33 results about "Gliadin" patented technology

The application belongs to the field of SOD preparation, and discloses a preparation method and device of SOD resistant to pepsin degradation, which comprises the following steps: S1 powder preparation: dissolving SOD fermentation pure liquid in a buffer solution with pH of 5.2-7.8, adding protective agent and tea yellow in sequence, stirring, filtering, and placing in a freeze dryer to prepare freeze-dried powder; S2 drying; S3 tabletting; S4, coating liquid preparation: dissolving wheat gliadin and soybean protein in a mixed solution prepared from water and ethanol with a volume ratio of 1:1, wherein the mass ratio of the wheat gliadin and the soybean protein is 0.5-1:1, adding glycerol, stirring for 20 min by using a magnetic stirrer, and ultrasonicating for 40 s; S5, coating: placing the tablets in a coating machine, setting air inlet volume, air inlet temperature, pan rotating speed, atomization rate, fan face pressure, and mortar flow, until the coating weight increases to 3%-10% in mass fraction and the coating thickness is 20-50 μm, and then drying and cooling. The prepared SOD is not easy to be photolyzed and can resist gastric acid degradation to ensure the complete activity of the SOD.

The invention provides a wheat gliadin detection method, and belongs to the technical field of biology. The detection method comprises the following steps: mixing a sample to be detected with an ethanol solution to obtain a mixture; performing water bath on the mixture to obtain a mixed extract; centrifuging the mixed extract to obtain a supernatant; adding a reductive alkylation reagent into the supernate to obtain a reaction product; adding alkaline trypsin into the reaction product to carry out enzymolysis reaction to obtain an enzymolysis product; a first characteristic peptide fragment and a second characteristic peptide fragment as shown in SEQ ID NO: 1 in the sequence table are respectively used as markers, and the wheat gliadin is measured by the enzymolysis product through ultra-high performance liquid chromatography-tandem mass spectrometry by utilizing the markers. The method is simple in process and capable of efficiently detecting the wheat gliadin.

The invention discloses a preparation method of specific yolk immunoglobulin (IgY) for resisting gliadin. The preparation method comprises the following steps: immunizing and inoculating experimental laying hens, monitoring health behaviors, collecting eggs, and separating, extracting and purifying the IgY. The IgY prepared on the basis of the method can serve as a food base material to be added into low-gluten food (such as whole wheat bread and noodles), and the allergy risk caused by intake of a small amount of gluten is reduced through specific binding of the IgY and gliadin; patients are allowed to properly ingest gluten-containing food, diet diversity is improved, nutrient imbalance is avoided, and the life quality of the patients is improved. As the IgY has good thermal stability, the IgY purified by the method can still tolerate mild heating (such as baking and cooking) in food processing, and can be widely applied to various forms of products such as baked food, meal replacement powder, liquid beverage and the like; meanwhile, the acid-base stability of the antibody can ensure that the antibody keeps activity in a stomach environment after oral administration and finally reaches the intestinal tract to play a role, and the limitation that the conventional antibody is easy to digest and degrade after oral administration is broken through.

The invention belongs to the technical field of immunotherapy of allergic diseases, and provides a method for identifying dominant B cell epitopes and T cell epitopes in wheat omega-5 prolamin. According to the identification method provided by the invention, an immunoinformatics tool, a proliferation test, a degranulation test and the like are comprehensively applied, and the cell proliferation capacity, cell factor release, IgG / IgE binding capacity and degranulation capacity of T / B cell epitopes are evaluated. Results show that the T cell epitopes T1 and T2 sharing the same sequence are relatively strong in multiplication capacity, can up-regulate Th2 related cell factors, but do not have degranulation activity. Three B cell peptide fragments B3, B4 and B9 which share a common motif region QQXPQQQ (X = F, L) can promote IgG / IgE binding and degranulation capabilities. These findings would contribute to the development of epitope-based immunotherapies against wheat allergy individuals.

