196 results about "Stem cell culture" patented technology

The purpose of the present invention is to provide: a novel therapeutic or prophylactic agent for xerophthalmia; a therapeutic or prophylactic agent for graft-versus-host disease; a corneal epithelium wound healing promoter; a corneal invasive inflammatory cell inhibitor; or an inhibitor of differentiation of lymphocytes into effector T cells. The present invention provides: a therapeutic or prophylactic agent for xerophthalmia, a therapeutic or prophylactic agent for graft-versus-host disease, a corneal epithelial wound healing promoter, a corneal invasive inflammatory cell inhibitor, a tear amount maintenance agent, a corneal epithelial damage inhibitor, and a method for preparing the same, which comprises, as an active ingredient, a substance derived from a stem cell culture supernatant; a cell proliferation marker positive cell inhibitor, or an inhibitor of differentiation of lymphocytes to effector T cells.

The invention discloses a therapeutic nano-vesicle preparation rich in mitochondrial functional protein, a preparation method of the therapeutic nano-vesicle preparation and application of the therapeutic nano-vesicle preparation in treatment of radioactive skin injury. The preparation method comprises the following steps: culturing, amplifying and cleaning umbilical cord mesenchymal stem cells, dispersing the umbilical cord mesenchymal stem cells in a suspension, mechanically extruding the umbilical cord mesenchymal stem cells into a crude solution through a multistage aperture polycarbonate membrane, and centrifuging, concentrating and purifying the crude solution to obtain the preparation. The preparation has the remarkable advantages that local administration can be realized, inflammation can be quickly inhibited, oxidative stress is reduced, and a repair pathway is activated, so that the preparation is suitable for treating acute radioactive skin injury; the preparation method is easy to standardize and scale, high in stability and convenient for emergency storage and delivery, and avoids the defects of cell therapy; a plurality of targets such as immune inflammation, oxidative stress and the like can be synergistically regulated, the biological effect of hUMSCs is simulated and partially replaced, and the limitation caused by the cell survival rate and the microenvironment dependency is avoided; the key function of hUMSCs is covered in function, and the clinical feasibility, safety and emergency suitability of the application level are higher.

The invention discloses a cell fate distinguishing and differentiation prediction method based on a three-dimensional form, and belongs to the technical field of cell fate distinguishing and differentiation prediction. In order to solve the problem that cell fate differentiation is difficult to distinguish and predict under a small sample size, the method comprises the following steps: acquiring a living biological sample, and imaging by using a three-dimensional delay microscope to obtain cell three-dimensional image data; segmenting the cell membrane boundary and extracting geometric and high-order three-dimensional morphological characteristic parameters; labeling cell fate and differentiation states based on means such as cell lineage tracking or gene fluorescence labeling; and finally, determining an optimal morphological index and a minimum sample size through single-side double-sample t test, and applying the optimal morphological index and the minimum sample size to two new cell populations and outputting a cell fate distinguishing and differentiation prediction result. The method can realize cross-species, low-loss, low-cost and high-precision cell fate differentiation and differentiation prediction, and is suitable for development research, stem cell culture and disease diagnosis.

The invention relates to the technical field of rhinitis spray preparation, in particular to a method for preparing allergic rhinitis spray from umbilical cord mesenchymal stem cell exosomes, which comprises the following steps: 1, umbilical cord treatment; 2, culturing umbilical cord mesenchymal stem cells; 3, preparing an umbilical cord mesenchymal stem cell exosome; and 4, preparing the rhinitis spray. The rhinitis spray prepared by the method disclosed by the invention is simpler and more convenient to use. The exosome, the platelet growth factor and other substances in the rhinitis spray well solve the core problems of single curative effect, large side effect, low compliance and the like in the traditional rhinitis treatment, and through the induction and repair functions of the stem cell exosome and the bactericidal ability of the antibacterial peptide, the nasal cavity environment is improved, the anaphylactic reaction is reduced, the nasal mucosa is repaired, and the rhinitis treatment effect is improved. The allergic rhinitis is expected to be fundamentally treated.

