71 results about "Conditioned medium" patented technology

Provided herein are methods and related compositions for enhancing or enhanced partial reprogramming of target cells in a subject in need thereof (e.g., a human subject suffering from or at risk of a disease), the method comprising administering a plurality of mesenchymal lineage progenitor or stem cells (MLPSCs), exosomes derived therefrom, or conditioned culture media derived therefrom to a subject that expresses or will express one or more reprogramming factors in a population of target cells, whereby a plurality of the target cells in the subject become partially reprogrammed, but not fully reprogrammed.

The invention provides an aerosol-type face pack cosmetic that, when dispensed, forms a flexible, easy-to-use, and low-irritation resin foam that easily adheres to the face, such as around the eyes and nose. It also allows the fermented liquid contained in the resin foam to easily seep out, making it easy to apply the fermented liquid to the face and less likely to cause tightness. [Solution] An aerosol pack cosmetic comprising a beauty serum and dimethyl ether, wherein the beauty serum comprises at least one fermentation solution selected from Saccharomyces / Anzange ferment filtrate, Saccharomyces / Human (fibroblast / mononuclear cell) induced pluripotent cell culture conditioned medium ferment filtrate, Galactomyces / Eryngium multiflorum callus culture ferment filtrate, and a resin.

The invention relates to the technical field of biology, and discloses a construction method of a gastric cancer organoid culture system, which comprises the following steps: preparation of a conditioned medium: culturing L-WRN cells and collecting the conditioned medium; preparing a gastric cancer organoid culture medium, and mixing the conditioned culture medium, the basic culture medium, a plurality of growth factors and additives; and extraction and culture of organoid: separating cells from gastric cancer tissues, mixing the cells with matrigel, and culturing by using the prepared culture medium. According to the method, the repeatability and the stability of the organoid model are improved, the response consistency of the organoid in drug screening is improved, and the reliability of the model in disease mechanism research and personalized medical application is guaranteed.

The invention belongs to the technical field of biological medicines, and particularly relates to application of miR-206-3p in regulation and control of skeletal muscle atrophy, and the change condition of the miR-206-3p level is measured by constructing an in-vitro cancer cachexia myotubular atrophy model; it is verified that in-vitro addition of miR-206-3p mimics relieves in-vitro myotube atrophy induced by a cancer conditioned culture medium, inhibits expression of a myotube marker gene and inhibits proteolysis and metabolism pathways and cell apoptosis of myotubes, so that myotube atrophy induced by cancer cachexia is inhibited. The invention opens up a new thought for the research of cachexia pathogenesis, and also provides a potential new target for the research and development of cachexia treatment and / or cachexia prevention drugs.

The invention relates to the technical field of biological medicine, and provides application of a dental pulp stem cell exosome in preparation of a medicine for treating and preventing osteoarthritis, a preparation method of the dental pulp stem cell exosome comprises the following steps: S1, obtaining dental pulp tissue from a dental pulp cavity of an in-vitro tooth; s2, pretreating dental pulp tissues, adding digestive juice, digesting, centrifuging, discarding supernatant, adding a complete culture medium containing antibiotics into precipitates, mixing, filtering and centrifuging to obtain dental pulp stem cells; and S3, culturing the dental pulp stem cells by using a complete culture medium, when the confluence degree of the dental pulp stem cells reaches 60-80%, replacing the serum-free culture medium for continuous culture, collecting the dental pulp stem cell conditioned culture medium, and performing differential centrifugation to obtain the dental pulp stem cell exosome. According to the technical scheme, the regulating effect of the dental pulp stem cell exosome on endoplasmic reticulum stress and the mechanism of the dental pulp stem cell exosome for improving cartilage cell degeneration are explored.

