309 results about "Cell expansion" patented technology

The invention discloses an energy storage power station fire early warning method and system based on multi-parameter fusion, and the method comprises the following steps: collecting the temperature, characteristic gas concentration, cell expansion force, voltage fluctuation and environment temperature and humidity data of a lithium battery of an energy storage power station in real time through a distributed sensor, and carrying out the cleaning, denoising and standardization processing of the collected parameters, temperature and gas concentration monitoring values are corrected through an environment temperature and humidity compensation algorithm, abnormal data caused by environment interference are eliminated, the preprocessed data are input into a preset intelligent early warning module, and the model is based on a random forest algorithm. Through multi-parameter fusion and intelligent algorithm deep analysis, in combination with environment compensation, interference elimination, early warning accuracy improvement, graded early warning and linkage response, full-stage accurate disposal is achieved, timeliness is enhanced, sensor redundancy, multi-cabin cooperation and other mechanisms guarantee reliability, a closed loop from monitoring to disposal is formed, and the fire risk and loss are effectively reduced.

The invention relates to a Bayesian optimization-based ruthenium, tin and indium doped lithium-rich manganese-based electrocatalyst screening method, electronic equipment and a storage medium, and the method comprises the following steps: generating a uniform ruthenium, tin and indium doped lithium-rich manganese-based material data set based on Dirac distribution, and generating a doped structure through element replacement; calculating hydroxyl adsorption energy and extracting descriptors to construct a data set to train a machine learning model; performing cell expansion operation on the initial electrocatalyst structure, predicting hydroxyl adsorption energy of each adsorption site, and mapping adsorption energy deviation into current density characterization component activity in combination with Boltzmann distribution; selecting an initial component based on the obtained component activity information, and constructing an agent model by utilizing Bayesian optimization; and determining a to-be-sampled component through an expectation improvement strategy, sampling and updating the agent model, and outputting an optimal doping proportion. Compared with the prior art, the method has the advantages that the ruthenium, tin and indium doped lithium-rich manganese-based electrocatalyst with excellent catalytic performance can be efficiently screened out, and the experiment cost is reduced.

The invention relates to the technical field of biological medicine, in particular to a hepatitis A inactivated vaccine and a preparation method thereof. Comprising the following steps: cell amplification, virus culture, virus harvesting, cell disruption, ultrafiltration concentration, inactivation, purification and finished product obtaining. The virus culture comprises the following steps: separating from excrement of a hepatitis A patient and carrying out adaptive passage to obtain a virus strain; adding a carrier into a bioreactor, sterilizing for later use, inoculating a virus strain into a collected cell suspension, and transferring into the bioreactor for culturing; the cell disruption step comprises the following steps: carrying out ultrasonic oscillation on a collected virus harvesting liquid to obtain a cell disruption liquid; trichloromethane is added for extraction and centrifugation, and supernatant virus liquid is collected; the invention provides a preparation method of a hepatitis A inactivated vaccine, which is used for preparing the efficient, safe and stable hepatitis A inactivated vaccine by optimizing virus culture and inactivation processes. The method has the advantages of high virus yield and low production cost, and is suitable for large-scale production and application.

The invention discloses a cord blood-derived regulatory T cell amplification culture medium and a use method thereof, and relates to the field of animal cell culture and immune cell therapy, the culture medium is a serum-free culture medium, the culture medium is composed of a basic culture medium, a serum substitute combination, a combination of four cell factors, an immunosuppressor, an apparent stabilizer and an antioxidant, and all the components play a role synergistically; according to the use method, cell amplification is realized through a collaborative process of staged environmental regulation and targeted activation. According to the method, the problems of exogenous pollution and batch difference caused by dependence of a serum-containing system on cord blood-derived regulatory T cell amplification in the prior art are solved, the defect that a serum-free scheme is difficult to consider the amplification efficiency and the function stability at the same time is overcome, the cell amplification quality and the clinical application safety are guaranteed, and the method is suitable for large-scale clinical transformation requirements.

