32results about How to "Promote development and utilization" patented technology

The invention discloses a larix olgensis SNP (Single Nucleotide Polymorphism) molecular marker, a whole-genome liquid phase chip prepared from the molecular marker and application of the whole-genome liquid phase chip. The larix olgensis SNP molecular marker comprises at least one of 61 and 680 SNP molecular markers, and the physical positions of the 61 and 680 SNP molecular markers are determined by performing sequence alignment based on a Japanese larix reference genome GCA027924585.1; the larix olgensis SNP molecular marker disclosed by the invention can be used for specifically identifying larix olgensis trees, and sites are used for preparing a larix olgensis tree species whole genome liquid phase chip; low-cost genetic typing, genetic diversity evaluation of larix olgensis, identification of germplasm resources and genetic relationships, molecular design breeding, genetic map construction and functional gene positioning, whole genome association analysis and intelligent breeding can be achieved, and the method has high application value in multiple fields of larix olgensis breeding.

The invention discloses biocontrol marine source penicillium and application thereof, and relates to the technical field of microorganisms. The biocontrol marine source penicillium is named as Penicillium sp.DSF059 and is preserved in the China Center for Type Culture Collection, and the preservation number of the biocontrol marine source penicillium is CCTCC NO: M 20232310. The invention discloses biocontrol marine-source penicillium. The biocontrol marine-source penicillium has effects on rubber fusarium wilt pathogenic bacteria, coconut gray leaf spot pathogenic bacteria, wheat scab pathogenic bacteria, cucumber phytophthora root rot pathogenic bacteria, avocado phytophthora root rot pathogenic bacteria, rubber anthracnose pathogenic bacteria, mango anthracnose pathogenic bacteria, sisal zebra stripe pathogenic bacteria, rubber red root pathogenic bacteria, bacillus subtilis and bacillus subtilis. The invention provides a new direction for the prevention and treatment of the tropical crop pathogenic bacteria, and has a very good development and application prospect, and the tropical crop pathogenic bacteria such as the pathogenic bacteria of the rubber brown root disease, the coffee anthracnose pathogenic bacteria, the stylosanthes guianensis anthracnose pathogenic bacteria and the banana anthracnose pathogenic bacteria can be prevented and treated.

The invention provides a feline animal bifidobacterium, a metagen, a pharmaceutical composition and application, the feline animal bifidobacterium is named as animal bifidobacterium GYJC-001, the strain is preserved in the China Center for Type Culture Collection on July 22, 2024, and the preservation number is CCTCC NO: M 20241621. The invention further provides a preparation method of the feline animal bifidobacterium, the metagen, the pharmaceutical composition and the application of the feline animal bifidobacterium, the metagen, the pharmaceutical composition and the application of the feline animal bifidobacterium, the metagen, the pharmaceutical composition and the application of the feline animal bifidobacterium. The bifidobacterium animalis GYJC-001 disclosed by the invention has the advantages that the acid resistance and cholate resistance are high, the intestinal colonization ability is strong, the probiotic effect is better, the toxic and side effects are avoided, and a certain theoretical basis is provided for the development of the bifidobacterium animalis GYJC-001.

The invention discloses an underground enhanced heat exchange method for a closed heat removal system, and belongs to the field of geothermal energy development and utilization. A vertical well is drilled according to the depth and thickness of a heat reservoir, a casing pipe is put down and well cementation is carried out, a central heat preservation pipe is arranged in the casing pipe, and an annular space between the casing pipe and the central heat preservation pipe forms a vertical well annulus; casing lateral windowing is carried out on the vertical well section corresponding to the thermal reservoir, a plurality of branch well holes are drilled, or volume fracturing is carried out to form micro cracks; filling a high-thermal-conductivity material in the multilateral well hole / microcrack, transforming the thermal reservoir and supporting the transformed space to form a high-thermal-conductivity area in the thermal reservoir, and plugging the lateral multilateral well hole to complete the transformation of the thermal reservoir; a heat exchange working medium is injected through a high-pressure pump through a vertical well annulus, is pumped out of the central heat preservation pipe after being subjected to closed type sufficient heat exchange with the transformed heat storage layer, and geothermal energy utilization is achieved through the heat exchanger. The defects of a traditional closed heat removal system are remarkably overcome, the heat removal temperature is increased, the heat removal service life is prolonged, and development and utilization of medium-deep geothermal energy are accelerated.