The invention provides an extraction method of umbilical cord mesenchymal stem cell exosomes. The extraction method comprises the following steps: S1, collecting umbilical cord mesenchymal stem cell culture supernatant; s2, centrifuging the culture supernatant under a centrifugal force of 250-350 g, and collecting the supernatant to obtain a first mixed solution; s3, centrifuging the first mixed solution under a centrifugal force of 1500-2500 g, and collecting a supernatant to obtain a second mixed solution; s4, centrifuging the second mixed solution under a centrifugal force of 9000-11000 g, and collecting a supernatant to obtain a third mixed solution; and S5, filtering the third mixed solution, centrifuging under the centrifugal force of 90000-110000 g, collecting the precipitate, adding a PBS buffer solution, centrifuging under the centrifugal force of 90000-110000 g, and collecting the precipitate to obtain the umbilical cord mesenchymal stem cell exosome. The umbilical cord mesenchymal stem cell exosome with the purity up to 99% is extracted from the culture supernatant through twice low-speed centrifugation and three times of high-speed centrifugation.

The invention provides a serum-free stem cell culture medium and a preparation method thereof, and the culture medium realizes long-term stable culture of stem cells under a completely serum-free condition by optimizing basic culture medium components and adding a specific growth factor combination, an extracellular matrix simulant and a metabolism regulator. The culture medium comprises a basic culture medium, a recombinant human protein substitute, a cell adhesion promoting factor, a growth factor combination, an antioxidant and a metabolism regulator. Compared with the prior art, the invention has the following advantages: 1) animal-derived components are completely avoided, and the immunogenicity and the difference between batches are reduced; 2) maintaining the dryness of the stem cells through a specific growth factor combination; 3) adding a metabolism regulator to optimize cell energy metabolism; 4) the cost is obviously lower than that of commercially available like products; and 5) supporting long-term culture of stem cells without spontaneous differentiation. The invention also provides a preparation method of the culture medium and a method for culturing stem cells by using the culture medium.

The invention discloses a mechanical response type engineered mesenchymal stem cell exosome preparation for treating diseases caused by fibrosis inflammation, a preparation method and application, and the exosome preparation is an exosome which is secreted by mesenchymal stem cells and has high cholesterol and high-efficacy protein. The exosome is prepared by culturing mesenchymal stem cells on a hydrogel matrix in a modulus hardness range of 1-50KPa, the exosome secretion amount of a single cell is (3-5) * 10 < 4 > / cell, the cholesterol content is (1-10) nM / 5mg exosome, and the size of the exosome is 110-140nm; the high-efficacy protein is a key functional protein for regulating and controlling the biological activity of the exosome and the cell action efficiency. The cholesterol content in the exosome is precisely regulated and controlled, the cytoplasm delivery efficiency and tissue penetrability of the exosome are improved, a whole-process control system of hydrogel preparation, stem cell culture and exosome purification is constructed, and the stability of the preparation and the yield of the exosome are improved.

The invention relates to the technical field of cell culture, and discloses a stem cell differentiation degree identification method based on image features. The method comprises the following steps: continuously capturing multi-temporal image data in a stem cell culture process through a high-resolution microscopic imaging system, and generating an image and environment synchronous data set; processing the data set, extracting dynamic morphological features and texture change modes of the stem cells, and constructing a fusion feature set; outputting a differentiation process index based on the fusion feature set; a differentiation degree grade division rule is set, and a differentiation process index and differentiation grade mapping table is established; in a real-time application stage, acquiring real-time imaging data and environment readings, and inputting the real-time imaging data and the environment readings into an evaluation network to calculate real-time differentiation process indexes; querying the mapping table according to the real-time index to obtain a differentiation grade judgment result; comparing the judgment result with a preset target range, and if the judgment result falls into the target range, automatically executing differentiation state marking and culture parameter adjusting operation.

The invention provides a stem cell culture process information abnormity early warning method, which comprises the following steps: preprocessing original data in a stem cell culture process, extracting layered features to obtain basic statistical features, time sequence trend features and environmental factor interaction features, and updating the features in real time through a dynamic sliding window, after the verified feature set is obtained, fusion calculation is carried out to obtain a multi-dimensional fusion feature set; calculating a trend deviation degree, a feature correlation degree and a fluctuation dispersion degree of the multi-dimensional fusion feature set, and carrying out dynamic weight distribution and fusion to obtain a fusion anomaly degree value; and matching the fusion anomaly degree value with a multi-level threshold value to obtain a matching result, judging an abnormal state type in combination with an association relationship between the feature anomaly type and the cultivation stage, and generating an anomaly early warning result. According to the method, through data preprocessing, layered feature extraction and fusion and dynamic anomaly calculation, the accuracy, real-time performance and practicability of stem cell culture anomaly early warning are improved.

