43 results about "Umbilical cord tissue" patented technology

The present invention discloses an application of highly active human umbilical cord mesenchymal stem cells. HA-MSCs were isolated from human umbilical cord tissue using TeSR-E8 containing 5% CloneR and LN521-Coated culture flasks, and a technical system for HA-MSC isolation, expansion, and cryopreservation was established. Compared with conventionally cultured MSCs, the HA-MSCs prepared by the present invention have higher proliferation activity, smaller size, a higher nuclear-cytoplasmic ratio, and highly express embryonic stem cell-associated marker antigens SOX2, Nanog, and OCT4. They have the potential to differentiate into neural, myocardial, and hepato-intestinal progenitor cells derived from the three germ layers and possess excellent anti-aging activity.

The invention discloses a sleep-promoting nasal spray based on mesenchymal stem cell-derived exosomes and preparation and application thereof.The preparation method comprises the steps that 1, mesenchymal stem cells separated from umbilical cord tissue are cultured to P3-P5 generations, cell culture supernate is collected, cells are digested, and then cell debris and macromolecular impure protein are removed through pretreatment; (2) filtering and concentrating the pretreated cell culture supernatant and replacing a part of solution; (3) carrying out degerming filtration on the solution obtained in the step (2) through a 0.22 [mu] m filter membrane to obtain an MSC-Exos solution; and (4) adding a stabilizer with a proper concentration into the MSC-Exos solution to obtain a compound MSC-Exos solution, namely the sleep-promoting nasal spray. The product is a cell-derived natural active substance, can significantly improve sleep, and does not involve any pharmaceutical ingredient.

The invention discloses a skin-tightening anti-wrinkle essence and a preparation process thereof, and relates to the technical field of cosmetic preparation, and the preparation process comprises the following steps: obtaining animal umbilical cord tissue, culturing, collecting cell culture supernatant after culture, carrying out low-speed centrifugation to remove cells and dead cells, and carrying out concentration, filtration and sterilization through a tangential flow membrane technology to obtain the skin-tightening anti-wrinkle essence. The animal umbilical cord extract is obtained; the preparation method comprises the following steps: mixing the animal umbilical cord extract with water, butanediol, glycerol, hydroxypropyl tetrahydropyrantriol, 1, 2-hexanediol, trehalose, p-hydroxyacetophenone, allantoin, hydrolyzed sodium hyaluronate, sodium hyaluronate, tetrahydromethylpyrimidine carboxylic acid, xanthan gum and essence, homogenizing under a stirring condition, and standing for defoaming. By constructing an active stable microenvironment and an efficient transdermal delivery system, the technical problem that the animal umbilical cord extract serving as a core component is easy to inactivate and difficult to absorb is solved, and a faster and more stable skin structure remodeling effect is realized by utilizing the synergistic effect of the animal umbilical cord extract and functional components.

The present disclosure provides methods of treating Autism Spectrum Disorder. More particularly, the present disclosure relates to methods of using cord blood tissue-derived mesenchymal stromal cells (hCT-MSCs) to treat Autism Spectrum Disorder. The methods involve treating a patient having or suspected of having an Autism Spectrum Disorder by administering a therapeutically effective amount of hCT-MSCs.

The invention discloses hydrogel based on umbilical cord tissue and chorion tissue, which is prepared by a method comprising the following steps: (1) taking a placenta, shearing the umbilical cord and separating the chorion; (2) respectively carrying out tissue crushing on the umbilical cord and the chorion, and centrifugally collecting precipitates; mixing, washing, centrifuging and collecting precipitates; (3) freezing with liquid nitrogen, and then melting; repeatedly freezing and thawing; adding a buffer solution containing triton-X-100, and carrying out soaking treatment; rinsing, centrifuging and collecting precipitates; (4) adding urea, oscillating, centrifuging and collecting precipitate; freezing and drying to obtain freeze-dried powder; (5) dissolving the freeze-dried powder in hydrochloric acid, adding pepsin, and reacting; and adjusting the pH value to 7-7.5 to obtain the hydrogel. The hydrogel is applied to culture of cells, tissues or organoids, and is applied to preparation of a culture medium with the effect of promoting angiogenesis. The method is of great significance to development in the fields of regenerative medicine, disease modeling, medicine development and the like.

The invention relates generally to the fields of biology and health sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in amniotic membrane tissue and umbilical cord tissue preparations.

