351 results about "Thin layer chromatographic" patented technology

The invention discloses a rapid qualitative and quantitative detection method for quantitative analysis of multiple components by single marker of rhodiola rosea, astragalus membranaceus and safflower capsules, and belongs to a detection and analysis technology. According to the method, three traditional Chinese medicines, namely rhodiola rosea, astragalus membranaceus and safflower carthamus, are identified on a thin-layer plate by adopting a thin-layer chromatography method and a same test solution, the pretreatment of a sample and a reference medicinal material only needs 3 hours, 265 mL of a solvent is adopted, spots are clear, and the method is rapid; according to the established single-detection double-evaluation method for salidroside and calycosin-7-glucoside, only an ultraviolet detector is needed, detection is carried out under the wavelength of 270 nm, chromatographic peaks do not interfere with one another, single-detection double-evaluation is achieved, the efficiency is high, and the cost is low. The invention breaks through a traditional identification mode that N traditional Chinese medicine varieties are identified, N test solutions and N thin-layer plates are prepared, N times of development are needed, and N medicinal materials are identified, and establishes a rapid thin-layer identification method for three traditional Chinese medicines on one thin-layer plate by adopting the same test solution and each reference medicinal material supernatant subjected to methanol ultrasonic treatment.

The invention discloses a tea oil adulteration detection method based on thin-layer chromatography and image analysis, and belongs to the technical field of tea oil adulteration detection.The tea oil adulteration detection method comprises the steps that firstly, tea oil with different adulteration concentrations is analyzed based on thin-layer chromatography, oil sample images are obtained, and therefore an image recognition database is established; the adulterated tea oil lipid and lipid adjunct with different concentrations have different color development positions under the action of the composite organic solvent, so that the adulterated tea oil machine identification model is constructed by taking different color development positions as characteristic indexes. And identifying a thin-layer chromatogram of a to-be-detected oil sample by adopting the tea oil machine identification model, and judging the adulteration condition of the tea oil according to an identification result. According to the method, the thin-layer chromatography analysis and the machine algorithm are combined, and the thin-layer chromatogram of the sample to be detected is identified and distinguished by utilizing the machine algorithm, so that the tea oil adulteration result and adulteration concentration can be obtained, and the method is simple, convenient and rapid to operate and easy to popularize and promote.

The preparation method comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone compound extracted from epimedium sagittatum, thereby obtaining the isopentenyl flavone compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and two compounds are respectively obtained, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The preparation method of the flavone enantiomer compound comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the flavone enantiomer compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and four compounds are obtained respectively, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The present invention relates to a digital thin-layer chromatography detection and analysis model, a method for establishing the same, and an application thereof, belonging to the technical field of traditional Chinese medicine quality control. The digital thin-layer chromatography detection and analysis model is established based on the relative migration distance value (RPf) theory, and can be used for digital thin-layer chromatography analysis without physical reference. Under different experimental conditions and for different experimental varieties, the relative standard deviation of the RPf value is the smallest, showing better performance than RRf, and demonstrating the application advantages of the characteristic spot migration distance method in the digital standard of traditional Chinese medicine thin-layer chromatography.

The preparation method of the isopentenyl flavone enantiomer compound comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, respectively extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone enantiomer compound, namely the isopentenyl flavone enantiomer compound. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and four compounds are obtained respectively, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention provides a specific chromatogram construction and content determination method of the radix asparagi and radix ophiopogonis decoction preparation and a thin-layer chromatography identification method of traditional Chinese medicine flavor components in the radix asparagi and radix ophiopogonis decoction preparation. The specific chromatogram construction method of the radix asparagi decoction preparation comprises the following steps: step 1, taking mangiferin, quercetin-3-O-glucuronide, baicalin, wogonoside and ammonium glycyrrhizinate reference substances, and adding a solvent to prepare a reference substance solution; step 2, taking an Eupatorium japonicum-Liupu decoction reference sample, and adding a solvent to prepare a test solution; and 3, respectively detecting the reference substance solution and the test solution by using high performance liquid chromatography, and by taking the characteristic spectrum of the reference substance solution as a reference spectrum, selecting a common peak from the characteristic spectrum of the test solution to construct the characteristic spectrum of the radix asparagi decoction preparation.