The invention provides a preparation method of a mixed biomass carbon coated silicon-based negative electrode material. Biomass carbon is mainly derived from prolamin, glutenin and high-activity yeast. Under the catalysis of high-activity yeast, prolamin and glutenin meet water to form a net-shaped structure, and the flexibility of the carbonized porous negative electrode material can be adjusted through different addition proportions, so that the flexible porous nitrogen-doped carbon-coated silicon-based negative electrode material can be obtained, the problem of volume expansion in the electrochemical process of a silicon negative electrode is relieved, and meanwhile, the conductivity is improved; the excellent electrochemical performance is shown. The porous silicon-based negative electrode material with controllable flexibility is obtained through drying and high-temperature annealing, the method is simple, effective and low in cost, and the prepared composite silicon-based negative electrode material is excellent in performance and suitable for large-scale production.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

The present invention relates to a method for producing 1B or 1D omega-5 gliadin-deleted wheat and wheat produced thereby, and, specifically, to a method for producing and selecting 1B or 1D omega-5 gliadin gene-deleted wheat by using radiation. The method for producing 1B or 1D omega-5 gliadin-deleted wheat, according to the present invention, enables the production of a radiation breeding-based non-GMO mutant wheat or wheat breeding parental line while retaining agronomic traits similar to those of a wild-type wheat variety, and thus wheat with reduced toxicity to wheat-dependent exercise-induced anaphylaxis (WDEIA) can be produced for use in the production of processed wheat foods and the like, or a parental line for breeding wheat varieties with reduced toxicity to WDEIA can be produced.

This invention discloses the application of corn protein coagulants or suspensions formed through liquid-liquid phase separation in bio-based adhesives or coatings. The preparation includes: dissolving corn gliadin or crude corn protein powder in a 70-90% v / v ethanol aqueous solution, removing impurities, adjusting the ethanol concentration to 40-60% v / v with water, and inducing phase separation to obtain coagulants; or directly adding corn gliadin to a 40-60% v / v ethanol aqueous solution and stirring to form coagulants. The resulting product is used as a bio-based adhesive for bonding sheet or paper-based materials, or as a coating to impart water / oil barrier properties to paper-based materials. This invention utilizes corn protein coagulants to achieve excellent adhesive performance and water resistance without crosslinking agents. The product is completely biodegradable, providing a green solution for replacing traditional plastic coatings and solving sheet bonding problems.

The application discloses a preparation method of antioxidant and pro-coagulation double-function sponge, and comprises the following steps: preparing an ethanol solution, adding corn alcohol-soluble protein to dissolve in the ethanol solution, adding deionized water with the same volume as the ethanol solution to perform reverse precipitation and ultrasonic dispersion, removing half volume of the solution in the dispersion liquid by rotary evaporation, adding glutaraldehyde aqueous solution to perform cross-linking, and obtaining cross-linking pro-coagulation nano-particle dispersion liquid; dissolving gelatin in hot water, stirring and dissolving at a low speed, adding glutaraldehyde, improving the rotating speed to perform chemical cross-linking, stopping foaming until uniform and stable foam is obtained, and obtaining foaming gelatin solution; pouring the obtained foaming gelatin solution into the cross-linking pro-coagulation nano-particle dispersion liquid, and obtaining antioxidant / pro-coagulation double-function sponge after freeze-drying; the volume fraction of the ethanol solution is 60-70%; the concentration of the corn alcohol-soluble protein solution is 10-50 mg / mL; and the volume ratio of the cross-linking pro-coagulation nano-particle dispersion liquid and the gelatin solution is 1:10-1:90.

The invention discloses a primer probe combination for detecting wheat and peanut allergens based on a dual Proofman-LMTIA technology and application of the primer probe combination, and belongs to the technical field of molecular detection. The primer probe combination comprises an XM-F1, an XM-B1, an XM-LB1, an HS-F2, an HS-B2, an HS-LB2, an XM-Probe1 and an HS-Probe2 which are as shown in SEQ ID NO. 1 to 8. The invention further discloses a kit for detecting the content of the XM-B1 in the kit. On the basis of a Proofman-LMTIA technology, specific primers and probes for wheat and peanut allergens are designed, a rapid detection method for wheat allergen gamma-gliadin and peanut allergen Arah 2.01 protein is established, and the method is used for detecting whether the wheat and peanut allergens are mixed in the links of production, processing and circulation of processed food. And technical support is provided for improving effective supervision of food supervision departments on allergen-containing products.

The present disclosure provides systems and methods for the recovery of protein species from wet mill grain process streams. Systems and methods of the present disclosure may be integrated with a wet mill grain process to separate out protein species that may limit efficiency of the grain process and produce one or more product streams comprising these separated protein species. A feed stream may be fractionated by at least two membranes into retentate and permeate streams. Removing larger proteins through the membrane fractionation may allow previously soluble prolamin products in the permeate stream(s) to precipitate. The recovered protein species may include prolamin, such as zein from a corn grain feed.

