8results about How to "Increase secretion" patented technology

This invention belongs to the field of plant extract technology, specifically relating to a plant extract composition for enhancing immunity. By weight, it is composed of the following raw materials: 3-5 parts Astragalus membranaceus extract, 2.5-4 parts Echinacea purpurea extract, and 1.5-3.5 parts Rhodiola rosea extract. This plant extract composition, through the scientific formulation of Astragalus membranaceus, Echinacea purpurea, and Rhodiola rosea, and an enzymatic extraction process, enhances the immunomodulatory effect. The combination of the three extracts increases the secretion of TNF-α and IL-6 by macrophages. Furthermore, the spleen lymphocyte stimulation index (SI) of the three extracts reaches 5.57, and the lymphocyte transformation rate increases from 191% to 457%. These results indicate that this plant extract composition effectively enhances the body's non-specific and cellular immune functions by activating macrophages and promoting T lymphocyte proliferation through multiple pathways, providing more comprehensive immune protection.

The invention discloses a preparation method and application of exosomes sourced from lactobacillus reuteri and bacillus subtilis for promoting cat hair degradation, the exosomes are respectively sourced from lactobacillus reuteri and bacillus subtilis cultured under a specific fatty acid condition, lauric acid (1-8g / L) is added into a lactobacillus reuteri culture medium, palmitic acid (1-8 g / L) is added into a bacillus subtilis culture medium, and secretion of exosomes is promoted by adjusting the metabolic pathway of thalli. The exosome obtained through extraction and mixing has biological activity of promoting degradation of hair protein in cats and accelerating decomposition of hair balls, and experimental results show that the exosome can significantly reduce the formation rate of the hair balls of the cats and the content of hair in excrement and improve the health state. The invention provides a novel microbial source functional component for developing a safe, efficient and drug-residue-free pet hair ball management product, and has a good application prospect and industrialization value.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a culture medium for inducing mesenchymal cells to differentiate into insulin-secreting cells and application of the culture medium. By accurately regulating and controlling the oxidation-reduction state of NAD < + > / NADH in cells, the composition proportion of exogenous fatty acid, the dynamic supply concentration of glucose and the anti-oxidative stress level, the differentiation efficiency is remarkably improved, the induction period is shortened, and the method is suitable for industrial production on the premise of not depending on recombinant protein induction factors such as Activin A and Wnt3a. And a mature beta-like cell with stable glucose stimulus dependent insulin secretion (GSIS) capability is obtained. The kit is especially suitable for human adipose tissue-derived mesenchymal stem cells, and can be used as an independent kit product for preclinical research and drug screening of diabetes cell replacement therapy.

The application discloses application of a migration body in preparation of a medicine for inhibiting osteoclast differentiation or promoting osteogenesis. The application significantly improves the secretion amount of the migration body by optimizing cell culture conditions, realizes large-scale preparation, and greatly reduces production cost. The application also enriches active ingredients in the migration body by pressure stimulation, so that the migration body has more excellent effect of promoting bone defect repair. In addition, the migration body has larger size and a stable film structure, prolongs in-vivo retention time, realizes long-acting repair by single administration, and further solves the long-acting and clinical transformation problems. The migration body has wide application prospect in the field of bone defect repair.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a method for inducing mesenchymal stem cells to differentiate into insulin-secreting cells and application of the method. According to the method, a culture system is regulated and controlled by stages, the glucose concentration is respectively regulated in different induction stages, an NAD + precursor substance, a fatty acid composition and an antioxidant factor are added, and a three-dimensional microcarrier culture mode is combined, so that the cell metabolism state, the oxidation-reduction balance and the space structure are dynamically regulated and controlled. Under the condition of not depending on recombinant protein inducing factors such as Activin A and Wnt3a, the efficient directional differentiation of the mesenchymal stem cells to the insulin-secreting cells is realized. By adopting the method disclosed by the invention, the differentiation efficiency can be improved, the induction period can be shortened, the mature beta-like cells with a stable glucose stimulation dependent insulin secretion function can be obtained, and the method is suitable for induced differentiation and related research application of the human adipose-derived mesenchymal stem cells.

CpG reduced nucleic acid variants encoding FVIII protein and methods of use thereof are disclosed. In particular embodiments, CpG reduced nucleic acid variants encoding FVIII are expressed more efficiently by cells, are secreted at increased levels by cells over wild-type Factor VIII proteins, exhibit enhanced expression and / or activity over wild-type Factor VIII proteins or are packaged more efficiently into viral vectors.

The application provides a kind of fermentation composition for assisting relieving asthenopia and its preparation method and application, belong to composition technical field.The fermentation composition is composed of the following components by weight:chrysanthemum 10-20 parts, medlar 10-20 parts, wine rhizoma polygonati 5-15 parts, radix codonopsitis 10-20 parts, fried cassia seed 5-10 parts and rose flower 5-10 parts.The application adopts the treatment method of "moistening water and nourishing yin, soothing liver and improving eyesight", and uses chrysanthemum, medlar, wine rhizoma polygonati, radix codonopsitis, fried cassia seed and rose flower, six kinds of food-medicine homologous traditional Chinese medicines for relieving asthenopia have good curative effect.

CpG reduced nucleic acid variants encoding FVIII protein and methods of use thereof are disclosed. In particular embodiments, CpG reduced nucleic acid variants encoding FVIII are expressed more efficiently by cells, are secreted at increased levels by cells over wild-type Factor VIII proteins, exhibit enhanced expression and / or activity over wild-type Factor VIII proteins or are packaged more efficiently into viral vectors.