339 results about "Stem cell culture" patented technology

The present invention provides a serum-free culture medium for in vitro suspension culture of porcine muscle stem cells and its application, which can help porcine muscle stem cells expand and be suspended under serum-free and carrier-free conditions. Compared with traditional porcine muscle stem cell culture protocols and culture media, this serum-free suspension culture medium enables porcine muscle stem cells to be cultured continuously at high densities in both batch and fed-batch culture processes under serum-free and carrier-free suspension culture conditions, while maintaining high cell viability and achieving a peak cell density of 1×10 7 The beneficial effect of the present invention is to effectively solve the problem of serum-free and carrier-free efficient suspension expansion of cell cultured meat seed cells, and provide a technical and raw material basis for the industrial production of cell cultured meat.

The invention provides a whole-process intelligent management system for stem cell culture medium development, and belongs to the technical field of intelligent management. According to the method, artificial intelligence, big data analysis and knowledge graph technologies are fused, and the whole process from demand analysis, formula intelligent design and virtual simulation, small-scale culture verification and iterative optimization, pilot scale-up process optimization to quality compliance and tracing is covered. All the modules are coordinated through the central intelligent decision engine, the research and development efficiency of the culture medium can be remarkably improved, the development cost can be greatly reduced, and batch consistency and quality stability of the culture medium are ensured by reducing trial and error and optimizing in real time. Meanwhile, a full-chain electronic tracing function is provided, the requirements of laws and regulations for compliance are comprehensively met, and the problems that traditional research and development are low in efficiency, high in cost, unstable in batch and the like are effectively solved.

The purpose of the present invention is to provide: a novel therapeutic or prophylactic agent for xerophthalmia; a therapeutic or prophylactic agent for graft-versus-host disease; a corneal epithelium wound healing promoter; a corneal invasive inflammatory cell inhibitor; or an inhibitor of differentiation of lymphocytes into effector T cells. The present invention provides: a therapeutic or prophylactic agent for xerophthalmia, a therapeutic or prophylactic agent for graft-versus-host disease, a corneal epithelial wound healing promoter, a corneal invasive inflammatory cell inhibitor, a tear amount maintenance agent, a corneal epithelial damage inhibitor, and a method for preparing the same, which comprises, as an active ingredient, a substance derived from a stem cell culture supernatant; a cell proliferation marker positive cell inhibitor, or an inhibitor of differentiation of lymphocytes to effector T cells.

The invention discloses a therapeutic nano-vesicle preparation rich in mitochondrial functional protein, a preparation method of the therapeutic nano-vesicle preparation and application of the therapeutic nano-vesicle preparation in treatment of radioactive skin injury. The preparation method comprises the following steps: culturing, amplifying and cleaning umbilical cord mesenchymal stem cells, dispersing the umbilical cord mesenchymal stem cells in a suspension, mechanically extruding the umbilical cord mesenchymal stem cells into a crude solution through a multistage aperture polycarbonate membrane, and centrifuging, concentrating and purifying the crude solution to obtain the preparation. The preparation has the remarkable advantages that local administration can be realized, inflammation can be quickly inhibited, oxidative stress is reduced, and a repair pathway is activated, so that the preparation is suitable for treating acute radioactive skin injury; the preparation method is easy to standardize and scale, high in stability and convenient for emergency storage and delivery, and avoids the defects of cell therapy; a plurality of targets such as immune inflammation, oxidative stress and the like can be synergistically regulated, the biological effect of hUMSCs is simulated and partially replaced, and the limitation caused by the cell survival rate and the microenvironment dependency is avoided; the key function of hUMSCs is covered in function, and the clinical feasibility, safety and emergency suitability of the application level are higher.

The invention discloses a sheep formation state pluripotent stem cell culture medium and application thereof. The invention belongs to the technical field of biology, and particularly relates to a sheep formation state pluripotent stem cell culture medium and application thereof. The composition for culturing the sheep formation state pluripotent stem cells, disclosed by the invention, contains an N2B27 basic culture medium and cell factors, wherein the cell factors contain CHIR99021, IWR1, A419259, a recovery hub IL6, a recovery hub sIL6R, a recovery hub Activin A and a recovery hub FGF2 (Fibroblast Growth Factor 2). The culture medium prepared from the composition can obtain stable sfPSCs, and can be applied to construction of sheep gene editing model animals.