The invention belongs to the field of battery state monitoring, and discloses a battery thermal runaway early warning method based on a single-point film pressure sensor, and the method comprises the steps: arranging the single-point film pressure sensor between adjacent cells in a battery pack, and sensing a cell pressure signal in real time; the charging and discharging process of the battery pack is monitored through the battery cell pressure signals, the cycle pressure peak value sequence and the cycle pressure valley value sequence of the latest N charging and discharging cycle periods of each battery cell in the charging and discharging process are recorded in a self-adaptive mode, and the dynamic grading pressure early warning threshold value of each battery cell is calculated according to the cycle pressure peak value sequence and the cycle pressure valley value sequence; and carrying out battery cell abnormity identification and thermal runaway early warning according to the dynamic grading pressure early warning threshold. According to the method, the interference of factors such as cell manufacturing tolerance, sensor individual difference, installation deviation and cell expansion caused by charging and discharging circulation is effectively counteracted through the dynamic grading threshold value of each cell, so that the early warning result is more fit with the actual state of the battery, and a reliable basis is provided for early identification of thermal runaway.

The invention relates to the technical field of computers, in particular to a multi-scale automatic analysis method for an amorphous material atomic structure. Comprising the following steps: S1, taking a reactant as an initial unit, and obtaining a cluster unit of a reaction product based on first principle molecular dynamics simulation; s2, carrying out unit cell construction on the cluster unit, and optimizing unit cell parameters and atomic coordinates of the cluster unit through calculation according to a first principle to obtain unit cell units; s3, spatial stacking is carried out on the unit cell units, unit cell parameters and atomic coordinates of the unit cell units are optimized through calculation according to a first principle, and supercell units are obtained; s4, performing first principle molecular dynamics simulation on the supercell unit to obtain an accurate machine learning potential function; s5, carrying out cell expansion construction on the supercell unit, carrying out molecular dynamics simulation by using an accurate machine learning potential function, and obtaining an amorphous material atomic structure; and S6, packaging the steps S1 to S5 into an automatic workflow. The method is high in efficiency and small in error.

The invention relates to the technical field of biology, in particular to a Treg cell amplification culture medium and a preparation method of Treg cells. According to the method for in-vitro amplification of the regulatory T cells (Treg) from peripheral blood, the amplification efficiency and purity of the Treg cells are improved, the culture cost is reduced, the culture period is shortened, and the problem of trophoblast cell residues in subsequent clinical application is solved.

The embodiment of the invention discloses an aluminum honeycomb cell expansion CAE model parameter calibration method based on machine vision. The method comprises the steps that initial model parameters are selected and substituted into a target aluminum honeycomb cell expansion CAE model, a rigidity-displacement characteristic curve and a strain cloud picture are obtained through calculation, then a rigidity criterion in the rigidity-displacement characteristic curve is determined based on a stable deformation interval, the strain cloud picture is extracted based on machine vision image standard normalization processing to determine a deformation mode criterion, and the deformation mode criterion is determined. And finally, continuously iterating the model parameters of the target aluminum honeycomb cell expansion CAE model through the rigidity criterion and the deformation mode criterion until both the rigidity criterion and the deformation mode criterion meet preset requirements. According to the automatic calibration steps, manual calibration intervention is not needed, calibration of the aluminum honeycomb cell expansion CAE model can be completed by using a small number of iteration steps, the model calibration time is saved, an aluminum honeycomb block crushing deformation mode in the calibration process is quantified in an image machine vision mode, and the calibration process efficiency meets the actual test requirement.

A cell module for an electrical energy storage device of a motor vehicle includes at least one cell structure made up of energy storage cells, a metallic cell module frame, enclosing the at least one cell structure, for clamping the energy storage cells, and a measuring device for detecting a deformation of the cell module frame related to cell expansion. The measuring device includes a passive resistive extension sensor having a deformation-dependent electrical resistance and a measuring unit connected to the extension sensor to output an electrical measurement signal characterizing the deformation-dependent electrical resistance of the extension sensor. The extension sensor is formed by the metallic cell module frame, with which the measuring unit is electrically contacted.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Thermal barrier systems are provided for inhibiting the transfer of thermal energy inside a traction battery pack. An exemplary thermal barrier system may include one or more thermal barrier assemblies arranged between adjacent battery cell groups of a cell stack. Each thermal barrier assembly may include a mica core, a first ceramic foam layer, a second ceramic foam layer, a first glass silicone layer, and a second glass silicone layer. In some implementations, the thermal barrier system may additionally include one or more cell expansion pad assemblies and / or one or more thermal barriers.

