277 results about "Thin layer chromatographic" patented technology

The invention discloses a tea oil adulteration detection method based on thin-layer chromatography and image analysis, and belongs to the technical field of tea oil adulteration detection.The tea oil adulteration detection method comprises the steps that firstly, tea oil with different adulteration concentrations is analyzed based on thin-layer chromatography, oil sample images are obtained, and therefore an image recognition database is established; the adulterated tea oil lipid and lipid adjunct with different concentrations have different color development positions under the action of the composite organic solvent, so that the adulterated tea oil machine identification model is constructed by taking different color development positions as characteristic indexes. And identifying a thin-layer chromatogram of a to-be-detected oil sample by adopting the tea oil machine identification model, and judging the adulteration condition of the tea oil according to an identification result. According to the method, the thin-layer chromatography analysis and the machine algorithm are combined, and the thin-layer chromatogram of the sample to be detected is identified and distinguished by utilizing the machine algorithm, so that the tea oil adulteration result and adulteration concentration can be obtained, and the method is simple, convenient and rapid to operate and easy to popularize and promote.

The present invention relates to a digital thin-layer chromatography detection and analysis model, a method for establishing the same, and an application thereof, belonging to the technical field of traditional Chinese medicine quality control. The digital thin-layer chromatography detection and analysis model is established based on the relative migration distance value (RPf) theory, and can be used for digital thin-layer chromatography analysis without physical reference. Under different experimental conditions and for different experimental varieties, the relative standard deviation of the RPf value is the smallest, showing better performance than RRf, and demonstrating the application advantages of the characteristic spot migration distance method in the digital standard of traditional Chinese medicine thin-layer chromatography.

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention discloses a compound donkey-hide gelatin pulp thin-layer chromatography fingerprint construction and combination evaluation analysis method, and belongs to the technical field of traditional Chinese medicine quality control. The compound colla corii asini pulp thin-layer chromatography is a thin-layer chromatography fingerprint spectrum of the compound colla corii asini pulp or an intermediate in a process preparation process; the construction of the thin-layer chromatography fingerprint spectrum is based on the color of a thin-layer chromatography sample strip image and the gray scale of spots, and a pixel-intensity matrix and a ratio shift value-gray scale matrix are formed to comprehensively reflect the thin-layer chromatography characteristics of the sample; according to the combined evaluation method of the thin-layer chromatography fingerprint spectrum, a method of combining machine learning with information entropy and entropy is adopted, ginsenoside Rg1, codonopsis pilosula alkynol and other components are screened and identified as key components influencing the compound donkey-hide gelatin pulp and transmission, and the method has the characteristics of accuracy and high efficiency, is wide in application prospect and can provide a thought for establishment of quality standards of traditional Chinese medicine compounds.

The invention discloses a thin-layer chromatography identification method for cinnamon and ginger in vitality-preserving granules, and belongs to the technical field of pharmaceutical analysis. Aiming at the problems of step-by-step detection, serious cinnamic acid tailing and dependence on toxic solvents in the existing method, the method comprises the following steps: dissolving vitality-preserving particles in water, carrying out ultrasonic treatment, extracting filtrate by using absolute ether, merging extract liquor, evaporating to dryness, and dissolving by adding ethyl acetate to obtain a test solution; preparing a cinnamon reference medicinal material solution and a cinnamic acid and 6-gingerol reference substance solution; a silica gel GF254 thin-layer plate is adopted, petroleum ether-normal hexane-ethyl formate-formic acid is used as a developing solvent for developing, and inspection is conducted under the ultraviolet light of 254 nm and the ultraviolet light of 365 nm respectively. Cinnamon and ginger can be synchronously identified, spots are clear and free of trailing, no toxic solvent exists, durability is high, cost is low, and an accurate and reliable basis can be provided for quality control of the vitality-preserving granules.

