208 results about "Thin layer chromatographic" patented technology

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention discloses a compound donkey-hide gelatin pulp thin-layer chromatography fingerprint construction and combination evaluation analysis method, and belongs to the technical field of traditional Chinese medicine quality control. The compound colla corii asini pulp thin-layer chromatography is a thin-layer chromatography fingerprint spectrum of the compound colla corii asini pulp or an intermediate in a process preparation process; the construction of the thin-layer chromatography fingerprint spectrum is based on the color of a thin-layer chromatography sample strip image and the gray scale of spots, and a pixel-intensity matrix and a ratio shift value-gray scale matrix are formed to comprehensively reflect the thin-layer chromatography characteristics of the sample; according to the combined evaluation method of the thin-layer chromatography fingerprint spectrum, a method of combining machine learning with information entropy and entropy is adopted, ginsenoside Rg1, codonopsis pilosula alkynol and other components are screened and identified as key components influencing the compound donkey-hide gelatin pulp and transmission, and the method has the characteristics of accuracy and high efficiency, is wide in application prospect and can provide a thought for establishment of quality standards of traditional Chinese medicine compounds.

The invention discloses a thin-layer chromatography identification method for cinnamon and ginger in vitality-preserving granules, and belongs to the technical field of pharmaceutical analysis. Aiming at the problems of step-by-step detection, serious cinnamic acid tailing and dependence on toxic solvents in the existing method, the method comprises the following steps: dissolving vitality-preserving particles in water, carrying out ultrasonic treatment, extracting filtrate by using absolute ether, merging extract liquor, evaporating to dryness, and dissolving by adding ethyl acetate to obtain a test solution; preparing a cinnamon reference medicinal material solution and a cinnamic acid and 6-gingerol reference substance solution; a silica gel GF254 thin-layer plate is adopted, petroleum ether-normal hexane-ethyl formate-formic acid is used as a developing solvent for developing, and inspection is conducted under the ultraviolet light of 254 nm and the ultraviolet light of 365 nm respectively. Cinnamon and ginger can be synchronously identified, spots are clear and free of trailing, no toxic solvent exists, durability is high, cost is low, and an accurate and reliable basis can be provided for quality control of the vitality-preserving granules.

The application discloses a kind of gardenia and its processed product identification method, comprising: step one, respectively take equal amount of gardenia sample powder, gardenia processed product sample powder;Step two, the above-mentioned powder is respectively soaked with ethanol solution, then ultrasonic extraction is carried out, filtration, filter liquor is evaporated to dryness, obtain gardenia sample crude extract, gardenia processed product sample crude extract;Step three, the above-mentioned crude extract is respectively dissolved with water, then sequentially extracted with petroleum ether, ethyl acetate twice, and the two extraction liquids are combined and evaporated to dryness, obtain extract, the extract is dissolved with methanol, obtain gardenia sample test solution, gardenia processed product sample test solution;Step four, the above-mentioned test solution is respectively spotted on the same silica gel thin layer plate, with developing agent development, dry, with color developing agent color development, and under ultraviolet lamp, view thin layer chromatogram, according to the number, position, color of fluorescence spot on thin layer chromatogram, identify sample variety.

This invention discloses a quality control method for an anti-snoring oral liquid and its application, belonging to the field of traditional Chinese medicine quality testing technology. The method includes thin-layer chromatography identification of Gleditsia sinensis thorns, Scrophularia ningpoensis, and Glycyrrhiza uralensis, as well as high-performance liquid chromatography (HPLC) determination of hesperidin content. By optimizing pretreatment, developing solvent, and colorimetric conditions, this invention achieves efficient and specific identification and quantification of multiple components in the compound preparation. The method is highly specific, produces clear spots, has good separation, and is tolerant to a wide temperature and humidity range (2-40℃, 20%-75%RH), requiring no strict environmental control, significantly improving detection efficiency and providing a reliable basis for establishing quality standards for this preparation.