The invention discloses a bionic bone marrow microenvironment assembly for hematopoietic stem cell in-vitro amplification and a preparation method. The bone marrow microenvironment assembly comprises a three-dimensional scaffold and osteoblasts (OBs), wherein the three-dimensional scaffold comprises chitosan (CS) and sodium alginate (Alg) which are prepared into mixed gel, and the mixed gel is freeze-dried to prepare the three-dimensional scaffold; wherein the osteoblasts (OBs) are obtained by culturing and induced differentiation of umbilical cord mesenchymal stem cells (MSCs) inoculated on a three-dimensional scaffold, and the osteoblasts (OBs) as stromal cells and the three-dimensional scaffold jointly form the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly; in the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly, the preparation of the three-dimensional scaffold comprises the processes of freezing, remelting, freezing, crosslinking, freeze-drying and the like, so that a three-dimensional space form with wrinkles on the pore surface and pore size distribution between 70 microns and 400 microns is generated in the three-dimensional scaffold, and the three-dimensional space form is closer to a real stem cell niche; therefore, the practical application effect of the bionic bone marrow microenvironment assembly for in-vitro amplification of the hematopoietic stem cells is further improved.

The invention belongs to the field of stem cell culture, and relates to a pluripotent stem cell culture medium, which comprises an E6 basal culture medium, 2 ng / mL of TGF-beta1, 0-50 ng / mL of FGF-2, and 0.01-1 [mu] M of SUN11602. According to the technical scheme, proliferation and pluripotency of the hPSC are effectively maintained in a mode of combining small chemical molecules with low-concentration FGF-2.

This invention provides a method for propagating pluripotent stem cells (PSCs) by suspension culture in a bioreactor. [Solution] The method comprises the following steps: (i) adding a ROCK inhibitor (ROCKi) to pluripotent stem cells cultured in suspension in a bioreactor; (ii) adding a cell dissociation agent, thereby dissociating aggregates of pluripotent stem cells; (iii) diluting the cell dissociation agent added in step (ii) by adding a sufficient excess volume of culture medium to reduce the concentration of the cell dissociation agent to a concentration at which cell aggregates can be re-formed; and (iv) culturing the mixture obtained in step (iii) under appropriate conditions that allow for the proliferation of PSCs.

The invention discloses a culture medium for adipose-derived stem cells, and particularly relates to the technical field of stem cell culture. The culture medium comprises a basic culture medium and nerve-vascular niche components, wherein the basic culture medium comprises DMEM / F12, 10% of human-derived platelet lysate and 1% of antibiotic solution; the nerve-vascular niche comprises the following components: a Slit3 recombinant protein, a nerve growth factor-beta, a glial cell-derived neurotrophic factor, a lysyl oxidase inhibitor, dexamethasone, 3-isobutyl-1-methylxanthine and insulin. The traditional fetal calf serum is replaced by the human-derived platelet lysate, so that the risks of immunological rejection and pathogen pollution are avoided; through the synergistic effect of nerve-vessel niche factors, blood vessel maturation and innervation are promoted, programmed mechanical stretching culture is combined, the adipogenic differentiation efficiency of adipose-derived stem cells is remarkably improved, and engineered adipose tissue which is huge in lipid droplet, compact in structure and provided with a nerve-vessel network is formed.

The invention discloses a plant stem cell nutrition synergist, and belongs to the technical field of plant stem cell nutrient solutions. The nutrient synergist can significantly improve the proliferation rate and survival rate of stem cells, promotes differentiation of the stem cells to target cell types, plays an important role in stem cell culture, can significantly improve the culture efficiency of the stem cells, provides comprehensive nutrient support and a suitable growth environment for stem cell culture, and has good application prospects. Proliferation and differentiation of the stem cells are promoted, and further research on the plant stem cells is facilitated.

