1991 results about "Oxidative stress" patented technology

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus rhamnosus capable of maintaining bone health and improving local joint inflammation and a metagen and application of the lactobacillus rhamnosus. According to the invention, the probiotic NKU ML1-2 is subjected to fermentation culture, such that a post-generation product is prepared. Results of embodiments prove that the metagen product can enhance the oxidation resistance of cartilage and osteoblasts and promote the synthesis of anti-inflammatory factors and cartilage matrixes (type II collagen and proteoglycan), and has the potential of relieving oxidative stress and inflammatory response of local joints, relieving joint inflammation and delaying cartilage destruction. The prepared metagen product is high in safety and good in stability, and can be applied to preparation of medicines for preventing and / or treating osteoarthritis; the metagen product can also be used for promoting bone health, enhancing the antioxidant capacity of osteoblasts and / or chondrocytes, promoting osteoblast activity and / or promoting calcium absorption and fixation, and a new solution is provided for joint health management.

The invention relates to the field of biological imaging, and particularly discloses an in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging, which comprises the following steps: S1, by using a two-photon frequency domain fluorescence lifetime imaging TP-FD-FLIM technology, losslessly representing microenvironment change through fluorescence lifetime parameters, and monitoring oxidative stress reaction and neutrophil migration behavior in real time, analyzing the effect of the associated compound in combination with an in-vitro cell model; and S2, based on the physical basis of FD-FLIM, establishing a frequency domain fluorescence lifetime signal processing and analysis system under periodic excitation, analyzing the dynamic correlation characteristics of the phase delay phi and the modulation depth M of a fluorescence signal relative to exciting light, and carrying out quantitative backstepping on molecular lifetime parameters. According to the in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging provided by the invention, neuroinflammatory response can be observed in a lossless and real-time manner on the subcellular scale.

The invention discloses an active oxygen scavenging drug-loaded targeted nano delivery platform based on diselenide bond bridging as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the invention, tetra (4-carboxyphenyl) porphyrin (TCPP) and manganese porphyrin (Mn-TCPP) are used as raw materials to synthesize an organic metal framework MOFs, a target drug is loaded on the MOFs through electrostatic adsorption / blending, and amino and diselenide bonds are linked on the MOFs to realize combination with a specific peptide ligand and promote release of a drug oxidative stress microenvironment. The nano delivery platform with specific targeting and ROS response performance is obtained. The specific treatment effect of different diseases is achieved through different selections of the loaded drugs and the specific peptide ligands.

The invention discloses a scylla paramamosain keep-alive and soft shell soft-keeping method, a low-temperature keep-alive mode refers to an on-ice keep-alive mode or a low-temperature acidification keep-alive mode, preferably, the low-temperature acidification keep-alive mode: cooling seawater to 16 DEG C or below by using a cooling-water machine, then adjusting the pH value of the seawater to be less than 8 to obtain low-temperature acidified seawater, and then cooling the low-temperature acidified seawater to obtain the scylla paramamosain soft shell soft-keeping method. And putting the soft-shell crabs which just molt into low-temperature acidified seawater for keeping alive and softness. The hardening of the soft shell can be obviously delayed by low-temperature acidified seawater and on-ice keep-alive; low-temperature acidified seawater and on-ice keep-alive soft-shell crabs can improve the delicate flavor and sweet taste of hepatopancreas of the soft-shell crabs, and the flavors of the two keep-alive soft-shell crabs are better than those of cold-stored dead soft-shell crabs; on-ice and low-temperature acidified seawater keep-alive interferes with glycolipid metabolism, oxidative stress and cell apoptosis of the soft-shell crabs, and compared with on-ice keep-alive, low-temperature acidified seawater keep-alive influences are milder than on-ice keep-alive.

The invention discloses a zinc polyphenol complex suppository as well as a preparation method and application thereof. The preparation process mainly comprises two steps as follows: a, preparation of EGCG-Zn metal polyphenol complex nanoparticles; and b, preparing the nano complex suppository. The method is simple in process, green, environment-friendly and suitable for large-scale preparation. The prepared EGCG-Zn nanoparticles are uniform in particle size and good in stability, and have good antioxidant, anti-inflammatory and tissue repair activity based on a complex network structure formed by self-assembly of EGCG and zinc ions. The nano-particles are firstly treated by adopting a drug pretreatment carrier and then loaded in a suppository matrix to construct a rectal administration type nano-suppository, so that targeted and continuous local drug effect release can be realized. In intervention of radioactive intestinal injury, the suppository not only can relieve oxidative stress and inflammatory response, but also can promote regeneration and repair of intestinal mucosa, and has good treatment potential and clinical application prospect.