The invention provides a small extracellular vesicle composite collagen hydrogel injection, and belongs to the technical field of osteoarthritis, and the small extracellular vesicle composite collagen hydrogel injection is prepared by the following steps: S1, mixing a sodium alginate solution with a type I collagen solution to obtain a mixed solution, adding a CaCl2 solution into the mixed solution to form gel, adding an FGF18 solution into the gel, and mixing to obtain FGF18 sodium alginate / collagen hydrogel; s2, transfecting a Lenti-IGF-1-EGF lentiviral vector to P3 generation human umbilical cord mesenchymal stem cells, culturing to obtain P5 generation IGF-1 overexpressed MSCs cells, namely P5 generation IGF-1-MSCs cells, co-culturing with the P5 generation IGF-1-MSCs cells by using curcumin, and separating and extracting a small extracellular vesicle solution rich in curcumin; and S3, mixing the small extracellular vesicle solution rich in curcumin with the FGF18 sodium alginate / collagen hydrogel, so as to obtain the small extracellular vesicle composite collagen hydrogel injection. The invention provides a small extracellular vesicle composite collagen hydrogel injection, which achieves the purposes of relieving inflammatory injury of osteoarthritis and enhancing the treatment effect of osteoarthritis.

The invention discloses a perfusion device for stem cell culture, and belongs to the technical field of cell culture.The perfusion device comprises a frame, a closing assembly convenient to overhaul is arranged on the frame, a culture container is arranged in the frame, a perfusion bottle is fixedly installed in the frame, a filter box is fixedly installed in the frame, and the filter box is connected with the perfusion bottle. A pumping assembly is arranged in the frame, a penetrating groove is formed in the top of the filter box, and a connecting pipe is connected to the filter box; culture cells are conveniently collected through the arranged filter cylinder, the cells cultured in the perfusion bottle and a culture solution flow into the filter box through a liquid supply pipe, the filter cylinder is covered with a filter membrane, the liquid can flow to the outside to intercept the cells, then the cells can be filtered in the filter cylinder to be collected, then the filter cylinder is rotated, and the cells can be conveniently collected. The threaded sleeve is separated from the threaded head, so that the filter cartridge can be detached, the cells can be conveniently separated and collected, and the working efficiency is improved.

The invention relates to an N2B27 culture medium for inducing generation of bidirectional pluripotent stem cells, one of LY2090314, AS1842856 or CHIR99021 is added into an N2B27 basal culture medium, and the concentration of the CHIR99021 is 10 mu M. The invention also relates to a preparation method of the N2B27 culture medium. The weight of the LY2090314 is 10 nM, and the weight of the AS1842856 is 0.6 [mu] M. The invention also provides an application of the culture medium in induced production of bidirectional pluripotent stem cells. According to the invention, a novel stem cell culture condition for promoting co-expression of genes OCT4 and CDX2 by adding small molecules LY2090314 (LY), AS1842856 (AS) and CHIR99021 to inhibit signal channels of FOXO1 and GSK3 is screened out. The culture conditions are simple and convenient, the application range is wide, the cultured cells keep the characteristics of 16-32 cell stages of the embryos, and the development characteristics of the embryos can be efficiently reproduced. Under the condition of not depending on transcription factors, the screened bidirectional pluripotent stem cells (BPSCs) can promote efficient generation of TSC cells and establish a TSC cell line through independent differentiation of a serum culture medium or induced differentiation of a TSC culture medium. The obstacles between early embryo pedigree are broken, and the research on early embryo development is promoted.

