302 results about "Cell expansion" patented technology

The invention relates to the technical field of cell automation augmentation and culture instrument, in particular relates to an automation augmentation and culture system of induced pluripotent stem cells, which comprises a cell automation augmentation and culture system, an operation room and a control system which are mutually connected, wherein, the cell automation augmentation and culture system comprises a culture box and a culture box control room connected with the culture box; the culture box comprises at least one culture device for fixing a culture container fixed block of a culture container, a culture container cover automatic opening and closing system, a culture container automatic popping and closing system, a digital temperature system and at least one sensor; the culture box control room comprises an air flow purification and induction system and a culture box digital power system; and the operation room comprises a bar code automatic entry system, an liquid automatic replace system and a cell on-line observing system. By utilizing the system provided by the invention, a somatic cell can be automatically induced into a pluripotent stem cell, and the system can be used for stem cell research and clinical stem cell treatment.

The present application describes various applications of the non-expansion protocol for the preparation of an injectable autologous cell mixture of the present invention that can be used to prevent symptoms in a number of indications. Cells are isolated from surgically derived tissue and are at least partially disaggregated from each other. The heterologous cell mixture is mixed with growth factors, differentiation agents, extracellular matrix proteins and / or microspheres and injected into the patient without cell expansion. The harvesting of tissue, cell isolation, and injection are performed within a single surgical procedure lasting only minutes to hours.

The invention provides an automated apparatus of cell culture having tanks of culture medium, of growth factors and of cells to be cultured, an incubator having a thermostated enclosure which houses a cell culture vessel, and control computer system. A supporting and agitation device of the culture vessel is provided in the enclosure, and the culture vessel is formed by a bag having at least one inlet port connected to the tanks and one outlet port connected devices for harvesting and storage of the cells after culture, these harvesting and storage devices and tanks being located outside the enclosure and being connected to the cell expansion bag ports by conduits which together with the cell expansion bag form a preassembled module passing through a wall of the enclosure.

A method of distributing a plurality of cells in a bioreactor of a cell expansion system includes manipulating an orientation of the bioreactor such that a net impulse due to gravity acting on the plurality cells in the bioreactor is reduced. One method includes (a) rotating the bioreactor at an angular velocity ω about an axis of rotation and through an angular displacement θ, the bioreactor rotating from a first orientation to a second orientation; (b) holding the bioreactor still at the second orientation for a first period of time t1, wherein t1 substantially equals 2 / ω; (c) rotating the bioreactor at the angular velocity ω about the axis of rotation and through the angular displacement θ; and (d) holding the bioreactor still for a second period of time t2, wherein t2 substantially equals t1.

A method of cell expansion is provided. The method comprising culturing adherent cells from placenta or adipose tissue under three-dimensional culturing conditions, which support cell expansion.

The present invention relates to a method for cell expansion. More closely, it relates to a method for expansion of cells, such as mesenchymal stem cells, on microcarriers in a plastic bag bioreactor. The invention enables expansion to therapeutic amounts of stem cells. The method comprises the following steps: a) addition of cells in cell culture medium and microcarriers to a plastic bag container; b) allowing the cells to adhere to the microcarriers while the container is kept substantially still; c) addition of further cell culture medium once the cells have adhered; d) culturing the cells under gentle and constant agitation; e) increase the surface area for continued culturing; and f) final harvesting of cells by an active detachment and separation step.

The invention provides a serum-free medium for umbilical cord mesenchymal stem cells and belongs to the technical field of cell culture. The serum-free medium for umbilical cord mesenchymal stem cells comprises a basal culture medium body and added ingredients, wherein the basal culture medium body is a DMEM culture medium; the added ingredients include a basic fibroblast growth factor hFGF, a epidermal growth factor hEGF, insulin hI, a leukaemia inhibitory factor hLIF and astragalus polysaccharide. The umbilical cord mesenchymal stem cells are subjected to subculture and amplification in the medium, and the surfaces of the cultured cells are marked and analyzed. The serum-free medium for umbilical cord mesenchymal stem cells overcomes the defect of exogenous pollution of serum and solves the problem of contradiction between the cell expansion number and cost reduction. The medium is free of serum, thereby preventing influence of animal-derived serum ingredients on cell culture. The medium can be used for studying the differentiation and proliferation adjusting mechanism of the umbilical cord mesenchymal stem cells.

