2772 results about "Reflux extraction" patented technology

The present invention relates to extraction and purification of functional components in Chinese herbal medicine and aims at provindg extraction and purification process of chlorogenic acid in honeysuckle in relatively high yield and purity. The techonlogical scheme includes twice reflux extraction of honeysuckle cut into sections with dilute alcohol, concentrating the extractive, flocculating for initial purification and final purification through macroporous resin or ethyl acetate extraction. The said process is simple and practical and has less loss of chlorogenic acid and high product yield and purity.

The present invention relates to process of extracting chlorogenic acid from honeysuckle and belongs to the field of medicinal chemical technology. The process includes the steps of: reflux extraction of honeysuckle with water or ethanol, filtering to collect filtrate, recovering solvent, ethanol deposition to eliminating impurity, letting stand overnight, filtering, decompression recovering solvent, adding water and letting stand overnight, filtering, eluting the filtrate in macroporous resin column, concentrating eluent, collecting chlorogenic acid component, eluting on polyamide column with eluent, collecting chlorogenic acid component, concentration, rectification to obtain chlorogenic acid product with content over 95 %. The present invention is suitable for industrial application.

The invention relates to a bacterial preparation for degrading petroleum and restoring petroleum polluted soil ecology and a preparation method thereof. The bacterial preparation for degrading the petroleum and restoring the petroleum polluted soil ecology is a composite colony consisting of bacillus megaterium, pseudomonas fluorescens, streptococcus faecalis and candida tropicalis through optimized combination, wherein the matching ratio of the components is 1-2:1:1:0.5-1. The bacterial preparation has the advantage that the bacterial preparation can quickly form a micro-ecological advantage colony after the bacterial preparation is applied to the soil. If 2 to 5 percent of the bacterial preparation is added relative to the weight of surface soil (a cultivation layer of about 15 to 20 centimeters), the oil removal rate in three months is more than or equal to 75 percent (dichloromethane reflux extraction and gravimetric method), and the bacterial preparation has high effective colony number and strong adaptability and has remarkable effect of restoring the petroleum polluted soil ecological environment.

The invention provides a method for extracting new chamaejasmine B from stellera chamaejasme. L, which comprises the process steps of: reflux-extraction after pulverization of stellera chamaejasme. L, alkali extraction and acid precipitation, separation with macroporous resins and crystallization. The method has the advantages of simple operation, low carbon, economy and high purity of product.

The invention provides health wine raw materials, which comprise the following components in part by weight: 8 to 12 parts of Siberian solomonseal rhizome, 6 to 12 parts of fragrant solomonseal rhizome, 4 to 10 parts of raspberry; 2 to 8 parts of medlar, 2 to 8 parts of Chinese date, and 4 to 12 parts of dried longan pulp. The invention also provides preparation methods of two kinds of health wine, which comprises the following steps of: a) soaking the health wine raw materials in distilled spirit, filtering, and adding white granulated sugar into the obtained filtrate, dissolving and uniformly mixing to obtain the health wine; b) performing heat reflux extraction on the health wine raw materials by using solution of ethanol at proper concentration, filtering, recovering a solvent from the obtained filtrate to obtain extract, adding the distilled spirit into the extract to blend, then adding the white granulated sugar, dissolving, uniformly mixing and filtering to obtain the health wine. Compared with the prior art, the health wine prepared from the components by the two different preparation methods has the advantages of resisting fatigue, increasing immunity and building body, mainly because the Chinese date and the dried longan pulp, and the Siberian solomonseal rhizome, fragrant solomonseal rhizome, raspberry and medlar can supplement each other.

