67 results about "Dioxygenase" patented technology

The invention discloses a cephalotaxus hainanensis Ch2OGD1 gene and application thereof, and belongs to the technical field of gene engineering. The Ch2OGD1 gene is used for coding a 2-oxoglutaric acid dependent dioxygenase (2OGD) type Pictet-Spengler enzyme, and the Pictet-Spengler enzyme is used for coding a Pictet-Spengler enzyme. Through a tobacco transient expression system and in-vitro enzyme activity determination, the Ch2OGD1 is proved to be capable of specifically catalyzing the condensation of dopamine and 4-hydroxyphenylpropanal to generate 1-phenethyl isoquinoline. The invention provides a directional preparation method of 1-phenethyl isoquinoline based on the Ch2OGD1 gene, provides a key enzyme element for biosynthesis of a cephalotaxine alkaloid precursor compound, and provides a technical basis for expanding the application of the gene in plant genetic engineering. And a potential technical path is provided for relieving the problem of resource shortage of cephalotaxine alkaloids.

The invention belongs to the technical field of biology, and particularly relates to a pentachloronitrobenzene rapid detection method based on enzyme catalysis reaction, which comprises the following steps: converting pentachloronitrobenzene into pentachlorophenol and nitrate radicals through an enzyme system consisting of iron thioflavin protein reductase, ferredoxin and nitrobenzene dioxygenase; and then nitrate radicals are specifically detected by a Griess reagent method so as to indirectly reflect quintozene. The detection method disclosed by the invention is good in specificity, short in detection time and remarkable in visual detection effect, and can be used for on-site rapid detection of quintozene.

Disclosed herein is a molecule, or a pharmaceutically acceptable salt thereof, that has a formula M1DO1-L-ME3, M1DO1 is a moiety that binds to IDO1, L is a linker covalently attaching M1DO1 and ME3, and ME3 is a moiety that binds to an E3 ubiquitin ligase. Disclosed herein are also the uses of the molecule, or a pharmaceutically acceptable salt thereof, and a pharmaceutical composition comprising the same, in a method of treating cancer in a subject in need thereof.

Various methods and compositions of treating 4-hydroxyphenylpyruvate dioxygenase-like (HPDL)-related diseases or disorders are presented herein. Also presented herein are methods of increasing CoQ10 biosynthesis, and methods of determining whether a subject will benefit from a CoQ10 or CoQ10 alternative treatment. Also presented herein are pharmaceutical compositions and dosage forms comprising 4-hydroxymandelic acid (4-HMA), and / or its metabolites. Further presented herein are compounds that inhibit 4-hydroxyphenylpyruvate dioxygenase-like (HPDL). Further presented herein are methods of identifying and / or assessing modulators of HPDL. Yet further presented herein are example methods and systems for isotopic labelling in cells by metabolizing cells in the presence of gaseous isotopic tracer.

The present application belongs to the technical field of quorum quenching enzyme preparation, and particularly relates to a multi-target quorum quenching enzyme preparation and a preparation method and application thereof, such as being mainly used for inhibiting the virulence factors of Pseudomonas aeruginosa, and including acyl homoserine lactone acyltransferase AiiO protein and 3-hydroxy-4-oxoquinolone 2,4-dioxygenase AqdC protein, and the mass ratio of the two is 20:(1-25); such as being mainly used for inhibiting the virulence factors and the synthesis of biological membranes of Pseudomonas aeruginosa, and further including deferiprone, and the mass ratio of AiiO, AqdC and deferiprone is 20:(1-25):(0.695-2.78), the present application can target the Las, Rhl and PQS of Pseudomonas aeruginosa, the yield and stability of the prepared AqdC protein are both high, the two types of enzymes are used in combination after the regulation amount, and the virulence factors regulated by different quorum sensing pathways in Pseudomonas aeruginosa are effectively inhibited; the regulation amount of deferiprone is used in combination with the two types of quorum quenching enzymes, and the virulence and biological membranes of Pseudomonas aeruginosa can be effectively inhibited in a comprehensive manner.