The invention relates generally to the fields of biology and health sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in amniotic membrane tissue and umbilical cord tissue preparations.

The invention provides an umbilical cord tissue cryopreservation solution, a cryopreservation method and a method for extracting umbilical cord mesenchymal stem cells. The freezing medium for the umbilical cord tissue comprises a basic permeable protective agent with the concentration of 5% v / v to 20% v / v, a non-permeable protective agent with the concentration of 5% v / v to 30% v / v, an antioxidant with the concentration of 0.1% v / v to 2% v / v, an ice control molecular material with the concentration of 0.001% v / v to 5% v / v, a buffer system with the concentration of 10 mM to 100 mM, an energy substance or antioxidant with the concentration of 0.1 mM to 10 mM, an ion carrier with the concentration of 0.1 mM to 5 mM and a membrane stabilizer. The cryopreservation solution for the umbilical cord tissue can ensure that cells in the umbilical cord tissue keep a survival state at low temperature, the preservation time of the cryopreservation solution is prolonged to several months and even one year, the preservation time of the umbilical cord is effectively prolonged, and the cryopreservation solution is far superior to an existing cryopreservation scheme.

The invention belongs to the technical field of cell culture, and particularly relates to a construction method of an immortalized goat umbilical cord mesenchymal stem cell line. The method comprises the following steps: by taking newborn goat lamb umbilical cord tissues as a research object, cleaning goat lamb umbilical cords, stripping blood vessels, separating Wharton's jelly, cutting the Wharton's jelly into 1mm < 3 > tissues, culturing by using a DMEM / F12 complete culture medium containing fetal calf serum, carrying out passage, cryopreservation, repeated resuspension and centrifugation, and inoculating into a culture dish for culture; lentiviruses containing SV40 large T antigens are packaged in vitro and are transduced into goat umbilical cord mesenchymal stem cells, and immortalization is realized after long-term subculture. The result of the embodiment shows that the construction method of the immortalized goat umbilical cord mesenchymal stem cell line provided by the invention is strong in repeatability, simple and efficient in operation steps, good in stem cell growth state and stable in physiological state.

The invention is applicable to the technical field of biological medicine, and provides a metformin-engineered umbilical cord mesenchymal stem cell and a preparation method and application thereof, and the preparation method comprises the following steps: taking fresh umbilical cord tissue, cleaning, cutting into small blocks, performing adherent culture, and performing fusion passage when the cell grows to 80%; and co-incubating the P5-generation human umbilical cord mesenchymal stem cells and metformin, and centrifugally collecting the cells after pancreatin digestion to obtain the metformin-engineered umbilical cord mesenchymal stem cells. The concentration of the metformin is 500 [mu] mol / L. Metformin is used for pretreating human umbilical cord mesenchymal stem cells to construct Met-MSC with metabolism enhancement and function optimization characteristics, so that the Met-MSC has stronger anti-apoptosis, anti-oxidation, angiogenesis promotion and immunoregulation capabilities, a safe, simple, convenient and convertible new cell treatment strategy is provided, and the application bottleneck of traditional MSC treatment in diabetic foot ulcer is broken through.

The application discloses a preparation method of a decellularized tissue nerve graft scaffold with electric conductivity, and belongs to the field of biomaterials. The method comprises the following steps: separating embryonic period umbilical cord tissue, removing cells and lipids, and then performing freeze-drying; a titanium carbide monolayer nanosheet solution is prepared by using a chemical etching method, a GelMA solution is prepared by using a photo initiator phosphate buffer salt solution, and the GelMA solution is mixed with the titanium carbide monolayer nanosheet solution to obtain a conductive hydrogel; the embryonic period umbilical cord tissue is wrapped with the conductive hydrogel, and is cured by ultraviolet light to obtain the decellularized tissue nerve graft scaffold with electric conductivity. Compared with the prior art, the nerve graft scaffold has good electric conductivity, mechanical properties and degradability.

The invention relates generally to the fields of biology and life sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in morselized amniotic membrane tissue and morselized umbilical cord tissue preparations.

