437 results about "Stem cell culture" patented technology

The present invention provides a serum-free culture medium for in vitro suspension culture of porcine muscle stem cells and its application, which can help porcine muscle stem cells expand and be suspended under serum-free and carrier-free conditions. Compared with traditional porcine muscle stem cell culture protocols and culture media, this serum-free suspension culture medium enables porcine muscle stem cells to be cultured continuously at high densities in both batch and fed-batch culture processes under serum-free and carrier-free suspension culture conditions, while maintaining high cell viability and achieving a peak cell density of 1×10 7 The beneficial effect of the present invention is to effectively solve the problem of serum-free and carrier-free efficient suspension expansion of cell cultured meat seed cells, and provide a technical and raw material basis for the industrial production of cell cultured meat.

The invention relates to the field of remote monitoring, in particular to a stem cell culture process monitoring method based on the Internet of Things. Comprising the steps that environmental parameters are collected, sensor data are obtained, the sensor data are verified through a median filtering algorithm, and whether the sensor data are abnormal or not is judged; based on the sensor data marked as normal, calculating by using a nonlinear dynamic coupling analysis algorithm to obtain an environment state index; based on the environment state index, using a non-linear state monitoring prediction algorithm to predict and obtain an environment state index at a future moment; based on the predicted environmental state index of the future moment, using an intelligent early warning trigger algorithm based on environmental state prediction to obtain an early warning state, and generating an early warning signal according to the early warning state; and based on the early warning signal, a dynamic feedback adjustment algorithm is used to automatically generate a control signal. The technical problem that normal operation is interfered due to the fact that a wrong early warning signal is easily triggered by misjudgment when the environment state changes critically is solved.

The invention provides a whole-process intelligent management system for stem cell culture medium development, and belongs to the technical field of intelligent management. According to the method, artificial intelligence, big data analysis and knowledge graph technologies are fused, and the whole process from demand analysis, formula intelligent design and virtual simulation, small-scale culture verification and iterative optimization, pilot scale-up process optimization to quality compliance and tracing is covered. All the modules are coordinated through the central intelligent decision engine, the research and development efficiency of the culture medium can be remarkably improved, the development cost can be greatly reduced, and batch consistency and quality stability of the culture medium are ensured by reducing trial and error and optimizing in real time. Meanwhile, a full-chain electronic tracing function is provided, the requirements of laws and regulations for compliance are comprehensively met, and the problems that traditional research and development are low in efficiency, high in cost, unstable in batch and the like are effectively solved.

The invention discloses stem cell culture equipment, and relates to the technical field of cell culture equipment, the stem cell culture equipment comprises a culture box, a rotating disc and a culture dish, horizontal plates are arranged in the middle of the culture box at equal intervals, and the middle of each horizontal plate is connected with the rotating disc through a bearing. The stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, a limiting ring and a circular track are separated, a conical block drives a tray to push culture dishes to be rapidly taken and placed, a rotating disc rotating slowly facilitates personnel to take or place the culture dishes one by one at the position close to an opening of a culture box, the limiting ring and the circular track are in matched butt joint, and the operation is convenient. The extrusion part elastically extrudes the culture dish on the tray when reversely moving and overturning, and meanwhile, the traction balance wheel is propped against the upper edge of a sealing end cover of the culture dish, so that the culture equipment can prevent the culture dish which is rotationally placed from shaking and shifting, and can be used for lifting and ejecting all the culture dishes at one time when the culture dish is taken out; and the operation of manually placing the culture dishes can also be reduced.

The invention provides an autologous fat-derived mesenchymal stem cell composition as well as a preparation method and application thereof, and belongs to the technical field of medical auxiliary materials. The method comprises the following steps: separating mesenchymal stem cells from autologous fat particles, culturing in a stem cell culture medium added with cell stimulation particles and fetal calf serum, separating to obtain stem cells and an extracellular matrix, preparing the stem cells into stem cell lipidosome, and preparing the stem cell lipidosome into the mesenchymal stem cells. Sanguinarine and curcumin are embedded in a composite shell material of chitosan and beta-cyclodextrin to prepare the sustained release microspheres. The mesenchymal stem cell composition of the autologous fat source prepared by the invention has good air permeability, softness, certain mechanical property and good biocompatibility, can quickly promote wound healing, is convenient to remove, and does not cause secondary injury.

