540 results about "Thin layer chromatographic" patented technology

The invention discloses preparation and application of total alkaloids extract and effective components in peganum plant seeds. The peganum plant (comprising harmel, multisectum peganum and peganum nigellastrum) seeds are heated, refluxed and extracted by ethanol, dissolved along with heat, acidulated by added 2 to 10 percent hydrochloric acid, and basified by ammonia water to separate out sediment; and the sediment is dried to obtain the total alkaloids extract mainly containing dehydrogenized peganine and peganine according to a proportion of between 0.18 to 1 and 4.30 to 1, wherein the content of the total alkaloids is more than 50 percent. The total alkaloids extract is separated by chromatography to obtain ten effective monomer components such as the dehydrogenized peganine, the peganine, and the like. Through thin layer chromatography and biological self-developing analysis, the total alkaloids extract and the effective monomer components thereof have the effect of resisting activity of acetylcholine esterase, and can be used for preparing medicament for resisting the activity of the acetylcholine esterase and medicament for treating neurodegenerative diseases.

The use of free, extractable lipids found in bacteria for identification of bacterial species and subspecies is described. Bacteria have been found to differ sufficiently in their extracted lipid compositions to effect identification using thin layer chromatographic techniques. Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei have been distinguished in this manner. Lipopeptides specific to Mycobacterium avium subspecies paratuberculosis, but not to the closely related bacterium Mycobacterium avium subspecies avium have also been used as a basis for bacterial subspecies identification using mass spectrometry and seroreactivity. Mass spectrometric analysis of total bacterial lipids of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei, and mass spectrometric analysis of total bacterial lipids for Mycobacterium avium subspecies paratuberculosis and Mycobacterium avium subspecies avium, without further lipid separation, has shown that species and subspecies of bacteria may be identified using such analysis.

The invention relates to a pharmaceutical composition for treating chronic pharyngitis, in particular to a traditional Chinese medicinal composition for treating chronic pharyngitis, as well as a preparation method and a quality control method thereof. The pharmaceutical composition comprises figwort roots, radix asteris, radix asparagi, cortex moutan radicis, ophiopogon roots and other raw medicinal materials, and is prepared into various preparations suitable for clinical application according to traditional Chinese medicinal routine techniques, including tablets, capsules, orally taken liquid preparations, dropping pills, granules, and the like. The quality control method comprises qualitative identification for thin-layer chromatography, content determination of high performance liquid chromatography and other methods. The pharmaceutical composition has good effects of treating throat dryness, throat itch, irritable cough and other symptoms caused by chronic pharyngitis.

The invention provides a method for detecting a Chinese patent drug containing at least two of white paeony root, ginseng, salvia miltiorrhiza, sweet wormwood, liquorice and angelica sinensis, comprising the steps as follows: preparing a sample solution and a comparison product drug solution, adopting acetonitrile and water as mobile phases, and performing high performance liquid chromatography (HPLC) measurement, wherein the detection wavelength is 201-290nm; and detecting whether a corresponding chromatographic peak exists at a position, which has the same reservation time with the chromatograph of a comparison drug, in the chromatography of a sample. Compared with the traditional thin-layer chromatography identification method, the detection method in the invention has the advantages that the mode of processing a sample containing various drugs in a liquid phase chromatograph is changed, the mixed peak in the liquid phase chromatography except the components to be detected in the Chinese patent drug is successfully eliminated, and results are more accurate and stable.

The invention discloses a quality detection method for a houtou jianweiling Chinese medicament, which comprises the following steps: performing thin layer chromatography identification on rhizoma corydalis; and measuring that the content of paeoniflorin in radix paeoniae alba is not less than 0.40 mg by a high performance liquid chromatography. The method also relates to the following detection steps: a, identifying the thin layer chromatography of rhizoma cyperi; b, identifying the thin layer chromatography of the radix paeoniae alba and liquorice; and c, measuring that the content of adenosine C10H13N5O4 is not less than 0.070 mg by the high performance liquid chromatography. The method can effectively improve the quality standard of the houtou jianweiling Chinese medicament; and the detection method can come up with new related detection indexes, adds detection indexes, strictly controls the product quality, and ensures the efficacies.

