The invention belongs to the field of biological
medicine, and discloses a
mesenchymal stem cell-derived
exosome and active factor
secretion composition and a directional preparation method thereof. According to the method,
umbilical cord tissues of healthy mammals are used as raw materials, primary stem cells are separated through enzymolysis, and amplification and passage are conducted to 3-5 generations through an alpha-MEM culture medium; a serum-free induction culture medium containing multiple functional components is adopted for directional induction, a conditioned culture medium is collected,
gradient centrifugation and
ultrafiltration concentration are performed, and the
protein concentration is calibrated to be 2 mg / mL, so that a finished product is obtained. The product can synergistically realize moisturizing, anti-
wrinkle and tightening effects. Specifically, the
exosome and active factor secreted composition is a composite
system which is obtained by separating and concentrating mesenchymal stem cells from a
conditioned medium of the mesenchymal stem cells after the mesenchymal stem cells are directionally induced by the method and is mainly composed of the
exosome, growth factors self-secreted by the stem cells,
cell factors and macromolecular
active protein.