106 results about "Thin layer chromatographic" patented technology

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

This invention discloses a quality control method for an anti-snoring oral liquid and its application, belonging to the field of traditional Chinese medicine quality testing technology. The method includes thin-layer chromatography identification of Gleditsia sinensis thorns, Scrophularia ningpoensis, and Glycyrrhiza uralensis, as well as high-performance liquid chromatography (HPLC) determination of hesperidin content. By optimizing pretreatment, developing solvent, and colorimetric conditions, this invention achieves efficient and specific identification and quantification of multiple components in the compound preparation. The method is highly specific, produces clear spots, has good separation, and is tolerant to a wide temperature and humidity range (2-40℃, 20%-75%RH), requiring no strict environmental control, significantly improving detection efficiency and providing a reliable basis for establishing quality standards for this preparation.

The invention relates to a method for detecting a clearing agent main agent in gasoline, and the method comprises the following steps: carrying out thin-layer chromatography detection on a to-be-detected sample, and determining the composition of the clearing agent main agent in the concentrated to-be-detected sample according to color spot distribution on an obtained thin-layer chromatography plate, wherein the clearing agent main agent comprises one or more of a polyether amine clearing agent, a polyisobutene amine clearing agent and a Mannich base clearing agent; the thin-layer chromatography detection sequentially comprises developing and developing treatment; a developing agent for developing comprises one or more of a developing agent I, a developing agent II and a developing agent III; the developing solvent I is methanol, and the developing solvent II and the developing solvent III comprise one or more of alcohol with the carbon atom number of 1-6, aliphatic hydrocarbon with the carbon atom number of 5-12 and cycloalkane with the carbon atom number of 5-12. According to the method provided by the invention, whether the clearing agent main agent is added into the sample to be detected or not can be rapidly determined, and the type of the clearing agent main agent can be rapidly and accurately detected.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to stasis-removing and pain-relieving pills and a quality detection method thereof. The stasis-removing and pain-relieving pill is prepared by taking radix paeoniae alba, rhizoma corydalis, radix paeoniae rubra, angelica sinensis, ligusticum wallichii, rhizoma cyperi, rhizoma zingiberis, fennel and astragalus membranaceus as raw materials and taking 10% starch slurry as an adhesive. The quality detection method of the stasis-removing and pain-relieving pill comprises general inspection, microscopic identification and thin-layer chromatography identification. And high performance liquid chromatography content determination. According to the present invention, by researching the preparation process and the quality standard of the stasis removing and pain relieving pill, the new thought and the new method are provided for the development of the traditional Chinese medicine preparation, the excellent choice is hopefully provided for the common gynecologic symptoms such as irregular menstruation, dysmenorrhea, postpartum blood stasis abdominal pain and the like, and the pain caused by the illness is relieved.

Disclosed is a thin-layer chromatography identification method for Rhizoma dioscoreae hypoglaucae formula granules. The method comprises: taking a sample to be subjected to detection, and dissolving s

The invention discloses a preparation method of a dialkynyl silane compound, which belongs to the technical field of organic synthesis and comprises the following steps: sequentially adding a reducing agent, an organic solvent, alkynyl bromide and chlorosilane into a reaction container, and reacting at 20-60 DEG C for 6-36 hours to obtain a mixture A; wherein R1 is allyl, alkyl or aryl; wherein R2 is allyl, alkyl or aryl; wherein R3 is an alkenyl group, an alkyl group, an alkoxy group or a silicon group; wherein R is halogen, alkyl, aryl, alkoxy, ester group, cyano or carbonyl; diluting the mixture A with ethyl acetate, adding silica gel powder, and performing vacuum concentration to remove the reaction solvent to obtain a mixture B; and separating the mixture B through thin-layer chromatography or column chromatography to obtain the target product dialkynyl silane compound. According to the method, dialkynyl silane substituted by different alkynyl groups can be obtained at the same time, the chemical selectivity is good, and dialkynyl silane which is not easy to obtain by other methods can be synthesized.