The application discloses immortalized chicken embryo epithelioid cells and a preparation method and application thereof, and the preparation method of the immortalized chicken embryo epithelioid cells comprises the following steps: extracting primary chicken embryo epithelial cells from a chicken embryo; and culturing the primary chicken embryo epithelioid cells in a conditional medium containing chicken Noggin protein for subculture, so as to obtain the immortalized chicken embryo epithelioid cells. The immortalized chicken embryo epithelioid cells prepared by the preparation method can be stably subcultured for a long time, can maintain stable proliferation ability after multiple subcultures, reduces individual differences, and improves the reliability and repeatability of experimental results. In addition, the preparation method does not introduce exogenous genes or change the genotype of the cells, ensures the stability at the gene level, and avoids the interference of the exogenous genes on the stability of the cell phenotype.

The subject invention pertains to compositions of a probe comprising two pH-reversible forms, including the boron dipyrromethene (BODIPY) fluorescent form and the non-fluorescent leuco-BODIPY form, for use in confocal imaging methods of intracellular vesicles as well as extracellular vesicles that are present in conditioned culture medium and / or secreted by living cells, without inducing severe cytotoxicity.

Tissue-specific progenitor cells representing the endoderm, ectoderm, and mesoderm lineages are generated from pluripotent sources. Progenitor cell generation is accomplished by contacting pluripotent stem cells with tissue-specific exosomes, microRNAs, proteins, and peptides, obtained by stressing the tissue under in vitro conditions. In vitro-generated tissue organoids are used as a source of "differentiation factors" for generating individualized progenitor cells. For example, to generate lung progenitor cells, allogeneic stem cells are implanted into decellularized cadaveric lung tissue to form lung structures, which are then exposed to various cellular stressors and conditioned media to differentiate the pluripotent stem cells into progenitor cells. Extracellular matrix can be obtained from decellularized structures seeded with regenerating cells and used to generate organoids, which are then subjected to stress to produce differentiation factors.

The invention belongs to the technical field of tissue engineering and aging resistance, and particularly relates to a composition for delaying cell aging and maintaining tissue homeostasis as well as a preparation method and application of the composition. Specifically, research finds that adipose tissues, skin tissues and the like are placed in a serum-free basal culture medium, adherent culture is performed, the obtained supernate is collected, and the obtained serum-free conditioned culture medium preparation can effectively inhibit aging of cells (such as ADSCs or HDFs) and maintain the functional steady state of the cells in in-vitro culture. Meanwhile, the culture medium product provided by the invention is also proved to be capable of inhibiting an IL-6 / STAT3 signal channel, and the channel is revealed to be a core mechanism of the anti-aging effect of the culture medium product, so that mechanism support is provided for the application of the culture medium product to cell aging resistance, and the culture medium product has good practical application value.

The utility model discloses a stem cell conditioned medium filter device which comprises a filter device shell, a filter tank is arranged in the filter device shell, a square sleeve is arranged on the lower side in the filter tank, a coarse-mesh filter screen is fixedly arranged in the square sleeve, and a filter screen is arranged in the coarse-mesh filter screen. A plurality of fine-hole filter screens fixed in the square sleeve are fixedly arranged below the coarse-hole filter screen; a sealing plate fixed in the square sleeve is arranged above the coarse-mesh filter screen, and a first stepping motor is fixedly mounted in the sealing plate. According to the utility model, the situation that the filter holes in the filter screen are blocked by cell debris, dead cells or metabolic wastes in the stem cell conditioned culture medium and even penetrate through the filter holes due to relatively intense impact generated between the filter screen and the stem cell conditioned culture medium when the filter screen is used for filtering the stem cell conditioned culture medium is prevented; through the technical scheme, the filtering effect of the equipment is improved.

The present invention provides compositions and methods for treating cardiac medical conditions such as heart failure caused by damaged myocardial tissue, as well as compositions and methods for addressing inflammatory and fibrotic processes observed in a wide range of medical conditions. [Solution] Embodiments of the present disclosure relate to compositions and methods of use relating to specific immortalized cells, including cardiomyocytes, obtained from pediatric or neonatal individuals. In specific embodiments, immortalized cells, or cell-derived conditioning media, or a partial or whole secretome thereof, are provided to individuals requiring it in an effective amount, either alone or in combination with cardiomyocytes.

