38 results about "Pedunculoside" patented technology

This invention relates to ilexin, drug combination of ilexin and its extraction method, which is used to treat CHD, angina pectoris, stroke, cardiac infarction and other cerebral vascular diseases. It uses ethanol for extraction, adds water into the extract, centrifuges to get the sediments and supernatant. Sediments are added 50-95% ethanol for extraction and filtration, the liquid is concentrated, placed and filtrated, and the sediments are dried to get a part of total saponin, the saponin is crystallized by ethanol to get a part of ilexin. The supernatant is enriched and centrifuged for the sediments, which is washed with appropriate water and crystallized by ethanol after dried to get another part of ilxin. Two parts of ilxin are put together and recrystallized for several times to get the pure ilxin, which content can be more than 98.0%.

The invention discloses a method for simultaneously preparing high-purity syringin and pedunculoside. According to the method, the high-purity syringin and the high-purity pedunculoside are prepared by using holly barks as a raw material, adopting a solvent extraction method and a macromolecular resin adsorption method for extraction and separation and adopting re-crystallization, open ozone depleting substance (ODS) column chromatography or Se-phadex LH-20 column chromatography, liquid chromatography and the like. The purity of each of the syringin and the pedunculoside is over 90 percent, and the method is simple, feasible and suitable for industrialized production.

The invention discloses an ilicis routundae cortex medicinal composition for preventing and treating alcoholic liver damage, alcoholic fatty liver, non-alcoholic fatty liver and hyperlipidemia. The composition is extracted and refined from natural ilicis routundae cortex and is not chemically modified, and is characterized by comprising substances such as pedunculoside, syringin, rotundicacid and the like. The invention also discloses a preparation method of the ilicis routundae cortex composition and application to preparation of a medicament for preventing and treating the alcoholic liver damage, the alcoholic fatty liver and the non-alcoholic fatty liver and lowering blood fat.

The invention discloses a two-step simultaneous preparation method for pedunculoside and syringin and belongs to the technical field of natural product extraction and separation. The method comprises the following steps: adding 20-70% (volume fraction) of ethanol water into ovate leaf holly bark, performing reflux extraction, collecting an extracting solution, standing and filtering the extracting solution, and respectively collecting filtrate and residues; 2 drying the residues to obtain crude pedunculoside, and purifying the pedunculoside to obtain the pedunculoside; 3, performing column chromatography separation to the filtrate with macroreticular resin, taking an ethanol-water system as an eluting agent to conduct gradient elution, concentrating and drying elution components to obtain crude syringin, purifying the crude syringin to obtain the syringin. The method is simple and feasible, has a short separation time and high separating efficiency, the purity of the pedunculoside and the purity of syringin can both reach more than 98%, and the method is suitable for large-scale industrial production.

The invention discloses the comprehensive use of an ovateleaf holly bark medicinal material, which relates to method for extracting and separating from plants and use in field of medicines, in particular to a method for extracting syringin, pedunculoside, ovateleaf holly bark acid and ovateleaf holly bark total saponins from an ovateleaf holly bark medicinal material and use. In the invention, the syringing is prepared from water extract solution of the ovateleaf holly bark medicinal material, the pedunculoside and ovateleaf holly bark acid are prepared from medicine slag obtained after water extraction from the ovateleaf holly bark medicinal material, the purities of the syringin, pedunculoside and ovateleaf holly bark acid are all over 98 percent, and the ovateleaf holly bark total saponins are extracted from the medicine slag obtained after the water extraction from the ovateleaf holly bark medicinal material. The ovateleaf holly bark total saponins can be used for preparing medicines for preventing and treating hyperlipemia, medicines for relieving myocardial ischemia and angina and medicines for treating inflammation and pain. The invention has the advantages that: four active ingredients can be obtained from the ovateleaf holly bark medicinal material and resources can be fully utilized; and 2, the raw materials are readily available, the process is simple, the quality is controllable and batch production can be performed.

The invention discloses application of pedunculoside in preparing a medicine for treating coronary heart disease. An effective dose of pedunculoside and a pharmaceutically acceptable carrier are prepared into the medicine for treating coronary heart disease. The medicine can be pills, oral liquid, injection, instillation, freeze-dried powder, microcapsules, capsules or tablets. The medicine can be used for preventing and treating coronary heart disease and angina pectoris in clinic, and other diseases with other symptoms such as obstruction of qi in the chest, vertigo, short breath, palpitation, chest distress, or pain and the like.