The invention relates to a diaphragm, a preparation method thereof and a secondary battery, and belongs to the technical field of secondary batteries. The diaphragm provided by the invention comprises a base material and a polymer coating arranged on at least one side of the base material, wherein the polymer coating comprises at least two coating belts which are distributed at intervals; a coating tape in the polymer coating contains a main polymer and an auxiliary polymer; the main polymer forms a porous structure layer; the auxiliary polymer is embedded into the porous structure layer, and bulges are formed on the surface of the porous structure layer. The diaphragm is prepared by adopting a specific method, and a secondary battery prepared by hot pressing has good liquid retention capacity, low cell expansion rate, high capacity retention rate, capability of remarkably improving the problem of dendritic crystal precipitation in a corner area, balanced cell performance and very high application value.

The present invention relates to a novel identified stem cell vMSC having angiogenesis characteristics, the vMSCs identified after being cultured under the culture conditions of the present invention are different from existing MSCs, are novel MSCs expressing CD141 cell surface antigens, have excellent cell expansibility, have an effect of directly forming blood vessels, have a paracrine effect of other MSCs, and are different from existing stem cells. The stem cell therapeutic agent for revascularization has a higher survival rate and a higher residual rate in tissues than conventional stem cell therapeutic agents, and can be used as a stem cell therapeutic agent for revascularization in which blood vessels are directly formed.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

Disclosed herein are methods of administering an immunotherapy. In some embodiments, the method comprises administration of a cell into a subject and the subsequent expansion of that cell in the peripheral blood. In some embodiments, the method employ blood cells to fuel the expansion. This method may be used in the treatment of cancer.

The invention provides a cell capacity expansion method, electronic equipment, a storage medium and a program product, relates to the technical field of communication, and is used for improving the network performance of a cell. The method comprises the following steps: acquiring a physical resource block (PRB) utilization rate interval distribution index of a target cell in a statistical period; the PRB utilization rate interval distribution index is used for indicating the distribution number and / or distribution proportion of the PRB resource utilization rate of each sampling period in a plurality of predefined intervals in a plurality of sampling periods of the target cell in the statistical period; and expanding the capacity of the target cell under the condition that the target cell is determined to be in a high-load state based on the PRB utilization rate interval distribution index.

The invention provides a construction method and application of a gene modified K562 cell line, and belongs to the technical field of biological medicines. According to the invention, two viruses CD86-4-1BBL and mbIL-21 are respectively subjected to gene transduction packaging by adopting a third-generation lentivirus four-plasmid packaging system, a K562 cell line is infected by the lentiviruses CD86-4-1BBL and mbIL-21, and a monoclonal cell line capable of stably expressing CD86, 4-1BBL and mbIL-21 proteins is obtained through screening. According to the method, cell lines with the cloning numbers of V5-E9, V10-A5, V5-D6 and V6-D7 are obtained through screening, the four cloning cell lines can serve as feeder cells for stimulating NK cell amplification after being amplified and subjected to irradiation treatment, NK cell amplification can be effectively promoted, the amplification multiple of the NK cells reaches up to 6000-8000, and large-scale preparation and production of the NK cells for treatment are met.

The present invention relates to a method for rapidly preparing chimeric antigen receptor T cells (CAR-T), comprising the steps of: 1) contacting T cells or a cell population comprising the T cells with a stimulating agent to activate the T cells to obtain activated T cells or a cell population comprising the activated T cells; 2) contacting the activated T cell or a cell population comprising activated T cells with a nucleic acid molecule encoding a chimeric antigen receptor (CAR), thereby obtaining a CAR-T cell expressing the CAR; and (3) harvesting the CAR-T cells, culturing for 18-24 hours, harvesting after the culture is finished, and freezing or not freezing. According to the method, the total preparation time of the CAR-T cells is greatly shortened, and meanwhile, the amplification and tumor killing capabilities of the CAR-T cells are remarkably improved.