This invention belongs to the field of traditional Chinese medicine detection technology, specifically relating to a multi-component thin-layer chromatography (TLC) identification method for pediatric runtong syrup. This invention establishes specific TLC identification processes for roasted radish seed, immature bitter orange, hemp seed, and scrophularia root, optimizing the sample pretreatment, developing solvent ratio, spotting volume, and colorimetric inspection conditions for each component. After specificity and robustness studies, the method demonstrates good stability under varying temperature, humidity, TLC plate type, and spotting volume ranges, exhibiting no negative interference and clear spots, enabling accurate multi-component identification. This invention is the first to establish a TLC identification method for these four medicinal materials in pediatric runtong syrup, providing a scientific and reliable technical means for the quality control of this compound preparation and filling a gap in existing technology.

The invention relates to the technical field of cistanche deserticola detection, in particular to a method for simultaneously and rapidly detecting four phenylethanoid glycosides in cistanche deserticola, which comprises the following steps: preparing a plurality of parts of mixed reference substance solutions containing four phenylethanoid glycosides with known concentrations; preparing a to-be-detected cistanche sample into a test solution; spotting the mixed reference substance solution and the test solution on the same thin-layer plate, carrying out thin-layer chromatography development by adopting a developing solvent, and volatilizing the solvent; placing the thin-layer plate after the solvent is volatilized under ultraviolet light to collect spot images, and processing the images by adopting image analysis software to obtain integral gray values of all phenylethanoid glycoside spots; respectively drawing a standard curve by taking the known concentrations of the four phenylethanoid glycosides in each mixed reference substance solution as horizontal coordinates and the corresponding integral gray values as vertical coordinates, establishing a linear regression equation, and calculating the contents of the four phenylethanoid glycosides in the cistanche deserticola sample to be detected; the invention provides a rapid and accurate method for determining the content of four phenylethanoid glycosides in cistanche.

This invention provides a sample pretreatment method and identification method for identifying ginseng polysaccharides in compound fluorouracil oral solution, belonging to the field of pharmaceutical detection and analysis technology. The sample pretreatment method of this invention includes: centrifuging the compound fluorouracil oral solution and taking the clear solution; adding silica gel to the clear solution, mixing, and removing the solvent to obtain a solid mixture; adding ethanol solution or anhydrous ethanol to the obtained solid mixture, heating and refluxing, discarding the ethanol solution, and obtaining the residue; adding water to the obtained residue, sequentially extracting and centrifuging, taking the supernatant obtained by centrifugation, removing the solvent from the supernatant, and then redissolving it with water; the identification method of this invention is thin-layer chromatography analysis, and the developing solvent includes the following components in parts by volume: 6.8-9.2 parts of n-propanol, 1.76-2.24 parts of water, 0.75-1.24 parts of ethyl acetate, and 0.75-1.24 parts of glacial acetic acid. The sample pretreatment method and identification method for identifying ginseng polysaccharides in compound fluorouracil oral solution of this invention have high specificity.

The present application relates to the technical field of pharmaceutical analysis, and in particular to a thin-layer identification method for Qingxiangteng formula granules and standard decoction, which comprises the following steps: subjecting test sample solution and control medicinal material solution to thin-layer chromatography analysis, and comparing the obtained chromatograms, wherein the developing agent is a mixed solution of chloroform, methanol and formic acid in a volume ratio of (5-7):(0.5-2):(0.4-0.6). The present application is simple to operate, free of negative interference, and clear in color development, and is specific and durable, thus being an improvement on the existing Qingxiangteng preparation identification method.

The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a thin-layer chromatography identification method for 8 medicines in 11 medicines in the whole formula of Qingjin phlegm-reducing decoction. The core of the method is that three different test solutions are adopted to realize the identification of eight medicines, namely scutellaria baicalensis, rhizoma anemarrhenae, exocarpium citri rubrum, fructus gardeniae, liquorice, thunberg fritillary bulb, platycodon grandiflorum and radix ophiopogonis. Wherein the first test solution is prepared by extracting water-saturated n-butyl alcohol, and can be simultaneously used for identifying scutellaria baicalensis, rhizoma anemarrhenae, exocarpium citri rubrum, fructus gardeniae and liquorice; the second solution is subjected to ammoniation and dichloromethane extraction to be used for thunberg fritillary bulb identification; the third solution is extracted by dichloromethane after being hydrolyzed by hydrochloric acid, and platycodon grandiflorum and radix ophiopogonis can be identified at the same time. According to the method, by optimizing a test sample preparation and development system, sharing of test samples of multiple medicines and synchronous identification are achieved, the detection efficiency is remarkably improved, the method has the advantages of being easy and convenient to operate, clear in spot, good in separation degree, high in specificity, negative, free of interference and the like, and a reliable basis is provided for quality control of the Qingjin phlegm reducing decoction.