The utility model relates to the technical field of stem cell culture, and discloses efficient stem cell sampling equipment which comprises a sampling box, the top of an assembly cavity is fixedly connected with a mounting plate, a sampling barrel is embedded in the center of the mounting plate, a rotating mechanism is arranged in the center of the sampling barrel, and the rotating mechanism is connected with the mounting plate. The top of the rotating mechanism is connected with a containing mechanism, the containing mechanism comprises a containing disc, clamping grooves, a first sampling assembly, a mounting groove and a second sampling assembly, the multiple clamping grooves are formed in the circumference of the edge of the containing disc at equal intervals, the first sampling assembly is connected into the clamping grooves in a clamped mode, and the second sampling assembly is connected into the mounting groove in a clamped mode. According to the equipment, the accommodating mechanism is arranged, the sampling assembly I and the sampling assembly II are used for stably placing the sampling tube and the sampling box on the accommodating disc, and the accommodating disc is driven by the rotating mechanism to rotate so as to realize sampling, so that the adaptability of the equipment to different sampling containers is improved, and the equipment is convenient to operate and replace parts.

The invention discloses a stem cell constant temperature device, and belongs to the technical field of stem cell storage, the stem cell constant temperature device comprises a cabinet body, the lower side in the cabinet body is provided with a partition part, the partition part comprises a fixed cylinder fixedly connected to the lower side in the cabinet body, the upper side in the fixed cylinder is provided with a material gathering cavity, and the left side and the right side in the fixed cylinder are hinged to baffles; according to the stem cell culture cabinet, the baffle is arranged to separate the fixed cylinder from the inner space of the cabinet body after stem cells and culture solution enter the deep position of the fixed cylinder, and the space for collecting the stem cells and the culture solution is separated from the inner cavity of the cabinet body by the baffle; according to the stem cell storage cabinet, the contact time of stem cells and a culture solution and air in the cabinet body is effectively shortened, the phenomenon that the storage effect of other stem cells is affected due to temperature rise of a gas phase area is avoided, meanwhile, the leaked stem cells and the culture solution can be collected in time through the material collecting cavity, and the stem cells and the culture solution are prevented from damaging temperature control equipment in the cabinet body.

The utility model discloses a portable preservation box for stem cell culture, which comprises a box body, the inside of a mounting shell is divided into circulation channels through a plurality of groups of placing boxes, and the circulation channels are used for prolonging the forced contact time of air and dry ice; the air inlet end of the air pump communicates with two sets of extraction pipes used for extracting air to flow in the circulation channel. Through the design of the mounting shell, the placement box, the air pump, the extraction pipe, the exhaust pipe and the like, and through the cooperation of the air pump, the extraction pipe and the exhaust pipe, air in the box body can quickly flow in the mounting shell, and the air is forced to be in contact with dry ice in the placement box when flowing in the mounting shell, so that the air is quickly cooled and uniformly circulated; therefore, a cold source can be utilized to the maximum extent, energy waste is reduced, a set low-temperature environment can be recovered in a short time through an active refrigeration mode, the time for exposing stem cells to non-ideal temperature is shortened, and cell activity decline or cell death is avoided.

The present application relates to the technical field of biology, and particularly relates to a mesenchymal stem cell culture medium and a preparation method and application thereof.The mesenchymal stem cell culture medium comprises a basic culture medium, fetal bovine serum, basic fibroblast growth factor, polymyxin B and deionized water.The present application can simultaneously improve the proliferation activity of mesenchymal stem cells and the secretion performance of HGF by simultaneously adding the basic fibroblast growth factor and the polymyxin B in the basic culture medium; more stem cells can be in the active proliferation and division process by limiting the content of the basic fibroblast growth factor in the mesenchymal stem cell culture medium to 5-50 ng / mL; and the secretion performance of HGF can be improved while the cell proliferation activity is maintained by limiting the content of the polymyxin B in the mesenchymal stem cell culture medium to 50-100 mu g / mL.

