86 results about "Gliadin" patented technology

The invention relates to a double-layer preservative film for prolonging the shelf life of frozen beef and mutton after unfreezing and a preparation method of the double-layer preservative film. According to the preparation method of the angelica dahurica extract-gliadin nano-particles, the angelica dahurica extract-gliadin nano-particles can be used for preparing a double-layer preservative film, and the preservative film is a natural component and is environmentally friendly and safe. The preservative film is of a double-layer structure, the mechanical performance is enhanced through the double-layer film structure, and meanwhile the preservative film has excellent oxygen and water vapor barrier performance and an antibacterial effect. According to the preservative film disclosed by the invention, slow release of active ingredients is realized by utilizing the Pycnogenin-gliadin nanoparticles, so that the antioxidant and antibacterial effects are remarkably improved, and the quality guarantee period of meat is prolonged. In addition, the preservative film does not need to be in direct contact with food in practical application, potential safety hazards of the food are further reduced, the operation process is simplified, pollution is avoided, a user can conveniently use the preservative film, the preservative film has the advantages of efficient preservation and environmental protection, and the preservative film has good application prospects and value.

The invention belongs to the technical field of cereal protein processing, and particularly relates to wheat gliadin with good color and luster and glutenin with high solubility as well as a preparation method and application of the wheat gliadin and the glutenin. According to the method, a cold water solubility control coupling one-step freezing purification technology is broken through, the concentration of ethanol and the temperature of the solution are controlled through cold water, the dissolving state and aggregation behavior of wheat gliadin are synchronously regulated and controlled, then freezing treatment and a one-step method are coupled to achieve controllable protein aggregation, precipitation and freezing, and the purposes of separation and purification are achieved. The technology is green, efficient, simple, convenient and energy-saving, the obtained alcohol-soluble protein is good in color and luster, and industrial production is easy to realize. Furthermore, a membrane separation coupling high-pressure microjet technology is broken through, and on the basis of a technology for obtaining a glutenin concentrated solution through membrane separation, the protein concentrated solution is directly treated by adopting an alkaline condition synchronous high-pressure microjet circulating modification method, so that the structural characteristics such as the scale and the surface hydrophobicity of the target protein and the aggregation behavior are further regulated and controlled; the glutenin is endowed with better solubility.

The invention discloses a high-protein-content enzymolysis corn protein powder processing method which comprises the following steps: S1, pretreatment: pretreating corn protein powder to obtain low-starch-content wet corn protein powder; s2, enzymolysis: starch in the corn protein powder is removed, the enzymolysis comprises I-segment enzymolysis and II-segment enzymolysis, one compound protease is firstly used for I-segment enzymolysis, and then the other compound protease is used for II-segment enzymolysis; and S3, spray drying: finally, spray drying is performed to generate the sectional type enzymolysis processing corn protein powder of the product, on one hand, the starch content in the corn protein powder is reduced, and the dosage limitation of the corn protein powder in aquatic feed is relieved; on the other hand, the corn protein powder is modified and added, most of prolamin and glutelin in the raw materials are changed into water-soluble protein and small peptide, and the animal utilization rate is greatly increased; in addition, the product can partially replace fish meal, so that feed resources are expanded, dependence on the fish meal is reduced, and the cost of an aquatic feed formula is also reduced.

The invention belongs to the technical field of wheat gliadin drug-loaded nanoparticle processing, and particularly relates to lutein-loaded composite modified wheat gliadin nanoparticles as well as a preparation and application thereof. The composite modified wheat gliadin nanoparticles are modified by combining enzymolysis with dextran grafting, and the dextran grafting adopts a low-temperature-high-temperature circulation method, so that the grafting degree is remarkably improved, and the browning degree is relatively low. According to the lutein-loaded composite modified wheat gliadin nanoparticles, pH folding is combined with an ultrasonic method, so that the encapsulation efficiency of lutein is remarkably improved. The obtained nanoparticles can significantly improve the illumination and thermal stability of the nanoparticles and the stability in the gastrointestinal tract environment, are suitable for improving the oral utilization rate of xanthophyll, and are used for preparing oral drugs, oral health-care foods and the like.

