pro-coagulation double-function sponge, and comprises the following steps: preparing an ethanol solution, adding corn alcohol-soluble protein to dissolve in the ethanol solution, adding deionized water with the same volume as the ethanol solution to perform reverse precipitation and ultrasonic dispersion, removing half volume of the solution in the dispersion liquid by rotary evaporation, adding glutaraldehyde aqueous solution to perform cross-linking, and obtaining cross-linking pro-coagulation nano-particle dispersion liquid; dissolving gelatin in hot water, stirring and dissolving at a low speed, adding glutaraldehyde, improving the rotating speed to perform chemical cross-linking, stopping foaming until uniform and stable foam is obtained, and obtaining foaming gelatin solution; pouring the obtained foaming gelatin solution into the cross-linking pro-coagulation nano-particle dispersion liquid, and obtaining antioxidant / pro-coagulation double-function sponge after freeze-drying; the volume fraction of the ethanol solution is 60-70%; the concentration of the corn alcohol-soluble protein solution is 10-50 mg / mL; and the volume ratio of the cross-linking pro-coagulation nano-particle dispersion liquid and the gelatin solution is 1:10-1:90.

And removing prolamin in the vital gluten by using a conventional alcohol extraction method to obtain glutenin. When the alkali-soluble glutenin is prepared, the mixing ratio of glutenin to a 0.1% NaOH solution is 1: 25 (g: mL), stirring and dissolving are performed at 30 DEG C for 120 minutes, and then centrifugation is performed to obtain the alkali-soluble glutenin. The method comprises the following steps: mixing amylose with a 20% NaCl solution according to a ratio of 1: 5 (g: mL), carrying out eutectic crystallization at 4 DEG C for 36 h, carrying out water washing for 3-5 times, and carrying out centrifugation to obtain the eutectic amylose. The prepared alkali-soluble glutenin and the eutectic amylose are respectively added according to the proportions of 8% and 2%-10% of the flour to prepare mixed powder, the addition amounts of the yeast and the salt are respectively 1-5% and 0.5-3% of the amount of the mixed powder, and the fried dough twist dough is prepared. And fermenting at 35-45 DEG C for 10-15 minutes, and kneading into strips for forming. And frying at the temperature of 150-170 DEG C for 8-10 minutes to obtain the fried dough twist with reduced oil content.

The invention discloses a primer probe combination for detecting wheat and peanut allergens based on a dual Proofman-LMTIA technology and application of the primer probe combination, and belongs to the technical field of molecular detection. The primer probe combination comprises an XM-F1, an XM-B1, an XM-LB1, an HS-F2, an HS-B2, an HS-LB2, an XM-Probe1 and an HS-Probe2 which are as shown in SEQ ID NO. 1 to 8. The invention further discloses a kit for detecting the content of the XM-B1 in the kit. On the basis of a Proofman-LMTIA technology, specific primers and probes for wheat and peanut allergens are designed, a rapid detection method for wheat allergen gamma-gliadin and peanut allergen Arah 2.01 protein is established, and the method is used for detecting whether the wheat and peanut allergens are mixed in the links of production, processing and circulation of processed food. And technical support is provided for improving effective supervision of food supervision departments on allergen-containing products.

The present disclosure provides systems and methods for the recovery of protein species from wet mill grain process streams. Systems and methods of the present disclosure may be integrated with a wet mill grain process to separate out protein species that may limit efficiency of the grain process and produce one or more product streams comprising these separated protein species. A feed stream may be fractionated by at least two membranes into retentate and permeate streams. Removing larger proteins through the membrane fractionation may allow previously soluble prolamin products in the permeate stream(s) to precipitate. The recovered protein species may include prolamin, such as zein from a corn grain feed.

The present invention discloses a fried food coating with controlled release performance, a preparation method and an application thereof, and belongs to the technical field of fried foods. The preparation of the fried food coating with controlled release performance of the present invention includes: compounding a coagulant polysaccharide with carboxymethyl chitosan to obtain a composite polysaccharide solution; uniformly mixing alcohol-soluble protein with polyphenol to obtain an alcohol-soluble protein-polyphenol blended solution; then dripping the alcohol-soluble protein-polyphenol blended solution into a genipin solution to perform a self-assembly reaction to obtain an alcohol-soluble protein-polyphenol complex solution; then dripping the alcohol-soluble protein-polyphenol complex solution into the complex polysaccharide solution to perform an amino cross-linking reaction, thereby obtaining a fried food coating with controlled release performance. The coating has excellent antioxidant properties at room temperature and can extend the shelf life of fried foods; during high-temperature frying, the rapid release of active ingredients can be achieved, which can better maintain the safety and quality of fried foods.

