182 results about "Cell expansion" patented technology

The invention discloses an energy storage power station fire early warning method and system based on multi-parameter fusion, and the method comprises the following steps: collecting the temperature, characteristic gas concentration, cell expansion force, voltage fluctuation and environment temperature and humidity data of a lithium battery of an energy storage power station in real time through a distributed sensor, and carrying out the cleaning, denoising and standardization processing of the collected parameters, temperature and gas concentration monitoring values are corrected through an environment temperature and humidity compensation algorithm, abnormal data caused by environment interference are eliminated, the preprocessed data are input into a preset intelligent early warning module, and the model is based on a random forest algorithm. Through multi-parameter fusion and intelligent algorithm deep analysis, in combination with environment compensation, interference elimination, early warning accuracy improvement, graded early warning and linkage response, full-stage accurate disposal is achieved, timeliness is enhanced, sensor redundancy, multi-cabin cooperation and other mechanisms guarantee reliability, a closed loop from monitoring to disposal is formed, and the fire risk and loss are effectively reduced.

The invention discloses a cord blood-derived regulatory T cell amplification culture medium and a use method thereof, and relates to the field of animal cell culture and immune cell therapy, the culture medium is a serum-free culture medium, the culture medium is composed of a basic culture medium, a serum substitute combination, a combination of four cell factors, an immunosuppressor, an apparent stabilizer and an antioxidant, and all the components play a role synergistically; according to the use method, cell amplification is realized through a collaborative process of staged environmental regulation and targeted activation. According to the method, the problems of exogenous pollution and batch difference caused by dependence of a serum-containing system on cord blood-derived regulatory T cell amplification in the prior art are solved, the defect that a serum-free scheme is difficult to consider the amplification efficiency and the function stability at the same time is overcome, the cell amplification quality and the clinical application safety are guaranteed, and the method is suitable for large-scale clinical transformation requirements.

The invention belongs to the field of battery state monitoring, and discloses a battery thermal runaway early warning method based on a single-point film pressure sensor, and the method comprises the steps: arranging the single-point film pressure sensor between adjacent cells in a battery pack, and sensing a cell pressure signal in real time; the charging and discharging process of the battery pack is monitored through the battery cell pressure signals, the cycle pressure peak value sequence and the cycle pressure valley value sequence of the latest N charging and discharging cycle periods of each battery cell in the charging and discharging process are recorded in a self-adaptive mode, and the dynamic grading pressure early warning threshold value of each battery cell is calculated according to the cycle pressure peak value sequence and the cycle pressure valley value sequence; and carrying out battery cell abnormity identification and thermal runaway early warning according to the dynamic grading pressure early warning threshold. According to the method, the interference of factors such as cell manufacturing tolerance, sensor individual difference, installation deviation and cell expansion caused by charging and discharging circulation is effectively counteracted through the dynamic grading threshold value of each cell, so that the early warning result is more fit with the actual state of the battery, and a reliable basis is provided for early identification of thermal runaway.

The invention relates to the technical field of biology, in particular to a Treg cell amplification culture medium and a preparation method of Treg cells. According to the method for in-vitro amplification of the regulatory T cells (Treg) from peripheral blood, the amplification efficiency and purity of the Treg cells are improved, the culture cost is reduced, the culture period is shortened, and the problem of trophoblast cell residues in subsequent clinical application is solved.

A cell module for an electrical energy storage device of a motor vehicle includes at least one cell structure made up of energy storage cells, a metallic cell module frame, enclosing the at least one cell structure, for clamping the energy storage cells, and a measuring device for detecting a deformation of the cell module frame related to cell expansion. The measuring device includes a passive resistive extension sensor having a deformation-dependent electrical resistance and a measuring unit connected to the extension sensor to output an electrical measurement signal characterizing the deformation-dependent electrical resistance of the extension sensor. The extension sensor is formed by the metallic cell module frame, with which the measuring unit is electrically contacted.