The invention relates to methods for extracting, purifying and concentrating mesenchymal stromal cell exosomes enriched with micro-RNA, proteins and lipids, and can be used in cosmetology and pharmacology for rehabilitation and regeneration. The technical result of the invention is a more efficient process for producing exosomes. This technical result is achieved in that the present method for extracting, purifying and concentrating exosomes from mesenchymal stromal cells includes cultivating cells, collecting the conditioned culture medium, depleting high-molecular-weight proteins, extracting, purifying and concentrating exosomes, and freezing and lyophilizing the exosome concentrate; in order to enrich the culture medium with exosomes, multipotent mesenchymal stem cells (MSCs) are grown in a medium having a lowered glucose concentration and containing platelet lysate and 5 mM of L-alanyl-L-glutamine, wherein, in the case of static cell cultivation, the growth medium is placed in a culture vessel and once a level of not less than 80% monolayer confluence is reached, the culture medium is harvested and replaced once every 24 hours, and in the case of flow-through cell cultivation, cells are placed on cell carriers in a bioreactor and 72-96 hours after the start of cultivation, the culture medium is harvested and replaced once every 24 hours, the exosome-enriched culture medium is subjected to concentration, which includes successive stages of centrifugation, tangential flow filtration and isopycnic centrifugation: in the first stage, the culture medium is centrifuged for 10 mins at 2000 g to remove large cell particles and other inclusions; in the tangential flow filtration stage, the supernatant is concentrated using hollow fibre filters having a molecular weight cutoff of 500 kDa, after which the concentrate is subjected to fivefold diafiltration with an equal volume of DPBS phosphate buffer solution using the same hollow fibre cartridge; and in the isopycnic centrifugation stage, the concentrated supernatant is mixed with a saccharose solution and deuterium oxide and subjected to isopycnic centrifugation at 100000 g and 4˚С for 75 mins, after which the concentrate is subjected to further fivefold diafiltration with a buffer solution, then the concentrate is filtered through filters with a pore diameter of 0.22 µm and 0.1 µm, the exosome concentrate is subjected to initial freezing to -75˚C in a freezing chamber, and drying is carried out in a lyophilization chamber, wherein in a first drying stage, the concentrate is cooled to -40˚С and subsequently held for 100 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -30˚С and subsequently held for 610 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -10˚С and subsequently held for 315 mins at an absolute pressure in the chamber of not more than 10 Pa, after which it is cooled to 0˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa, then heated to +10˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa; in a second drying stage, drying is carried out at +22˚С for not less than 130 minutes at an absolute pressure in the chamber of not more than 10 Pa.

The present invention relates to a method for inducing, stimulating, and / or promoting the healing of a wound or damaged skin area, comprising topically treating a subject's skin surrounding the wound or damaged skin area with conditioned medium derived from a culture of umbilical cord mesenchymal stem cells. Similarly, the present invention relates to a method for preventing the formation and / or recurrence of wounds or damaged skin areas in a subject at risk of developing wounds or damaged skin areas, comprising topically treating the subject's skin with conditioned medium derived from a culture of umbilical cord mesenchymal stem cells. Furthermore, the present invention relates to a method for treating skin damage caused by rosacea, psoriasis, eczema, dermatitis, topical steroid withdrawal syndrome, epidermolysis bullosa, or fragile skin, comprising topically treating a subject's skin surrounding the wound or damaged skin area to be treated with conditioned medium derived from a culture of umbilical cord mesenchymal stem cells. The present invention is also directed to the conditioned medium, uses thereof, compositions comprising it, methods for producing the conditioned medium, and uses of these compositions.

The present disclosure provides methods related to tissue regeneration using human mesenchymal stem cell (MSC) / CD34+ hematopoietic stem / progenitor cell (HSPC) co-cultured total conditioned media. In particular, the present disclosure provides novel methods for treating and / or preventing damage to, trauma to, and / or loss of tissue.