The invention relates to the technical field of stem cell culture, and one embodiment of the invention provides a stem cell differentiation culture device which comprises a culture box and further comprises an air inlet pipe, a main fan, a fixed box, a conversion cylinder, a filter cylinder, an auxiliary fan, a discharge box and a cleaning mechanism, the culture box is communicated with a temperature adjusting system through the air inlet pipe, the fixed box is communicated with the air inlet pipe, and the air inlet pipe is communicated with the main fan. The conversion cylinder is rotatably arranged in the fixed box, the interior of the conversion cylinder is divided into two conversion cavities, the filter cylinder is fixedly connected to the interior of the conversion cavities and communicates with the air inlet, the auxiliary fan is installed in the filter cylinder, the discharge box communicates with the fixed box through the discharge port, and the discharge box communicates with a discharge pipe. The cleaning mechanism is used for being matched with the auxiliary fan to clean the inner wall of the filter cartridge. By means of the technical scheme, the technical problem that dust accumulates on a filter screen in a ventilation system in the prior art, and regular disassembly and cleaning are needed is solved.

The invention belongs to the technical field of stem cell culture and exosome extraction, and provides an RELM beta pre-activated stem cell outer vesicle with high anti-inflammatory and anti-fibrosis activity. According to the method, the mesenchymal stem cells are subjected to hunger culture after being adhered to the wall and then are continuously cultured by a complete culture medium containing RELM beta factors, then supernate is collected, and the exovesicles are obtained through separation. In the preparation process of the extracellular vesicles, the paracrine function of MSC cells is promoted by the RELM beta, the secretion of anti-inflammatory and anti-fibrosis active factors is obviously activated, and the generation of the extracellular vesicles with enhanced anti-inflammatory and anti-fibrosis effects is promoted. Gene modification is not involved, toxic biochemical reagents or materials are not added, exogenous operation steps are simple and convenient, clinical application is guaranteed through sterile operation, no RELM beta residues exist after extracellular vesicle extraction, and the method is safe, non-toxic and effective.

The purpose of the present invention is to provide: a novel therapeutic or prophylactic agent for xerophthalmia; a therapeutic or prophylactic agent for graft-versus-host disease; a corneal epithelium wound healing promoter; a corneal invasive inflammatory cell inhibitor; or an inhibitor of differentiation of lymphocytes into effector T cells. The present invention provides: a therapeutic or prophylactic agent for xerophthalmia, a therapeutic or prophylactic agent for graft-versus-host disease, a corneal epithelial wound healing promoter, a corneal invasive inflammatory cell inhibitor, a tear amount maintenance agent, a corneal epithelial damage inhibitor, and a method for preparing the same, which comprises, as an active ingredient, a substance derived from a stem cell culture supernatant; a cell proliferation marker positive cell inhibitor, or an inhibitor of differentiation of lymphocytes to effector T cells.

The invention discloses a therapeutic nano-vesicle preparation rich in mitochondrial functional protein, a preparation method of the therapeutic nano-vesicle preparation and application of the therapeutic nano-vesicle preparation in treatment of radioactive skin injury. The preparation method comprises the following steps: culturing, amplifying and cleaning umbilical cord mesenchymal stem cells, dispersing the umbilical cord mesenchymal stem cells in a suspension, mechanically extruding the umbilical cord mesenchymal stem cells into a crude solution through a multistage aperture polycarbonate membrane, and centrifuging, concentrating and purifying the crude solution to obtain the preparation. The preparation has the remarkable advantages that local administration can be realized, inflammation can be quickly inhibited, oxidative stress is reduced, and a repair pathway is activated, so that the preparation is suitable for treating acute radioactive skin injury; the preparation method is easy to standardize and scale, high in stability and convenient for emergency storage and delivery, and avoids the defects of cell therapy; a plurality of targets such as immune inflammation, oxidative stress and the like can be synergistically regulated, the biological effect of hUMSCs is simulated and partially replaced, and the limitation caused by the cell survival rate and the microenvironment dependency is avoided; the key function of hUMSCs is covered in function, and the clinical feasibility, safety and emergency suitability of the application level are higher.

