30results about How to "Increase secretion" patented technology

The invention discloses an anti-aging composition and a preparation method thereof, and belongs to the technical field of cosmetics, the anti-aging composition provided by the invention is prepared from the following components in parts by mass: 0.001-0.01 part of freeze-dried powder of secretin of porcine umbilical cord mesenchymal stem cells, 15-20 parts of argania spinosa leaf extract, 10-13 parts of eucheuma gelatinae extract and 1-3 parts of northeast stichopus japonicus adventitious root extract. The anti-aging composition provided by the invention has the effects of resisting aging and improving the migration rate of skin cells, and the whole anti-aging composition is mild and non-irritant; when the anti-aging composition is applied to cosmetics, the anti-aging composition can effectively resist aging, lighten wrinkles, improve skin elasticity and increase the water content of cuticles. In addition, the composition provided by the invention is natural in component source, simple in preparation method and suitable for actual production.

The invention discloses an induction culture medium for preparing human umbilical cord mesenchymal stem cell exosomes and application of the induction culture medium, and relates to the technical field of exosome culture. The additive is prepared from the following components: 0.8 to 1.5 percent of ITS, 0.03 to 0.05 percent of a luteolin extract, 0.04 to 0.06 percent of a herba taraxaci extract, 0.02 to 0.04 percent of a radix ginseng extract, 0.03 to 0.05 percent of resveratrol, 0.01 to 0.03 percent of vitamin C, 0.03 to 0.05 percent of a pimpinella thellungiana extract, 0.01 to 0.03 percent of a rhizoma dioscoreae extract and 0.01 to 0.02 percent of sodium citrate; the induction culture medium provided by the invention not only can avoid introduction of exogenous substances and reduce the pollution risk, but also can promote proliferation of stem cells and effectively induce synthesis and exosome secretion of a large number of human umbilical cord mesenchymal stem cells.

The invention provides a stem cell extract composition for promoting hair growth and a preparation method thereof, and belongs to the technical field of cosmetics. Fetal calf serum, dexamethasone, penicillin, streptomycin, interleukin-2 and magnetic composite particles are added into a DMEM culture medium to prepare the culture medium, the culture medium is used for in-vitro culture of stem cells, during culture, a stem cell solution is prepared by combining a low-frequency magnetic field with low-energy laser culture, exosomes are extracted from an extracting solution, the exosomes and the stem cells are mixed and embedded to prepare embedded microspheres, and the embedded microspheres are used for in-vitro culture of the stem cells. The stem cell extract composition for promoting hair growth is prepared by uniformly mixing the stem cell extract with extracts extracted from fresh ginger, ginseng and polygonum multiflorum, the storage stability of stem cells and exosomes is obviously improved, hair growth is promoted, hair follicle development is promoted, mouse hair is induced and maintained in a growth period, and the stem cell extract composition is free of hormone addition, safe and non-irritant, and has a good application prospect. Wide application prospects are realized.

The invention provides application of bifidobacterium adolescentis B-201 in preparation of products for relieving asthenopia and protecting eyesight, and belongs to the field of microbial fermentation. The bifidobacterium adolescentis B-201 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.27234, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 27, 2023. The strain is subjected to culture fermentation and freeze drying to obtain bacterial powder, after auxiliary materials are added, the bacterial powder can be used for improving eye symptoms, increasing tear secretion amount, reducing ocular surface disease index scores and prolonging short-distance continuous eye using time, and the strain can be used for preparing products for relieving asthenopia and protecting eyesight.

The invention provides a skin care composition containing stem cell extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The method comprises the following steps: mixing and culturing the extracted urine-derived stem cells and pilose antler stem cells in a DMEM culture medium added with epidermal growth factors, cholera toxin, penicillin, streptomycin, transferrin, IL-2 and IL-15, then separating a culture solution to obtain mixed exosomes, mixed stem cells and extracellular matrixes, preparing the mixed stem cells into stem cell lipidosome, and preparing the stem cell lipidosome. Embedding the stem cell extract and the mixed exosome into titanium dioxide / silicon dioxide microspheres, adding into an extracellular matrix, and uniformly dispersing to obtain the skin care composition containing the stem cell extract. The skin care composition containing the stem cell extract prepared by the invention can obviously prolong the preservation period of stem cells and exosomes and improve the repair and improvement of the stem cells and the exosomes on skin, and the prepared composition has better effects of resisting aging, whitening, promoting skin elasticity, resisting wrinkles and the like, and has a wide application prospect.