The invention discloses a cell fate distinguishing and differentiation prediction method based on a three-dimensional form, and belongs to the technical field of cell fate distinguishing and differentiation prediction. In order to solve the problem that cell fate differentiation is difficult to distinguish and predict under a small sample size, the method comprises the following steps: acquiring a living biological sample, and imaging by using a three-dimensional delay microscope to obtain cell three-dimensional image data; segmenting the cell membrane boundary and extracting geometric and high-order three-dimensional morphological characteristic parameters; labeling cell fate and differentiation states based on means such as cell lineage tracking or gene fluorescence labeling; and finally, determining an optimal morphological index and a minimum sample size through single-side double-sample t test, and applying the optimal morphological index and the minimum sample size to two new cell populations and outputting a cell fate distinguishing and differentiation prediction result. The method can realize cross-species, low-loss, low-cost and high-precision cell fate differentiation and differentiation prediction, and is suitable for development research, stem cell culture and disease diagnosis.

The invention relates to the technical field of rhinitis spray preparation, in particular to a method for preparing allergic rhinitis spray from umbilical cord mesenchymal stem cell exosomes, which comprises the following steps: 1, umbilical cord treatment; 2, culturing umbilical cord mesenchymal stem cells; 3, preparing an umbilical cord mesenchymal stem cell exosome; and 4, preparing the rhinitis spray. The rhinitis spray prepared by the method disclosed by the invention is simpler and more convenient to use. The exosome, the platelet growth factor and other substances in the rhinitis spray well solve the core problems of single curative effect, large side effect, low compliance and the like in the traditional rhinitis treatment, and through the induction and repair functions of the stem cell exosome and the bactericidal ability of the antibacterial peptide, the nasal cavity environment is improved, the anaphylactic reaction is reduced, the nasal mucosa is repaired, and the rhinitis treatment effect is improved. The allergic rhinitis is expected to be fundamentally treated.

The invention provides an extraction method of umbilical cord mesenchymal stem cell exosomes. The extraction method comprises the following steps: S1, collecting umbilical cord mesenchymal stem cell culture supernatant; s2, centrifuging the culture supernatant under a centrifugal force of 250-350 g, and collecting the supernatant to obtain a first mixed solution; s3, centrifuging the first mixed solution under a centrifugal force of 1500-2500 g, and collecting a supernatant to obtain a second mixed solution; s4, centrifuging the second mixed solution under a centrifugal force of 9000-11000 g, and collecting a supernatant to obtain a third mixed solution; and S5, filtering the third mixed solution, centrifuging under the centrifugal force of 90000-110000 g, collecting the precipitate, adding a PBS buffer solution, centrifuging under the centrifugal force of 90000-110000 g, and collecting the precipitate to obtain the umbilical cord mesenchymal stem cell exosome. The umbilical cord mesenchymal stem cell exosome with the purity up to 99% is extracted from the culture supernatant through twice low-speed centrifugation and three times of high-speed centrifugation.

The present application relates to the technical field of biological medicine, and particularly relates to a stem cell culture method for promoting wound healing and application thereof. The stem cell culture method for promoting wound healing comprises the following steps: step one, providing umbilical cord-derived mesenchymal stem cells; step two, adding luteolin and baicalin as culture medium additives in a serum-supplemented culture medium, wherein the concentration of luteolin is 1-10 micromoles / L, and the concentration of baicalin is 1-10 micromoles / L; step three, inoculating the mesenchymal stem cells into the culture medium containing the additives for culture, and the culture condition is 34-40 DEG C, 4-6% CO2, and the culture time is 48-96 hours; step four, harvesting the cultured mesenchymal stem cells to obtain stem cells for promoting wound healing. The culture method can promote the stem cells to secrete various growth factors, significantly improve the secretion of PGE2 and VEGF-C growth factors, and make the wound healing rate of the stem cell treatment group reach 96%, which has a significant effect on treating diabetic foot ulcers.