The stability of polyphenol is improved based on steric hindrance of the nanoparticles, the slow-release characteristic of the polyphenol in the gastrointestinal digestion process is improved, and the problems that traditional starch is easy to digest and hydrolyze, and the like are solved. And the problems of complex preparation steps or unstable polyphenol inhibition digestion caused by resistant starch and slowly digestible starch are solved. And the activity of amylase can be effectively inhibited by continuously releasing polyphenol, so that the digestibility of starch is reduced. And secondly, the polyphenol used in the invention has biological activity, and the prolamin nanoparticles show excellent mucous membrane adhesiveness in gastrointestinal digestion and absorption processes, so that the bioaccessibility of the encapsulated bioactive compound is increased, and the preparation method has higher application value and is suitable for wide popularization and application.

A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.

The invention discloses rice LOC_Os01g68930 The application of genes in regulating the protein content in rice grains relates to the field of genetic engineering technology. LOC_Os01g68930 The CDS sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO. 2. The present invention obtains OE‑LOC_Os01g68930 Overexpression plants. Experiments have shown that: PLACE_ Os01g68930 The total content of glutenin and alcohol-soluble proteins in the seeds of overexpressing transgenic rice reached 7.88% to 7.97%. In comparison, the protein content of wild-type rice grains was 6.97%, and the protein content of transgenic rice grains increased by 13.1%-14.3%. LOC_Os01g68930 It has been confirmed that this gene can effectively regulate the protein content of rice grains, laying a solid foundation for the subsequent breeding of high-protein rice varieties.

The invention belongs to the technical field of plant genetic engineering, and discloses an application of a corn gene ZmMADS8 in control of protein quality, a protein encoded by the ZmMADS8 is shown in SEQ ID NO.2, and the gene is located in the ninth chromosome of corn and used for controlling the content of zein and / or lysine. The gene is knocked out by using a CRISPR / Cas9 technology, gene expression is inhibited, and the content of prolamin and / or lysine in corn can be increased.

The invention relates to a cationic tannin and prolamin composite nano system and a preparation method and application thereof.The cationic tannin and prolamin composite nano system is prepared through the steps that cationic tannin and prolamin are dissolved in an alcoholic solution through an anti-solvent dispersion method, then the alcoholic solution is dropwise added and dispersed in water, and the cationic tannin and prolamin composite nano system is obtained. Nano-particles are formed through self-assembly under the interaction of non-covalent acting force between cationic tannin and prolamin in an aqueous solution, and the composite nano-system is applied to water treatment. Compared with the prior art, the water treatment agent is good in storage stability and has excellent performance of removing antibiotic pollution in a water body.

The invention belongs to the field of food engineering and the field of biological macromolecular materials, and particularly relates to a preparation method and application of oleogel based on protein fibers. The oleogel is prepared from the prolamin from barley and different protein fiber and xanthan gum compounds, the obtained oleogel has high oil holding capacity, high storage modulus, better thixotropic resilience and good temperature and thermodynamic stability, and different types of oleogels can be suitable for different processing scenes, so that the oleogel can be applied to different processing fields. The method can be widely applied to the fields of foods, medicines and cosmetics.

The invention provides a construction method and an application method of a wheat yeast two-hybrid cDNA library, and belongs to the technical field of biology. The invention provides a construction method of a yeast two-hybrid cDNA library, which comprises the following steps: extracting total RNA, separating mRNA, converting into cDNA, transferring into escherichia coli competent cells through a series of reactions to obtain a cDNA primary library bacterial liquid, separating primary library plasmids, and obtaining a yeast two-hybrid cDNA library bacterial liquid through an LR recombination reaction and conversion of the escherichia coli competent cells. A high-quality wheat variety Zheng wheat 158 is taken as a research material, wheat gliadin genes Tagli-gamma-1 and Tagli-gamma-11 are cloned, a corresponding bait vector is constructed, the interaction protein is screened from a cDNA library of the Zheng wheat 158 by utilizing a yeast two-hybrid technology, and a foundation is laid for carrying out interaction protein screening of genes related to wheat quality and analyzing a regulation mechanism of the interaction protein in a later period.

