2results about How to "Promote development and utilization" patented technology

The application belongs to the field of molecular biology identification, and particularly relates to a specific SNP site combination for identifying Duntou pig breeds and application. The specific SNP site combination for identifying Duntou pig breeds comprises 40 SNP sites, and the physical position is based on the reference genome of pigs Sscrofa11.1 The sequence comparison is determined. The specific SNP site combination is screened based on the method of whole genome association analysis combined with selection signal analysis, so as to ensure the accuracy of site selection. The specific SNP site combination provided by the application can rapidly realize the accurate identification of Duntou pig breeds at the gene level, and has significant application value in the accurate protection of Hainan local pig genetic resources and breed utilization.

The application discloses a method for improving gas production performance of straw and pig manure mixture dry biogas fermentation based on multi-factor cooperation, which comprises the following steps: mixing pig manure and straw at a ratio of 1:1 as the best feed substrate according to total solids, adopting semi-continuous dry biogas fermentation process, feeding the substrate into a biogas fermentation reactor, inoculating anaerobic sludge domesticated by pig manure, controlling the total solid concentration of the feed substrate to be 20%-30%, controlling the hydraulic retention time to be 25-45 days, feeding air into the fermentation process for air stirring, controlling the stirring intensity to be 33.3-66.7 mL / (L.min), and stirring once a day for 1 minute each time. The straw and pig manure mixture dry biogas fermentation gas production performance can be effectively improved through the synergistic effect of the optimized raw material ratio, the total solid concentration of the feed, the hydraulic retention time and the air stirring intensity.