The invention relates to the technical field of bioengineering and digital twinning, and discloses a digital twinning modeling regulation and control system for a stem cell culture process, which comprises a signal acquisition module, a space-time coding module, a dynamic modeling module, a regulation and control decision module, a closed-loop execution module and an interactive interface module, according to the method, multi-modal data is collected through a composite sensor and coded into a space-time tensor, a gene noise-metabolic force field cross-scale dynamic model is constructed, a microenvironment regulation and control instruction is generated in combination with a multi-agent game, closed-loop cooperative regulation and control of a biochemical gradient field and mechanical parameters are achieved, and dynamic interactive calibration is achieved through a three-dimensional thermodynamic diagram. According to the method, a stem cell differentiation cross-scale dynamic model is constructed through multi-modal data fusion, microenvironment closed-loop regulation and control are realized by using space-time tensor coding and a multi-agent game strategy, and prediction precision and regulation and control real-time performance of a digital twin system are improved in combination with a three-dimensional visual interaction platform.

The invention relates to the technical field of data processing of stem cell culture equipment, and discloses a data processing system of stem cell culture equipment, which comprises the following modules: a data acquisition module generates a state and a control sequence by acquiring parameters in a stem cell culture process; the state modeling module constructs a multi-dimensional state matrix and a control matrix based on the collected data; the trend prediction module is used for constructing a state transition model and generating a prediction state vector at the next moment; the optimization control module solves a control strategy vector based on the prediction state vector; the control execution module is used for converting the control strategy vector into an actual control instruction; the closed-loop feedback module collects the actual state after adjustment and is used for correcting the state and the control matrix and dynamically updating the state transition model. According to the method, the state matrix and the control matrix based on the sliding time window are constructed, so that multi-dimensional time sequence information in the stem cell culture process is subjected to structured expression, and the effect of capturing the dynamic change trend of the environment in real time is achieved.

The invention provides a serum-free stem cell culture medium and a preparation method thereof, and the culture medium realizes long-term stable culture of stem cells under a completely serum-free condition by optimizing basic culture medium components and adding a specific growth factor combination, an extracellular matrix simulant and a metabolism regulator. The culture medium comprises a basic culture medium, a recombinant human protein substitute, a cell adhesion promoting factor, a growth factor combination, an antioxidant and a metabolism regulator. Compared with the prior art, the invention has the following advantages: 1) animal-derived components are completely avoided, and the immunogenicity and the difference between batches are reduced; 2) maintaining the dryness of the stem cells through a specific growth factor combination; 3) adding a metabolism regulator to optimize cell energy metabolism; 4) the cost is obviously lower than that of commercially available like products; and 5) supporting long-term culture of stem cells without spontaneous differentiation. The invention also provides a preparation method of the culture medium and a method for culturing stem cells by using the culture medium.

The invention discloses a mechanical response type engineered mesenchymal stem cell exosome preparation for treating diseases caused by fibrosis inflammation, a preparation method and application, and the exosome preparation is an exosome which is secreted by mesenchymal stem cells and has high cholesterol and high-efficacy protein. The exosome is prepared by culturing mesenchymal stem cells on a hydrogel matrix in a modulus hardness range of 1-50KPa, the exosome secretion amount of a single cell is (3-5) * 10 < 4 > / cell, the cholesterol content is (1-10) nM / 5mg exosome, and the size of the exosome is 110-140nm; the high-efficacy protein is a key functional protein for regulating and controlling the biological activity of the exosome and the cell action efficiency. The cholesterol content in the exosome is precisely regulated and controlled, the cytoplasm delivery efficiency and tissue penetrability of the exosome are improved, a whole-process control system of hydrogel preparation, stem cell culture and exosome purification is constructed, and the stability of the preparation and the yield of the exosome are improved.

The invention relates to the technical field of cell culture, and discloses a stem cell differentiation degree identification method based on image features. The method comprises the following steps: continuously capturing multi-temporal image data in a stem cell culture process through a high-resolution microscopic imaging system, and generating an image and environment synchronous data set; processing the data set, extracting dynamic morphological features and texture change modes of the stem cells, and constructing a fusion feature set; outputting a differentiation process index based on the fusion feature set; a differentiation degree grade division rule is set, and a differentiation process index and differentiation grade mapping table is established; in a real-time application stage, acquiring real-time imaging data and environment readings, and inputting the real-time imaging data and the environment readings into an evaluation network to calculate real-time differentiation process indexes; querying the mapping table according to the real-time index to obtain a differentiation grade judgment result; comparing the judgment result with a preset target range, and if the judgment result falls into the target range, automatically executing differentiation state marking and culture parameter adjusting operation.