The present invention relates to the field of gene detection technology, and in particular to a method for synchronously detecting soybean protein and wheat protein allergen genes by droplet digital PCR. The method comprises: extracting DNA of the sample to be tested, performing PCR amplification using a primer and probe combination, and judging whether the sample to be tested contains soybean protein allergens or wheat protein allergens according to the amplification result. The primer and probe combination comprises a primer pair shown in SEQ ID NO.1‑2, 4‑5 and a probe shown in SEQ ID NO.3, 6. The present invention designs specific primers and probes for specific targets of soybean allergen protein gene β‑conglycinin and wheat allergen protein gene α / β‑gliadin. The two sets of primers and probes can be used in an amplification system, and the soybean protein and wheat protein allergen genes in the sample can be detected by one reaction, which is of great significance in the allergen detection system.

The application belongs to the field of SOD preparation, and discloses a preparation method and device of SOD resistant to pepsin degradation, which comprises the following steps: S1 powder preparation: dissolving SOD fermentation pure liquid in a buffer solution with pH of 5.2-7.8, adding protective agent and tea yellow in sequence, stirring, filtering, and placing in a freeze dryer to prepare freeze-dried powder; S2 drying; S3 tabletting; S4, coating liquid preparation: dissolving wheat gliadin and soybean protein in a mixed solution prepared from water and ethanol with a volume ratio of 1:1, wherein the mass ratio of the wheat gliadin and the soybean protein is 0.5-1:1, adding glycerol, stirring for 20 min by using a magnetic stirrer, and ultrasonicating for 40 s; S5, coating: placing the tablets in a coating machine, setting air inlet volume, air inlet temperature, pan rotating speed, atomization rate, fan face pressure, and mortar flow, until the coating weight increases to 3%-10% in mass fraction and the coating thickness is 20-50 μm, and then drying and cooling. The prepared SOD is not easy to be photolyzed and can resist gastric acid degradation to ensure the complete activity of the SOD.

The method comprises the following steps: dissolving table salt or crude salt in water to prepare 5-20% saline water, adding amylopectin accounting for 20-30% of the saline water, standing and culturing for 12-72 hours at 4-30 DEG C, centrifuging, washing with water, precipitating, and drying to obtain the salt eutectic wheat amylopectin. The preparation process of the alkali-soluble glutenin comprises the following steps: mixing vital gluten and 65% ethanol according to the mass ratio of 1: 20, and stirring and extracting in a water bath kettle at 35 DEG C for 3 hours to dissolve the alcohol-soluble protein in the vital gluten in the ethanol; and centrifuging the extracted solution to obtain the precipitate, namely the glutenin aggregate. Dissolving the glutenin aggregate for 120 minutes at the temperature of 30 DEG C by using 0.1% sodium hydroxide, and standing for 12 hours at the temperature of 50 DEG C to generate precipitates; and dialyzing the precipitate by using a dialysis bag, and drying to obtain the alkali-soluble glutenin. And mixing the eutectic wheat amylopectin, alkali-soluble glutenin accounting for 10% of the starch amount and drinking water accounting for 30% of the starch amount, stirring at 25-35 DEG C for 10-60 minutes, drying and crushing to obtain the flour gluten strengthening agent.

The invention provides a wheat gliadin detection method, and belongs to the technical field of biology. The detection method comprises the following steps: mixing a sample to be detected with an ethanol solution to obtain a mixture; performing water bath on the mixture to obtain a mixed extract; centrifuging the mixed extract to obtain a supernatant; adding a reductive alkylation reagent into the supernate to obtain a reaction product; adding alkaline trypsin into the reaction product to carry out enzymolysis reaction to obtain an enzymolysis product; a first characteristic peptide fragment and a second characteristic peptide fragment as shown in SEQ ID NO: 1 in the sequence table are respectively used as markers, and the wheat gliadin is measured by the enzymolysis product through ultra-high performance liquid chromatography-tandem mass spectrometry by utilizing the markers. The method is simple in process and capable of efficiently detecting the wheat gliadin.

