114 results about "Cell expansion" patented technology

The invention discloses a cord blood-derived regulatory T cell amplification culture medium and a use method thereof, and relates to the field of animal cell culture and immune cell therapy, the culture medium is a serum-free culture medium, the culture medium is composed of a basic culture medium, a serum substitute combination, a combination of four cell factors, an immunosuppressor, an apparent stabilizer and an antioxidant, and all the components play a role synergistically; according to the use method, cell amplification is realized through a collaborative process of staged environmental regulation and targeted activation. According to the method, the problems of exogenous pollution and batch difference caused by dependence of a serum-containing system on cord blood-derived regulatory T cell amplification in the prior art are solved, the defect that a serum-free scheme is difficult to consider the amplification efficiency and the function stability at the same time is overcome, the cell amplification quality and the clinical application safety are guaranteed, and the method is suitable for large-scale clinical transformation requirements.

The invention relates to the technical field of biology, in particular to a Treg cell amplification culture medium and a preparation method of Treg cells. According to the method for in-vitro amplification of the regulatory T cells (Treg) from peripheral blood, the amplification efficiency and purity of the Treg cells are improved, the culture cost is reduced, the culture period is shortened, and the problem of trophoblast cell residues in subsequent clinical application is solved.

A cell module for an electrical energy storage device of a motor vehicle includes at least one cell structure made up of energy storage cells, a metallic cell module frame, enclosing the at least one cell structure, for clamping the energy storage cells, and a measuring device for detecting a deformation of the cell module frame related to cell expansion. The measuring device includes a passive resistive extension sensor having a deformation-dependent electrical resistance and a measuring unit connected to the extension sensor to output an electrical measurement signal characterizing the deformation-dependent electrical resistance of the extension sensor. The extension sensor is formed by the metallic cell module frame, with which the measuring unit is electrically contacted.

Thermal barrier systems are provided for inhibiting the transfer of thermal energy inside a traction battery pack. An exemplary thermal barrier system may include one or more thermal barrier assemblies arranged between adjacent battery cell groups of a cell stack. Each thermal barrier assembly may include a mica core, a first ceramic foam layer, a second ceramic foam layer, a first glass silicone layer, and a second glass silicone layer. In some implementations, the thermal barrier system may additionally include one or more cell expansion pad assemblies and / or one or more thermal barriers.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

The invention discloses a gamma delta T cell efficient amplification method and application, and belongs to the technical field of cell biology. The amplification method comprises the following steps: separating PBMC (peripheral blood mononuclear cells) by adopting a density gradient centrifugation method, carrying out initial culture through a polylysine coated container, carrying out three-stage dynamic stimulation, combining with an X-VIVO 15 culture medium of 5-8% autoserum, and finally carrying out anti-gamma delta TCR magnetic bead separation to obtain high-purity cells. Through collaborative optimization of stepped factor combination, staged container coating and a low-serum system, the amplification multiple of the gamma delta T cells reaches 150-180 times, the purity is larger than or equal to 90% after purification, the cytotoxicity and the survival ability are remarkably improved, the gamma delta T cells can be efficiently used for immunotherapy of tumors and infectious diseases, and stable technical support is provided for clinical transformation of the gamma delta T cells.

The invention discloses a method for culturing NK (Natural Killer) cells. The method comprises the following steps: inoculating a mononuclear cell into a T25 cell culture flask, wherein the T25 cell culture flask is coated by a coating factor in advance; carrying out culture treatment on the inoculated mononuclear cells in a culture system containing an NK cell culture medium containing an activation amplification factor and plasma so as to obtain NK cells; wherein the coating factor, the activation amplification factor and the NK cell culture medium are derived from an NK cell amplification kit. According to the method provided by the embodiment of the invention, the dosage of the amplification reagent can be reduced, the amplification multiple of the NK cells is improved, and the production cost is reduced.