The invention discloses a glycocholic acid sustained release preparation and a production process thereof. The glycocholic acid sustained-release preparation comprises glycocholic acid sustained-release pellets, and the preparation method of the glycocholic acid sustained-release preparation comprises the following steps: firstly, preparing glycocholic acid and auxiliary materials into a drug-containing pellet core according to a formula, then coating the drug-containing pellet core with a sustained-release material to obtain the sustained-release pellets, and finally, filling the sustained-release pellets into capsules to obtain the glycocholic acid sustained-release preparation. The preparation method comprises the following steps: carrying out wet granulation-extrusion rolling to obtain wet pills, drying, and coating with a fluidized bed to obtain the glycocholic acid sustained-release pellets. The quality of the drug-loaded pellet is controlled according to indexes such as roundness, bulk density, yield and the like, the drug release effect of the sustained-release pellet is evaluated according to the dissolution rate, and the release mechanism and the initial stability of the glycocholic acid sustained-release pellet are investigated. The invention has the advantages of favorable sustained-release effect, reduced medication frequency, simple and feasible preparation process, favorable reproducibility and favorable drug stability, is suitable for industrial production, aims at supporting the research and development of the compound sustained-release drug, and has obvious social and economic benefits.

The application relates to a method for hydrothermal acid-controlled degradation of mulberry leaf polysaccharide and antioxidant application thereof, and belongs to the technical field of extraction of active ingredients in natural products. The method comprises the following steps: pretreating raw materials, hydrothermally reacting and acid-catalytically treating mulberry leaves, and alcohol-sedimenting, separating and purifying mulberry leaf polysaccharide, so as to obtain mulberry leaf polysaccharide, finally, the antioxidant application of the mulberry leaf polysaccharide is explored, and a theoretical basis is provided for development of mulberry leaf resources. The method can not only obtain mulberry leaf polysaccharide, but also obtain high-value natural active ingredients such as rutin, astragalin, scopoletin and isoquercitrin in the mulberry leaves, is beneficial to development and utilization of the mulberry leaf resources, meets the needs of people's health, and has practical significance and economic value.

The liver-protecting health-care tea comprises 20 parts of Pu'er tea, 10 parts of centella asiatica leaves, 10 parts of nutgall, 20 parts of dried longan, 10 parts of chrysanthemum, 10 parts of penthorum chinense pursh, 10 parts of dendrobe and 10 parts of Chinese dates. The tea is prepared into tea bags, and each bag is 30g. The traditional Chinese medicine composition is simple in process and low in cost, has a remarkable treatment effect on acute and chronic hepatitis, cholestatic hepatitis, alcoholic hepatitis, fatty liver and the like, and also has the effects of clearing fat, losing weight, inducing diuresis, promoting alcohol metabolism, protecting liver after drinking and the like; the liver health tea bag provided by the invention is rich in nutritional ingredients, very convenient to drink for a long time, high in appearance grade, convenient to use, strong, mellow, sweet and fragrant in taste and worthy of being well developed and utilized, and the products are placed in the tea bag in proportion.

The present invention belongs to the field of molecular biological identification, and specifically relates to a specific SNP site combination and application for identifying Tunchang pig breeds. The specific SNP site combination for identifying Tunchang pig breeds includes 62 SNP sites, and the physical location is based on the reference genome of the pig. Sscrofa11 .1 The specific SNP locus combination was screened based on the whole genome association analysis combined with the selection signal analysis method to ensure the accuracy of the locus selection. The selected specific SNP locus combination can quickly realize the accurate identification of the Tunchang pig breed at the gene level, and has significant application value in the accurate protection of the genetic resources and breed utilization of the Tunchang pig in Hainan.

The invention discloses a selenium-rich dendrobium polysaccharide and a preparation method thereof, wherein the preparation method comprises the following steps: 1) taking a dendrobium officinale extract and adding a nitric acid solution to dissolve it; 2) taking the solution obtained in step 1), adding sodium selenite and barium chloride to react for 6 to 10 hours, cooling and filtering, taking the filtrate to adjust the pH value to 4 to 7, dialyzing, taking the dialyzate and drying, and obtaining the selenium-rich dendrobium polysaccharide. The preparation method of the selenium-rich dendrobium polysaccharide of the invention, through specific synthesis process parameters and a concentration and purification method, the total polysaccharide content in the selenium-rich dendrobium polysaccharide is greater than 90%, and the selenium content is greater than 1.2 mg / g. Experiments have proved that it has significant antioxidant and anti-wrinkle activities.

