31 results about "Umbilical cord tissue" patented technology

The invention discloses a sleep-promoting nasal spray based on mesenchymal stem cell-derived exosomes and preparation and application thereof.The preparation method comprises the steps that 1, mesenchymal stem cells separated from umbilical cord tissue are cultured to P3-P5 generations, cell culture supernate is collected, cells are digested, and then cell debris and macromolecular impure protein are removed through pretreatment; (2) filtering and concentrating the pretreated cell culture supernatant and replacing a part of solution; (3) carrying out degerming filtration on the solution obtained in the step (2) through a 0.22 [mu] m filter membrane to obtain an MSC-Exos solution; and (4) adding a stabilizer with a proper concentration into the MSC-Exos solution to obtain a compound MSC-Exos solution, namely the sleep-promoting nasal spray. The product is a cell-derived natural active substance, can significantly improve sleep, and does not involve any pharmaceutical ingredient.

The invention discloses a skin-tightening anti-wrinkle essence and a preparation process thereof, and relates to the technical field of cosmetic preparation, and the preparation process comprises the following steps: obtaining animal umbilical cord tissue, culturing, collecting cell culture supernatant after culture, carrying out low-speed centrifugation to remove cells and dead cells, and carrying out concentration, filtration and sterilization through a tangential flow membrane technology to obtain the skin-tightening anti-wrinkle essence. The animal umbilical cord extract is obtained; the preparation method comprises the following steps: mixing the animal umbilical cord extract with water, butanediol, glycerol, hydroxypropyl tetrahydropyrantriol, 1, 2-hexanediol, trehalose, p-hydroxyacetophenone, allantoin, hydrolyzed sodium hyaluronate, sodium hyaluronate, tetrahydromethylpyrimidine carboxylic acid, xanthan gum and essence, homogenizing under a stirring condition, and standing for defoaming. By constructing an active stable microenvironment and an efficient transdermal delivery system, the technical problem that the animal umbilical cord extract serving as a core component is easy to inactivate and difficult to absorb is solved, and a faster and more stable skin structure remodeling effect is realized by utilizing the synergistic effect of the animal umbilical cord extract and functional components.

The present disclosure provides methods of treating Autism Spectrum Disorder. More particularly, the present disclosure relates to methods of using cord blood tissue-derived mesenchymal stromal cells (hCT-MSCs) to treat Autism Spectrum Disorder. The methods involve treating a patient having or suspected of having an Autism Spectrum Disorder by administering a therapeutically effective amount of hCT-MSCs.

The invention discloses hydrogel based on umbilical cord tissue and chorion tissue, which is prepared by a method comprising the following steps: (1) taking a placenta, shearing the umbilical cord and separating the chorion; (2) respectively carrying out tissue crushing on the umbilical cord and the chorion, and centrifugally collecting precipitates; mixing, washing, centrifuging and collecting precipitates; (3) freezing with liquid nitrogen, and then melting; repeatedly freezing and thawing; adding a buffer solution containing triton-X-100, and carrying out soaking treatment; rinsing, centrifuging and collecting precipitates; (4) adding urea, oscillating, centrifuging and collecting precipitate; freezing and drying to obtain freeze-dried powder; (5) dissolving the freeze-dried powder in hydrochloric acid, adding pepsin, and reacting; and adjusting the pH value to 7-7.5 to obtain the hydrogel. The hydrogel is applied to culture of cells, tissues or organoids, and is applied to preparation of a culture medium with the effect of promoting angiogenesis. The method is of great significance to development in the fields of regenerative medicine, disease modeling, medicine development and the like.

The invention relates generally to the fields of biology and health sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in amniotic membrane tissue and umbilical cord tissue preparations.

The invention relates generally to the fields of biology and health sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in amniotic membrane tissue and umbilical cord tissue preparations.

The invention provides an umbilical cord tissue cryopreservation solution, a cryopreservation method and a method for extracting umbilical cord mesenchymal stem cells. The freezing medium for the umbilical cord tissue comprises a basic permeable protective agent with the concentration of 5% v / v to 20% v / v, a non-permeable protective agent with the concentration of 5% v / v to 30% v / v, an antioxidant with the concentration of 0.1% v / v to 2% v / v, an ice control molecular material with the concentration of 0.001% v / v to 5% v / v, a buffer system with the concentration of 10 mM to 100 mM, an energy substance or antioxidant with the concentration of 0.1 mM to 10 mM, an ion carrier with the concentration of 0.1 mM to 5 mM and a membrane stabilizer. The cryopreservation solution for the umbilical cord tissue can ensure that cells in the umbilical cord tissue keep a survival state at low temperature, the preservation time of the cryopreservation solution is prolonged to several months and even one year, the preservation time of the umbilical cord is effectively prolonged, and the cryopreservation solution is far superior to an existing cryopreservation scheme.