Thermal barrier systems are provided for inhibiting the transfer of thermal energy inside a traction battery pack. An exemplary thermal barrier system may include one or more thermal barrier assemblies arranged between adjacent battery cell groups of a cell stack. Each thermal barrier assembly may include a mica core, a first ceramic foam layer, a second ceramic foam layer, a first glass silicone layer, and a second glass silicone layer. In some implementations, the thermal barrier system may additionally include one or more cell expansion pad assemblies and / or one or more thermal barriers.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

The invention discloses a gamma delta T cell efficient amplification method and application, and belongs to the technical field of cell biology. The amplification method comprises the following steps: separating PBMC (peripheral blood mononuclear cells) by adopting a density gradient centrifugation method, carrying out initial culture through a polylysine coated container, carrying out three-stage dynamic stimulation, combining with an X-VIVO 15 culture medium of 5-8% autoserum, and finally carrying out anti-gamma delta TCR magnetic bead separation to obtain high-purity cells. Through collaborative optimization of stepped factor combination, staged container coating and a low-serum system, the amplification multiple of the gamma delta T cells reaches 150-180 times, the purity is larger than or equal to 90% after purification, the cytotoxicity and the survival ability are remarkably improved, the gamma delta T cells can be efficiently used for immunotherapy of tumors and infectious diseases, and stable technical support is provided for clinical transformation of the gamma delta T cells.

The invention provides a lithium ion battery health state estimation method and system based on mechanical information and a medium, and the method comprises the steps: carrying out the full-life test of a battery cell of a lithium ion battery based on experimental parameters, and obtaining the experimental data of the battery cell; preprocessing the battery cell experiment data, constructing an expansion curve based on the optimized experiment data, extracting battery cell expansion characteristics, and analyzing battery cell response information; constructing a lithium ion battery health state prediction model, and analyzing lithium ion battery capacity attenuation state information according to the battery health state prediction model; analyzing battery health state information based on the lithium ion battery capacity attenuation state information, and analyzing battery performance according to the battery health state information to obtain performance evaluation information; the battery cell response information is calculated by analyzing the battery cell expansion curve, the lithium ion battery capacity attenuation state is analyzed according to the constructed lithium ion battery health state prediction model, the battery health state change is accurately analyzed, the battery performance is accurately analyzed, and the analysis precision is improved.

The invention provides a dynamic regulation and control method for optimizing CAR-T cell amplification conditions, which comprises the following steps: acquiring high-frequency cell activity monitoring data from a cell culture system, and extracting metabolic marker fluctuation and proliferation rate mutation from the high-frequency cell activity monitoring data to obtain real-time cell state parameters; comparing the real-time cell state parameters with a T cell subset proportion and a cell factor release mode in historical immune data of the patient to obtain a cell state deviation value, and determining an abnormal fluctuation condition of proliferation rate mutation according to the cell state deviation value; if the abnormal fluctuation of the proliferation rate mutation exceeds a preset fluctuation threshold value, capturing proliferation signal abnormity by analyzing metabolic marker fluctuation and metabolic waste accumulation according to the cell state deviation value; according to the targeted culture parameter set, adjusting the oxygen supply quantity and the culture solution microenvironment of an oxygen concentration control system to obtain a culture environment for inhibiting cytokine storm and proliferation signal abnormity.

1. Name of the design product: bioreactor (2L). 2. Use of the design product: for plant cell expansion, cell therapy, antibody drug cell expansion. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view 1.

The invention discloses a method for culturing NK (Natural Killer) cells. The method comprises the following steps: inoculating a mononuclear cell into a T25 cell culture flask, wherein the T25 cell culture flask is coated by a coating factor in advance; carrying out culture treatment on the inoculated mononuclear cells in a culture system containing an NK cell culture medium containing an activation amplification factor and plasma so as to obtain NK cells; wherein the coating factor, the activation amplification factor and the NK cell culture medium are derived from an NK cell amplification kit. According to the method provided by the embodiment of the invention, the dosage of the amplification reagent can be reduced, the amplification multiple of the NK cells is improved, and the production cost is reduced.