The invention relates to a method for detecting a clearing agent main agent in gasoline, and the method comprises the following steps: carrying out thin-layer chromatography detection on a to-be-detected sample, and determining the composition of the clearing agent main agent in the concentrated to-be-detected sample according to color spot distribution on an obtained thin-layer chromatography plate, wherein the clearing agent main agent comprises one or more of a polyether amine clearing agent, a polyisobutene amine clearing agent and a Mannich base clearing agent; the thin-layer chromatography detection sequentially comprises developing and developing treatment; a developing agent for developing comprises one or more of a developing agent I, a developing agent II and a developing agent III; the developing solvent I is methanol, and the developing solvent II and the developing solvent III comprise one or more of alcohol with the carbon atom number of 1-6, aliphatic hydrocarbon with the carbon atom number of 5-12 and cycloalkane with the carbon atom number of 5-12. According to the method provided by the invention, whether the clearing agent main agent is added into the sample to be detected or not can be rapidly determined, and the type of the clearing agent main agent can be rapidly and accurately detected.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

The present invention discloses a thin layer chromatography identification method for simultaneously identifying multiple components in a refined flat peach pill preparation and its application, which belongs to the technical field of quality control of traditional Chinese medicine. The thin layer chromatography identification method of the present invention is divided into two parts. The first part adopts methanol ultrasonic extraction and is developed by toluene-ethyl acetate-formic acid-methanol system, which can simultaneously identify the alpinia oxyphylla, wolfberry and fenxinmu in the refined flat peach pill; the second part is based on the methanol ultrasonic extraction, and hydrochloric acid is added to heat and reflux extraction, shaken and evaporated, and then ethanol is redissolved and developed by petroleum ether-ethyl acetate system, which can identify the ophiopogon in the refined flat peach pill. That is, only two sample preparation methods are required, and one thin layer plate can simply, quickly and effectively identify the four medicinal materials in the refined flat peach pill, and the color spots are clear, without tailing, without cross-linking and good specificity.

The invention relates to the technical field of thin-layer chromatography detection, and discloses a thin-layer chromatography separation method for amino polyether and a matrix in a fluorine-18 labeled radiopharmaceutical, and the adopted developing solvent is acetonitrile and ammonia water, and the volume ratio of the acetonitrile to the ammonia water is 90: 10-85: 15. According to the method, amino polyether and matrix components are separated by adopting a developing solvent with a specific volume ratio, and particularly, sodium ascorbate in the amino polyether and the matrix is separated.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to stasis-removing and pain-relieving pills and a quality detection method thereof. The stasis-removing and pain-relieving pill is prepared by taking radix paeoniae alba, rhizoma corydalis, radix paeoniae rubra, angelica sinensis, ligusticum wallichii, rhizoma cyperi, rhizoma zingiberis, fennel and astragalus membranaceus as raw materials and taking 10% starch slurry as an adhesive. The quality detection method of the stasis-removing and pain-relieving pill comprises general inspection, microscopic identification and thin-layer chromatography identification. And high performance liquid chromatography content determination. According to the present invention, by researching the preparation process and the quality standard of the stasis removing and pain relieving pill, the new thought and the new method are provided for the development of the traditional Chinese medicine preparation, the excellent choice is hopefully provided for the common gynecologic symptoms such as irregular menstruation, dysmenorrhea, postpartum blood stasis abdominal pain and the like, and the pain caused by the illness is relieved.

The invention discloses a method for detecting glyceride and fatty acid composition ratio in mixed fatty acid glyceride by thin-layer chromatography-gas chromatography coupling, and the method mainly comprises the following steps: 1) internal standard solution is prepared; 2) mixed fatty acid glyceride need testing solution is prepared; 3) thin-layer chromatography is used to separate mixed fatty acid glyceride; 4) fatty acid methyl ester is prepared; 5) gas chromatography is used to detect, and the fatty acid composition of monoglyceride, diglyceride and triglyceride and the ratio of each fatty acid are determined respectively; and 6) the sum of the fatty acids of each monoglyceride, diglyceride and triglyceride obtained after data analysis in step 5) is divided by the fatty acid sum of monoglyceride, diglyceride and triglyceride, and the composition ratio of each monoglyceride, diglyceride and triglyceride in mixed fatty acid glyceride is obtained. The method of the present invention is simple and efficient to operate, and is quantitatively accurate, and methyl esterification reaction conditions are mild, equipment is simple, and no special equipment is required.