The invention discloses a traditional Chinese medicine based narcotic drug used for a tooth extraction operation and a preparation method thereof. The traditional Chinese medicine based narcotic drug comprises the raw medicines as follows: flos rhododendri, rubia cordifolia, flos daturae, pentapetes phoeniceam, glaucescent fissistigma root, carbo of typhae pollen, membrana follicularis ovi, cutch, alangium chinense harms, illicium verum, stellaria media, honeysuckle, herb of pedunculoside and radix angelicae. The traditional Chinese medicine based narcotic drug provided by the invention is prepared based on traditional Chinese theory of traditional Chinese medicine, has the advantages of being free of toxic and side effect, good in effects on relieving pain and stopping bleeding, fast to come into effect and low in cost, does not need injection when in use; when in use, dental tweezers are operated to clamp a cotton ball to drop or apply the liquid medicine into a periodontal pocket of a tooth to be extracted; when the periodontium has no pain, the tooth extraction operation can be carried out; therefore, the feared state of mind of a patient, especially a child and the patient fearful of giving an injection or the patient fainting on acupuncture, can be quelled.

The present invention relates to an ilex pedunculosa glycoside for curing cardio-cerebral vascular system diseases of coronary heart disease, angina pectoris, apoplexy and cerebral infarction, medicine composite containing ilex pedunculosa glycoside and its extraction separation method. Said extraction separation method includes the following steps: using ethyl alcohol to extract ilex pedunculosa to obtain extract, adding water into said extract, making centrifugal separation, concentrating supernatant fluid, making centrifugalization, washing precipitate by using proper quantity of water, drying and using ethyl alcohol to make crystallization so as to obtain the ilex pedunculosa glycoside pure product whose content can be up to above 98.0%.

The invention discloses a preparation method of pedunculoside. The method sequentially comprises the following steps of: (a) crushing leaf of purpleflower holly, adding into an extraction kettle, and collecting pedunculoside extract by using supercritical CO2 extraction; (2) dissolving the extract with hot water, filtering, adding a macoporous resin column into filtrate to adsorb, eluting with water to remove impurities, eluting with 30-50 percent ethanol solution, concentrating eluate under reduced pressure, standing at low temperature and centrifuging to obtain precipitate; and (3) recrystallizing the precipitate with ethanol to obtain the pedunculoside. The preparation method is simple, suitable for industrial large-scale production, low in energy consumption and low in pollution and is an environment-friendly production process.

The invention relates to a preparation method of pedunculoside. The method includes the following steps of using the traditional Chinese medicinal material ovateleaf holly bark as the raw material, conducting heating reflux extraction through a 20%-70% ethyl alcohol aqueous solution, concentrating filtrate till no alcohol smell exists to obtain extract, dissolving and evenly mixing the extract in a 30%-70% ethyl acetate aqueous solution, standing overnight, conducting filtering to obtain filter residues, and drying the filter residues to obtain pedunculoside. Compared with the prior art, the method has the advantages of being simple in process, short in period, low in cost, high in product productivity and purity, suitable for large-scale industrialized production, and the like.

The invention discloses an experimental method for treating mastitis by using pedunculoside. The experimental method specifically comprises the following steps: S1, selecting ICR mice 3-7 days after childbirth as experimental objects in the experiment; and dividing the ICR mice into five groups, namely a blank control group (NT), an administration group (PE), a mastitis model group (LPS), a drug therapy group (LPS+PE) and a dexamethasone positive control group (LPS+DEX), wherein each group comprises 9 mice; and S2, anesthetizing and fixing the mice by using pentobarbital sodium (45mg / kg), injecting LPS (0.2 mg / kg) into a fourth pair of nipples of the mice to construct a mastitis model, and collecting a sample after 24 hours. The invention discloses an experimental method for treating mastitis by using pedunculoside. Pathological damage conditions of mammary gland tissues are detected by a paraffin section method, expression conditions of inflammatory genes IL-1[beta] in the mammary gland tissues are evaluated by a fluorescent quantitative PCR technology, and experimental results show that pedunculoside can effectively treat LPS-induced mastitis.

The invention discloses an application of pedunculoside in preparing a medicine for treating cerebral ischemia disease. An effective treatment dose of pedunculoside and a pharmaceutically acceptable carrier are prepared into the medicine for treating the cerebral ischemia disease, and the medicine can be pills, oral solution, injection, infusion agents, freeze-dried powder, microcapsules, capsules, or tablets. The medicines can be used for preventing and treating diseases such as apoplexy, cerebral infarction and the like.