The invention discloses an extraction method of filament eucommia rubber from eucommia leaves and peel; the method uses petrol ether as solvent to extract the eucommia rubber and has the steps: first, the eucommia leaves or the peel is made into small pieces and added with NaOH solution to dissolve cuticle; then cellulase is added to hydrolyze cell wall; the petroleum ether solvent at 60 DEG C to 90 DEG C of boiling range is added; then the obtained solution is extracted through recirculation at the temperature of 85 DEG C and filtrated when being hot, then cooled and frozen; the filament eucommia rubber is produced after filtration. Concentration of the NaOH solution is 0.5 percent to 1.5 percent and the dissolution time is 6 hours; pH value of the cellulase used for hydrolyzing the cell wall is 4 at temperature of 50 DEG C; enzymatic hydrolysis time is 1 hour to 2 hours and the time for reflux extraction is 2 hours; the frozen time is 30 minutes to 60 minutes at temperature of minus 20 DEG C to 0 DEG C. The method can effectively and completely extract the eucommia rubber to make original eucommia rubber and keep the original biological characteristics and physical properties and status and the original molecular structure and polymerization of the eucommia rubber; the method uses the cellulose to extract pretreated eucommia leaves raw materials, and gets the eucommia rubber of high quality when getting high yield.

The invention discloses a compound lightyellow sophora root-selinum japenious seed bacteriostatic lotion and a preparation method thereof. The lotion contains an extract of lightyellow sophora root, amur corktree bark and selinum japenious seed, chlorhexidine acetate, a surfactant, ethanol and water, wherein the surfactant is selected from lauryl dimethylamine oxide, Alcohol Ethoxylate (AEO-9), Lauric Acid Ethoxylate (LAE-9) and nonylphenol polyoxyethylene ether (NPE-10); the extract is prepared by performing reflux extraction on 10-25 percent of lightyellow sophora root, 35-45 percent of amur corktree bark and 35-45 percent of selinum japenious seed by taking 50-70 percent by volume of an ethanol aqueous solution as an extraction solvent for 1-3 times, every time for 1-1.5 hours, combining extracting solutions, filtering, recovering a filtrate under reduced pressure in vacuum, and concentrating with ethanol till the relative density is 1.08-1.12; and the using amount of the ethanol aqueous solution is 6-8 times of the total mass of the lightyellow sophora root, amur corktree bark and selinum japenious seed. The compound lotion consisting of Chinese native medicament and western medicament components has the effects of killing parasites to relieve itching, clearing heat, drying dampness and clearing away heat and toxic materials, can play a role in protecting private skin mucosa and preventing bacteria invasion, has low cost, and is suitable for young and middle-aged women.

The invention discloses the preparation of a compound polysaccharide humectant and application of the compound polysaccharide humectant to cigarettes. The preparation method comprises the following steps of: (1) crushing compound Chinese medicines such as Adenophora elata, dwarf lilyturf tuber, and platycodon grandiflorus, adding water, slightly boiling, performing reflux extraction, mixing extracting solution, and filtering; (2) adding protamex and high-temperature amylase into the extracting solution, and performing enzymolysis; (3) absorbing naturally cooled enzymolysis extracting solution by using a macroporous resin column, and collecting flow-through liquid; (4) concentrating the flow-through liquid in a rotary evaporator until the moisture is not evaporated to obtain the polysaccharide humectant; and (5) dissolving the polysaccharide humectant in water and adding into cut tobacco, wherein the humidification effect of cigarettes is remarkably improved, and the dry sensation of the cigarettes is reduced and the sweet degree of the cigarettes is strengthened when the cigarettes are smoked. The invention has the outstanding advantages that: the compound polysaccharide humectant can improve the aesthetic quality of the cigarettes, reduce the dry sensation of smoke, improve the sweet degree and comfort degree, and has remarkable effects of promoting the secretion of saliva or body fluid and humidifying; and the application of the technology cannot change the conventional cigarette processing technology, and the technology has actual application value.

The invention discloses a process method for synchronously producing pueraria starch and pueraria flavonoid. The process method is characterized by comprising the following steps of: adding water to clean a fresh pueraria raw material, crushing the fresh pueraria raw material after cleaning, and adding water to grind the raw material into thick liquid, wherein the ratio of liquid to the raw material is 1:10; screening powder to remove residues by adopting a filter method to obtain pueraria starch slurry and pueraria residues mainly comprising fibers respectively, and drying the pueraria residues for later use; performing centrifugal separation on the pueraria starch slurry to remove protein and separate waste water, and collecting the waste water for later use; performing centrifugal dewatering and drying on the starch thick slurry after removing the protein and the waste water through separation to obtain the pueraria starch; soaking the pueraria residues obtained by the step (2) in ethanol, and performing reflux extraction, and condensing the extracting solution to obtain pueraria crude total flavonoid; standing the waste water obtained by the step (3) for deposition, taking supernatant fluid, and performing column chromatography through macro-porous polymeric adsorbent to adsorb the flavonoid; eluting the flavonoid through a resin column by using ethanol, and concentrating the eluant to obtain the pueraria crude total flavonoid.