This invention belongs to the technical field of qualitative determination of ephedra, licorice, and bitter almond in traditional Chinese medicine compound preparations. This invention discloses a method for simultaneously identifying ephedra, licorice, and bitter almond in Ma Xing Shi Gan oral liquid. The test sample is placed in a Soxhlet extractor and treated with reagents of different properties to prepare a fractional extraction solution. Ephedra and licorice reference materials are then processed to obtain a reference material solution. Amygdalin reference standard is prepared by adding methanol to prepare a reference solution. The above test sample solution, reference material solution, and reference solution are used in a thin-layer chromatography system to simultaneously identify ephedra, licorice, and bitter almond. The results show that the chromatographic spots are well separated and clear, with no interference from the negative control. This method has simple pretreatment, is environmentally friendly, has high sensitivity, and its repeatability and robustness meet the requirements of the Chinese Veterinary Pharmacopoeia, thus enabling better quality control of Ma Xing Shi Gan oral liquid.

The invention discloses a rapid detection method of isobutyramido thiazolyl resorcinol in cosmetics, and belongs to the technical field of safety detection and analysis of cosmetics. Thin-layer chromatography is taken as a main line, separation, 254nm and 365nm dual-band ultraviolet observation, 105 DEG C thermal activation enhancement and three-channel color development recheck of ferric trichloride, vanillin-sulfuric acid and phosphotungstic acid are sequentially completed, and the detection result is accurate. The method comprises the following steps: acquiring multilayer evidences of physical separation, optical response and chemical reaction, establishing a standardized ratio shift value according to upper and lower interior labels and a solvent front edge, extracting indexes such as brightness and color difference, dispersing displacement and intensity signals into five-bit bar codes, realizing fault-tolerant matching by using a Hamming distance, outputting positive, confirmation or negative conclusions, and determining the accuracy of the result. And generating a report containing the original image and the parameters. The method is oriented to complex matrixes such as emulsion, face cream, essence, mask liquid, lipstick and eye shadow, by means of multi-mechanism cross validation and bar code type fusion interpretation, the false positive and false negative risks are remarkably reduced, and the result consistency and the rechecking efficiency are improved.

The application discloses a method for detecting clenbuterol based on thin layer chromatography and surface enhanced resonance Raman spectroscopy, and belongs to the technical field of food detection. The method comprises the following steps: (1) adopting thin layer chromatography to preliminarily separate the clenbuterol component from the matrix in the pig liver sample, and confirming the clenbuterol component in the pig liver sample under the ultraviolet lamp of 254 nm; (2) adding aromatic diazonium salt solution to the same Rf value of the clenbuterol component reference sample spot to generate azo compounds, and then adding a surface enhancer silver sol; (3) in-situ detecting the surface enhanced resonance Raman spectrum of the corresponding azo compound component spot, and if the surface enhanced resonance Raman spectrum of the pig liver sample solution at the same Rf value is the same as that of the reference sample solution, it is determined that the pig liver sample contains the clenbuterol component. The application has high specificity for detecting clenbuterol and salbutamol, high sensitivity, fast analysis speed, and is beneficial to fast detection of grass-roots units. The application is suitable for detecting clenbuterol.

The invention provides a method for identifying and detecting a phlegm stasis and arthralgia dredging prescription. The method comprises three modules of construction of a characteristic chromatogram of a medicinal material and optional thin-layer chromatography identification and effective component content determination. The construction of the characteristic spectrum adopts a diethyl ether-ethyl acetate two-stage segmented extraction UPLC analysis strategy, so that the interference of complex components is overcome, and the overall chemical characteristics of the compound can be comprehensively represented; thin-layer chromatography identification provides a specific identification method for medicines such as coptis chinensis, curcuma wenyujin and vinegar-processed rhizoma corydalis; and the content determination adopts a high performance liquid chromatography to detect the effective components chlorogenic acid, salvianolic acid B, glycyrrhizic acid and berberine hydrochloride. According to the invention, a multi-dimensional and configurable quality control system is constructed, the comprehensive quality control of the phlegm stasis and arthralgia dredging prescription from qualitative and overall characterization to quantitative analysis is realized, the specificity is strong, and the result is reliable.