The invention discloses a stem cell culture image identification and detection method, and relates to the technical field of stem cell image identification, and the method comprises the following steps: carrying out the graying processing of a stem cell image, obtaining a stem cell gray-scale image, carrying out the binarization processing of the stem cell gray-scale image, obtaining a stem cell binarization image, and obtaining a real-time complete contour based on the stem cell binarization image; acquiring a real-time length-width ratio; obtaining a range of a historical length-width ratio based on the first number of historical mesenchymal stem cells; the qualified real-time length-width ratio is obtained based on the range of the real-time length-width ratio and the historical length-width ratio, and the method is used for solving the problems that in an existing mesenchymal stem cell number recognition technology, the effective number of mesenchymal stem cells in the culture process cannot be accurately extracted, and consequently the accuracy and efficiency of recognizing the number of the mesenchymal stem cells are low; under the condition, the culture quality of the mesenchymal stem cells cannot be effectively judged.

The invention belongs to the technical field of stem cell exosome preparation, and provides a preparation method and therapeutic application of a stem cell exosome loaded with therapeutic molecules, and the preparation method comprises the following steps: S1, selecting and culturing a stem cell line with exosome secretion capability; s2, in the stem cell culture process, adding selected therapeutic molecules, so that the therapeutic molecules can be taken by the stem cells and integrated into exosomes of the stem cells; s3, collecting stem cell culture supernate, and separating and purifying the exosome through a differential centrifugation, ultrafiltration or density gradient centrifugation method; s4, identifying the exosome obtained by separation; a stem cell line with efficient exosome secretion capacity is selected and cultured, and selected therapeutic molecules are added in the culture process, so that efficient loading of the therapeutic molecules is realized; according to the method, the operation steps are simplified, the loading efficiency is improved, it is ensured that therapeutic molecules can be stably integrated into the stem cell exosome, and meanwhile the biological activity and stability of the exosome are kept.

The invention discloses a sheep formation state pluripotent stem cell culture medium and application thereof. The invention belongs to the technical field of biology, and particularly relates to a sheep formation state pluripotent stem cell culture medium and application thereof. The composition for culturing the sheep formation state pluripotent stem cells, disclosed by the invention, contains an N2B27 basic culture medium and cell factors, wherein the cell factors contain CHIR99021, IWR1, A419259, a recovery hub IL6, a recovery hub sIL6R, a recovery hub Activin A and a recovery hub FGF2 (Fibroblast Growth Factor 2). The culture medium prepared from the composition can obtain stable sfPSCs, and can be applied to construction of sheep gene editing model animals.