The invention discloses a gamma delta T cell efficient amplification method and application, and belongs to the technical field of cell biology. The amplification method comprises the following steps: separating PBMC (peripheral blood mononuclear cells) by adopting a density gradient centrifugation method, carrying out initial culture through a polylysine coated container, carrying out three-stage dynamic stimulation, combining with an X-VIVO 15 culture medium of 5-8% autoserum, and finally carrying out anti-gamma delta TCR magnetic bead separation to obtain high-purity cells. Through collaborative optimization of stepped factor combination, staged container coating and a low-serum system, the amplification multiple of the gamma delta T cells reaches 150-180 times, the purity is larger than or equal to 90% after purification, the cytotoxicity and the survival ability are remarkably improved, the gamma delta T cells can be efficiently used for immunotherapy of tumors and infectious diseases, and stable technical support is provided for clinical transformation of the gamma delta T cells.

The invention provides a lithium ion battery health state estimation method and system based on mechanical information and a medium, and the method comprises the steps: carrying out the full-life test of a battery cell of a lithium ion battery based on experimental parameters, and obtaining the experimental data of the battery cell; preprocessing the battery cell experiment data, constructing an expansion curve based on the optimized experiment data, extracting battery cell expansion characteristics, and analyzing battery cell response information; constructing a lithium ion battery health state prediction model, and analyzing lithium ion battery capacity attenuation state information according to the battery health state prediction model; analyzing battery health state information based on the lithium ion battery capacity attenuation state information, and analyzing battery performance according to the battery health state information to obtain performance evaluation information; the battery cell response information is calculated by analyzing the battery cell expansion curve, the lithium ion battery capacity attenuation state is analyzed according to the constructed lithium ion battery health state prediction model, the battery health state change is accurately analyzed, the battery performance is accurately analyzed, and the analysis precision is improved.

There is provided a hydrogel, such as a functionalized hydrogel, for use in the expansion and / or differentiation of cells, that are encapsulated within the three-dimensional structure of the hydrogel. In some aspects of the invention, the hydrogel better mimics the thymus microenvironment than prior hydrogels for T cell expansion and differentiation and can be used in the expansion and differentiation of HSPCs, HSCs or PCs or combinations thereof in the production of T cells, T-cell committed precursors or progenitor cells and in methods for producing mature T cells. Methods of making the hydrogels, methods of culturing the cells in the hydrogel and uses of the hydrogels and cells produced are also described.

The invention relates to a vanadium oxide coated and zirconium-doped modified precursor and a preparation method and application thereof.According to the preparation method, zirconium doping and an ammonium vanadate solution are specifically used, cation mixing is inhibited through the stabilization effect of zirconium, lattice distortion is reduced, the stable unit cell volume can be maintained through zirconium doping, and the stability of the unit cell volume is improved; and the problem of unit cell expansion caused by conventional doping elements is avoided, so that the high-rate performance of the material is reserved. The semiconductor-metal phase change characteristic of vanadium oxide is utilized, the critical temperature can be adjusted to be close to the room temperature, and the high-temperature stability and electronic conductivity of the material are improved; through the combination of the two, the high-rate discharge performance of the ternary positive electrode material and the structural stability of the material can be greatly improved.

The invention provides a dynamic regulation and control method for optimizing CAR-T cell amplification conditions, which comprises the following steps: acquiring high-frequency cell activity monitoring data from a cell culture system, and extracting metabolic marker fluctuation and proliferation rate mutation from the high-frequency cell activity monitoring data to obtain real-time cell state parameters; comparing the real-time cell state parameters with a T cell subset proportion and a cell factor release mode in historical immune data of the patient to obtain a cell state deviation value, and determining an abnormal fluctuation condition of proliferation rate mutation according to the cell state deviation value; if the abnormal fluctuation of the proliferation rate mutation exceeds a preset fluctuation threshold value, capturing proliferation signal abnormity by analyzing metabolic marker fluctuation and metabolic waste accumulation according to the cell state deviation value; according to the targeted culture parameter set, adjusting the oxygen supply quantity and the culture solution microenvironment of an oxygen concentration control system to obtain a culture environment for inhibiting cytokine storm and proliferation signal abnormity.