A method for enriching and purifying resveratrol from peanut root by macro-porous adsorptive resin is carried out by crushing dried peanut root, extracting by organic solvent, extraction filtering for extract, recovering solvent to obtain first extract, heating by water, dissolving, adding into acetic ether, extracting, regulating pH value to 7.0-11.0, laying aside, extraction filtering, decompressing, concentrating to obtain resveratrol coarse extract, adsorbing by macro-porous adsorptive resin, eluting by alcohol, tracking while inspecting by thin-layer chromatographic method, concentrating while recovering alcohol to obtain half-finished product with content above 35%, coating silica gel by chromatographic column, gradient eluting by different proportion acetic ether and chloroform, tracking while inspecting by thin-layer chromatographic method, de-coloring by active carbon and re-crystallizing to obtain final product with content above 98%. It's fast, cheap and convenient, has high purity and various raw materials.

Ascidian polypeptide and its production are disclosed. The procedure is carried out by immersion depressing at normal temperature by alcohol, recovering alcohol to obtain extract, extracting by organic solvent, concentrating, separation purifying by macroporous resin chromatography, silica-gel chromatography and Sephadex LH20 chromatography, gradient eluting by eluent in proportion of CHCl3: MeOH: H2O=7:3:0.5, and collecting concentrated eluent with thin-layer chromatographic ultraviolet verification Rf=0.5~0.6. The molecular weight of ascidian polypeptide is 711.4. It is simple, has less investment, convenient control for production and various raw materials.

The invention provides a suture-free bilioenteric anastomosis stent manufactured through 3D (three-dimensional) printing. The bilioenteric anastomosis stent comprises a stent pipe and bulging columns bulging out of the outer wall of the stent pipe, and bulging heads are integrally connected at two ends of the stent pipe. The bilioenteric anastomosis stent is made from a degradable biological material through a 3D printing technique. A manufacturing method comprises steps as follows: (1), the diameter of the bile duct of a patient requiring gallbladder-pancreas reconstructive surgery is tested through CT (computed tomography); (2), required 3D printing data are acquired through algorithm conversion and image processing according to a bile duct CT thin-layer chromatographic scanning result, and the bulging columns and the bulging heads are designed on fit contact surfaces; (3), a 3D printer which is most precise currently is used, the biocompatible degradable material is used, the 3D bilioenteric anastomosis stent is printed and manufactured, and the bilioenteric anastomosis stent is applied to suture-free bilioenteric anastomosis. The suture-free bilioenteric anastomosis stent has the benefits of simple structure, convenience in use, remarkable effect, capability of realizing suture-free bilioenteric anastomosis, the risks of anastomotic leakage and stenosis are reduced, and the operation difficulty and risks are greatly reduced.

The invention relates to an intelligent individual exoskeleton device for facture treatment. The intelligent individual exoskeleton device is characterized by comprising an intelligent individual exoskeleton and an installation assisting device. The installation assisting device comprises a steel needle with scale marks, a skeleton reset mechanism and a reference fixing plate. The steel needle with scale marks and the skeleton rest mechanism are installed at a focus position corresponding to the intelligent individual exoskeleton in a matched mode through the reference fixing plate. The device is designed by reserving advantages of internal and external fixation and overcoming defects of internal and external fixation. The exoskeleton is obtained through 3D printing; according to the design of the exoskeleton, a series of technologies of precision needle setting, CT thin-layer chromatographic scanning, computer virtual resetting, biomechanics design and the like are included, and the intelligent individual customized solution for fracture treatment is obtained. The purposes of minimal invasion, precision resetting, firmness, fixation and early recovery of limbs can be achieved.