The invention provides a method for extracting refined high-purity tea saponin by taking camellia oleifera seed cake as raw material. The method comprises the following steps: smashing the camellia oleifera seed cake after squeezing and oil extraction; carrying out reflux extraction with 70-95% ethanol; adding chitosan (the volume ratio of the chitosan solution to extraction liquid is 9-13:100) to the extraction liquid for flocculation and purification; eluting and removing impurities from the purified clear liquid with a sodium hydroxide solution after the purified clear liquid is absorbed by AB-8 macropore adsorbent resin; eluting the tea saponin with 60-90% ethanol; decoloring the eluent containing the tea saponin with hydrogen peroxide (the volume ratio of the hydrogen peroxide to the eluent is 3-6:100); and steam drying the decolored solution to obtain milky powdery tea saponin. The invention has the advantages of simple process, no pollution and low production cost, the purity is higher than 95%, and the tea saponin has good color and can be widely applied to the fields of daily chemical industry, textile, medicine, food industry and the like.

The invention belongs to the technical field of poultry feed and especially relates to a Chinese herbal medicine feed additive for laying hen and a preparation method thereof. The preparation method of the Chinese herbal medicine feed additive for laying hen comprises the following steps of respectively carrying out reflux extraction of motherwort, prepared rehmannia root, cape jasmine fruit, malaytea scurfpea fruit and dried orange peel orderly by 60 to 90% of ethanol and drinking water, merging extract, mixing the merged extract and water extract solutions of radix astragali, barbary wolfberry fruit, root of pilose asiabell, Chinese angelica, cape jasmine fruit, rhizoma atractylodis macrocephalae, cortex eucommiae, haw, isatis root, raspberry, ligusticum chuanxiong hort and white peony root, concentrating the mixed solution, drying , carrying out processing to obtain dry powder, uniformly mixing the dry powder, crushed pine needle powder and crushed hot pepper powder, and carrying out packaging. The preparation method provided by the invention adopts the scientific and reasonable raw materials and has strong operationality in preparation. The Chinese herbal medicine feed additive for laying hen allows a trace dose, has high efficiency and good palatability, is safe and nontoxic, and improves laying performances of laying hen, egg quality and nutritive values, and economic benefits of a laying hen culture.

The invention relates to an extraction method of an active substrate, and in particular relates to a method for extracting an active substance from the sea cucumber processing waste. By taking the viscera of sea cucumber as a raw material, the method is used for preparing refined sea cucumber polysaccharide through the processes of pretreatment, enzymolysis, reflux extraction, microwave extraction, alcohol precipitation, decoloration, centrifugation, salt precipitation, dialysis, refining and the like; the supernate after alcohol precipitation is subjected to the processes of rotary evaporation, extraction degreasing, water saturation n-butyl alcohol extraction, rotary evaporation, macroporous adsorption resin chromatography and the like to obtain refined sea cucumber total saponins; and the aqueous solution after the water saturation n-butyl alcohol extraction is processed by the technologies of decoloration, sterilization and the like to obtain antioxidant active sea cucumber polypeptide. The method provided by the invention turns the waste of sea cucumber viscera into wealth so as to obtain three products including sea cucumber polysaccharide, sea cucumber total saponins and antioxidant active sea cucumber polypeptide.