Provided are methods for treating cancer by administrating to an individual who has the cancer an inhibitor of 4-hydroxyphenylpyruvate dioxygenase (HPPD) and an immune checkpoint inhibitor. Also provided are methods for promoting translation of proteins within cells such that proteins include one or more misincorporated amino acids to thereby develop new proteins that contain neoantigens. Vaccines that include the neoantigens are included. Methods for producing and detecting misincorporation of a tyrosine in proteins expressed by cells are also provided.

This disclosure provides a method for treating a cancer patient comprising administering to the patient a therapeutically effective amount of an anti-PD-1 antagonist, for example, an anti-PD-1 or anti-PD-L1 antibody, in combination with an indolamine 2,3-dioxygenase inhibitor, wherein the patient is identified as exhibiting a combined biomarker comprising (a) a high IFNγ inflammatory signature score and (b) a low tryptophan 2,3-dioxygenase 2 (TDO2) gene expression score. The high IFNγ inflammatory signature score is determined by measuring the expression of a panel of IFNγ-related inflammatory genes in a cancer sample obtained from the patient, wherein the gene panel comprises, for example, IFNγ, CXCL10, CXCL9, HLA-DRA, IDO1, and STAT1. In some respects, the gene panel also includes CCR5, CXCL11, GZMA, and PRF1.In some aspects, the genetic panel comprises CXCR6, TIGIT, PD-L1, PD-L2, LAG3, NKG7, PSMB10, CMKLR1, CD8A, IDO1, CCL5, CXCL9, HLA.DQA1, CD276, HLA.DRB1, STAT1, HLA.E and TDO2.

Methods and systems for cell-free bioproduction of retinal and retinol are provided, which may be performed using a combination of a beta-carotene 15,15'-dioxygenase and an alcohol dehydrogenase. Cell-free retinoid production enables reaction rates and productivity not possible / not obtained in cell-based systems.

The invention relates to the technical field of biology, in particular to a tobacco-sourced carotenoid splitting dioxygenase compound enzyme preparation and application thereof. Three gene sequences for encoding carotenoid cleavage dioxygenase are identified from tobacco, and the compound enzyme preparation is prepared from the enzymes and can remarkably enhance the aroma amount and the aroma quality of tobacco leaves.

The disclosure provides a method for treating a subject afflicted with a cancer comprising administering to the subject a therapeutically effective amount of an anti-PD-1 antagonist, e.g., an anti-PD-1 or anti-PD-L1 antibody, in combination with an indoleamine 2,3-dioxygenase inhibitor, wherein the subject is identified as exhibiting a combined biomarker comprising (a) a high IFNγ inflammatory signature score and (b) a low tryptophan 2,3-dioxygenase 2 (TDO2) gene expression score. The high IFNγ inflammatory signature score is determined by measuring the expression of a panel of IFNγ related inflammatory genes in a cancer sample obtained from the subject, wherein the gene panel comprises, e.g., IFNγ, CXCL10, CXCL9, HLA-DRA, IDO1, and STAT1. In some aspects, the gene panel further comprises CCR5, CXCL11, GZMA, and PRF1. In some aspects, the gene panel comprises CXCR6, TIGIT, PD-L1, PD-L2, LAG3, NKG7, PSMB10, CMKLR1, CD8A, IDO1, CCL5, CXCL9, HLA.DQA1, CD276, HLA.DRB1, STAT1, HLA.E, and TDO2.

The invention discloses a mutant of aromatic dioxygenase CpuADO and application of the mutant. The amino acid sequence of the mutant of the aromatic dioxygenase CpuADO is as shown in SEQ ID NO: 2 or SEQ ID NO: 3. The aromatic dioxygenase CpuADO is found to have broad-spectrum substrate conversion capacity and can catalyze various lignin monomer derived aromatic olefin substrates to generate corresponding aromatic aldehyde or aromatic ketone products, and the catalytic efficiency of two mutants obtained after site-directed mutagenesis of the aromatic dioxygenase CpuADO is further improved; the problems that existing aromatic dioxygenase is poor in adaptability to lignin monomer derived aromatic olefin substrates, narrow in substrate spectrum and low in catalytic efficiency are solved, high-valued utilization of multiple types of lignin monomer derived aromatic olefins is facilitated, and an efficient novel enzyme tool is provided for green biological oxidation conversion of lignin monomer derived aromatic compounds.