The present invention relates to methods for the induction and a cell to enter the Islet 1+ (Isl1+) lineage and methods for expansion of cells of islet 1+ lineage. One aspect of the present invention relates to methods to induce a cell to enter the islet 1+ lineage, and more particularly to a method to induce a cell to enter a the Isl1+ lineage to become an Isl1+ progenitor that is capable of differentiating along multiple different lineages such as a endothelial lineage, a smooth muscle lineage or a cardiac lineage. In particular, one embodiment present invention relates to methods to induce a cell to enter the Isl1+ lineage by inhibiting a wnt signalling pathway in the cell. Another aspect of the present invention relates to methods to expand a cell of the Isl1+ lineage, such as a Isl1+ progenitor by activating a wnt signalling pathway in the Isl1+ progenitor. Another aspect of the present invention relates to use of cells of the isl1+ lineage in subjects for therapeutic and preventative treatment of cardiovascular diseases.

Methods for treating a subject suffering from a compromised endogenous hematopoietic system are described that comprise administering to the subject a therapeutically effective amount of adherent stromal cells. Methods of preparing adherent stromal cells and pharmaceutical compositions comprising the cells are also described.

The invention discloses a network expansion method and device. The method comprises the steps of in a pre-stored first correspondence relationship between a first user perception rate threshold and a first network expansion index threshold, obtaining a second correspondence relationship of a region to be evaluated between a second user perception rate threshold and a second network expansion index threshold; determining a current user perception rate and a current network expansion index of the region to be evaluated; comparing the current user perception rate and the current network expansion index with the second user perception rate threshold and the second network expansion index threshold in the second correspondence relationship; and carrying out carrier frequency expansion for cell to be expanded when the obtained comparison result conforms to a cell expansion condition. The invention is used for solving the problem that generally, an LTE network mode is based on the standard mainly from corresponding threshold requirements given by the LTE network load and network planning and design requirements and the limitation is high.

Methods for treating a subject suffering from a compromised endogenous hematopoietic system are described that comprise administering to the subject a therapeutically effective amount of adherent stromal cells. Methods of preparing adherent stromal cells and pharmaceutical compositions comprising the cells are also described.

The present invention relates generally to immunotherapy. Disclosed herein are methods for obtaining cytolytic differentiated NKG2A−NKG2C+ cells with a given KIR specificity and also compositions comprising these cells as well as the use of these cells for therapy. The NK cell expansion methods provided herein also have non-therapeutic uses.

The invention discloses a dual-channel POTN linear protection system, method and device, and relates to the field of a POTN device. The device comprises a dual-channel establishing module, a cell expansion head adding module, a grouping cell determination module, an OTN service protection module and a PTN service protection module. The dual-channel establishing module is connected with the grouping cell determination module through the cell expansion head adding module; and the grouping cell determination module is connected with the OTN service protection module and the PTN service protection module. The dual-channel POTN linear protection system, method and device can be suitable for the high-capacity OTN service, and meanwhile, can realize various protections on PTN services conveniently and flexibly, are not only wide in application range, but also convenient for people to use.

The invention relates to the technical field of cell culture and especially relates to an automatic cell expansion system. The automatic cell expansion system realizes automatic real-time regulation and control of the amount of gas dissolved in a cell growth environment and provides a stable growth environment for cell culture. The system comprises a culture liquid delivery device and a hollow fiber reactor communicated with the culture liquid delivery device through a culture liquid circulation conduit. The hollow fiber reactor comprises a culture liquid channel and a cell growth chamber. The culture liquid delivery device comprises a gas dissolver. The gas dissolver comprises a housing, a gas inlet, a culture liquid inlet and a culture liquid outlet, wherein the gas inlet, the culture liquid inlet and the culture liquid outlet are arranged on the housing. Through the culture liquid inlet and the culture liquid outlet, the gas dissolver and the culture liquid channel form a circulation loop. The automatic cell expansion system also comprises a gas supplying device communicated with the gas inlet. A gas flow control valve is arranged between the gas supplying device and the gas inlet. The automatic cell expansion system also comprises a control system electrically connected to the gas flow control valve.

The invention provides a method for in-vitro expansion of CD8<+>T cells. The method comprises steps as follows: TIL (tumor infiltrating lymphocyte) cells are extracted from a malignant pleural and peritoneal effusion source, cytokines IL-2, CD3McAb and PD-1 monoclonal antibodies are added to a TIL cell culture medium, the concentration of the IL-2 is 300-1,000 U / ml, the concentration of the CD3McAb is 10-50 ng / ml, and the concentration of the PD-1 monoclonal antibodies is 50-200 ng / ml. The cell factor combination adopted by the method can increase the TIL cell expansion multiple, effectively inhibit the negative regulation effect of Treg cells on the CD8<+>T cells and promote the anti-tumor effect.

A method and substance for regulating expansion of a stem cell, such as a hematopoietic stem cell, is provided. Therefore, a method is provided for regulating expansion of a stem cell, such as a hematopoietic stem cell, a germ line stem cell, or a neural stem cell, comprising the steps of (A) providing, to the stem cell, Bmi-1 or a variant or fragment thereof and / or a Bmi-1 regulating agent in an amount sufficient for regulation of the expansion of the stem cell and (B) culturing the stem cell for a time sufficient for the regulation of expansion.