The present invention provides a thin-layer identification method for pinecone formula granules, comprising: A) taking a test sample, and after pretreatment, obtaining a test solution; taking a pinosylvin monomethyl ether reference substance, and after pretreatment, obtaining a reference solution; B) taking a reference medicinal material of pinecone, and after pretreatment, obtaining a reference medicinal material solution; C) performing thin-layer chromatography detection on the test solution, the reference solution and the reference medicinal material solution, wherein the thin-layer plate is a silica gel G thin-layer plate; the developing agent is toluene-ethyl acetate-glacial acetic acid; D) examining, in the test chromatogram, if spots of the same color appear at the positions corresponding to the reference medicinal material and the reference substance, it is pinecone formula granules. The thin-layer identification chromatographic method for pinecone formula granules established by the present invention uses, as the identification points, the spots of the same color that appear at the positions corresponding to the reference medicinal material and the reference substance by observing the thin-layer chromatogram, so as to quickly and effectively identify pinecone formula granules.

The application discloses a kind of gardenia and its processed product identification method, comprising: step one, respectively take equal amount of gardenia sample powder, gardenia processed product sample powder;Step two, the above-mentioned powder is respectively soaked with ethanol solution, then ultrasonic extraction is carried out, filtration, filter liquor is evaporated to dryness, obtain gardenia sample crude extract, gardenia processed product sample crude extract;Step three, the above-mentioned crude extract is respectively dissolved with water, then sequentially extracted with petroleum ether, ethyl acetate twice, and the two extraction liquids are combined and evaporated to dryness, obtain extract, the extract is dissolved with methanol, obtain gardenia sample test solution, gardenia processed product sample test solution;Step four, the above-mentioned test solution is respectively spotted on the same silica gel thin layer plate, with developing agent development, dry, with color developing agent color development, and under ultraviolet lamp, view thin layer chromatogram, according to the number, position, color of fluorescence spot on thin layer chromatogram, identify sample variety.

This invention discloses a quality control method for an anti-snoring oral liquid and its application, belonging to the field of traditional Chinese medicine quality testing technology. The method includes thin-layer chromatography identification of Gleditsia sinensis thorns, Scrophularia ningpoensis, and Glycyrrhiza uralensis, as well as high-performance liquid chromatography (HPLC) determination of hesperidin content. By optimizing pretreatment, developing solvent, and colorimetric conditions, this invention achieves efficient and specific identification and quantification of multiple components in the compound preparation. The method is highly specific, produces clear spots, has good separation, and is tolerant to a wide temperature and humidity range (2-40℃, 20%-75%RH), requiring no strict environmental control, significantly improving detection efficiency and providing a reliable basis for establishing quality standards for this preparation.

The invention relates to a method for detecting a clearing agent main agent in gasoline, and the method comprises the following steps: carrying out thin-layer chromatography detection on a to-be-detected sample, and determining the composition of the clearing agent main agent in the concentrated to-be-detected sample according to color spot distribution on an obtained thin-layer chromatography plate, wherein the clearing agent main agent comprises one or more of a polyether amine clearing agent, a polyisobutene amine clearing agent and a Mannich base clearing agent; the thin-layer chromatography detection sequentially comprises developing and developing treatment; a developing agent for developing comprises one or more of a developing agent I, a developing agent II and a developing agent III; the developing solvent I is methanol, and the developing solvent II and the developing solvent III comprise one or more of alcohol with the carbon atom number of 1-6, aliphatic hydrocarbon with the carbon atom number of 5-12 and cycloalkane with the carbon atom number of 5-12. According to the method provided by the invention, whether the clearing agent main agent is added into the sample to be detected or not can be rapidly determined, and the type of the clearing agent main agent can be rapidly and accurately detected.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

The present invention discloses a thin layer chromatography identification method for simultaneously identifying multiple components in a refined flat peach pill preparation and its application, which belongs to the technical field of quality control of traditional Chinese medicine. The thin layer chromatography identification method of the present invention is divided into two parts. The first part adopts methanol ultrasonic extraction and is developed by toluene-ethyl acetate-formic acid-methanol system, which can simultaneously identify the alpinia oxyphylla, wolfberry and fenxinmu in the refined flat peach pill; the second part is based on the methanol ultrasonic extraction, and hydrochloric acid is added to heat and reflux extraction, shaken and evaporated, and then ethanol is redissolved and developed by petroleum ether-ethyl acetate system, which can identify the ophiopogon in the refined flat peach pill. That is, only two sample preparation methods are required, and one thin layer plate can simply, quickly and effectively identify the four medicinal materials in the refined flat peach pill, and the color spots are clear, without tailing, without cross-linking and good specificity.