The invention provides a small extracellular vesicle composite collagen hydrogel injection, and belongs to the technical field of osteoarthritis, and the small extracellular vesicle composite collagen hydrogel injection is prepared by the following steps: S1, mixing a sodium alginate solution with a type I collagen solution to obtain a mixed solution, adding a CaCl2 solution into the mixed solution to form gel, adding an FGF18 solution into the gel, and mixing to obtain FGF18 sodium alginate / collagen hydrogel; s2, transfecting a Lenti-IGF-1-EGF lentiviral vector to P3 generation human umbilical cord mesenchymal stem cells, culturing to obtain P5 generation IGF-1 overexpressed MSCs cells, namely P5 generation IGF-1-MSCs cells, co-culturing with the P5 generation IGF-1-MSCs cells by using curcumin, and separating and extracting a small extracellular vesicle solution rich in curcumin; and S3, mixing the small extracellular vesicle solution rich in curcumin with the FGF18 sodium alginate / collagen hydrogel, so as to obtain the small extracellular vesicle composite collagen hydrogel injection. The invention provides a small extracellular vesicle composite collagen hydrogel injection, which achieves the purposes of relieving inflammatory injury of osteoarthritis and enhancing the treatment effect of osteoarthritis.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of a function-enhanced mesenchymal stem cell exosome, the preparation method comprises the following steps: S1, preparing an NK cell exosome, collecting supernate in an NK cell culture medium, and treating the supernate by using an optimized exosome separation and purification process to obtain an NK cell exosome; finishing preparation of a stock solution; s2, culturing the mesenchymal stem cells, culturing the cells in a 3D suspension culture mode, treating and controlling low oxygen concentration by combining with NK cell exosomes, and simulating an in-vivo inflammation microenvironment in a multi-azimuth manner, so that the exosomes secreted by the mesenchymal stem cells have a stronger immune regulation function and an anti-inflammatory effect; s3, preparation of mesenchymal stem cell exosomes: preparing a stock solution through an exosome separation and purification process combining a tangential flow ultrafiltration technology and a PS affinity chromatography technology; the exosome stock solution obtained by the method is high in purity and high in yield, and has more excellent biological activity and excellent treatment effect.

The invention discloses a perfusion device for stem cell culture, and belongs to the technical field of cell culture.The perfusion device comprises a frame, a closing assembly convenient to overhaul is arranged on the frame, a culture container is arranged in the frame, a perfusion bottle is fixedly installed in the frame, a filter box is fixedly installed in the frame, and the filter box is connected with the perfusion bottle. A pumping assembly is arranged in the frame, a penetrating groove is formed in the top of the filter box, and a connecting pipe is connected to the filter box; culture cells are conveniently collected through the arranged filter cylinder, the cells cultured in the perfusion bottle and a culture solution flow into the filter box through a liquid supply pipe, the filter cylinder is covered with a filter membrane, the liquid can flow to the outside to intercept the cells, then the cells can be filtered in the filter cylinder to be collected, then the filter cylinder is rotated, and the cells can be conveniently collected. The threaded sleeve is separated from the threaded head, so that the filter cartridge can be detached, the cells can be conveniently separated and collected, and the working efficiency is improved.

The present invention belongs to the field of cell culture media. Provided are a method for preparing a stem cell culture medium by using a protein hydrolysate as a core raw material and a method of using the stem cell culture medium. The stem cell culture medium contains 20-80% of the protein hydrolysate on the basis of the total weight of the components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high-free amino acid yeast hydrolysate, a high-nucleotide yeast hydrolysate, a high-glutathione yeast hydrolysate, a high-polypeptide yeast hydrolysate, a rice protein hydrolysate, a high-glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The preparation of a natural animal stem cell culture medium by using a whole nutrient protein hydrolysate as a core raw material can meet the nutritional requirements for animal stem cell proliferation under the conditions of no additional addition or addition of a small amount of amino acids, vitamins, growth factors and serum, which effectively reduces the cost of the stem cell culture medium, thereby providing the stem cell culture medium with wide application prospects in the field of foods such as cultured meat.

The invention relates to an N2B27 culture medium for inducing generation of bidirectional pluripotent stem cells, one of LY2090314, AS1842856 or CHIR99021 is added into an N2B27 basal culture medium, and the concentration of the CHIR99021 is 10 mu M. The invention also relates to a preparation method of the N2B27 culture medium. The weight of the LY2090314 is 10 nM, and the weight of the AS1842856 is 0.6 [mu] M. The invention also provides an application of the culture medium in induced production of bidirectional pluripotent stem cells. According to the invention, a novel stem cell culture condition for promoting co-expression of genes OCT4 and CDX2 by adding small molecules LY2090314 (LY), AS1842856 (AS) and CHIR99021 to inhibit signal channels of FOXO1 and GSK3 is screened out. The culture conditions are simple and convenient, the application range is wide, the cultured cells keep the characteristics of 16-32 cell stages of the embryos, and the development characteristics of the embryos can be efficiently reproduced. Under the condition of not depending on transcription factors, the screened bidirectional pluripotent stem cells (BPSCs) can promote efficient generation of TSC cells and establish a TSC cell line through independent differentiation of a serum culture medium or induced differentiation of a TSC culture medium. The obstacles between early embryo pedigree are broken, and the research on early embryo development is promoted.