The invention belongs to the technical field of biology, and particularly relates to a small molecule peptide composition and method for regulating natural calculus bovis production. The small molecule peptide aiming at a key target spot is designed by deeply researching secretion and metabolism of ox bile. The action mechanism covers biliary secretion stimulation, component optimization, bezoar key enzyme activity promotion and immune response regulation. A chemical synthesis or biotechnology means is adopted for preparation, and the dosage is determined according to cattle conditions during application. The safety is strictly evaluated, the yield of the natural calculus bovis can be obviously improved by 65%, the quality is also greatly improved, the effective components are increased, the impurities are reduced, the drug effect is enhanced, a new efficient and safe way is opened up for the production of the natural calculus bovis, and the method has a wide application prospect.

This invention belongs to the field of plant extract technology, specifically relating to a plant extract composition for enhancing immunity. By weight, it is composed of the following raw materials: 3-5 parts Astragalus membranaceus extract, 2.5-4 parts Echinacea purpurea extract, and 1.5-3.5 parts Rhodiola rosea extract. This plant extract composition, through the scientific formulation of Astragalus membranaceus, Echinacea purpurea, and Rhodiola rosea, and an enzymatic extraction process, enhances the immunomodulatory effect. The combination of the three extracts increases the secretion of TNF-α and IL-6 by macrophages. Furthermore, the spleen lymphocyte stimulation index (SI) of the three extracts reaches 5.57, and the lymphocyte transformation rate increases from 191% to 457%. These results indicate that this plant extract composition effectively enhances the body's non-specific and cellular immune functions by activating macrophages and promoting T lymphocyte proliferation through multiple pathways, providing more comprehensive immune protection.

The present invention provides a novel pharmaceutical composition for treating dry eye, which can repair damaged cornea and increase tear secretion by significantly improving corneal damage and corneal opacity without side effects, thereby effectively treating ophthalmic diseases related to dry eye.

The invention belongs to the technical field of immunology, and particularly discloses application of 2 ', 3'-cGAMP in preparation of a medicine for enhancing chicken pox-herpes zoster virus vaccine immune response. The 2 ', 3'-cGAMP combined varicella-zoster virus vaccine disclosed by the invention can improve the level of a specific IgG antibody, enhance the cellular immune response intensity of a gE antigen and improve the secretion amount of IFN-gamma and IL-2, so that the immune response of the varicella-zoster virus vaccine is enhanced, the immunoprophylaxis effect is enhanced, and the 2 ', 3'-cGAMP combined varicella-zoster virus vaccine adjuvant can be used for preparing a vaccine adjuvant for treating the varicella-zoster virus. Good application prospects are realized.

The invention relates to a medicine for improving oral micro-ecology based on perilla leaves and application thereof, in particular to a medicine for improving oral micro-ecology based on perilla leaves and application thereof. The invention aims to solve the problem of human systemic diseases caused by oral harmful bacteria. The method comprises the following steps: crushing perilla leaves, adding distilled water or ethanol with a volume concentration of 50% according to a mass ratio of the perilla leaves to the distilled water or the ethanol with a volume concentration of 50% of 1: 20, soaking the distilled water in a closed container for 12 h, decocting with water for 3 h, and taking a filtrate; extracting with ethanol with the volume concentration of 50%, soaking in a closed container for 3 hours, performing ultrasonic extraction for 30 minutes, and taking filtrate; and repeatedly extracting the remaining medicines once, merging the filtrate, carrying out vacuum concentration on the filtrate, freeze-drying, preparing a solution with the medicine content of 1g / mL by using distilled water, and sterilizing by using a 0.22 mu m filter membrane to obtain the traditional Chinese medicine composition. The medicine for improving the oral micro-ecology based on the perilla leaves is used for improving oral mucosa immune globulin. Through the perilla leaf extract, the secretion amount of immune globulin in oral mucosa is increased, and the oral microenvironment is improved.

The invention discloses a preparation method and application of exosomes sourced from lactobacillus reuteri and bacillus subtilis for promoting cat hair degradation, the exosomes are respectively sourced from lactobacillus reuteri and bacillus subtilis cultured under a specific fatty acid condition, lauric acid (1-8g / L) is added into a lactobacillus reuteri culture medium, palmitic acid (1-8 g / L) is added into a bacillus subtilis culture medium, and secretion of exosomes is promoted by adjusting the metabolic pathway of thalli. The exosome obtained through extraction and mixing has biological activity of promoting degradation of hair protein in cats and accelerating decomposition of hair balls, and experimental results show that the exosome can significantly reduce the formation rate of the hair balls of the cats and the content of hair in excrement and improve the health state. The invention provides a novel microbial source functional component for developing a safe, efficient and drug-residue-free pet hair ball management product, and has a good application prospect and industrialization value.