The application discloses a Talaromyces with multi-oxidase synergistic expression capacity and a fermentation method and application thereof. Talaromyces sp. The Talaromyces (Talaromyces autumnalis) Ta-lac is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC No. 42125. Compared with the engineering strain for biosynthesis of laccase, the strain has strong environmental adaptability, can naturally synthesize laccase, and has relatively stable genome, no potential risk of exogenous genes and relatively high safety. The Talaromyces has a short growth cycle, high yield of laccase, nitropropane dioxygenase, hydrogenase coenzyme dioxygenase and squalene monooxygenase and high lignin degradation activity in a fermentation process. The Talaromyces Ta-lac is applied to degradation of toxin dye wastewater, and has remarkable detoxification and decolorization effects.

The invention provides a method for producing beta-hydroxy-beta-methylbutyric acid through double-enzyme co-expression, and relates to the technical field of enzyme engineering. According to the invention, L-amino acid deaminase and 4-hydroxyphenylpyruvate dioxygenase are expressed by using the same promoter in a host cell, and a key RBS combination is determined by optimizing and screening ribosome binding site RBS sequences, so that expression balance and efficient synergy of the two enzymes are realized; on the basis, biosynthesis of beta-hydroxy-beta-methylbutyric acid is carried out by taking L-leucine as a substrate, the yield of beta-hydroxy-beta-methylbutyric acid reaches 14.12 mM, and the molar conversion rate is 56.5%. Through series co-expression design of double enzymes, efficient biosynthesis of beta-hydroxy-beta-methylbutyric acid is achieved, and the method has the advantages of being mild in reaction condition, high in product purity, environmentally friendly and the like and is suitable for industrial popularization.

Provided are a herbicidal composition and a method for controlling weeds that have a superior weed controlling effect. The present herbicidal composition comprises one or more uracil compounds selected from the group consisting of a compound represented by formula (I) and a compound represented by formula (II), and one or more compounds selected from the group consisting of the herbicide compound group B and the safener group C, wherein a weight ratio of the uracil compounds to one or more compounds selected from the group consisting of the herbicide compound group B and the safener group C is 1:0.02 to 1:50, and the herbicide compound group B is the group consisting of the following B-1 to B-12 below: B-1, acetolactate synthase inhibitors; B-2, acetyl CoA carboxylase inhibitors; B-3, protoporphyrinogen IX oxidase inhibitors; B-4, 4-hydrophenylpyrubic acid dioxygenase inhibitors; B-5, phytoene desaturase inhibitors; B-6, photosystem II inhibitors; B-7, very-long-chain fatty acid synthesis inhibitors; B-8, microtubule formation inhibitors; B-9, auxin-type herbicides; B-10, enolpyruvylshikimate-3-phosphate synthase inhibitors; B-11, glutamine synthase inhibitors; and B-12, other herbicides; (including agriculturally acceptable salts or derivatives thereof).

The invention discloses an isoleucine dioxygenase mutant, the amino acid sequence of which is obtained by mutating the amino acid sequence as shown in SEQ ID No.2, and discloses a technical method for synthesizing 4-hydroxyisoleucine through whole-cell multi-enzyme catalysis based on the mutant. Cheap L-isoleucine and L-sodium glutamate are used as raw materials, a whole-cell catalysis mode is adopted, 4-hydroxyisoleucine is asymmetrically catalyzed and synthesized under the mild condition, no chemical catalyst is needed, and the method has the advantages of being environmentally friendly, high in synthesis efficiency and the like and is a green biological catalysis synthesis approach.

According to the IDO1 activity detection method provided by the invention, by adding exogenous tryptophan and optimizing experimental parameters such as the cell inoculation amount, the cell culture time and the IFN-gamma concentration, the activity of IDO1 enzyme generated by the mesenchymal stem cells is more accurately measured, so that the immune regulation and control capability of the mesenchymal stem cells is more accurately evaluated.