The invention discloses a cell fate distinguishing and differentiation prediction method based on a three-dimensional form, and belongs to the technical field of cell fate distinguishing and differentiation prediction. In order to solve the problem that cell fate differentiation is difficult to distinguish and predict under a small sample size, the method comprises the following steps: acquiring a living biological sample, and imaging by using a three-dimensional delay microscope to obtain cell three-dimensional image data; segmenting the cell membrane boundary and extracting geometric and high-order three-dimensional morphological characteristic parameters; labeling cell fate and differentiation states based on means such as cell lineage tracking or gene fluorescence labeling; and finally, determining an optimal morphological index and a minimum sample size through single-side double-sample t test, and applying the optimal morphological index and the minimum sample size to two new cell populations and outputting a cell fate distinguishing and differentiation prediction result. The method can realize cross-species, low-loss, low-cost and high-precision cell fate differentiation and differentiation prediction, and is suitable for development research, stem cell culture and disease diagnosis.

The invention relates to the technical field of rhinitis spray preparation, in particular to a method for preparing allergic rhinitis spray from umbilical cord mesenchymal stem cell exosomes, which comprises the following steps: 1, umbilical cord treatment; 2, culturing umbilical cord mesenchymal stem cells; 3, preparing an umbilical cord mesenchymal stem cell exosome; and 4, preparing the rhinitis spray. The rhinitis spray prepared by the method disclosed by the invention is simpler and more convenient to use. The exosome, the platelet growth factor and other substances in the rhinitis spray well solve the core problems of single curative effect, large side effect, low compliance and the like in the traditional rhinitis treatment, and through the induction and repair functions of the stem cell exosome and the bactericidal ability of the antibacterial peptide, the nasal cavity environment is improved, the anaphylactic reaction is reduced, the nasal mucosa is repaired, and the rhinitis treatment effect is improved. The allergic rhinitis is expected to be fundamentally treated.

The utility model discloses a support structure for a stem cell culture bottle, mainly related to the field of culture bottle support. It includes a base, one end of the base is rotatably connected to a telescopic component, the end of the telescopic component is rotatably connected to a support plate for placing a culture bottle, the base is provided with a plurality of slots used in conjunction with the end of the support plate, the end of the support plate is provided with a baffle, a pressure plate is slidably connected to the support plate, the culture bottle is located between the baffle and the pressure plate, and a positioning column is provided between the pressure plate and the support plate. The beneficial effect of the utility model is that it can solve the technical problems of inconvenient operation and easy contamination when the culture bottle is tilted, realize effective support for the culture bottle when tilted, reduce the possibility of contamination caused by liquid spilling in the culture bottle, and ensure the convenience and safety of the culture bottle operation.

The invention provides an extraction method of umbilical cord mesenchymal stem cell exosomes. The extraction method comprises the following steps: S1, collecting umbilical cord mesenchymal stem cell culture supernatant; s2, centrifuging the culture supernatant under a centrifugal force of 250-350 g, and collecting the supernatant to obtain a first mixed solution; s3, centrifuging the first mixed solution under a centrifugal force of 1500-2500 g, and collecting a supernatant to obtain a second mixed solution; s4, centrifuging the second mixed solution under a centrifugal force of 9000-11000 g, and collecting a supernatant to obtain a third mixed solution; and S5, filtering the third mixed solution, centrifuging under the centrifugal force of 90000-110000 g, collecting the precipitate, adding a PBS buffer solution, centrifuging under the centrifugal force of 90000-110000 g, and collecting the precipitate to obtain the umbilical cord mesenchymal stem cell exosome. The umbilical cord mesenchymal stem cell exosome with the purity up to 99% is extracted from the culture supernatant through twice low-speed centrifugation and three times of high-speed centrifugation.