The invention relates to the technical field of thin-layer chromatography detection, and discloses a thin-layer chromatography separation method for amino polyether and a matrix in a fluorine-18 labeled radiopharmaceutical, and the adopted developing solvent is acetonitrile and ammonia water, and the volume ratio of the acetonitrile to the ammonia water is 90: 10-85: 15. According to the method, amino polyether and matrix components are separated by adopting a developing solvent with a specific volume ratio, and particularly, sodium ascorbate in the amino polyether and the matrix is separated.

The invention discloses a longan leaf granule which is prepared by the following steps: adding water into longan leaves, heating, extracting, filtering, concentrating, drying, crushing to obtain dry paste powder, adding a diluent into the dry paste powder, uniformly mixing, adding a wetting agent, granulating, and drying to obtain the longan leaf granule. The forming rate, the dissolution rate, the moisture absorption rate, the fluidity and the like of the granules all meet the requirements, and the granules are good in stability, suitable for industrial batch production, capable of treating diabetes, capable of meeting the requirements of doctors for treatment based on syndrome differentiation and increase and decrease along with symptoms and convenient to carry and take by patients. Thin-layer chromatography is adopted for qualitative identification of the longan leaf granules, high performance liquid chromatography is adopted for content determination of quercetin, quercitrin and kaempferol in the longan leaf granules, operation is easy, convenient and accurate, repeatability is good, and a scientific basis is provided for quality evaluation and control of the longan leaf granules.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to stasis-removing and pain-relieving pills and a quality detection method thereof. The stasis-removing and pain-relieving pill is prepared by taking radix paeoniae alba, rhizoma corydalis, radix paeoniae rubra, angelica sinensis, ligusticum wallichii, rhizoma cyperi, rhizoma zingiberis, fennel and astragalus membranaceus as raw materials and taking 10% starch slurry as an adhesive. The quality detection method of the stasis-removing and pain-relieving pill comprises general inspection, microscopic identification and thin-layer chromatography identification. And high performance liquid chromatography content determination. According to the present invention, by researching the preparation process and the quality standard of the stasis removing and pain relieving pill, the new thought and the new method are provided for the development of the traditional Chinese medicine preparation, the excellent choice is hopefully provided for the common gynecologic symptoms such as irregular menstruation, dysmenorrhea, postpartum blood stasis abdominal pain and the like, and the pain caused by the illness is relieved.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly discloses a thin-layer chromatography method for identifying radix angelicae lateralis preparata components in rhizoma coptidis paste and application of the thin-layer chromatography method. The thin-layer chromatography method provided by the invention comprises the following steps: preparing a test solution: adding methanol water into coptis chinensis paste, heating and melting, and then refluxing and stirring; storing at 0 + / -5 DEG C; then, dichloromethane-n-butyl alcohol with the volume ratio of (2.5-3.5): 1 is adopted as an eluent, silica gel column filtration is performed to obtain a filtrate, and the filtrate is evaporated to dryness and dissolved to obtain a test solution; preparing a reference solution; and detection: respectively dripping the solutions on the same silica gel G thin-layer plate, putting the silica gel G thin-layer plate into a developing cylinder to be developed by taking ethyl acetate-isopropanol with the volume ratio of (3-5): 1 as a developing solvent, and inspecting under a light source of 360-370nm. The thin-layer chromatography method provided by the invention can be used for rapidly and accurately identifying the radix angelicae lateralis preparata component in the rhizoma coptidis paste, and a theoretical basis is provided for quality control of the rhizoma coptidis paste.

Bottom-Interface Thin Layer Chromatography Chamber

The present invention discloses a preparation method of thiazolidine-4-thione derivatives, which comprises the following steps: (1) mixing an isothiocyanate or its derivative, a sulfur ylide, an additive and an organic solvent, and reacting at 20-90 °C for 12-48 h under an air atmosphere; (2) diluting the material obtained in step (1) with ethyl acetate and washing with water, and separating to obtain an organic phase; (3) subjecting the above organic phase to drying, filtration, concentration and column chromatography or thin-layer chromatography in sequence to obtain thiazolidine-4-thione derivatives. The present invention can construct tetra-substituted thiazolidine-4-thione derivatives, can efficiently synthesize thiazolidine-4-thione derivatives containing trifluoromethyl, the raw materials used are easily available, the yield is high, the reaction conditions are mild, the reaction time is short, the substrate range is wide, the reaction specificity is strong, and the post-treatment is simple.