The invention relates to a quality detection method for chinarue cough particles. In the formula, the chinarue cough particles include following materials: 156g of pericarpium citri reticulatae, 96g of rhizoma acori graminei, 312g of polygonum cuspidatum, 188g of asparagus cochinchinensis, 375g of chinarue, 96g of radix stemonae, 750g of marsdenia tenacissima, 375g of laggera pterodonta, 96g of semen armeniacae amarae, 375g of houttuynia cordata and 188g of cortex mori radicis, and the materials are prepared into particles. On the basis of the original standard, the thin-layer chromatography identification of the chrinarue is added, and the chinaure is one of main ingredients. After the thin-layer indentification of the chinaure is added on the basis of the original standard, the scientific basis is provided for qualitative control of the chinaure in the preparation, the quality detection standard of the chinaure cough particles is perfected, and the scientific basis is provided for the judgment of true and false and quality control of the product. In the method, the test and the operation of samples are simple and convenient, and by the continuous verification test of negative interference and the like, free interfered negativeness, good repeatability, and strong specificity are verified and tested. The quality test standard is perfected, and the product quality control capability is promoted.

The invention discloses a method for detecting the quality of bezoar snake bile bulbus fritilariae liquid which consists of the following raw medicament components in part by weight: 1.0 to 2.0 parts of artificial bezoar, 40 to 60 parts of unibract fritillary bulb, 6.0 to 10 parts of snake bile and 0.03 to 0.05 part of menthol. The method comprises the following steps of chemical identification of the unibract fritillary bulb, thin layer chromatography identification of the artificial bezoar, thin layer chromatography identification of the snake bile, and efficient liquid-phase method content determination of bile acid. The invention also discloses a preparation process and preferential raw material components of the bezoar snake bile bulbus fritilariae liquid. The method for detecting the quality can reflect the preparation quality of the bezoar snake bile bulbus fritilariae liquid from a plurality of aspects so as to ensure the curative effect and the medication safety.

A separation method of swainsonine-producing endophytic fungi in glabrous crazyweed comprises the following steps of: acquiring mycelium of the strain and a broth through a bio-fermentation method, carrying out ultrasonic extraction, quantitatively and qualitatively detecting swainsonine by thin-layer chromatography and gas chromatography technologies after solvent extraction, and identifying swainsonine-producing endophytic fungi through morphological and molecular biology methods. The result shows that one out of six separated strains of endophytic fungi can generate swainsonine and the output is 16.7338 mg/L. After identification, the strain is fusarium proliferatum. In comparison with present strains for the production of swainsonine, swainsonine provided by the invention has high output. The bio-fermentation method for the production of swainsonine doesn't influence the meadow ecological environment. The separation method provided by the invention solves the problem of swainsonine sources and provides strain supports, thus establishing a theoretical foundation for future industrial production of swainsonine. The invention has a wide development prospect.

The invention discloses a method for detecting a medicinal oral preparation for treating irritable bowel syndrome. The medicinal oral preparation is mainly prepared from radix bupleuri, fried radix paeoniae alba, roasted rhizoma atractylodis macrocephalae, saposhnicovia divaricata, roasted fructus aurantii immaturus, roasted pericarpium citri reticulatae, fructus mume, coptis chinensis and honey-fried licorice root, as well as auxiliaries. The detection method comprises the following steps: carrying out thin-layer chromatography identification on roasted rhizoma atractylodis macrocephalae, roasted pericarpium citri reticulatae, roasted fructus aurantii immaturus, fructus mume and honey-fried licorice root in the oral preparation and detecting the content of paeoniflorin and berberine hydrochloride. The detection method is accurate and has high sensitivity, good repeatability and stable detection result, the quality of the medicinal oral preparation for treating irritable bowel syndrome can be effectively detected, and the standard of each detection item can guarantee the curative effect of the medicine.

The invention discloses a thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules. The method comprises the steps that 1, sample powder is taken and dissolved through water, hydrochloric acid is added, heating is conducted for hydrolysis, filtration is conducted, filtrate is extracted through ethyl acetate or ethyl ether, extract liquor is evaporated to dryness, and residues are dissolved through methyl alcohol to serve as a test sample solution; 2, reference medicinal materials are taken, decocted through water and filtered, filtrate is concentrated, and then a reference medicinal material solution is prepared through the method for preparing a test sample; 3, the test sample solution and the reference medicinal material solution are sucked and dripped on a same silica gel GF254 thin-layer plate, and developing is conducted by taking a mixture of methylbenzene, one or more of ethyl formate or ethyl acetate or butyl acetate and formic acid as developing solvents according to the volume ratio of 2-8 to 2-8 to 0.1-1; 4, the thin-layer plate is viewed under a 254 nm ultraviolet lamp, and principal spots with the same color are showed on the positions, corresponding to the chromatography of the reference medicinal materials, in the test sample chromatography. The method is rapid, comprehensive and high in specificity, and the requirements of carbonized herbs and the carbonizing drug characteristic of the formula granules thereof can be embodied.