The invention discloses a stem cell culture all-in-one machine, and particularly relates to the related technical field of stem cell culture.The stem cell culture all-in-one machine comprises a culture box, a warm air blower and a temperature detector, the warm air blower and the temperature detector are installed above the culture box, and a box door is rotationally connected to the front portion of the culture box through a pin shaft; a moving assembly used for assisting a worker to take and place culture dishes is arranged in the incubator, a cleaning assembly used for sterilizing and disinfecting the interior of the incubator is arranged below the moving assembly, and a sliding tripod can be driven to slide in multiple sets of sliding grooves formed in a matching box in the rotating process of a chain; the sliding tripod moves to drive the mounting box to move, so that a worker can conveniently take and place a specified culture dish, the stability of the culture dish in the taking and placing process can be improved, and the phenomenon of rollover, pollution to the interior of the culture box and influence on the subsequent cell culture environment are avoided.

According to the stem cell culture solution pollution-free collection module, the sterile isolation and vacuumizing process of the internal environment of a collection bottle is achieved through a collection assembly, the function that air in the bottle is pumped out firstly and then the stem cell culture solution is injected is achieved through the coaxial inner needle and outer needle design of an infusion assembly, and the stem cell culture solution is collected through a multi-stage self-sealing structure after the needles are pulled out. And the collection bottle is automatically closed and sealed after liquid injection is completed, so that the pollution-free stem cell culture solution collection module which is pollution-free in the whole process and is closed and safe is realized. The device is characterized in that the device comprises a collecting assembly and an infusion assembly, the infusion assembly is inserted into the collecting assembly, the infusion assembly injects a stem cell culture solution into the collecting assembly, and the collecting assembly comprises a collecting bottle, a sealing plug, a disposable sterile unsealing film, a duckbill valve, a check valve and a multi-layer self-resetting diaphragm; and the sealing plug is clamped at the opening end of the collecting bottle.

The invention discloses a serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and application thereof. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition is prepared from the following components in parts by mass: 15 to 25 parts of platelet-derived growth factor-BB; 15 to 25 parts of basic fibroblast growth factors; 8 to 12 parts of transforming growth factor-beta; and 8-12 parts of recombinant human insulin. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and the application thereof disclosed by the invention have the following beneficial effects: (1) the proliferation efficiency is remarkably improved; (2) the cell morphology and dryness can be well maintained; and (3) the compliance and the compatibility are good.

Disclosed is a chemically defined airway basal stem cell culture medium. The provided culture medium involves a simple preparation method and high safety, and does not require a feeder layer or a matrigel-coated culture plate. By means of the culture medium, primary cultured airway basal stem cells achieve good stability; and after multiple passages, the normal morphology of the basal stem cells can be maintained and good differentiation potential can be maintained. In addition, the culture medium has clear components, and can meet both scientific research-grade and clinical-grade applications of airway basal stem cells.

The application discloses a kind of stem cell culture devices, belong to stem cell technical field, including sealed incubator, syringe, the inside of the sealed incubator is equipped with electric rotating table, the top of the electric rotating table is equipped with adjustable shaking assembly, the adjustable shaking assembly is equipped with petri dish carrier, petri dish limiting component, the petri dish limiting component is equipped with uniform feeding assembly, the top of the sealed incubator is equipped with liquid injection pipe, the top of the liquid injection pipe is equipped with sealed iris component, the outside of the sealed incubator is equipped with lifting assembly.The adjustable shaking assembly, petri dish limiting component, uniform feeding assembly of being equipped with, so that petri dish realizes multidimensional periodicity shaking, promotes stem cell to adhere to the growth and material exchange;Sealed iris component, syringe are set, in the process of injecting reagent, the sealing of incubator is always maintained, avoid exposing sample in air, guarantee the stability of culture environment.

A reagent, a drug source material, a drug, a quasi-drug, a cosmetic source material, or a cosmetic that includes a supernatant of a culture solution of stem cells and is for reducing skin redness or inflammation, inflammation, number of pores, pore size, skin spots, and sebum. A reagent, a drug source material, a drug, a quasi-drug, a cosmetic source material, or a cosmetic that includes a secretion of stem cells and is for reducing skin redness or inflammation, inflammation, number of pores, pore size, skin spots, and sebum. The secretion of the stem cells may be extracellular vesicles. The secretion of the stem cells may be exosomes.