The invention belongs to the field of nano-enzyme preparation and biological application, and particularly relates to a preparation method and application of M2 type macrophage membrane coated FeMn diatomic nano-enzyme. The preparation method comprises the following steps: by taking nitrogen-doped carbon (BANT) as a carrier, loading Fe and Mn diatoms, synthesizing a novel nano material FeMnDA / BCNT diatomic nano-enzyme, extracting a macrophage membrane from natural macrophages, and coating the FeMnDA / BCNT nano material with the macrophage membrane to finally form M2 type macrophage membrane coated nano-particles, namely [MM] FeMnDA / BCNT. The [MM] FeMnDA / BCNT nano-enzyme prepared by the invention has good biological safety and simulated SOD and CAT enzyme activity, and the expression of inflammatory factors is reduced by removing excessive ROS (reactive oxygen species) in the cartilage cells induced by H2O2, so that the damage of oxidative stress to the cartilage cells is inhibited. The [MM] FeMnDA / BCNT nano-enzyme is not used for treating OA yet, so that a scientific basis is provided for further application and expansion of the diatomic nano-enzyme, and an effective strategy and a new thought are provided for treating osteoarthritis and chronic diseases related to oxidative stress in the future.

The disclosure relates to methods, compositions, and combinations for detoxification in a patient comprising performing therapeutic plasma exchange (TPE) on the patient. The disclosure also relates to methods, compositions, and combinations for removing toxins in a patient as a result of chronic exposure to the toxins, comprising performing one or more TPE sessions on the patient and administering an oral composition to the patient. The methods, compositions, and combinations of the disclosure may also be used to replenish biomarkers indicative of a healthy state of the body in order to improve immune system function or patient longevity, and / or reduce oxidative stress or inflammation. The methods, compositions, and combinations of the disclosure may also be used to reduce the level of atherosclerosis, improve cognitive function, prevent early cancer development, promote immune health, and / or treat health conditions or diseases such as autoimmune diseases, cancers, Alzheimer's disease, Parkinson's disease, chronic fatigue syndromes, or fibromyalgia. Additionally, the methods, compositions, and combinations of the disclosure may comprise an intravenous supplementation, wherein each TPE session is followed by administration to the patient of an IV composition. The disclosure further relates to oral compositions and IV compositions for use in the methods, compositions, and combinations of the disclosure.

The invention discloses a therapeutic nano-vesicle preparation rich in mitochondrial functional protein, a preparation method of the therapeutic nano-vesicle preparation and application of the therapeutic nano-vesicle preparation in treatment of radioactive skin injury. The preparation method comprises the following steps: culturing, amplifying and cleaning umbilical cord mesenchymal stem cells, dispersing the umbilical cord mesenchymal stem cells in a suspension, mechanically extruding the umbilical cord mesenchymal stem cells into a crude solution through a multistage aperture polycarbonate membrane, and centrifuging, concentrating and purifying the crude solution to obtain the preparation. The preparation has the remarkable advantages that local administration can be realized, inflammation can be quickly inhibited, oxidative stress is reduced, and a repair pathway is activated, so that the preparation is suitable for treating acute radioactive skin injury; the preparation method is easy to standardize and scale, high in stability and convenient for emergency storage and delivery, and avoids the defects of cell therapy; a plurality of targets such as immune inflammation, oxidative stress and the like can be synergistically regulated, the biological effect of hUMSCs is simulated and partially replaced, and the limitation caused by the cell survival rate and the microenvironment dependency is avoided; the key function of hUMSCs is covered in function, and the clinical feasibility, safety and emergency suitability of the application level are higher.