The invention provides a stem cell culture process information abnormity early warning method, which comprises the following steps: preprocessing original data in a stem cell culture process, extracting layered features to obtain basic statistical features, time sequence trend features and environmental factor interaction features, and updating the features in real time through a dynamic sliding window, after the verified feature set is obtained, fusion calculation is carried out to obtain a multi-dimensional fusion feature set; calculating a trend deviation degree, a feature correlation degree and a fluctuation dispersion degree of the multi-dimensional fusion feature set, and carrying out dynamic weight distribution and fusion to obtain a fusion anomaly degree value; and matching the fusion anomaly degree value with a multi-level threshold value to obtain a matching result, judging an abnormal state type in combination with an association relationship between the feature anomaly type and the cultivation stage, and generating an anomaly early warning result. According to the method, through data preprocessing, layered feature extraction and fusion and dynamic anomaly calculation, the accuracy, real-time performance and practicability of stem cell culture anomaly early warning are improved.

The invention discloses a culture method for hypoxia culture of umbilical cord mesenchymal stem cells, which comprises the following steps: hypoxia pre-adaptive culture: inoculating umbilical cord mesenchymal stem cells in a culture medium containing bFGF (basic fibroblast growth factor), glutathione, astragalus polysaccharide and salidroside, and transferring the umbilical cord mesenchymal stem cells into a 5% O2 environment after the umbilical cord mesenchymal stem cells are preliminarily adhered to the wall in a normal oxygen environment; performing low-oxygen enrichment culture: replacing a second culture medium containing astragalus polysaccharide, salidroside, D-ribose, sodium pyruvate and D-glucose, and promoting efficient cell proliferation under 2% O2; performing function strengthening culture: adding tanshinone IIA and GSK-269962A, inducing cells to secrete VEGF under 1% O2, and maintaining high dryness. According to the method, through the synergistic effect of the three-stage gradient oxygen concentration and the specific serum-free culture medium, the cell proliferation multiple is remarkably increased, the total apoptosis rate is reduced, VEGF secretion is promoted, the multidirectional differentiation potential is reserved, the culture method is standardized in operation, the serum-free culture medium is definite in component, and a high-quality stem cell culture scheme is provided for the field of regenerative medicine.

The invention discloses a bionic bone marrow microenvironment assembly for hematopoietic stem cell in-vitro amplification and a preparation method. The bone marrow microenvironment assembly comprises a three-dimensional scaffold and osteoblasts (OBs), wherein the three-dimensional scaffold comprises chitosan (CS) and sodium alginate (Alg) which are prepared into mixed gel, and the mixed gel is freeze-dried to prepare the three-dimensional scaffold; wherein the osteoblasts (OBs) are obtained by culturing and induced differentiation of umbilical cord mesenchymal stem cells (MSCs) inoculated on a three-dimensional scaffold, and the osteoblasts (OBs) as stromal cells and the three-dimensional scaffold jointly form the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly; in the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly, the preparation of the three-dimensional scaffold comprises the processes of freezing, remelting, freezing, crosslinking, freeze-drying and the like, so that a three-dimensional space form with wrinkles on the pore surface and pore size distribution between 70 microns and 400 microns is generated in the three-dimensional scaffold, and the three-dimensional space form is closer to a real stem cell niche; therefore, the practical application effect of the bionic bone marrow microenvironment assembly for in-vitro amplification of the hematopoietic stem cells is further improved.

The invention belongs to the field of stem cell culture, and relates to a pluripotent stem cell culture medium, which comprises an E6 basal culture medium, 2 ng / mL of TGF-beta1, 0-50 ng / mL of FGF-2, and 0.01-1 [mu] M of SUN11602. According to the technical scheme, proliferation and pluripotency of the hPSC are effectively maintained in a mode of combining small chemical molecules with low-concentration FGF-2.