Disclosed is a thin-layer chromatography identification method for Rhizoma dioscoreae hypoglaucae formula granules. The method comprises: taking a sample to be subjected to detection, and dissolving s

The invention discloses a preparation method of a dialkynyl silane compound, which belongs to the technical field of organic synthesis and comprises the following steps: sequentially adding a reducing agent, an organic solvent, alkynyl bromide and chlorosilane into a reaction container, and reacting at 20-60 DEG C for 6-36 hours to obtain a mixture A; wherein R1 is allyl, alkyl or aryl; wherein R2 is allyl, alkyl or aryl; wherein R3 is an alkenyl group, an alkyl group, an alkoxy group or a silicon group; wherein R is halogen, alkyl, aryl, alkoxy, ester group, cyano or carbonyl; diluting the mixture A with ethyl acetate, adding silica gel powder, and performing vacuum concentration to remove the reaction solvent to obtain a mixture B; and separating the mixture B through thin-layer chromatography or column chromatography to obtain the target product dialkynyl silane compound. According to the method, dialkynyl silane substituted by different alkynyl groups can be obtained at the same time, the chemical selectivity is good, and dialkynyl silane which is not easy to obtain by other methods can be synthesized.

This invention belongs to the field of traditional Chinese medicine detection technology, specifically relating to a multi-component thin-layer chromatography (TLC) identification method for pediatric runtong syrup. This invention establishes specific TLC identification processes for roasted radish seed, immature bitter orange, hemp seed, and scrophularia root, optimizing the sample pretreatment, developing solvent ratio, spotting volume, and colorimetric inspection conditions for each component. After specificity and robustness studies, the method demonstrates good stability under varying temperature, humidity, TLC plate type, and spotting volume ranges, exhibiting no negative interference and clear spots, enabling accurate multi-component identification. This invention is the first to establish a TLC identification method for these four medicinal materials in pediatric runtong syrup, providing a scientific and reliable technical means for the quality control of this compound preparation and filling a gap in existing technology.

The present invention provides a thin-layer chromatography (TLC) identification method for Amomum villosum formula granules, comprising the steps of preparing a test solution, preparing a control medicinal material solution, and performing TLC identification. The test solution and the control medicinal material solution are prepared using a simple and rapid preparation method, and are spotted on the same TLC plate. After development, the TLC plate is inspected under ultraviolet light to obtain a multi-information TLC of the Amomum villosum formula granules. This method uses the control medicinal material as a reference and flavonoids as the main identification component. It has high specificity and can distinguish Amomum villosum of different origins (Amomum villosum and Amomum villosum var. amomi) and identify mixed and counterfeit Amomum villosum (Amomum villosum var. longifolia).

The invention discloses a flavonoid enantiomer compound extracted from Epimedium sagittatum and its preparation method and application. The preparation method comprises the following steps: crushing Epimedium sagittatum to prepare a paste, suspending the paste with water, extracting the paste with petroleum ether, dichloromethane, ethyl acetate and n-butanol respectively to obtain extracts of each part, performing gradient elution separation by silica gel column chromatography, combining similar parts according to the color development results of thin layer chromatography (TLC) to obtain multiple polar segments; chromatographing the target polar segment and performing gradient elution to obtain multiple components, and then combining similar components by thin layer chromatography (TLC) spot plate absorption to obtain multiple sub-components; eluting and purifying the target sub-components, collecting fractions with different retention times, and drying to obtain four compounds respectively. The four compounds have a significant inhibitory effect on the cell viability of human breast cancer MCF-7 cells and have anti-breast cancer activity. The four compounds can be used in the preparation of drugs for treating breast cancer, with huge social and economic benefits.