The invention relates to the technical field of preparation of food preservative films, in particular to a zein-curcumin-resveratrol composite film and a preparation method and application thereof.The preparation method comprises the steps that zein and curcumin are dissolved in ethyl alcohol and then mixed with water, a resveratrol solution is dropwise added, stirring is conducted, and the zein-curcumin-resveratrol composite film is obtained; adding a chondroitin sulfate solution, homogenizing to form a nano suspension, finally blending with a gelatin solution, degassing, and drying to form the film. The composite membrane has good mechanical property, excellent water vapor and oxygen barrier property and remarkable hydrophobic property, and meanwhile, has lasting antioxidant and antibacterial functions by virtue of the slow release effect of curcumin and resveratrol. When used for packaging fresh food, especially aquatic products, the food packaging material can effectively inhibit lipid oxidation, protein degradation and microbial growth, and can significantly prolong the shelf life of food. All the components are natural, degradable, safe and environmentally friendly, and a new way is provided for high-performance active food packaging materials.

The present invention relates to a transgenic wheat event, which we have designated T258, that leads to reduced gliadin content, as well as cells, seeds and plants that comprise the event. The invention also relates to methods for detecting the presence of the T258 event, probes and primers for use in the method and methods of breeding with T258 to produce wheat with reduced gliadin content.

This invention discloses a gene that regulates the content of gliadin in rice grains. qPro7 This invention relates to the fields of genetic engineering and plant molecular breeding, and its applications. The amino acid sequence of the gene is shown in SEQ ID NO.1. Through genome-wide association analysis, this invention identified and cloned the gene on rice chromosome 7. qPro7 By constructing genes qPro7 Knockout vectors and self-promoted overexpression vectors were used to transform rice, and the results showed that knockout vectors... qPro7 The gene can significantly reduce the content of prolactin in rice grains, while overexpression qPro7 The gene significantly increases the content of prolamins in rice grains; this invention provides new genetic resources for the genetic improvement of rice quality, especially the regulation of protein content, and has important application value.

The utility model relates to an automatic device for extracting alcohol soluble protein from distillers' grains. The automatic device aims at efficiently extracting the alcohol soluble protein from the distillers' grains. The process comprises the following steps: receiving bran-containing vinasse from a vinasse pool, heating the vinasse into dry vinasse through a combustion pool, and conveying the dry vinasse to a screening device through a first conveyor belt; the screening device separates bran hulls from vinasse, the bran hulls are conveyed to the combustion pool through the second conveying belt, and the vinasse is stored in the storage bin. The vinasse in the storage bin is pulverized into powder by the pulverizing machine, the powder is conveyed to the stirring device and stirred in the alcohol solution, so that the alcohol-soluble protein is dissolved into the solution, and insoluble waste is also conveyed to the combustion pool through the second conveying belt. The alcohol solution containing the alcohol-soluble protein is stored in the pre-storage tank and then enters the drying machine, alcohol steam and the alcohol-soluble protein are separated out, and the alcohol-soluble protein is stored in the finished product storage tank. Alcohol steam is converted into alcohol liquid through the condensing device, the alcohol liquid is stored in the alcohol reflux tank, and the alcohol liquid flows back to the stirring device to be recycled after the concentration and the volume of the alcohol solution are adjusted through the storage tank. According to the device, all links are tightly matched, the full-automatic process from vinasse receiving, treatment and alcohol-soluble protein extraction to alcohol recycling is achieved, the alcohol-soluble protein extraction efficiency is improved, waste and alcohol are reasonably recycled, the cost is reduced, meanwhile, environmental pollution is reduced, and remarkable economic and environment-friendly benefits are achieved.

The invention belongs to the technical field of colloid and interface chemistry, and discloses a Pickering double emulsion and a preparation method thereof.The preparation method comprises the steps that enzymatic oxidation lignin is prepared on the basis of ball-milling activated lignin, alcohol-soluble protein-enzymatic oxidation lignin turbid liquid is prepared on the basis of alcohol-soluble protein ethanol water and the enzymatic oxidation lignin, and the Pickering double emulsion is obtained. A ternary composite nano dispersion liquid is prepared based on a carboxymethylated beta-glucan aqueous solution and a prolamin-enzymatic oxidation lignin suspension, an oil cake precipitate is obtained based on a curable oil phase and the ternary composite nano dispersion liquid, and a Janus particle dispersion liquid is prepared based on the oil cake precipitate and 2-morpholinoethanesulfonic acid. Obtaining an inner water phase / oil phase / outer water phase type Pickering double emulsion based on the Janus particle dispersion liquid, the inner water phase, the oil phase and the outer water phase; the method disclosed by the invention can show excellent stability and interface regulation and control capability.