The present invention provides methods and devices for screening a single cell or a small group of cells for a desired biological activity. In particular, the present invention provides for delivering cell(s) to a plurality of cell isolation regions of a cell isolation device, transferring cell(s) to a plurality of wells of a cell expansion device and then detecting the potential desired biological activity of the cell(s). Each of the receptacles comprise a recess sized to isolate a single cell or small group of cells and each of the wells encompass a cavity that provides sufficient volume for cell proliferation.

The invention belongs to the field of cell engineering, in particular to a method for high-efficient expansion and cryopreservation of NK (Natural Killer) cells and an application of the method. According to the invention, fusion genes are subjected to gene recombination to an AAVS1 (Adeno-Associated Virus Integration Site 1) transposon through a plasmid, and the freezing / thawing step is introduced to the NK cell expansion step taking a modified K562 cell line as a basis in the intermediate stage of cell expansion; finally, the NK cells are expanded and saved. A K562-mbIL15-41BBL-mbIL21 cell line developed by the integration technology of site specific genes is used, and the activity, purity and cytotoxicity of the NK cells are high to realize high expansion efficiency of the NK cells to improve NK expansion. The method for the high-efficient expansion and cryopreservation of the NK (Natural Killer) cells and the application of the method for the high-efficient expansion and cryopreservation of the NK (Natural Killer) cells disclosed by the invention have great market prospects and economic values.

The invention discloses a method for storing endometrial stem cells, which comprises the following steps: 1) preparing a collection tool set and preparing a culture medium; 2) collecting menses; 3) performing a sterility test; and 4) subjecting endometrial stem cell to separation culture, namely, filtering mixed liquid, which is obtained by the step 2), in a collection tube 1, collecting single karyoplasts by using a density gradient centrifugation method, inoculating the obtained single karyoplasts in a Chang complete culture medium at an inoculation density of single karyoplasts of 1*10<5> to 1*10<6> / ml, and culturing in a culture tank which is at 37 DEG C and saturated humidity and contains CO2 at a volume percentage concentration of 5 percent; 5) amplifying and purifying cells; and 6) storing cells by freezing. When the method of the invention is used, a large number of high-purity target cells, namely endometrial stem cells, can be obtained.

The invention relates to a simple and effective method of automatically extracting immune cells in APB (Adult Peripheral Blood), in particular to a method of using an AXP full-automatic cell separation equipment to extract the immune cells in the APV, a supporting freezing-thawing method, and an immune cell expansion method after the thawing. The method comprises the steps of whole blood sample preprocessing, automatically separating, mononuclear cell collection and at least one of the following items of detecting the immune cells: total mononuclear cells, mononuclear cell viability, cell contamination, genetic disease, HLA-ABC / DR matching. According to the method provided by the invention, the immune cells can be effectively obtained by using the specific operating steps, such as extraction, freezing, thawing and amplification, and the immune cells can be used for treating tumors.

The invention discloses a kit for mesenchymal stem cell culture and its application. The kit provided by the invention includes a cell culture solution A and a cell culture solution B. The cell culture solution A is a Knockout-DMEM culture medium containing 80-120ng / ml cytokine LIF and 80-120ng / ml cytokine bFGF. The cell culture solution B is fetal bovine serum. The kit provided by the invention can separate umbilical cord mesenchymal stem cells from an in-vitro umbilical cord and culture the umbilical cord mesenchymal stem cells, and has the advantages of rapid cell expansion speed and high expansion efficiency. After multiple subculturing, cell totipotency can still be maintained. In the invention, the problems of umbilical cord mesenchymal stem cell separation purity, quantity and primary culture time are solved, and a lot of umbilical cord mesenchymal stem cells can be obtained. Thus, the kit provided in the invention provides abundant sources for making use of umbilical cord mesenchymal stem cells to conduct further experimental research.

The invention provides a natural killer cell culture substrate and an amplification culture method for natural killer cells, and relates to the technical field of cell culture. The natural killer cell culture substrate is mainly composed of a serum-free culture medium, autologous plasma and cytokines; according to the amplification culture method for the natural killer cells with the natural killer cell culture substrate, the IL-2, IL-12, IL-15, IL-21 cytokines in the cell culture substrate are combined through Lymactin-NK antibodies to induce mononuclear cells of human peripheral blood to release dangerous signals, the natural killer cells are activated, and the killing activity of the cells is enhanced. According to the method, a large quantity of high-quality natural killer cells can be obtained in a short period through efficient amplification, and the problems are effectively solved that in existing culture methods for the natural killer cells, the production process is complex, the production cost is relatively high, and natural killer cells are low in amplification multiple, not high in purity and difficult to produce in a large-scale mode.