This invention provides a method for propagating pluripotent stem cells (PSCs) by suspension culture in a bioreactor. [Solution] The method comprises the following steps: (i) adding a ROCK inhibitor (ROCKi) to pluripotent stem cells cultured in suspension in a bioreactor; (ii) adding a cell dissociation agent, thereby dissociating aggregates of pluripotent stem cells; (iii) diluting the cell dissociation agent added in step (ii) by adding a sufficient excess volume of culture medium to reduce the concentration of the cell dissociation agent to a concentration at which cell aggregates can be re-formed; and (iv) culturing the mixture obtained in step (iii) under appropriate conditions that allow for the proliferation of PSCs.

The invention relates to a cellulose-based hydrogel prepared based on a cellulose copolymer, a cellulose derivative copolymer or a blend thereof, ultramicro fibers of cellulose form a net structure, and the cellulose-based hydrogel has a highly-ordered three-dimensional micro-nano structure, is better in transparency and is more beneficial to observation of a cell state. The cellulose-based hydrogel is suitable for culture of 3D cell culture tissues and organoids, the stem cells cultured by the cellulose-based hydrogel can form cluster culture stem cells and organoids, the operation is simple, compact fused monolayer endoderm cells are formed, non-directional differentiation of the stem cells can be inhibited, and the formed organoids are thick in vesicle wall and are in a solid shape. And the obtained product is a differentiated mature organ and can be used for high-throughput drug screening, drug toxicity testing and regenerative medicine.

The invention discloses a culture medium for adipose-derived stem cells, and particularly relates to the technical field of stem cell culture. The culture medium comprises a basic culture medium and nerve-vascular niche components, wherein the basic culture medium comprises DMEM / F12, 10% of human-derived platelet lysate and 1% of antibiotic solution; the nerve-vascular niche comprises the following components: a Slit3 recombinant protein, a nerve growth factor-beta, a glial cell-derived neurotrophic factor, a lysyl oxidase inhibitor, dexamethasone, 3-isobutyl-1-methylxanthine and insulin. The traditional fetal calf serum is replaced by the human-derived platelet lysate, so that the risks of immunological rejection and pathogen pollution are avoided; through the synergistic effect of nerve-vessel niche factors, blood vessel maturation and innervation are promoted, programmed mechanical stretching culture is combined, the adipogenic differentiation efficiency of adipose-derived stem cells is remarkably improved, and engineered adipose tissue which is huge in lipid droplet, compact in structure and provided with a nerve-vessel network is formed.

Belongs to the field of cell culture media. The stem cell culture medium contains 20-80% of protein hydrolysate on the basis of the weight of the total components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high free amino acid yeast hydrolysate, a high nucleotide yeast hydrolysate, a high glutathione yeast hydrolysate, a high polypeptide type yeast hydrolysate, a rice protein hydrolysate, a high glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The animal stem cell natural culture medium is prepared by taking the full-nutrition protein hydrolysate as a core raw material, so that the nutritional requirements of animal stem cell proliferation can be met under the condition of not additionally adding or adding a small amount of amino acids, vitamins, growth factors and serum, and the cost of the stem cell culture medium is effectively reduced; therefore, the method has a wide application prospect in the field of food such as cell culture meat.

The invention relates to the technical field of stem cell culture, in particular to a method for constructing a bionic microenvironment for three-dimensional co-culture of stem cells, which comprises the following steps: creating a micro-trap array on a first culture substrate obtained in advance to obtain a second culture substrate; dropping the stem cell mixed solution into a micro-well array of a second culture substrate; covering the surface of the micro-well array with hydrogel to obtain a first culture environment, and incubating to obtain a second culture environment; the bioreactor is connected with a second culture environment through an interface device, and shear force is applied to the second culture environment through the bioreactor; adjusting the position of the interface device according to the distribution uniformity of the shearing force until the distribution uniformity of the shearing force is not lower than a uniformity threshold value or reaches a stopping condition; the position of the connector device is fixed, and shearing force is continuously applied. According to the method, the appropriate micro-trap size is determined according to autonomous behavior parameters, and meanwhile, a method for adjusting the shearing force application point is designed, so that a more uniform stem cell culture environment is obtained.