The invention provides a quality standard of a rungia pectinata medicinal material and a detection method thereof, and relates to the technical field of traditional Chinese medicines. The invention provides a quality standard detection method for Rungia pectinata medicinal materials, which detects the quality of Rungia pectinata from the aspects of medicinal material sources, character identification, microscopic identification, thin-layer identification, moisture, total ash content, acid-insoluble ash content and extracts, and establishes a thin-layer chromatography identification method with strong specificity, stability and reliability. Therefore, the authenticity of the rungia pectinata medicinal material is identified, the quality of the rungia pectinata medicinal material is evaluated, and the medication safety is ensured. According to the quality standard of the Rungia pectinata medicinal material established by the method, the identification result is stable and reliable, and the accuracy is high.

The utility model provides a kind of thin layer chromatogram sample application device, including base, the base is provided with sample application platform, the sample application platform is provided with sample application seat, the one side of the sample application platform is provided with support, the support is provided with sample application head;The base is provided with intermittent drive assembly, the intermittent drive assembly is matched with the sample application platform;The inside of base is provided with support frame, the rotating motor passes through the support frame and is connected with the drive shaft, the drive shaft is connected with the drive gear, one end of the transmission shaft is connected with the support frame, and the other end passes through the transmission gear and is connected with the driving element, the transmission gear is engaged with the drive gear.The thin layer chromatogram sample application device provided by the utility model is provided with intermittent drive assembly, the intermittent rotation of sample application platform is driven by gear engagement driving element, continuous sample application can be realized, and sample application efficiency is improved.

The invention relates to the technical field of analysis and detection, and particularly discloses a thin-layer identification method for Kangyanling granular lotus seeds. The thin-layer identification method for the Kangyanling granular lotus seeds comprises the following steps: preparing a test solution, preparing a reference medicinal material solution and performing thin-layer chromatography. The preparation method has the advantages of reducing interference of interference spots on determination of main spots of a sample and improving specificity.

The quality detection method is characterized by comprising a thin-layer chromatography identification method for radix salviae miltiorrhizae and radix angelicae sinensis and / or a method for determining the content of radix salviae miltiorrhizae by adopting liquid chromatography. By optimizing the thin-layer chromatography method, efficient and specific identification of salvia miltiorrhiza and angelica sinensis in the ganoderma lucidum and ginseng oral liquid is realized, and a reliable technical scheme is provided for quality control of a traditional Chinese medicine preparation; the method passes methodology verification (specificity, durability and the like) and is stable in stability investigation, the quality safety of the ganoderma lucidum and ginseng oral liquid is further ensured, and evaluation is comprehensive.

The application relates to the technical field of biological medicine, and discloses a preparation method of a hederosapogenin STING channel inhibitor, which comprises the following steps: taking appropriate hederosapogenin and aromatic compounds, adding N,N-dimethylformamide as a reaction solvent, and adding appropriate K2CO3; stirring under a heating reflux state for a certain time, monitoring the reaction process through thin layer chromatography until the reaction is completed; after the reaction is completed, the N,N-dimethylformamide is evaporated under reduced pressure, the obtained residue is dissolved in dichloromethane, and the residue is extracted and washed with deionized water and saturated brine in sequence; the organic layer is collected, dried with anhydrous sodium sulfate, filtered, evaporated under reduced pressure, and a crude product is obtained; the crude product is separated and purified through a silica gel column chromatography, and a target compound, namely a STING channel inhibitor derivative, is obtained. The hederosapogenin is chemically modified to synthesize a derivative with high STING channel activity, which is used for treating immune and inflammation related diseases.