The invention belongs to the technical field of quality control of traditional Chinese medicines, and particularly relates to a thin-layer chromatography identification method for simultaneously identifying various components of angelica sinensis potion. According to the thin-layer chromatography identification method, firstly, ethyl acetate-n-butyl alcohol is adopted for shaking extraction, ethyl acetate-n-butyl alcohol liquid is taken and evaporated to dryness, water-soluble components which have large interference on identification are removed, a methylbenzene-ethyl acetate-formic acid system is developed, and then angelica sinensis, ligusticum wallichii and ginger in the angelica sinensis potion can be identified at the same time; and further removing acidic tailing components which have great interference on identification from the test sample through alumina column chromatography, and developing by using an ethyl acetate-formic acid-glacial acetic acid-water system, so that white paeony root, divaricate saposhnikovia root, liquorice and polygonum multiflorum in the Chinese angelica potion can be identified at the same time. The seven medicinal materials in the angelica sinensis potion can be simply, rapidly and effectively identified only by two thin-layer plates, and the method has the advantages of clear color spots, no tailing, no crossing and good specificity.

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention discloses a compound donkey-hide gelatin pulp thin-layer chromatography fingerprint construction and combination evaluation analysis method, and belongs to the technical field of traditional Chinese medicine quality control. The compound colla corii asini pulp thin-layer chromatography is a thin-layer chromatography fingerprint spectrum of the compound colla corii asini pulp or an intermediate in a process preparation process; the construction of the thin-layer chromatography fingerprint spectrum is based on the color of a thin-layer chromatography sample strip image and the gray scale of spots, and a pixel-intensity matrix and a ratio shift value-gray scale matrix are formed to comprehensively reflect the thin-layer chromatography characteristics of the sample; according to the combined evaluation method of the thin-layer chromatography fingerprint spectrum, a method of combining machine learning with information entropy and entropy is adopted, ginsenoside Rg1, codonopsis pilosula alkynol and other components are screened and identified as key components influencing the compound donkey-hide gelatin pulp and transmission, and the method has the characteristics of accuracy and high efficiency, is wide in application prospect and can provide a thought for establishment of quality standards of traditional Chinese medicine compounds.

Disclosed is a thin-layer chromatography identification method for Rhizoma dioscoreae hypoglaucae formula granules. The method comprises: taking a sample to be subjected to detection, and dissolving same in a solvent to obtain a test solution, wherein the sample to be subjected to detection involves Smilax glaucochina formula granules or Smilax ferox formula granules; taking resveratrol as a control, and adding a solvent to dissolve same to obtain a control solution; taking a Smilax ferox control medicinal material, and dissolving same in a solvent to obtain a control medicinal material solution; and subjecting the test solution, the control solution and the control medicinal material solution to thin-layer chromatography detection, wherein a thin-layer plate is a silica gel G thin-layer plate, and a developing solvent is toluene-ethyl acetate-formic acid. The sample is placed under an ultraviolet lamp for inspection, and if in the chromatogram of the test sample there are blue fluorescent spots at the position of an Rf value of 0.829 ± 10%, the sample is Smilax ferox, and if there are no blue fluorescent spots at the position of the Rf value of 0.829 ± 10%, the sample is Smilax glaucochina.

The invention discloses a thin-layer chromatography identification method for cinnamon and ginger in vitality-preserving granules, and belongs to the technical field of pharmaceutical analysis. Aiming at the problems of step-by-step detection, serious cinnamic acid tailing and dependence on toxic solvents in the existing method, the method comprises the following steps: dissolving vitality-preserving particles in water, carrying out ultrasonic treatment, extracting filtrate by using absolute ether, merging extract liquor, evaporating to dryness, and dissolving by adding ethyl acetate to obtain a test solution; preparing a cinnamon reference medicinal material solution and a cinnamic acid and 6-gingerol reference substance solution; a silica gel GF254 thin-layer plate is adopted, petroleum ether-normal hexane-ethyl formate-formic acid is used as a developing solvent for developing, and inspection is conducted under the ultraviolet light of 254 nm and the ultraviolet light of 365 nm respectively. Cinnamon and ginger can be synchronously identified, spots are clear and free of trailing, no toxic solvent exists, durability is high, cost is low, and an accurate and reliable basis can be provided for quality control of the vitality-preserving granules.