The invention relates to an adipose-derived stem cell culture medium containing wormwood extract and a preparation method of the adipose-derived stem cell culture medium, and belongs to the technical field of biology. The adipose-derived stem cell culture medium contains a DMEM (Dulbecco Modified Eagle Medium) basal culture medium, and also contains the following components: 5 to 7 [mu] g / mL of wormwood extract, 1 to 1.5 [mu] g / mL of phosphatidylserine, 0.02 to 0.03 [mu] g / mL of D-calcium pantothenate, 1.2 to 1.5 [mu] g / mL of sialic acid, 0.06 to 0.08 [mu] g / mL of eucommia male flower peptide, 0.3 to 0.35 [mu] g / mL of transferrin and 0.3 to 0.4 mg / mL of bovine serum albumin. The prepared adipose-derived stem cell culture medium has a good promoting effect on proliferation of adipose-derived stem cells from rabbits and mice, the added wormwood polysaccharide extract has a good bacteriostatic effect, and the safety and stability of the culture medium can be effectively maintained.

The invention discloses a novel ginseng stem cell culture system which comprises a basic culture medium and a growth regulation composition, and the basic culture medium comprises ammonium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate and calcium chloride; the growth regulating composition comprises 2, 4-dichlorphenoxyacetic acid, zeatin, salicylic acid and natural polysaccharide. The basic culture medium also comprises casein hydrolysate, coconut water extract and cane sugar. The composition also comprises an anti-oxidation composition. The invention further discloses a disposable flexible bioreactor for ginseng stem cell culture, the bioreactor comprises a tank body made of a flexible material, an air distribution device, a sterile sampling device and an online parameter monitoring device, and the top of the tank body structure is a cylindrical body and is provided with an inoculation opening, a culture medium liquid supplementing opening, an air outlet and a sampling opening; the bottom is an inverted cone provided with a parameter monitoring device interface and an air inlet, and the air inlet is connected with a ventilation device and an air flow adjusting device. The invention also discloses application of the culture system and the bioreactor.

To provide compositions containing RPE cells or RPE monolayers, as well as methods and materials for making RPE cells or RPE monolayers from, for example, stem cells (e.g., iPSCs).SOLUTION: Disclosed is a method for making a retinal pigment epithelium monolayer, the method comprising, or consisting essentially of, culturing stem cells in a container having a surface coated with fibrinogen, wherein the surface is coated with greater than 3 μg / mL of fibrinogen, wherein the cells are in contact with the fibrinogen, and wherein the cells form the retinal pigment epithelium monolayer.SELECTED DRAWING: None

1. The name of the design product: stem cell incubator. 2. The use of the design product: stem cell culture in the field of biology. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

This utility model discloses a culture device for mesenchymal stem cell therapy of osteoarthritis, belonging to the field of culture devices. It includes a culture vessel with an opening at the top; a viewing window embedded in the top of the culture vessel; a rotating mechanism and a support member, respectively disposed on the bottom wall of the inner cavity of the culture vessel; several support members, respectively disposed on the outer wall of the rotating mechanism; culture dishes are placed on the support members; the rotating mechanism drives the support members and culture dishes to rotate; the support members lift the culture dishes on the corresponding support members to below the viewing window; and a driving member, penetrating the side wall of the culture vessel and connected to the support members, provides power to the support members. This utility model's culture device for mesenchymal stem cell therapy of osteoarthritis reduces the chance of direct contact between the culture dishes and the outside environment without affecting the observation of the culture dishes, thus better protecting the stem cell culture environment.

The utility model relates to the technical field of culture dishes, in particular to an umbilical cord stem cell culture dish convenient to store and take, which comprises a box body, a plugging cover, a first connecting plate and a second connecting plate, the upper end of the box body is connected with the plugging cover in a clamping manner, the plugging cover is connected with a sealing mechanism, one side of the box body is connected with the first connecting plate, and the second connecting plate is connected with the second connecting plate. The other side of the box body is connected with a second connecting plate, a first limiting mechanism is arranged on the side edge of the first connecting plate, and a clamping mechanism is arranged on the side edge of the second connecting plate. In the using process, the plugging cover is connected with the box body in a clamped mode, then the bolt is rotated to drive the plugging plate to ascend to suck air in the box body, so that the plugging cover and the box body are clamped more tightly, the sealing performance of the device is improved, and when the plugging cover needs to be taken down, the plugging block is taken down at the moment; and then the gas enters the box body through the circular groove and the circular column, so that the plugging cover can be easily taken down and is convenient to store and take.