The invention discloses a method for detecting glyceride and fatty acid composition ratio in mixed fatty acid glyceride by thin-layer chromatography-gas chromatography coupling, and the method mainly comprises the following steps: 1) internal standard solution is prepared; 2) mixed fatty acid glyceride need testing solution is prepared; 3) thin-layer chromatography is used to separate mixed fatty acid glyceride; 4) fatty acid methyl ester is prepared; 5) gas chromatography is used to detect, and the fatty acid composition of monoglyceride, diglyceride and triglyceride and the ratio of each fatty acid are determined respectively; and 6) the sum of the fatty acids of each monoglyceride, diglyceride and triglyceride obtained after data analysis in step 5) is divided by the fatty acid sum of monoglyceride, diglyceride and triglyceride, and the composition ratio of each monoglyceride, diglyceride and triglyceride in mixed fatty acid glyceride is obtained. The method of the present invention is simple and efficient to operate, and is quantitatively accurate, and methyl esterification reaction conditions are mild, equipment is simple, and no special equipment is required.

Disclosed is a thin-layer chromatography identification method for Rhizoma dioscoreae hypoglaucae formula granules. The method comprises: taking a sample to be subjected to detection, and dissolving s

The invention discloses a preparation method of a dialkynyl silane compound, which belongs to the technical field of organic synthesis and comprises the following steps: sequentially adding a reducing agent, an organic solvent, alkynyl bromide and chlorosilane into a reaction container, and reacting at 20-60 DEG C for 6-36 hours to obtain a mixture A; wherein R1 is allyl, alkyl or aryl; wherein R2 is allyl, alkyl or aryl; wherein R3 is an alkenyl group, an alkyl group, an alkoxy group or a silicon group; wherein R is halogen, alkyl, aryl, alkoxy, ester group, cyano or carbonyl; diluting the mixture A with ethyl acetate, adding silica gel powder, and performing vacuum concentration to remove the reaction solvent to obtain a mixture B; and separating the mixture B through thin-layer chromatography or column chromatography to obtain the target product dialkynyl silane compound. According to the method, dialkynyl silane substituted by different alkynyl groups can be obtained at the same time, the chemical selectivity is good, and dialkynyl silane which is not easy to obtain by other methods can be synthesized.

This invention belongs to the field of traditional Chinese medicine detection technology, specifically relating to a multi-component thin-layer chromatography (TLC) identification method for pediatric runtong syrup. This invention establishes specific TLC identification processes for roasted radish seed, immature bitter orange, hemp seed, and scrophularia root, optimizing the sample pretreatment, developing solvent ratio, spotting volume, and colorimetric inspection conditions for each component. After specificity and robustness studies, the method demonstrates good stability under varying temperature, humidity, TLC plate type, and spotting volume ranges, exhibiting no negative interference and clear spots, enabling accurate multi-component identification. This invention is the first to establish a TLC identification method for these four medicinal materials in pediatric runtong syrup, providing a scientific and reliable technical means for the quality control of this compound preparation and filling a gap in existing technology.

The present invention provides a thin-layer chromatography (TLC) identification method for Amomum villosum formula granules, comprising the steps of preparing a test solution, preparing a control medicinal material solution, and performing TLC identification. The test solution and the control medicinal material solution are prepared using a simple and rapid preparation method, and are spotted on the same TLC plate. After development, the TLC plate is inspected under ultraviolet light to obtain a multi-information TLC of the Amomum villosum formula granules. This method uses the control medicinal material as a reference and flavonoids as the main identification component. It has high specificity and can distinguish Amomum villosum of different origins (Amomum villosum and Amomum villosum var. amomi) and identify mixed and counterfeit Amomum villosum (Amomum villosum var. longifolia).