The application provides a modification method of millet prolamin, uniformly mixing the millet prolamin with a solvent to obtain a millet prolamin solution; uniformly mixing a cationic surfactant with the millet prolamin solution to obtain a spinning solution; and electrospinning the spinning solution to obtain a millet prolamin nanofiber product. The cationic surfactant is added in the millet prolamin solution for modification, and the millet prolamin nanofiber product is successfully prepared, the mechanical strength and water stability of the nanofiber product are greatly improved, and the application of the millet prolamin is widened.

The invention provides a method for cultivating wheat suitable for processing cakes, which comprises the following steps of: hybridizing wheat materials deleted by different high-molecular-weight glutenin and wheat materials deleted by different alcohol-soluble proteins, carrying out molecular marker-assisted selection on offspring to obtain a series of new deletion bodies deleted by the high-molecular-weight glutenin and the alcohol-soluble proteins, and carrying out high-molecular-weight glutenin and alcohol-soluble protein co-deletion. Through cake processing quality determination, the gluten protein deletion body with excellent cake processing quality is screened out.

This application discloses the application and creation method of bread wheat with superior allelic variation at the Gli-A2p-null locus, belonging to the field of bio-breeding technology. The technical problem this application aims to solve is how to improve the quality of processed wheat grains. To this end, this application provides products made from raw materials including wheat, wherein the wheat does not contain the gliadin genes α / β3, α / β4, and α / β5. The wheat can be wheat GA2pN, with the CGMCC accession number CGMCC No. 45699. This application, through the determination of flour protein characteristics, found that GA2pN significantly improves many quality parameters, such as sedimentation index, wet gluten content, and gluten index, which are significantly superior to wild type. This application achieves the goal of significantly improving the quality of wheat dough and bread baking quality, and has important practical significance and application value, with broad application prospects.

This invention provides barley prolysin particles and their preparation method, as well as a method for preparing an oil-in-water emulsion, belonging to the field of food science and engineering technology. This invention employs proline endonuclease to enzymatically hydrolyze barley prolysin at a pH of 5.5-5.8, breaking it down into small-molecule barley prolysin polypeptides, thus reducing the size of the particles. Calcium ions in calcium chloride aqueous solution form coordination bonds with negatively charged groups such as carboxyl and hydroxyl groups on the barley prolysin polypeptide chain, regulating the charge distribution on the surface of the barley prolysin molecules and improving the dispersibility of the particles in water. Furthermore, the cycloalkenyl ether ketone active group in genipin molecules forms stable Schiff base covalent bonds with the free primary amino groups on the barley prolysin polypeptide chain, constructing a stable three-dimensional network structure and improving the thermal stability of the emulsion obtained from the barley prolysin particles.

The invention discloses a method for detecting gluten allergen alcohol-soluble protein based on multiple molecularly imprinted polymer enrichment. The method comprises the following steps: sample enzymolysis, preparation of multiple molecularly imprinted polymers, and enrichment and purification of multiple target molecules. Performing LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry) analysis, quantification and confirmation: respectively quantifying the three peptide fragments by using an external standard method until the concentration of any peptide fragment is gt; if the concentration is 1.0 mu g / kg, determining that the sample is gluten positive. According to the prepared multi-molecularly imprinted polymer, three peptide fragments serve as common templates, the multi-molecularly imprinted polymer capable of recognizing three structures at the same time is synthesized at a time, one-time synchronous enrichment of main gluten sensitizers is achieved, enzymolysis and multi-MISPE enrichment are converted into LC-MS / MS multi-target analysis, and the multi-molecular imprinted polymer has the advantages of being simple in preparation method, low in cost and good in application prospect. The excellent selective enrichment capacity of the multi-molecularly imprinted polymer is combined with the high specificity and high-sensitivity quantitative capacity of LC-MS / MS, and the problems of insufficient sensitivity and matrix interference of trace gluten protein detection in a complex matrix are solved.

The method comprises the following steps: extracting prolamin from vital gluten by using a 65% ethanol solution according to a solid-liquid ratio of 1: 30 (g: mL), wherein the stirring temperature is 30 DEG C and the stirring time is 3 hours; and carrying out rotary evaporation on the supernate to remove ethanol, refrigerating, precipitating and drying to obtain the alcohol-soluble protein. The method comprises the following steps: mixing amylose with a 20% NaCl solution according to a ratio of 1: 5 (g: mL), carrying out eutectic crystallization at 4 DEG C for 36 h, washing with water for 3-5 times, and drying the obtained precipitate at 50-60 DEG C to obtain the eutectic amylose. 8% and 2-10% of the flour are respectively added into the flour to prepare mixed powder, the addition amount of the yeast is 1-5% of the amount of the mixed powder, the addition amount of the table salt is 0.5-3% of the amount of the flour, the yeast and the table salt are sequentially added into the mixed powder, the mixed powder is dipped in warm water and stirred uniformly, and the mixed powder is prepared. And kneading dough, fermenting at normal temperature, kneading to form strips, and frying to obtain the fried dough twist with high crispness.