The invention relates to the technical field of preparation of food preservative films, in particular to a zein-curcumin-resveratrol composite film and a preparation method and application thereof.The preparation method comprises the steps that zein and curcumin are dissolved in ethyl alcohol and then mixed with water, a resveratrol solution is dropwise added, stirring is conducted, and the zein-curcumin-resveratrol composite film is obtained; adding a chondroitin sulfate solution, homogenizing to form a nano suspension, finally blending with a gelatin solution, degassing, and drying to form the film. The composite membrane has good mechanical property, excellent water vapor and oxygen barrier property and remarkable hydrophobic property, and meanwhile, has lasting antioxidant and antibacterial functions by virtue of the slow release effect of curcumin and resveratrol. When used for packaging fresh food, especially aquatic products, the food packaging material can effectively inhibit lipid oxidation, protein degradation and microbial growth, and can significantly prolong the shelf life of food. All the components are natural, degradable, safe and environmentally friendly, and a new way is provided for high-performance active food packaging materials.

This invention discloses a gene that regulates the content of gliadin in rice grains. qPro7 This invention relates to the fields of genetic engineering and plant molecular breeding, and its applications. The amino acid sequence of the gene is shown in SEQ ID NO.1. Through genome-wide association analysis, this invention identified and cloned the gene on rice chromosome 7. qPro7 By constructing genes qPro7 Knockout vectors and self-promoted overexpression vectors were used to transform rice, and the results showed that knockout vectors... qPro7 The gene can significantly reduce the content of prolactin in rice grains, while overexpression qPro7 The gene significantly increases the content of prolamins in rice grains; this invention provides new genetic resources for the genetic improvement of rice quality, especially the regulation of protein content, and has important application value.

The utility model relates to an automatic device for extracting alcohol soluble protein from distillers' grains. The automatic device aims at efficiently extracting the alcohol soluble protein from the distillers' grains. The process comprises the following steps: receiving bran-containing vinasse from a vinasse pool, heating the vinasse into dry vinasse through a combustion pool, and conveying the dry vinasse to a screening device through a first conveyor belt; the screening device separates bran hulls from vinasse, the bran hulls are conveyed to the combustion pool through the second conveying belt, and the vinasse is stored in the storage bin. The vinasse in the storage bin is pulverized into powder by the pulverizing machine, the powder is conveyed to the stirring device and stirred in the alcohol solution, so that the alcohol-soluble protein is dissolved into the solution, and insoluble waste is also conveyed to the combustion pool through the second conveying belt. The alcohol solution containing the alcohol-soluble protein is stored in the pre-storage tank and then enters the drying machine, alcohol steam and the alcohol-soluble protein are separated out, and the alcohol-soluble protein is stored in the finished product storage tank. Alcohol steam is converted into alcohol liquid through the condensing device, the alcohol liquid is stored in the alcohol reflux tank, and the alcohol liquid flows back to the stirring device to be recycled after the concentration and the volume of the alcohol solution are adjusted through the storage tank. According to the device, all links are tightly matched, the full-automatic process from vinasse receiving, treatment and alcohol-soluble protein extraction to alcohol recycling is achieved, the alcohol-soluble protein extraction efficiency is improved, waste and alcohol are reasonably recycled, the cost is reduced, meanwhile, environmental pollution is reduced, and remarkable economic and environment-friendly benefits are achieved.

The invention belongs to the technical field of colloid and interface chemistry, and discloses a Pickering double emulsion and a preparation method thereof.The preparation method comprises the steps that enzymatic oxidation lignin is prepared on the basis of ball-milling activated lignin, alcohol-soluble protein-enzymatic oxidation lignin turbid liquid is prepared on the basis of alcohol-soluble protein ethanol water and the enzymatic oxidation lignin, and the Pickering double emulsion is obtained. A ternary composite nano dispersion liquid is prepared based on a carboxymethylated beta-glucan aqueous solution and a prolamin-enzymatic oxidation lignin suspension, an oil cake precipitate is obtained based on a curable oil phase and the ternary composite nano dispersion liquid, and a Janus particle dispersion liquid is prepared based on the oil cake precipitate and 2-morpholinoethanesulfonic acid. Obtaining an inner water phase / oil phase / outer water phase type Pickering double emulsion based on the Janus particle dispersion liquid, the inner water phase, the oil phase and the outer water phase; the method disclosed by the invention can show excellent stability and interface regulation and control capability.