The invention discloses a plant stem cell nutrition synergist, and belongs to the technical field of plant stem cell nutrient solutions. The nutrient synergist can significantly improve the proliferation rate and survival rate of stem cells, promotes differentiation of the stem cells to target cell types, plays an important role in stem cell culture, can significantly improve the culture efficiency of the stem cells, provides comprehensive nutrient support and a suitable growth environment for stem cell culture, and has good application prospects. Proliferation and differentiation of the stem cells are promoted, and further research on the plant stem cells is facilitated.

The utility model relates to the technical field of stem cell culture, and discloses efficient stem cell sampling equipment which comprises a sampling box, the top of an assembly cavity is fixedly connected with a mounting plate, a sampling barrel is embedded in the center of the mounting plate, a rotating mechanism is arranged in the center of the sampling barrel, and the rotating mechanism is connected with the mounting plate. The top of the rotating mechanism is connected with a containing mechanism, the containing mechanism comprises a containing disc, clamping grooves, a first sampling assembly, a mounting groove and a second sampling assembly, the multiple clamping grooves are formed in the circumference of the edge of the containing disc at equal intervals, the first sampling assembly is connected into the clamping grooves in a clamped mode, and the second sampling assembly is connected into the mounting groove in a clamped mode. According to the equipment, the accommodating mechanism is arranged, the sampling assembly I and the sampling assembly II are used for stably placing the sampling tube and the sampling box on the accommodating disc, and the accommodating disc is driven by the rotating mechanism to rotate so as to realize sampling, so that the adaptability of the equipment to different sampling containers is improved, and the equipment is convenient to operate and replace parts.

The invention discloses a stem cell constant temperature device, and belongs to the technical field of stem cell storage, the stem cell constant temperature device comprises a cabinet body, the lower side in the cabinet body is provided with a partition part, the partition part comprises a fixed cylinder fixedly connected to the lower side in the cabinet body, the upper side in the fixed cylinder is provided with a material gathering cavity, and the left side and the right side in the fixed cylinder are hinged to baffles; according to the stem cell culture cabinet, the baffle is arranged to separate the fixed cylinder from the inner space of the cabinet body after stem cells and culture solution enter the deep position of the fixed cylinder, and the space for collecting the stem cells and the culture solution is separated from the inner cavity of the cabinet body by the baffle; according to the stem cell storage cabinet, the contact time of stem cells and a culture solution and air in the cabinet body is effectively shortened, the phenomenon that the storage effect of other stem cells is affected due to temperature rise of a gas phase area is avoided, meanwhile, the leaked stem cells and the culture solution can be collected in time through the material collecting cavity, and the stem cells and the culture solution are prevented from damaging temperature control equipment in the cabinet body.

The utility model discloses a portable preservation box for stem cell culture, which comprises a box body, the inside of a mounting shell is divided into circulation channels through a plurality of groups of placing boxes, and the circulation channels are used for prolonging the forced contact time of air and dry ice; the air inlet end of the air pump communicates with two sets of extraction pipes used for extracting air to flow in the circulation channel. Through the design of the mounting shell, the placement box, the air pump, the extraction pipe, the exhaust pipe and the like, and through the cooperation of the air pump, the extraction pipe and the exhaust pipe, air in the box body can quickly flow in the mounting shell, and the air is forced to be in contact with dry ice in the placement box when flowing in the mounting shell, so that the air is quickly cooled and uniformly circulated; therefore, a cold source can be utilized to the maximum extent, energy waste is reduced, a set low-temperature environment can be recovered in a short time through an active refrigeration mode, the time for exposing stem cells to non-ideal temperature is shortened, and cell activity decline or cell death is avoided.