A method for evaluation of a thin-layer chromatography plate after performing a separation process that separates components of a sample on the thin-layer chromatography plate comprises a digitization step, wherein at least two digital images are taken that differ with respect to the wavelength range of illumination of the thin-layer chromatography plate. The method further comprises a position identification step wherein the position of the thin-layer chromatography plate is identified for each of the at least two digital images. Furthermore, the method comprises an evaluation step wherein an image information of the at least two digital images is superimposed for at least all regions with at least one visible spot within at least one of the at least two digital images, so that the superimposition of image information from identical regions of at least two digital images can be used for evaluation of the thin-layer chromatography.

The present application relates to the technical fields of medical and pharmaceutical chemical synthesis, and particularly relates to a synthesis method of 3-thiocyanatospiro cyclobutyl oxindole compounds, which comprises the following steps: in a reactor, compound 1, compound 2, a catalyst, an additive and a solvent are stirred for 30 minutes at room temperature, after the reaction is completed, the crude product is obtained by reduced pressure distillation, and the 3-thiocyanatospiro cyclobutyl oxindole compound is obtained by column chromatography and thin layer chromatography purification. N The present application takes phenyl bicyclo[1.1.0]butane-1-formamide compounds and thiocyanate as raw materials, and promotes the synthesis of 3-thiocyanatospiro cyclobutyl oxindole compounds by an oxidant. The present application has the advantages of simple synthesis steps, mild conditions, safe operation, good functional group compatibility, high reaction conversion, low cost and the like, and is conducive to industrial production.

This invention discloses a method for preparing diosmin impurity compounds and their application, relating to the field of pharmaceutical impurity preparation technology. The preparation method includes the following steps: S1, preparation of compound IM-1: compound SM-1 and a methylating agent are heated under alkaline conditions to obtain compound IM-1; S2, preparation of compound formula 1: compound IM-1 is added to an alcohol solution, and an inorganic base is added under stirring. After the addition is complete, the mixture is kept warm and stirred, then cooled to 15-20°C, and dilute hydrochloric acid is added dropwise until the pH reaches 6.5-7. The mixture is concentrated to obtain the crude product of formula 1, and finally purified by thin-layer chromatography to obtain the pure product of formula I. This product can be used as a reference standard for the quality control of diosmin raw material, which helps to obtain a higher quality product.

The invention discloses a thin-layer chromatography identification method and application of radix cynanchi auriculati traditional Chinese medicine formula granules and radix cynanchi auriculati traditional Chinese medicine formula granules as well as a preparation method of the radix cynanchi auriculati traditional Chinese medicine formula granules and the radix cynanchi auriculati traditional Chinese medicine formula granules, and relates to the technical field of detection methods. The method comprises the following steps: dripping a test solution on a silica gel G thin-layer plate, and developing and inspecting under thin-layer chromatography conditions; thin-layer chromatography conditions are as follows: n-hexane-ethyl formate-formic acid is used as a developing solvent; the volume ratio of the normal hexane to the ethyl formate to the formic acid is 20: 15: 1; the spreading temperature is 4-26 DEG C, and the spreading humidity is 30% rh to 60% rh; compared with a thin-layer chromatogram of the radix cynanchi auriculati traditional Chinese medicine formula granules, the radix cynanchi auriculati charcoal traditional Chinese medicine formula granules have fluorescent spots at the specific shift value of 0.421-0.458. The method can be used for rapidly and effectively identifying the radix cynanchi bungei traditional Chinese medicine formula granules or radix cynanchi bungei charcoal traditional Chinese medicine formula granules which lose the form of the decoction pieces, is simple and convenient to operate, high in precision and sensitivity and good in stability, and has a good application prospect.

The invention relates to the technical field of general trace detection, in particular to a TLC (thin-layer chromatography) detection method for pharmaceutical ingredients of QAMS (quantitative analysis of multi-components by single marker) in heat-clearing and detoxifying granules, which comprises the following steps: performing thin-layer chromatography detection on a plurality of to-be-detected solutions prepared from to-be-detected drugs; performing closed graph detection on the target image to obtain a graph area; performing a first matching operation on the graphic area and a corresponding historical area in the historical image to obtain a first trailing value; performing second matching operation on the target graph area and the corresponding target historical area to obtain a second trailing value; and determining a detection optimization strategy for thin-layer chromatography detection according to a trailing classification result corresponding to the second trailing value of the target graphic area, and obtaining a single-detection multi-evaluation result of the to-be-detected liquid based on the detection optimization strategy. According to the invention, the operation speed and the accuracy of the TLC detection method for the medicinal components for quantitative analysis of multi-components by single marker in the heat-clearing and detoxifying granules are improved.

