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Process for extracting indoline amide alkaloid from purslane and detection methods for indoline amide alkaloid

A technology of indoline amides and purslane amides, which is applied in the field of pharmacy, can solve the problems that the detection method has not been reported yet, and achieve the effects of simple qualitative detection method, simple preparation process and low cost

Active Publication Date: 2013-03-20
广州壹木新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the extraction and separation method of the indoline amide alkaloid monomer compound and the detection method using HPLC / MS / MS have been reported, but the process and extract of the enriched preparation of the total indoline amide alkaloid extract The TLC and HPLC fingerprint chromatogram detection methods still have no report

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  • Process for extracting indoline amide alkaloid from purslane and detection methods for indoline amide alkaloid
  • Process for extracting indoline amide alkaloid from purslane and detection methods for indoline amide alkaloid
  • Process for extracting indoline amide alkaloid from purslane and detection methods for indoline amide alkaloid

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Embodiment 1

[0034] Example 1 Extraction and detection of indoline amide alkaloids from the Chinese medicinal material purslane

[0035] 1. Experimental Materials and Instruments

[0036] Experimental materials: Portulaca oleracea medicinal material was purchased in Jinan Jianlian Pharmacy in May 2010 (batch number 20100502, produced by Jinan Jianlian Traditional Chinese Medicine Co., Ltd. Chinese Herbal Pieces Factory), and was identified as Portulaca oleracea L. by Professor Xiang Lan. The reserved sample (specimen number MCX-06-1-4) is kept in the Department of Pharmacognosy, School of Pharmacy, Shandong University. Column chromatography filler: macroporous adsorption resin (Cangzhou Baoen Chemical Co., Ltd., Hebei Province), polyamide resin (Luqiao Siqing Biochemical Material Factory, Taizhou City, Zhejiang Province), Sephadex LH-20 (Switzerland GE Healthcare Bio -sciences company); thin-layer chromatography: polyamide film (Zhejiang Luqiao Siqing Biochemical Material Factory), revers...

Embodiment 2

[0053] Example 2 Extraction and detection of indoline amide alkaloids from the Chinese herbal medicine purslane

[0054] 1. Experimental Materials and Instruments

[0055] With embodiment 1.

[0056] 2. Experimental steps

[0057] (1) Take the dried purslane, and use 6 times the amount (weight multiple) of 60% ethanol (volume percentage) to reflux extract for 3 times, each time for 1 hour; combine the extracts and concentrate to 0.5g crude drug / ml;

[0058] (2) The above extract was added to a polyamide column (30-60 mesh) of 1.5 times the volume, eluted with 6BV of water, 1.5BV of 10% ethanol, and then 4.5BV of 50% ethanol, and then Elute with 3BV of ammonia water (ammonia water mass concentration 25%), combine 50% ethanol eluate and ammonia water eluate, concentrate and dry under reduced pressure to obtain the eluate;

[0059] (3) The above-mentioned eluate was dissolved with an appropriate amount of ethanol / water, centrifuged (3000r / min, 10 minutes), passed through a 0.4...

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Abstract

The invention discloses a process for extracting indoline amide alkaloid from purslane. The process comprises the following steps: (1) taking the purslane and carrying out reflux extraction by ethanol; (2) adding an extracting solution onto macroporous resin, eluting by ethanol, decompressing and concentrating; (3) adding a concentrated solution onto a polyamide column, eluting by ammonia water, decompressing, concentrating and drying; and (4) dissolving ammonia water eluate by a solvent, centrifuging, filtering, adding a filtered solution onto sephadex LH-20, eluting by the ethanol, collecting a yellow stripe part, decompressing, concentrating and drying to obtain an indoline amide alkaloid extract, wherein the yield of the extract is 2 mg / g in medicinal materials and the extract mainly contains purslane amides A and B. The invention further discloses a thin-layer chromatography detection method and an HPLC (high performance liquid chromatography) detection method for the indoline amide alkaloid extract.

Description

technical field [0001] The invention relates to a process for enriching and extracting indoline amide alkaloids from purslane, a traditional Chinese medicinal material, and a detection method for the indoline amide alkaloids, belonging to the field of pharmacy. Background technique [0002] Purslane (Portulaca oleracea L.) is an annual succulent herb of the family Portulaca. It is rich in resources and widely distributed in temperate and tropical regions all over the world. Traditional medicinal and edible plants are used. There are many records in the herbal medical books of the past dynasties in my country. It has the functions of clearing away heat and detoxifying, cooling blood and stopping bleeding. Modern pharmacological studies have shown that purslane has antibacterial, skeletal muscle relaxation, uterine excitatory, anti-inflammatory and analgesic effects, promoting wound healing, anti-atherosclerosis, lowering blood fat, lowering blood sugar, anti-cancer, anti-oxi...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P39/06A61P25/00G01N30/90
Inventor 向兰焦泽沼
Owner 广州壹木新材料科技有限公司
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