The lithium battery for the unmanned aerial vehicle comprises a shell and at least two battery cells arranged in the shell side by side, a partition plate is arranged between every two adjacent battery cells, an avoiding hole for an expansion reserved space of the battery cells is formed in the middle of each partition plate, a positive pole piece and a negative pole piece are arranged on each battery cell, and the positive pole pieces and the negative pole pieces are arranged on the shell. The shell is provided with a positive pole hole for the positive pole piece to penetrate out, the shell is provided with a negative pole hole for the negative pole piece to penetrate out, a first welding disc for welding the positive pole piece and the negative pole piece is arranged between the negative pole hole for the negative pole piece of the previous battery cell to penetrate out and the positive pole hole for the positive pole piece of the next battery cell to penetrate out, and the first welding disc is connected to the shell. And the avoiding hole formed in the middle of the partition plate provides a reserved space required by expansion for the battery cell, so that the problem of overall deformation or damage of the battery caused by expansion of the battery cell is avoided, and the safety and the service life of the battery are favorably improved. The positive and negative plates between the adjacent battery cells are connected by introducing the first welding disc, so that the welding process is simplified.

Provided herein are methods for producing an isolated population of prepared NK cells. In particular embodiments, prepared NK cells are expanded for a certain duration of time. In particular embodiments, prepared NK cells are expanded in one or more certain media(s). Further provided herein are compositions for preparing NK cells, compositions comprising prepared NK cells, and methods for the treatment of disease by administering the prepared NK cells.

The invention relates to a polylactic acid microcarrier and a method for cell amplification by using the polylactic acid microcarrier. The preparation method disclosed by the invention is simple and convenient to operate, the prepared polylactic acid microcarrier is uniform in particle size and pore size distribution, the microcarrier is subjected to surface retreatment, more active sites are provided for cell adhesion, a three-dimensional matrix environment closer to a body is provided, cell amplification is facilitated, the dryness and differentiation potential of stem cells are maintained, and the preparation method is suitable for large-scale popularization and application. And the microcarrier can be repeatedly used, so that the use cost is reduced.

The invention provides a cell strain for producing engineered viroid particles, a method thereof and the engineered viroid particles, the cell strain is named as SZCV-Con28 and preserved in Guangdong Microbial Culture Collection Center on October 15, 2025, the preservation number is GDMCC No: 67101, and the preservation number is GDMCC No: 67101. A plurality of corresponding cell factories for producing the engineered viroid particles are established by cell lines obtained by introducing different target gRNA elements into the cell strain, so that the corresponding engineered viroid particles can be continuously produced in batches. According to the invention, large-scale preparation can be realized through cell expanding culture, the capacity limitation is overcome, the production process flow is simplified, and the consumption of production raw materials is reduced.

An engineered trophoblast that expresses a cell surface molecule that specifically recognizes NKp46, the cell surface molecule comprising at least an antibody targeting NKp46 and a transmembrane domain. The invention discloses a cell culture medium and a method for amplifying NK cells. Compared with unengineered trophoblasts, the engineered trophoblasts are used for activating NK cells, and the amplification capacity of the NK cells and the killing activity of tumor cells can be enhanced.

This invention discloses a flexible battery module thermoforming bracket and protection method with adaptive cell expansion, belonging to the field of thermoforming bracket technology. It includes a thermoforming bracket body, cell positioning cavities, an adaptive mechanism, an adjustment mechanism, a detection and movement mechanism, and a buffer mechanism. Sixteen cell positioning cavities are located at the top center of the thermoforming bracket body. The adaptive mechanism uses springs, electrical springs, resistance strain gauges, telescopic rods, and spherical blocks to actively and adaptively buffer cell expansion, preventing permanent failure of the springs and monitoring the expansion force to avoid cracking and deformation of the thermoforming bracket body. The adjustment mechanism dynamically adjusts the internal dimensions of the cell positioning cavities according to the cell expansion state by inflating and deflating the airbags, actively adapting to cell expansion and contraction, preventing cracking and deformation of the thermoforming bracket body, maintaining pre-tight positioning of the cells, preventing short circuits, and improving the structural protection of the thermoforming bracket body.

Chimeric antigen receptors (CARs) with binding domains derived from a novel suite of human CD33-binding antibodies are described. The CARs include optimized short and intermediate spacer regions. The current disclosure also provides methods of cell expansion / activation processes utilizing IL-2, IL-7, IL-15, and / or IL-21 that improve cellular proliferation and cell lysis of the CARs as described.