The invention discloses a reinforced closed heat removal method based on an expansion casing, and relates to the technical field of geothermal energy development, and the method comprises the following steps: selecting a target geothermal reservoir, and determining the depth of a heat removal section according to a geothermal gradient; drilling to the upper part of the heat removal section, installing a common sleeve on the upper part of the heat removal section, and injecting slurry for well cementation as a first open section; after well cementation is completed, drilling continues to be conducted to the lower portion of the heat extraction section of the geothermal reservoir, and reaming is conducted on the heat extraction section; after broaching, an expandable sleeve is installed on the heat removal section, and pressurized expansion treatment is conducted on the expandable sleeve; cement is filled between the well wall and the expandable casing for well cementation to serve as a second open section; and a heat exchange working medium is injected from the first open section annulus, exchanges heat with the second open section and then is extracted from the central pipe, so that geothermal energy utilization is realized. According to the system, the defects that a traditional closed heat removal system is low in efficiency and small in heat removal amount are overcome remarkably, the heat removal temperature is increased, the heat removal energy efficiency is optimized, and development and utilization of medium-deep geothermal energy are accelerated.

The invention belongs to the technical field of soil turning equipment, and particularly relates to soil turning operation equipment suitable for mountains and hills, which comprises a main body carrying frame, a linkage soil turning mechanism and an all-terrain moving system. In order to improve the soil turning efficiency of the hilly environment and reduce the inclination of the equipment, an all-terrain moving system is arranged, a driving motor drives an all-terrain wheel set to rotate, the soil turning equipment can move in an inclined field, meanwhile, the soil turning equipment can stably stop in the field, and a linkage soil turning mechanism can conveniently turn soil on the field; the driving motor is used for pushing the coulter set to turn soil, the coulter set is driven to rotate through the rotating spring after the coulter set is inserted into the land, deep soil is turned out, the soil is looser, circulation of air and water in the soil is facilitated, and growth and development of plant roots are promoted.

The invention belongs to the technical field of food processing, and relates to a processing method for improving the properties of glutinous rice protein by wet heat combined with microwave treatment, comprising: taking an appropriate amount of water and spraying it evenly on the surface of glutinous rice to adjust the moisture content of glutinous rice to 14-16%, sealing for 11-13 hours, drying at 45-55°C for 1-3 hours, cooling to 35-45°C and continuing to dry for 2-4 hours, crushing and sieving; then adding distilled water to glutinous rice flour according to the material-liquid ratio of 1g:4-6ml to prepare a powder emulsion, stirring until there is no obvious precipitation, and performing short-term microwave treatment for multiple times; adjusting the pH to 8-9 with alkali solution, alkali extraction at 50°C for 1.5-2h, taking the supernatant after centrifugation to adjust the acid to near the isoelectric point, standing acid precipitation, centrifuging again, and vacuum freeze drying the precipitate. The process of the invention is simple, and microwave alone can improve the properties of glutinous rice protein, but the combined effect of wet heat and microwave is greater than the single effect, and the modification effect on glutinous rice protein is much greater than the single effect, and the combined effect improves the comprehensive properties of glutinous rice protein and reduces the waste of high-value resources.

The invention discloses a biocontrol marine-source Penicillium and an application thereof, and relates to the field of microbial technology. The biocontrol marine-source Penicillium is named Penicillium sp.DSF059, and is deposited in the China Center for Type Culture Collection, with a deposit number of CCTCC NO: M 20232310. The invention discloses that the biocontrol marine-source Penicillium has a control effect on pathogens of rubber wilt, coconut gray spot, wheat fusarium, cucumber phytophthora root rot, avocado phytophthora root rot, rubber anthracnose, mango anthracnose, sisal zebra pattern, rubber red root, rubber brown root, coffee anthracnose, stylo anthracnose, banana anthracnose, and other tropical crop pathogens. The invention provides a new direction for the control of tropical crop pathogens, and has a good development and application prospect.

The application belongs to the field of molecular biology identification, and particularly relates to a specific SNP site combination for identifying Duntou pig breeds and application. The specific SNP site combination for identifying Duntou pig breeds comprises 40 SNP sites, and the physical position is based on the reference genome of pigs Sscrofa11.1 The sequence comparison is determined. The specific SNP site combination is screened based on the method of whole genome association analysis combined with selection signal analysis, so as to ensure the accuracy of site selection. The specific SNP site combination provided by the application can rapidly realize the accurate identification of Duntou pig breeds at the gene level, and has significant application value in the accurate protection of Hainan local pig genetic resources and breed utilization.