The invention provides application of enhanced and optimized mesenchymal stem cells and exosomes in anti-aging or senescence delaying, and belongs to the technical field of biological medicine manufacturing. According to the pharmaceutical composition disclosed by the invention, the activity-enhanced mesenchymal stem cells and the exosomes secreted by the activity-enhanced mesenchymal stem cells are applied in a combined manner, so that a remarkable synergistic interaction effect is shown in anti-aging intervention. Experiments prove that the composition can systematically improve oxidative stress injury related to aging, significantly recover antioxidant enzyme activity of serum and brain tissue to be close to a normal level, and effectively reverse cognitive function degeneration, which is represented as behavioral improvement such as shortening of escape latency, improvement of spatial memory ability and the like; the invention is suitable for preparing drugs or functional preparations for delaying body aging or resisting aging, and provides an innovative technical path for developing an efficient and safe anti-aging therapy.

The invention discloses a mesenchymal stem cell membrane bionic nano-enzyme for targeted therapy of Parkinson's disease as well as a preparation method and application of the mesenchymal stem cell membrane bionic nano-enzyme. The mesenchymal stem cell membrane bionic nano enzyme comprises an inner core and a mesenchymal stem cell membrane, wherein the inner core is composed of mesoporous polydopamine nanoparticles, and the mesenchymal stem cell membrane coats the inner core. The medicine contains the mesenchymal stem cell membrane bionic nano enzyme. The mesenchymal stem cell membrane bionic nano-enzyme disclosed by the invention shows good biocompatibility, and can be homed to a Parkinson disease region in the brain under the mediation of CXCR4 and VLA-4 specific membrane proteins. In addition, through the CD47 membrane protein expressed on the surface, the removal of the immune system can be avoided, so that the action time in the body is prolonged. The nano-enzyme is helpful for eliminating reactive oxygen free radicals, reducing oxidative stress level and reducing damage of neuroinflammation to dopaminergic neurons, so that safe and efficient treatment of Parkinson's disease is realized.

The invention relates to morin nanoparticles, pH responsive hydrogel and application of the morin nanoparticles and the pH responsive hydrogel. The morin-loaded nanoparticles provided by the invention can be rapidly degraded in an alkaline environment, have certain stability in an acidic environment, have the capability of releasing morin in response to pH, and can realize accurate regulation and control of morin release according to the change of environmental pH. According to the invention, hyaluronic acid is further subjected to methacrylation treatment, such that MeHA is obtained. The characteristic that MeHA is easier to degrade in an acid environment is utilized, and MeHA is used as hydrogel to load CFMNPs and can be effectively used for treating wounds caused by burns and frostbite. Furthermore, the pH responsive double-layer hydrogel based on pigment nanoparticles-basic fibroblast growth factors can realize sequential release of the effective components according to the change of the pH value of the burn or frostbite wound. The composition can effectively inhibit inflammation, resist oxidative stress, promote proliferation and migration of endothelial cells, promote neovascularization and finally accelerate wound healing.

The invention discloses an application of phenyllactic acid or a composition containing phenyllactic acid in preparation of medicines for relieving lung diseases, and in the application, the lung diseases are preferably pulmonary fibrosis or acute lung injury. In an acute lung injury research model, phenyllactic acid can effectively intervene in the inflammatory factor cascade reaction and oxidative stress injury process, and acute lung injury symptoms caused by the factors are remarkably improved. Compared with the traditional treatment means, the experiment proves that the phenyllactic acid or the composition containing the phenyllactic acid can obviously relieve the symptoms of acute lung injury (ALI) of mice induced by lipopolysaccharide (LPS) and pulmonary fibrosis (PF) of mice induced by bleomycin, the pathological change of the lung is effectively improved, and the lung function is recovered.

The invention relates to the field of disease animal model construction, and particularly discloses a postmenopausal osteoporosis and knee osteoarthritis co-disease animal model construction method which comprises the following steps: selecting a female animal as an experimental subject; the selected female animals are injected with a GnRH agonist, meanwhile, low-calcium feed feeding and oxidative stress stimulation are conducted, and feeding is conducted for a preset time; detecting bone mineral density and bone trabecula parameters of the fed female animals, and screening qualified female animals meeting set standards; performing meniscus tearing operation on the qualified female animal, and implanting sustained release microspheres loaded with inflammatory factors at the torn part during the operation; carrying out weight-bearing training on the female animal after the operation, and continuing for a set time length; and performing health detection on the female animals at different time points. According to the method, through multi-factor collaborative induction of the PMOP, minimally invasive construction of the KOA and multi-dimensional health detection, accurate simulation of a co-disease pathological process and clinical characteristics is realized, and the authenticity and stability of the model are improved.