The invention belongs to the technical field of quality control of traditional Chinese medicines, and particularly relates to a specific chromatogram construction method and a quality control method of radix astragali and cassia twig five-material decoction, and the quality control method comprises a specific chromatogram identification method and a thin-layer chromatography identification method. The UPLC is used for construction and identification of the characteristic spectrum, and the method is short in detection time, high in specificity, rapid, efficient and low in cost. According to the specific chromatogram, 17 common characteristic peaks are stipulated, 12 characteristic peaks are identified, the identification rate is high, and analysis and identification of the material basis of the radix astragali and cassia twig five-material decoction are facilitated. According to the thin-layer chromatography identification method disclosed by the invention, a novel thin-layer identification method for identifying two medicines (astragalus membranaceus and radix paeoniae alba) in a compound by quantitative analysis of multi-components by single marker is successfully created, the detection conditions are simple, rapid and efficient, the specificity is high, the cost is relatively low, and the practicability is relatively high.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a thin-layer chromatography identification method for the flavor of whole medicines in Houshuning tablets. The thin-layer chromatography identification method for the flavor of the whole medicine in the Houshuning tablet, provided by the invention, comprises the following steps: S1, preparing a test solution of the Houshuning tablet; s2, preparing a reference medicinal material solution of spreading hedyotis herb, common andrographis herb and screwtree root; s3, adopting a polyamide thin-layer plate, taking absolute ethyl alcohol-acetic acid-water as a developing solvent, spraying an aluminum trichloride-ethanol solution for inspection, and performing thin-layer chromatography identification. According to the method disclosed by the invention, the method exclusively used for identifying the flavonoid components of the three medicinal materials in the prescription of the Houshuning tablet is established, the improvement of quality control of the Houshuning tablet is promoted, and meanwhile, the method can be used as a reference for researching the quality of the flavonoid components of the single medicinal materials such as oldenlandia diffusa, andrographis paniculata and helicteres angustifolia; and the blank of oldenlandia diffusa, andrographis paniculata and helicteres angustifolia in the aspect of flavone thin-layer chromatography identification is filled.

The present invention provides a thin-layer identification method for pinecone formula granules, comprising: A) taking a test sample, and after pretreatment, obtaining a test solution; taking a pinosylvin monomethyl ether reference substance, and after pretreatment, obtaining a reference solution; B) taking a reference medicinal material of pinecone, and after pretreatment, obtaining a reference medicinal material solution; C) performing thin-layer chromatography detection on the test solution, the reference solution and the reference medicinal material solution, wherein the thin-layer plate is a silica gel G thin-layer plate; the developing agent is toluene-ethyl acetate-glacial acetic acid; D) examining, in the test chromatogram, if spots of the same color appear at the positions corresponding to the reference medicinal material and the reference substance, it is pinecone formula granules. The thin-layer identification chromatographic method for pinecone formula granules established by the present invention uses, as the identification points, the spots of the same color that appear at the positions corresponding to the reference medicinal material and the reference substance by observing the thin-layer chromatogram, so as to quickly and effectively identify pinecone formula granules.

The invention relates to the field of modernization of traditional Chinese medicines, in particular to a method for identifying a Dayuan liquid preparation. The invention respectively provides thin-layer chromatography identification methods for pogostemon cablin, bupleurum chinense and glabrous sarcandra herb in the daoyuan liquid preparation, the thin-layer chromatography identification methods for the three components are good in specificity, repeatability and stability, and effective quality control of the daoyuan liquid preparation can be realized.

The application discloses a kind of gardenia and its processed product identification method, comprising: step one, respectively take equal amount of gardenia sample powder, gardenia processed product sample powder;Step two, the above-mentioned powder is respectively soaked with ethanol solution, then ultrasonic extraction is carried out, filtration, filter liquor is evaporated to dryness, obtain gardenia sample crude extract, gardenia processed product sample crude extract;Step three, the above-mentioned crude extract is respectively dissolved with water, then sequentially extracted with petroleum ether, ethyl acetate twice, and the two extraction liquids are combined and evaporated to dryness, obtain extract, the extract is dissolved with methanol, obtain gardenia sample test solution, gardenia processed product sample test solution;Step four, the above-mentioned test solution is respectively spotted on the same silica gel thin layer plate, with developing agent development, dry, with color developing agent color development, and under ultraviolet lamp, view thin layer chromatogram, according to the number, position, color of fluorescence spot on thin layer chromatogram, identify sample variety.

This invention discloses a quality control method for an anti-snoring oral liquid and its application, belonging to the field of traditional Chinese medicine quality testing technology. The method includes thin-layer chromatography identification of Gleditsia sinensis thorns, Scrophularia ningpoensis, and Glycyrrhiza uralensis, as well as high-performance liquid chromatography (HPLC) determination of hesperidin content. By optimizing pretreatment, developing solvent, and colorimetric conditions, this invention achieves efficient and specific identification and quantification of multiple components in the compound preparation. The method is highly specific, produces clear spots, has good separation, and is tolerant to a wide temperature and humidity range (2-40℃, 20%-75%RH), requiring no strict environmental control, significantly improving detection efficiency and providing a reliable basis for establishing quality standards for this preparation.