This invention provides recombinant polynucleotides comprising nucleic acids encoding CYP76AD6 or related genes, and their use for the production of L-DOPA from tyrosine and for the treatment of dopamine-responsive disorders such as Parkinson's disease. This invention also provides recombinant polynucleotides comprising nucleic acids encoding CYP76AD1 and / or CYP76AD6, nucleic acids encoding DOPA 4,5-dioxygenase (DOD) such as beet DODA1, and in some cases, nucleic acids encoding betaine-related glucosyltransferases such as the four o'clock gene cyclic-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for the production of betaine. Finally, this invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms (including plants), and their use in the various methods of this invention.

The present disclosure relates to recombinant DNA molecules and engineered proteins useful for conferring tolerance to β-triketone herbicides such as mesotrione. The present disclosure also provides herbicide tolerant transgenic plants, plant parts, cells and seeds comprising the recombinant DNA molecules, and methods of using the same.

This invention discloses a *Aspergillus ryukyu* strain and its applications. The *Aspergillus ryukyu* strain is named *Aspergillus ryukyu* P001, with accession number CGMCC NO.42119, deposited on July 3, 2025, at the China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. The *Aspergillus ryukyu* strain provided by this invention can efficiently secrete catechol 1,2-dioxygenase, significantly improving the degradation efficiency of phenolic substances. When the strain provided by this invention or its produced catechol 1,2-dioxygenase ferments tea (such as ripe Pu-erh tea), it can accelerate the degradation of tea polyphenols, catechins, and other substances, promote the formation of theaflavins, resulting in a reddish-brown tea liquor with a mellow taste and a shortened fermentation cycle.

This invention discloses an enzyme for preparing C7-hydroxylated steroidal compounds, its gene, recombinant vector, engineered bacteria, and applications, belonging to the field of synthetic biotechnology. It addresses the problems of low expression levels and low conversion rates in the enzymatic conversion of BA to 7-OH-DBA. The amino acid sequence of the enzyme is shown in SEQ ID NO: 2. This invention also provides the gene sequence of this enzyme and a recombinant genetically engineered bacterium expressing the C7-hydroxylase. The enzyme of this invention is modified from an α-ketoglutarate-dependent dioxygenase. Compared with the wild type, the modified enzyme obtained in this invention can be successfully applied to the conversion of BA, achieving a conversion rate of 75% to produce 7-OH-DBA. This invention achieves enzymatic catalytic C7-hydroxylation of BA, which, compared with traditional chemical synthesis methods, is simpler, greener, and more economical. The synthesis of 7-OH-DBA will provide strong support for the development of new processes for steroidal drug production.

The invention discloses a ketoglutaric acid / Fe-dependent dioxygenase Chr12604 protein and an application of the ketoglutaric acid / Fe-dependent dioxygenase Chr12604 protein in degradation of TBBPA (Tetrabromo Benzoic According to the invention, the optimized method is utilized to carry out high-throughput excavation on molecular elements of microorganisms responding to the stress of the toxic and nondegradable organic pollutants, and important functional elements obtained by excavation are applied to monitoring of the toxic and nondegradable organic pollutants and transformation of chassis cells of a treatment technology.

The present invention relates to a mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a biologically active fragment thereof, and an isolated polynucleotide comprising a nucleic acid sequence encoding the protein or fragment thereof, wherein the mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a biologically active fragment thereof retains or enhances the ability to catalyze the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisic acid and is significantly less sensitive to HPPD-inhibiting herbicides than wild-type HPPD. The present invention also relates to nucleic acid constructs, expression vectors, and host cells comprising the polynucleotide, as well as methods for producing plants that have the ability to catalyze the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisic acid and are significantly less sensitive to HPPD-inhibiting herbicides.