The invention discloses a preparation method and application of a novel hybrid extracellular vesicle. According to the invention, vesicles derived from endothelial cells and neutrophil cells are combined with deferoxamine, and a biological mixed nano vesicle platform (DFO (HEVS)) is developed to solve the problem of diabetes wound healing. According to the dual-targeting system, DFO is accurately delivered to a wound part by utilizing CXCR4 mediated endothelial cell homing and beta2 integrin dependent inflammation tropism, DFO (at) HEVs activates and recovers vascular regeneration through HIF-1alpha / VEGF, ferroptosis is inhibited through Nrf2 / GPX4 signal transduction, macrophages are reprogrammed into a repair promoting M2 phenotype, and oxidative stress-inflammation-ferroptosis circulation is effectively broken.

The invention discloses a magnesium-containing nanoparticle modified biological scaffold composite material as well as a preparation method and application thereof. The magnesium-containing nano particle modified biological scaffold composite material comprises a biological scaffold material and magnesium-containing nano particles modified on the biological scaffold material, the magnesium-containing nano particles comprise nano magnesium oxide particles and / or nano magnesium oxide-polyphenol compound particles, the nano magnesium oxide-polyphenol compound particles are of a shell-core structure, and the shell-core structure is of a hollow structure. Comprising magnesium oxide serving as a core and a polyphenol compound shell layer coating the surface of the magnesium oxide. The magnesium-containing nanoparticle-modified biological scaffold composite material provided by the invention can prolong the degradation and release of magnesium ions, promote improvement of microenvironment problems such as inflammation and active oxygen outbreak after spinal cord injury, regulate and control oxidative stress and inflammatory response, promote nerve regeneration and motor function recovery, and improve the survival rate of spinal cord injury. The problem that nanoparticles stop in a spinal cord injury area is effectively solved, and support is provided for migration, growth and differentiation of endogenous neural stem cells.

The invention relates to an anti-ultraviolet-damage composition and application thereof in sunscreen products, and the anti-ultraviolet-damage composition is prepared from the following raw materials in parts by weight: 5-8 parts of oxhide tea leaf extract, 3-7 parts of green alga extract, 1-4 parts of dogwood extract, 8-10 parts of sea-buckthorn extract, 2.5-4 parts of butyrospermum parkii butter and 5-8 parts of camellia oleosa seed oil. All the raw materials have a synergistic effect, and free radicals generated by ultraviolet induction are neutralized, so that oxidative stress is relieved, and skin cells are protected from being damaged; ultraviolet rays are absorbed and are prevented from penetrating through the skin, so that direct damage of the ultraviolet rays to the skin is reduced; the activity of tyrosinase is regulated or a melanin synthesis pathway is blocked, the accumulation of melanin is reduced, and the effects of preventing and improving stains, darkness and the like are achieved.

The invention discloses a spirulina extracellular vesicle-loaded astaxanthin hydrogel as well as a preparation method and application thereof, and astaxanthin is loaded into spirulina extracellular vesicles to form spirulina extracellular vesicle-loaded astaxanthin nanoparticles. And then, the nano-particles are contained in self-assembled hydrogel composed of aldehyde hyaluronic acid and carboxymethyl chitosan, and the spirulina extracellular vesicle astaxanthin-loaded hydrogel is prepared and obtained. The preparation method comprises the following steps: firstly, preparing the spirulina extracellular vesicle astaxanthin-loaded hydrogel, and preparing the spirulina extracellular vesicle astaxanthin-loaded hydrogel. The hydrogel prepared by the invention can realize synergistic and complementary effects of spirulina extracellular vesicles and astaxanthin, realizes a protective effect on radiodermatitis by relieving oxidative stress, recovering mitochondrial functions and reducing inflammatory response, and shows long-term biological safety.