The invention relates to the technical field of gene engineering, in particular to a ZmPIF4 gene with heat resistance and application of the ZmPIF4 gene, the nucleotide sequence of the ZmPIF4 gene is shown as SEQ ID NO.1, the ZmPIF4 gene is mainly located in a cell nucleus and possibly plays an important role in the cell nucleus signal transduction process, and under high temperature stress, the ZmPIF4 gene can serve as a heat-resistant gene. The corn ZmPIF4 gene can respond to high-temperature stress through up-down regulation expression, the corn ZmPIF4 gene can increase the GA content, JA content and IAA content of corn plants, gibberellin has the most prominent effect of accelerating cell elongation and promoting cell expansion, and therefore the high-temperature stress resistance of the corn plants can be improved.

The application relates to the fields of biological medicine and computing simulation, and specifically discloses an immune cell expansion process simulation and process parameter optimization system, which collects multiple groups of candidate process parameters, introduces process disturbance, generates a dynamic trajectory set of living cell density, viability and metabolic by-products, and converts the dynamic trajectory set into a first probability distribution; calculates an entropy regularization distribution distance between the first probability distribution and a second probability distribution corresponding to ideal expansion kinetics as a matching degree measurement; constructs a difference response surface with the candidate process parameters and the distance value as samples, takes the current optimal distance as a benchmark, and calculates potential expected values of each candidate parameter by using the response surface; iteratively selects the parameter with the maximum potential expected value until the distance reduction amplitude is lower than a threshold value, and outputs the optimal process parameter; the application can avoid local optimization, improve the global optimization, and reduce the simulation rounds through dynamic distribution matching and difference response surface iterative optimization.

The utility model relates to the field of cell culture equipment, in particular to a cell culture instrument for amplifying immune cells, which comprises a culture box body, and the heating pipes are mounted on two sides in the incubator body and are used for internal heating. According to the cell culture instrument for immune cell amplification disclosed by the utility model, through the arrangement of the culture box body, the heating pipe, the temperature sensor, the humidity sensor, the control box, the processor, the humidifier and the spraying assembly, the temperature and the humidity in the culture box body are detected through the temperature sensor and the humidity sensor; detected data are transmitted to the processor, then the data are transmitted to the heating pipe through the processor, constant temperature control is conducted on the interior, and when the humidity in the incubator body is higher than the highest threshold value, the processor controls the humidifier to convey moisture to be sprayed out of the spraying assembly, so that the humidity and temperature in the incubator body are controlled; therefore, the internal environment reaches the most suitable culture environment.

This invention belongs to the field of lithium-ion battery technology and relates to a battery module with a prestressed composite constraint structure. A pre-tightening constraint layer, a rigid-flexible coupling constraint structure, and an outer constraint layer are sequentially distributed from the inside to the outside of the battery stack. The rigid-flexible coupling constraint structure is a continuous force-bearing body formed by a high-strength flexible load-bearing material and structural adhesive. Prestress is introduced into the pre-tightening constraint layer and the rigid-flexible coupling constraint structure during the winding process, forming a radial compressive constraint force field on the outer periphery of the battery. This structure achieves active control of cell expansion through a "prestress-expansion stress offsetting" mechanism and homogenizes stress distribution through a circumferential continuous force-bearing structure. Compared with existing technologies, this invention can significantly reduce module expansion and structural deformation, improve cycle stability and service life, while also possessing lightweight design and good process adaptability, making it suitable for various pouch battery modules.

Provided is a method of transducing and expanding a population of cells, the method comprising, in order: a cell selection step; a pre-transduction activation step; a cell transduction step; and a cell expansion phase. At least the cell transduction step and the expansion phase comprise incubation of the cells with IL-15. The methods of the invention are well suited to the transduction and expansion of populations of cells expressing chimeric antigen receptors (CARs), and in particular for the transduction and expansion of populations of invariant natural killer T (iNKT) cells expressing CARs. Also provided are populations of cells produced by the methods of the invention, and pharmaceutical compositions comprising populations of cells, as well medical uses of the pharmaceutical compositions and populations of cells. The cells and pharmaceutical composition are suitable for application in medical use and methods of treatment, including immunotherapy.