The present application relates to the technical field of biological medicine, and particularly relates to a stem cell culture method for promoting wound healing and application thereof. The stem cell culture method for promoting wound healing comprises the following steps: step one, providing umbilical cord-derived mesenchymal stem cells; step two, adding luteolin and baicalin as culture medium additives in a serum-supplemented culture medium, wherein the concentration of luteolin is 1-10 micromoles / L, and the concentration of baicalin is 1-10 micromoles / L; step three, inoculating the mesenchymal stem cells into the culture medium containing the additives for culture, and the culture condition is 34-40 DEG C, 4-6% CO2, and the culture time is 48-96 hours; step four, harvesting the cultured mesenchymal stem cells to obtain stem cells for promoting wound healing. The culture method can promote the stem cells to secrete various growth factors, significantly improve the secretion of PGE2 and VEGF-C growth factors, and make the wound healing rate of the stem cell treatment group reach 96%, which has a significant effect on treating diabetic foot ulcers.

The invention relates to the technical field of stem cell culture, in particular to a stem cell culture device and a method thereof.The stem cell culture device mainly comprises a culture box, a door plate is movably installed on the side face of the culture box in a penetrating mode, a supporting shaft is rotatably installed on the inner wall of the lower end of the culture box through a damping bearing, and a placing plate and a dosing mechanism are fixedly installed at the upper end of the supporting shaft; the dosing mechanism is arranged above the placing plate, and the rotating mechanism is located in the incubator. An electric push rod is started to drive a disc to move upwards, and a piston rod and a piston are driven to move upwards, so that a conveying cylinder absorbs a culture solution from a storage box through a connecting pipe, the electric push rod is started to drive the lower piston to move downwards, the piston pushes the culture solution in the conveying cylinder to be conveyed into a culture dish from a liquid adding head through a conveying pipe, and a door plate does not need to be opened in the midway. Bacteria are prevented from entering a culture dish in culture, and stem cells in the culture dish are not easily polluted by external bacteria, so that the survival rate of the stem cells is ensured, and the culture quality of the stem cells is improved.

The invention relates to the technical field of bioengineering and digital twinning, and discloses a digital twinning modeling regulation and control system for a stem cell culture process, which comprises a signal acquisition module, a space-time coding module, a dynamic modeling module, a regulation and control decision module, a closed-loop execution module and an interactive interface module, according to the method, multi-modal data is collected through a composite sensor and coded into a space-time tensor, a gene noise-metabolic force field cross-scale dynamic model is constructed, a microenvironment regulation and control instruction is generated in combination with a multi-agent game, closed-loop cooperative regulation and control of a biochemical gradient field and mechanical parameters are achieved, and dynamic interactive calibration is achieved through a three-dimensional thermodynamic diagram. According to the method, a stem cell differentiation cross-scale dynamic model is constructed through multi-modal data fusion, microenvironment closed-loop regulation and control are realized by using space-time tensor coding and a multi-agent game strategy, and prediction precision and regulation and control real-time performance of a digital twin system are improved in combination with a three-dimensional visual interaction platform.

The invention relates to the technical field of data processing of stem cell culture equipment, and discloses a data processing system of stem cell culture equipment, which comprises the following modules: a data acquisition module generates a state and a control sequence by acquiring parameters in a stem cell culture process; the state modeling module constructs a multi-dimensional state matrix and a control matrix based on the collected data; the trend prediction module is used for constructing a state transition model and generating a prediction state vector at the next moment; the optimization control module solves a control strategy vector based on the prediction state vector; the control execution module is used for converting the control strategy vector into an actual control instruction; the closed-loop feedback module collects the actual state after adjustment and is used for correcting the state and the control matrix and dynamically updating the state transition model. According to the method, the state matrix and the control matrix based on the sliding time window are constructed, so that multi-dimensional time sequence information in the stem cell culture process is subjected to structured expression, and the effect of capturing the dynamic change trend of the environment in real time is achieved.

