The invention discloses a preparation process of a Qingjin
phlegm-reducing decoction material standard, an HPLC
content determination method and a
fingerprint spectrum establishment method aiming at the material standard, under the
content determination method, the peak shapes of chromatographic peaks of all components are symmetrical, the separation effect is remarkable, and excellent stability, specificity, linear response,
repeatability, precision and accuracy are shown. According to the present invention, the
fingerprint characteristics are accurately recorded, the reversed-phase
high performance liquid chromatography technology is adopted to successfully obtain 13 common peaks, the
baicalin is adopted as the reference peak, the relative
retention time and the relative
peak area of the common peaks are calculated, and the reference substance comparison is further performed to identify the 8 common peaks; the established
fingerprint spectrum method also has the characteristics of good chromatographic peak shape, high separation degree, stable method, excellent
repeatability, high precision and the like. In conclusion, the invention provides a set of comprehensive and reliable Qingjin
phlegm-reducing decoction material standard
quality control reference
system, and a scientific basis is provided for the overall quality evaluation of the Qingjin
phlegm-reducing decoction.