A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.

The invention discloses application of a ZmEREB93 gene knockout biological material in cultivation of high-yield and high-quality corn, and relates to the technical field of biology. The ZmEREB93 gene knockout strain is constructed, homozygous seeds of the ZmEREB93 gene knockout strain are subjected to seed test analysis, and it is found that corn kernels of the knockout strain are remarkably superior to a wild type in the aspects of the grain length, the grain width, the hundred-grain weight and the single plant yield. In the early, middle and late periods of grain filling, the corn grains of the knockout strain are obviously superior to a wild type control in shape and endosperm structure. Further determination of starch content and protein content shows that the starch content of seeds of the knockout strain is significantly higher than that of a wild type control group, the alcohol-soluble protein content is also significantly increased, and the total protein content is significantly higher than that of a control group. Therefore, the ZmEREB93 gene has an important application value in cultivation of a high-yield and high-quality new corn variety.

The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in regulating and controlling the protein content of plant seeds, and belongs to the technical field of molecular biology. The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in at least one of regulation and control of protein content of plant seeds, creation of transgenic plants with dominant protein content and breeding of plant varieties with dominant protein content. The result of the embodiment of the invention shows that the seed protein content of a TaMYB46L-5A overexpression plant is obviously reduced, the seed protein content of a TaMYB46L-5A mutant plant is increased, and the contents of prolamin and glutenin are obviously increased. Therefore, the TaMYB46L-5A can negatively regulate and control the protein content of the plant seeds, and plays an important role in plant breeding.

The invention provides high-purity instant honey powder and a preparation method thereof. The preparation of the high-purity instant honey powder is realized by adding gliadin and xanthan gum and combining a low-temperature plasma sterilization technology and spray drying. The added gliadin and xanthan gum can reduce the combination degree of honey and water and reduce the water absorption capacity; the gliadin and the xanthan gum form a gel network structure, so that the spray-dried honey powder has higher dispersity and is not easy to cake, and the instant solubility of the honey powder is improved; meanwhile, cane sugar and gliadin in the honey are combined through hydrogen bonds, the glass transition temperature is increased, the water content is reduced, the honey powder is not prone to moisture absorption and caking, and the market applicability is wider.

The invention discloses a composite nanofiber membrane containing wheat gliadin and a preparation method of the composite nanofiber membrane. The composite nanofiber membrane comprises an inner layer and an outer layer, wherein the inner layer comprises wheat gliadin, gelatin, eugenol and a plasticizer; the outer layer is prepared from wheat gliadin, carboxymethyl chitosan, layered silicate nanosheets, a cross-linking agent and a dispersing agent. The wheat gliadin, the natural polymer and the nano material are used for constructing a double-layer structure with complementary functions through an electrospinning technology, so that excellent antibacterial property, barrier property and mechanical property are realized, and the shelf life of meat food can be effectively prolonged; a full-bio-based material is adopted, so that the environmental problem that a traditional plastic packaging material is difficult to degrade is solved; the preparation process is simple and convenient, controllable in parameters and suitable for large-scale production; meanwhile, multiple requirements of food safety, fresh-keeping effect and environment friendliness are met, and a novel efficient and green solution is provided for the field of meat food packaging.

The invention discloses a preparation method of specific yolk immunoglobulin (IgY) for resisting gliadin. The preparation method comprises the following steps: immunizing and inoculating experimental laying hens, monitoring health behaviors, collecting eggs, and separating, extracting and purifying the IgY. The IgY prepared on the basis of the method can serve as a food base material to be added into low-gluten food (such as whole wheat bread and noodles), and the allergy risk caused by intake of a small amount of gluten is reduced through specific binding of the IgY and gliadin; patients are allowed to properly ingest gluten-containing food, diet diversity is improved, nutrient imbalance is avoided, and the life quality of the patients is improved. As the IgY has good thermal stability, the IgY purified by the method can still tolerate mild heating (such as baking and cooking) in food processing, and can be widely applied to various forms of products such as baked food, meal replacement powder, liquid beverage and the like; meanwhile, the acid-base stability of the antibody can ensure that the antibody keeps activity in a stomach environment after oral administration and finally reaches the intestinal tract to play a role, and the limitation that the conventional antibody is easy to digest and degrade after oral administration is broken through.