The lithium battery for the unmanned aerial vehicle comprises a shell and at least two battery cells arranged in the shell side by side, a partition plate is arranged between every two adjacent battery cells, an avoiding hole for an expansion reserved space of the battery cells is formed in the middle of each partition plate, a positive pole piece and a negative pole piece are arranged on each battery cell, and the positive pole pieces and the negative pole pieces are arranged on the shell. The shell is provided with a positive pole hole for the positive pole piece to penetrate out, the shell is provided with a negative pole hole for the negative pole piece to penetrate out, a first welding disc for welding the positive pole piece and the negative pole piece is arranged between the negative pole hole for the negative pole piece of the previous battery cell to penetrate out and the positive pole hole for the positive pole piece of the next battery cell to penetrate out, and the first welding disc is connected to the shell. And the avoiding hole formed in the middle of the partition plate provides a reserved space required by expansion for the battery cell, so that the problem of overall deformation or damage of the battery caused by expansion of the battery cell is avoided, and the safety and the service life of the battery are favorably improved. The positive and negative plates between the adjacent battery cells are connected by introducing the first welding disc, so that the welding process is simplified.

The invention relates to a polylactic acid microcarrier and a method for cell amplification by using the polylactic acid microcarrier. The preparation method disclosed by the invention is simple and convenient to operate, the prepared polylactic acid microcarrier is uniform in particle size and pore size distribution, the microcarrier is subjected to surface retreatment, more active sites are provided for cell adhesion, a three-dimensional matrix environment closer to a body is provided, cell amplification is facilitated, the dryness and differentiation potential of stem cells are maintained, and the preparation method is suitable for large-scale popularization and application. And the microcarrier can be repeatedly used, so that the use cost is reduced.

The invention provides a cell strain for producing engineered viroid particles, a method thereof and the engineered viroid particles, the cell strain is named as SZCV-Con28 and preserved in Guangdong Microbial Culture Collection Center on October 15, 2025, the preservation number is GDMCC No: 67101, and the preservation number is GDMCC No: 67101. A plurality of corresponding cell factories for producing the engineered viroid particles are established by cell lines obtained by introducing different target gRNA elements into the cell strain, so that the corresponding engineered viroid particles can be continuously produced in batches. According to the invention, large-scale preparation can be realized through cell expanding culture, the capacity limitation is overcome, the production process flow is simplified, and the consumption of production raw materials is reduced.

An engineered trophoblast that expresses a cell surface molecule that specifically recognizes NKp46, the cell surface molecule comprising at least an antibody targeting NKp46 and a transmembrane domain. The invention discloses a cell culture medium and a method for amplifying NK cells. Compared with unengineered trophoblasts, the engineered trophoblasts are used for activating NK cells, and the amplification capacity of the NK cells and the killing activity of tumor cells can be enhanced.

This invention discloses a flexible battery module thermoforming bracket and protection method with adaptive cell expansion, belonging to the field of thermoforming bracket technology. It includes a thermoforming bracket body, cell positioning cavities, an adaptive mechanism, an adjustment mechanism, a detection and movement mechanism, and a buffer mechanism. Sixteen cell positioning cavities are located at the top center of the thermoforming bracket body. The adaptive mechanism uses springs, electrical springs, resistance strain gauges, telescopic rods, and spherical blocks to actively and adaptively buffer cell expansion, preventing permanent failure of the springs and monitoring the expansion force to avoid cracking and deformation of the thermoforming bracket body. The adjustment mechanism dynamically adjusts the internal dimensions of the cell positioning cavities according to the cell expansion state by inflating and deflating the airbags, actively adapting to cell expansion and contraction, preventing cracking and deformation of the thermoforming bracket body, maintaining pre-tight positioning of the cells, preventing short circuits, and improving the structural protection of the thermoforming bracket body.

Chimeric antigen receptors (CARs) with binding domains derived from a novel suite of human CD33-binding antibodies are described. The CARs include optimized short and intermediate spacer regions. The current disclosure also provides methods of cell expansion / activation processes utilizing IL-2, IL-7, IL-15, and / or IL-21 that improve cellular proliferation and cell lysis of the CARs as described.

The invention relates to the technical field of gene engineering, in particular to a ZmPIF4 gene with heat resistance and application of the ZmPIF4 gene, the nucleotide sequence of the ZmPIF4 gene is shown as SEQ ID NO.1, the ZmPIF4 gene is mainly located in a cell nucleus and possibly plays an important role in the cell nucleus signal transduction process, and under high temperature stress, the ZmPIF4 gene can serve as a heat-resistant gene. The corn ZmPIF4 gene can respond to high-temperature stress through up-down regulation expression, the corn ZmPIF4 gene can increase the GA content, JA content and IAA content of corn plants, gibberellin has the most prominent effect of accelerating cell elongation and promoting cell expansion, and therefore the high-temperature stress resistance of the corn plants can be improved.