The invention belongs to the technical field of cell culture, and particularly relates to a construction method of an immortalized goat umbilical cord mesenchymal stem cell line. The method comprises the following steps: by taking newborn goat lamb umbilical cord tissues as a research object, cleaning goat lamb umbilical cords, stripping blood vessels, separating Wharton's jelly, cutting the Wharton's jelly into 1mm < 3 > tissues, culturing by using a DMEM / F12 complete culture medium containing fetal calf serum, carrying out passage, cryopreservation, repeated resuspension and centrifugation, and inoculating into a culture dish for culture; lentiviruses containing SV40 large T antigens are packaged in vitro and are transduced into goat umbilical cord mesenchymal stem cells, and immortalization is realized after long-term subculture. The result of the embodiment shows that the construction method of the immortalized goat umbilical cord mesenchymal stem cell line provided by the invention is strong in repeatability, simple and efficient in operation steps, good in stem cell growth state and stable in physiological state.

The application discloses a preparation method of a decellularized tissue nerve graft scaffold with electric conductivity, and belongs to the field of biomaterials. The method comprises the following steps: separating embryonic period umbilical cord tissue, removing cells and lipids, and then performing freeze-drying; a titanium carbide monolayer nanosheet solution is prepared by using a chemical etching method, a GelMA solution is prepared by using a photo initiator phosphate buffer salt solution, and the GelMA solution is mixed with the titanium carbide monolayer nanosheet solution to obtain a conductive hydrogel; the embryonic period umbilical cord tissue is wrapped with the conductive hydrogel, and is cured by ultraviolet light to obtain the decellularized tissue nerve graft scaffold with electric conductivity. Compared with the prior art, the nerve graft scaffold has good electric conductivity, mechanical properties and degradability.

The invention relates generally to the fields of biology and life sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in morselized amniotic membrane tissue and morselized umbilical cord tissue preparations.

The invention belongs to the field of biological medicine, and discloses a mesenchymal stem cell-derived exosome and active factor secretion composition and a directional preparation method thereof. According to the method, umbilical cord tissues of healthy mammals are used as raw materials, primary stem cells are separated through enzymolysis, and amplification and passage are conducted to 3-5 generations through an alpha-MEM culture medium; a serum-free induction culture medium containing multiple functional components is adopted for directional induction, a conditioned culture medium is collected, gradient centrifugation and ultrafiltration concentration are performed, and the protein concentration is calibrated to be 2 mg / mL, so that a finished product is obtained. The product can synergistically realize moisturizing, anti-wrinkle and tightening effects. Specifically, the exosome and active factor secreted composition is a composite system which is obtained by separating and concentrating mesenchymal stem cells from a conditioned medium of the mesenchymal stem cells after the mesenchymal stem cells are directionally induced by the method and is mainly composed of the exosome, growth factors self-secreted by the stem cells, cell factors and macromolecular active protein.

Provided are a novel composition for cartilage repair including a cell having a feature suitable for cartilage repair, and a production method therefor. The inventors of the present invention have paid attention to an umbilical cord tissue-derived cell, and have performed the three-dimensional culture of the umbilical cord tissue-derived cell. The inventors have found that the umbilical cord tissue-derived cell for forming a cultured product obtained by the three-dimensional culture has the following features: its cell proliferation potency and gene expression level are different from those of a cell for forming a cultured product obtained by the three-dimensional culture of cells derived from any other tissue. The umbilical cord tissue-derived cell of the present invention for forming the cultured product obtained by the three-dimensional culture has a feature suitable for cartilage repair, and hence can provide a composition effective in the cartilage repair.

Various cells, stem cells, and stem cell components, including associated methods of generating and using such cells are provided. In one aspect, for example, an isolated cell that is capable of self-renewal and culture expansion and is obtained from a subepithelial layer of a mammalian umbilical cord tissue. Such an isolated cell expresses at least three cell markers selected from CD29, CD73, CD90, CD166, SSEA4, CD9, CD44, CD146, or CD105, and does not express at least three cell markers selected from CD45, CD34, CD14, CD79, CD106, CD86, CD80, CD19, CD117, Stro-1, or HLA-DR.