1. Name of the design product: bioreactor (2L). 2. Use of the design product: for plant cell expansion, cell therapy, antibody drug cell expansion. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view 1.

The invention discloses a method for culturing NK (Natural Killer) cells. The method comprises the following steps: inoculating a mononuclear cell into a T25 cell culture flask, wherein the T25 cell culture flask is coated by a coating factor in advance; carrying out culture treatment on the inoculated mononuclear cells in a culture system containing an NK cell culture medium containing an activation amplification factor and plasma so as to obtain NK cells; wherein the coating factor, the activation amplification factor and the NK cell culture medium are derived from an NK cell amplification kit. According to the method provided by the embodiment of the invention, the dosage of the amplification reagent can be reduced, the amplification multiple of the NK cells is improved, and the production cost is reduced.

The lithium battery for the unmanned aerial vehicle comprises a shell and at least two battery cells arranged in the shell side by side, a partition plate is arranged between every two adjacent battery cells, an avoiding hole for an expansion reserved space of the battery cells is formed in the middle of each partition plate, a positive pole piece and a negative pole piece are arranged on each battery cell, and the positive pole pieces and the negative pole pieces are arranged on the shell. The shell is provided with a positive pole hole for the positive pole piece to penetrate out, the shell is provided with a negative pole hole for the negative pole piece to penetrate out, a first welding disc for welding the positive pole piece and the negative pole piece is arranged between the negative pole hole for the negative pole piece of the previous battery cell to penetrate out and the positive pole hole for the positive pole piece of the next battery cell to penetrate out, and the first welding disc is connected to the shell. And the avoiding hole formed in the middle of the partition plate provides a reserved space required by expansion for the battery cell, so that the problem of overall deformation or damage of the battery caused by expansion of the battery cell is avoided, and the safety and the service life of the battery are favorably improved. The positive and negative plates between the adjacent battery cells are connected by introducing the first welding disc, so that the welding process is simplified.

Provided herein are methods for producing an isolated population of prepared NK cells. In particular embodiments, prepared NK cells are expanded for a certain duration of time. In particular embodiments, prepared NK cells are expanded in one or more certain media(s). Further provided herein are compositions for preparing NK cells, compositions comprising prepared NK cells, and methods for the treatment of disease by administering the prepared NK cells.

The present invention relates to the field of bioengineering, and in particular to the application of genetically modified mesenchymal stem cells in natural killer cell culture. The IL-15 gene-modified umbilical cord mesenchymal stem cells prepared by the present invention have a strong factor secretion ability, can be amplified with the proliferation of NK cells, and can continuously and stably secrete IL-15, effectively improving the in vitro amplification efficiency of NK cells, enhancing the tumor killing function of NK cells, and also avoiding the additional addition of cytokines during the NK cell amplification culture process, avoiding the pollution caused by frequent rehydration operations, and also greatly reducing the production cost.

The invention relates to a polylactic acid microcarrier and a method for cell amplification by using the polylactic acid microcarrier. The preparation method disclosed by the invention is simple and convenient to operate, the prepared polylactic acid microcarrier is uniform in particle size and pore size distribution, the microcarrier is subjected to surface retreatment, more active sites are provided for cell adhesion, a three-dimensional matrix environment closer to a body is provided, cell amplification is facilitated, the dryness and differentiation potential of stem cells are maintained, and the preparation method is suitable for large-scale popularization and application. And the microcarrier can be repeatedly used, so that the use cost is reduced.

The invention provides a cell strain for producing engineered viroid particles, a method thereof and the engineered viroid particles, the cell strain is named as SZCV-Con28 and preserved in Guangdong Microbial Culture Collection Center on October 15, 2025, the preservation number is GDMCC No: 67101, and the preservation number is GDMCC No: 67101. A plurality of corresponding cell factories for producing the engineered viroid particles are established by cell lines obtained by introducing different target gRNA elements into the cell strain, so that the corresponding engineered viroid particles can be continuously produced in batches. According to the invention, large-scale preparation can be realized through cell expanding culture, the capacity limitation is overcome, the production process flow is simplified, and the consumption of production raw materials is reduced.