The invention belongs to the field of biological medicine, and discloses a mesenchymal stem cell-derived exosome and active factor secretion composition and a directional preparation method thereof. According to the method, umbilical cord tissues of healthy mammals are used as raw materials, primary stem cells are separated through enzymolysis, and amplification and passage are conducted to 3-5 generations through an alpha-MEM culture medium; a serum-free induction culture medium containing multiple functional components is adopted for directional induction, a conditioned culture medium is collected, gradient centrifugation and ultrafiltration concentration are performed, and the protein concentration is calibrated to be 2 mg / mL, so that a finished product is obtained. The product can synergistically realize moisturizing, anti-wrinkle and tightening effects. Specifically, the exosome and active factor secreted composition is a composite system which is obtained by separating and concentrating mesenchymal stem cells from a conditioned medium of the mesenchymal stem cells after the mesenchymal stem cells are directionally induced by the method and is mainly composed of the exosome, growth factors self-secreted by the stem cells, cell factors and macromolecular active protein.

The method of hair transplant using dermal papilla and dermal sheath obtained from hair follicle as disclosed comprises the steps of: providing initial keratinocytes to be used for creating condition medium; creating and collecting condition medium from said keratinocytes; collecting hair follicle cells containing dermal papilla and dermal sheath from patients; culturing said cells as obtained from patients using condition medium collected from the prior step; injecting cultured cells in the predetermined amount and the predetermined ratio of dermal papilla cells and dermal sheath cells in the predetermined amount area characterized in that the step of injecting and collecting condition medium from said keratinocytes comprises the step of applying a solution obtained from the mixture of defined keratinocyte-serum free medium (DK-SFM) and Mesenchymal stem cell culture medium (MSC) to said initial keratinocytes, removing defined keratinocyte-serum free medium (DK-SFM), and extracting a condition medium from the said mixture and wherein the step of culturing hair follicle cells as obtained from patients comprises the step of applying condition medium extracted and MSC in the ratio of 1:1.

The invention relates to the technical field of pharmaceutical compositions, in particular to an atomization composition and application thereof. An aerosolized composition comprising: A) an MSC-derived active ingredient selected from any one or a combination of (i) MSC exosomes / extracellular vesicles, (ii) an MSC conditioned culture solution concentrate, (iii) an MSC lysate, (iv) an MSC-derived soluble factor, (v) an immobilized / embedded protected MSC or fragment thereof; b) ergothioneine (EGT) or a pharmaceutically acceptable salt thereof; and C) a pharmaceutically acceptable carrier / buffer system making it suitable for aerosol inhalation administration. The exosome is combined with ergothioneine, and the effectiveness of sleep improvement is remarkably improved through mechanism synergy of nerve regulation and cell protection; the introduction of the atomization drug delivery technology breaks through the limitation of the traditional therapy from multiple dimensions of non-invasiveness, accurate delivery, individualized treatment, accessibility and the like, so that wider crowds (including special crowds) can safely, conveniently and efficiently receive treatment.

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and / or the plant may also be cultured together to form conditioned media and associated growth factors.

The present disclosure is generally directed to the use of biomaterial scaffolds engineered with SA-FasL for the transplantation of stem cell derived β-cells as a treatment for Type I diabetes. Early engraftment post-transplantation and subsequent maturation of these β-cells may be limited by the initial inflammatory response, which impacts the ability to sustain normoglycemia at long times. The survival and development of immature hPSC-derived β-cells transplanted on poly(lactide-co-glycolide) (PLG) microporous scaffolds into the peritoneal fat, a site being considered for clinical translation, was investigated. The scaffolds were modified with biotin for binding of a streptavidin-FasL (SAFasL) chimeric protein to modulate the local inflammatory microenvironment. The presence of FasL impacted infiltration of monocytes and neutrophils and altered their phenotypic response. Conditioned media generated from scaffolds explanted at day 4 did not impact hPSC-derived β-cell survival and maturation in vitro, which was not observed with unmodified scaffolds. Following transplantation, β-cell viability and differentiation were improved with SA-FasL modification. A sustained increase in insulin positive cell ratio was observed with SA-FasL modified relative to unmodified scaffolds. These results demonstrate that SA-FasL-modified scaffolds can mitigate initial inflammatory response and enhance β-cell engraftment and differentiation.