The invention discloses a method for preparing fungi polysaccharide, namely a multi-stage dissolvent-cycle dynamic-thermal-reflux extraction condensation method. The method comprises the technological steps of raw material crushing, mixing with a solvent, extraction, condensation, deposition in alcohol, filtering and drying so as to obtain a finished product. The method for preparing fungi polysaccharide integrates extraction, condensation, solvent recovery and the like together, and is completely carried out in an enclosed system, without any bad occurrence of escaping, steaming, leakage and dripping, etc. The production practice shows that the method can shorten the extraction cycle by 50 percent to 85 percent so as to improve the production rate; the ration between raw material to solvent can be reduced to 1:3-8 (as the traditional method has a ration between raw material and solvent higher than 1:20), and the amount of an extractant used can also be saved by 25 percent to 80 percent; primary/secondary steam can be put into fullplay and cooling water is saved so that the effect of energy conservation is very significant. Compared with the traditional method, the method can save 30 percent to 70 percent of steam amount; and the medical material does not need to be crushed finely, which is favorable to the further operation. The method can be largely applied to the development and production of the fungi polysaccharide and other related polysaccharides or natural medical materials.

The invention discloses a method for preparing the ursolic acid with the paulownia leaves as raw materials, comprising the following steps that: 1. the dry paulowina leaf powders with a particle size ranging from 20 to 60 meshes are taken and 3 to 5 times amount of the ethanol with a concentration over 90 percent is added in the dry paulowina leaf powders with carrying out the reflux extraction; the reflux extraction is conducted for 1 to 2 hours each time and goes for altogether 3 times; the ethanol-extracts are combined and are filtered so as to remove the impurities; the filtrates are driven to rest for over 12 hours after the ethanol is recycled and the precipitates are collected after filtration; the precipitates are dried and then the brown grey or black brown crude products of the ursolic acids are obtained. 2. the crude products of the ursolic acids are dissolved using the ethanol with a concentration ranging from 50 percent to 70 percent and the macroporous adsorption resin is used with carrying out the dynamic absorption; the solution is subject to the absorption till the solution approaches the saturated state before the solution is washed using 2 to 3 times of the deionized water by resin concentration for 3 to 5 times and then the obtained solution is eluted using the ethanol solvent with a concentration ranging from 85 percent to 99 percent; the precipitates are decolored using the activated carbon before the precipitates are crystalized for 2 to 3 times using the double solvents of the ethanol so as to obtain the target products. The advantages of the invention lie in that the goal of the industrialized batch production of the ursolic acids is realized with the paulownia leaves having wide sources as raw materials, and the purity and stability of the products both exceed 95 percent and no discharge of waste gas, waste residue and organic solvents exists in the invention.

The invention discloses a liquid bandage and a preparation method. The liquid bandage comprises the following raw materials: polyvinyl acetal, dibutyl phthalate, methyl cellulose, glycerol, laurocapram, chitosan, 95% ethanol, securidaca inappendiculata, dragon blood, frankincense, myrrh and sanchi; the preparation method comprises the following steps: stirring and dissolving the polyvinyl acetal,the chitosan and the ethanol at a temperature of 5 DEG C, and adding the laurocapram, the glycerol, the dibutyl phthalate and the methyl cellulose to obtain film liquid; performing reflux extraction on the crushed securidaca inappendiculata, dragon blood, frankincense, myrrh and sanchi with ethanol at a temperature of 60 DEG C until the extracted liquid is colorless, filtering at a normal pressure and concentrating; and adding the concentrated solution of the securidaca inappendiculata, the dragon blood, the frankincense, the myrrh and the sanchi into the film liquid and mixing uniformly. Theliquid bandage is convenient to use and has high water resistance and air permeability, without sticking on clothes and cotton-padded mattress, causing anaphylaxis and drug resistance or being limited by the shape, size and location of the wound.

The invention relates to a method for preparing simplified high-purity bulleyaconitine A, which comprises the following steps of: immersing tuberous root of kusnezoff monkshood root in 1 to 2 percent alkaline solution; crushing the tuberous root; performing heat reflux extraction with gasoline or petroleum ether 6 and the like; loading the extract; eluting the tuberous root with solvent gasoline, ethyl acetate and triethylamine or diethylamine in a volume ratio of 90-95 to 1-10 to 1-5; concentrating the eluent under reduced pressure till the eluent is dry; then thermally dissolving the eluent with the solvent gasoline; standing the obtained solution for 20 to 24 hours at normal temperature; crystallizing, filtering and drying the solution to obtain the crude product of the bulleyaconitine A; fully dissolving the crude product in methanol on a water bath of 45 to 55 DEG C; filtering the obtained solution and preserving the heat for 30 minutes on the water bath of 45 to 55 DEG C; adding pure water or distilled water dropwise to assist in the crystallization; standing the solution for 20 to 24 hours at normal temperature to form a crystal; performing filtration to remove the liquid part; washing the crystal for three times; and pumping the crystal and drying the crystal to obtain the finished product. The process has the advantages of simplified production flow, low cost, no three-waste emission, simple operation, high production safety and high extraction rate of the bulleyaconitine A.