The invention relates to a sodium hyaluronate fermentation filtrate as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. Through a two-stage fermentation mode, the fermentation filtrate containing sodium hyaluronate is prepared by recombining bacillus subtilis, and then residual nutrient substances in a culture medium are consumed by inoculating bacillus velezensis, so that the nutrient substances are metabolized into active substances with repairing and strengthening effects on hair; the prepared sodium hyaluronate fermentation filtrate has the effects of repairing scalp and strengthening hair. The sodium hyaluronate filtrate disclosed by the invention can be used for well reducing the content of NO in macrophages (the NO content of cells is increased due to malassezia lysate), can be used for removing active oxygen ROS of HaCaT cells and increasing the total tensile strength of hair, and has the potential effects of relieving scalp, resisting oxidative stress and enhancing the toughness of the hair.

The invention belongs to the technical field of nano-drugs, and discloses a multifunctional nano-drug delivery system for treating neovascular eye diseases and a preparation method of the multifunctional nano-drug delivery system. The nano drug delivery system disclosed by the invention is a composite nano preparation which takes dendritic mesoporous silica nanoparticles as a carrier and co-loads cerium zirconium oxide nano enzyme and an anti-VEGF (vascular endothelial growth factor) drug, various active oxygen can be efficiently removed, oxygen is continuously released through catalytic decomposition of hydrogen peroxide, a retina hypoxia microenvironment is improved, and the retina hypoxia effect is improved. According to the present invention, the anti-VEGF drug delivery system can reduce the oxidative stress induced retinal pigment epithelial cell apoptosis rate, reduce the inflammatory reaction, and simultaneously achieve the slow release and the controlled release of the anti-VEGF drug, and the drug effect maintenance time can achieve more than 60 days, such that the multi-target synergistic treatment can be achieved through the synergistic regulation of the multiple pathological links such as oxygen deficit-oxidative stress-inflammation-angiogenesis, and the anti-VEGF drug delivery effect can be achieved. The choroidal neovascularization can be effectively inhibited by single intravitreal injection, and a new strategy of multi-target collaborative treatment is provided for neovascular macular degeneration and other eye diseases.

The present invention provides an oriental cherry flower extract containing 200 mg / kg or more of neochlorogenic acid as an active ingredient. The oriental cherry flower extract provided by the invention can promote the degradation of saccharification products, and can improve cell oxidative stress or inflammatory response caused by the saccharification products.

The invention provides a culture method, application and detection method of mesenchymal stem cells for treating nervous system diseases, and belongs to the technical field of biological medicines. The invention provides a culture medium of mesenchymal stem cells, a method for culturing and subculturing the mesenchymal stem cells by using the culture medium, and a method for detecting the biological efficacy of the mesenchymal stem cells for treating nervous system diseases. The mesenchymal stem cell preparation disclosed by the invention has a relatively good treatment effect on nervous system diseases, and can be used for improving the new object recognition capability and the cerebral infarction range, inhibiting the release of inflammatory factors, reducing the oxidative stress level and narrowing the spinal cord lesion range so as to improve nervous system injury.

The invention provides an ovary-targeting ROS response type SS31 nano-particle and a preparation method and application thereof, and belongs to the field of biomedicine.The ovary-targeting ROS response type SS31 nano-particle is constructed by utilizing a porous metal organic framework to carry hyaluronic acid and utilizing an ROS response type thioketone linker to be combined with mitochondrial targeting peptide SS31, and the ovary-targeting ROS response type SS31 nano-particle is used for preparing the ovary-targeting ROS response type SS31 nano-particle. And the SS31 is targeted and delivered to the ovarian tissue. The nanoparticles target ovary and slowly release SS31 under the action of ROS, so that oxidative stress of oocytes and granular cells caused by female reproductive system diseases such as premature ovarian failure, polycystic ovarian syndrome and the like is reduced, and the mitochondrial function of the oocytes and the granular cells is improved; and a foundation is laid for the subsequent clinical treatment of diseases related to the mitochondrial dysfunction of the female reproductive system by using the SS31.