The invention discloses an arbutin derivative 6-O-Caffeoylbutin as well as a preparation method and application of the arbutin derivative 6-O-Caffeoylbutin. The structural formula of a 6-O-Caffeoylbutin compound is as shown in formula (I). The invention also discloses the application of the arbutin derivative (6-O-Caffeoylbutin) disclosed by the invention in preparation of an antioxidant preparation. According to the invention, the 6-O-Caffeoylbutin extracted for the first time shows excellent antioxidant activity, the ABTB free radical scavenging capacity of the 6-O-Caffeoylbutin is higher than that of ascorbic acid and beta-arbutin, and a material basis is provided for developing novel antioxidants.

The invention discloses a compound electrode as well as a preparation method and application thereof, belongs to the technical field of secondary batteries, and solves the problems that an electrode in a secondary battery in the prior art is complicated in preparation process and relatively poor in electrode performance. The compound electrode is composed of a coating and a current collector, and the effective component of the coating comprises a compound active substance; the filter element has a vertically arranged micropore network structure; wherein the compound active substance is a combination of at least two of active materials, and the active materials are positive electrode active materials or negative electrode active materials. The compound electrode provided by the invention is suitable for non-water-sensitive positive and negative electrode materials of a lithium / sodium ion battery, has good broadening performance, and is beneficial to promoting development and utilization of an ultrahigh specific energy secondary battery.

The present application relates to the technical field of microbial application, and in particular to Limosilactobacillus fermentum producing exopolysaccharide and application thereof in hemp seed slurry fermented yogurt. The microbial classification and naming of the Limosilactobacillus fermentum is Limosilactobacillus fermentum GXBMR4, and the preservation number is GDMCC No: 64791. The exopolysaccharide yield of the strain GXBMR4 reaches 288.33 mg / L, the viscosity of the fermentation broth reaches 40.66 mPa·s, and the strain GXBMR4 has safety characteristics such as bile salt tolerance, no hemolyticity, and high antibiotic sensitivity. In addition, the strain GXBMR4 can effectively solve the problems of unstable system and unique "fishy smell" existing in the hemp protein system in the development of hemp protein fermented food. Not only does it promote the development and utilization of hemp protein, but also has high industrial application value in the production of fermented food, and provides a new idea for the development of other plant proteins.

The present invention belongs to the field of molecular biological identification, and specifically relates to a specific SNP site combination and application for identifying the Lingao pig variety. The specific SNP site combination is screened based on a whole genome association analysis combined with a selection signal analysis method, comprising 61 SNP sites, and the physical location is determined by sequence comparison based on the reference genome Sscrofa11.1 of the pig. The specific SNP site combination can accurately identify the unique genetic characteristics of Lingao pigs at the gene level, and can be used for the precise protection of the genetic resources of Hainan Lingao pigs.

The present invention provides a quality control method for a Ruren medicinal material and a Ruren formula granule, the quality control method comprising the following steps: extracting the Ruren medicinal material or the Ruren formula granule to prepare a test solution; mixing protocatechuic acid and an ethanol aqueous solution to prepare a reference solution; respectively detecting the test solution and the reference solution by high performance liquid chromatography to obtain the content of protocatechuic acid in the Ruren medicinal material or the Ruren formula granule and the characteristic spectrum of the Ruren medicinal material or the Ruren formula granule. The present invention constructs an HPLC characteristic spectrum of the Ruren medicinal material and the Ruren formula granule, fully demonstrating the chemical composition characteristics of the Ruren medicinal material and the Ruren formula granule; at the same time, the present invention establishes an HPLC content determination method for protocatechuic acid in the Ruren medicinal material and the Ruren formula granule, which is simple to operate, high in accuracy and good in stability.