The invention provides a serum-free stem cell culture medium and a preparation method thereof, and the culture medium realizes long-term stable culture of stem cells under a completely serum-free condition by optimizing basic culture medium components and adding a specific growth factor combination, an extracellular matrix simulant and a metabolism regulator. The culture medium comprises a basic culture medium, a recombinant human protein substitute, a cell adhesion promoting factor, a growth factor combination, an antioxidant and a metabolism regulator. Compared with the prior art, the invention has the following advantages: 1) animal-derived components are completely avoided, and the immunogenicity and the difference between batches are reduced; 2) maintaining the dryness of the stem cells through a specific growth factor combination; 3) adding a metabolism regulator to optimize cell energy metabolism; 4) the cost is obviously lower than that of commercially available like products; and 5) supporting long-term culture of stem cells without spontaneous differentiation. The invention also provides a preparation method of the culture medium and a method for culturing stem cells by using the culture medium.

The invention discloses a mechanical response type engineered mesenchymal stem cell exosome preparation for treating diseases caused by fibrosis inflammation, a preparation method and application, and the exosome preparation is an exosome which is secreted by mesenchymal stem cells and has high cholesterol and high-efficacy protein. The exosome is prepared by culturing mesenchymal stem cells on a hydrogel matrix in a modulus hardness range of 1-50KPa, the exosome secretion amount of a single cell is (3-5) * 10 < 4 > / cell, the cholesterol content is (1-10) nM / 5mg exosome, and the size of the exosome is 110-140nm; the high-efficacy protein is a key functional protein for regulating and controlling the biological activity of the exosome and the cell action efficiency. The cholesterol content in the exosome is precisely regulated and controlled, the cytoplasm delivery efficiency and tissue penetrability of the exosome are improved, a whole-process control system of hydrogel preparation, stem cell culture and exosome purification is constructed, and the stability of the preparation and the yield of the exosome are improved.

The invention relates to the field of cell culture, in particular to a culture composition of stem cells and a culture method. The culture composition comprises NAD (Nicotinamide Adenine Dinucleotide) or a precursor thereof, flavonoid and coenzyme, and can be used for improving the proliferation capacity of stem cells and inhibiting the aging of the stem cells.

The invention belongs to the technical field of cell culture, and particularly relates to a serum-free mesenchymal stem cell culture medium and a preparation method thereof. The serum-free mesenchymal stem cell culture medium provided by the invention is prepared by taking a DMEM (Dulbecco Modified Eagle Medium) / F12 basal culture medium as a basis and adding supplementary factors, namely yeast hydrolysate, insulin, albumin, dexamethasone, taurine, salidroside, soy isoflavone, baicalein, human platelet lysate, momordica saponin and hyperoside. According to the serum-free mesenchymal stem cell culture medium provided by the invention, the normal form of cells can be maintained without supplementing serum, rapid proliferation is realized, and the biological characteristics of the mesenchymal stem cells are maintained.

The invention relates to the technical field of cell culture, and discloses a stem cell differentiation degree identification method based on image features. The method comprises the following steps: continuously capturing multi-temporal image data in a stem cell culture process through a high-resolution microscopic imaging system, and generating an image and environment synchronous data set; processing the data set, extracting dynamic morphological features and texture change modes of the stem cells, and constructing a fusion feature set; outputting a differentiation process index based on the fusion feature set; a differentiation degree grade division rule is set, and a differentiation process index and differentiation grade mapping table is established; in a real-time application stage, acquiring real-time imaging data and environment readings, and inputting the real-time imaging data and the environment readings into an evaluation network to calculate real-time differentiation process indexes; querying the mapping table according to the real-time index to obtain a differentiation grade judgment result; comparing the judgment result with a preset target range, and if the judgment result falls into the target range, automatically executing differentiation state marking and culture parameter adjusting operation.