The invention discloses application of pentacyclic triterpene compounds to preparation of medicine for treating adiposis and weight losing functional food. The pentacyclic triterpene compounds comprise pedunculoside and rotundic acid. The compounds have higher inhibition effect on lipase. Compared with synthetic medicine of Orlistat, the pentacyclic triterpene compounds have the advantages that the effect is mild; no irritation effect exists on the gastrointestinal tracts; the medicine is suitable for being taken for a long time.

The invention discloses the comprehensive use of an ovateleaf holly bark medicinal material, which relates to method for extracting and separating from plants and use in field of medicines, in particular to a method for extracting syringin, pedunculoside, ovateleaf holly bark acid and ovateleaf holly bark total saponins from an ovateleaf holly bark medicinal material and use. In the invention, the syringing is prepared from water extract solution of the ovateleaf holly bark medicinal material, the pedunculoside and ovateleaf holly bark acid are prepared from medicine slag obtained after waterextraction from the ovateleaf holly bark medicinal material, the purities of the syringin, pedunculoside and ovateleaf holly bark acid are all over 98 percent, and the ovateleaf holly bark total saponins are extracted from the medicine slag obtained after the water extraction from the ovateleaf holly bark medicinal material. The ovateleaf holly bark total saponins can be used for preparing medicines for preventing and treating hyperlipemia, medicines for relieving myocardial ischemia and angina and medicines for treating inflammation and pain. The invention has the advantages that: four active ingredients can be obtained from the ovateleaf holly bark medicinal material and resources can be fully utilized; and 2, the raw materials are readily available, the process is simple, the quality is controllable and batch production can be performed.

The invention relates to the technical field of medicine, and relates to a medical use of pedunculoside, in particular to application of the pedunculoside which is a natural source monomer compound topreparation of a colorectal cancer drug. The minimum effective dose of the pedunculoside is: 20 mg / kg. The pedunculoside can be prepared into a pharmaceutical composition with a pharmaceutically acceptable carrier, and the pedunculoside or the composition thereof can be prepared according to the conventional method in the art and can be applied to an enteric or parenteral or local way, includingoral administration, hypogloeeis, percutaneous administration, transmuscular or subcutaneous administration, skin mucosas, veins, urethra, vagina and the like.

The invention provides an application of pedunculoside: pentacyclic triterpene glycoside compound in preparing a diabetic skin ulcer drug.Pedunculoside has the effects of promoting the proliferationsof fibroblast and vascular endothelial cell and promoting vascular endothelial cell migration so as to effectively prompt the healing of skin ulcers in diabetic rats, and can be used for treating diabetic skin ulcers.In addition, the invention also provides a pharmaceutical composition for treating diabetic skin ulcers; the pharmaceutical composition contains pedunculoside or a pharmaceutically acceptable salt thereof, and can be made into various dosage forms to cope with diabetic skin ulcers of different types and different disease stages.

The invention discloses an ilicis rotundae cortex medicament composition for preventing such diseases as prostatitis and prostatic hyperplasia. The composition is obtained through extraction from barks of llex rotunda thunb. and refinement and is characterized by being formed by pedunculoside, syringin, rotundic acid and other substances. Pharmacological tests prove that the composition has the effect of preventing prostatitis, prostatic hyperplasia and the like. The composition has new application to preparing medicaments for preventing prostatic diseases. Pharmaceutic preparations of the composition are elaborated and can be any one of tablets, capsules, pills, granules, suspensions, dripping pills, ointments and rectal administration preparations such as suppositories.

The invention discloses an injection grade ovateleaf holly bark extract and a preparation method thereof, and a compound holly bark injection as well as a preparation method and the application thereof, and belongs to the field of traditional Chinese medicinal extracts and application. The preparation method of the injection grade ovateleaf holly bark extract is characterized by comprising the following steps: selecting a ovateleaf holly bark raw material and crushing into an ovateleaf holly bark crude powder; taking the ovateleaf holly bark crude powder, adding water for soaking, adjusting the pH value and adding enzyme for insulating; then adding ethanol, carrying out heating reflux extraction and filtering; recovering the ethanol from filtrate and filtering; taking the filtrate and adding n-butanol for extraction, taking a n-butanol liquid, adding water for shaking, discarding a water layer and recovering the n-butanol to obtain concentrate; adding methanol into the concentrate fordissolving, adding ether for precipitation, filtering and taking precipitate for drying to obtain the injection grade ovateleaf holly bark extract. The injection grade ovateleaf holly bark extract disclosed by the invention has the beneficial effects that the contents of syringin and pedunculoside are high; the compound holly bark injection disclosed by the invention has good effects in the application of treating yellow and white scour of piglets.

