8results about How to "Promote development and utilization" patented technology

The application relates to a method for hydrothermal acid-controlled degradation of mulberry leaf polysaccharide and antioxidant application thereof, and belongs to the technical field of extraction of active ingredients in natural products. The method comprises the following steps: pretreating raw materials, hydrothermally reacting and acid-catalytically treating mulberry leaves, and alcohol-sedimenting, separating and purifying mulberry leaf polysaccharide, so as to obtain mulberry leaf polysaccharide, finally, the antioxidant application of the mulberry leaf polysaccharide is explored, and a theoretical basis is provided for development of mulberry leaf resources. The method can not only obtain mulberry leaf polysaccharide, but also obtain high-value natural active ingredients such as rutin, astragalin, scopoletin and isoquercitrin in the mulberry leaves, is beneficial to development and utilization of the mulberry leaf resources, meets the needs of people's health, and has practical significance and economic value.

The application belongs to the field of molecular biology identification, and particularly relates to a specific SNP site combination for identifying Duntou pig breeds and application. The specific SNP site combination for identifying Duntou pig breeds comprises 40 SNP sites, and the physical position is based on the reference genome of pigs Sscrofa11.1 The sequence comparison is determined. The specific SNP site combination is screened based on the method of whole genome association analysis combined with selection signal analysis, so as to ensure the accuracy of site selection. The specific SNP site combination provided by the application can rapidly realize the accurate identification of Duntou pig breeds at the gene level, and has significant application value in the accurate protection of Hainan local pig genetic resources and breed utilization.

The invention discloses an arbutin derivative 6-O-Caffeoylbutin as well as a preparation method and application of the arbutin derivative 6-O-Caffeoylbutin. The structural formula of a 6-O-Caffeoylbutin compound is as shown in formula (I). The invention also discloses the application of the arbutin derivative (6-O-Caffeoylbutin) disclosed by the invention in preparation of an antioxidant preparation. According to the invention, the 6-O-Caffeoylbutin extracted for the first time shows excellent antioxidant activity, the ABTB free radical scavenging capacity of the 6-O-Caffeoylbutin is higher than that of ascorbic acid and beta-arbutin, and a material basis is provided for developing novel antioxidants.

The present application relates to the technical field of microbial application, and in particular to Limosilactobacillus fermentum producing exopolysaccharide and application thereof in hemp seed slurry fermented yogurt. The microbial classification and naming of the Limosilactobacillus fermentum is Limosilactobacillus fermentum GXBMR4, and the preservation number is GDMCC No: 64791. The exopolysaccharide yield of the strain GXBMR4 reaches 288.33 mg / L, the viscosity of the fermentation broth reaches 40.66 mPa·s, and the strain GXBMR4 has safety characteristics such as bile salt tolerance, no hemolyticity, and high antibiotic sensitivity. In addition, the strain GXBMR4 can effectively solve the problems of unstable system and unique "fishy smell" existing in the hemp protein system in the development of hemp protein fermented food. Not only does it promote the development and utilization of hemp protein, but also has high industrial application value in the production of fermented food, and provides a new idea for the development of other plant proteins.

The invention relates to a preparation method of high-purity acacetin-7-O-glucuronide, which comprises the following steps of: crushing dry branches and leaves of a verbenaceae plant millettian tree, performing reflux extraction by using a 60-80% methanol solution, combining extracting solutions, and concentrating to obtain an extract; carrying out gel column chromatography on the extract, carrying out gradient elution by using a methanol-water system, detecting by using thin-layer chromatography (TLC), collecting eluent containing acacetin-7-O-glucuronide, merging, and concentrating under reduced pressure to obtain crude fraction of acacetin-7-O-glucuronide. And separating and purifying by using preparative high performance liquid chromatography (PHPLC), and detecting each part of collected eluent by using high performance liquid chromatography (HPLC) to obtain the acacetin-7-O-glucuronide component with the purity of more than 98%. And carrying out TLC and HPLC purity detection and content determination on the obtained product, and establishing a quality control method. The process is simple and reasonable in design, high in separation speed, short in production period, high in product purity, controllable in quality and suitable for industrial production.

The application belongs to the technical field of biological fermentation, and particularly discloses a phellinus igniarius mycelium, a preparation method and application thereof. The preparation method comprises the following steps: (1) activating the strain: inoculating phellinus igniarius mycelium pieces into PDA culture medium for culture to obtain activated phellinus igniarius; (2) seed culture: inoculating the activated phellinus igniarius into seed culture medium for culture to obtain phellinus igniarius seed liquid; (3) fermentation culture: transferring the phellinus igniarius seed liquid into fermentation culture medium for culture to obtain phellinus igniarius fermentation liquid; and (4) filtration and drying: filtering the phellinus igniarius fermentation liquid to obtain filter residue, washing and drying the filter residue to obtain the phellinus igniarius mycelium. The phellinus igniarius mycelium prepared by the method has high yield and high content of effective components, and can be used as raw material in health products, cosmetics or medicines.

The invention relates to a preparation method of high-purity scutellarein 4 '-methyl ether-7-O-glucoside acid, which comprises the following steps of: crushing dry branches and leaves of a verbenaceae plant mirabilis, performing reflux extraction by using a 60%-80% methanol solution, combining extracting solutions, and concentrating to obtain an extract; carrying out macroporous adsorption resin chromatography on the extract, carrying out gradient elution by using a methanol-water system, detecting by using thin-layer chromatography (TLC), collecting eluent containing scutellarein 4 '-methyl ether-7-O-glucoside, merging, and concentrating under reduced pressure to obtain a crude fraction; and separating and purifying by using a preparative high performance liquid chromatography (PHPLC) method, and detecting each part of collected eluent by using high performance liquid chromatography (HPLC) to obtain the scutellarein 4 '-methyl ether-7-O-glucoside component with the purity of more than 98%. And carrying out TLC and HPLC purity detection and content determination on the obtained product, and establishing a quality control method. The method is reasonable in design, simple in process, high in separation speed, short in production period, high in product purity, controllable in quality and suitable for industrial production.

The application discloses a method for improving gas production performance of straw and pig manure mixture dry biogas fermentation based on multi-factor cooperation, which comprises the following steps: mixing pig manure and straw at a ratio of 1:1 as the best feed substrate according to total solids, adopting semi-continuous dry biogas fermentation process, feeding the substrate into a biogas fermentation reactor, inoculating anaerobic sludge domesticated by pig manure, controlling the total solid concentration of the feed substrate to be 20%-30%, controlling the hydraulic retention time to be 25-45 days, feeding air into the fermentation process for air stirring, controlling the stirring intensity to be 33.3-66.7 mL / (L.min), and stirring once a day for 1 minute each time. The straw and pig manure mixture dry biogas fermentation gas production performance can be effectively improved through the synergistic effect of the optimized raw material ratio, the total solid concentration of the feed, the hydraulic retention time and the air stirring intensity.