The present invention relates to the technical field of recombinant enzymes. The present invention provides a phospholipase A1 and a method and application of its integrated secretory expression in Bacillus subtilis. The amino acid sequence of the phospholipase A1 is shown in SEQ ID NO.1. The present invention constructs various signal peptides and expression vectors combined from the Bacillus subtilis homologous protein signal peptide library, and combines a high-throughput screening method to screen out signal peptides that can improve PLA1 secretion, thereby optimizing the secretion of PLA1. Compared with the original signal peptide, the secretion amount of PLA1 is increased by 10 times, and the enzyme activity is increased by 10 times, which is of great significance for the reduction of PLA1 production cost and further large-scale application. In addition, the phospholipase A1 enzyme preparation prepared by the present invention can reduce the phosphorus content of rice oil to 70-75ppm at a temperature of 55°C and acidic conditions.

The present invention discloses a method and application for improving the production of cellulase by Trichoderma reesei, belonging to the field of biotechnology. The recombinant engineered Trichoderma reesei is obtained by introducing the exoglucanase gene LeGH7_3643 in shiitake mushroom into Trichoderma reesei for overexpression, and the nucleotide sequence of LeGH7_3643 is shown in SEQ ID NO: 1, or contains the gene sequence of the sequence shown in SEQ ID NO: 1. It is found through experiments that the activity of cellulase produced by the recombinant engineered Trichoderma reesei fermentation and the amount of protein secretion are significantly improved, and the enzyme liquid secreted by the recombinant engineered Trichoderma reesei can efficiently degrade different types of biomass raw materials, significantly improving the saccharification rate of biomass and the yield of ethanol.

The present invention proposes a fat stem cell composition for wound repair and a preparation method and application thereof, which belongs to the field of stem cell technology. Fetal bovine serum, dexamethasone, penicillin, streptomycin, collagen, magnetic particles, and substance P are added to DMEM culture medium to prepare a culture medium for low-frequency magnetic field culture of fat mesenchymal stem cells, separation, obtaining a stem cell solution, embedding to obtain stem cell microspheres, freeze-drying the supernatant to obtain paracrine culture material, and mixing with stem cell microspheres, hydroxypropyl methylcellulose, carboxymethyl chitosan, hyaluronic acid, glycerol, and stem cell microspheres to obtain a fat stem cell composition for wound repair, which has good effects of implanting damaged tissues, releasing nutrients, promoting new blood vessel formation, regulating oxidative stress and inducing anti-inflammatory reactions, and is helpful for wound repair. It can be converted into a gel phase at a skin epidermal temperature between 35-37°C, stabilizes components, and releases in a controlled manner.

The present invention provides microglia-derived exosomes and a preparation method and application thereof. The present invention provides a preparation method of microglia-derived exosomes, the preparation method comprising: culturing microglia in a culture medium containing gardenia glycosides, ligustrazine and puerarin to secrete exosomes, thereby obtaining microglia-derived exosomes. The microglia-derived exosomes prepared by the present invention can reduce neuroinflammation, inhibit oxidative stress and ferroptosis, reduce cerebral infarction volume, improve ischemia-reperfusion neurological scores, and improve brain tissue pathological damage, and have a neuroprotective effect on cerebral ischemia-reperfusion injury.

The present invention relates to a probiotic product for relieving diabetes and prediabetes, a preparation method and application thereof, comprising Lactobacillus plantarum AR113; or Lactobacillus plantarum AR113 and mulberry leaf extract. The probiotic product has the advantages that under the action of Lactobacillus plantarum AR113 or the synergistic action of Lactobacillus plantarum AR113 and mulberry leaf extract, diabetes can be better alleviated, and Lactobacillus plantarum AR113 also has a good effect on prediabetes by repairing pancreatic damage, reducing oxidative damage and liver glycolysis function, and can prevent and treat diabetes or prediabetes.