The utility model relates to the technical field of stem cell collection and extraction devices, in particular to an umbilical cord mesenchymal stem cell collection and extraction device which is characterized in that a stirring mechanism is started to stir crushed umbilical cord tissues and a stem cell decomposition solution, so that stem cells in the umbilical cord tissues are fully decomposed, and the umbilical cord mesenchymal stem cells are collected and extracted. The stem cell solution is discharged into the separating mechanism through the discharging mechanism, stem cells in the stem cell solution are separated by starting the separating mechanism, the solution is discharged through the discharging mechanism, and the practicability of the equipment is improved; comprising a feeding mechanism; the device further comprises a crushing mechanism, a stirring mechanism, a separating mechanism, a discharging mechanism and a cleaning mechanism, the crushing mechanism and the stirring mechanism are both installed in the feeding mechanism, the stirring mechanism is located below the crushing mechanism, the discharging mechanism is located on the right side of the feeding mechanism and installed in the separating mechanism, and the cleaning mechanism is installed on the left side of the feeding mechanism.

The application discloses a preparation method of umbilical cord decellularized matrix based on perfusion decellularization technology, which comprises decellularization treatment of a complete umbilical cord by using a programmed perfusion method, and cleaning, enzymatic hydrolysis, freeze-drying and other processes. The application further discloses the umbilical cord decellularized matrix prepared by the method and application thereof. The obtained umbilical cord decellularized matrix has complete structural integrity, retains not only umbilical cord Wharton's jelly but also rich low-immunogenic extracellular matrix components in umbilical cord tissues, and has wide application prospects in the development of soft tissue injury repair, medical beauty and life beauty related products.

The invention discloses a paraffin embedding demolding device for an umbilical cord tissue sample, and relates to the technical field of sample embedding, and the paraffin embedding demolding device comprises a base, a mounting piece, a bottom mold arranged on the mounting piece and an embedding box arranged on the bottom mold; a locking structure corresponding to the bottom die is arranged on the mounting piece; a cavity is formed in the bottom die, external rectangular columns are symmetrically arranged on the two long outer walls of the bottom die, circular channels communicated with the cavity are formed in the external rectangular columns, pistons are arranged in the circular channels, the upper ends of piston rods of the pistons extend out of the external rectangular columns, and supporting rods are fixedly arranged at the extending ends; external connecting plates are fixedly arranged on the outer edges of the upper parts of the two long outer walls of the embedding box, and when the embedding box is placed on the bottom die, the external connecting plates are lapped on the supporting rods; a hard air pipe capable of being communicated with the cavity is fixedly arranged on the base, and a sealing air channel communicated with the hard air pipe is arranged on the base. The paraffin embedding and demolding device for the umbilical cord tissue sample has the beneficial effect that the paraffin embedding and demolding device for the umbilical cord tissue sample is provided.

Various cells, stem cells, and stem cell components, including associated methods of generating and using such cells are provided. In one aspect, for example, an isolated cell that is capable of self-renewal and culture expansion and is obtained from a subepithelial layer of a mammalian umbilical cord tissue. Such an isolated cell expresses at least three cell markers selected from CD29, CD73, CD90, CD166, SSEA4, CD9, CD44, CD146, or CD105, and does not express at least three cell markers selected from CD45, CD34, CD14, CD79, CD106, CD86, CD80, CD19, CD117, Stro-1, or HLA-DR.

The present application belongs to the technical field of stem cells, and particularly relates to a method for separating and culturing human umbilical cord mesenchymal stem cells. The present application uses a low-concentration type II collagenase working solution to separate mesenchymal stem cells from a human umbilical cord, shortens the digestion time, reduces the production cost, and reduces cell damage. After being treated by the type II collagenase, the umbilical cord tissue block becomes loose, the tissue block is pre-cultured, and the mesenchymal stem cells proliferate and enrich in the tissue block. The mesenchymal stem cells are separated from the loose tissue block by mechanical blowing and are subjected to adherent culture, the culture time is short, the obtained cell amount is far more than that of the traditional adherent method, and the cell quality meets the standard requirements.

The invention discloses a cryopreservation method of umbilical cord tissues, which comprises the following steps: (1) mixing the umbilical cord tissues and a cryopreservation solution, balancing under a low-temperature condition, and cooling to a biological sample storage temperature; the L-carnitine is contained in the cryopreservation liquid, and the L-carnitine accounts for 0.03 M to 0.5 M. L-carnitine, DMSO and human serum albumin are applied to umbilical cord tissue sample cryopreservation, and L-carnitine obtains an unexpected effect better than trehalose under the same concentration condition. The cell viability and the recovery rate after cryopreservation are obviously improved.