The invention discloses a manufacturing method of an individual hip joint percutaneous puncture guide plate. The manufacturing method comprises the following steps that 1, a three-dimensional geometric model is built, wherein thin-layer chromatographic scanning data of the hip joint of a patient is obtained, and three-dimensional models of skeleton, soft tissue, skin and important blood vessels are built and exported and stored in a stl format; 2, a puncture channel is determined, wherein in modeling software, a puncture target point and the needle entering direction and angle are determined, the puncturing needle form is extracted, a puncture channel is formed through fitting, and then the puncture channel is reversely prolonged to puncture out of skin to form a body surface marker to be exported and stored in an stl format; 3, the guide plate is designed, wherein in software, mesh optimization is carried out on the three-dimensional models of skeleton, soft tissue, skin and important blood vessels, a skin surface mesh is extracted, reverse offset thickening is performed to form a guide plate panel, a bone mark is marked, and the guide plate panel is in fit with the reverse puncture channel to obtain a guide plate model; 4, a guide plate entity is obtained through 3D printing. The manufacturing method has the advantages of being individual, accurate, rapid, low in cost and efficient.

The invention relates to a flavonoid glycoside compound in flos elaeagni angustifoliae, and a preparation method thereof. The method comprises the following steps: taking flos elaeagni angustifoliae medicines as raw materials, extracting by solvent, extracting by solvent, carrying out separation through two or three ways including positive phase and reverse phase silica gel column chromatography,or glucan gel LH-20 column chromatography and a semi-preparation high-performance liquid chromatography, adopting thin-layer chromatography and the high-performance liquid chromatography to detect andanalyze to obtain two new flavonoid glycoside compounds, wherein the first compound is flos elaeagni angustifoliae glycoside A, and the second compound is flos elaeagni angustifoliae glycoside B. Through the measurement of in vitro inhibition of COX (cyclo-oxygense) enzyme activity, an experiment result indicates that the flavonoid glycoside compound separated from the flos elaeagni angustifoliaecan inhibits the COX enzyme activity in different degrees, and the flavonoid glycoside compound in flos elaeagni angustifoliae can be used for preparing anti-inflammatory medicines.

The invention discloses a preparation method of a steroid intermediate. The preparation method comprises the following steps: adding a first solvent, a first acid solution, water and 17-alpha-hydroxypregnenolone steroid-4,9-diene-3,20-diketone acetate into a first reaction tank, and uniformly stirring; adding a bromination agent in four times at 2-4 DEG C, maintaining the temperature for reactingfor 2h, and carrying out thin-layer chromatography analysis until the reaction of the raw materials is finished; and regulating the pH value to be more than 7, regulating the pH value back to 6, carrying out reduced pressure concentration until the first solvent is completely concentrated, adding water, cooling to 5 DEG C, and carrying out centrifugation, cleaning, spin-drying and drying, so as toobtain the steroid intermediate, namely cortisone. According to the preparation method, an oxidant and a catalyst which contain heavy metal ions are not used, the used solvent can be recycled and reused, wastewater does not contain heavy metal ions in the production process, the method belongs to an environment-friendly process and has very good process prospects, and the yield and purity of theproduct are high.

A Chinese medicine Gongyanping for treating gynecological inflammations is prepared from 5 Chinese-medicinal materials including shing bugleweed herb, zanthoxylum nitidum, Chinese angelica root, hispid fig root, etc. Its quality control method features that the efficient liquid-phase chromatographic analysis method or UV spectrophotometric method is used for measuring the content of tannin or gallic acid, and the thin-layer method is used to measure the contents of the one or more active components contained in its raw materials.