The invention relates to the technical field of cistanche deserticola detection, in particular to a method for simultaneously and rapidly detecting four phenylethanoid glycosides in cistanche deserticola, which comprises the following steps: preparing a plurality of parts of mixed reference substance solutions containing four phenylethanoid glycosides with known concentrations; preparing a to-be-detected cistanche sample into a test solution; spotting the mixed reference substance solution and the test solution on the same thin-layer plate, carrying out thin-layer chromatography development by adopting a developing solvent, and volatilizing the solvent; placing the thin-layer plate after the solvent is volatilized under ultraviolet light to collect spot images, and processing the images by adopting image analysis software to obtain integral gray values of all phenylethanoid glycoside spots; respectively drawing a standard curve by taking the known concentrations of the four phenylethanoid glycosides in each mixed reference substance solution as horizontal coordinates and the corresponding integral gray values as vertical coordinates, establishing a linear regression equation, and calculating the contents of the four phenylethanoid glycosides in the cistanche deserticola sample to be detected; the invention provides a rapid and accurate method for determining the content of four phenylethanoid glycosides in cistanche.

The invention discloses a traditional Chinese medicine compound composition for treating insomnia and a quality detection method thereof. The traditional Chinese medicine compound composition is prepared from cassia twig, dried ginger, schisandra chinensis, raw Chinese yam, cortex moutan, inula flower, lophatherum gracile, ginger processed pinellia, raw coix seed, raw oyster shell and cortex albiziae. According to the pathogenesis characteristics of insomnia and the principle of syndrome differentiation and treatment of traditional Chinese medicine, the optimal raw material composition and dosage part ratio are screened out through a large number of experiments, the prepared traditional Chinese medicine compound can nourish the liver, calm the heart and regulate the spirit, can improve clinical symptoms such as short-term insomnia disorder, dizziness, palpitation, sweating and restlessness, and has a wide application prospect. The clinical curative effect of treating insomnia is obviously improved. Thin-layer chromatography qualitative identification and high performance liquid chromatography are adopted for quantitative detection, the content of active ingredients of the rhizoma zingiberis, the Chinese yam, the moutan bark and other medicinal materials in the formula is comprehensively and objectively detected and analyzed, and methodological detection shows that the high performance liquid chromatography provided by the invention is high in precision and good in repeatability and stability.

The invention discloses a tissue regeneration promoting jade red gel and a quality detection method thereof.The tissue regeneration promoting jade red gel is characterized in that on the basis of a single-factor test, the dosage of carbomer 940, medicine and triethanolamine is used as influence factors, and the optimal proportion of all components of the gel is determined through an orthogonal test method: the dosage of carbomer 940 is 1%, the medicine loading capacity is 11%, and the dosage of triethanolamine is 1%; the results of accelerated stability and long-term stability tests show that the preparation has good stability in the investigation period. According to the method, the optimal thin-layer chromatography development condition is screened out, the radix angelicae sinensis, the radix arnebiae seu lithospermi and the sangusis draconis in the tissue regeneration promoting jade red gel are subjected to qualitative identification research, and chromatographic conditions such as the optimal flowability composition and a gradient elution mode are screened out through a large number of experiments; according to the present invention, with the high performance liquid chromatography method, the contents of the effective components such as ferulic acid, liquiritin, imperatorin, ligustilide, isoimperatorin and the like can be simultaneously determined, and the methodological detection results show that the high performance liquid chromatography method has characteristics of high precision, high accuracy, good repeatability and good stability.

This invention provides a sample pretreatment method and identification method for identifying ginseng polysaccharides in compound fluorouracil oral solution, belonging to the field of pharmaceutical detection and analysis technology. The sample pretreatment method of this invention includes: centrifuging the compound fluorouracil oral solution and taking the clear solution; adding silica gel to the clear solution, mixing, and removing the solvent to obtain a solid mixture; adding ethanol solution or anhydrous ethanol to the obtained solid mixture, heating and refluxing, discarding the ethanol solution, and obtaining the residue; adding water to the obtained residue, sequentially extracting and centrifuging, taking the supernatant obtained by centrifugation, removing the solvent from the supernatant, and then redissolving it with water; the identification method of this invention is thin-layer chromatography analysis, and the developing solvent includes the following components in parts by volume: 6.8-9.2 parts of n-propanol, 1.76-2.24 parts of water, 0.75-1.24 parts of ethyl acetate, and 0.75-1.24 parts of glacial acetic acid. The sample pretreatment method and identification method for identifying ginseng polysaccharides in compound fluorouracil oral solution of this invention have high specificity.

