Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Serum-free medium for umbilical cord mesenchymal stem cells

A mesenchymal stem cell and medium technology, applied in the field of umbilical cord mesenchymal stem cell medium, can solve the problems of the number of contaminated cells, no serum, and cost reduction, and achieve the effects of cost reduction, clear ingredients, and promotion of proliferation

Inactive Publication Date: 2016-03-23
SHANDONG JINGYUAN BIOTECH CO LTD
View PDF2 Cites 34 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical task of the present invention is to solve the deficiencies of the prior art, provide a serum-free umbilical cord mesenchymal stem cell culture medium, and overcome the contradiction between the defects of exogenous contamination of serum and the number of cell expansion and cost reduction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Serum-free medium for umbilical cord mesenchymal stem cells
  • Serum-free medium for umbilical cord mesenchymal stem cells
  • Serum-free medium for umbilical cord mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] A serum-free umbilical cord mesenchymal stem cell culture medium of the present invention is described in detail below.

[0042] A serum-free umbilical cord mesenchymal stem cell culture medium of the present invention, and an experimental method for using the culture medium to subculture and expand umbilical cord mesenchymal stem cells, the experiments carried out are as follows:

[0043] (1) Combination experiment of cytokines in serum-free medium:

[0044] Selected growth factors: recombinant human fibroblast growth factor 2 (hFGF), human recombinant insulin (hI), human recombinant epidermal growth factor (hEGF), human recombinant leukemia inhibitory factor (hLIF) and astragalus polysaccharide (APS) concentrate , sterilized by sterile filtration, refrigerated or frozen for later use.

[0045] Design different concentrations for different growth factors ( Table 1 ) was added to the culture medium and the optimal effect concentration of the growth factor was found a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a serum-free medium for umbilical cord mesenchymal stem cells and belongs to the technical field of cell culture. The serum-free medium for umbilical cord mesenchymal stem cells comprises a basal culture medium body and added ingredients, wherein the basal culture medium body is a DMEM culture medium; the added ingredients include a basic fibroblast growth factor hFGF, a epidermal growth factor hEGF, insulin hI, a leukaemia inhibitory factor hLIF and astragalus polysaccharide. The umbilical cord mesenchymal stem cells are subjected to subculture and amplification in the medium, and the surfaces of the cultured cells are marked and analyzed. The serum-free medium for umbilical cord mesenchymal stem cells overcomes the defect of exogenous pollution of serum and solves the problem of contradiction between the cell expansion number and cost reduction. The medium is free of serum, thereby preventing influence of animal-derived serum ingredients on cell culture. The medium can be used for studying the differentiation and proliferation adjusting mechanism of the umbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a serum-free culture medium for umbilical cord mesenchymal stem cells. Background technique [0002] Umbilical cord mesenchymal stem cells refer to a kind of pluripotent stem cells existing in neonatal umbilical cord tissue. The cell surface strongly expresses specific markers CD90, CD73, and CD105, but does not express markers CD45, CD14, and CD11 of hematopoietic or endothelial cells. , CD34, CD19, low expression or no expression of major histocompatibility antigen HLA-DR. Studies have shown that umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions such as bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. Compared with bone marrow mesenchymal stem cells, the content and proliferation ability of umbilical cord mesenchymal stem cells are superior to the latter, and have ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0775
Inventor 杨继建杜丽英田丽娜王伟徐娅妮孙镇秦倍倍
Owner SHANDONG JINGYUAN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com