The invention belongs to the field of biological medicine, and discloses a mesenchymal stem cell-derived exosome and active factor secretion composition and a directional preparation method thereof. According to the method, umbilical cord tissues of healthy mammals are used as raw materials, primary stem cells are separated through enzymolysis, and amplification and passage are conducted to 3-5 generations through an alpha-MEM culture medium; a serum-free induction culture medium containing multiple functional components is adopted for directional induction, a conditioned culture medium is collected, gradient centrifugation and ultrafiltration concentration are performed, and the protein concentration is calibrated to be 2 mg / mL, so that a finished product is obtained. The product can synergistically realize moisturizing, anti-wrinkle and tightening effects. Specifically, the exosome and active factor secreted composition is a composite system which is obtained by separating and concentrating mesenchymal stem cells from a conditioned medium of the mesenchymal stem cells after the mesenchymal stem cells are directionally induced by the method and is mainly composed of the exosome, growth factors self-secreted by the stem cells, cell factors and macromolecular active protein.

Provided are a novel composition for cartilage repair including a cell having a feature suitable for cartilage repair, and a production method therefor. The inventors of the present invention have paid attention to an umbilical cord tissue-derived cell, and have performed the three-dimensional culture of the umbilical cord tissue-derived cell. The inventors have found that the umbilical cord tissue-derived cell for forming a cultured product obtained by the three-dimensional culture has the following features: its cell proliferation potency and gene expression level are different from those of a cell for forming a cultured product obtained by the three-dimensional culture of cells derived from any other tissue. The umbilical cord tissue-derived cell of the present invention for forming the cultured product obtained by the three-dimensional culture has a feature suitable for cartilage repair, and hence can provide a composition effective in the cartilage repair.

The automatic device comprises a control box, a heat preservation cylinder, a digestion container and a magnetic transmission mechanism, the magnetic transmission mechanism comprises a stirrer and a rotary magnetic force generating part, the stirrer is placed in the digestion container, the rotary magnetic force generating part is installed on the control box, and the heat preservation cylinder is installed on the control box. The stirrer is specifically composed of a cross-shaped plastic shell and a first permanent magnet, the cross-shaped plastic shell is arranged on the periphery of the first permanent magnet in a wrapping mode, and inclined faces are arranged at the lower ends of the four corners of the cross-shaped plastic shell. According to the automatic device for rapidly digesting the umbilical cord tissue, the rotating speed of the output shaft of the motor is controlled through a controller in the control box, a second permanent magnet and a plurality of third permanent magnets can be driven to rotate at different speeds, a stirrer can be driven to perform rising, suspending and descending rotary stirring in liquid in a digestion container, the stirring range is larger, and the digestion efficiency is improved. The stirring effect is better.

Various cells, stem cells, and stem cell components, including associated methods of generating and using such cells are provided. In one aspect, for example, an isolated cell that is capable of self-renewal and culture expansion and is obtained from a subepithelial layer of a mammalian umbilical cord tissue. Such an isolated cell expresses at least three cell markers selected from CD29, CD73, CD90, CD166, SSEA4, CD9, CD44, CD146, or CD105, and does not express at least three cell markers selected from CD45, CD34, CD14, CD79, CD106, CD86, CD80, CD19, CD117, Stro-1, or HLA-DR.

The invention belongs to the technical field of stem cells, and particularly relates to a method for separating and culturing human umbilical cord mesenchymal stem cells. According to the method, the mesenchymal stem cells are separated from the human umbilical cord by adopting the low-concentration type II collagenase working solution, so that the digestion time is shortened, the production cost is reduced, and the cell damage is reduced. After treatment with type II collagenase, umbilical cord tissue blocks become loose, the tissue blocks are pre-cultured, and the mesenchymal stem cells are proliferated and enriched in the tissue blocks. The mesenchymal stem cells are separated from loose tissue blocks through mechanical blowing and are subjected to adherent culture, the culture time is short, the cell obtaining amount far exceeds that of a traditional adherent method, and the cell quality meets the standard requirement.

The utility model relates to the technical field of stem cell collection and extraction devices, in particular to an umbilical cord mesenchymal stem cell collection and extraction device which is characterized in that a stirring mechanism is started to stir crushed umbilical cord tissues and a stem cell decomposition solution, so that stem cells in the umbilical cord tissues are fully decomposed, and the umbilical cord mesenchymal stem cells are collected and extracted. The stem cell solution is discharged into the separating mechanism through the discharging mechanism, stem cells in the stem cell solution are separated by starting the separating mechanism, the solution is discharged through the discharging mechanism, and the practicability of the equipment is improved; comprising a feeding mechanism; the device further comprises a crushing mechanism, a stirring mechanism, a separating mechanism, a discharging mechanism and a cleaning mechanism, the crushing mechanism and the stirring mechanism are both installed in the feeding mechanism, the stirring mechanism is located below the crushing mechanism, the discharging mechanism is located on the right side of the feeding mechanism and installed in the separating mechanism, and the cleaning mechanism is installed on the left side of the feeding mechanism.