The invention belongs to the technical field of immunotherapy of allergic diseases, and provides a method for identifying dominant B cell epitopes and T cell epitopes in wheat omega-5 prolamin. According to the identification method provided by the invention, an immunoinformatics tool, a proliferation test, a degranulation test and the like are comprehensively applied, and the cell proliferation capacity, cell factor release, IgG / IgE binding capacity and degranulation capacity of T / B cell epitopes are evaluated. Results show that the T cell epitopes T1 and T2 sharing the same sequence are relatively strong in multiplication capacity, can up-regulate Th2 related cell factors, but do not have degranulation activity. Three B cell peptide fragments B3, B4 and B9 which share a common motif region QQXPQQQ (X = F, L) can promote IgG / IgE binding and degranulation capabilities. These findings would contribute to the development of epitope-based immunotherapies against wheat allergy individuals.

The invention provides a preparation method of a mixed biomass carbon coated silicon-based negative electrode material. Biomass carbon is mainly derived from prolamin, glutenin and high-activity yeast. Under the catalysis of high-activity yeast, prolamin and glutenin meet water to form a net-shaped structure, and the flexibility of the carbonized porous negative electrode material can be adjusted through different addition proportions, so that the flexible porous nitrogen-doped carbon-coated silicon-based negative electrode material can be obtained, the problem of volume expansion in the electrochemical process of a silicon negative electrode is relieved, and meanwhile, the conductivity is improved; the excellent electrochemical performance is shown. The porous silicon-based negative electrode material with controllable flexibility is obtained through drying and high-temperature annealing, the method is simple, effective and low in cost, and the prepared composite silicon-based negative electrode material is excellent in performance and suitable for large-scale production.

The invention belongs to the field of SOD preparation, and discloses a pepsin degradation resistant SOD preparation method and device, the method comprises the following steps: S1, powder preparation: dissolving SOD fermentation pure liquid in a buffer solution with the pH value of 5.2-7.8, sequentially adding a protective agent and theaflavin, stirring, filtering, and putting into a freeze dryer to prepare freeze-dried powder; s2, drying; s3, tabletting; s4, preparing a coating solution, namely dissolving wheat gliadin and soybean protein in a mixed solution prepared from water and ethanol in a volume ratio of 1: 1, adding a plasticizer, stirring for 20 minutes by using a magnetic stirrer, and performing ultrasonic treatment for 40 seconds, wherein the mass ratio of the wheat gliadin to the soybean protein is (0.5-1): 1; and S5, coating: placing the tablets in a coating machine, setting the air inlet rate, the air inlet temperature, the pot rotating speed, the atomization rate, the sector pressure and the guniting flow until the weight of the coating is increased to 3-10% by mass and the thickness of the coating is 20-50 microns, and drying and cooling. The method has the beneficial effects that the prepared SOD is not easy to be photolyzed, and can resist gastric acid degradation, so that the complete activity of the SOD is ensured.

The invention discloses a gluten specific alpaca polyclonal antibody and a double antibody-streptavidin enzyme-linked immunosorbent assay (ELISA) bran quality inspection test kit thereof. The detection kit provided by the invention takes an alpaca polyclonal antibody pAb as a capture antibody and biotinylated pAb as a detection antibody to carry out ELISA detection on gluten in a food matrix, and a novel double antibody-streptavidin ELISA detection method is constructed based on the detection kit. The detection method has good sensitivity and specificity, the EC50 value of alcohol-soluble protein is 51.21 ng / mL, the detection limit is as low as 0.07 ng / mL, the detection limit of the method is 4000 times lower than that of an existing R5 antibody method, and the sensitivity is better; when the alpaca polyclonal antibody pAb prepared by the invention is used for detection, the detection cost is reduced, reliable technical support is provided for gluten allergen detection, and the alpaca polyclonal antibody pAb is suitable for clinical and scientific research application of immunoassay kits, immune test strips and the like.