The present application relates to the technical field of pharmaceutical analysis, and in particular to a thin-layer identification method for Qingxiangteng formula granules and standard decoction, which comprises the following steps: subjecting test sample solution and control medicinal material solution to thin-layer chromatography analysis, and comparing the obtained chromatograms, wherein the developing agent is a mixed solution of chloroform, methanol and formic acid in a volume ratio of (5-7):(0.5-2):(0.4-0.6). The present application is simple to operate, free of negative interference, and clear in color development, and is specific and durable, thus being an improvement on the existing Qingxiangteng preparation identification method.

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a thin-layer chromatography identification method for 8 medicines in 11 medicines in the whole formula of Qingjin phlegm-reducing decoction. The core of the method is that three different test solutions are adopted to realize the identification of eight medicines, namely scutellaria baicalensis, rhizoma anemarrhenae, exocarpium citri rubrum, fructus gardeniae, liquorice, thunberg fritillary bulb, platycodon grandiflorum and radix ophiopogonis. Wherein the first test solution is prepared by extracting water-saturated n-butyl alcohol, and can be simultaneously used for identifying scutellaria baicalensis, rhizoma anemarrhenae, exocarpium citri rubrum, fructus gardeniae and liquorice; the second solution is subjected to ammoniation and dichloromethane extraction to be used for thunberg fritillary bulb identification; the third solution is extracted by dichloromethane after being hydrolyzed by hydrochloric acid, and platycodon grandiflorum and radix ophiopogonis can be identified at the same time. According to the method, by optimizing a test sample preparation and development system, sharing of test samples of multiple medicines and synchronous identification are achieved, the detection efficiency is remarkably improved, the method has the advantages of being easy and convenient to operate, clear in spot, good in separation degree, high in specificity, negative, free of interference and the like, and a reliable basis is provided for quality control of the Qingjin phlegm reducing decoction.

The invention discloses a product collecting device and a control method thereof, and relates to the technical field of thin layer chromatography, the product collecting device comprises a base, a first driving module, a bottom plate, a second driving module and a collecting module, the base has a first direction, a second direction and a third direction; the first driving module is arranged on the base, and a driving part of the first driving module can linearly move along a first direction relative to the base; the bottom plate is connected with the driving part of the first driving module and is used for placing a silica gel plate; the second driving module is arranged on the base, and a driving part of the second driving module can move in the plane where the second direction and the third direction are located relative to the base; the collecting module is connected with the driving part of the second driving module, the collecting module comprises a scraping assembly and a suction assembly, the scraping assembly is used for scraping the product from the silica gel plate, and the suction assembly is used for collecting powder of the product. The product collecting device provided by the invention can realize the automation of thin-layer chromatography product recovery, and reduces dust flying.

This invention belongs to the technical field of qualitative determination of ephedra, licorice, and bitter almond in traditional Chinese medicine compound preparations. This invention discloses a method for simultaneously identifying ephedra, licorice, and bitter almond in Ma Xing Shi Gan oral liquid. The test sample is placed in a Soxhlet extractor and treated with reagents of different properties to prepare a fractional extraction solution. Ephedra and licorice reference materials are then processed to obtain a reference material solution. Amygdalin reference standard is prepared by adding methanol to prepare a reference solution. The above test sample solution, reference material solution, and reference solution are used in a thin-layer chromatography system to simultaneously identify ephedra, licorice, and bitter almond. The results show that the chromatographic spots are well separated and clear, with no interference from the negative control. This method has simple pretreatment, is environmentally friendly, has high sensitivity, and its repeatability and robustness meet the requirements of the Chinese Veterinary Pharmacopoeia, thus enabling better quality control of Ma Xing Shi Gan oral liquid.