The invention discloses an induction culture medium for increasing the secretion amount of mesenchymal stem cells IL-10 and application of the induction culture medium, and belongs to the technical field of stem cell culture. In order to provide a method capable of improving the secretion amount of IL-10 of mesenchymal stem cells without influencing the proliferation of the mesenchymal stem cells, the method comprises the following steps: inducing amplified human mesenchymal cells by using a basic culture medium containing human IFN-gamma protein and TNF-alpha mimic peptide; and harvesting and culturing to obtain the human mesenchymal stem cells with increased IL-10 secretion amount. The induction culture medium provided by the invention can increase the secretion amount of mesenchymal stem cell IL-10 without influencing the proliferation of mesenchymal stem cells, and the mesenchymal stem cells obtained by induction are expected to be applied to the preparation of cell drugs for treating inflammatory diseases.

The present invention discloses a microneedle patch loaded with dacarbazine and manganese salt, a preparation method thereof, and an application in the treatment of melanoma, wherein the microneedle patch comprises a back-symmetric layer and a needle array arranged on the back-symmetric layer, wherein the back-symmetric layer and the needle array are both formed by an aqueous solution of a degradable polymer, and dacarbazine and manganese salt are loaded in the aqueous solution of the degradable polymer used to form the needle array. The drug-loaded microneedles obtained by the present invention can effectively inhibit the growth and metastasis of tumors, effectively prevent the recurrence of tumors, and achieve efficient killing of melanoma.

The present invention relates to a pharmaceutical composition for the treatment of neuroinflammation comprising eleutheroside B as an active ingredient, and more particularly, to a pharmaceutical composition for treating neuroinflammatory disease in patients with the increased activity of astrocytes or microglia, comprising eleutheroside B or a pharmaceutically acceptable salt thereof as an active ingredient. That is, it is possible to alleviate inflammation by effectively suppressing the activity of active microglia and astrocytes, increasing the secretion of anti-inflammatory cytokines, and to protect the damage of activated microglia and astrocytes to brain nerve cells.

CpG reduced nucleic acid variants encoding FVIII protein and methods of use thereof are disclosed. In particular embodiments, CpG reduced nucleic acid variants encoding FVIII are expressed more efficiently by cells, are secreted at increased levels by cells over wild-type Factor VIII proteins, exhibit enhanced expression and / or activity over wild-type Factor VIII proteins or are packaged more efficiently into viral vectors.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a culture medium for inducing mesenchymal cells to differentiate into insulin-secreting cells and application of the culture medium. By accurately regulating and controlling the oxidation-reduction state of NAD < + > / NADH in cells, the composition proportion of exogenous fatty acid, the dynamic supply concentration of glucose and the anti-oxidative stress level, the differentiation efficiency is remarkably improved, the induction period is shortened, and the method is suitable for industrial production on the premise of not depending on recombinant protein induction factors such as Activin A and Wnt3a. And a mature beta-like cell with stable glucose stimulus dependent insulin secretion (GSIS) capability is obtained. The kit is especially suitable for human adipose tissue-derived mesenchymal stem cells, and can be used as an independent kit product for preclinical research and drug screening of diabetes cell replacement therapy.

The application discloses application of a migration body in preparation of a medicine for inhibiting osteoclast differentiation or promoting osteogenesis. The application significantly improves the secretion amount of the migration body by optimizing cell culture conditions, realizes large-scale preparation, and greatly reduces production cost. The application also enriches active ingredients in the migration body by pressure stimulation, so that the migration body has more excellent effect of promoting bone defect repair. In addition, the migration body has larger size and a stable film structure, prolongs in-vivo retention time, realizes long-acting repair by single administration, and further solves the long-acting and clinical transformation problems. The migration body has wide application prospect in the field of bone defect repair.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a method for inducing mesenchymal stem cells to differentiate into insulin-secreting cells and application of the method. According to the method, a culture system is regulated and controlled by stages, the glucose concentration is respectively regulated in different induction stages, an NAD + precursor substance, a fatty acid composition and an antioxidant factor are added, and a three-dimensional microcarrier culture mode is combined, so that the cell metabolism state, the oxidation-reduction balance and the space structure are dynamically regulated and controlled. Under the condition of not depending on recombinant protein inducing factors such as Activin A and Wnt3a, the efficient directional differentiation of the mesenchymal stem cells to the insulin-secreting cells is realized. By adopting the method disclosed by the invention, the differentiation efficiency can be improved, the induction period can be shortened, the mature beta-like cells with a stable glucose stimulation dependent insulin secretion function can be obtained, and the method is suitable for induced differentiation and related research application of the human adipose-derived mesenchymal stem cells.