The invention relates to a method for detecting a clearing agent main agent in gasoline, and the method comprises the following steps: carrying out thin-layer chromatography detection on a to-be-detected sample, and determining the composition of the clearing agent main agent in the concentrated to-be-detected sample according to color spot distribution on an obtained thin-layer chromatography plate, wherein the clearing agent main agent comprises one or more of a polyether amine clearing agent, a polyisobutene amine clearing agent and a Mannich base clearing agent; the thin-layer chromatography detection sequentially comprises developing and developing treatment; a developing agent for developing comprises one or more of a developing agent I, a developing agent II and a developing agent III; the developing solvent I is methanol, and the developing solvent II and the developing solvent III comprise one or more of alcohol with the carbon atom number of 1-6, aliphatic hydrocarbon with the carbon atom number of 5-12 and cycloalkane with the carbon atom number of 5-12. According to the method provided by the invention, whether the clearing agent main agent is added into the sample to be detected or not can be rapidly determined, and the type of the clearing agent main agent can be rapidly and accurately detected.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

The invention discloses a quality detection method for Blumea Balsamifera medicinal material, comprising the use of thin layer chromatography to identify Blumea Balsamifera medicinal material, mainly comprising: taking a test solution and a reference solution of Blumea Balsamifera medicinal material, spotting them on the same silica gel thin layer plate, using petroleum ether-ethyl acetate-chloroform with a volume ratio of 11:1:3 as a developing agent, spraying with a phosphomolybdic acid test solution with a mass content of 5%, and heating until the spots are clearly colored. The invention has the characteristics of accurately identifying the authenticity of Blumea Balsamifera medicinal material.

The present invention discloses a thin layer chromatography identification method for simultaneously identifying multiple components in a refined flat peach pill preparation and its application, which belongs to the technical field of quality control of traditional Chinese medicine. The thin layer chromatography identification method of the present invention is divided into two parts. The first part adopts methanol ultrasonic extraction and is developed by toluene-ethyl acetate-formic acid-methanol system, which can simultaneously identify the alpinia oxyphylla, wolfberry and fenxinmu in the refined flat peach pill; the second part is based on the methanol ultrasonic extraction, and hydrochloric acid is added to heat and reflux extraction, shaken and evaporated, and then ethanol is redissolved and developed by petroleum ether-ethyl acetate system, which can identify the ophiopogon in the refined flat peach pill. That is, only two sample preparation methods are required, and one thin layer plate can simply, quickly and effectively identify the four medicinal materials in the refined flat peach pill, and the color spots are clear, without tailing, without cross-linking and good specificity.

The invention discloses a detection method of a medicine for treating apoplexy sequela, and relates to the technical field of traditional Chinese medicinal material detection. The capsule disclosed by the invention comprises the following components: ginseng, deer-horn glue, tortoise shell, fructus lycii, turtle shell, leech, ligusticum wallichii, rheum officinale, hawthorn, earthworm, scorpio, bungarus parvus, gastrodia elata, stiff silkworm and borneol. During identification, microscopic identification and thin-layer chromatography identification are adopted, the gastrodia elata and the hawthorn are identified by the thin-layer chromatography, and the microscopic identification is used for identifying a capsule finished product; the aims of accurately detecting the ginseng and snake hemiplegia capsule and reducing the large influence on the detection result due to the change of different temperature and humidity in the environment are achieved.

The invention relates to the technical field of thin-layer chromatography detection, and discloses a thin-layer chromatography separation method for amino polyether and a matrix in a fluorine-18 labeled radiopharmaceutical, and the adopted developing solvent is acetonitrile and ammonia water, and the volume ratio of the acetonitrile to the ammonia water is 90: 10-85: 15. According to the method, amino polyether and matrix components are separated by adopting a developing solvent with a specific volume ratio, and particularly, sodium ascorbate in the amino polyether and the matrix is separated.