The invention discloses a rapid detection method of isobutyramido thiazolyl resorcinol in cosmetics, and belongs to the technical field of safety detection and analysis of cosmetics. Thin-layer chromatography is taken as a main line, separation, 254nm and 365nm dual-band ultraviolet observation, 105 DEG C thermal activation enhancement and three-channel color development recheck of ferric trichloride, vanillin-sulfuric acid and phosphotungstic acid are sequentially completed, and the detection result is accurate. The method comprises the following steps: acquiring multilayer evidences of physical separation, optical response and chemical reaction, establishing a standardized ratio shift value according to upper and lower interior labels and a solvent front edge, extracting indexes such as brightness and color difference, dispersing displacement and intensity signals into five-bit bar codes, realizing fault-tolerant matching by using a Hamming distance, outputting positive, confirmation or negative conclusions, and determining the accuracy of the result. And generating a report containing the original image and the parameters. The method is oriented to complex matrixes such as emulsion, face cream, essence, mask liquid, lipstick and eye shadow, by means of multi-mechanism cross validation and bar code type fusion interpretation, the false positive and false negative risks are remarkably reduced, and the result consistency and the rechecking efficiency are improved.

The application discloses a method for detecting clenbuterol based on thin layer chromatography and surface enhanced resonance Raman spectroscopy, and belongs to the technical field of food detection. The method comprises the following steps: (1) adopting thin layer chromatography to preliminarily separate the clenbuterol component from the matrix in the pig liver sample, and confirming the clenbuterol component in the pig liver sample under the ultraviolet lamp of 254 nm; (2) adding aromatic diazonium salt solution to the same Rf value of the clenbuterol component reference sample spot to generate azo compounds, and then adding a surface enhancer silver sol; (3) in-situ detecting the surface enhanced resonance Raman spectrum of the corresponding azo compound component spot, and if the surface enhanced resonance Raman spectrum of the pig liver sample solution at the same Rf value is the same as that of the reference sample solution, it is determined that the pig liver sample contains the clenbuterol component. The application has high specificity for detecting clenbuterol and salbutamol, high sensitivity, fast analysis speed, and is beneficial to fast detection of grass-roots units. The application is suitable for detecting clenbuterol.

The invention provides a method for identifying and detecting a phlegm stasis and arthralgia dredging prescription. The method comprises three modules of construction of a characteristic chromatogram of a medicinal material and optional thin-layer chromatography identification and effective component content determination. The construction of the characteristic spectrum adopts a diethyl ether-ethyl acetate two-stage segmented extraction UPLC analysis strategy, so that the interference of complex components is overcome, and the overall chemical characteristics of the compound can be comprehensively represented; thin-layer chromatography identification provides a specific identification method for medicines such as coptis chinensis, curcuma wenyujin and vinegar-processed rhizoma corydalis; and the content determination adopts a high performance liquid chromatography to detect the effective components chlorogenic acid, salvianolic acid B, glycyrrhizic acid and berberine hydrochloride. According to the invention, a multi-dimensional and configurable quality control system is constructed, the comprehensive quality control of the phlegm stasis and arthralgia dredging prescription from qualitative and overall characterization to quantitative analysis is realized, the specificity is strong, and the result is reliable.

The present invention relates to the field of thin layer identification of Chinese medicinal materials in traditional Chinese medicine preparations, and in particular to a method for thin layer identification of Chinese herbal medicines and Chinese herbal medicines. It comprises the following steps: 1) crushing Chinese herbal medicines, soaking them in ethanol, ultrasonic extraction, collecting the filtrate, concentrating it, extracting it with petroleum ether, and evaporating it to obtain a residue; 2) dissolving the residue with petroleum ether and eluting it on a silica gel column three times, eluting it with petroleum ether for the first time, eluting it with petroleum ether-ethyl acetate with a volume ratio of 5 to 15:1 for the second time, and eluting it with petroleum ether-ethyl acetate with a volume ratio of 1 to 5:1 for the third time, collecting the third eluate, evaporating it to obtain a test sample; 3) spotting the test sample and the control medicinal material on a silica gel G thin layer plate, and performing thin layer chromatography identification with n-hexane-ethyl acetate as a developing agent. Chinese herbal medicines are compound preparations, and there is mutual influence between the components. The test solution prepared by the present invention can effectively purify the sample, reduce the interference of other components, and improve the accuracy of detection. At the same time, in the thin layer identification chromatogram, the developing agent is optimized, the color spots are clear, and it has strong specificity. The invention is used for quality control of Zhonghua Dieda Pills, ensuring the quality of the medicine and the safety, effectiveness and reliability of clinical medication.