The invention discloses a feed additive for synergistically improving oxidative stress resistance of broiler chickens based on oleuropein-luteoloside-maslinic acid ternary combination and application of the feed additive, the feed additive is the olea europaea leaf extract, the total polyphenol content of the feed additive is about 52%, the total flavone content is 7%, and the total flavone content is 5%. The feed is mainly composed of oleuropein, luteolin-7-beta-glucoside and maslinic acid, the oleuropein, the luteolin-7-beta-glucoside and the maslinic acid are sequentially added in a molar ratio of 6: 1: 1 to generate a synergistic anti-oxidative stress effect, and when the feed is applied to breeding on the basis of corn-soybean daily ration of broilers, the daily gain of the broilers can be obviously increased, the feed conversion ratio is reduced, and the feed conversion rate is increased. The intestinal micro-ecological health, the anti-oxidative stress effect and the organism immunity are improved, and the infection rate and the death rate of intestinal diseases such as diarrhea of broilers are reduced. The product has the advantages of being simple and convenient to prepare and use, free of toxic and side effects, beneficial to broiler chicken growth and human health, free of the problems of drug resistance, drug residues and the like, and capable of promoting chicken meat quality improvement and the like.

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention discloses a dual-targeting macrophage membrane nano system and a preparation method and application thereof, and belongs to the field of biological medicine, MKA is wrapped in a macrophage membrane after AuNPs and mitochondrial targeting peptide KLA are connected. The preparation method comprises the following steps: firstly synthesizing AuNPs and modifying, and then wrapping with a macrophage membrane. In treatment, the compound can realize double targeting of tumor cells and mitochondria, enhance radiation-induced DNA damage, inhibit DDR, activate immune pathways and promote T cell infiltration, and can also consume glutathione and amplify oxidative stress to enhance immune effect, and the dose enhancement ratio of the compound in cooperation with 8GyX-rays reaches 3.1. In addition, the MKA is good in biocompatibility in vivo, long in blood circulation time, high in tumor targeting and capable of being quickly cleared through the kidney. The invention provides a new way for tumor radioimmunotherapy, and is helpful for promoting the progress of clinical tumor treatment.

The invention belongs to the technical field of exosome freeze-dried powder, and discloses exosome freeze-dried powder and a preparation method thereof, the exosome freeze-dried powder contains mesenchymal stem cell exosome, trehalose, sodium hyaluronate, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide. According to the exosome freeze-dried powder, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide are added, the membrane structure integrity of the exosome is effectively protected, the retention rate of active substances in the exosome freeze-dried powder is high, and therefore the effects of resisting inflammation, regulating cell metabolism, promoting tissue repair and the like of the exosome can be fully exerted. In addition, glutathione can directly remove skin free radicals and inhibit oxidative stress injury, and has an anti-oxidation effect; ceramide can also supplement skin lipid barrier, enhance the water locking capacity and enhance the moisturizing effect of sodium hyaluronate; fructo-oligosaccharide can also regulate skin micro-ecological balance, and has anti-inflammatory and bacteriostatic effects.

The invention relates to an anti-fatigue refreshing functional chewing gum containing natural active ingredients, which relates to the technical field of food, and comprises a gum base, a natural sweetening agent and an active ingredient mixture, the mustard extract and coffee are innovatively combined to form a dual-channel nerve activation mechanism, the mustard extract realizes instant refreshing through a targeted oral trigeminal nerve TRPA1 ion channel, caffeine maintains continuous excitation through antagonism of a central adenosine receptor, and the space-time cooperation of the mustard extract and the coffee breaks through the limitation of a single path. Meanwhile, a rhodiola rosea extract, a sea-buckthorn extract, an American ginseng extract and astragalus polysaccharide are compounded, so that a triple anti-fatigue physiological barrier with functions of nerve regulation, oxidative stress inhibition and energy metabolism optimization is formed. In the preparation process, a microcapsule embedding technology is adopted to control slow release of mustard active ingredients, menthol flavor is combined to synergistically trigger TRP channel dual activation, and unification of efficacy and palatability is achieved.

The invention provides lactobacillus plantarum AL4510, a microbial agent and application of the lactobacillus plantarum AL4510 to preparation of a medicine for relieving altitude stress and / or intestinal injury induced by a low-pressure and low-oxygen environment, and relates to the technical field of microorganisms. The lactobacillus plantarum AL4510 provided by the invention is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation date is March 10, 2025, and the preservation number is CCTCC NO: M 2025421. The lactobacillus plantarum AL4510 strain has a unique strong probiotic function of plateau lactic acid bacteria and relatively strong oxidation resistance, gastrointestinal fluid tolerance and safety, and can better relieve altitude stress and intestinal injury by reducing the oxidative stress level and inflammatory factors and regulating intestinal flora.