The invention relates to a bovine umbilical cord cell, a preparation method thereof, a conditioned medium and an antibacterial method thereof. The bovine umbilical cord cell is obtained through a specific enzyme digestion and culture system, is fusiform and has high activity; the conditioned medium is obtained by culturing bovine umbilical cord cells in MEM-alpha for 24 hours, centrifuging, taking supernate and filtering. The invention has the beneficial effects that the cell and the conditioned medium prepared from the cell show extremely strong bacteriostatic activity on staphylococcus aureus and klebsiella pneumoniae, and the bacteriostatic rate can reach 95% or above; the antibacterial effect can be partially reversed by exogenous iron; the invention provides important cell resources and material basis for developing novel, efficient and cell-free antibacterial biological products, and has wide application prospect in the field of anti-infection treatment.

The invention provides application of detection of enterococcus faecalis in liver cancer tissue in treatment of liver cancer. The invention finds that enterococcus faecalis is highly abundant in liver tumor tissues and is positively correlated with the pathogenesis of HCC (Hepatocellular Carcinoma). Enterococcus faecalis or an enterococcus faecalis conditioned medium promotes liver cancer cell proliferation, protein translation, cell migration and tumorigenesis. Animal experiments prove that colonization of enterococcus faecalis can promote in-vivo growth of liver cancer, and everolimus (an mTOR pathway inhibitor) administration can significantly inhibit the promotion effect. The colonization amount of the enterococcus faecalis can be used as a marker of a liver cancer clinical treatment strategy of colonization of the enterococcus faecalis.

The application belongs to the technical field of cell biology, and particularly relates to a feeder-free and serum-free bovine embryonic stem cell culture medium, a culture system and a culture method. The bovine embryonic stem cell culture medium comprises a conditioned medium and an additive. The additive is IWR-1 and activin A. The concentrations of IWR-1 and activin A in the conditioned medium are 2.5-5 muM and 20 ng / mL-25 ng / mL respectively. The culture system comprises the bovine embryonic stem cell culture medium, 0.1%-0.2% gelatin in mass concentration and a subculture medium. The 0.1%-0.2% gelatin in mass concentration is used to provide an attachment matrix for the bovine embryonic stem cell. The subculture medium is prepared by adding Y27632 to the bovine embryonic stem cell culture medium. The application reduces the cost of extracellular matrix, and the feeder-free bovine embryonic stem cell has high single cell subculture efficiency and is convenient for gene editing operation.

The invention discloses a deciduous tooth pulp mesenchymal stem cell population, a separation and extraction method and a proliferation potential identification method. According to the separation method, SHED is efficiently separated from dental pulp tissues of deciduous teeth through an optimized mixed enzyme digestion method (3 mg / mL of neutral protease and 4 mg / mL of II type collagenase), and the separation success rate reaches 76% or above. According to the identification method, a proliferation-function-aging three-in-one evaluation system is constructed, and the method comprises the following steps: quantitatively evaluating proliferation potential through a clone formation rate (greater than or equal to 10%), an S-phase cell proportion (greater than or equal to 20%) and a Ki-67 positive rate (greater than or equal to 60%); the migration and paracrine capabilities are evaluated through a scratch experiment (the healing rate in 24 hours is greater than or equal to 35%) and a conditioned medium function experiment; the aging trend is evaluated through SA-beta-gal staining (the positive rate is lt; 10%). The quantitative standard of the high-quality SHED is defined for the first time, and reliable technical support is provided for quality control and standardized application of the high-quality SHED in regenerative medicine.