The invention relates to a preparation method of high-purity acacetin-7-O-glucuronide, which comprises the following steps of: crushing dry branches and leaves of a verbenaceae plant millettian tree, performing reflux extraction by using a 60-80% methanol solution, combining extracting solutions, and concentrating to obtain an extract; carrying out gel column chromatography on the extract, carrying out gradient elution by using a methanol-water system, detecting by using thin-layer chromatography (TLC), collecting eluent containing acacetin-7-O-glucuronide, merging, and concentrating under reduced pressure to obtain crude fraction of acacetin-7-O-glucuronide. And separating and purifying by using preparative high performance liquid chromatography (PHPLC), and detecting each part of collected eluent by using high performance liquid chromatography (HPLC) to obtain the acacetin-7-O-glucuronide component with the purity of more than 98%. And carrying out TLC and HPLC purity detection and content determination on the obtained product, and establishing a quality control method. The process is simple and reasonable in design, high in separation speed, short in production period, high in product purity, controllable in quality and suitable for industrial production.

The invention discloses a deepwater oil and gas reservoir volume fracturing system and method based on high-strength electric detonation, and relates to the technical field of deepwater oil and gas reservoir fracturing and permeation enhancement, the system comprises a high-voltage power source, a boosting rectifier, an energy storage capacitor bank, a trigger control device and a high-voltage electrode, and the high-voltage power source is used for supplying power to the energy storage capacitor bank; the boost rectifier is used for converting power frequency alternating current into high-voltage direct current; the energy storage capacitor bank is used for storing high-voltage direct current; the trigger control device is used for controlling the on-off state of the whole circuit; the high-voltage transmission cable is used for transmitting the high-voltage direct current stored by the energy storage capacitor bank to the high-voltage electrode; the high-voltage electrode is used for instantaneously releasing the high-voltage direct current stored by the energy storage capacitor bank into the oil and gas reservoir, the reservoir top plate and the reservoir bottom plate, the working efficiency is high, the environment cannot be harmed, and green and efficient deepwater oil and gas reservoir volume fracturing permeation increasing is truly achieved.

The invention provides a rapid propagation method for rodgersia aesculifolia and rodgersia aesculifolia. By controlling a cut block wound surface treatment mode, treatment time, cut block length, cut block thickness, embedding matrix humidity and illumination conditions, cut blocks are induced to bud, and transplanting and field planting are carried out. In the whole cultivation process, phytotoxicity, poison and other chemical influences are not generated on a cultivation medium and seedlings, a positive effect is achieved in the utilization process of important medicinal plants, and the method has good development, utilization, popularization and application prospects in the aspect of utilization of similar plants and is pioneered in China.

The invention belongs to the technical field of medicines, and particularly relates to application of a steroid compound and a pharmaceutical composition. The steroid compound can significantly inhibit excitability of primary cerebral cortex neurons and effectively relieve stimulation of an epilepsy inducer 4-aminopyridine (4-AP) to neurons, and is expected to become an important candidate drug for treating neuron injury and related nervous system diseases.

The invention belongs to but is not limited to the technical field of fermentation, and particularly relates to a method for determining the antioxidant activity of polyphenol of a yeast-fermented tsamba, which obtains an optimal yeast-fermented highland barley tsamba technological formula through optimization on the basis of a single factor in combination with response surface design. Under the optimal formula process condition, the influence of yeast fermentation on polyphenol and antioxidant activity of the tsamba is measured, so that the nutritional value and functional activity of the tsamba are improved. Therefore, a certain theoretical basis is provided for development of highland barley products, the potential market competitiveness of the tsamba is improved, and the tsamba has great significance in protection and inheritance of the traditional national product, namely tsamba.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to an iflurprofen crystal form and a preparation method thereof, and the iflurprofen crystal form is characterized by XRPD (X-Ray Powder Diffraction) and DSC (Differential Scanning Calorimetry). The purity and chiral purity of the crystal form of Efiniprofen provided by the invention are not obviously changed under the conditions of high temperature, high humidity and intense illumination, the crystal form is not converted, and the crystal form has good stability and can form a regular crystal form state, so that the crystal form is beneficial to the technological treatment of drugs, the improvement of physical and chemical properties and the improvement of drug preparation properties; and the method is favorable for transportation, storage and development and utilization of medicinal preparations, and is relatively good in medicinal property, high in yield, simple to operate and suitable for industrial production.