This invention belongs to the field of lithium-ion battery technology and relates to a battery module with a prestressed composite constraint structure. A pre-tightening constraint layer, a rigid-flexible coupling constraint structure, and an outer constraint layer are sequentially distributed from the inside to the outside of the battery stack. The rigid-flexible coupling constraint structure is a continuous force-bearing body formed by a high-strength flexible load-bearing material and structural adhesive. Prestress is introduced into the pre-tightening constraint layer and the rigid-flexible coupling constraint structure during the winding process, forming a radial compressive constraint force field on the outer periphery of the battery. This structure achieves active control of cell expansion through a "prestress-expansion stress offsetting" mechanism and homogenizes stress distribution through a circumferential continuous force-bearing structure. Compared with existing technologies, this invention can significantly reduce module expansion and structural deformation, improve cycle stability and service life, while also possessing lightweight design and good process adaptability, making it suitable for various pouch battery modules.

Provided is a method of transducing and expanding a population of cells, the method comprising, in order: a cell selection step; a pre-transduction activation step; a cell transduction step; and a cell expansion phase. At least the cell transduction step and the expansion phase comprise incubation of the cells with IL-15. The methods of the invention are well suited to the transduction and expansion of populations of cells expressing chimeric antigen receptors (CARs), and in particular for the transduction and expansion of populations of invariant natural killer T (iNKT) cells expressing CARs. Also provided are populations of cells produced by the methods of the invention, and pharmaceutical compositions comprising populations of cells, as well medical uses of the pharmaceutical compositions and populations of cells. The cells and pharmaceutical composition are suitable for application in medical use and methods of treatment, including immunotherapy.

Provided herein are vaccine compositions for use in the stimulation of broad-spectrum memory B cell expansion in a patient that has been exposed or is at risk of exposure to an infectious agent of unknown etiology. The composition comprises IL-15 or an IL-15 analog and at least two toll-like receptor (TLR) agonists, wherein the agonists are targeted to different members of the TLR family. Further contemplated herein are compositions and methods of stimulating germinal B cell expansion in a patient, the composition comprising IL-15 or an IL-15 analog and a nucleic acid, wherein the nucleic acid encodes at least one immunogenic peptide, and wherein the composition is administered either subcutaneously or directly into a lymph node.

The invention provides a thermal barrier assembly for a traction battery pack. A thermal barrier assembly for managing thermal energy transfer within a traction battery pack is provided. An exemplary thermal barrier assembly may be axially disposed between battery cells of a battery array and may include a thermal suppression container having an outer pouch and a thermal suppressor releasably housed within the outer pouch, and at least one cell expansion pad located inside or outside the outer pouch. The thermal suppression container may be configured to release a thermal suppression agent when a temperature near the outer bag exceeds a predefined temperature threshold. The thermal inhibitor may capture or trap particles associated with battery exhaust byproducts, managing or even preventing transfer of thermal energy to nearby structures. The cell expansion pad may be configured to accommodate a compressive force and an expansion force of the battery cell.

Disclosed herein are methods of administering immunotherapy. In some embodiments, the methods include administering cells to a subject, and subsequent expansion of the cells in peripheral blood. In some embodiments, the methods use blood cells to promote the amplification. The method can be used to treat cancer.

Three dimensional (3D) bioreactors for the large-scale expansion of immune cells and methods of use thereof are provided.SOLUTION: 3D bioreactors comprise at least one packed bed chamber comprising at least one microporous scaffold, at least one microporous scaffold coated with one or more extracellular matrix proteins (ECMs), at least one vessel comprising a fluidic media, wherein the fluidic media is configured to flow through the packed bed chamber having the at least one microporous coated scaffold, and at least one population of immune cells suspended in the fluidic media. The at least one porous scaffold coated with ECM forms an anchoring niche with low shear forces that mimics the natural growth environment of immune cells. This allows for large scale expansion of the immune cell population flowing through the coated porous scaffold.SELECTED DRAWING: FIG. 2 (1)