This invention relates to a method for preparing 6-shogaol from chili peppers. It solves the technical problems of existing 6-shogaol extraction and purification methods, such as large solvent consumption and difficulty in recovery, significant safety hazards, unsuitability for large-scale production, cumbersome processes, long processing times, and low yields. The method of this invention includes the following steps: S1) Extracting and separating chili peppers using a supercritical CO2 method to obtain an oil paste; S2) Dissolving and saponifying with alkaline water, then concentrating to obtain a clear and transparent oil; S3) Performing molecular distillation to obtain a heavy phase component oil; S4) Eluting on a first silica gel column for adsorption, while simultaneously using thin-layer chromatography to detect the eluent, collecting the mixed eluent containing 6-shogaol, and concentrating to obtain a light red oily substance; S5) Eluting on a second silica gel column for adsorption, while simultaneously using thin-layer chromatography to monitor the eluent, collecting the eluent containing only a single point of 6-shogaol, and concentrating to obtain a light yellow oily substance; S6) Dissolving, decolorizing, and crystallizing at low temperature to obtain the 6-shogaol product.

The application provides a preparation method of high-purity 5''-O-acetyllobinan, and belongs to the technical field of chemical reference substance preparation.The method comprises the following steps: (1) extracting powder of Millettia pachycarpa Benth with ethanol, collecting a filtrate, and concentrating to obtain an extract; (2) adding water to the extract to prepare a suspension, extracting and concentrating the suspension to obtain an extraction part; (3) separating the obtained extraction part through gradient elution of a silica gel column chromatography and elution of a dextran gel column chromatography, collecting a flow fraction containing 5''-O-acetyllobinan, combining the flow fraction after thin layer chromatography detection, concentrating and recrystallizing to obtain a crude crystal of 5''-O-acetyllobinan; and (4) purifying the crude crystal through preparative liquid chromatography, collecting a flow fraction containing 5''-O-acetyllobinan, detecting the flow fraction through high performance liquid chromatography, combining flow fractions with the same retention time and higher purity, and concentrating and drying to obtain 5''-O-acetyllobinan with a purity greater than 98%.

The present application relates to the technical field of drug efficacy detection, and particularly relates to a test method of Suguanning capsules. The present application adds the detection standards of Isatis indigotica Fort, Siphonostegia chinensis Maxim, Lonicera japonica and paracetamol and Bupleurum chinense to the original standard of Suguanning capsules, carries out thin layer chromatography on Isatis indigotica Fort, Siphonostegia chinensis Maxim and Lonicera japonica, and carries out the detection of specificity, linear relationship, precision, stability, reproducibility and sample recovery rate on paracetamol and Bupleurum chinense. The thin layer chromatography reproducibility and specificity of Bupleurum chinense and Lonicera japonica are good, and the negative has no interference, so the quality standard text is included. The thin layer chromatography specificity of Siphonostegia chinensis Maxim and Isatis indigotica Fort is poor, so the quality standard text is not included, and the detection standard of Suguanning capsules is improved.

The application discloses a thin layer chromatography method for isolineatin and procyanidin B2 in folium artemisiae and products thereof, and comprises the following steps: using a methanol-water solution with pH of 10-11 as an extractant to prepare a test sample solution; using methanol as a solvent to prepare a control sample solution; respectively sucking the test sample solution and the control sample solution, spotting on a thin layer plate, using dichloromethane-normal propyl alcohol-water-glacial acetic acid as a developing agent to develop, and observing under a UV lamp after air-drying. The application successfully establishes a thin layer chromatography rapid detection method for isolineatin and procyanidin B2 in folium artemisiae and products thereof, which is not only simple in operation, but also can realize semi-quantitative analysis of isolineatin and procyanidin B2, and provides a powerful detection means for quality control of folium artemisiae and products thereof.