The present invention relates to a method for screening a cytokine having anticancer activity that inhibits the exosome secretion or suppresses the expression of PD-L1, wherein a vector composed of gene encoding cytokine, linker domain, and transmembrane domain transforms into cancer cells, and cytokine exhibiting anticancer activity can be selected, and thus the selected cytokine is provided as a new anticancer agent. In addition, the present invention relates to composition for preventing or treating cancer diseases, the composition comprising IL-15, selected using the above-mentioned screening technique, or expression promoter or activator thereof as an active ingredient. In the present invention, it was found that treating cancer cells with IL-15 suppresses exosome secretion and the expression of PD-L1, and it was also found that the anticancer effect is directly exhibited on the cancer cells. Therefore, IL-15 or expression promoter or activator can be effectively used to prevent or treat cancer diseases.

CpG reduced nucleic acid variants encoding FVIII protein and methods of use thereof are disclosed. In particular embodiments, CpG reduced nucleic acid variants encoding FVIII are expressed more efficiently by cells, are secreted at increased levels by cells over wild-type Factor VIII proteins, exhibit enhanced expression and / or activity over wild-type Factor VIII proteins or are packaged more efficiently into viral vectors.

It is an object of the present invention to provide a microorganism with improved protein secretion, and to provide a method for producing a protein using the aforementioned microorganism. According to the present invention, there is provided a microorganism, in which the expression of at least one ABC transporter ATP-binding protein is lost or decreased.

The application provides a kind of fermentation composition for assisting relieving asthenopia and its preparation method and application, belong to composition technical field.The fermentation composition is composed of the following components by weight:chrysanthemum 10-20 parts, medlar 10-20 parts, wine rhizoma polygonati 5-15 parts, radix codonopsitis 10-20 parts, fried cassia seed 5-10 parts and rose flower 5-10 parts.The application adopts the treatment method of "moistening water and nourishing yin, soothing liver and improving eyesight", and uses chrysanthemum, medlar, wine rhizoma polygonati, radix codonopsitis, fried cassia seed and rose flower, six kinds of food-medicine homologous traditional Chinese medicines for relieving asthenopia have good curative effect.

The invention discloses application of a stem cell exosome in treating acute lung injury, and belongs to the technical field of biology. The stem cell exosome is obtained by using human streptococcus pneumoniae lysate to stimulate umbilical cord mesenchymal stem cells. Compared with the conventionally cultured umbilical cord mesenchymal stem cell exosome, the secretion amount of the umbilical cord mesenchymal stem cell exosome can be remarkably increased by using the human streptococcus pneumoniae lysate to stimulate. When the exosome is used for repairing the LPS-induced human alveolar epithelial cell injury, the conventional exosome and the streptococcus pneumoniae lysate culture exosome with the same dosage can weaken the inhibition effect of the LPS on the activity of the human alveolar epithelial cell, and the streptococcus pneumoniae lysate culture exosome has a better repairing effect on the human alveolar epithelial cell injury than the conventional exosome.

There is provided a composition comprising: A) serine, glycine, betaine, N-acetylglycine, N-acetylserine, dimethylglycine, sarcosine and / or phosphoserine; B) N-acetyl cysteine, cysteine and / or cystine; C) carnitine, deoxycarnitine, gamma-butyrobetaine, 4-trimethylammoniobutanal, 3-hydroxy-N6,N6,N6-trimethyl-L-lysine, N6,N6,N6-trimethyl-L-lysine and / or lysine; and D) optionally nicotinamide riboside, quinolinate, deamino-NAD+, nicotinate D-ribonucleotide, nicotinamide D-ribonucleotide, nicotinate D-ribonucleoside, nicotinamide and / or nicotinate, wherein the molar ratio of A) to B) is between 16:1 and 1:4. The composition maybe used in a method of treatment of a medical condition selected from the group consisting of non-alcoholic fatty liver disease (NAFLD), alcoholic fatty liver disease (AFLD), type 2 diabetes, obesity, insulin resistance and dyslipidemia.

CpG reduced nucleic acid variants encoding FVIII protein and methods of use thereof are disclosed. In particular embodiments, CpG reduced nucleic acid variants encoding FVIII are expressed more efficiently by cells, are secreted at increased levels by cells over wild-type Factor VIII proteins, exhibit enhanced expression and / or activity over wild-type Factor VIII proteins or are packaged more efficiently into viral vectors.