This invention discloses a recombinant yeast for producing α-ionone, its construction method, and its applications. The construction method involves using a yeast encoding osmanthus flowers... Osmanthus fragrans The gene for the carotenoid cleavage dioxygenase OfCCD1 was obtained through rational modification and codon optimization. OfCCD1m The gene, with its 5' end fused to the N-terminal membrane-anchored peptide coding sequence of human tyrosine kinase LCK, was transformed into the δ site of Saccharomyces cerevisiae TW3 to obtain recombinant yeast TW4 producing α-ionone. The Saccharomyces cerevisiae strain TW4 producing α-ionone of this invention has the following beneficial effects: it achieves de novo synthesis of α-ionone in Saccharomyces cerevisiae for the first time, filling a technological gap in this field; by using a membrane anchoring strategy to locate the OfCCD1m enzyme in the yeast plasma membrane, the contact efficiency between the enzyme and the substrate ε-carotene is significantly improved, resulting in an α-ionone yield of 1.46 mg / L.

The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and / or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as Mirabilis jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.

Disclosed herein are compounds of formula (I) which are inhibitors of an IDO enzyme: Also disclosed herein are uses of the compounds in the potential treatment or prevention of an IDO-associated disease or disorder. Also disclosed herein are compositions comprising these compounds. Further disclosed herein are uses of the compositions in the potential treatment or prevention of an IDO-associated disease or disorder.

The application discloses a genetically engineered bacterium with high yield of gallic acid and application thereof, and belongs to the technical field of gene recombination.The genetically engineered bacterium with high yield of gallic acid is prepared from Yarrowia lipolytica ATCC MYA-2613 as a starting strain, and the ku70 gene is knocked out; the gene coding 4-hydroxyphenylpyruvate dioxygenase is expressed; the shikimic acid pathway is strengthened; and three copies of tHMGR and the lysine defect are integrated.In 2L fermenter fed-batch fermentation, the yield of gallic acid is 33.71g / L, which is more than 10 times higher than the highest yield of gallic acid of 2.8g / L of the genetically engineered Yarrowia lipolytica reported at present in 2L fermenter fed-batch fermentation, and the application lays a solid foundation for industrialization of Yarrowia lipolytica for producing gallic acid, and has an industrial application prospect.

Presently provided are inhibitors of cellularly expressed TD02 and pharmaceutical compositions thereof, useful for modulating an activity of tryptophan 2, 3 dioxygenase; treating immunosuppression; treating a medical conditions that benefit from the inhibition of tryptophan degradation; enhancing the effectiveness of an anti-cancer treatment comprising administering an anti-cancer agent; and treating tumor-specific immunosuppression associated with cancer.

The invention provides a 4-hydroxyphenylpyruvate dioxygenase mutant and application of the 4-hydroxyphenylpyruvate dioxygenase mutant in synthesis of beta-hydroxy-beta-methylbutyric acid, and relates to the technical field of enzyme engineering. According to the invention, molecular docking scoring is utilized to screen species sources of 4-hydroxyphenylpyruvate dioxygenase, then single-site and multi-site combined mutation is carried out, and when the 4-hydroxyphenylpyruvate dioxygenase (HPPD) mutant Q65L / E99D / V306I / L260I is obtained through screening and is co-expressed with L-amino acid deaminase (LAAD), the mutant Q65L / E99D / V306I / L260I can be used as a mutant of the 4-hydroxyphenylpyruvate dioxygenase. In the reaction of catalyzing L-leucine to generate beta-hydroxy-beta-methylbutyric acid, the catalytic efficiency and the product yield are remarkably improved, the problem that the efficiency of synthesizing HMB by an existing biological method is relatively low is effectively solved, and the method has important industrial application value.

A transgenic soybean event, Gm_CSM63714, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63714 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63714, and methods of using and detecting Gm_CSM63714 are also provided. Soybean plants containing the event Gm_CSM63714 exhibit tolerance to benzoic acid auxins such as dicamba; phenoxy auxins such as 2,4-D; inhibitors of glutamine synthetase such as glufosinate; and P-triketone inhibitors of 4-hydroxyphenylpyruvate dioxygenase (HPPD) such as mesotrione.