The utility model belongs to the field of batteries, and particularly relates to a heat management structure and a battery cell module. The heat management structure comprises a liquid cooling plate and expansion ribs arranged in the liquid cooling plate. Cavities formed among the expansion ribs are used as flow channels of liquid coolants; the expansion rib comprises a connecting rib and a limiting rib; the limiting ribs are arranged on the upper wall and / or the lower wall of the liquid cooling plate, the height of the limiting ribs does not exceed the distance between the upper wall and the lower wall, and the limiting ribs limit the upper wall and the lower wall; the connecting ribs are connected with the upper wall and the lower wall of the liquid cooling plate; or the connecting ribs are connected to the limiting ribs. The heat management structure disclosed by the utility model is formed by combining different connecting ribs and limiting ribs, so that the expansion force of the battery cells can be absorbed, the problem that the runners are crushed after the heat management structure is expanded and extruded by the battery cells is solved, and meanwhile, the problem that liquid coolants cannot pass through the battery cells after the battery cells are expanded is also solved.

A substrate for culturing cells that comprises a modified prolamin having a net positive charge is provided. The modified prolamin may be a prolamin that has been modified through amidation or esterification, such as the addition of a methyl-ester group or an ethanolamine group. The modified prolamin is animal-free may be food-grade and / or may be free of cross-linkers. The modified prolamin may be in a coating that can be applied to a surface for culturing adherent cells, such as a microcarrier (e.g., beads) or well plates. Substrates coated with the modified prolamin are able to support cell expansion and can be used for food-grade applications such as cultured meat.

This invention belongs to the field of biomedical technology, specifically relating to a method and application for preparing CAR-T cells with enhanced persistence based on the piggyBac transposon system combined with electroporation. This invention, through specific piggyBac dual plasmid ratios and electroporation conditions, can improve transfection efficiency to over 80%, and the integration site is more likely to be located in a safe genomic region, supporting long-term stable CAR expression. Furthermore, cell expansion kinetics are significantly improved, with expansion exceeding 120-fold within 15 days. The results of the examples show that CAR-T cells prepared using the method described in this invention exhibit significantly enhanced tumor cell killing ability and cytokine secretion levels in in vitro models, and are rich in memory T cell subsets (Tcm / Tem) with greater persistence and self-renewal potential. This indicates that this invention not only changes the method of gene delivery but also shapes the intrinsic properties of cells, enabling them to acquire potentially superior in vivo persistence and long-term anti-tumor capabilities.

The invention belongs to the technical field of cell culture, and particularly relates to a method for preparing Jurkat cells on a large scale by utilizing a bioreactor. The preparation method comprises the following steps: (1) cell resuscitation: resuscitating cells from liquid nitrogen; (2) carrying out cell multiplication culture: carrying out step-by-step amplification multiplication culture in T bottles and cell culture bags with different specifications; (3) large-scale culture of cells: performing large-scale culture by using a bioreactor; and (4) harvesting the cells: collecting the cell suspension, and centrifugally harvesting the cells. The preparation method overcomes the problem that a large number of thymus cells or human lymphocytes need to be used as immunogens when the anti-human T lymphocyte immune globulin is prepared, can simply and quickly prepare a large number of Jurkat cells, is used for preparing the anti-human T lymphocyte immune globulin, improves the quality, reduces the biological safety risk, and has a wide application prospect. The method is beneficial to industrial production and has a relatively good economic effect.

The embodiment of the invention provides a cell factory reactor and a use method, and relates to the technical field of cell culture, the cell factory reactor comprises: a cell factory main body having a plurality of culture layers and a first channel respectively communicated with the plurality of culture layers, each culture layer having an upper surface and a lower surface, both the upper surface and the lower surface being subjected to TC treatment; the sensing assembly is used for acquiring culture environment parameters in the cell factory main body; the process gas supply assembly is connected with the first channel; the pipeline system comprises a liquid input assembly and a liquid output assembly, the liquid input assembly is used for conveying materials required by cell culture to the first channel, and the liquid output assembly is used for transferring liquid or cell suspension in the cell factory main body to the outside of the cell factory main body; the controller comprises a swinging mechanism, and the swinging mechanism is used for driving the cell factory main body to swing. According to the embodiment of the invention, the scale of cell amplification is increased, and the problem of insufficient amplification scale of a traditional cell factory is effectively solved.