The preparation method comprises the following steps: mixing vital gluten and 65% ethanol according to a mass ratio of 1: 30, and stirring and extracting in a water bath kettle at 35 DEG C for 3 hours to dissolve alcohol-soluble protein in the vital gluten in the ethanol; after standing, centrifuging for 5 minutes by using a centrifugal machine under the condition of 3,500 r / min, taking the precipitate, repeating the steps for dissolving, centrifuging the solution for 5 minutes, and taking the precipitate, namely the glutenin aggregate. Dissolving the glutenin aggregate at 30 DEG C for 120 minutes by using 0.1% sodium hydroxide alkali liquor, standing at 50 DEG C for 12 hours, and regulating the pH value to be neutral to generate precipitate; and centrifuging for 5 minutes to obtain a precipitate which is alkali-soluble glutenin. Amylose and alkali-soluble glutenin are mixed according to the mass ratio of 1: 8-1: 10, drinking water accounting for 3%-5% of the starch amount is added, mixing is conducted for 30-50 min at the temperature of 30-35 DEG C, and the disulfide bond content of the alkali-soluble glutenin is reduced by 80% or above.

The present invention belongs to the technical field of microbial engineering, and particularly relates to a Bacillus subtilis for enhancing the available protein in alcoholic corn distillers grains and the preparation and application of its composite bacterium agent. The present invention provides a Bacillus subtilis with a preservation number of CGMCC No. 32291. The Bacillus subtilis of the present invention can secrete protease and cellulase, degrade macromolecular proteins in alcoholic corn distillers grains into small molecular weight proteins during fermentation, and reduce the content of prolamin. The present invention also provides a composite bacterium agent, which includes Lactobacillus plantarum and the Bacillus subtilis described in the above technical solution. The composite bacterium agent of the present invention can increase the viable bacteria count and the content of corresponding beneficial metabolites in fermented alcoholic corn distillers grains, enrich the nutrition of alcoholic corn distillers grains, and improve the utilization rate of alcoholic corn distillers grains.

The invention provides a preparation method of uric acid reducing microsphere particles containing a dendrobium huoshanense leaf extract, and belongs to the technical field of uric acid reducing compositions.The specific method includes the steps that fresh dendrobium huoshanense leaves are subjected to alcohol extraction to obtain a dendrobium huoshanense leaf ethanol extracting solution, and then the dendrobium huoshanense leaf ethanol extracting solution is purified through an AB-8 resin chromatographic column to obtain the uric acid reducing microsphere particles containing the dendrobium huoshanense leaf extract. The brand-new natural dendrobium huoshanense leaf extract microsphere particle for reducing uric acid is obtained by combining a corn polypeptide prolamin mixed solution formed by mixing a corn prolamin solution and a corn polypeptide solution, has the effect of remarkably reducing uric acid of mice, and has the advantages that the stability of effective components is kept after the particle is stored for 1-2 months, and the bioavailability of the particle is improved. The uric acid value of a hyperuricemia mouse can be obviously reduced, and the economic value is very good.

The present invention relates to a kind of alcohol-soluble protein-milk protein composite hollow particles and a preparation method and application thereof, belonging to the field of food technology. In the alcohol-soluble protein-milk protein composite hollow particles of the present invention, alcohol-soluble protein and milk protein are covalently cross-linked to form two kinds of protein mixed shells. The present invention selects alcohol-soluble protein and milk protein as shell materials, and performs alkaline heating treatment on the ethanol solutions of the two, so that covalent action occurs between alcohol-soluble protein and milk protein to form a mixed shell structure, which on the one hand regulates the hydrophilicity and hydrophobicity of the shell, and on the other hand is conducive to the stabilization of the shell formation; improves the water dispersibility and stability of alcohol-soluble protein-milk protein composite hollow particles. On this basis, a hydrophobic functional factor is added to the composite solution of alcohol-soluble protein and milk protein after alkaline heating treatment, and alcohol-soluble protein-milk protein composite hollow particles loaded with hydrophobic functional factors are prepared, so as to achieve efficient embedding of the hydrophobic functional factors.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.