The application provides a modification method of millet prolamin, uniformly mixing the millet prolamin with a solvent to obtain a millet prolamin solution; uniformly mixing a cationic surfactant with the millet prolamin solution to obtain a spinning solution; and electrospinning the spinning solution to obtain a millet prolamin nanofiber product. The cationic surfactant is added in the millet prolamin solution for modification, and the millet prolamin nanofiber product is successfully prepared, the mechanical strength and water stability of the nanofiber product are greatly improved, and the application of the millet prolamin is widened.

The invention provides a method for cultivating wheat suitable for processing cakes, which comprises the following steps of: hybridizing wheat materials deleted by different high-molecular-weight glutenin and wheat materials deleted by different alcohol-soluble proteins, carrying out molecular marker-assisted selection on offspring to obtain a series of new deletion bodies deleted by the high-molecular-weight glutenin and the alcohol-soluble proteins, and carrying out high-molecular-weight glutenin and alcohol-soluble protein co-deletion. Through cake processing quality determination, the gluten protein deletion body with excellent cake processing quality is screened out.

This application discloses the application and creation method of bread wheat with superior allelic variation at the Gli-A2p-null locus, belonging to the field of bio-breeding technology. The technical problem this application aims to solve is how to improve the quality of processed wheat grains. To this end, this application provides products made from raw materials including wheat, wherein the wheat does not contain the gliadin genes α / β3, α / β4, and α / β5. The wheat can be wheat GA2pN, with the CGMCC accession number CGMCC No. 45699. This application, through the determination of flour protein characteristics, found that GA2pN significantly improves many quality parameters, such as sedimentation index, wet gluten content, and gluten index, which are significantly superior to wild type. This application achieves the goal of significantly improving the quality of wheat dough and bread baking quality, and has important practical significance and application value, with broad application prospects.

This invention provides barley prolysin particles and their preparation method, as well as a method for preparing an oil-in-water emulsion, belonging to the field of food science and engineering technology. This invention employs proline endonuclease to enzymatically hydrolyze barley prolysin at a pH of 5.5-5.8, breaking it down into small-molecule barley prolysin polypeptides, thus reducing the size of the particles. Calcium ions in calcium chloride aqueous solution form coordination bonds with negatively charged groups such as carboxyl and hydroxyl groups on the barley prolysin polypeptide chain, regulating the charge distribution on the surface of the barley prolysin molecules and improving the dispersibility of the particles in water. Furthermore, the cycloalkenyl ether ketone active group in genipin molecules forms stable Schiff base covalent bonds with the free primary amino groups on the barley prolysin polypeptide chain, constructing a stable three-dimensional network structure and improving the thermal stability of the emulsion obtained from the barley prolysin particles.

The present disclosure belongs to the technical field of functional ingredient extraction, and provides preparation and application of a distiller's grains arabinoxylan-sorghum prolamin complex. The preparation method comprises the following steps: drying and crushing distiller's grains, removing oil and starch, obtaining a suspension in an alkaline environment through infrared irradiation, stirring, shearing and centrifuging the suspension to obtain a supernatant, adjusting the supernatant to be acidic, centrifuging the supernatant again, alcohol precipitation, drying to obtain an extract, and coupling reaction at a proper temperature and humidity. The present disclosure also discloses the complex prepared by the above method, an emulsifier comprising the complex, and application of the emulsifier in the fields of food and cosmetics. The present disclosure adds infrared-assisted extraction, enhances dissolution and extraction of polysaccharides and sorghum prolamin, shortens extraction time and improves extraction rate; and further enhances emulsification through a dry heating method. The prepared complex has good water solubility, fast dissolution speed and strong emulsifying capacity, and can be used as a natural emulsifier in various scenarios.