The invention belongs to the technical field of cell culture, and particularly relates to a separation method and application of umbilical cord mesenchymal stem cells of down producing goats. The method comprises the following steps: firstly, separating Wharton's jelly from umbilical cord tissues, and cutting the Wharton's jelly into pieces to obtain tissue blocks; then putting the obtained tissue blocks into collagenase digestive juice, performing centrifugal treatment after digestion is completed, and then adding UC-MSCs complete culture medium with the same volume for culture, so as to obtain the umbilical cord mesenchymal stem cells. The result of the embodiment shows that the umbilical cord mesenchymal stem cells separated by the collagenase digestion method provided by the invention are good in growth state, strong in cell activity and stable in physiological state; meanwhile, the method is high in repeatability and simple in operation step, and a large number of primary seed cells can be obtained in a short time.

The invention discloses a preparation method of cat umbilical cord mesenchymal stem cells, and belongs to the technical field of cat stem cells. According to the improved preparation method of the cat umbilical cord mesenchymal stem cells, a pancreatin pretreatment mode is utilized, so that the umbilical cord tissue is quickly adhered to the wall, tissue loss caused by slow adhesion in the liquid changing process is reduced, the utilization rate of the umbilical cord is increased, and meanwhile, the preparation amount of the mesenchymal stem cells is increased. In addition, the umbilical cord mesenchymal stem cells prepared by the method have good differentiation potential and also have a positive treatment effect on cats with oral inflammation.

Provided is an isolated culture method for primary umbilical cord mesenchymal stem cells. The method comprises the following steps: S1, pretreating umbilical cord tissue; S2, adding the pretreated umbilical cord tissue to a digestive fluid containing type II collagenase, and performing digestion via shaking to obtain a digested mixed solution; S3, diluting the digested mixed solution with normal saline, performing centrifugation to collect crude precipitates, re-suspending same with normal saline, and performing centrifugation and washing to obtain precipitates; S4, transferring the precipitates into a culture solution, and performing suspension culture in a carbon dioxide incubator; and S5, transferring cells which have been subjected to the suspension culture to a sterile centrifuge tube, pipetting and mixing uniformly, performing centrifugation and washing to collect cell precipitates, and performing adherent culture on the cell precipitates to obtain primary umbilical cord mesenchymal stem cells. By means of the method, highly viable and highly proliferative primary UCMSCs can be obtained within 4–8 days from a serum-free and serum-substitute-free culture solution with clear components, thereby establishing a foundation for downstream development of new drugs and clinical research.

This invention discloses a method and application for rapidly preparing humanized collagen using umbilical cord mesenchymal stem cell material, belonging to the field of biomaterial preparation technology. The key technical points of the method are as follows: S1, processing and passage-expanding umbilical cord tissue; S2, seeding the expanded umbilical cord mesenchymal stem cell suspension into a culture dish, then adding DMEM / F12 medium containing platelet lysis buffer for culture, followed by induction medium to obtain extracellular matrix; S3, enzymatically hydrolyzing the extracellular matrix, centrifuging the hydrolysate to obtain the supernatant, adjusting the pH, allowing it to stand, centrifuging again to obtain the precipitate, reconstituteing the precipitate, purifying it, centrifuging again, and then lyophilizing it; S4, forming the formulation to achieve efficient secretion and self-assembly of collagen, while utilizing extracellular matrix components (such as fibronectin and laminin) secreted by umbilical cord mesenchymal stem cells to assist collagen in forming an active product with a natural triple helix structure.

The invention discloses a mesenchymal stem cell for treating type 2 diabetes mellitus, a preparation method and application. Belongs to the technical field of biology. The method comprises the following steps: firstly, separating mesenchymal stem cells from umbilical cord tissues, and then efficiently transducing a lentiviral vector carrying a Glymaxylin gene into the cells by adopting an optimized centrifugal enhancement method. The transduction process is carried out under a specific transduction enhancer, the infection complex number and the cell fusion degree are accurately controlled, and then centrifugation is carried out to greatly improve the transduction efficiency. And screening the transduced cells, and amplifying the cells in a serum-free culture medium added with bFGF (basic fibroblast growth factor) and / or EGF (epidermal growth factor), so as to finally obtain the high-activity and high-expression Glymaxylin gene mesenchymal stem cell for treatment. By systematically optimizing the preparation process, the problem of unstable transduction efficiency is solved, and the prepared cell product can effectively promote the functional recovery of the pancreatic beta cells and lays a solid foundation for developing cell drugs.