The invention belongs to the technical field of fermentation, and discloses a method for optimizing a formula technology of protogenetic lactic acid bacteria fermented tsamba and determining polyphenol oxidation resistance of the protogenetic lactic acid bacteria fermented tsamba, the optimized fermented tsamba not only has relatively high nutritional value and relatively good taste, but also has relatively good antioxidant activity, and is beneficial to improving the functional activity of the tsamba. The optimal level of the tsamba fermented by lactic acid bacteria is 9.1 mL of buttered tea, 2.7 g of butter, 6.0 g of white granulated sugar and 6.0 g of Qula. The tsamba made according to the formula is neat in structure and shape, fine and uniform in tissue, moderate in hardness, good in taste and best in sensory evaluation. The polyphenol content of the zanba fermented by the lactic acid bacteria is 109.8500 + / -1.070 [mu] mol FAE / g DW, the flavone content of the zanba fermented by the lactic acid bacteria is 0.5568 + / -0.009 mmol CE / g DW, the polyphenol, flavone and antioxidant activity indexes of the zanba fermented by the lactic acid bacteria are obviously higher than those of unfermented zanba, and it is indicated that the polyphenol and flavone content and the polyphenol antioxidant activity of the zanba fermented by the lactic acid bacteria can be improved.

The application belongs to the technical field of biological fermentation, and particularly discloses a phellinus igniarius mycelium, a preparation method and application thereof. The preparation method comprises the following steps: (1) activating the strain: inoculating phellinus igniarius mycelium pieces into PDA culture medium for culture to obtain activated phellinus igniarius; (2) seed culture: inoculating the activated phellinus igniarius into seed culture medium for culture to obtain phellinus igniarius seed liquid; (3) fermentation culture: transferring the phellinus igniarius seed liquid into fermentation culture medium for culture to obtain phellinus igniarius fermentation liquid; and (4) filtration and drying: filtering the phellinus igniarius fermentation liquid to obtain filter residue, washing and drying the filter residue to obtain the phellinus igniarius mycelium. The phellinus igniarius mycelium prepared by the method has high yield and high content of effective components, and can be used as raw material in health products, cosmetics or medicines.

The present invention belongs to the field of molecular biological identification, and specifically relates to a SNP site combination and application for identifying Ding'an pig breeds. The SNP site combination for identifying Ding'an pig breeds includes 53 SNP sites, whose physical locations are based on the reference genome of the pig. Sscrofa11 .1 The SNP locus combination was screened based on the whole genome association analysis combined with the selection signal analysis method, which can quickly realize the accurate identification of Ding'an pig breed at the gene level, and has significant application value in the accurate protection of genetic resources and breed utilization of Hainan Ding'an pig.

The invention discloses a comprehensive treatment method for saline-alkali soil, and belongs to the technical field of saline-alkali soil treatment, the method comprises the following steps: (1) covering the surface of saline-alkali soil under a photovoltaic panel with sand; (2) preparing saline-alkaline tolerant plant seeds into pelletized seeds, and then sowing the pelletized seeds in the sand-covered soil; (3) thoroughly watering after sowing, subsequently managing and protecting according to a conventional method, blocking capillary action by covering sand, reducing the salinity and alkalinity of the earth surface, pelleting the seeds, sowing the pelletized seeds in sandy soil, and ensuring that the seeds can better germinate and grow in the sandy soil to absorb and reduce the salinity of the soil and improve the properties of the soil, so that the soil quality is improved. The photovoltaic panel is matched for shielding to reduce direct solar radiation and surface wind speed, evaporation of surface moisture and accumulation of salt are reduced, comprehensive treatment is carried out on the saline-alkali soil under the combined action, and the treatment efficiency is improved.

The invention discloses a preparation method and a detection method of high-purity Hyuganin D by taking umbelliferae radix peucedani plant radix peucedani sphenanthera as a raw material. The method comprises the following steps: drying and crushing umbelliferae peucedanum plant peucedanum sphenanthera, weighing powder, carrying out ultrasonic extraction by using methanol, carrying out vacuum concentration and drying on a methanol extract, carrying out silica gel column chromatography on an extract, and carrying out gradient elution by using a petroleum ether (60-90 DEG C)-ethyl acetate mixed solution to obtain a Hyuganin D coarse crystal; the method comprises the following steps: collecting a Hyuganin D component with the purity of 90-95% and a Hyuganin D component with the purity of more than 95%, separating by using a reverse phase silica gel C-18 column preparation high performance liquid chromatography, detecting each collected eluent by using analytical high performance liquid chromatography, merging the Hyuganin D component with the same retention time to obtain the Hyuganin D component with the purity of 90-95% and the Hyuganin D component with the purity of more than 95%, and carrying out quality detection by using thin-layer chromatography and liquid chromatography. And the production cost can be reduced.