The invention relates to a preparation method of epimedium aglycone, which comprises the steps that dried epimedium is pulverized or cut into segments, 30-95% ethanol or 30-100% methanol is added for reflux extraction, the extract solution is decompressed and concentrated to obtain paste, 10-100 ml of acetate-sodium acetate buffer solution with the pH of 3.5-9.0 is added into each gram of paste, snailase is added for hydrolysis, the mass ratio of enzyme to paste is 0.05-5, the reaction temperature is 15 to 80 DEG C, the reaction time is 2 to 72h, the reaction solution is centrifuged, the precipitate is dissolved with an organic solvent, filtration is carried out to obtain filtrate, the solvent is steamed away to obtain crude epimedium aglycone, and pure epimedium aglycone is obtained after recrystallization. The invention has the characteristic that the epimedium extract is directly converted into high-purity aglycone in one step by utilizing the strong enzymic hydrolysis of the snailase so as to simplify the operation steps with strong purposiveness and high conversion rate, and has the advantages of no pollution and large-scale production. The invention overcomes the shortcomings of large destruction on the aglycone, serious pollution, poor purposiveness, low conversion rate, difficult separation and purification and the like of the prior art.

The invention relates to a method for extracting tea saponin by adopting a tea seed cake, comprising the following steps of: firstly smashing a dry tea seed cake after tea seed oil is extracted, and then placing into a reaction vessel; adding the petroleum ether with the mass concentration of 90-95 percent according to a proportion of the mass/volume ratio of 1:3, and stirring at constant temperature for reaction at the temperature of 55 DEG C to carry out derosination for 1 hours, and filtering after the reaction, wherein the mass of tea seed powder is expressed as grams and the volume of petroleum ether is expressed as ml;; drying filtered filter residues, and then placing into the reaction vessel; adding ethanol with the mass concentration of 80-95 percents according to the proportion of the mass ratio of 1:5-7, and stirring to carry out reflux extraction at 65-75 DEG C by two times for 1.5-3.5 hours, and filtering filter liquor when the filter liquor is hot; distilling the filter liquor, and concentrating to one tenth of original volume at temperature which is not higher than 60 DEG C; adding acetone equivalent to two times of the liquid volume of concentrated liquor to the concentrated liquor for extraction, wherein faint yellow sediments are generated during the extraction; and carrying out vacuum drying on filter residues to obtain faint yellow rough saponin at normal temperature or low temperature.

The invention discloses a technology for the steam explosion treatment of a yellow wood fruit and the comprehensive utilization of grease and flavone thereof, and the like. In the method, the yellow wood fruit is firstly arranged in a steam explosion reactor and is pumped with saturated steam for treatment for 3 to 10 minutes under the pressure condition of 1.4MPa to 2.0MPa; then the mixture is quickly discharged from the reactor for explosion; a blank obtained through the steam explosion is naturally dried or arranged in a drying box for drying; an organic solvent is added for reflux extraction; a rotary evaporator is used for decompressing and evaporation; the solvent is recycled, thus obtaining the oil of the yellow wood fruit. An ethanol liquid is added into the oil meal and then a flavone liquid is obtained through reflux extraction. The remainder after extracting the grease and the flavone are manufactured into active carbon through thermal treatment, thus realizing the comprehensive utilization of the yellow wood fruit.