The application discloses a preparation method of umbilical cord decellularized matrix based on perfusion decellularization technology, which comprises decellularization treatment of a complete umbilical cord by using a programmed perfusion method, and cleaning, enzymatic hydrolysis, freeze-drying and other processes. The application further discloses the umbilical cord decellularized matrix prepared by the method and application thereof. The obtained umbilical cord decellularized matrix has complete structural integrity, retains not only umbilical cord Wharton's jelly but also rich low-immunogenic extracellular matrix components in umbilical cord tissues, and has wide application prospects in the development of soft tissue injury repair, medical beauty and life beauty related products.

The invention discloses a paraffin embedding demolding device for an umbilical cord tissue sample, and relates to the technical field of sample embedding, and the paraffin embedding demolding device comprises a base, a mounting piece, a bottom mold arranged on the mounting piece and an embedding box arranged on the bottom mold; a locking structure corresponding to the bottom die is arranged on the mounting piece; a cavity is formed in the bottom die, external rectangular columns are symmetrically arranged on the two long outer walls of the bottom die, circular channels communicated with the cavity are formed in the external rectangular columns, pistons are arranged in the circular channels, the upper ends of piston rods of the pistons extend out of the external rectangular columns, and supporting rods are fixedly arranged at the extending ends; external connecting plates are fixedly arranged on the outer edges of the upper parts of the two long outer walls of the embedding box, and when the embedding box is placed on the bottom die, the external connecting plates are lapped on the supporting rods; a hard air pipe capable of being communicated with the cavity is fixedly arranged on the base, and a sealing air channel communicated with the hard air pipe is arranged on the base. The paraffin embedding and demolding device for the umbilical cord tissue sample has the beneficial effect that the paraffin embedding and demolding device for the umbilical cord tissue sample is provided.

The invention discloses a perfusion decellularization technology-based preparation method of an umbilical cord decellularization matrix, which comprises the following steps: carrying out decellularization treatment on a complete umbilical cord by using a programmed perfusion method, cleaning, carrying out enzymolysis, freeze-drying and the like, and also discloses the umbilical cord decellularization matrix prepared by the method and application thereof. The obtained umbilical cord acellular matrix has structural integrity, not only retains umbilical cord Wharton jelly, but also retains rich low-immunogenicity extracellular matrix components in umbilical cord tissues, and has wide application prospects in development of soft tissue injury repair, medical cosmetology and life cosmetology related products.

The present invention provides a cartilage repair membrane and a preparation method thereof. The cartilage repair membrane includes an umbilical cord layer, a first gel layer, and a second gel layer that are stacked in sequence. The umbilical cord layer is a sheet-like structure composed of umbilical cord tissue, and the umbilical cord tissue is formed from an umbilical cord from which vascular cells have been removed by mechanical means. Both the first gel layer and the second gel layer are formed by mixing and cross-linking chitosan, sodium alginate, and hydroxyapatite, and the concentration of hydroxyapatite in the first gel layer and the second gel layer is different. The cartilage repair membrane of the present invention has good biological activity, mechanical properties, porosity, and osteogenic properties, provides the best repair effect for the treatment of cartilage defects, and has significant effects in aspects such as antibacterial, hemostatic, promoting the adhesion, proliferation of osteoblasts, and the formation of mineralized bone matrix, can meet the requirements of clinical and tissue engineering to the greatest extent, and can improve the repair degree of articular cartilage injury.

Various cells, stem cells, and stem cell components, including associated methods of generating and using such cells are provided. In one aspect, for example, an isolated cell that is capable of self-renewal and culture expansion and is obtained from a subepithelial layer of a mammalian umbilical cord tissue. Such an isolated cell expresses at least three cell markers selected from CD29, CD73, CD90, CD166, SSEA4, CD9, CD44, CD146, or CD105, and does not express at least three cell markers selected from CD45, CD34, CD14, CD79, CD106, CD86, CD80, CD19, CD117, Stro-1, or HLA-DR.