The present invention is the new quality control method for Rehmanniae ointment of six ingredients. The medicine Rehmanniae ointment of six ingredients is prepared with six kinds of Chinese medicinal materials, including prepared rhizome of rehmannia, dogwood, Chinese yam, tree peony bark, tuckahoe and oriental water plantain. The quality control method includes the high efficiency liquid phase chromatographic process to measure the content of paeoniflorin and the thin-layer chromatographic process to identify dogwood, and the method is accurate and advanced.

The invention discloses a quality detection method of a "Zhenjiang" plaster. The "Zhenjiang" plaster is composed of a plaster content, fine powder, borneol, menthol, and turpentine; wherein the plaster content is composed of garter snake, notopterygium root, saposhnicovia divaricata, mustard, angelica pubescens, Chinese angelica, datura metel L, carbonized hair, nux vomica, ephedra, croton, dahurian angelica root, safflower, rhizoma sparganic, peach kernel, and dung beetle; and the fine powder is composed of raw monkshood, raw radix aconiti agrestis, rhizoma arisaematis, cassia bark, woodlouse and centipede. The detection method comprises steps of identifying radix aconiti agrestis, cassia bark, woodlouse, and ceptipede with a microscopic and identifying borneol and menthol through thin-layer chromatography. The quality detection method is simple and precise, the quality control standard is improved, an identification method of radix aconiti agrestis, cassia bark, woodlouse, and ceptipede and a thin-layer chromatography method of borneol and menthol are added, and the high quality control level of the "Zhenjiang" plaster is guaranteed.

The invention relates to a quality control method of chlorpheniramine maleate of Keteling troche, which is characterized in that the method includes a chlorpheniramine maleate thin layer chromatographic method examination of the Keteling troche pressing grain, a content measurement of the chlorpheniramine maleate with high-efficiency liquid phase chromatography, a chlorpheniramine maleate thin layer chromatographic method examination of Keteling troche finished product and a content measurement of the chlorpheniramine maleate with high-efficiency liquid phase chromatography. The quality control method of the Keteling troche provided by the invention adopts the chromatography and the color developing conditions to lead the chlorpheniramine maleate to effectively separate from other ingredients, the test result has good repeatability; the content of the chlorpheniramine maleate can be precisely measured in the Keteling troche, and the quality of the Keteling troche finished product is effectively controlled.

The invention discloses a quality detection method of a Houttuynia herb injection. The method comprises content measurement, identification and/or fingerprint detection processes. The method is a gas chromatography, comprising the following steps of: taking a 4-terpineol reference substance, an alpha-terpinol reference substance, a bronyl acetate reference substance and methylnnonylketone, respectively adding n-hexane to prepare a reference substance solution; adding the n-hexane in the Houttuynia herb injection, adding anhydrous sulfate to prepare a test sample solution; respectively absorbing the reference substance solution and the test sample solution; injecting in the gas chromatography to test; and detecting according to the method of content measurement items, and recording a chromatogram, wherein the similarity of the test sample fingerprint and the reference fingerprint is not less than 0.85. The invention further provides a detection method of polysorbate 80 limit test in the Houttuynia herb injection, an identification method of thin-layer chromatography and a detection method of essential oil content in Houttuynia herb medical materials or injections. The quality detection method provided by the invention has good specificity, good linear relation, good instrument precision and good repeatability.

The invention discloses a method of synthesizing aryl substituted azaspirocyclohexadienone by aromatizing a visible light induced (N)-benzylacrylamide compound and diazonium salt of fluoboric acid toform a ring. The synthesized target compound has the structure as shown in the formula as described in the specification. A preparation method comprises the following steps: carrying out a reaction bytaking the (N)-benzylacrylamide compound and the diazonium salt as a reaction primer for 18 h, Ru(bpy)3Cl2 as a catalyst, K2CO3 as alkali and DMF (N,N-dimethylformamide) as a solvent in a nitrogen environment, wherein the reaction temperature is room temperature; and carrying out column chromatography or thin-layer chromatographic separation and purification on the product through washing, concentration and the like to obtain the target product azaspirocyclohexadienone. The synthesizing reaction is simple to operate, the adopted free radical source is low in price and easy to obtain, the reaction system is mild and environmentally friendly, and the method is high in selectivity, simple in condition and high in yield, and has a huge popularization and application value.