The invention discloses a coreopsis tinctoria nutt extract and application of the coreopsis tinctoria nutt extract in preparation of medicines for treating hyperlipemia and anti-oxidation medicines. A method for preparing the coreopsis tinctoria nutt extract comprises the following steps: performing reflux extraction on a coreopsis tinctoria nutt medicinal material for 2-3 times by 12-25 times of 30-75% ethanol the amount of which is 12-25 times of the amount of the coreopsis tinctoria nutt medicinal material; mixing extract liquids, and concentrating to obtain extracting concentrate; diluting the extracting concentrate by water; refining by an AB-8 macroporous resin; sequentially eluting by water, 10% ethanol, 50-75% ethanol and 95% ethanol, and collecting the part eluted by 50-75% ethanol; concentrating and drying. The coreopsis tinctoria nutt extract comprises 10 flavones serving as main components, wherein the total content of marein and flavanomarein is more than 10 percent. Animal in-vivo experiments indicate that the coreopsis tinctoria nutt extract has a certain blood-fat regulating effect; and in-vitro anti-free radical scavenging experiments indicate that the coreopsis tinctoria nutt extract has a certain anti-oxidation effect, and flavanomarein, marein and the like are main anti-oxidation components.

The present invention is process of extracting and separating ursolic acid from plant. The extracting and separating process of ursolic acid includes methanol reflux extraction of coarse paulownia leaf powder, recovering methanol to small volume, settling, filtering to eliminate filtrate, washing the precipitate with acetone for many times to eliminate impurity, and re-crystallization for three times in anhydrous alcohol and methanol to obtain ursolic acid. Compared with other processes of extracting ursolic acid, the present invention has the advantages of cheap material, simple technological process, low cost and being suitable for industrial production.

The invention provides a preparation method for a selenium-rich peony seed essential oil, and relates to the technical field of edible oil. The preparation method comprises the following steps: (1) carrying out fine selection and air drying on seeds, (2) carrying out shelling and roasting-frying, 3) carrying out hydraulic pressing and filtration, (4) carrying out deacidification, (5) carrying out degumming, (6) carrying out drying and decolorization, (7) carrying out vacuum deordorization, and (8) adding an antioxidant to obtain the peony seed essential oil, wherein the standby solid impurity residue in the step (3) is broken up, and is added with acetic ether, then the resulting material is subjected to reflux extraction, and reduced pressure solvent recovery is performed to obtain the peony seed essential oil for cosmetics. The preparation method of the present invention has the following beneficial effects that: the two-step method is adopted to extract the peony seed oil, physical pressing is adopted in the first step, and the produced peony seed oil has characteristics of high oil yield, no solvent residue and edibility; the acetic ether extraction is adopted in the second step, such that the anti-oxidation component, the anti-inflammatory component, the sunscreen component and other components in the peony seed oil can be completely extracted to be used for cosmetic production so as to effectively improve oil yield of the peony seeds and completely utilize active components in the peony seeds.

The invention provides a method for extracting solanesol and chlorogenic acid from discard tobacco leaves. The method comprises the following steps: (1) subjecting the discard tobacco leaves to reflux extraction with an aqueous ethanol solution with a concentration of 90 to 97% at a temperature of 35 to 40 DEG C and then filtering an extract with a ceramic membrane; (2) subjecting the extract to reduced pressure condensation at a temperature of 38 to 42 DEG C so as to obtain a dried substance; (3) dissolving the dried substance with water, carrying out filtration with the ceramic membrane, subjecting a filtrate to reduced pressure condensation so as to obtain a concentrate and standing a filter residue for subsequent usage; (4) dissolving the filter residue with ethyl acetate, carrying out filtration with the ceramic membrane, carrying out chromatographic separation by using a silica gel column, carrying out elution with absolute ethyl alcohol and subjecting an eluate to condensation and then to crystallization so as to obtain solanesol; and (5) carrying out chromatographic separation on the concentrate obtained in step (3) by using a macro-porous resin, carrying out elution with an aqueous ethanol solution with a concentration of 55 to 65%, condensing an eluate, adjusting a pH value to 3 to 3.5 and carrying out crystallization so as to obtain chlorogenic acid. The method provided by the invention has a simple process, is convenient to operate, effectively improves the utilization rate of the discard tobacco leaves, reduces production cost for chlorogenic acid and solanesol, has high yield and can effectively guarantee large scale production.