The invention discloses an application of a Zm00001d029512 gene and a protein coded by the Zm00001d029512 gene in regulation and control of corn protein quality, and belongs to the technical field of plant genetic engineering. The CDS (Coding Sequence) of the Zm00001d029512 gene is as shown in SEQ ID NO. 1 (Sequence Identifier Number 1). According to the invention, the Zm00001d029512 gene is knocked out from corn, so that the accumulation proportion of prolamin in corn kernel endosperm can be effectively reduced, and meanwhile, the content of essential amino acids including lysine is remarkably increased. Experimental data prove that after the function of the gene is lost, the transcriptional level of various alcohol-soluble protein components is remarkably reduced, and the proportion of non-alcohol-soluble protein in grains is correspondingly increased, so that the composition of stored protein is fundamentally optimized, and the core nutritional defect of unbalanced amino acid composition in corn protein is directly solved. The invention provides a direct target for cultivating a new high-quality corn variety, and has important breeding application value.

The invention provides blood-glucose-reducing whole-grain rhizoma polygonati noodles and a preparation method thereof. The blood-glucose-reducing whole-grain rhizoma polygonati noodles comprise the following raw materials: 50-70 parts of whole-grain flour, 20-40 parts of wheat flour, 1-10 parts of blood-glucose-reducing paste and 1-10 parts of a rhizoma polygonati polysaccharide extract, the blood glucose reducing paste is prepared from the following raw materials: 28 to 32 parts of rhizoma polygonati, 13 to 17 parts of radix astragali seu hedysari, 8 to 12 parts of rhizoma dioscoreae, 8 to 10 parts of herba dendrobii, 10 to 14 parts of rhizoma polygonati odorati, 4 to 6 parts of radix puerariae, 8 to 10 parts of semen euryales and 3 to 5 parts of endothelium corneum gigeriae galli. According to the method, liquid nitrogen crushing and high-pressure homogenization are adopted, so that whole wheat crude fibers can be fully crushed, and the roughness caused by different sizes of the fibers is reduced; through low-temperature enzymolysis, sulfur groups of glutenin and gliadin are promoted to be oxidized into disulfide bonds to generate crosslinking, so that the toughness and the tenderness of dough are improved, the problem that soup is easy to muddy in the noodle cooking process is solved, and meanwhile, the natural breaking rate, the cooked breaking rate and the cooking loss rate of the noodles are reduced.

The invention belongs to the technical field of functional component extraction, and provides preparation and application of a vinasse araboxylan-sorghum prolamin compound. The preparation method comprises the following steps: drying and crushing the vinasse, and then carrying out degreasing and starch removal treatment; performing infrared irradiation in an alkaline environment to obtain turbid liquid; stirring, shearing and centrifuging the turbid liquid to obtain supernate, adjusting the supernate to be acidic, centrifuging to obtain supernate, performing alcohol precipitation, and drying to obtain an extract; and carrying out coupling reaction on the extract at proper temperature and humidity. The invention also discloses the compound prepared by the method, an emulsifier comprising the compound, and application of the emulsifier in the fields of food and cosmetics. Infrared-assisted extraction is added, so that dissolution and extraction of polysaccharide and sorghum prolamin are enhanced, the extraction time is shortened, and the extraction rate is increased; and the emulsifying property is further enhanced through a dry heating method. The prepared compound is good in water solubility, high in dissolving speed and high in emulsifying capacity, and can be used as a natural emulsifier in various scenes.

The invention relates to edible bio-ink, prolamin microspheres, cultured meat and a manufacturing method thereof. The edible bio-ink comprises prolamin microspheres loaded with cells and an ink solution containing gelatin and alginate, the edible bio-ink for three-dimensional printing of cultured meat is formed, and the particle size of the prolamin microspheres ranges from 150 m to 500 m.

The invention discloses a method for improving the starch digestion inhibition effect of protein, and belongs to the technical field of food processing. Highland barley starch, highland barley prolamin, highland barley glutelin and highland barley separation are selected as raw materials, and the embedding structure of protein in a natural grain structure on the surface of starch particles is simulated by using a spray drying technology. The preparation method comprises the following steps: dissolving protein in 0.02% NaOH solution, then adding highland barley starch, stirring for 30 minutes, then carrying out spray drying treatment, controlling the addition amount of the protein to be 10% of the total mass of the starch, setting the inlet temperature of a spray dryer to be 170 DEG C, setting the outlet temperature to be 80 DEG C, and controlling the feeding speed of a peristaltic pump to be 35 mL / min, the appearance of the prepared starch sample is similar to the spatial structure in highland barley grains, and the digestibility of the highland barley grains is reduced.