The invention provides an application of pedunculoside: pentacyclic triterpene glycoside compound in preparing a diabetic skin ulcer drug.Pedunculoside has the effects of promoting the proliferationsof fibroblast and vascular endothelial cell and promoting vascular endothelial cell migration so as to effectively prompt the healing of skin ulcers in diabetic rats, and can be used for treating diabetic skin ulcers.In addition, the invention also provides a pharmaceutical composition for treating diabetic skin ulcers; the pharmaceutical composition contains pedunculoside or a pharmaceutically acceptable salt thereof, and can be made into various dosage forms to cope with diabetic skin ulcers of different types and different disease stages.

The invention discloses an ilicis rotundae cortex medicament composition for preventing such diseases as prostatitis and prostatic hyperplasia. The composition is obtained through extraction from barks of llex rotunda thunb. and refinement and is characterized by being formed by pedunculoside, syringin, rotundic acid and other substances. Pharmacological tests prove that the composition has the effect of preventing prostatitis, prostatic hyperplasia and the like. The composition has new application to preparing medicaments for preventing prostatic diseases. Pharmaceutic preparations of the composition are elaborated and can be any one of tablets, capsules, pills, granules, suspensions, dripping pills, ointments and rectal administration preparations such as suppositories.

A pedunculoside extraction method adopting holly as raw material includes steps of extraction and purification. The extraction step includes performing refluxed extracting and isolating on the holly raw materials, water and ethanol solution under condition of microwave at low temperature ranging from 10 DEG C to 20 DEG C to obtain extracting liquid, and concentrating to obtain pedunculoside extract; dissolving the pedunculoside extract by 70-75% ethanol, filtering through activated carbon with 100 meshes, collecting filtrate and adjusting solution PH to be equal to 6 to 7, adding NaCl to saturate, cooling to minus 4 DEG C to 10 DEG C to precipitate, filtering and collecting precipitate, and drying to obtain high-purity pedunculoside.

The invention provides an application of an ilextrotunda thunb extract in preparation of a drug for promoting sleep. The Ilextrotunda thumb is used as a pharmaceutical plant with a wide range of medicinal uses, its extracts, especially a total saponin extract, more preferably, the pedunculoside in the total saponin extract can significantly improve insomnia caused by pentobarbital sodium in aspectof sleep promotion, can you shorten the sleep time, but can also extend the sleep time. The present application provides a novel purpose of the ilextrotunda thunb extract in promoting sleep, and provides a new selection for the current preparation of a sleep-promoting drug.

The invention relates to a preparation method of pedunculoside. The method includes the following steps of using the traditional Chinese medicinal material ovateleaf holly bark as the raw material, conducting heating reflux extraction through a 20%-70% ethyl alcohol aqueous solution, concentrating filtrate till no alcohol smell exists to obtain extract, dissolving and evenly mixing the extract in a 30%-70% ethyl acetate aqueous solution, standing overnight, conducting filtering to obtain filter residues, and drying the filter residues to obtain pedunculoside. Compared with the prior art, the method has the advantages of being simple in process, short in period, low in cost, high in product productivity and purity, suitable for large-scale industrialized production, and the like.

The invention discloses a traditional Chinese medicine for dental surgery anesthesia and a preparation method thereof. The traditional Chinese medicine includes the following raw materials: calmus, rhizoma nardostachyos, radix angelicae pubescentis, pentapetes phoenicea, glaucescent fissistigma root, typhae pollen carbo, passion flower, corydalis tuber, rhizoma drynariae, large-leaved gentian, stellaria media, purslane, herb of pedunculoside, radix angelicae, cuttlebone, hemiphragma heterophyllum, rhaphidophora decursiva, cryptotaenia japonica, dye yam, ivy tree bark, fruit of kwangsi dutchmanspip, catmint, symplocos chinensis, Japanese cayratia herb and leonurus pseudomacranthus. The invention has the following benefits: the traditional Chinese medicine for anesthesia utilizes China's traditional Chinese medicine theory, is nonpoisonous, has no side effects, and has the advantages of good pain killing and blood blocking effects, quick effect and low cost; when the traditional Chinese medicine is in use, no injection is required, and all that is needed is to use dental tweezers to grasp a cotton ball to bring medicine drops into or wipe the periodontal pocket of a tooth to be extracted multiple times; when no pain is felt at the periodontal tissues, a dental surgery is implemented. The traditional Chinese medicine for dental surgery anesthesia is capable of eliminating patients' sense of fear, and is particularly suitable for children and patients who are afraid or sick of injection.