The invention discloses a flavonoid enantiomer compound extracted from Epimedium sagittatum and its preparation method and application. The preparation method comprises the following steps: crushing Epimedium sagittatum to prepare a paste, suspending the paste with water, extracting the paste with petroleum ether, dichloromethane, ethyl acetate and n-butanol respectively to obtain extracts of each part, performing gradient elution separation by silica gel column chromatography, combining similar parts according to the color development results of thin layer chromatography (TLC) to obtain multiple polar segments; chromatographing the target polar segment and performing gradient elution to obtain multiple components, and then combining similar components by thin layer chromatography (TLC) spot plate absorption to obtain multiple sub-components; eluting and purifying the target sub-components, collecting fractions with different retention times, and drying to obtain four compounds respectively. The four compounds have a significant inhibitory effect on the cell viability of human breast cancer MCF-7 cells and have anti-breast cancer activity. The four compounds can be used in the preparation of drugs for treating breast cancer, with huge social and economic benefits.

The invention relates to the technical field of cistanche deserticola detection, in particular to a method for simultaneously and rapidly detecting four phenylethanoid glycosides in cistanche deserticola, which comprises the following steps: preparing a plurality of parts of mixed reference substance solutions containing four phenylethanoid glycosides with known concentrations; preparing a to-be-detected cistanche sample into a test solution; spotting the mixed reference substance solution and the test solution on the same thin-layer plate, carrying out thin-layer chromatography development by adopting a developing solvent, and volatilizing the solvent; placing the thin-layer plate after the solvent is volatilized under ultraviolet light to collect spot images, and processing the images by adopting image analysis software to obtain integral gray values of all phenylethanoid glycoside spots; respectively drawing a standard curve by taking the known concentrations of the four phenylethanoid glycosides in each mixed reference substance solution as horizontal coordinates and the corresponding integral gray values as vertical coordinates, establishing a linear regression equation, and calculating the contents of the four phenylethanoid glycosides in the cistanche deserticola sample to be detected; the invention provides a rapid and accurate method for determining the content of four phenylethanoid glycosides in cistanche.

The present invention provides a thin-layer identification method for the formula granules of Lycopodiastrum casuarinoides and Lycopodium clavatum, comprising: A) taking a sample to be tested, and obtaining a test solution after pretreatment; B) taking the reference medicinal material of Lycopodiastrum casuarinoides, and obtaining a reference medicinal material solution after pretreatment; C) performing thin-layer chromatography detection on the test solution and the reference medicinal material solution, and the thin-layer plate is a silica gel G thin-layer plate; the developing agent is cyclohexane-ethyl acetate; D) visualizing under an ultraviolet lamp. In the chromatogram of the test sample, if there is a blue fluorescent spot at an Rf value of 0.31 to 0.464, it is the formula granules of Lycopodiastrum casuarinoides; if there is no blue fluorescent spot at an Rf value of 0.31 to 0.464, it is the formula granules of Lycopodium clavatum. The thin-layer identification chromatographic method for the formula granules of Lycopodiastrum casuarinoides and Lycopodium clavatum established by the present invention uses the presence or absence of fluorescent spots within a specified retention factor value range in the thin-layer chromatogram as the identification point to quickly and effectively identify the formula granules of Lycopodiastrum casuarinoides and Lycopodium clavatum.

A method for thin-layer chromatography detection of radix pseudostellariae in a ginseng-radix puerariae kidney-tonifying preparation. Raw materials of the ginseng-radix puerariae kidney-tonifying preparation comprise radix pseudostellariae, radix puerariae, and herba epimedii. The method for thin-layer chromatography detection of radix pseudostellariae in the ginseng-radix puerariae kidney-tonifying preparation comprises the following steps: preparing both a test sample solution and a radix pseudostellariae contrast medicinal material solution; spotting the test sample solution and the radix pseudostellariae contrast medicinal material solution onto a same thin-layer chromatography plate, developing with a developing solvent, and adding a color visualization agent for color visualization, then visualizing and detecting; wherein the developing solvent accounts for a volume fraction of 75% and is a mixed solvent composed of isopropanol and ethanol at a volume ratio of (1-9):1, and the color visualization agent is a mixed solution of aniline, diphenylamine, phosphoric acid and acetone.