The invention discloses a longan leaf granule which is prepared by the following steps: adding water into longan leaves, heating, extracting, filtering, concentrating, drying, crushing to obtain dry paste powder, adding a diluent into the dry paste powder, uniformly mixing, adding a wetting agent, granulating, and drying to obtain the longan leaf granule. The forming rate, the dissolution rate, the moisture absorption rate, the fluidity and the like of the granules all meet the requirements, and the granules are good in stability, suitable for industrial batch production, capable of treating diabetes, capable of meeting the requirements of doctors for treatment based on syndrome differentiation and increase and decrease along with symptoms and convenient to carry and take by patients. Thin-layer chromatography is adopted for qualitative identification of the longan leaf granules, high performance liquid chromatography is adopted for content determination of quercetin, quercitrin and kaempferol in the longan leaf granules, operation is easy, convenient and accurate, repeatability is good, and a scientific basis is provided for quality evaluation and control of the longan leaf granules.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to stasis-removing and pain-relieving pills and a quality detection method thereof. The stasis-removing and pain-relieving pill is prepared by taking radix paeoniae alba, rhizoma corydalis, radix paeoniae rubra, angelica sinensis, ligusticum wallichii, rhizoma cyperi, rhizoma zingiberis, fennel and astragalus membranaceus as raw materials and taking 10% starch slurry as an adhesive. The quality detection method of the stasis-removing and pain-relieving pill comprises general inspection, microscopic identification and thin-layer chromatography identification. And high performance liquid chromatography content determination. According to the present invention, by researching the preparation process and the quality standard of the stasis removing and pain relieving pill, the new thought and the new method are provided for the development of the traditional Chinese medicine preparation, the excellent choice is hopefully provided for the common gynecologic symptoms such as irregular menstruation, dysmenorrhea, postpartum blood stasis abdominal pain and the like, and the pain caused by the illness is relieved.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly discloses a thin-layer chromatography method for identifying radix angelicae lateralis preparata components in rhizoma coptidis paste and application of the thin-layer chromatography method. The thin-layer chromatography method provided by the invention comprises the following steps: preparing a test solution: adding methanol water into coptis chinensis paste, heating and melting, and then refluxing and stirring; storing at 0 + / -5 DEG C; then, dichloromethane-n-butyl alcohol with the volume ratio of (2.5-3.5): 1 is adopted as an eluent, silica gel column filtration is performed to obtain a filtrate, and the filtrate is evaporated to dryness and dissolved to obtain a test solution; preparing a reference solution; and detection: respectively dripping the solutions on the same silica gel G thin-layer plate, putting the silica gel G thin-layer plate into a developing cylinder to be developed by taking ethyl acetate-isopropanol with the volume ratio of (3-5): 1 as a developing solvent, and inspecting under a light source of 360-370nm. The thin-layer chromatography method provided by the invention can be used for rapidly and accurately identifying the radix angelicae lateralis preparata component in the rhizoma coptidis paste, and a theoretical basis is provided for quality control of the rhizoma coptidis paste.

Bottom-Interface Thin Layer Chromatography Chamber

The present invention discloses a preparation method of thiazolidine-4-thione derivatives, which comprises the following steps: (1) mixing an isothiocyanate or its derivative, a sulfur ylide, an additive and an organic solvent, and reacting at 20-90 °C for 12-48 h under an air atmosphere; (2) diluting the material obtained in step (1) with ethyl acetate and washing with water, and separating to obtain an organic phase; (3) subjecting the above organic phase to drying, filtration, concentration and column chromatography or thin-layer chromatography in sequence to obtain thiazolidine-4-thione derivatives. The present invention can construct tetra-substituted thiazolidine-4-thione derivatives, can efficiently synthesize thiazolidine-4-thione derivatives containing trifluoromethyl, the raw materials used are easily available, the yield is high, the reaction conditions are mild, the reaction time is short, the substrate range is wide, the reaction specificity is strong, and the post-treatment is simple.

The invention discloses a method for detecting glyceride and fatty acid composition ratio in mixed fatty acid glyceride by thin-layer chromatography-gas chromatography coupling, and the method mainly comprises the following steps: 1) internal standard solution is prepared; 2) mixed fatty acid glyceride need testing solution is prepared; 3) thin-layer chromatography is used to separate mixed fatty acid glyceride; 4) fatty acid methyl ester is prepared; 5) gas chromatography is used to detect, and the fatty acid composition of monoglyceride, diglyceride and triglyceride and the ratio of each fatty acid are determined respectively; and 6) the sum of the fatty acids of each monoglyceride, diglyceride and triglyceride obtained after data analysis in step 5) is divided by the fatty acid sum of monoglyceride, diglyceride and triglyceride, and the composition ratio of each monoglyceride, diglyceride and triglyceride in mixed fatty acid glyceride is obtained. The method of the present invention is simple and efficient to operate, and is quantitatively accurate, and methyl esterification reaction conditions are mild, equipment is simple, and no special equipment is required.