The invention discloses an experimental method for treating
mastitis by using
pedunculoside. The experimental method specifically comprises the following steps: S1, selecting
ICR mice 3-7 days after
childbirth as experimental objects in the experiment; and dividing the
ICR mice into five groups, namely a blank control group (NT), an administration group (PE), a
mastitis model group (LPS), a
drug therapy group (LPS+PE) and a
dexamethasone positive control group (LPS+DEX), wherein each group comprises 9 mice; and S2, anesthetizing and fixing the mice by using pentobarbital
sodium (45mg / kg), injecting LPS (0.2 mg / kg) into a fourth pair of nipples of the mice to construct a
mastitis model, and collecting a sample after 24 hours. The invention discloses an experimental method for treating mastitis by using
pedunculoside.
Pathological damage conditions of
mammary gland tissues are detected by a
paraffin section method, expression conditions of
inflammatory genes IL-1[beta] in the
mammary gland tissues are evaluated by a fluorescent quantitative PCR technology, and experimental results show that
pedunculoside can effectively treat LPS-induced mastitis.