Serum-free medium for umbilical cord mesenchymal stem cells

A mesenchymal stem cell and medium technology, applied in the field of umbilical cord mesenchymal stem cell medium, can solve the problems of the number of contaminated cells, no serum, and cost reduction, and achieve the effects of cost reduction, clear ingredients, and promotion of proliferation

Inactive Publication Date: 2016-03-23
SHANDONG JINGYUAN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical task of the present invention is to solve the deficiencies of the prior art, provide a serum-free umbilical cord mesenchymal stem c...

Method used

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  • Serum-free medium for umbilical cord mesenchymal stem cells
  • Serum-free medium for umbilical cord mesenchymal stem cells
  • Serum-free medium for umbilical cord mesenchymal stem cells

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Embodiment Construction

[0041] A serum-free umbilical cord mesenchymal stem cell culture medium of the present invention is described in detail below.

[0042] A serum-free umbilical cord mesenchymal stem cell culture medium of the present invention, and an experimental method for using the culture medium to subculture and expand umbilical cord mesenchymal stem cells, the experiments carried out are as follows:

[0043] (1) Combination experiment of cytokines in serum-free medium:

[0044] Selected growth factors: recombinant human fibroblast growth factor 2 (hFGF), human recombinant insulin (hI), human recombinant epidermal growth factor (hEGF), human recombinant leukemia inhibitory factor (hLIF) and astragalus polysaccharide (APS) concentrate , sterilized by sterile filtration, refrigerated or frozen for later use.

[0045] Design different concentrations for different growth factors ( Table 1 ) was added to the culture medium and the optimal effect concentration of the growth factor was found a...

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Abstract

The invention provides a serum-free medium for umbilical cord mesenchymal stem cells and belongs to the technical field of cell culture. The serum-free medium for umbilical cord mesenchymal stem cells comprises a basal culture medium body and added ingredients, wherein the basal culture medium body is a DMEM culture medium; the added ingredients include a basic fibroblast growth factor hFGF, a epidermal growth factor hEGF, insulin hI, a leukaemia inhibitory factor hLIF and astragalus polysaccharide. The umbilical cord mesenchymal stem cells are subjected to subculture and amplification in the medium, and the surfaces of the cultured cells are marked and analyzed. The serum-free medium for umbilical cord mesenchymal stem cells overcomes the defect of exogenous pollution of serum and solves the problem of contradiction between the cell expansion number and cost reduction. The medium is free of serum, thereby preventing influence of animal-derived serum ingredients on cell culture. The medium can be used for studying the differentiation and proliferation adjusting mechanism of the umbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a serum-free culture medium for umbilical cord mesenchymal stem cells. Background technique [0002] Umbilical cord mesenchymal stem cells refer to a kind of pluripotent stem cells existing in neonatal umbilical cord tissue. The cell surface strongly expresses specific markers CD90, CD73, and CD105, but does not express markers CD45, CD14, and CD11 of hematopoietic or endothelial cells. , CD34, CD19, low expression or no expression of major histocompatibility antigen HLA-DR. Studies have shown that umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions such as bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. Compared with bone marrow mesenchymal stem cells, the content and proliferation ability of umbilical cord mesenchymal stem cells are superior to the latter, and have ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 杨继建杜丽英田丽娜王伟徐娅妮孙镇秦倍倍
Owner SHANDONG JINGYUAN BIOTECH CO LTD
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