The present invention belongs to the technical field related to semiconductor materials, and discloses a method and system for calculating energy bands of a GeSn alloy. The method comprises: obtaining a parameter file of structurally optimized Ge; performing cell expansion and Sn doping on the structurally optimized Ge, establishing a POSCAR input file of a target GeSn alloy, establishing a POTCAR input file of the GeSn alloy according to cutoff inner and outer diameters of Ge and cutoff inner and outer diameters of Sn, wherein the cutoff inner diameter of Ge has a value range of 1.80 to 1.90 and the outer diameter has a value range of 3.20 to 3.25, and the cutoff inner diameter of Sn has a value range of 1.40 to 1.50 and the outer diameter has a value range of 4.10 to 4.20, and running a vasp program to perform self-consistent calculation on the target GeSn alloy to obtain a band gap value and a band gap type of the target GeSn alloy. The present invention can accurately simulate the electronic structure of GeSn alloys at different concentrations and accurately calculate their energy bands.

The present disclosure relates, at least in part, to compositions comprising microgels and / or granular hydrogels with tailored surface biochemical properties that can serve, e.g., as artificial antigen-presenting cells (aAPCs) to mediate, e.g., T cell activation and expansion. Such microgels and / or granular hydrogels can be used to manufacture T cells for adoptive therapy in cancer treatment and tissue regeneration, and can be configured to serve, e.g., as a readily tunable and modular system to enable both rapid T cell expansion and control over T cell phenotype. Some aspects of the present disclosure provide methods and compositions for fabricating microgels and / or granular hydrogels, and modulating the surface properties of microgels and / or granular hydrogels by functionalizing, e.g., via layer- by-layer coating.

The present invention discloses a three-dimensional (3D) bioreactor for large-scale expansion of immune cells and a method of using the same. The 3D bioreactor includes at least one packed bed chamber including at least one porous scaffold, at least one porous scaffold coated with one or more extracellular matrix proteins (ECMs), at least one container containing a fluid medium, the container being configured such that the fluid medium flows through the packed bed chamber having at least one porous coated scaffold, and at least one population of immune cells suspended in the fluid medium. At least one porous scaffold coated with ECM forms a fixed niche having low shear forces that mimics the natural growth environment of immune cells. This enables large-scale expansion of the population of immune cells flowing through the coated porous scaffold.

Provided herein are vaccine compositions for use in the stimulation of broad-spectrum memory B cell expansion in a patient that has been exposed or is at risk of exposure to an infectious agent of unknown etiology. The composition comprises IL-15 or an IL-15 analog and at least two toll-like receptor (TLR) agonists, wherein the agonists are targeted to different members of the TLR family. Further contemplated herein are compositions and methods of stimulating germinal B cell expansion in a patient, the composition comprising IL-15 or an IL-15 analog and a nucleic acid, wherein the nucleic acid encodes at least one immunogenic peptide, and wherein the composition is administered either subcutaneously or directly into a lymph node.

The invention provides a thermal barrier assembly for a traction battery pack. A thermal barrier assembly for managing thermal energy transfer within a traction battery pack is provided. An exemplary thermal barrier assembly may be axially disposed between battery cells of a battery array and may include a thermal suppression container having an outer pouch and a thermal suppressor releasably housed within the outer pouch, and at least one cell expansion pad located inside or outside the outer pouch. The thermal suppression container may be configured to release a thermal suppression agent when a temperature near the outer bag exceeds a predefined temperature threshold. The thermal inhibitor may capture or trap particles associated with battery exhaust byproducts, managing or even preventing transfer of thermal energy to nearby structures. The cell expansion pad may be configured to accommodate a compressive force and an expansion force of the battery cell.

Disclosed herein are methods of administering immunotherapy. In some embodiments, the methods include administering cells to a subject, and subsequent expansion of the cells in peripheral blood. In some embodiments, the methods use blood cells to promote the amplification. The method can be used to treat cancer.