The present application belongs to the technical field of stem cells, and particularly relates to a method for separating and culturing human umbilical cord mesenchymal stem cells. The present application uses a low-concentration type II collagenase working solution to separate mesenchymal stem cells from a human umbilical cord, shortens the digestion time, reduces the production cost, and reduces cell damage. After being treated by the type II collagenase, the umbilical cord tissue block becomes loose, the tissue block is pre-cultured, and the mesenchymal stem cells proliferate and enrich in the tissue block. The mesenchymal stem cells are separated from the loose tissue block by mechanical blowing and are subjected to adherent culture, the culture time is short, the obtained cell amount is far more than that of the traditional adherent method, and the cell quality meets the standard requirements.

The invention discloses a cryopreservation method of umbilical cord tissues, which comprises the following steps: (1) mixing the umbilical cord tissues and a cryopreservation solution, balancing under a low-temperature condition, and cooling to a biological sample storage temperature; the L-carnitine is contained in the cryopreservation liquid, and the L-carnitine accounts for 0.03 M to 0.5 M. L-carnitine, DMSO and human serum albumin are applied to umbilical cord tissue sample cryopreservation, and L-carnitine obtains an unexpected effect better than trehalose under the same concentration condition. The cell viability and the recovery rate after cryopreservation are obviously improved.

The invention belongs to the technical field of cell culture, and particularly relates to a separation method and application of umbilical cord mesenchymal stem cells of down producing goats. The method comprises the following steps: firstly, separating Wharton's jelly from umbilical cord tissues, and cutting the Wharton's jelly into pieces to obtain tissue blocks; then putting the obtained tissue blocks into collagenase digestive juice, performing centrifugal treatment after digestion is completed, and then adding UC-MSCs complete culture medium with the same volume for culture, so as to obtain the umbilical cord mesenchymal stem cells. The result of the embodiment shows that the umbilical cord mesenchymal stem cells separated by the collagenase digestion method provided by the invention are good in growth state, strong in cell activity and stable in physiological state; meanwhile, the method is high in repeatability and simple in operation step, and a large number of primary seed cells can be obtained in a short time.

The invention discloses a preparation method of cat umbilical cord mesenchymal stem cells, and belongs to the technical field of cat stem cells. According to the improved preparation method of the cat umbilical cord mesenchymal stem cells, a pancreatin pretreatment mode is utilized, so that the umbilical cord tissue is quickly adhered to the wall, tissue loss caused by slow adhesion in the liquid changing process is reduced, the utilization rate of the umbilical cord is increased, and meanwhile, the preparation amount of the mesenchymal stem cells is increased. In addition, the umbilical cord mesenchymal stem cells prepared by the method have good differentiation potential and also have a positive treatment effect on cats with oral inflammation.

The present invention relates to a method for isolating umbilical cord-derived stem cells, and more specifically to a method for isolating a significantly large number of mesenchymal stem cells from the umbilical cord having a specified size. The method of the present invention has a great advantage in that since stem cells can be isolated from an umbilical cord tissue without enzymatic treatment, stress applied to the cells can be significantly suppressed. In addition, stem cells obtained by the method of the present invention have superior proliferative capacity compared to stem cells obtained by conventional isolation methods.

Provided is an isolated culture method for primary umbilical cord mesenchymal stem cells. The method comprises the following steps: S1, pretreating umbilical cord tissue; S2, adding the pretreated umbilical cord tissue to a digestive fluid containing type II collagenase, and performing digestion via shaking to obtain a digested mixed solution; S3, diluting the digested mixed solution with normal saline, performing centrifugation to collect crude precipitates, re-suspending same with normal saline, and performing centrifugation and washing to obtain precipitates; S4, transferring the precipitates into a culture solution, and performing suspension culture in a carbon dioxide incubator; and S5, transferring cells which have been subjected to the suspension culture to a sterile centrifuge tube, pipetting and mixing uniformly, performing centrifugation and washing to collect cell precipitates, and performing adherent culture on the cell precipitates to obtain primary umbilical cord mesenchymal stem cells. By means of the method, highly viable and highly proliferative primary UCMSCs can be obtained within 4–8 days from a serum-free and serum-substitute-free culture solution with clear components, thereby establishing a foundation for downstream development of new drugs and clinical research.