The utility model relates to the technical field of cutters, and discloses an umbilical cord tissue cutter which comprises a fixing frame and an outer cylinder, an installation plate is fixedly installed on the inner side of the fixing frame, a fixing cylinder is fixedly installed at the bottom of the installation plate, an installation block is fixedly installed outside the fixing cylinder, an installation mechanism is arranged in the outer cylinder, and the installation block is fixedly installed on the outer cylinder. A cutting mechanism is arranged in the fixed cylinder, the outer cylinder and the fixed cylinder are mounted through a threaded cylinder, a spring drives a circular ring to move upwards, so that an inserting column is inserted into the outer cylinder, and the situation that when umbilical cord tissue is cut, axial force drives a filter cylinder to rotate, so that connection between the threaded cylinder and the fixed cylinder is loosened due to rotation of the outer cylinder is avoided; according to the umbilical cord cutting device, the outer cylinder is installed more firmly and is more stable during use, the inserting block is inserted into the rotating shaft, the cutting knife rotates to cut the umbilical cord tissue, cutting of the umbilical cord tissue is faster and more convenient, and meanwhile pollution is avoided through sealed cutting.

The invention provides a method for separating human umbilical cord mesenchymal stem cells. The umbilical cord mesenchymal stem cells are obtained by sequentially carrying out umbilical cord tissue treatment, umbilical cord sheet treatment and umbilical cord block culture and separation. The method disclosed by the invention is simple to operate, the separation efficiency of the umbilical cord tissue is improved, the purity of the obtained cells is higher and is obviously superior to the requirement of the ISCT lowest identification standard, the production cost can be effectively reduced, and industrial application is facilitated.

The present invention aims to provide a novel agent for the prevention and / or treatment of organ fibrosis. [Solution] The present invention is a preventive and / or therapeutic agent for organ fibrosis, comprising mesenchymal stem cell culture supernatant. The mesenchymal stem cell culture supernatant is preferably a culture supernatant obtained from the culture of mesenchymal stem cells derived from adipose tissue, umbilical cord tissue, or bone marrow tissue. Furthermore, the organ is preferably the kidney.

The invention relates to the technical field of medicine research and development, in particular to a preparation method of stem cell exosomes and application of the stem cell exosomes in skin aging deficit, and the preparation method comprises the following steps: S1, taking clean umbilical cord tissues, and placing the clean umbilical cord tissues in a culture medium; the method comprises the following steps: uniformly spreading sheared tissue blocks in culture dishes, adding a culture medium into each dish, continuously filling oxygen into the culture medium in the culture process, collecting cell culture supernate, pretreating a sample to remove cell debris and large-particle substances, centrifuging cells, and storing at-80 DEG C, so as to obtain the cell culture medium. 1 mg of exosome is extracted and fully mixed with 10 ml of normal saline, the stem cell exosome injection is prepared for standby application, under the condition that the skin structure is not damaged, the stem cell exosome is injected into the deep layer of the skin through a microneedle, the absorption rate of the exosome is increased, the anti-aging effect is enhanced, the stem cell exosome can promote skin proliferation, and the anti-aging effect is improved. The stem cell exosome can regulate and control cell migration, and the stem cell exosome can influence cell apoptosis.

The utility model discloses a special scalpel for umbilical cord tissue culture, and relates to the technical field of scalpels. The cutter comprises a cutter handle and a blade, the blade is inserted and clamped on the connecting section; the blade comprises an inserting section and a tool bit; an included angle of 135 degrees is formed between the tool bit and the inserting section; a blade inserting hole correspondingly matched with the clamping seam is formed in the surface of the inserting section, a limiting protrusion correspondingly matched with the blade inserting hole is arranged on the inner bottom face of the clamping seam, and a distance is formed between the limiting protrusion and the inner top face of the clamping seam. The cutter head and the cutter handle are tightly connected together through the embedded structure of the cutter head and the cutter handle, the cutter head is fixed and cannot rotate, and when the cutter handle is held by a hand, the included angle between the cutter handle and the blade is 45 degrees, so that the cutter head can be parallel to a tissue block, needed tissue can be evenly stripped, the tissue is prevented from being torn, and the edge is not complete; the structure is simple and practical, the operation is convenient, the experiment progress is not influenced, and the operation convenience of the human umbilical cord tissue separation experiment is improved.