The present invention provides a thin-layer identification chromatographic method for Celosiae Semen and stir-fried Celosiae Semen formula granules, including: spotting the test solution on a silica gel G thin-layer plate, developing under thin-layer chromatographic conditions, and inspecting; the thin-layer chromatographic conditions: using toluene-ethyl acetate-methanol as the developing agent. By comparing the thin-layer chromatogram of the stir-fried Celosiae Semen formula granules with that of the Celosiae Semen control medicinal material and the Celosiae Semen granules, the same position with a retention factor value at 0.55-0.67 should not show fluorescent spots, which is used as the identification point 1 of the stir-fried Celosiae Semen granules; by comparing the thin-layer chromatogram of the stir-fried Celosiae Semen formula granules with that of the Celosiae Semen control medicinal material and the Celosiae Semen granules, the same position with a retention factor value at 0.43-0.52 has one more fluorescent spot, which is used as the identification point 2 of the stir-fried Celosiae Semen granules. This method can quickly and effectively identify the stir-fried Celosiae Semen formula granules that have lost the form of the cut crude drug, with simple operation, high precision, high sensitivity, good stability, and has good application prospects.

The invention discloses a traditional Chinese medicine compound composition for treating insomnia and a quality detection method thereof. The traditional Chinese medicine compound composition is prepared from cassia twig, dried ginger, schisandra chinensis, raw Chinese yam, cortex moutan, inula flower, lophatherum gracile, ginger processed pinellia, raw coix seed, raw oyster shell and cortex albiziae. According to the pathogenesis characteristics of insomnia and the principle of syndrome differentiation and treatment of traditional Chinese medicine, the optimal raw material composition and dosage part ratio are screened out through a large number of experiments, the prepared traditional Chinese medicine compound can nourish the liver, calm the heart and regulate the spirit, can improve clinical symptoms such as short-term insomnia disorder, dizziness, palpitation, sweating and restlessness, and has a wide application prospect. The clinical curative effect of treating insomnia is obviously improved. Thin-layer chromatography qualitative identification and high performance liquid chromatography are adopted for quantitative detection, the content of active ingredients of the rhizoma zingiberis, the Chinese yam, the moutan bark and other medicinal materials in the formula is comprehensively and objectively detected and analyzed, and methodological detection shows that the high performance liquid chromatography provided by the invention is high in precision and good in repeatability and stability.

The invention discloses a tissue regeneration promoting jade red gel and a quality detection method thereof.The tissue regeneration promoting jade red gel is characterized in that on the basis of a single-factor test, the dosage of carbomer 940, medicine and triethanolamine is used as influence factors, and the optimal proportion of all components of the gel is determined through an orthogonal test method: the dosage of carbomer 940 is 1%, the medicine loading capacity is 11%, and the dosage of triethanolamine is 1%; the results of accelerated stability and long-term stability tests show that the preparation has good stability in the investigation period. According to the method, the optimal thin-layer chromatography development condition is screened out, the radix angelicae sinensis, the radix arnebiae seu lithospermi and the sangusis draconis in the tissue regeneration promoting jade red gel are subjected to qualitative identification research, and chromatographic conditions such as the optimal flowability composition and a gradient elution mode are screened out through a large number of experiments; according to the present invention, with the high performance liquid chromatography method, the contents of the effective components such as ferulic acid, liquiritin, imperatorin, ligustilide, isoimperatorin and the like can be simultaneously determined, and the methodological detection results show that the high performance liquid chromatography method has characteristics of high precision, high accuracy, good repeatability and good stability.

The invention discloses a heart-protecting granule identification and quality control method based on thin layer chromatography, and belongs to the technical field of medicine detection. The thin-layer chromatography identification method comprises the following steps: identifying astragalus membranaceus by taking astragaloside as a reference substance, identifying wine-treated rhubarb by taking chrysophanol and emodin as reference substances, identifying leech by taking a leech medicinal material as a reference substance, identifying liquorice by taking liquiritin and a liquorice medicinal material as reference substances, and identifying radix rehmanniae by taking catalpol as a reference substance. And identifying salvia miltiorrhiza by taking salvianolic acid B and salvia miltiorrhiza medicinal materials as reference substances, and identifying fructus aurantii by taking naringin and neohesperidin as reference substances. By establishing the thin-layer chromatography identification method of the heart-protecting granules, an exclusive developing solvent and a developing mode are adopted, accurate identification is achieved according to the characteristics of spot colors, fluorescence characteristics and the like of corresponding positions of a test substance chromatography, a reference substance and a reference medicinal material chromatography, and the method is easy and convenient to operate, low in detection cost and high in repeatability and specificity; and quality control can be effectively carried out in the production process of the heart-protecting granules.