The embodiment of the application discloses a preparation method of humanized extracellular matrix, and belongs to the technical field of biomedical materials and tissue engineering. The method comprises the following steps: preparing human decellularized dermal matrix; preparing a concentrated solution of a condition medium rich in human active factors; constructing RGD-PLGA / BMP-2 nanocomposites; loading the concentrated solution of the condition medium into the human decellularized dermal matrix through vacuum negative pressure perfusion, and then anchoring the RGD-PLGA / BMP-2 nanocomposites, and cross-linking them twice through genipin to obtain the humanized extracellular matrix. Through the four-element strategy of decellularized matrix-active factor-nano-sustained release-two-stage cross-linking, the unity of low immunogenicity, high biological activity and spatiotemporal controllable release function is realized, and the humanized extracellular matrix can be applied to the fields of tissue engineering scaffolds, wound repair and bone defect filling.

The present invention relates to a method of inducing, stimulating and / or promoting hair growth and / or hair regeneration, wherein the method comprises treating the hair of a subject with conditioned medium derived from cultivation of mesenchymal stem cells of the umbilical cord. The present invention also relates to a method of alleviating and / or reducing hair loss and / or hair thinning, wherein the method comprises treating the hair a subject with conditioned medium derived from cultivation of mesenchymal stem cells of the umbilical cord. The invention also relates to a method of producing a conditioned medium, the method comprising: a) cultivating mesenchymal stem cells of the umbilical cord in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum); b) removing the mesenchymal stem cells of the umbilical cord from the culture medium; wherein the conditioned medium is obtained by collecting the cell culture medium. The invention also relates to a conditioned medium obtained or obtainable by the producing method, a composition thereof and uses thereof.

The present disclosure relates to the field of medicine, in particular to a protein nanoparticle, a preparation method thereof, a pharmaceutical composition containing the same and uses. The protein nanoparticle is a nanoparticle component obtained by pretreating the supernatant of a stress-treated mesenchymal stem cell conditioned medium and then purifying by size exclusion chromatography, and the chromatogram obtained by size exclusion chromatography contains at least a peak with an apparent molecular weight of 56-70 kDa.

The application relates to an oral drug delivery system with a double-emulsion structure and application thereof in preparation of a medicine for treating premature ovarian insufficiency, and belongs to the technical field of biological medicines. The oral drug delivery system comprises an oil-phase solvent, MSCs conditioned medium is wrapped in the inside to form a droplet particle, and exosomes are uniformly distributed on the outer surface of the droplet particle, thereby forming a double-emulsion structure. The water-soluble MSC-CM is wrapped by a water-in-oil-in-water method to form a double-emulsion structure, so that the water-soluble protein is absorbed into the human body in an oral mode. The application has good structural stability and viscoelasticity, the particle size range is about 10-50 mu m, the contact with the intestinal epithelial cells is facilitated, meanwhile, the skeleton of the intestinal epithelial cells can be changed, the expression of the tight junction protein of the intestinal epithelial cells is lowered, the tight junction barrier of the intestinal epithelial cells is opened, and therefore the bioavailability of the MSC-CM is improved, and the role of treating POI is played.

The present invention relates to a composition. This composition contains a conditioned medium obtained by in-vitro culturing of mononuclear cell fraction.

Provided herein is a composition comprising a population of mesenchymal stem cell (MSC) aggregates, wherein the aggregates have a mean diameter between 30 to 250 microns and number of cells per aggregate between 100 to 500 MSCs. Also provided is a method of producing said composition, and a method of obtaining conditioned medium from said aggregates. Both the composition comprising the aggregates, and the conditioned medium may be used to treat diseases, disorders, conditions or injuries such as age-related disorders, chronic and acute inflammatory conditions (including arthritis), degenerative joint diseases (including osteoarthritis), joint injury, chronic and acute respiratory diseases, musculoskeletal conditions, neurodegenerative diseases and / or brain injury.

The invention provides an in vitro method for producing an isolated mammalian primitive retina stem cell (pRSC), comprising: (a) culturing an isolated embryonic stem cell (ESC) from a mammal in a cell culture medium that is free of feeder cells, feeder layer conditioned medium, or serum to produce and grow a culture of the isolated ESC; and (b) contacting the culture of the isolated ESC so grown with one or more of a Wnt or TGF-beta / BMP signaling inhibitor to differentiate the isolated ESC of (a) into a primitive retina stem cell, thereby producing an isolated mammalian pRSC.