This invention discloses the application of substances that increase LSH content or activity in improving heterochromatin stability and enhancing the efficacy of cell therapy, belonging to the field of biomedical technology. This invention, through overexpression of LSH in IMR90 fibroblasts and T lymphocytes, successfully achieved for the first time complete inhibition of DNA methylation loss in heterochromatin regions during cell expansion. The innovative research results of this invention strongly demonstrate that the insufficient DNA methylation maintenance efficiency caused by the dense chromatin environment in heterochromatin regions is the reason for the continuous loss of DNA methylation during sustained cell expansion. Overexpression of LSH can effectively improve the DNA methylation maintenance efficiency in heterochromatin regions to prevent DNA methylation loss, thereby improving several phenotypic defects caused by sustained cell expansion. This provides a new strategy for effectively maintaining cell function in adoptive cell therapy and has broad application value in enhancing the efficacy of various cell therapies.

The utility model provides a kind of power battery multi-module folding type welding cold plate structure, it is related to new energy battery technical field, including module cold plate assembly, first module cold plate and second module cold plate, the both sides of module cold plate assembly are equipped with edge, the front of edge is equipped with water outlet, the back of edge is equipped with water inlet, the end of water inlet and water outlet is equipped with sealing ring, the outside of module cold plate assembly includes cold plate outer wall, power battery multi-module folding type welding cold plate structure is designed by three kinds of assembly forms of module cold plate assembly, and the cooling demand of different battery module can be flexibly adapted. In the first form, first module cold plate and second module cold plate are integrated by welding and form sealing, internal S-shaped support septum is connected to form limiting step, provide high rigid support, suitable for high-strength battery module scene;The flexible of the second form of partition type design and reserved gap is enhanced, and the large deformation caused by cell expansion is adapted.

A separator structure for mitigating thermal energy transfer inside a traction battery pack is provided. A battery system for a traction battery pack is provided. An exemplary battery system may include a first cell stack, a second cell stack, and a separator disposed between the first cell stack and the second cell stack. The separators may be configured to mitigate transfer of thermal energy from the cell stack to the cell stack, structurally integrate the battery system into a peripheral structure, suppress displacement of the cells due to external forces, resist cell expansive forces, and the like.

The application relates to the technical field of stem cell culture, and provides a fusion degree evaluation method and equipment for stem cell expansion culture, which comprises the following steps: calculating a microenvironment feature by using a microenvironment parameter and a stem cell fusion degree parameter; obtaining a time perception label by performing time perception based on a two-dimensional image, and obtaining a fusion degree prediction label by performing fusion degree classification; fusing a two-dimensional image and a three-dimensional image based on the time perception label and the fusion degree prediction label to obtain an enhanced stem cell image; performing time perception on the enhanced stem cell image to obtain an enhanced time perception label, and performing fusion degree evaluation to obtain an enhanced fusion degree prediction label; and performing fusion degree evaluation by using a fusion degree evaluation model based on the microenvironment feature, the time perception label, the fusion degree prediction label, the enhanced time perception label and the enhanced fusion degree prediction label, so as to obtain a fusion degree evaluation value. The method can improve the accuracy of fusion degree evaluation.

The utility model discloses a cell culture carrier capable of recycling cells, and relates to the technical field of exosome production and cell recycling, the cell culture carrier comprises a cell growth layer used for cell growth, the outer wall of the cell growth layer is provided with a thin film, the outer wall of the thin film is provided with a thick film, and the thick film is provided with a plurality of cells. According to the cell culture carrier capable of recycling the cells, after cell amplification reaches a plateau phase, 10%-90% of intra-membrane cells can dissociate and fall off from the side wall of a membrane by increasing the rotating speed without a digestion process, and the viability of the free cells is greater than 80%; the purpose of cell recovery can be achieved through operations such as centrifugation, and the cell amplification and recovery process is greatly simplified; the intra-membrane cells can be continuously amplified and harvested by continuously facilitating falling and updating, meanwhile, the yield per unit volume of the exosome after the cell growth layer is improved is also improved to a certain extent compared with the yield of the exosome of a common structure (two layers of membranes and one layer of spacing net) in the market, and the total yield can be improved through multi-batch harvesting.