The application discloses a biological preparation method of a zein film and belongs to the technical field of natural high polymer material functional modification. In the application, a reducing agent L-cysteine is used to reduce disulfide bonds in zein, so that the molecular structure is loose, and more glutamine residues are exposed. On the basis, a transglutaminase catalytic acyl transfer reaction is used to introduce epsilon-polylysine rich in primary amino groups into the protein structure, so that the protein film with high physical and mechanical properties, water-soluble stability and high antibacterial activity is prepared, and the application effect of the protein film as food preservation material is improved.

This invention discloses a CMC zein@MOF aloe vera self-regulating antibacterial preservation film, its method, and its application, belonging to the technical field of food packaging, self-regulating, and antibacterial preservation films. This composite preservation film uses CMC and zein as the core matrix (mass ratio 4:1), and HKUST-1 nanoparticles (0.2% of the total mass of the core matrix) and aloe vera gel (6% of the total mass of the core matrix) as functional components. Through the synergistic effect of multiple components, it achieves triple preservation effects of physical barrier, active antibacterial activity, and ultraviolet shielding. Its preparation method includes HKUST-1 nanoparticle preparation, aloe vera gel extraction, core matrix solution preparation, film-forming solution preparation, and film drying. The process is simple, cost-controllable, and suitable for large-scale production. The composite film prepared by this invention has a tensile strength of 68.59 MPa, an antibacterial rate of nearly 100% against Escherichia coli and Staphylococcus aureus, a carbon dioxide / oxygen selective permeability of 8.04 g·kg·mmol / (m²·h), excellent biocompatibility, and a soil degradation rate of 54.38% after 60 days. It is mainly used for the preservation of high-moisture fruits such as strawberries, and can extend the shelf life of strawberries to more than 6 days at room temperature of 20-25℃. It effectively solves the problems of existing bio-based films having single function, poor preservation effect, and environmental non-environmental impact, and provides a green and efficient alternative to food preservation packaging, with broad prospects for industrial application.

The invention provides a highland barley-oat alcohol soluble protein fiber membrane as well as a preparation method and application thereof, and belongs to the technical field of food packaging. The composition comprises the following raw materials: highland barley alcohol-soluble protein, oat alcohol-soluble protein, glycerol and a phospholipid compound. The ratio of the total mass of the highland barley gliadin and the oat gliadin to the mass of the glycerol and the phospholipid compound is (20-30): (1.5-3.5): (1-3); the mass ratio of the highland barley alcohol-soluble protein to the oat alcohol-soluble protein is (6-8): (2-4); the feed also comprises a raw material bacteriostatic agent. Compared with a highland barley-corn / sorghum / millet gliadin fiber membrane, the highland barley-oat gliadin fiber membrane has the advantages that the mechanical property, the water blocking property and the antibacterial property are obviously improved, and the edible nanofiber membrane with excellent mechanical property, water blocking property and antibacterial property is successfully prepared; and a new technical path is provided for green and sustainable development of the food packaging industry.

The invention discloses application of a Zm00001d027461 gene in regulating and controlling the content of prolamin in plant grains, and belongs to the technical field of biology. The nucleotide sequence of the gene is as shown in SEQ ID NO.2, the CDS sequence of the gene is as shown in SEQ ID NO.3, and the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.1. The invention proves that the gene knockout mutation can lead to the obvious reduction of the expression quantity of gliadin genes such as 27kDa and 50kD in endosperm, the obvious reduction of the content of gliadin and the obvious increase of the content of lysine. Therefore, the Zm00001d027461 gene, the protein coded by the Zm00001d027461 gene and related biological materials provided by the invention can be used for regulating and controlling the content of prolamin in plant grains, and have great application and popularization values in the aspect of improving the nutritional quality of plants such as corn.

The application discloses a starch-based compound anti-aging gel and a preparation method thereof, and belongs to the technical field of food processing. The method is simple and fast, and has stronger product processing applicability and obvious anti-aging effect. The method has wide application prospects in the biomedical field and the food industry. The preparation method comprises the following steps: mixing starch, wheat alcohol-soluble protein and guar gum, and then adding water to prepare a starch protein and polysaccharide suspension. The suspension is heated to obtain a paste, and the paste is cooled to obtain the compound gel. The gel is a new type of material with wide application prospects, and has potential application values in the food industry, the biomedical field and other fields.