The invention provides a battery sensing system comprising a battery module comprising a plurality of battery cells, at least one sensor coil coupled to or placed adjacent to one of more of the plurality of battery cells to determine cell expansion during cell operation, and a battery management system comprising one or more processors and / or microcontrollers that control operation of the plurality of battery cells.

The invention relates to a culture medium and the usage thereof, in particular to the Chinese hamster ovary cell enlarging culture medium and the usage thereof. The invention belongs to the biological product field. The Chinese hamster ovary cell enlarging non-protein culture medium of the invention, wherein comprises minimal medium, and also comprises microelement, Transferrin and Insulin subsistent. A serum-containing routine culture medium can be directly replaced with the serum free culture medium of the Chinese hamster ovary cells, and the Chinese hamster ovary cells are not required to adapt process.

The invention relates to a tumor tissue tumor infiltrating lymphocyte (TIL) cell preparation method and a dedicated culture medium. The method comprises the following steps of tumor surrounding tissue obtaining, cell digestion, cell primary culture, cell subculture and cell collection, wherein a primary culture medium is based on a RPMI 1640 culture medium and prepared from the following concentration ingredients of 10% volume of human-derived serum, 20 to 45ng / ml of basic fibroblast growth factor (bFGF), 1 to 5mg / ml of riboflavin, 70 to 90ng / ml of cortisol, 10 to 25mg / ml of sodium dihydrogen phosphate monohydrate, 47 to 62ng / ml of recombinant human leukaemia inhibitory factor (LIF) and 500 to 800U / ml of IL-2; a subculture medium is based on the RPMI 1640 culture medium and prepared from the following concentration ingredients of 10% volume of the human-derived serum, 20 to 40mmol / L of HEPES, 1000 to 2000U / ml of the IL-2, 0.03 to 0.07mmol / L of beta-mercaptoethanol and 5 to 15ng / ml of sodium phosphate. According to the preparation method, an existing culture medium is improved, different culture mediums are utilized to culture the TIL cells in pertinence, TIL cell expansion capacity is improved, meanwhile a culture period is reduced, a culture complexity degree is reduced, a use amount of the IL-2 is reduced, and toxic reaction is reduced.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

Proliferating pancreatic islet cells obtained by the method of isolating a population of cells that preferably includes predominantly islet precursor cells that express one or more marker associated with an islet precursor cell and providing the precursor cells with one or more a pancreatic differentiation agent so that a population of cells is obtained that has a high proportion of cells with phenotypic characteristics of functional pancreatic islet β-cells. Optionally, the precursor cells are pretreated by providing them with one or more cell expansion agent to increase the number of cells in the population prior to differentiation. The pancreatic differentiation agent composition comprises a gastrin / CCK receptor ligand, e.g., a gastrin, in an amount sufficient to effect differentiation of pancreatic islet precursor cells to mature insulin-secreting cells. The cell expansion agent composition comprises one or more epidermal growth factor (EGF) receptor ligand in an amount sufficient to stimulate proliferation of the precursor cells. The methods of treatment include transplanting either undifferentiated precursor cells and providing the pancreatic differentiation agent either alone or in combination with the cell expansion agent in situ, or transplanting the functional pancreatic islet β-cells into the patient. The pancreatic islet β-cells can be used for drug screening, and replenishing pancreatic function in the context of clinical treatment.

The invention relates to a multifunctional bioreactor for cell sorting and cell culture in vitro. Said bioreactor comprises five main elements, including an adjustable magnetic field, a multifunctional cell supporting system, a protective perfusion system and a computerized control system. Said methods are for the application of the said bioreactor. The said bioreactor has the functions of cell expansion, cell directed differentiation and cell separation (sorting). It allows its all functions carried out in one reaction chamber.

The invention discloses a clinical-grade serum-free medium for adherent culture of human neural stem cells. The medium disclosed by the invention comprises a basic medium, basic nutrition additives, plant-based human serum albumins, saccharides, lipid, hormones, antioxidants and related substances for promoting metabolism; the basic medium is prepared from commercial DMEM / F12 and a commercial neurobasal medium according to a ratio of 1:1; the basic nutrition additives comprise insulin, holo-transferrin, apo-transferrin, putrescine, progesterone and sodium selenite; the hormones comprise biotin, corticosterone, lipoic acid, Ve and Ve acetic ester; the antioxidants comprise human-derived catalase, human-derived superoxide dismutase, glutathione and Vc; the related substances for promoting metabolism comprise carnitine, T3 and ethanol amine. The medium can improve a cell expansion speed by two to three times, well keeps stem properties of the neural stem cells, keeps the cell differentiation potential of the neural stem cells and eliminates potential animal-origin endotoxin and viral pollution.